                         SEQUENCE LISTING

<110>	 KWS SAAT SE & Co. KGaA
	 Keygene N.V.
	 
<120>    Sample Preparation with Oppositely Oriented Guide Polynucleotides 

<130>	 p6110437pct

<150>    EP 21174714.2
<151>    2021-05-19

<160>    6

<170>  PatentIn version 3.5

<210>    1
<211>    860
<212>    DNA
<213>    Escherichia coli

<220>	
<223>    pC15-1a blaTEM gene highlighted in figure 9


<400>    1

tgagtattca acattttcgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg      60

tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac     120

gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg     180

aagaacgttt tccaatgatg agcactttta aagttctgct atgtggtgcg gtattatccc     240

gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg     300

ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat     360

gcagtgctgc cataaccatg agtgataaca ctgctgccaa cttacttctg acaacgatcg     420

gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg     480

atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc     540

ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt     600

cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct     660

cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc     720

gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca     780

cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct     840

cactgattaa gcattggtaa                                                 860



<210>    2
<211>    1061
<212>    DNA
<213>    Escherichia coli

<220>	
<223>    complete sequence depicted in figure 9 (NG_050145.1) comprising SEQ ID NO 1 at positions 102-961


<400>    2

aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata      60

accctggtaa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacattttcg     120

tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac     180

gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact     240

ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat     300

gagcactttt aaagttctgc tatgtggtgc ggtattatcc cgtgttgacg ccgggcaaga     360

gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac     420

agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat     480

gagtgataac actgctgcca acttacttct gacaacgatc ggaggaccga aggagctaac     540

cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct     600

gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac     660

gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga     720

ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg     780

gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact     840

ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac     900

tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta     960

actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt    1020

taaaaggatc taggtgaaga tcctttttga taatctcatg a                        1061





<210>    3
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>	
<223>    Cas9 crRNA binding sequence for TEM-1 gene


<400>    3
tgtaactcgc cttgatcgtt 							20



<210>    4
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>	
<223>    Cas9 crRNA binding sequence for TEM-1 gene


<400>    4
catacactat tctcagaatg 							20



<210>    5
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>	
<223>    Cas12a crRNA binding sequence for TEM-1 gene


<400>    5
cacaacatgg gggatcatgt a 						21



<210>    6
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>	
<223>    Cas12a crRNA binding sequence for TEM-1 gene


<400>    6
gttgagtactcaccagtcaca  							21







