﻿               SEQUENCE LISTING

<110> Max-Delbrück-Centrum für Molekulare Medizin Berlin-Buch  

<120> TCR constructs specific for MAGEA4-derived epitopes

<130> 13345 P 6581 WO

<160> 27

<170> BiSSAP 1.3.6

<210> 1
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> MAGEA4 epitope presented on HLA-A*01:01

<400> 1
Glu Val Asp Pro Ala Ser Asn Thr Tyr 
1               5                   

<210> 2
<211> 5
<212> PRT
<213> Homo sapiens


<220> 
<223> TRA CDR1, aa

<400> 2
Ser Ile Phe Asn Thr 
1               5   

<210> 3
<211> 7
<212> PRT
<213> Homo sapiens


<220> 
<223> TRA CDR2, aa

<400> 3
Leu Tyr Lys Ala Gly Glu Leu 
1               5           

<210> 4
<211> 13
<212> PRT
<213> Homo sapiens


<220> 
<223> TRA CDR3, aa

<400> 4
Ala Gly Gln Leu Trp Gly Gly Ser Asn Tyr Lys Leu Thr 
1               5                   10              

<210> 5
<211> 5
<212> PRT
<213> Homo sapiens


<220> 
<223> TRB CDR1, aa

<400> 5
Ser Gly His Arg Ser 
1               5   

<210> 6
<211> 6
<212> PRT
<213> Homo sapiens


<220> 
<223> TRB CDR2, aa

<400> 6
Tyr Phe Ser Glu Thr Gln 
1               5       

<210> 7
<211> 18
<212> PRT
<213> Homo sapiens


<220> 
<223> TRB CDR3, aa

<400> 7
Ala Ser Ser Leu Ala Gly Thr Gly Ala His Phe Ile Thr Asn Glu Lys 
1               5                   10                  15      
Leu Phe 
        

<210> 8
<211> 92
<212> PRT
<213> Homo sapiens


<220> 
<223> TRA variable region, aa

<400> 8
Gly Gln Gln Leu Asn Gln Ser Pro Gln Ser Met Phe Ile Gln Glu Gly 
1               5                   10                  15      
Glu Asp Val Ser Met Asn Cys Thr Ser Ser Ser Ile Phe Asn Thr Trp 
            20                  25                  30          
Leu Trp Tyr Lys Gln Glu Pro Gly Glu Gly Pro Val Leu Leu Ile Ala 
        35                  40                  45              
Leu Tyr Lys Ala Gly Glu Leu Thr Ser Asn Gly Arg Leu Thr Ala Gln 
    50                  55                  60                  
Phe Gly Ile Thr Arg Lys Asp Ser Phe Leu Asn Ile Ser Ala Ser Ile 
65                  70                  75                  80  
Pro Ser Asp Val Gly Ile Tyr Phe Cys Ala Gly Gln 
                85                  90          

<210> 9
<211> 95
<212> PRT
<213> Homo sapiens


<220> 
<223> TRB variable region, aa

<400> 9
Lys Ala Gly Val Thr Gln Thr Pro Arg Tyr Leu Ile Lys Thr Arg Gly 
1               5                   10                  15      
Gln Gln Val Thr Leu Ser Cys Ser Pro Ile Ser Gly His Arg Ser Val 
            20                  25                  30          
Ser Trp Tyr Gln Gln Thr Pro Gly Gln Gly Leu Gln Phe Leu Phe Glu 
        35                  40                  45              
Tyr Phe Ser Glu Thr Gln Arg Asn Lys Gly Asn Phe Pro Gly Arg Phe 
    50                  55                  60                  
Ser Gly Arg Gln Phe Ser Asn Ser Arg Ser Glu Met Asn Val Ser Thr 
65                  70                  75                  80  
Leu Glu Leu Gly Asp Ser Ala Leu Tyr Leu Cys Ala Ser Ser Leu 
                85                  90                  95  

<210> 10
<211> 15
<212> PRT
<213> Homo sapiens


<220> 
<223> TRA junction aa

<400> 10
Cys Ala Gly Gln Leu Trp Gly Gly Ser Asn Tyr Lys Leu Thr Phe 
1               5                   10                  15  

<210> 11
<211> 20
<212> PRT
<213> Homo sapiens


<220> 
<223> TRB junction aa

<400> 11
Cys Ala Ser Ser Leu Ala Gly Thr Gly Ala His Phe Ile Thr Asn Glu 
1               5                   10                  15      
Lys Leu Phe Phe 
            20  

<210> 12
<211> 276
<212> DNA
<213> Homo sapiens


<220> 
<223> TRA variable region, codon-optimized na

<400> 12
ggccagcagc tgaatcagag cccacagagc atgttcatcc aagaaggcga ggacgtttcc     60

atgaactgca ccagcagcag catcttcaac acctggctgt ggtacaagca agagcctggc    120

gaaggacccg tgctgctgat tgccttgtat aaggccggcg agctgaccag caacggcaga    180

ctgacagccc agttcggcat tacccggaag gacagcttcc tgaacatctc cgccagcatt    240

cccagcgacg tgggcatcta tttttgtgcc ggacag                              276


<210> 13
<211> 285
<212> DNA
<213> Homo sapiens


<220> 
<223> TRB variable region, codon-optimized na

<400> 13
aaagctggcg tgacccagac acctagatac ctgatcaaga ccagaggcca gcaagtgacc     60

ctgagctgct ctcctatcag cggccacaga agcgtgtcct ggtatcagca gacacctgga    120

cagggcctgc agttcctgtt cgagtacttc agcgagacac agcggaacaa gggcaacttc    180

cccggcagat tttccggcag acagttcagc aacagccgca gcgagatgaa cgtgtccaca    240

ctggaactgg gcgacagcgc cctgtatctg tgtgcctctt ctctt                    285


<210> 14
<211> 317
<212> PRT
<213> Homo sapiens


<220> 
<223> MAGEA4, aa

<400> 14
Met Ser Ser Glu Gln Lys Ser Gln His Cys Lys Pro Glu Glu Gly Val 
1               5                   10                  15      
Glu Ala Gln Glu Glu Ala Leu Gly Leu Val Gly Ala Gln Ala Pro Thr 
            20                  25                  30          
Thr Glu Glu Gln Glu Ala Ala Val Ser Ser Ser Ser Pro Leu Val Pro 
        35                  40                  45              
Gly Thr Leu Glu Glu Val Pro Ala Ala Glu Ser Ala Gly Pro Pro Gln 
    50                  55                  60                  
Ser Pro Gln Gly Ala Ser Ala Leu Pro Thr Thr Ile Ser Phe Thr Cys 
65                  70                  75                  80  
Trp Arg Gln Pro Asn Glu Gly Ser Ser Ser Gln Glu Glu Glu Gly Pro 
                85                  90                  95      
Ser Thr Ser Pro Asp Ala Glu Ser Leu Phe Arg Glu Ala Leu Ser Asn 
            100                 105                 110         
Lys Val Asp Glu Leu Ala His Phe Leu Leu Arg Lys Tyr Arg Ala Lys 
        115                 120                 125             
Glu Leu Val Thr Lys Ala Glu Met Leu Glu Arg Val Ile Lys Asn Tyr 
    130                 135                 140                 
Lys Arg Cys Phe Pro Val Ile Phe Gly Lys Ala Ser Glu Ser Leu Lys 
145                 150                 155                 160 
Met Ile Phe Gly Ile Asp Val Lys Glu Val Asp Pro Ala Ser Asn Thr 
                165                 170                 175     
Tyr Thr Leu Val Thr Cys Leu Gly Leu Ser Tyr Asp Gly Leu Leu Gly 
            180                 185                 190         
Asn Asn Gln Ile Phe Pro Lys Thr Gly Leu Leu Ile Ile Val Leu Gly 
        195                 200                 205             
Thr Ile Ala Met Glu Gly Asp Ser Ala Ser Glu Glu Glu Ile Trp Glu 
    210                 215                 220                 
Glu Leu Gly Val Met Gly Val Tyr Asp Gly Arg Glu His Thr Val Tyr 
225                 230                 235                 240 
Gly Glu Pro Arg Lys Leu Leu Thr Gln Asp Trp Val Gln Glu Asn Tyr 
                245                 250                 255     
Leu Glu Tyr Arg Gln Val Pro Gly Ser Asn Pro Ala Arg Tyr Glu Phe 
            260                 265                 270         
Leu Trp Gly Pro Arg Ala Leu Ala Glu Thr Ser Tyr Val Lys Val Leu 
        275                 280                 285             
Glu His Val Val Arg Val Asn Ala Arg Val Arg Ile Ala Tyr Pro Ser 
    290                 295                 300                 
Leu Arg Glu Ala Ala Leu Leu Glu Glu Glu Glu Gly Val 
305                 310                 315         

<210> 15
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> MAGEA3 epitope

<400> 15
Glu Val Asp Pro Ile Gly His Leu Tyr 
1               5                   

<210> 16
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> titin epitope

<400> 16
Glu Ser Asp Pro Ile Val Ala Gln Tyr 
1               5                   

<210> 17
<211> 10
<212> PRT
<213> Homo sapiens


<220> 
<223> MAGEA4 peptide

<400> 17
Thr Leu Val Thr Cys Leu Gly Leu Ser Tyr 
1               5                   10  

<210> 18
<211> 10
<212> PRT
<213> Homo sapiens


<220> 
<223> 10mer MAGEA4 epitope pres. on HLA-A*01:01

<400> 18
Lys Glu Val Asp Pro Ala Ser Asn Thr Tyr 
1               5                   10  

<210> 19
<211> 10
<212> PRT
<213> Homo sapiens


<220> 
<223> 10mer MAGEA4 epitope pres. on HLA-A*01:01

<400> 19
Glu Val Asp Pro Ala Ser Asn Thr Tyr Thr 
1               5                   10  

<210> 20
<211> 10
<212> PRT
<213> Homo sapiens


<220> 
<223> MAGEA4 peptide

<400> 20
Leu Thr Gln Asp Trp Val Gln Glu Asn Tyr 
1               5                   10  

<210> 21
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> MAGEA4 peptide

<400> 21
Trp Val Gln Glu Asn Tyr Leu Glu Tyr 
1               5                   

<210> 22
<211> 10
<212> PRT
<213> Homo sapiens


<220> 
<223> MAGEA4 peptide

<400> 22
Asp Trp Val Gln Glu Asn Tyr Leu Glu Tyr 
1               5                   10  

<210> 23
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> MAGEA4 peptide

<400> 23
Thr Gln Asp Trp Val Gln Glu Asn Tyr 
1               5                   

<210> 24
<211> 10
<212> PRT
<213> Homo sapiens


<220> 
<223> MAGEA4 peptide

<400> 24
Glu Leu Ala His Phe Leu Leu Arg Lys Tyr 
1               5                   10  

<210> 25
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> MAGEA4 peptide

<400> 25
Leu Val Thr Cys Leu Gly Leu Ser Tyr 
1               5                   

<210> 26
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> MAGEA4 peptide

<400> 26
Thr Thr Glu Glu Gln Glu Ala Ala Val 
1               5                   

<210> 27
<211> 10
<212> PRT
<213> Homo sapiens


<220> 
<223> MAGEA4 peptide

<400> 27
Trp Val Gln Glu Asn Tyr Leu Glu Tyr Arg 
1               5                   10  

