                         SEQUENCE LISTING

<110>  ѧ
 
<120>  ڼ༭༭λķԼ

<130>  IEC210107PCT

<150>  CN202110551156.9
<151>  2021-05-20

<160>  38    

<170>  PatentIn version 3.5

<210>  1
<211>  137
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Controlģʽ

<400>  1
aactgattgc ccgtctccgc tcgctgggtg aacaactgaa ccgtgatgtc agcatgacgt       60

tatctggcgg tggagatggc tccgtgtggc agagctgaaa gaggagcttg atgacacgta      120

atgcttgcgt ggcaaac                                                     137


<210>  2
<211>  137
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dU:dA-1ģʽ


<220>
<221>  misc_feature
<222>  (48)..(48)
<223>  nΪǺ

<400>  2
aactgattgc ccgtctccgc tcgctgggtg aacaactgaa ccgtgatntc agcatgacgg       60

cggtaagcac gaactcaggc tccgtgtggc agagctgaaa gaggagcttg atgacacggg      120

aaataccgtg gtgtggc                                                     137


<210>  3
<211>  139
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dU:dA-2ģʽ


<220>
<221>  misc_feature
<222>  (48)..(48)
<223>  nΪǺ

<400>  3
aactgattgc ccgtctccgc tcgctgggtg aacaactgaa ccgtgatntc agcatgacgc       60

atgagtgccc tcagcagtag ctccgtgtgg cagagctgaa agaggagctt gatgacacgt      120

ccaaccttta ggagccatg                                                   139


<210>  4
<211>  139
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AP:dAģʽ


<220>
<221>  misc_feature
<222>  (48)..(48)
<223>  nΪAPλ

<400>  4
aactgattgc ccgtctccgc tcgctgggtg aacaactgaa ccgtgatntc agcatgacgc       60

atgagtgccc tcagcagtag ctccgtgtgg cagagctgaa agaggagctt gatgacacgt      120

ccaaccttta ggagccatg                                                   139


<210>  5
<211>  139
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dU:dGģʽ


<220>
<221>  misc_feature
<222>  (48)..(48)
<223>  nΪǺ

<400>  5
aactgattgc ccgtctccgc tcgctgggtg aacaactgaa ccgtgatntc agcatgacgg       60

cggctggagc ggtaattttg ctccgtgtgg cagagctgaa agaggagctt gatgacacgt      120

aatgacgttg ccagccagt                                                   139


<210>  6
<211>  145
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  d5fC:dGģʽ


<220>
<221>  misc_feature
<222>  (100)..(100)
<223>  nΪ5-ȩǺ

<400>  6
catgagtgcc ctcagcagta agtaactgac cagatctctc gtgcctcttg aggctactga       60

gttatccaac ctttaggagc catgcatcga tagcatccgn cacaggcagt gaggctactg      120

agtcatgcac gcagaaagaa atagc                                            145


<210>  7
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Yadaptor

<400>  7
gatcggaaga gcacacgtct gaactccagt c                                      31


<210>  8
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Yadaptor

<400>  8
acactctttc cctacacgac gctcttccga tct                                    33


<210>  9
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Universal Primer

<400>  9
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tccgatct         58


<210>  10
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Index Primer


<220>
<221>  misc_feature
<222>  (25)..(30)
<223>  nԶѡǺᡢǺᡢ
       ǺǺ

<400>  10
caagcagaag acggcatacg agatnnnnnn gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  11
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ControlģʽqPCR-1

<400>  11
ttatctggcg gtggagatg                                                    19


<210>  12
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ControlģʽqPCR-2

<400>  12
gtttgccacg caagcatta                                                    19


<210>  13
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dU:dA-1ģʽqPCR-1

<400>  13
gcggtaagca cgaactcag                                                    19


<210>  14
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dU:dA-1ģʽqPCR-2

<400>  14
gccacaccac ggtatttcc                                                    19


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dU:dA-2ģʽqPCR-1

<400>  15
catgagtgcc ctcagcagta                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dU:dA-2ģʽqPCR-2

<400>  16
catggctcct aaaggttgga                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AP:dAģʽqPCR-1

<400>  17
catgagtgcc ctcagcagta                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AP:dAģʽqPCR-2

<400>  18
catggctcct aaaggttgga                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dU:dGģʽqPCR-1

<400>  19
gcggctggag cggtaatttt                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dU:dGģʽqPCR-2

<400>  20
actggctggc aacgtcatta                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  d5fC:dGģʽqPCR-1

<400>  21
catgagtgcc ctcagcagta                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  d5fC:dGģʽqPCR-2

<400>  22
catggctcct aaaggttgga                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VEGFA_site_2 sgRNAλ

<400>  23
gaccccctcc accccgcctc                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HEK293 site_4 sgRNAλ

<400>  24
ggcactgcgg ctggaggtgg                                                   20


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EMX1 sgRNAλ

<400>  25
gagtccgagc agaagaagaa                                                   20


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RNF2 sgRNAλ

<400>  26
gtcatcttag tcattacctg                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RUNX1 sgRNAλ

<400>  27
tcccctctgc tggatacctc                                                   20


<210>  28
<211>  137
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dI:dCģʽ


<220>
<221>  misc_feature
<222>  (52)..(52)
<223>  nΪλǺ

<400>  28
aactgattgc ccgtctccgc tcgctgggtg aacaactgaa ccgtgatttc ancatgacga       60

atgtggatgc cgcagttggc tccgtgtggc agagctgaaa gaggagcttg atgacacgca      120

accgggacat cacggat                                                     137


<210>  29
<211>  139
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dI:dTģʽ


<220>
<221>  misc_feature
<222>  (40)..(40)
<223>  nΪλǺ

<400>  29
aactgattgc ccgtctccgc tcgctgggtg aacaactgan ccgtgatgtc agcatgacgc       60

tacgcaaact ggctgtcaag ctccgtgtgg cagagctgaa agaggagctt gatgacacgt      120

catggacgct acctcacag                                                   139


<210>  30
<211>  139
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nickģʽ

<400>  30
aactgattgc ccgtctccgc tcgctgggtg aacaactgaa ccgtgatgtc agcatgacga       60

ggccaacata catgccttcg ctccgtgtgg cagagctgaa agaggagctt gatgacacgg      120

aatggcagag tcaaggagc                                                   139


<210>  31
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dI:dCģʽqPCR-1

<400>  31
aatgtggatg ccgcagttg                                                    19


<210>  32
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dI:dCģʽqPCR-2

<400>  32
atccgtgatg tcccggttg                                                    19


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dI:dTģʽqPCR-1

<400>  33
ctacgcaaac tggctgtcaa                                                   20


<210>  34
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dI:dTģʽqPCR-2

<400>  34
ctgtgaggta gcgtccatga                                                   20


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NickģʽqPCR-1

<400>  35
aggccaacat acatgccttc                                                   20


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NickģʽqPCR-2

<400>  36
gctccttgac tctgccattc                                                   20


<210>  37
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RUNX1 crRNAλ

<400>  37
ttctcccctc tgctggatac ctc                                               23


<210>  38
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DYRK1A crRNAλ

<400>  38
gaagcacatc aaggacattc taa                                               23


