                         SEQUENCE LISTING

<110>  Aarhus Universitet
 
<120>  Lentivirus-derived nanoparticles comprising crisp/cas9 
       ribonucleoprotein complexes

<130>  74390PC01

<160>  113   

<170>  PatentIn version 3.5

<210>  1
<211>  76
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA1.0 non-targeting region

<400>  1
guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc cguuaucaac uugaaaaagu       60

ggcaccgagu cggugc                                                       76


<210>  2
<211>  86
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA2.0 non-targeting region

<400>  2
guuuaagagc uaugcuggaa acagcauagc aaguuuaaau aaggcuaguc cguuaucaac       60

uugaaaaagu ggcaccgagu cggugc                                            86


<210>  3
<211>  96
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA2.1 non-targeting region

<400>  3
guuuaagagc uaugcuggaa acagcauagc aaguuuaaau aaggcuaguc cguuaucaac       60

uuugcuggaa acagcaaagu ggcaccgagu cggugc                                 96


<210>  4
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  wt SpCas9 crRNA


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  4
nnnnnnnnnn nnnnnnnnnn guuuuagagc uaugcuguuu ug                          42


<210>  5
<211>  86
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  wt tracrRNA

<400>  5
ggaaccauuc aaaacagcau agcaaguuaa aauaaggcua guccguuauc aacuugaaaa       60

aguggcaccg agucggugcu uuuuuu                                            86


<210>  6
<211>  102
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA1


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  6
nnnnnnnnnn nnnnnnnnnn guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu uu                         102


<210>  7
<211>  112
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA2.0


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  7
nnnnnnnnnn nnnnnnnnnn guuuaagagc uaugcuggaa acagcauagc aaguuuaaau       60

aaggcuaguc cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu uu              112


<210>  8
<211>  122
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA2.1


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  8
nnnnnnnnnn nnnnnnnnnn guuuaagagc uaugcuggaa acagcauagc aaguuuaaau       60

aaggcuaguc cguuaucaac uuugcuggaa acagcaaagu ggcaccgagu cggugcuuuu      120

uu                                                                     122


<210>  9
<211>  96
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA1 Aff1

<400>  9
ccuucagcuc agugacagug guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugc                                 96


<210>  10
<211>  106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA2.0 Aff1

<400>  10
ccuucagcuc agugacagug guuuaagagc uaugcuggaa acagcauagc aaguuuaaau       60

aaggcuaguc cguuaucaac uugaaaaagu ggcaccgagu cggugc                     106


<210>  11
<211>  116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA2.1 Aff1

<400>  11
ccuucagcuc agugacagug guuuaagagc uaugcuggaa acagcauagc aaguuuaaau       60

aaggcuaguc cguuaucaac uuugcuggaa acagcaaagu ggcaccgagu cggugc          116


<210>  12
<211>  96
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA1 pcsk9

<400>  12
agcagcagcg gcggcaacag guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugc                                 96


<210>  13
<211>  106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA2.0 pcsk9

<400>  13
agcagcagcg gcggcaacag guuuaagagc uaugcuggaa acagcauagc aaguuuaaau       60

aaggcuaguc cguuaucaac uugaaaaagu ggcaccgagu cggugc                     106


<210>  14
<211>  116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA2.1 pcsk9

<400>  14
agcagcagcg gcggcaacag guuuaagagc uaugcuggaa acagcauagc aaguuuaaau       60

aaggcuaguc cguuaucaac uuugcuggaa acagcaaagu ggcaccgagu cggugc          116


<210>  15
<211>  96
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA1 serping1

<400>  15
guuugcaaga cagaggcgaa guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugc                                 96


<210>  16
<211>  106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA2.0 serping1

<400>  16
guuugcaaga cagaggcgaa guuuaagagc uaugcuggaa acagcauagc aaguuuaaau       60

aaggcuaguc cguuaucaac uugaaaaagu ggcaccgagu cggugc                     106


<210>  17
<211>  116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA2.1 serping1

<400>  17
guuugcaaga cagaggcgaa guuuaagagc uaugcuggaa acagcauagc aaguuuaaau       60

aaggcuaguc cguuaucaac uuugcuggaa acagcaaagu ggcaccgagu cggugc          116


<210>  18
<211>  96
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA1 vegfa(site 3)

<400>  18
ggugagugag ugugugcgug guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugc                                 96


<210>  19
<211>  106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA2.0 vegfa(site 3)

<400>  19
ggugagugag ugugugcgug guuuaagagc uaugcuggaa acagcauagc aaguuuaaau       60

aaggcuaguc cguuaucaac uugaaaaagu ggcaccgagu cggugc                     106


<210>  20
<211>  116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA2.1 vegfa(site 3)

<400>  20
ggugagugag ugugugcgug guuuaagagc uaugcuggaa acagcauagc aaguuuaaau       60

aaggcuaguc cguuaucaac uuugcuggaa acagcaaagu ggcaccgagu cggugc          116


<210>  21
<211>  96
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA1 eGFP

<400>  21
cugcuucaug uuagggguag guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugc                                 96


<210>  22
<211>  106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA2.0 eGFP

<400>  22
cugcuucaug uuagggguag guuuaagagc uaugcuggaa acagcauagc aaguuuaaau       60

aaggcuaguc cguuaucaac uugaaaaagu ggcaccgagu cggugc                     106


<210>  23
<211>  116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA2.1 eGFP

<400>  23
cugcuucaug uuagggguag guuuaagagc uaugcuggaa acagcauagc aaguuuaaau       60

aaggcuaguc cguuaucaac uuugcuggaa acagcaaagu ggcaccgagu cggugc          116


<210>  24
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MVA9

<400>  24
gagtgctggc cgaggccatg agcgactata aggaccacga cg                          42


<210>  25
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MVA10

<400>  25
cctgcggccg ctccggaatt ccatgtgtca ctttttcttt tttgcctg                    48


<210>  26
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MVA64

<400>  26
gatccgctag cgctaccggt accaccatgg actataagga c                           41


<210>  27
<211>  71
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MVA65

<400>  27
ctcgagatct gagtccggtc tgcacaatcg gatagttctg gctaaagttc tttttctttt       60

ttgcctggcc g                                                            71


<210>  28
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MVA66

<400>  28
tcactttttt tcaggttgga ccggtcgcca ccatggtg                               38


<210>  29
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MVA67

<400>  29
gctgatcagc gagctctagg cttgtacagc tcgtccatgc c                           41


<210>  30
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MVA111

<400>  30
aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcg             54


<210>  31
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MVA113

<400>  31
ggggcgtact tggcatatga tacacttgat gt                                     32


<210>  32
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MVA114

<400>  32
cttatttaaa cttgctatgc tgtttccagc atagctctta aacaggtct                   49


<210>  33
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MVA115

<400>  33
ggaaacagca tagcaagttt aaataaggct agtccgtta                              39


<210>  34
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MVA141

<400>  34
ccactttgct gtttccagca aagttgataa cggactagcc ttatttaaa                   49


<210>  35
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MVA142

<400>  35
aactttgctg gaaacagcaa agtggcaccg agtcgg                                 36


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AFF1 - Sequence

<400>  36
ccttcagctc agtgacagtg                                                   20


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SERPING1 - Sequence

<400>  37
gtttgcaaga cagaggcgaa                                                   20


<210>  38
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  eGFPmut - Sequence

<400>  38
ctgcttcatg ttaggggtag                                                   20


<210>  39
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VEGFA site 3 - Sequence

<400>  39
ggtgagtgag tgtgtgcgtg                                                   20


<210>  40
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pcsk9 - Sequence

<400>  40
agcagcagcg gcggcaacag                                                   20


<210>  41
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AFF1 - Fw primer

<400>  41
agaggcttgc acttaactgg t                                                 21


<210>  42
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  eGFPmut - Fw primer

<400>  42
attgacgcaa atgggcggta g                                                 21


<210>  43
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VEGFA site 3 - Fw primer

<400>  43
ctcagctcca caaacttggt gcc                                               23


<210>  44
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pcsk9 - Fw primer

<400>  44
tctctctttc tgaggctaga gga                                               23


<210>  45
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AFF1 - Rv primer

<400>  45
aaacactggg ctcgtcagtt                                                   20


<210>  46
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  eGFPmut - Rv primer

<400>  46
ttacttgtac agctcgtcca tgccg                                             25


<210>  47
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VEGFA site 3 - Rv primer

<400>  47
agcccgccgc aatgaagg                                                     18


<210>  48
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pcsk9 - Rv primer

<400>  48
ccccaagtct atttccaagc cg                                                22


<210>  49
<211>  7751
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCCL/PGK-eGFP

<400>  49
aagcttggcc attgcatacg ttgtatccat atcataatat gtacatttat attggctcat       60

gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag taatcaatta      120

cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg      180

gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc      240

ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa      300

ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca      360

atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta      420

cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt      480

acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg      540

acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca      600

actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca      660

gagctcgttt agtgaaccgg ggtctctctg gttagaccag atctgagcct gggagctctc      720

tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt      780

agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc      840

agtgtggaaa atctctagca gtggcgcccg aacagggacc tgaaagcgaa agggaaacca      900

gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg      960

cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc     1020

gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg     1080

ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa     1140

cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga     1200

cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta     1260

gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac     1320

aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt     1380

cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt     1440

agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag     1500

agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag     1560

cactatgggc gcagcctcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat     1620

agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact     1680

cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa     1740

ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt     1800

gccttggaat gctagttgga gtaataaatc tctggaacag attggaatca cacgacctgg     1860

atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa     1920

tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt     1980

ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata     2040

gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt     2100

aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac     2160

aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta     2220

gtgaacggat ctcgacggta tcggttaact tttaaaagaa aaggggggat tggggggtac     2280

agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa     2340

aaacaaatta caaaaattca aaattttatc gatcacgaga ctagcctcga cgatggtcga     2400

gtaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc     2460

gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg     2520

taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct     2580

agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt     2640

agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg     2700

cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg     2760

aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag     2820

gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc     2880

ctcttcctca tctccgggcc tttcgacctc tagcgggatc caccggtcgc caccatggtg     2940

agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac     3000

gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag     3060

ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg     3120

accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac     3180

gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag     3240

gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac     3300

cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg     3360

gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc     3420

aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac     3480

taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg     3540

agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg     3600

gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcggc     3660

ctcgagggaa ttccgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta     3720

ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc     3780

atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt     3840

ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg     3900

ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt     3960

tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct     4020

ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt     4080

cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct     4140

acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc     4200

ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct     4260

ccccgcatcg ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta     4320

gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga     4380

agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg     4440

gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg     4500

cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc     4560

ttttagtcag tgtggaaaat ctctagcagc atctagctag aattaattcc gtgtattcta     4620

tagtgtcacc taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt     4680

tctaacgaca atatgtacaa gcctaattgt gtagcatctg gcttactgaa gcagacccta     4740

tcatctctct cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt ctgagtttct     4800

ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt catcgctagc     4860

ctaggctttt gcgtcgagac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct     4920

cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc     4980

gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc     5040

gcccttccca acagttgcgc agcctgaatg gcgaatggcg cgacgcgccc tgtagcggcg     5100

cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc     5160

tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc     5220

gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg     5280

accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg     5340

tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg     5400

gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt     5460

cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa     5520

tattaacgtt tacaatttcc caggtggcac ttttcgggga aatgtgcgcg gaacccctat     5580

ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata     5640

aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct     5700

tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa     5760

agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa     5820

cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt     5880

taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg     5940

tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca     6000

tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa     6060

cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt     6120

gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc     6180

cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa     6240

actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga     6300

ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc     6360

tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga     6420

tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga     6480

acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga     6540

ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat     6600

ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt     6660

ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct     6720

gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc     6780

ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc     6840

aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc     6900

gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc     6960

gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg     7020

aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata     7080

cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta     7140

tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc     7200

ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg     7260

atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt     7320

cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt     7380

ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga     7440

gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc     7500

cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg     7560

cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca     7620

ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg     7680

aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa     7740

gctggagctg c                                                          7751


<210>  50
<211>  11007
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGFP-PH-gagpol-D64V

<400>  50
gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc      180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt      240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata      300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      420

attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt      480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      780

gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc      840

gctagcgcta ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt      900

gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga      960

gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa     1020

gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag     1080

ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta     1140

cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt     1200

gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga     1260

ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat     1320

catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga     1380

ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc     1440

cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa     1500

cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg     1560

catggacgag ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt     1620

cgacggtacc gcgggcccgg gatccatgga ctcgggccgg gacttcctga ccctgcacgg     1680

cctacaggat gatgaggatc tacaggcgct gctgaagggc agccagctcc tgaaggtgaa     1740

gtccagctca tggaggagag agcgcttcta caagttgcag gaggactgca agaccatctg     1800

gcaggagtcc cgcaaggtca tgcggacccc ggagtcccag ctgttctcca tcgaggacat     1860

tcaggaggtg cgaatggggc accgcacgga gggtctggag aagttcgccc gtgatgtgcc     1920

cgaggaccgc tgcttctcca ttgtcttcaa ggaccagcgc aatacactag acctcatcgc     1980

cccatcgcca gctgatgccc agcactgggt gctggggctg cacaagatca tccaccactc     2040

aggctccatg gaccagcgtc agaagctaca gcactggatt cactcctgct tgcgaaaagc     2100

tgacaaaaac aaggacaaca agatgagctt caaggagctg cagaacttcc tgaaggagct     2160

caacatccag cctcgagccg aattcaccct ggccgccagg gccagcgtgc tgagcggcgg     2220

cgagctggac aggtgggaga agatcaggct gaggcccggc ggcaagaaga agtataagct     2280

gaagcacatc gtgtgggcca gcagggagct ggagaggttc gccgtgaacc ccggcctgct     2340

ggagaccagc gagggctgca ggcagatcct gggccagctg cagcccagcc tgcagaccgg     2400

cagcgaggag ctgaggagcc tgtacaacac cgtggccacc ctgtactgcg tgcaccagag     2460

gatcgagatc aaggacacca aggaggccct ggacaagatc gaggaggagc agaacaagtc     2520

caagaagaag gcccagcagg ccgccgccga caccggccac agcagccagg tgagccagaa     2580

ctaccccatc gtgcagaaca tccagggcca gatggtgcac caggccatca gccccaggac     2640

cctgaacgcc tgggtgaagg tggtggagga gaaggccttc agccccgagg tgatccccat     2700

gttcagcgcc ctgagcgagg gagccacccc ccaggacctg aacaccatgc tgaacaccgt     2760

gggcggccac caggccgcca tgcagatgct gaaggagacc atcaacgagg aggccgccga     2820

gtgggacagg gtgcaccccg tgcacgccgg ccccatcgcc cccggccaga tgagggagcc     2880

ccgcggcagc gacatcgccg gcaccaccag caccctgcag gagcagatcg gctggatgac     2940

caacaacccc cccatccccg tgggcgaaat ctacaagagg tggatcatcc tgggcctgaa     3000

caagatcgtg aggatgtaca gccccaccag catcctggat atcaggcagg gccccaaaga     3060

gcccttcagg gactacgtgg acaggttcta caagaccctg cgcgccgagc aggccagcca     3120

ggaggtgaag aactggatga ccgagaccct gctggtgcag aacgccaacc ccgactgcaa     3180

gaccatcctg aaggccctgg gacccgccgc caccctggag gagatgatga ccgcctgcca     3240

gggcgtgggc ggccccggcc acaaggccag ggtgctggcc gaggccatga gccaggtgac     3300

caacaccgcc accatcatga tgcagagggg caacttcagg aaccagagga agatggtgaa     3360

gtgcttcaac tgcggcaagg agggccacac cgccaggaac tgccgcgccc ccaggaagaa     3420

gggctgctgg aagtgcggca aggagggcca ccagatgaag gactgcaccg agaggcaggc     3480

taatttttta gggaagatct ggccttccta caagggaagg ccagggaatt ttcttcagag     3540

cagaccagag ccaacagccc caccatttct tcagagcaga ccagagccaa cagccccacc     3600

agaagagagc ttcaggtctg gggtagagac aacaactccc cctcagaagc aggagccgat     3660

agacaaggaa ctgtatcctt taacttccct cagatcactc tttggcaacg acccctcgtc     3720

acaataaaga tcggtggcca gctgaaggag gccctgctgg acaccggcgc cgacgacacc     3780

gtgctggagg agatgagcct gcccggcagg tggaagccca agatgatcgg cggcatcggc     3840

ggcttcatca aggtgaggca gtacgaccag atcctgatcg agatctgcgg ccacaaggcc     3900

atcggcaccg tgctggtggg acctacacct gtgaacatca tcggcaggaa cctgctgacc     3960

cagatcggct gcaccctgaa cttccccatc agccccatcg agaccgtgcc cgtgaagctg     4020

aagcccggca tggacggccc taaggtgaag cagtggcccc tgaccgagga gaagatcaag     4080

gccctggtgg agatctgcac cgagatggag aaggagggca agatcagcaa gatcggcccc     4140

gagaacccct acaacacccc cgtgttcgcc atcaagaaga aggacagcac caagtggagg     4200

aagctggtgg acttcaggga gctgaacaag aggacccagg acttctggga ggtgcagctg     4260

ggcatccccc accccgccgg cctgaagaag aagaagagcg tgaccgtgct ggacgtgggc     4320

gacgcctact tcagcgtgcc cctggacgag gacttcagga agtataccgc cttcaccatc     4380

cccagcatca acaacgagac ccccggcatc cgctaccagt acaacgtgct gccccagggc     4440

tggaagggca gccccgccat cttccagagc agcatgacaa agatcctgga gcccttcaag     4500

aagcagaacc ccgacatcgt gatctatcag tacatggacg acctgtacgt gggcagcgac     4560

ctggagatcg gccagcacag gaccaagatc gaggagctga ggcagcacct gctgaggtgg     4620

ggcctgacca cccccgacaa gaagcaccag aaggagcccc cattcctgtg gatgggctac     4680

gagctgcacc ccgacaagtg gaccgtgcag cccatcgtgc tgcccgagaa ggacagctgg     4740

accgtgaacg acattcagaa gctggtgggc aagctgaact gggccagcca gatctacccc     4800

ggcatcaagg tgaggcagct gtgcaagctg ctgaggggca caaaggctct gaccgaggtg     4860

atccccctga ccgaggaggc cgagctggag ctggccgaga acagggagat cctgaaggag     4920

cccgtgcacg gcgtgtacta cgaccccagc aaggacctga tcgccgagat ccagaagcag     4980

ggccagggcc agtggaccta ccagatctac caggagccct tcaagaacct gaagaccggc     5040

aagtacgccc gcatgcgcgg cgcccacacc aacgacgtga agcagctgac cgaggccgtg     5100

cagaagatca ccaccgagag catcgtgatc tggggcaaga ctcctaagtt caagctgccc     5160

atccagaagg agacctggga gacctggtgg accgagtact ggcaggccac ctggattccc     5220

gagtgggagt tcgtgaacac ccctcccctg gtgaagctgt ggtatcagct ggagaaggag     5280

cccatcgtgg gcgccgagac cttctacgtg gacggcgccg ccaacaggga gaccaagctg     5340

ggcaaggccg gctacgtgac caacaagggc cgccagaagg tggtgcccct gaccaacacc     5400

accaaccaga agaccgagct gcaggctatc tacctggccc tgcaggactc aggcctggag     5460

gtgaacatcg tgaccgacag ccagtacgcc ctgggcatca tccaggccca gcccgacaag     5520

agcgagagcg agctggtgaa ccagatcatc gagcagctga tcaagaagga gaaggtgtac     5580

ctggcctggg tgcccgccca caagggcatc ggcggcaacg agcaggtgga caagctggtg     5640

agcgccggca tcaggaagat cctgttcctg gacggcatcg acaaggccca ggacgagcac     5700

gagaagtacc acagcaactg gagggctatg gctagcgact tcaacctgcc tcccgtggtg     5760

gctaaggaga tcgtggccag ctgcgacaag tgccagctga agggcgaggc catgcacggc     5820

caggtggact gcagccccgg catctggcag ctggtttgca cccacctgga gggcaaggtg     5880

atcctggtgg ccgtgcacgt ggcctccggc tacatcgagg ccgaggtgat ccccgccgag     5940

accggccagg agaccgccta cttcctgctg aagctggccg gccgctggcc cgtgaagacc     6000

atccacaccg acaacggcag caacttcacc agcgccaccg tgaaggccgc ctgctggtgg     6060

gccggcatca agcaggagtt cggcatcccc tacaaccccc agtctcaggg cgtggtggag     6120

agcatgaaca aggagctgaa gaagatcatc ggccaggtga gggaccaggc cgagcacctg     6180

aagaccgccg tgcagatggc cgtgttcatc cacaacttca agaggaaggg cggcatcggc     6240

ggctacagcg ccggcgagag gatcgtggac atcatcgcca ccgacatcca gaccaaggag     6300

ctgcagaagc agatcaccaa gatccagaac ttcagggtgt actacaggga cagcaggaac     6360

cctctgtgga agggccccgc caagctgctg tggaagggcg agggcgccgt ggtgatccag     6420

gacaacagcg acatcaaggt ggtgcccagg aggaaggcca agatcatcag ggactacggc     6480

aagcagatgg ccggcgacga ctgcgtggcc tccaggcagg acgaggactg actcgagcat     6540

gcatctagag ggccctattc tatagtgtca cctaaatgct agagctcgct gatcagcctc     6600

gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac     6660

cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg     6720

tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga     6780

ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga     6840

aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc     6900

ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcgcgc     6960

tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct     7020

aaatcggggc atccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa     7080

acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc     7140

tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact     7200

caaccctatc tcggtctatt cttttgattt ataagggatt ttggggattt cggcctattg     7260

gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt     7320

cagttagggt gtggaaagtc cccaggctcc ccaggcaggc agaagtatgc aaagcatgca     7380

tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat     7440

gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc     7500

gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat     7560

ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt     7620

ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc     7680

tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg     7740

ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg     7800

ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa     7860

gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct     7920

ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga     7980

ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc     8040

cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac     8100

ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc     8160

cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact     8220

gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga     8280

tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg     8340

ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga     8400

agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga     8460

ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg     8520

ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc     8580

gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc     8640

cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat     8700

aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg     8760

cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg     8820

acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat     8880

ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc     8940

taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga     9000

aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt     9060

attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg     9120

cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac     9180

gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg     9240

ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca     9300

agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc     9360

tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc     9420

ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag     9480

gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc     9540

ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca     9600

gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg     9660

aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg     9720

aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct     9780

ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa     9840

gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa     9900

gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa     9960

tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc    10020

ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga    10080

ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca    10140

atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc    10200

ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat    10260

tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc    10320

attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt    10380

tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc    10440

ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg    10500

gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt    10560

gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg    10620

gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga    10680

aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg    10740

taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg    10800

tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt    10860

tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc    10920

atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca    10980

tttccccgaa aagtgccacc tgacgtc                                        11007


<210>  51
<211>  10183
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pLentiGuide-puro

<400>  51
taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt       60

taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat      120

tatcgtttca gacccacctc ccaaccccga ggggacccag agagggccta tttcccatga      180

ttccttcata tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga      240

ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt      300

agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa      360

agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc ggagacggtt      420

gtaaatgagc acacaaaata cacatgctaa aatattatat tctatgacct ttataaaatc      480

aaccaaaatc ttctttttaa taactttagt atcaataatt agaattttta tgttcctttt      540

tgcaaacttt taataaaaat gagcaaaata aaaaaacgct agttttagta actcgcgttg      600

ttttcttcac ctttaataat agctactcca ccacttgttc ctaagcggtc agctcctgct      660

tcaatcattt tttgagcatc ttcaaatgtt ctaactccac cagctgcttt aactaaagca      720

ttgtctttaa caactgactt cattagttta acatcttcaa atgttgcacc tgattttgaa      780

aatcctgttg atgttttaac aaattctaat ccagcttcaa cagctatttc acaagctttc      840

atgatttctt cttttgttaa taaacaattt tccataatac atttaacaac atgtgatcca      900

gctgcttttt ttacagcttt catgtcttct aaaactaatt cataattttt gtcttttaat      960

gcaccaatat ttaataccat atcaatttct gttgcaccat ctttaattgc ttcagaaact     1020

tcgaatgctt ttgtagctgt tgtgcatgca cctagaggaa aacctacaac atttgttatt     1080

cctacatttg tgccttttaa taattcttta caatagcttg ttcaatatga attaacacaa     1140

actgttgcaa aatcaaattc aattgcttca tcacataatt gtttaatttc agctttcgta     1200

gcatcttgtt ttaataatgt gtgatctata tatttgttta gtttcatttt ttctcctata     1260

tattcatttt taattttaat tctttaataa tttcgtctac tttaacttta gcgttttgaa     1320

cagattcacc aacacctata aaataaattt ttagtttagg ttcagttcca cttgggcgaa     1380

cagcaaatca tgacttatct tctaaataaa attttagtaa gtcttgtcct ggcatattat     1440

acattccatc gatgtagtct tcaacattaa caactttaag tccagcaatt tgagttaagg     1500

gtgttgctct caatgatttc attaatggtt caatttttaa tttcttttct tctggtttaa     1560

aattcaagtt taaagtgaaa gtgtaatatg cacccatttc tttaaataaa tcttctaaat     1620

agtctactaa tgttttattt tgttttttat aaaatcaagc agcctctgct attaatatag     1680

aagcttgtat tccatcttta tctctagctg agtcatcaat tacatatcca taactttctt     1740

cataagcaaa aacaaaattt aatccgttat cttcttcttt agcaatttct ctacccattc     1800

atttaaatcc agttaaagtt tttacaatat taactccata tttttcatga gcgattctat     1860

cacccaaatc acttgttaca aaacttgaat atagagccgg attttttgga atgctattta     1920

agcgttttag atttgataat tttcaatcaa ttaaaattgg tcctgtttga tttccatcta     1980

atcttacaaa atgaccatca tgttttattg ccattccaaa tctgtcagca tctgggtcat     2040

tcataataat aatatctgca tcatgtttaa taccatattc aagcggtatt tttcatgcag     2100

gatcaaattc tggatttgga tttacaacat ttttaaatgt ttcatcttca aatgcatgct     2160

cttcaacctc aataacgtta tatcctgatt cacgtaatat ttttggggta aatttagttc     2220

ctgttccatt aactgcgcta aaaataattt ttaaatcttt tttagcttct tgctcttttt     2280

tgtacgtctc tgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa     2340

cttgaaaaag tggcaccgag tcggtgcttt tttaagcttg gcgtaactag atcttgagac     2400

aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag     2460

gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa     2520

ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc     2580

ggctcgaggg ggcccgggtg caaagatgga taaagtttta aacagagagg aatctttgca     2640

gctaatggac cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg     2700

cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgatccg     2760

gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc     2820

tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt     2880

ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg     2940

gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg     3000

tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt     3060

aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg     3120

tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt     3180

aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg     3240

gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg     3300

cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac     3360

gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg     3420

ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc     3480

cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg     3540

cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg     3600

actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta     3660

cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg     3720

tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt     3780

ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc     3840

catttcaggt gtcgtgacgt acggccacca tgaccgagta caagcccacg gtgcgcctcg     3900

ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc     3960

ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag     4020

aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg     4080

ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga     4140

tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag     4200

gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggagtct     4260

cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg     4320

ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct     4380

acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct     4440

ggtgcatgac ccgcaagccc ggtgcctgaa cgcgttaagt cgacaatcaa cctctggatt     4500

acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg     4560

gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct     4620

cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc     4680

aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca     4740

ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac     4800

tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt     4860

ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct     4920

ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc     4980

cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga     5040

cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa     5100

ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca     5160

ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat     5220

ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt     5280

gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc     5340

cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca     5400

tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt     5460

gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa     5520

agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca     5580

tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt     5640

tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc     5700

gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg     5760

tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac     5820

gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt     5880

tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca     5940

gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg     6000

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct     6060

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc     6120

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg     6180

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt     6240

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg     6300

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc     6360

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg     6420

cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa     6480

tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa     6540

gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct     6600

tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg     6660

tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg     6720

ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt     6780

atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga     6840

cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga     6900

attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac     6960

gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg     7020

ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac     7080

gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct     7140

agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct     7200

gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg     7260

gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat     7320

ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg     7380

tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat     7440

tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct     7500

catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa     7560

gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa     7620

aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc     7680

gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta     7740

gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct     7800

gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg     7860

atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag     7920

cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc     7980

cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg     8040

agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt     8100

tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg     8160

gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca     8220

catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg     8280

agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc     8340

ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag     8400

ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag     8460

ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg     8520

tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa     8580

gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct     8640

tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa     8700

ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta     8760

ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc     8820

agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag     8880

atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc     8940

ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga     9000

tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact     9060

tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc     9120

gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag     9180

gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc     9240

gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag     9300

tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag     9360

aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac     9420

ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa     9480

aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg     9540

cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga     9600

agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag     9660

gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt     9720

gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag     9780

gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag     9840

gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc     9900

ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga     9960

aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa    10020

cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc    10080

ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta    10140

ttggaattag ataaatgggc aagtttgtgg aattggttta aca                      10183


<210>  52
<211>  13191
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMDLg/p-RRE-D64V-PCS-SpCas9

<400>  52
ggatcccctg agggggcccc catgggctag aggatccggc ctcggcctct gcataaataa       60

aaaaaattag tcagccatga gcttggccca ttgcatacgt tgtatccata tcataatatg      120

tacatttata ttggctcatg tccaacatta ccgccatgtt gacattgatt attgactagt      180

tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt      240

acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg      300

tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg      360

gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt      420

acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg      480

accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg      540

gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt      600

ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac      660

tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg      720

tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat      780

ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccc ctcgaagctt      840

acatgtggta ccgagctcgg atcctgagaa cttcagggtg agtctatggg acccttgatg      900

ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag      960

ggtacacata ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct     1020

tcttttaata tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc     1080

agggcaataa tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat     1140

aatttctggg ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg     1200

taactgatgt aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct     1260

tttattttat ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta     1320

atcatgttca tacctcttat cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg     1380

ctggcccatc actttggcaa agcacgtgag atctgaattc gagatctgcc gccgccatgg     1440

gtgcgagagc gtcagtatta agcgggggag aattagatcg atgggaaaaa attcggttaa     1500

ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag     1560

aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg     1620

gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag     1680

tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag     1740

acaagataga ggaagagcaa aacaaaagta agaaaaaagc acagcaagca gcagctgaca     1800

caggacacag caatcaggtc agccaaaatt accctatagt gcagaacatc caggggcaaa     1860

tggtacatca ggccatatca cctagaactt taaatgcatg ggtaaaagta gtagaagaga     1920

aggctttcag cccagaagtg atacccatgt tttcagcatt atcagaagga gccaccccac     1980

aagatttaaa caccatgcta aacacagtgg ggggacatca agcagccatg caaatgttaa     2040

aagagaccat caatgaggaa gctgcagaat gggatagagt gcatccagtg catgcagggc     2100

ctattgcacc aggccagatg agagaaccaa ggggaagtga catagcagga actactagta     2160

ctagtaccct tcaggaacaa ataggatgga tgacacataa tccacctatc ccagtaggag     2220

aaatctataa aagatggata atcctgggat taaataaaat agtaagaatg tatagcccta     2280

ccagcattct ggacataaga caaggaccaa aggaaccctt tagagactat gtagaccgat     2340

tctataaaac tctaagagcc gagcaagctt cacaagaggt aaaaaattgg atgacagaaa     2400

ccttgttggt ccaaaatgcg aacccagatt gtaagactat tttaaaagca ttgggaccag     2460

gagcgacact agaagaaatg atgacagcat gtcagggagt ggggggaccc ggccataaag     2520

caagagtttt ggctgaagca atgagccaag taacaaatcc agctaccata atgatacaga     2580

aaggcaattt taggaaccaa agaaagactg ttaagtgttt caattgtggc aaagaagggc     2640

acatagccaa aaattgcagg gcccctagga aaaagggctg ttggaaatgt ggaaaggaag     2700

gacaccaaat gaaagattgt actgagagac aggctaattt tttagggaag atctggcctt     2760

cccacaaggg aaggccaggg aattttcttc agagcagacc agagccaaca gccccaccag     2820

aagagagctt caggtttggg gaagagacaa caactccctc tcagaagcag gagccgatag     2880

acaaggaact gtatccttta gcttccctca gatcactctt tggcagcgac ccctcgtcac     2940

aataaagata ggggggcaat taaaggaagc tctattagat acaggagcag atgatacagt     3000

attagaagaa atgaatttgc caggaagatg gaaaccaaaa atgatagggg gaattggagg     3060

ttttatcaaa gtaggacagt atgatcagat actcatagaa atctgtggac ataaagctat     3120

aggtacagta ttagtaggac ctacacctgt caacataatt ggaagaaatc tgttgactca     3180

gattggttgc actttaaatt ttcccattag ccctattgag actgtaccag taaaattaaa     3240

gccaggaatg gatggcccaa aagttaaaca atggccattg acagaagaaa aaataaaagc     3300

attagtagaa atttgtacag agatggaaaa ggaagggaaa atttcaaaaa ttgggcctga     3360

aaatccatac aatactccag tatttgccat aaagaaaaaa gacagtacta aatggagaaa     3420

attagtagat ttcagagaac ttaataagag aactcaagac ttctgggaag ttcaattagg     3480

aataccacat cccgcagggt taaaaaagaa aaaatcagta acagtactgg atgtgggtga     3540

tgcatatttt tcagttccct tagatgaaga cttcaggaag tatactgcat ttaccatacc     3600

tagtataaac aatgagacac cagggattag atatcagtac aatgtgcttc cacagggatg     3660

gaaaggatca ccagcaatat tccaaagtag catgacaaaa atcttagagc cttttagaaa     3720

acaaaatcca gacatagtta tctatcaata catggatgat ttgtatgtag gatctgactt     3780

agaaataggg cagcatagaa caaaaataga ggagctgaga caacatctgt tgaggtgggg     3840

acttaccaca ccagacaaaa aacatcagaa agaacctcca ttcctttgga tgggttatga     3900

actccatcct gataaatgga cagtacagcc tatagtgctg ccagaaaaag acagctggac     3960

tgtcaatgac atacagaagt tagtggggaa attgaattgg gcaagtcaga tttacccagg     4020

gattaaagta aggcaattat gtaaactcct tagaggaacc aaagcactaa cagaagtaat     4080

accactaaca gaagaagcag agctagaact ggcagaaaac agagagattc taaaagaacc     4140

agtacatgga gtgtattatg acccatcaaa agacttaata gcagaaatac agaagcaggg     4200

gcaaggccaa tggacatatc aaatttatca agagccattt aaaaatctga aaacaggaaa     4260

atatgcaaga atgaggggtg cccacactaa tgatgtaaaa caattaacag aggcagtgca     4320

aaaaataacc acagaaagca tagtaatatg gggaaagact cctaaattta aactgcccat     4380

acaaaaggaa acatgggaaa catggtggac agagtattgg caagccacct ggattcctga     4440

gtgggagttt gttaataccc ctcccttagt gaaattatgg taccagttag agaaagaacc     4500

catagtagga gcagaaacct tctatgtaga tggggcagct aacagggaga ctaaattagg     4560

aaaagcagga tatgttacta atagaggaag acaaaaagtt gtcaccctaa ctgacacaac     4620

aaatcagaag actgagttac aagcaattta tctagctttg caggattcgg gattagaagt     4680

aaacatagta acagactcac aatatgcatt aggaatcatt caagcacaac cagatcaaag     4740

tgaatcagag ttagtcaatc aaataataga gcagttaata aaaaaggaaa aggtctatct     4800

ggcatgggta ccagcacaca aaggaattgg aggaaatgaa caagtagata aattagtcag     4860

tgctggaatc aggaaagtac tatttttaga tggaatagat aaggcccaag atgaacatga     4920

gaaatatcac agtaattgga gagcaatggc tagtgatttt aacctgccac ctgtagtagc     4980

aaaagaaata gtagccagct gtgataaatg tcagctaaaa ggagaagcca tgcatggaca     5040

agtagactgt agtccaggaa tatggcaact agtttgtaca catttagaag gaaaagttat     5100

cctggtagca gttcatgtag ccagtggata tatagaagca gaagttattc cagcagaaac     5160

agggcaggaa acagcatatt ttcttttaaa attagcagga agatggccag taaaaacaat     5220

acatactgac aatggcagca atttcaccgg tgctacggtt agggccgcct gttggtgggc     5280

agggatcaag caggaatttg gcattcccta caatccccaa agccaaggag tagtagaatc     5340

tatgaataat gaattaaaga aaattatagg acaggtaaga gatcaggctg aacaccttaa     5400

gacagcagta caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg     5460

gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt     5520

acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc     5580

agtttggaaa ggaccagcaa agctcctctg gaaaggtgaa ggggcagtag taatacaaga     5640

taatagtgac ataaaagtag tgccaagaag aaaagcaaag atcatcaggg attatggaaa     5700

acagatggca ggtgatgatt gtgtggcaag tagacaggat gaggataagg ccagagtgct     5760

ggccgaggcc atgagcgact ataaggacca cgacggagac tacaaggatc atgatattga     5820

ttacaaagac gatgacgata agatggcccc aaagaagaag cggaaggtcg gtatccacgg     5880

agtcccagca gccgacaaga agtacagcat cggcctggac atcggcacca actctgtggg     5940

ctgggccgtg atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa     6000

caccgaccgg cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga     6060

aacagccgag gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa     6120

ccggatctgc tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt     6180

cttccacaga ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc     6240

catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca     6300

cctgagaaag aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc     6360

cctggcccac atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga     6420

caacagcgac gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga     6480

ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag     6540

caagagcaga cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct     6600

gttcggaaac ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga     6660

cctggccgag gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa     6720

cctgctggcc cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc     6780

cgacgccatc ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct     6840

gagcgcctct atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc     6900

tctcgtgcgg cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa     6960

cggctacgcc ggctacattg acggcggagc cagccaggaa gagttctaca agttcatcaa     7020

gcccatcctg gaaaagatgg acggcaccga ggaactgctc gtgaagctga acagagagga     7080

cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg     7140

agagctgcac gccattctgc ggcggcagga agatttttac ccattcctga aggacaaccg     7200

ggaaaagatc gagaagatcc tgaccttccg catcccctac tacgtgggcc ctctggccag     7260

gggaaacagc agattcgcct ggatgaccag aaagagcgag gaaaccatca ccccctggaa     7320

cttcgaggaa gtggtggaca agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa     7380

cttcgataag aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta     7440

cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc     7500

cgccttcctg agcggcgagc agaaaaaggc catcgtggac ctgctgttca agaccaaccg     7560

gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt gcttcgactc     7620

cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct     7680

gctgaaaatt atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga     7740

agatatcgtg ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa     7800

aacctatgcc cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac     7860

cggctggggc aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa     7920

gacaatcctg gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat     7980

ccacgacgac agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggccaggg     8040

cgatagcctg cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat     8100

cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga     8160

gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag     8220

ccgcgagaga atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa     8280

agaacacccc gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca     8340

gaatgggcgg gatatgtacg tggaccagga actggacatc aaccggctgt ccgactacga     8400

tgtggaccat atcgtgcctc agagctttct gaaggacgac tccatcgaca acaaggtgct     8460

gaccagaagc gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa     8520

gaagatgaag aactactggc ggcagctgct gaacgccaag ctgattaccc agagaaagtt     8580

cgacaatctg accaaggccg agagaggcgg cctgagcgaa ctggataagg ccggcttcat     8640

caagagacag ctggtggaaa cccggcagat cacaaagcac gtggcacaga tcctggactc     8700

ccggatgaac actaagtacg acgagaatga caagctgatc cgggaagtga aagtgatcac     8760

cctgaagtcc aagctggtgt ccgatttccg gaaggatttc cagttttaca aagtgcgcga     8820

gatcaacaac taccaccacg cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct     8880

gatcaaaaag taccctaagc tggaaagcga gttcgtgtac ggcgactaca aggtgtacga     8940

cgtgcggaag atgatcgcca agagcgagca ggaaatcggc aaggctaccg ccaagtactt     9000

cttctacagc aacatcatga actttttcaa gaccgagatt accctggcca acggcgagat     9060

ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt gggataaggg     9120

ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa     9180

gaccgaggtg cagacaggcg gcttcagcaa agagtctatc ctgcccaaga ggaacagcga     9240

taagctgatc gccagaaaga aggactggga ccctaagaag tacggcggct tcgacagccc     9300

caccgtggcc tattctgtgc tggtggtggc caaagtggaa aagggcaagt ccaagaaact     9360

gaagagtgtg aaagagctgc tggggatcac catcatggaa agaagcagct tcgagaagaa     9420

tcccatcgac tttctggaag ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa     9480

gctgcctaag tactccctgt tcgagctgga aaacggccgg aagagaatgc tggcctctgc     9540

cggcgaactg cagaagggaa acgaactggc cctgccctcc aaatatgtga acttcctgta     9600

cctggccagc cactatgaga agctgaaggg ctcccccgag gataatgagc agaaacagct     9660

gtttgtggaa cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttctc     9720

caagagagtg atcctggccg acgctaatct ggacaaagtg ctgtccgcct acaacaagca     9780

ccgggataag cccatcagag agcaggccga gaatatcatc cacctgttta ccctgaccaa     9840

tctgggagcc cctgccgcct tcaagtactt tgacaccacc atcgaccgga agaggtacac     9900

cagcaccaaa gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga     9960

gacacggatc gacctgtctc agctgggagg cgacaaaagg ccggcggcca cgaaaaaggc    10020

cggccaggca aaaaagaaaa agtgacacat ggaattccgg agcggccgca ggagctttgt    10080

tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg    10140

tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta    10200

ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa    10260

gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct    10320

ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc    10380

tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca    10440

caagcttccg cggaattcac cccaccagtg caggctgcct atcagaaagt ggtggctggt    10500

gtggctaatg ccctggccca caagtttcac taagctcgct tccttgctgt ccaatttcta    10560

ttaaaggttc cttggttccc taagtccaac tactaaactg ggggatatta tgaagggcct    10620

tgagcatctg gattctgcct aataaaaaac atttattttc attgcaatga tgtatttaaa    10680

ttatttctga atattttact aaaaagggaa tgtgggaggt cagtgcattt aaaacataaa    10740

gaaatgaaga gctagttcaa accttgggaa aatacactat atcttaaact ccatgaaaga    10800

aggtgaggct gcaaacagct aatgcacatt ggcaacagcc ctgatgccta tgccttattc    10860

atccctcaga aaaggattca agtagaggct tgatttggag gttaaagttt ggctatgctg    10920

tattttacat tacttattgt tttagctgtc ctcatgaatg tcttttcact acccatttgc    10980

ttatcctgca tctctcagcc ttgactccac tcagttctct tgcttagaga taccaccttt    11040

cccctgaagt gttccttcca tgttttacgg cgagatggtt tctcctcgcc tggccactca    11100

gccttagttg tctctgttgt cttatagagg tctacttgaa gaaggaaaaa cagggggcat    11160

ggtttgactg tcctgtgagc ccttcttccc tgcctccccc actcacagtg acccggaatc    11220

cctcgacatg gcagtctagc actagtgcgg ccgcagatct gcttcctcgc tcactgactc    11280

gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg    11340

gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa    11400

ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga    11460

cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag    11520

ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct    11580

taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg    11640

ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc    11700

ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt    11760

aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta    11820

tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac    11880

agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc    11940

ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat    12000

tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc    12060

tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt    12120

cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta    12180

aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct    12240

atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg    12300

cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga    12360

tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt    12420

atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt    12480

taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt    12540

tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat    12600

gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc    12660

cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc    12720

cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat    12780

gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag    12840

aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt    12900

accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc    12960

ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa    13020

gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg    13080

aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa    13140

taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg t             13191


<210>  53
<211>  10707
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMDLg/p-PCS-hyPBase

<400>  53
ggatcccctg agggggcccc catgggctag aggatccggc ctcggcctct gcataaataa       60

aaaaaattag tcagccatga gcttggccca ttgcatacgt tgtatccata tcataatatg      120

tacatttata ttggctcatg tccaacatta ccgccatgtt gacattgatt attgactagt      180

tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt      240

acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg      300

tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg      360

gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt      420

acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg      480

accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg      540

gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt      600

ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac      660

tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg      720

tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat      780

ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccc ctcgaagctt      840

acatgtggta ccgagctcgg atcctgagaa cttcagggtg agtctatggg acccttgatg      900

ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag      960

ggtacacata ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct     1020

tcttttaata tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc     1080

agggcaataa tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat     1140

aatttctggg ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg     1200

taactgatgt aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct     1260

tttattttat ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta     1320

atcatgttca tacctcttat cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg     1380

ctggcccatc actttggcaa agcacgtgag atctgaattc gagatctgcc gccgccatgg     1440

gtgcgagagc gtcagtatta agcgggggag aattagatcg atgggaaaaa attcggttaa     1500

ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag     1560

aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg     1620

gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag     1680

tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag     1740

acaagataga ggaagagcaa aacaaaagta agaaaaaagc acagcaagca gcagctgaca     1800

caggacacag caatcaggtc agccaaaatt accctatagt gcagaacatc caggggcaaa     1860

tggtacatca ggccatatca cctagaactt taaatgcatg ggtaaaagta gtagaagaga     1920

aggctttcag cccagaagtg atacccatgt tttcagcatt atcagaagga gccaccccac     1980

aagatttaaa caccatgcta aacacagtgg ggggacatca agcagccatg caaatgttaa     2040

aagagaccat caatgaggaa gctgcagaat gggatagagt gcatccagtg catgcagggc     2100

ctattgcacc aggccagatg agagaaccaa ggggaagtga catagcagga actactagta     2160

ctagtaccct tcaggaacaa ataggatgga tgacacataa tccacctatc ccagtaggag     2220

aaatctataa aagatggata atcctgggat taaataaaat agtaagaatg tatagcccta     2280

ccagcattct ggacataaga caaggaccaa aggaaccctt tagagactat gtagaccgat     2340

tctataaaac tctaagagcc gagcaagctt cacaagaggt aaaaaattgg atgacagaaa     2400

ccttgttggt ccaaaatgcg aacccagatt gtaagactat tttaaaagca ttgggaccag     2460

gagcgacact agaagaaatg atgacagcat gtcagggagt ggggggaccc ggccataaag     2520

caagagtttt ggctgaagca atgagccaag taacaaatcc agctaccata atgatacaga     2580

aaggcaattt taggaaccaa agaaagactg ttaagtgttt caattgtggc aaagaagggc     2640

acatagccaa aaattgcagg gcccctagga aaaagggctg ttggaaatgt ggaaaggaag     2700

gacaccaaat gaaagattgt actgagagac aggctaattt tttagggaag atctggcctt     2760

cccacaaggg aaggccaggg aattttcttc agagcagacc agagccaaca gccccaccag     2820

aagagagctt caggtttggg gaagagacaa caactccctc tcagaagcag gagccgatag     2880

acaaggaact gtatccttta gcttccctca gatcactctt tggcagcgac ccctcgtcac     2940

aataaagata ggggggcaat taaaggaagc tctattagat acaggagcag atgatacagt     3000

attagaagaa atgaatttgc caggaagatg gaaaccaaaa atgatagggg gaattggagg     3060

ttttatcaaa gtaggacagt atgatcagat actcatagaa atctgtggac ataaagctat     3120

aggtacagta ttagtaggac ctacacctgt caacataatt ggaagaaatc tgttgactca     3180

gattggttgc actttaaatt ttcccattag ccctattgag actgtaccag taaaattaaa     3240

gccaggaatg gatggcccaa aagttaaaca atggccattg acagaagaaa aaataaaagc     3300

attagtagaa atttgtacag agatggaaaa ggaagggaaa atttcaaaaa ttgggcctga     3360

aaatccatac aatactccag tatttgccat aaagaaaaaa gacagtacta aatggagaaa     3420

attagtagat ttcagagaac ttaataagag aactcaagac ttctgggaag ttcaattagg     3480

aataccacat cccgcagggt taaaaaagaa aaaatcagta acagtactgg atgtgggtga     3540

tgcatatttt tcagttccct tagatgaaga cttcaggaag tatactgcat ttaccatacc     3600

tagtataaac aatgagacac cagggattag atatcagtac aatgtgcttc cacagggatg     3660

gaaaggatca ccagcaatat tccaaagtag catgacaaaa atcttagagc cttttagaaa     3720

acaaaatcca gacatagtta tctatcaata catggatgat ttgtatgtag gatctgactt     3780

agaaataggg cagcatagaa caaaaataga ggagctgaga caacatctgt tgaggtgggg     3840

acttaccaca ccagacaaaa aacatcagaa agaacctcca ttcctttgga tgggttatga     3900

actccatcct gataaatgga cagtacagcc tatagtgctg ccagaaaaag acagctggac     3960

tgtcaatgac atacagaagt tagtggggaa attgaattgg gcaagtcaga tttacccagg     4020

gattaaagta aggcaattat gtaaactcct tagaggaacc aaagcactaa cagaagtaat     4080

accactaaca gaagaagcag agctagaact ggcagaaaac agagagattc taaaagaacc     4140

agtacatgga gtgtattatg acccatcaaa agacttaata gcagaaatac agaagcaggg     4200

gcaaggccaa tggacatatc aaatttatca agagccattt aaaaatctga aaacaggaaa     4260

atatgcaaga atgaggggtg cccacactaa tgatgtaaaa caattaacag aggcagtgca     4320

aaaaataacc acagaaagca tagtaatatg gggaaagact cctaaattta aactgcccat     4380

acaaaaggaa acatgggaaa catggtggac agagtattgg caagccacct ggattcctga     4440

gtgggagttt gttaataccc ctcccttagt gaaattatgg taccagttag agaaagaacc     4500

catagtagga gcagaaacct tctatgtaga tggggcagct aacagggaga ctaaattagg     4560

aaaagcagga tatgttacta atagaggaag acaaaaagtt gtcaccctaa ctgacacaac     4620

aaatcagaag actgagttac aagcaattta tctagctttg caggattcgg gattagaagt     4680

aaacatagta acagactcac aatatgcatt aggaatcatt caagcacaac cagatcaaag     4740

tgaatcagag ttagtcaatc aaataataga gcagttaata aaaaaggaaa aggtctatct     4800

ggcatgggta ccagcacaca aaggaattgg aggaaatgaa caagtagata aattagtcag     4860

tgctggaatc aggaaagtac tatttttaga tggaatagat aaggcccaag atgaacatga     4920

gaaatatcac agtaattgga gagcaatggc tagtgatttt aacctgccac ctgtagtagc     4980

aaaagaaata gtagccagct gtgataaatg tcagctaaaa ggagaagcca tgcatggaca     5040

agtagactgt agtccaggaa tatggcaact agtttgtaca catttagaag gaaaagttat     5100

cctggtagca gttcatgtag ccagtggata tatagaagca gaagttattc cagcagagac     5160

agggcaggaa acagcatatt ttctcttaaa attagcagga agatggccag taaaaacaat     5220

acatacagac aatggcagca atttcaccag tactacggtt aaggccgcct gttggtgggc     5280

agggatcaag caggaatttg gcattcccta caatccccaa agccaaggag tagtagaatc     5340

tatgaataat gaattaaaga aaattatagg acaggtaaga gatcaggctg aacaccttaa     5400

gacagcagta caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg     5460

gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt     5520

acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc     5580

agtttggaaa ggaccagcaa agctcctctg gaaaggtgaa ggggcagtag taatacaaga     5640

taatagtgac ataaaagtag tgccaagaag aaaagcaaag atcatcaggg attatggaaa     5700

acagatggca ggtgatgatt gtgtggcaag tagacaggat gaggataagg ccagagtgct     5760

ggccgaggcc atgagcatgg gcagcagcct ggacgacgag cacatcctga gcgccctgct     5820

gcagagcgac gacgagctgg tcggcgagga cagcgacagc gaggtgagcg accacgtgag     5880

cgaggacgac gtgcagtccg acaccgagga ggccttcatc gacgaggtgc acgaggtgca     5940

gcctaccagc agcggctccg agatcctgga cgagcagaac gtgatcgagc agcccggcag     6000

ctccctggcc agcaacagga tcctgaccct gccccagagg accatcaggg gcaagaacaa     6060

gcactgctgg tccacctcca agcccaccag gcggagcagg gtgtccgccc tgaacatcgt     6120

gagaagccag aggggcccca ccaggatgtg caggaacatc tacgaccccc tgctgtgctt     6180

caagctgttc ttcaccgacg agatcatcag cgagatcgtg aagtggacca acgccgagat     6240

cagcctgaag aggcgggaga gcatgacctc cgccaccttc agggacacca acgaggacga     6300

gatctacgcc ttcttcggca tcctggtgat gaccgccgtg aggaaggaca accacatgag     6360

caccgacgac ctgttcgaca gatccctgag catggtgtac gtgagcgtga tgagcaggga     6420

cagattcgac ttcctgatca gatgcctgag gatggacgac aagagcatca ggcccaccct     6480

gcgggagaac gacgtgttca cccccgtgag aaagatctgg gacctgttca tccaccagtg     6540

catccagaac tacacccctg gcgcccacct gaccatcgac gagcagctgc tgggcttcag     6600

gggcaggtgc cccttcaggg tctatatccc caacaagccc agcaagtacg gcatcaagat     6660

cctgatgatg tgcgacagcg gcaccaagta catgatcaac ggcatgccct acctgggcag     6720

gggcacccag accaacggcg tgcccctggg cgagtactac gtgaaggagc tgtccaagcc     6780

cgtccacggc agctgcagaa acatcacctg cgacaactgg ttcaccagca tccccctggc     6840

caagaacctg ctgcaggagc cctacaagct gaccatcgtg ggcaccgtga gaagcaacaa     6900

gagagagatc cccgaggtcc tgaagaacag caggtccagg cccgtgggca ccagcatgtt     6960

ctgcttcgac ggccccctga ccctggtgtc ctacaagccc aagcccgcca agatggtgta     7020

cctgctgtcc agctgcgacg aggacgccag catcaacgag agcaccggca agccccagat     7080

ggtgatgtac tacaaccaga ccaagggcgg cgtggacacc ctggaccaga tgtgcagcgt     7140

gatgacctgc agcagaaaga ccaacaggtg gcccatggcc ctgctgtacg gcatgatcaa     7200

catcgcctgc atcaacagct tcatcatcta cagccacaac gtgagcagca agggcgagaa     7260

ggtgcagagc cggaaaaagt tcatgcggaa cctgtacatg ggcctgacct ccagcttcat     7320

gaggaagagg ctggaggccc ccaccctgaa gagatacctg agggacaaca tcagcaacat     7380

cctgcccaaa gaggtgcccg gcaccagcga cgacagcacc gaggagcccg tgatgaagaa     7440

gaggacctac tgcacctact gtcccagcaa gatcagaaga aaggccagcg ccagctgcaa     7500

gaagtgtaag aaggtcatct gccgggagca caacatcgac atgtgccaga gctgtttctg     7560

acacatggaa ttccggagcg gccgcaggag ctttgttcct tgggttcttg ggagcagcag     7620

gaagcactat gggcgcagcc tcaatgacgc tgacggtaca ggccagacaa ttattgtctg     7680

gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc     7740

aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc     7800

taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg     7860

ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga     7920

cctggatgga gtgggacaga gaaattaaca attacacaag cttccgcgga attcacccca     7980

ccagtgcagg ctgcctatca gaaagtggtg gctggtgtgg ctaatgccct ggcccacaag     8040

tttcactaag ctcgcttcct tgctgtccaa tttctattaa aggttccttg gttccctaag     8100

tccaactact aaactggggg atattatgaa gggccttgag catctggatt ctgcctaata     8160

aaaaacattt attttcattg caatgatgta tttaaattat ttctgaatat tttactaaaa     8220

agggaatgtg ggaggtcagt gcatttaaaa cataaagaaa tgaagagcta gttcaaacct     8280

tgggaaaata cactatatct taaactccat gaaagaaggt gaggctgcaa acagctaatg     8340

cacattggca acagccctga tgcctatgcc ttattcatcc ctcagaaaag gattcaagta     8400

gaggcttgat ttggaggtta aagtttggct atgctgtatt ttacattact tattgtttta     8460

gctgtcctca tgaatgtctt ttcactaccc atttgcttat cctgcatctc tcagccttga     8520

ctccactcag ttctcttgct tagagatacc acctttcccc tgaagtgttc cttccatgtt     8580

ttacggcgag atggtttctc ctcgcctggc cactcagcct tagttgtctc tgttgtctta     8640

tagaggtcta cttgaagaag gaaaaacagg gggcatggtt tgactgtcct gtgagccctt     8700

cttccctgcc tcccccactc acagtgaccc ggaatccctc gacatggcag tctagcacta     8760

gtgcggccgc agatctgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg     8820

gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa     8880

cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc     8940

gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc     9000

aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag     9060

ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct     9120

cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta     9180

ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc     9240

cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc     9300

agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt     9360

gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct     9420

gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc     9480

tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca     9540

agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta     9600

agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa     9660

atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg     9720

cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg     9780

actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc     9840

aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc     9900

cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa     9960

ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc    10020

cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg    10080

ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc    10140

cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat    10200

ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg    10260

tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc    10320

ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg    10380

aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat    10440

gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg    10500

gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg    10560

ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct    10620

catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac    10680

atttccccga aaagtgccac ctgacgt                                        10707


<210>  54
<211>  14588
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pSpCas9-PH-gagpol-D64V

<400>  54
gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc      180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt      240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata      300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      420

attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt      480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      780

gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc      840

gctagcgcta ccggtaccac catggactat aaggaccacg acggagacta caaggatcat      900

gatattgatt acaaagacga tgacgataag atggccccaa agaagaagcg gaaggtcggt      960

atccacggag tcccagcagc cgacaagaag tacagcatcg gcctggacat cggcaccaac     1020

tctgtgggct gggccgtgat caccgacgag tacaaggtgc ccagcaagaa attcaaggtg     1080

ctgggcaaca ccgaccggca cagcatcaag aagaacctga tcggagccct gctgttcgac     1140

agcggcgaaa cagccgaggc cacccggctg aagagaaccg ccagaagaag atacaccaga     1200

cggaagaacc ggatctgcta tctgcaagag atcttcagca acgagatggc caaggtggac     1260

gacagcttct tccacagact ggaagagtcc ttcctggtgg aagaggataa gaagcacgag     1320

cggcacccca tcttcggcaa catcgtggac gaggtggcct accacgagaa gtaccccacc     1380

atctaccacc tgagaaagaa actggtggac agcaccgaca aggccgacct gcggctgatc     1440

tatctggccc tggcccacat gatcaagttc cggggccact tcctgatcga gggcgacctg     1500

aaccccgaca acagcgacgt ggacaagctg ttcatccagc tggtgcagac ctacaaccag     1560

ctgttcgagg aaaaccccat caacgccagc ggcgtggacg ccaaggccat cctgtctgcc     1620

agactgagca agagcagacg gctggaaaat ctgatcgccc agctgcccgg cgagaagaag     1680

aatggcctgt tcggaaacct gattgccctg agcctgggcc tgacccccaa cttcaagagc     1740

aacttcgacc tggccgagga tgccaaactg cagctgagca aggacaccta cgacgacgac     1800

ctggacaacc tgctggccca gatcggcgac cagtacgccg acctgtttct ggccgccaag     1860

aacctgtccg acgccatcct gctgagcgac atcctgagag tgaacaccga gatcaccaag     1920

gcccccctga gcgcctctat gatcaagaga tacgacgagc accaccagga cctgaccctg     1980

ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca aagagatttt cttcgaccag     2040

agcaagaacg gctacgccgg ctacattgac ggcggagcca gccaggaaga gttctacaag     2100

ttcatcaagc ccatcctgga aaagatggac ggcaccgagg aactgctcgt gaagctgaac     2160

agagaggacc tgctgcggaa gcagcggacc ttcgacaacg gcagcatccc ccaccagatc     2220

cacctgggag agctgcacgc cattctgcgg cggcaggaag atttttaccc attcctgaag     2280

gacaaccggg aaaagatcga gaagatcctg accttccgca tcccctacta cgtgggccct     2340

ctggccaggg gaaacagcag attcgcctgg atgaccagaa agagcgagga aaccatcacc     2400

ccctggaact tcgaggaagt ggtggacaag ggcgcttccg cccagagctt catcgagcgg     2460

atgaccaact tcgataagaa cctgcccaac gagaaggtgc tgcccaagca cagcctgctg     2520

tacgagtact tcaccgtgta taacgagctg accaaagtga aatacgtgac cgagggaatg     2580

agaaagcccg ccttcctgag cggcgagcag aaaaaggcca tcgtggacct gctgttcaag     2640

accaaccgga aagtgaccgt gaagcagctg aaagaggact acttcaagaa aatcgagtgc     2700

ttcgactccg tggaaatctc cggcgtggaa gatcggttca acgcctccct gggcacatac     2760

cacgatctgc tgaaaattat caaggacaag gacttcctgg acaatgagga aaacgaggac     2820

attctggaag atatcgtgct gaccctgaca ctgtttgagg acagagagat gatcgaggaa     2880

cggctgaaaa cctatgccca cctgttcgac gacaaagtga tgaagcagct gaagcggcgg     2940

agatacaccg gctggggcag gctgagccgg aagctgatca acggcatccg ggacaagcag     3000

tccggcaaga caatcctgga tttcctgaag tccgacggct tcgccaacag aaacttcatg     3060

cagctgatcc acgacgacag cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc     3120

ggccagggcg atagcctgca cgagcacatt gccaatctgg ccggcagccc cgccattaag     3180

aagggcatcc tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat gggccggcac     3240

aagcccgaga acatcgtgat cgaaatggcc agagagaacc agaccaccca gaagggacag     3300

aagaacagcc gcgagagaat gaagcggatc gaagagggca tcaaagagct gggcagccag     3360

atcctgaaag aacaccccgt ggaaaacacc cagctgcaga acgagaagct gtacctgtac     3420

tacctgcaga atgggcggga tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc     3480

gactacgatg tggaccatat cgtgcctcag agctttctga aggacgactc catcgacaac     3540

aaggtgctga ccagaagcga caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag     3600

gtcgtgaaga agatgaagaa ctactggcgg cagctgctga acgccaagct gattacccag     3660

agaaagttcg acaatctgac caaggccgag agaggcggcc tgagcgaact ggataaggcc     3720

ggcttcatca agagacagct ggtggaaacc cggcagatca caaagcacgt ggcacagatc     3780

ctggactccc ggatgaacac taagtacgac gagaatgaca agctgatccg ggaagtgaaa     3840

gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga aggatttcca gttttacaaa     3900

gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga     3960

accgccctga tcaaaaagta ccctaagctg gaaagcgagt tcgtgtacgg cgactacaag     4020

gtgtacgacg tgcggaagat gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc     4080

aagtacttct tctacagcaa catcatgaac tttttcaaga ccgagattac cctggccaac     4140

ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg aaaccgggga gatcgtgtgg     4200

gataagggcc gggattttgc caccgtgcgg aaagtgctga gcatgcccca agtgaatatc     4260

gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag agtctatcct gcccaagagg     4320

aacagcgata agctgatcgc cagaaagaag gactgggacc ctaagaagta cggcggcttc     4380

gacagcccca ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc     4440

aagaaactga agagtgtgaa agagctgctg gggatcacca tcatggaaag aagcagcttc     4500

gagaagaatc ccatcgactt tctggaagcc aagggctaca aagaagtgaa aaaggacctg     4560

atcatcaagc tgcctaagta ctccctgttc gagctggaaa acggccggaa gagaatgctg     4620

gcctctgccg gcgaactgca gaagggaaac gaactggccc tgccctccaa atatgtgaac     4680

ttcctgtacc tggccagcca ctatgagaag ctgaagggct cccccgagga taatgagcag     4740

aaacagctgt ttgtggaaca gcacaagcac tacctggacg agatcatcga gcagatcagc     4800

gagttctcca agagagtgat cctggccgac gctaatctgg acaaagtgct gtccgcctac     4860

aacaagcacc gggataagcc catcagagag caggccgaga atatcatcca cctgtttacc     4920

ctgaccaatc tgggagcccc tgccgccttc aagtactttg acaccaccat cgaccggaag     4980

aggtacacca gcaccaaaga ggtgctggac gccaccctga tccaccagag catcaccggc     5040

ctgtacgaga cacggatcga cctgtctcag ctgggaggcg acaaaaggcc ggcggccacg     5100

aaaaaggccg gccaggcaaa aaagaaaaag aactttagcc agaactatcc gattgtgcag     5160

accggactca gatctcgagc tcaagcttcg aattctgcag tcgacggtac cgcgggcccg     5220

ggatccatgg actcgggccg ggacttcctg accctgcacg gcctacagga tgatgaggat     5280

ctacaggcgc tgctgaaggg cagccagctc ctgaaggtga agtccagctc atggaggaga     5340

gagcgcttct acaagttgca ggaggactgc aagaccatct ggcaggagtc ccgcaaggtc     5400

atgcggaccc cggagtccca gctgttctcc atcgaggaca ttcaggaggt gcgaatgggg     5460

caccgcacgg agggtctgga gaagttcgcc cgtgatgtgc ccgaggaccg ctgcttctcc     5520

attgtcttca aggaccagcg caatacacta gacctcatcg ccccatcgcc agctgatgcc     5580

cagcactggg tgctggggct gcacaagatc atccaccact caggctccat ggaccagcgt     5640

cagaagctac agcactggat tcactcctgc ttgcgaaaag ctgacaaaaa caaggacaac     5700

aagatgagct tcaaggagct gcagaacttc ctgaaggagc tcaacatcca gcctcgagcc     5760

gaattcaccc tggccgccag ggccagcgtg ctgagcggcg gcgagctgga caggtgggag     5820

aagatcaggc tgaggcccgg cggcaagaag aagtataagc tgaagcacat cgtgtgggcc     5880

agcagggagc tggagaggtt cgccgtgaac cccggcctgc tggagaccag cgagggctgc     5940

aggcagatcc tgggccagct gcagcccagc ctgcagaccg gcagcgagga gctgaggagc     6000

ctgtacaaca ccgtggccac cctgtactgc gtgcaccaga ggatcgagat caaggacacc     6060

aaggaggccc tggacaagat cgaggaggag cagaacaagt ccaagaagaa ggcccagcag     6120

gccgccgccg acaccggcca cagcagccag gtgagccaga actaccccat cgtgcagaac     6180

atccagggcc agatggtgca ccaggccatc agccccagga ccctgaacgc ctgggtgaag     6240

gtggtggagg agaaggcctt cagccccgag gtgatcccca tgttcagcgc cctgagcgag     6300

ggagccaccc cccaggacct gaacaccatg ctgaacaccg tgggcggcca ccaggccgcc     6360

atgcagatgc tgaaggagac catcaacgag gaggccgccg agtgggacag ggtgcacccc     6420

gtgcacgccg gccccatcgc ccccggccag atgagggagc cccgcggcag cgacatcgcc     6480

ggcaccacca gcaccctgca ggagcagatc ggctggatga ccaacaaccc ccccatcccc     6540

gtgggcgaaa tctacaagag gtggatcatc ctgggcctga acaagatcgt gaggatgtac     6600

agccccacca gcatcctgga tatcaggcag ggccccaaag agcccttcag ggactacgtg     6660

gacaggttct acaagaccct gcgcgccgag caggccagcc aggaggtgaa gaactggatg     6720

accgagaccc tgctggtgca gaacgccaac cccgactgca agaccatcct gaaggccctg     6780

ggacccgccg ccaccctgga ggagatgatg accgcctgcc agggcgtggg cggccccggc     6840

cacaaggcca gggtgctggc cgaggccatg agccaggtga ccaacaccgc caccatcatg     6900

atgcagaggg gcaacttcag gaaccagagg aagatggtga agtgcttcaa ctgcggcaag     6960

gagggccaca ccgccaggaa ctgccgcgcc cccaggaaga agggctgctg gaagtgcggc     7020

aaggagggcc accagatgaa ggactgcacc gagaggcagg ctaatttttt agggaagatc     7080

tggccttcct acaagggaag gccagggaat tttcttcaga gcagaccaga gccaacagcc     7140

ccaccatttc ttcagagcag accagagcca acagccccac cagaagagag cttcaggtct     7200

ggggtagaga caacaactcc ccctcagaag caggagccga tagacaagga actgtatcct     7260

ttaacttccc tcagatcact ctttggcaac gacccctcgt cacaataaag atcggtggcc     7320

agctgaagga ggccctgctg gacaccggcg ccgacgacac cgtgctggag gagatgagcc     7380

tgcccggcag gtggaagccc aagatgatcg gcggcatcgg cggcttcatc aaggtgaggc     7440

agtacgacca gatcctgatc gagatctgcg gccacaaggc catcggcacc gtgctggtgg     7500

gacctacacc tgtgaacatc atcggcagga acctgctgac ccagatcggc tgcaccctga     7560

acttccccat cagccccatc gagaccgtgc ccgtgaagct gaagcccggc atggacggcc     7620

ctaaggtgaa gcagtggccc ctgaccgagg agaagatcaa ggccctggtg gagatctgca     7680

ccgagatgga gaaggagggc aagatcagca agatcggccc cgagaacccc tacaacaccc     7740

ccgtgttcgc catcaagaag aaggacagca ccaagtggag gaagctggtg gacttcaggg     7800

agctgaacaa gaggacccag gacttctggg aggtgcagct gggcatcccc caccccgccg     7860

gcctgaagaa gaagaagagc gtgaccgtgc tggacgtggg cgacgcctac ttcagcgtgc     7920

ccctggacga ggacttcagg aagtataccg ccttcaccat ccccagcatc aacaacgaga     7980

cccccggcat ccgctaccag tacaacgtgc tgccccaggg ctggaagggc agccccgcca     8040

tcttccagag cagcatgaca aagatcctgg agcccttcaa gaagcagaac cccgacatcg     8100

tgatctatca gtacatggac gacctgtacg tgggcagcga cctggagatc ggccagcaca     8160

ggaccaagat cgaggagctg aggcagcacc tgctgaggtg gggcctgacc acccccgaca     8220

agaagcacca gaaggagccc ccattcctgt ggatgggcta cgagctgcac cccgacaagt     8280

ggaccgtgca gcccatcgtg ctgcccgaga aggacagctg gaccgtgaac gacattcaga     8340

agctggtggg caagctgaac tgggccagcc agatctaccc cggcatcaag gtgaggcagc     8400

tgtgcaagct gctgaggggc acaaaggctc tgaccgaggt gatccccctg accgaggagg     8460

ccgagctgga gctggccgag aacagggaga tcctgaagga gcccgtgcac ggcgtgtact     8520

acgaccccag caaggacctg atcgccgaga tccagaagca gggccagggc cagtggacct     8580

accagatcta ccaggagccc ttcaagaacc tgaagaccgg caagtacgcc cgcatgcgcg     8640

gcgcccacac caacgacgtg aagcagctga ccgaggccgt gcagaagatc accaccgaga     8700

gcatcgtgat ctggggcaag actcctaagt tcaagctgcc catccagaag gagacctggg     8760

agacctggtg gaccgagtac tggcaggcca cctggattcc cgagtgggag ttcgtgaaca     8820

cccctcccct ggtgaagctg tggtatcagc tggagaagga gcccatcgtg ggcgccgaga     8880

ccttctacgt ggacggcgcc gccaacaggg agaccaagct gggcaaggcc ggctacgtga     8940

ccaacaaggg ccgccagaag gtggtgcccc tgaccaacac caccaaccag aagaccgagc     9000

tgcaggctat ctacctggcc ctgcaggact caggcctgga ggtgaacatc gtgaccgaca     9060

gccagtacgc cctgggcatc atccaggccc agcccgacaa gagcgagagc gagctggtga     9120

accagatcat cgagcagctg atcaagaagg agaaggtgta cctggcctgg gtgcccgccc     9180

acaagggcat cggcggcaac gagcaggtgg acaagctggt gagcgccggc atcaggaaga     9240

tcctgttcct ggacggcatc gacaaggccc aggacgagca cgagaagtac cacagcaact     9300

ggagggctat ggctagcgac ttcaacctgc ctcccgtggt ggctaaggag atcgtggcca     9360

gctgcgacaa gtgccagctg aagggcgagg ccatgcacgg ccaggtggac tgcagccccg     9420

gcatctggca gctggtttgc acccacctgg agggcaaggt gatcctggtg gccgtgcacg     9480

tggcctccgg ctacatcgag gccgaggtga tccccgccga gaccggccag gagaccgcct     9540

acttcctgct gaagctggcc ggccgctggc ccgtgaagac catccacacc gacaacggca     9600

gcaacttcac cagcgccacc gtgaaggccg cctgctggtg ggccggcatc aagcaggagt     9660

tcggcatccc ctacaacccc cagtctcagg gcgtggtgga gagcatgaac aaggagctga     9720

agaagatcat cggccaggtg agggaccagg ccgagcacct gaagaccgcc gtgcagatgg     9780

ccgtgttcat ccacaacttc aagaggaagg gcggcatcgg cggctacagc gccggcgaga     9840

ggatcgtgga catcatcgcc accgacatcc agaccaagga gctgcagaag cagatcacca     9900

agatccagaa cttcagggtg tactacaggg acagcaggaa ccctctgtgg aagggccccg     9960

ccaagctgct gtggaagggc gagggcgccg tggtgatcca ggacaacagc gacatcaagg    10020

tggtgcccag gaggaaggcc aagatcatca gggactacgg caagcagatg gccggcgacg    10080

actgcgtggc ctccaggcag gacgaggact gactcgagca tgcatctaga gggccctatt    10140

ctatagtgtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc    10200

cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc    10260

actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct    10320

attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg    10380

catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct    10440

agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg    10500

cgcagcgtga ccgctacact tgccagcgcc ctagcgcgcg ctcctttcgc tttcttccct    10560

tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta    10620

gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt    10680

tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg    10740

ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat    10800

tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt    10860

taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt    10920

ccccaggctc cccaggcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc    10980

aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat    11040

tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt    11100

tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc    11160

gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt    11220

tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga    11280

ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg    11340

gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg    11400

ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc    11460

ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct    11520

tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa    11580

gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg    11640

gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa    11700

gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat    11760

gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg    11820

cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc    11880

atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac    11940

cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg    12000

gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc    12060

tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag    12120

cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg    12180

gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc    12240

tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca    12300

atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt    12360

ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg    12420

cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca    12480

acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca    12540

cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc    12600

attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt    12660

cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact    12720

caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag    12780

caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata    12840

ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc    12900

cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg    12960

ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc    13020

tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg    13080

gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc    13140

ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga    13200

ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg    13260

gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa    13320

aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg    13380

tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt    13440

ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat    13500

tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct    13560

aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta    13620

tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa    13680

ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac    13740

gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa    13800

gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag    13860

taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg    13920

tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag    13980

ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg    14040

tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc    14100

ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat    14160

tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata    14220

ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa    14280

aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca    14340

actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc    14400

aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc    14460

tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg    14520

aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac    14580

ctgacgtc                                                             14588


<210>  55
<211>  11232
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pZFNL(GFP)-PH-gagpol-D64V

<400>  55
gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc      180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt      240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata      300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      420

attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt      480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      780

gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc      840

gctagcgcta ccgggcgcca ccatggcccc caagaagaag aggaaggtgg gaatccatgg      900

ggtaccgggc aagaagaagc agcacatctg ccacatccag ggctgtggta aagtttacgg      960

ccgctccgac gacctgaccc gccacctgcg ctggcacacc ggcgagaggc ctttcatgtg     1020

tacatggtcc tactgtggta aacgcttcac ccagtccggc gccctggccc gccacaagcg     1080

tacccacacc ggtgagaaga aatttgcttg tccggaatgt ccgaagcgct tcatgcgctc     1140

cgaccacctg tcccgccaca tcaagaccca ccagaacaag aagggtggat cccaactagt     1200

caaaagtgaa ctggaggaga agaaatctga acttcgtcat aaattgaaat atgtgcctca     1260

tgaatatatt gaattaattg aaattgccag aaattccact caggatagaa ttcttgaaat     1320

gaaggtaatg gaatttttta tgaaagttta tggatataga ggtaaacatt tgggtggatc     1380

aaggaaaccg gacggagcaa tttatactgt cggatctcct attgattacg gtgtgatcgt     1440

ggatactaaa gcttatagcg gaggttataa tctgccaatt ggccaagcag atgaaatgca     1500

acgatatgtc gaagaaaatc aaacacgaaa caaacatatc aaccctaatg aatggtggaa     1560

agtctatcca tcttctgtaa cggaatttaa gtttttattt gtgagtggtc actttaaagg     1620

aaactacaaa gctcagctta cacgattaaa tcatatcact aattgtaatg gagctgttct     1680

tagtgtagaa gagcttttaa ttggtggaga aatgattaaa gccggcacat taaccttaga     1740

ggaagtgaga cggaaattta ataacggcga gataaacttt agccagaact atccgattgt     1800

gcagcccgga ctcagatctc gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg     1860

cccgggatcc atggactcgg gccgggactt cctgaccctg cacggcctac aggatgatga     1920

ggatctacag gcgctgctga agggcagcca gctcctgaag gtgaagtcca gctcatggag     1980

gagagagcgc ttctacaagt tgcaggagga ctgcaagacc atctggcagg agtcccgcaa     2040

ggtcatgcgg accccggagt cccagctgtt ctccatcgag gacattcagg aggtgcgaat     2100

ggggcaccgc acggagggtc tggagaagtt cgcccgtgat gtgcccgagg accgctgctt     2160

ctccattgtc ttcaaggacc agcgcaatac actagacctc atcgccccat cgccagctga     2220

tgcccagcac tgggtgctgg ggctgcacaa gatcatccac cactcaggct ccatggacca     2280

gcgtcagaag ctacagcact ggattcactc ctgcttgcga aaagctgaca aaaacaagga     2340

caacaagatg agcttcaagg agctgcagaa cttcctgaag gagctcaaca tccagcctcg     2400

agccgaattc accctggccg ccagggccag cgtgctgagc ggcggcgagc tggacaggtg     2460

ggagaagatc aggctgaggc ccggcggcaa gaagaagtat aagctgaagc acatcgtgtg     2520

ggccagcagg gagctggaga ggttcgccgt gaaccccggc ctgctggaga ccagcgaggg     2580

ctgcaggcag atcctgggcc agctgcagcc cagcctgcag accggcagcg aggagctgag     2640

gagcctgtac aacaccgtgg ccaccctgta ctgcgtgcac cagaggatcg agatcaagga     2700

caccaaggag gccctggaca agatcgagga ggagcagaac aagtccaaga agaaggccca     2760

gcaggccgcc gccgacaccg gccacagcag ccaggtgagc cagaactacc ccatcgtgca     2820

gaacatccag ggccagatgg tgcaccaggc catcagcccc aggaccctga acgcctgggt     2880

gaaggtggtg gaggagaagg ccttcagccc cgaggtgatc cccatgttca gcgccctgag     2940

cgagggagcc accccccagg acctgaacac catgctgaac accgtgggcg gccaccaggc     3000

cgccatgcag atgctgaagg agaccatcaa cgaggaggcc gccgagtggg acagggtgca     3060

ccccgtgcac gccggcccca tcgcccccgg ccagatgagg gagccccgcg gcagcgacat     3120

cgccggcacc accagcaccc tgcaggagca gatcggctgg atgaccaaca acccccccat     3180

ccccgtgggc gaaatctaca agaggtggat catcctgggc ctgaacaaga tcgtgaggat     3240

gtacagcccc accagcatcc tggatatcag gcagggcccc aaagagccct tcagggacta     3300

cgtggacagg ttctacaaga ccctgcgcgc cgagcaggcc agccaggagg tgaagaactg     3360

gatgaccgag accctgctgg tgcagaacgc caaccccgac tgcaagacca tcctgaaggc     3420

cctgggaccc gccgccaccc tggaggagat gatgaccgcc tgccagggcg tgggcggccc     3480

cggccacaag gccagggtgc tggccgaggc catgagccag gtgaccaaca ccgccaccat     3540

catgatgcag aggggcaact tcaggaacca gaggaagatg gtgaagtgct tcaactgcgg     3600

caaggagggc cacaccgcca ggaactgccg cgcccccagg aagaagggct gctggaagtg     3660

cggcaaggag ggccaccaga tgaaggactg caccgagagg caggctaatt ttttagggaa     3720

gatctggcct tcctacaagg gaaggccagg gaattttctt cagagcagac cagagccaac     3780

agccccacca tttcttcaga gcagaccaga gccaacagcc ccaccagaag agagcttcag     3840

gtctggggta gagacaacaa ctccccctca gaagcaggag ccgatagaca aggaactgta     3900

tcctttaact tccctcagat cactctttgg caacgacccc tcgtcacaat aaagatcggt     3960

ggccagctga aggaggccct gctggacacc ggcgccgacg acaccgtgct ggaggagatg     4020

agcctgcccg gcaggtggaa gcccaagatg atcggcggca tcggcggctt catcaaggtg     4080

aggcagtacg accagatcct gatcgagatc tgcggccaca aggccatcgg caccgtgctg     4140

gtgggaccta cacctgtgaa catcatcggc aggaacctgc tgacccagat cggctgcacc     4200

ctgaacttcc ccatcagccc catcgagacc gtgcccgtga agctgaagcc cggcatggac     4260

ggccctaagg tgaagcagtg gcccctgacc gaggagaaga tcaaggccct ggtggagatc     4320

tgcaccgaga tggagaagga gggcaagatc agcaagatcg gccccgagaa cccctacaac     4380

acccccgtgt tcgccatcaa gaagaaggac agcaccaagt ggaggaagct ggtggacttc     4440

agggagctga acaagaggac ccaggacttc tgggaggtgc agctgggcat cccccacccc     4500

gccggcctga agaagaagaa gagcgtgacc gtgctggacg tgggcgacgc ctacttcagc     4560

gtgcccctgg acgaggactt caggaagtat accgccttca ccatccccag catcaacaac     4620

gagacccccg gcatccgcta ccagtacaac gtgctgcccc agggctggaa gggcagcccc     4680

gccatcttcc agagcagcat gacaaagatc ctggagccct tcaagaagca gaaccccgac     4740

atcgtgatct atcagtacat ggacgacctg tacgtgggca gcgacctgga gatcggccag     4800

cacaggacca agatcgagga gctgaggcag cacctgctga ggtggggcct gaccaccccc     4860

gacaagaagc accagaagga gcccccattc ctgtggatgg gctacgagct gcaccccgac     4920

aagtggaccg tgcagcccat cgtgctgccc gagaaggaca gctggaccgt gaacgacatt     4980

cagaagctgg tgggcaagct gaactgggcc agccagatct accccggcat caaggtgagg     5040

cagctgtgca agctgctgag gggcacaaag gctctgaccg aggtgatccc cctgaccgag     5100

gaggccgagc tggagctggc cgagaacagg gagatcctga aggagcccgt gcacggcgtg     5160

tactacgacc ccagcaagga cctgatcgcc gagatccaga agcagggcca gggccagtgg     5220

acctaccaga tctaccagga gcccttcaag aacctgaaga ccggcaagta cgcccgcatg     5280

cgcggcgccc acaccaacga cgtgaagcag ctgaccgagg ccgtgcagaa gatcaccacc     5340

gagagcatcg tgatctgggg caagactcct aagttcaagc tgcccatcca gaaggagacc     5400

tgggagacct ggtggaccga gtactggcag gccacctgga ttcccgagtg ggagttcgtg     5460

aacacccctc ccctggtgaa gctgtggtat cagctggaga aggagcccat cgtgggcgcc     5520

gagaccttct acgtggacgg cgccgccaac agggagacca agctgggcaa ggccggctac     5580

gtgaccaaca agggccgcca gaaggtggtg cccctgacca acaccaccaa ccagaagacc     5640

gagctgcagg ctatctacct ggccctgcag gactcaggcc tggaggtgaa catcgtgacc     5700

gacagccagt acgccctggg catcatccag gcccagcccg acaagagcga gagcgagctg     5760

gtgaaccaga tcatcgagca gctgatcaag aaggagaagg tgtacctggc ctgggtgccc     5820

gcccacaagg gcatcggcgg caacgagcag gtggacaagc tggtgagcgc cggcatcagg     5880

aagatcctgt tcctggacgg catcgacaag gcccaggacg agcacgagaa gtaccacagc     5940

aactggaggg ctatggctag cgacttcaac ctgcctcccg tggtggctaa ggagatcgtg     6000

gccagctgcg acaagtgcca gctgaagggc gaggccatgc acggccaggt ggactgcagc     6060

cccggcatct ggcagctggt ttgcacccac ctggagggca aggtgatcct ggtggccgtg     6120

cacgtggcct ccggctacat cgaggccgag gtgatccccg ccgagaccgg ccaggagacc     6180

gcctacttcc tgctgaagct ggccggccgc tggcccgtga agaccatcca caccgacaac     6240

ggcagcaact tcaccagcgc caccgtgaag gccgcctgct ggtgggccgg catcaagcag     6300

gagttcggca tcccctacaa cccccagtct cagggcgtgg tggagagcat gaacaaggag     6360

ctgaagaaga tcatcggcca ggtgagggac caggccgagc acctgaagac cgccgtgcag     6420

atggccgtgt tcatccacaa cttcaagagg aagggcggca tcggcggcta cagcgccggc     6480

gagaggatcg tggacatcat cgccaccgac atccagacca aggagctgca gaagcagatc     6540

accaagatcc agaacttcag ggtgtactac agggacagca ggaaccctct gtggaagggc     6600

cccgccaagc tgctgtggaa gggcgagggc gccgtggtga tccaggacaa cagcgacatc     6660

aaggtggtgc ccaggaggaa ggccaagatc atcagggact acggcaagca gatggccggc     6720

gacgactgcg tggcctccag gcaggacgag gactgactcg agcatgcatc tagagggccc     6780

tattctatag tgtcacctaa atgctagagc tcgctgatca gcctcgactg tgccttctag     6840

ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac     6900

tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca     6960

ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag     7020

caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg     7080

ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt     7140

tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cgcgctcctt tcgctttctt     7200

cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc ggggcatccc     7260

tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga     7320

tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc     7380

cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt     7440

ctattctttt gatttataag ggattttggg gatttcggcc tattggttaa aaaatgagct     7500

gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga     7560

aagtccccag gctccccagg caggcagaag tatgcaaagc atgcatctca attagtcagc     7620

aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct     7680

caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc     7740

cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga     7800

ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg     7860

cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg     7920

atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg     7980

ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc     8040

cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg     8100

tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt     8160

tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg     8220

cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat     8280

catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca     8340

ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca     8400

ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa     8460

ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa     8520

tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc     8580

ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga     8640

atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc     8700

cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac     8760

caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg     8820

ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc     8880

atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa     8940

agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt     9000

ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc     9060

ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca     9120

cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa     9180

ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag     9240

ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc     9300

gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct     9360

cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg     9420

tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc     9480

cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga     9540

aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct     9600

cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg     9660

gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag     9720

ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat     9780

cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac     9840

aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac     9900

tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc     9960

ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt    10020

tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc    10080

ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg    10140

agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca    10200

atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca    10260

cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag    10320

ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac    10380

ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc    10440

agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct    10500

agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc    10560

gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg    10620

cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc    10680

gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat    10740

tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag    10800

tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat    10860

aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg    10920

cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca    10980

cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga    11040

aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc    11100

ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata    11160

tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg    11220

ccacctgacg tc                                                        11232


<210>  56
<211>  4947
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pU6-Chimeric_BB-CBh-eGFP

<400>  56
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

cgaaacaccg ggtcttcgag aagacctgtt ttagagctag aaatagcaag ttaaaataag      300

gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttg ttttagagct      360

agaaatagca agttaaaata aggctagtcc gtttttagcg cgtgcgccaa ttctgcagac      420

aaatggctct agaggtaccc gttacataac ttacggtaaa tggcccgcct ggctgaccgc      480

ccaacgaccc ccgcccattg acgtcaatag taacgccaat agggactttc cattgacgtc      540

aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc      600

caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tgtgcccagt      660

acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta      720

ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac      780

ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg      840

ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga      900

gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc      960

ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgacgc     1020

tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg     1080

accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag     1140

ctgagcaaga ggtaagggtt taagggatgg ttggttggtg gggtattaat gtttaattac     1200

ctggagcacc tgcctgaaat cacttttttt caggttggac cggtcgccac catggtgagc     1260

aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta     1320

aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg     1380

accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc     1440

accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac     1500

ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac     1560

gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc     1620

atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag     1680

tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag     1740

gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac     1800

cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc     1860

acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag     1920

ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagcc tagagctcgc     1980

tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg     2040

ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt     2100

gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc     2160

aagggggagg attgggaaga gaatagcagg catgctgggg agcggccgca ggaaccccta     2220

gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca     2280

aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc     2340

tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac     2400

cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg     2460

tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg     2520

ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg     2580

ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt     2640

tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt     2700

tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta     2760

tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa     2820

atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt     2880

tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc     2940

cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac     3000

aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac     3060

gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa     3120

tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt     3180

tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc     3240

ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc     3300

ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa     3360

aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg     3420

gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag     3480

ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc     3540

gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta     3600

cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg     3660

cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca     3720

acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac     3780

caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat     3840

taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg     3900

ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata     3960

aatctggagc cggtgagcgt ggaagccgcg gtatcattgc agcactgggg ccagatggta     4020

agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa     4080

atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag     4140

tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg     4200

tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact     4260

gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg     4320

taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc     4380

aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata     4440

ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta     4500

catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc     4560

ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg     4620

ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac     4680

agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg     4740

taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt     4800

atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct     4860

cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg     4920

ccttttgctg gccttttgct cacatgt                                         4947


<210>  57
<211>  4957
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pU6-SpCas9_sgRNA2.0-CBh-eGFP

<400>  57
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

cgaaacaccg ggtcttcgag aagacctgtt taagagctat gctggaaaca gcatagcaag      300

tttaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttg      360

ttttagagct agaaatagca agttaaaata aggctagtcc gtttttagcg cgtgcgccaa      420

ttctgcagac aaatggctct agaggtaccc gttacataac ttacggtaaa tggcccgcct      480

ggctgaccgc ccaacgaccc ccgcccattg acgtcaatag taacgccaat agggactttc      540

cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg      600

tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat      660

tgtgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc      720

atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc      780

ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg      840

gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg      900

gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt      960

atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc     1020

gctgcgacgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg     1080

gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg     1140

ctgtaattag ctgagcaaga ggtaagggtt taagggatgg ttggttggtg gggtattaat     1200

gtttaattac ctggagcacc tgcctgaaat cacttttttt caggttggac cggtcgccac     1260

catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga     1320

cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta     1380

cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac     1440

cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa     1500

gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt     1560

cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct     1620

ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca     1680

caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa     1740

cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc     1800

cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca     1860

ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt     1920

cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagcc     1980

tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc     2040

ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa     2100

tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg     2160

gcaggacagc aagggggagg attgggaaga gaatagcagg catgctgggg agcggccgca     2220

ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc     2280

cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg     2340

agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg     2400

tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc     2460

gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc     2520

gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct     2580

ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa     2640

aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc     2700

cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca     2760

ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat     2820

tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg     2880

tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag     2940

ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc     3000

gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca     3060

tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc     3120

atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc     3180

cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc     3240

tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc     3300

gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg     3360

gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat     3420

ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc     3480

acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa     3540

ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa     3600

aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt     3660

gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct     3720

tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat     3780

gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg     3840

cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg     3900

atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt     3960

attgctgata aatctggagc cggtgagcgt ggaagccgcg gtatcattgc agcactgggg     4020

ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg     4080

gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg     4140

tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa     4200

aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt     4260

tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt     4320

tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt     4380

ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag     4440

ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta     4500

gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat     4560

aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg     4620

ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg     4680

agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac     4740

aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga     4800

aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt     4860

ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta     4920

cggttcctgg ccttttgctg gccttttgct cacatgt                              4957


<210>  58
<211>  4967
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pU6-SpCas9_sgRNA2.1-CBh-eGFP

<400>  58
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

cgaaacaccg ggtcttcgag aagacctgtt taagagctat gctggaaaca gcatagcaag      300

tttaaataag gctagtccgt tatcaacttt gctggaaaca gcaaagtggc accgagtcgg      360

tgcttttttg ttttagagct agaaatagca agttaaaata aggctagtcc gtttttagcg      420

cgtgcgccaa ttctgcagac aaatggctct agaggtaccc gttacataac ttacggtaaa      480

tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaatag taacgccaat      540

agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt      600

acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc      660

cgcctggcat tgtgcccagt acatgacctt atgggacttt cctacttggc agtacatcta      720

cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc      780

catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc      840

agcgatgggg gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg      900

gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa      960

gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg     1020

ggcgggagtc gctgcgacgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc     1080

gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt     1140

ctcctccggg ctgtaattag ctgagcaaga ggtaagggtt taagggatgg ttggttggtg     1200

gggtattaat gtttaattac ctggagcacc tgcctgaaat cacttttttt caggttggac     1260

cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg     1320

tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg     1380

atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc     1440

cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg     1500

accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc     1560

gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg     1620

gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca     1680

tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca     1740

agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg     1800

tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc     1860

ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg     1920

atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc     1980

tgtacaagcc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg     2040

ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt     2100

cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg     2160

gtggggtggg gcaggacagc aagggggagg attgggaaga gaatagcagg catgctgggg     2220

agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc     2280

tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag     2340

tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc     2400

atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg     2460

cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc     2520

cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc     2580

ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct     2640

cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac     2700

ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac     2760

tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat     2820

ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa     2880

aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata     2940

gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct     3000

cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt     3060

ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata     3120

ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt     3180

gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag     3240

acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca     3300

tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc     3360

agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat     3420

cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc     3480

aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg     3540

gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc     3600

agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat     3660

aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga     3720

gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc     3780

ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc     3840

aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt     3900

aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc     3960

tggctggttt attgctgata aatctggagc cggtgagcgt ggaagccgcg gtatcattgc     4020

agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca     4080

ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca     4140

ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt     4200

ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta     4260

acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg     4320

agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc     4380

ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag     4440

cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa     4500

gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc     4560

cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc     4620

gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta     4680

caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag     4740

aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct     4800

tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga     4860

gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc     4920

ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt                   4967


<210>  59
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer

<400>  59
ccttcagctc agtgacagtg g                                                 21


<210>  60
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer

<400>  60
ccgactcggt gccacttt                                                     18


<210>  61
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  61
aaataaggct agtccgttat caactt                                            26


<210>  62
<211>  5
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Extension A

<400>  62
ugcug                                                                    5


<210>  63
<211>  5
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Extension B

<400>  63
cagca                                                                    5


<210>  64
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SERPING1 - Fw primer

<400>  64
ctcatctgcc gcactgtcag aaattactct                                        30


<210>  65
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SERPING1 - Rv primer

<400>  65
gattggtgac tcttatggga gtgtccaaca                                        30


<210>  66
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  P1

<400>  66
ccuucagcuc agugacagug g                                                 21


<210>  67
<211>  26
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  P2

<400>  67
aaauaaggcu aguccguuau caacuu                                            26


<210>  68
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  P3

<400>  68
aaaguggcac cgagucgg                                                     18


<210>  69
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ddPCR_Pcsk9 Fwd primer

<400>  69
cagcagcggc ggcaacaggt                                                   20


<210>  70
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ddPCR_VEGFA Fwd primer

<400>  70
cctggaagat gtccaccagt                                                   20


<210>  71
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ddPCR Serping1 Fwd primer

<400>  71
ttgcaagaca gaggcgaagt                                                   20


<210>  72
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ChIP_Pcsk9 Fwd primer

<400>  72
ggctcccgtt ctctctctct                                                   20


<210>  73
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ChIP_Pcsk9 Rev primer

<400>  73
ctcgggaagg acatggacg                                                    19


<210>  74
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ChIP Reference Fwd primer

<400>  74
ctgctcctga ctgggtaatg aa                                                22


<210>  75
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ChIP Reference Rev primer

<400>  75
attttgtgct gcataacctc ct                                                22


<210>  76
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Seq_Vegfa_Nested1_Fwd primer

<400>  76
gagcccaggg agcaaaggtc a                                                 21


<210>  77
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Seq_Vegfa_Nested1_Rev primer

<400>  77
ggttcctggt tgtgcttagt ggga                                              24


<210>  78
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Seq_Vegfa_Nested2-P1_Fwd primer

<400>  78
aaaggtcacg aaagcagatg gtcaa                                             25


<210>  79
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Seq_Vegfa_Nested2-P1_Rev primer

<400>  79
gtgtatatac atagctgtcc ccgg                                              24


<210>  80
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Seq_Vegfa_Nested2-P2_Fwd primer

<400>  80
agagcttcgg cagggaagta ca                                                22


<210>  81
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Seq_Vegfa_Nested2-P2_Rev primer

<400>  81
tatttgatga gtggctgttg gcct                                              24


<210>  82
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Seq_Vegfa_Nested2-P3_Fwd primer

<400>  82
ttgtaccact gtcctcctgc                                                   20


<210>  83
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Seq_Vegfa_Nested2-P3_Rev primer

<400>  83
gtggagctgt aaggagtggt                                                   20


<210>  84
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SA1

<400>  84
tgtcgacgat atcttcgaac atggtgagca agggcgag                               38


<210>  85
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SA2

<400>  85
acggccgcta tgctttactg ctacacattg atcctagcag aag                         43


<210>  86
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  JH9

<400>  86
ctcttcctca tctccgggcc tttcgacctc tagcgaatgt cctttattcc agtggccg         58


<210>  87
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  JH10

<400>  87
gctgggccac caggcattac tgctccagtc gttcatgagg ac                          42


<210>  88
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  JH11

<400>  88
cctcatgaac gactggagca gtaatgcctg gtggcccag                              39


<210>  89
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  JH12

<400>  89
ccattgctgg atgtctcgtg ctatgaaaag acagcaagct taggtct                     47


<210>  90
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  JH13

<400>  90
agcttgctgt cttttcatag cacgagacat ccagcaatgg g                           41


<210>  91
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  JH14

<400>  91
gagaagtttg ttgcgccgga tccggctggt gaaagggcag gcag                        44


<210>  92
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  JH15

<400>  92
tgcctgccct ttcaccagcc ggatccggcg caacaaact                              39


<210>  93
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  JH16

<400>  93
gcggaattta cggccgctat gctttactgc cttgtacagc tcgtccatg                   49


<210>  94
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  D2eGFP - sequence

<400>  94
cggtgaacag ctcctcgccc                                                   20


<210>  95
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCSK9 off target - Fw primer

<400>  95
gtacaaggct ccacaggtca                                                   20


<210>  96
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCSK9 off target - Rv primer

<400>  96
ctcctgctct aggagaggtg t                                                 21


<210>  97
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VEGFA site 1 off target1 - Fw primer

<400>  97
ggagactgtt tggcctctgt                                                   20


<210>  98
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VEGFA  site 1 off target1 - Rv primer

<400>  98
aatagtcccc tgttcgtgcc                                                   20


<210>  99
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VEGFA  site 1 off target2 - Fw primer

<400>  99
gctggtccct gtggattaca                                                   20


<210>  100
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VEGFA  site 1 off target2 - Rv primer

<400>  100
tgagagactt cggggacaga                                                   20


<210>  101
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fah - sequence

<400>  101
ggatggtcct catgaacgac                                                   20


<210>  102
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fah - Fw primer

<400>  102
gttgccaaat tggctccact tac                                               23


<210>  103
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fah - Rv primer

<400>  103
ccacacccac agagtcagaa                                                   20


<210>  104
<211>  116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA2.1 d2eGFP

<400>  104
gggcgaggag cuguucaccg guuuaagagc uaugcuggaa acagcauagc aaguuuaaau       60

aaggcuaguc cguuaucaac uuugcuggaa acagcaaagu ggcaccgagu cggugc          116


<210>  105
<211>  116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA2.1 Fah

<400>  105
ggaugguccu caugaacgac guuuaagagc uaugcuggaa acagcauagc aaguuuaaau       60

aaggcuaguc cguuaucaac uuugcuggaa acagcaaagu ggcaccgagu cggugc          116


<210>  106
<211>  8251
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCCL/PGK-MCS-IRES-puro

<400>  106
aagcttggcc attgcatacg ttgtatccat atcataatat gtacatttat attggctcat       60

gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag taatcaatta      120

cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg      180

gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc      240

ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa      300

ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca      360

atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta      420

cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt      480

acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg      540

acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca      600

actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca      660

gagctcgttt agtgaaccgg ggtctctctg gttagaccag atctgagcct gggagctctc      720

tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt      780

agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc      840

agtgtggaaa atctctagca gtggcgcccg aacagggacc tgaaagcgaa agggaaacca      900

gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg      960

cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc     1020

gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg     1080

ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa     1140

cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga     1200

cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta     1260

gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac     1320

aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt     1380

cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt     1440

agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag     1500

agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag     1560

cactatgggc gcagcctcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat     1620

agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact     1680

cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa     1740

ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt     1800

gccttggaat gctagttgga gtaataaatc tctggaacag attggaatca cacgacctgg     1860

atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa     1920

tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt     1980

ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata     2040

gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt     2100

aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac     2160

aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta     2220

gtgaacggat ctcgacggta tcggttaact tttaaaagaa aaggggggat tggggggtac     2280

agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa     2340

aaacaaatta caaaaattca aaattttatc gatcacgaga ctagcctcga cgatggtcga     2400

gtaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc     2460

gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg     2520

taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct     2580

agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt     2640

agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg     2700

cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg     2760

aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag     2820

gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc     2880

ctcttcctca tctccgggcc tttcgacctc tagcgacgcg tgtcgacgat atcttcgaag     2940

gatccagtaa agcatagcgg ccgtaaattc cgcccctctc tccctccccc ccccctaacg     3000

ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca     3060

ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga     3120

gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga     3180

aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca     3240

ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag     3300

atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa     3360

gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac     3420

cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga     3480

ggttaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg     3540

atgataagct tgccacaacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg     3600

acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc     3660

gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc     3720

tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg     3780

cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc     3840

gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg     3900

cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc     3960

accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg     4020

ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc     4080

tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga     4140

cccgcaagcc cggtgcctga ctcgagggaa ttccgataat caacctctgg attacaaaat     4200

ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc     4260

tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt     4320

gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg     4380

cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg     4440

tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc     4500

cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt     4560

gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct     4620

gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg     4680

cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg     4740

gatctccctt tgggccgcct ccccgcatcg ggaattcgag ctcggtacct ttaagaccaa     4800

tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag     4860

ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt     4920

tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc     4980

aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta     5040

actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagctag     5100

aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta     5160

tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt gtagcatctg     5220

gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc ggtttggttg     5280

gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa     5340

tcagcagggt catcgctagc ctaggctttt gcgtcgagac gtacccaatt cgccctatag     5400

tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc     5460

tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag     5520

cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg     5580

cgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac     5640

cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc     5700

cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt     5760

tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg     5820

gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag     5880

tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt     5940

ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt     6000

taacgcgaat tttaacaaaa tattaacgtt tacaatttcc caggtggcac ttttcgggga     6060

aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc     6120

atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt     6180

caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct     6240

cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt     6300

tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt     6360

tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac     6420

gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac     6480

tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct     6540

gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg     6600

aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg     6660

gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca     6720

atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa     6780

caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt     6840

ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc     6900

attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg     6960

agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt     7020

aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt     7080

catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc     7140

ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct     7200

tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta     7260

ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc     7320

ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac     7380

ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct     7440

gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat     7500

aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg     7560

acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa     7620

gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg     7680

gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga     7740

cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc     7800

aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct     7860

gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct     7920

cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca     7980

atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg     8040

tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat     8100

taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc     8160

ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac     8220

cctcactaaa gggaacaaaa gctggagctg c                                    8251


<210>  107
<211>  6475
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pT2/UASTK-d2eGFP-SV40-neo

<400>  107
caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg       60

ccagtgagcg cgcgtaatac gactcactat agggcgaatt gcaattggaa ttcggatccc      120

tatacagttg aagtcggaag tttacataca cttaagttgg agtcattaaa actcgttttt      180

caactactcc acaaatttct tgttaacaaa caatagtttt ggcaagtcag ttaggacatc      240

tactttgtgc atgacacaag tcatttttcc aacaattgtt tacagacaga ttatttcact      300

tataattcac tgtatcacaa ttccagtggg tcagaagttt acatacacta agttgactgt      360

gcctttaaac agcttggaaa attccagaaa atgatgtcat ggctttaggt ttaaaccaag      420

gcggagtact gtcctccggg ctggcggagt actgtcctcc ggcaaggtcg gagtactgtc      480

ctccgacact agaggtcgga gtactgtcct ccgacggtgg ccgccccgac tgcatctgcg      540

tgttcaaatt cgccaatgac aagacgctgg gcggggtttg tgtcatcata gaactaaaga      600

catgcaaata tatttcttcc ggggacaccg ccagcaaacg cgagcaacgg gccacgggga      660

tgaagcagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg      720

agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg      780

ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct      840

ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg cttcagccgc taccccgacc      900

acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca      960

ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg     1020

acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc     1080

tggggcacaa gctggagtac aactacatca gccacaacgt ctatatcacc gccgacaagc     1140

agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcagcgtgc     1200

agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg     1260

acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc     1320

acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt     1380

acaagaagct tagccatggc ttcccgccgg aggtggagga gcaggatgat ggcacgctgc     1440

ccatgtcttg tgcccaggag agcgggatgg accgtcaccc tgcagcctgt gcttctgcta     1500

ggatcaatgt gtagatgcgc ggccgcgact ctagatcata atcagccata ccacatttgt     1560

agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat     1620

gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa     1680

tagcatcaca aatttcacaa ataaagcatt tgtttaaacc ccacaataag ttgggtgaat     1740

tttggctcat tcctcctttc tataggattg aggtcagagc tttgtgatgg gaattctgtg     1800

gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccaggcaggc agaagtatgc     1860

aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag     1920

gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc     1980

cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa     2040

ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt     2100

gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tggatatcca     2160

ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat     2220

tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac     2280

agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc     2340

tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc     2400

tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag     2460

cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc     2520

ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg     2580

atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc     2640

ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc     2700

cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga     2760

cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca     2820

tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg     2880

atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg     2940

ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg     3000

gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga     3060

ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg     3120

gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat     3180

tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt     3240

tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg     3300

gatcccttga aatacatcca caggtacacc tccaattgac tcaaatgatg tcaattagtc     3360

tatcaggagc tctttaagct tgtggaaggc tactcgaaat gtttgaccca agttaaacaa     3420

tttaaaggca atgctaccaa atactaattg agtgtatgta aacttctgac ccactgggaa     3480

tgtgatgaaa gaaataaaag ctgaaatgaa tcattctctc tactattatt ctgatatttc     3540

acattcttaa aataaagtgg tgatcctaac tgacctaaga cagggaattt ttactaggat     3600

taaatgtcag gaattgtgaa aaagtgagtt taaatgtatt tggctaaggt gtatgtaaac     3660

ttccgacttc aactgtatag ggatcctcta gctagagtcg acctcgaggg ggggcccggt     3720

acccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata     3780

gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag     3840

cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg     3900

ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca     3960

acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc     4020

gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg     4080

gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa     4140

ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga     4200

cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag     4260

ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct     4320

taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg     4380

ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc     4440

ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt     4500

aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta     4560

tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac     4620

agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc     4680

ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat     4740

tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc     4800

tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt     4860

cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta     4920

aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct     4980

atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg     5040

cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga     5100

tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt     5160

atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt     5220

taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt     5280

tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat     5340

gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc     5400

cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc     5460

cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat     5520

gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag     5580

aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt     5640

accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc     5700

ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa     5760

gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg     5820

aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa     5880

taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag     5940

cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag     6000

cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt     6060

tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca     6120

cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata     6180

gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca     6240

aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc     6300

gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa     6360

caaaatatta acgcttacaa tttccattcg ccattcaggc tgcgcaactg ttgggaaggg     6420

cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctg          6475


<210>  108
<211>  7726
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCCL-PGK-mCherry

<400>  108
aagcttggcc attgcatacg ttgtatccat atcataatat gtacatttat attggctcat       60

gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag taatcaatta      120

cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg      180

gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc      240

ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa      300

ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca      360

atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta      420

cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt      480

acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg      540

acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca      600

actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca      660

gagctcgttt agtgaaccgg ggtctctctg gttagaccag atctgagcct gggagctctc      720

tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt      780

agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc      840

agtgtggaaa atctctagca gtggcgcccg aacagggacc tgaaagcgaa agggaaacca      900

gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg      960

cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc     1020

gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg     1080

ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa     1140

cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga     1200

cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta     1260

gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac     1320

aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt     1380

cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt     1440

agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag     1500

agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag     1560

cactatgggc gcagcctcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat     1620

agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact     1680

cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa     1740

ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt     1800

gccttggaat gctagttgga gtaataaatc tctggaacag attggaatca cacgacctgg     1860

atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa     1920

tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt     1980

ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata     2040

gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt     2100

aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac     2160

aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta     2220

gtgaacggat ctcgacggta tcggttaact tttaaaagaa aaggggggat tggggggtac     2280

agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa     2340

aaacaaatta caaaaattca aaattttatc gatcacgaga ctagcctcga cgatggtcga     2400

gtaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc     2460

gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg     2520

taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct     2580

agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt     2640

agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg     2700

cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg     2760

aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag     2820

gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc     2880

ctcttcctca tctccgggcc tttcgacctc tagcgggatc catggtgagc aagggcgagg     2940

aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg     3000

tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc     3060

agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt     3120

cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact     3180

acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg     3240

gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg     3300

tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg     3360

gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca     3420

agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca     3480

aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca     3540

cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact     3600

ccaccggcgg catggacgag ctgtacaaga attgactcga gggaattccg ataatcaacc     3660

tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac     3720

gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt     3780

cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt     3840

tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg     3900

cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac     3960

ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac     4020

tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt     4080

tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc     4140

ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg     4200

ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgggaat tcgagctcgg     4260

tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa     4320

aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta     4380

ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc     4440

cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt     4500

tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta     4560

gcagcatcta gctagaatta attccgtgta ttctatagtg tcacctaaat cgtatgtgta     4620

tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg tacaagccta     4680

attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa actgccgtca     4740

gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg cacccggaaa     4800

tggtcagcga accaatcagc agggtcatcg ctagcctagg cttttgcgtc gagacgtacc     4860

caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg     4920

tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc     4980

cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct     5040

gaatggcgaa tggcgcgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt     5100

tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt     5160

cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc     5220

tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga     5280

tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc     5340

cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt     5400

ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct     5460

gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa tttcccaggt     5520

ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca     5580

aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg     5640

aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc     5700

cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg     5760

ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt     5820

cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta     5880

ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat     5940

gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga     6000

gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca     6060

acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact     6120

cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc     6180

acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact     6240

ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt     6300

ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt     6360

gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt     6420

atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata     6480

ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag     6540

attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat     6600

ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa     6660

aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca     6720

aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt     6780

ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg     6840

tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc     6900

ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga     6960

cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc     7020

agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc     7080

gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca     7140

ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg     7200

tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta     7260

tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct     7320

cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag     7380

tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa     7440

gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc     7500

agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg     7560

agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg     7620

tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc     7680

aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgc                    7726


<210>  109
<211>  10881
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCCL/PGK-FahMut-P2A-eGFP-IRES-puro

<400>  109
aagcttggcc attgcatacg ttgtatccat atcataatat gtacatttat attggctcat       60

gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag taatcaatta      120

cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg      180

gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc      240

ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa      300

ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca      360

atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta      420

cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt      480

acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg      540

acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca      600

actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca      660

gagctcgttt agtgaaccgg ggtctctctg gttagaccag atctgagcct gggagctctc      720

tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt      780

agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc      840

agtgtggaaa atctctagca gtggcgcccg aacagggacc tgaaagcgaa agggaaacca      900

gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg      960

cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc     1020

gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg     1080

ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa     1140

cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga     1200

cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta     1260

gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac     1320

aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt     1380

cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt     1440

agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag     1500

agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag     1560

cactatgggc gcagcctcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat     1620

agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact     1680

cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa     1740

ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt     1800

gccttggaat gctagttgga gtaataaatc tctggaacag attggaatca cacgacctgg     1860

atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa     1920

tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt     1980

ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata     2040

gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt     2100

aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac     2160

aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta     2220

gtgaacggat ctcgacggta tcggttaact tttaaaagaa aaggggggat tggggggtac     2280

agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa     2340

aaacaaatta caaaaattca aaattttatc gatcacgaga ctagcctcga cgatggtcga     2400

gtaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc     2460

gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg     2520

taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct     2580

agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt     2640

agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg     2700

cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg     2760

aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag     2820

gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc     2880

ctcttcctca tctccgggcc tttcgacctc tagcgaatgt cctttattcc agtggccgag     2940

gactccgact ttcccatcca aaacctgccc tatggtgttt tctccactca aagcaaccca     3000

aagccacgga ttggtgtagc catcggtgac cagatcttgg acctgagtgt cattaaacac     3060

ctctttaccg gacctgccct ttccaaacat caacatgtct tcgatgagac aactctcaat     3120

aacttcatgg gtctgggtca agctgcatgg aaggaggcaa gagcatcctt acagaactta     3180

ctgtctgcca gccaagcccg gctcagagat gacaaggagc ttcggcagcg tgcattcacc     3240

tcccaggctt ctgcgacaat gcaccttcct gctaccatag gagactacac ggacttctac     3300

tcttctcggc agcatgccac caatgttggc attatgttca gaggcaagga gaatgcgctg     3360

ttgccaaatt ggctccactt acctgtggga taccatggcc gagcttcctc cattgtggta     3420

tctggaaccc cgattcgaag acccatgggg cagatgagac ctgataactc aaagcctcct     3480

gtgtatggtg cctgcagact cttagacatg gagttggaaa tggctttctt cgtaggccct     3540

gggaacagat tcggagagcc aatccccatt tccaaagccc atgaacacat tttcgggatg     3600

gtcctcatga acgactggag cagtaatgcc tggtggccca gcttcctctg atgttctgtt     3660

cttaggggca cacacaggag ttgggtatgg gacaggaggc ctaagtacta caggggtgat     3720

accatgcaga cttctgactc tgtgggtgtg gggcagtcac agcttccctg agtagctttc     3780

tcataagtgg aaggatggag ctgacagaac ctaaagcttt atcaagccct acacactcca     3840

ctcactgttg ccagcaccat cagctgctga tgaagagctg tacatggagg gattttagtc     3900

agtggctgaa ttgagcctta ctattcatca gcccagcatc tgagtcccag ctctacaccc     3960

tgataccatg tgtaagagga agtgggtcca gaaatctcag gagagggcct tcccccattc     4020

aggcccttgg tagggactgt cctgtcatcc ttcattgttc cctagcacag cacctccaag     4080

gacctctgtc ttcatactga atacctttct gtttgttccc tgggagatct ggttggccag     4140

agccatgtag agaggtccat gtaggtcaag gacaggaagc agcttggagt gagtctgtaa     4200

gccacggtga gacctaagct tgctgtcttt tcatagcacg agacatccag caatgggagt     4260

acgtcccact tgggccattc ctggggaaaa gctttggaac cacaatctcc ccgtgggtgg     4320

tgcctatgga tgccctcatg ccctttgtgg tgccaaaccc aaagcaggac cccaagccct     4380

tgccatatct ctgccacagc cagccctaca catttgatat caacctgtct gtctctttga     4440

aaggagaagg aatgagccag gcggctacca tctgcaggtc taactttaag cacatgtact     4500

ggaccatgct gcagcaactc acacaccact ctgttaatgg atgcaacctg agacctgggg     4560

acctcttggc ttctggaacc atcagtggat cagaccctga aagctttggc tccatgctgg     4620

aactgtcctg gaagggaaca aaggccatcg atgtggagca ggggcagacc aggaccttcc     4680

tgctggacgg cgatgaagtc atcataacag gtcactgcca gggggacggc taccgtgttg     4740

gctttggcca gtgtgctggg aaagtgctgc ctgccctttc accagccgga tccggcgcaa     4800

caaacttctc tctgctgaaa caagccggag atgtcgaaga gaatcctgga ccggtgagca     4860

agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa     4920

acggccacaa gttcagcgtg tctggcgagg gcgagggcga tgccacctac ggcaagctga     4980

ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca     5040

ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact     5100

tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg     5160

acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca     5220

tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt     5280

acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg     5340

cgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc     5400

agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca     5460

cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt     5520

tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggca gtaaagcata     5580

gcggccgtaa attccgcccc cccccccctc tccctccccc ccccctaacg ttactggccg     5640

aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc     5700

gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag     5760

gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt     5820

tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa     5880

ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc     5940

aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg     6000

gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat     6060

gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa     6120

cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagct     6180

tgccacaacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc     6240

ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt     6300

cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt     6360

cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac     6420

cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga     6480

gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg     6540

gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa     6600

gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc     6660

cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac     6720

cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc     6780

cggtgcctga ctcgagggaa ttccgataat caacctctgg attacaaaat ttgtgaaaga     6840

ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg     6900

cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc     6960

tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc     7020

actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt     7080

tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt     7140

gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg     7200

aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg     7260

tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg     7320

ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt     7380

tgggccgcct ccccgcatcg ggaattcgag ctcggtacct ttaagaccaa tgacttacaa     7440

ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca     7500

ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat     7560

ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt     7620

gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc     7680

cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagctag aattaattcc     7740

gtgtattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta tgtattaatt     7800

gtagccgcgt tctaacgaca atatgtacaa gcctaattgt gtagcatctg gcttactgaa     7860

gcagacccta tcatctctct cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt     7920

ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt     7980

catcgctagc ctaggctttt gcgtcgagac gtacccaatt cgccctatag tgagtcgtat     8040

tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc     8100

caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc     8160

cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cgacgcgccc     8220

tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt     8280

gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc     8340

ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta     8400

cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc     8460

tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg     8520

ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt     8580

ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat     8640

tttaacaaaa tattaacgtt tacaatttcc caggtggcac ttttcgggga aatgtgcgcg     8700

gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat     8760

aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc     8820

gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa     8880

cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac     8940

tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga     9000

tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag     9060

agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca     9120

cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca     9180

tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa     9240

ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc     9300

tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa     9360

cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag     9420

actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct     9480

ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac     9540

tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa     9600

ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt     9660

aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat     9720

ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg     9780

agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc     9840

ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg     9900

tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag     9960

cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact    10020

ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg    10080

gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc    10140

ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg    10200

aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg    10260

cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag    10320

ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc    10380

gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct    10440

ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc    10500

ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc    10560

gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac    10620

cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact    10680

ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc    10740

aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat    10800

ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa    10860

gggaacaaaa gctggagctg c                                              10881


<210>  110
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VEGFA site 1 - sequence

<400>  110
ctcctggaag atgtccacca                                                   20


<210>  111
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VEGFA site 1 - Fw primer

<400>  111
gagcccaggg agcaaaggtc a                                                 21


<210>  112
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VEGFA site 1 - Rv primer

<400>  112
ggttcctggt tgtgcttagt ggga                                              24


<210>  113
<211>  116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA2.1 VEGFA(site 1)

<400>  113
cuccuggaag auguccacca guuuaagagc uaugcuggaa acagcauagc aaguuuaaau       60

aaggcuaguc cguuaucaac uuugcuggaa acagcaaagu ggcaccgagu cggugc          116


