                         SEQUENCE LISTING

<110>  Seattle Children's Hospital d/b/a Seattle Children's 
       Research Institute
 
<120>  ACTIVITY-INDUCIBLE FUSION PROTEINS HAVING A TRANSCRIPTION FACTOR 
       AND A HEAT SHOCK PROTEIN 90 BINDING DOMAIN

<130>  S281-0030PCT/SCRI.368WO

<150>  US 63/189,000
<151>  2021-05-14

<160>  184   

<170>  PatentIn version 3.5

<210>  1
<211>  595
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Thr Met Thr Leu His Thr Lys Ala Ser Gly Met Ala Leu Leu His 
1               5                   10                  15      


Gln Ile Gln Gly Asn Glu Leu Glu Pro Leu Asn Arg Pro Gln Leu Lys 
            20                  25                  30          


Ile Pro Leu Glu Arg Pro Leu Gly Glu Val Tyr Leu Asp Ser Ser Lys 
        35                  40                  45              


Pro Ala Val Tyr Asn Tyr Pro Glu Gly Ala Ala Tyr Glu Phe Asn Ala 
    50                  55                  60                  


Ala Ala Ala Ala Asn Ala Gln Val Tyr Gly Gln Thr Gly Leu Pro Tyr 
65                  70                  75                  80  


Gly Pro Gly Ser Glu Ala Ala Ala Phe Gly Ser Asn Gly Leu Gly Gly 
                85                  90                  95      


Phe Pro Pro Leu Asn Ser Val Ser Pro Ser Pro Leu Met Leu Leu His 
            100                 105                 110         


Pro Pro Pro Gln Leu Ser Pro Phe Leu Gln Pro His Gly Gln Gln Val 
        115                 120                 125             


Pro Tyr Tyr Leu Glu Asn Glu Pro Ser Gly Tyr Thr Val Arg Glu Ala 
    130                 135                 140                 


Gly Pro Pro Ala Phe Tyr Arg Pro Asn Ser Asp Asn Arg Arg Gln Gly 
145                 150                 155                 160 


Gly Arg Glu Arg Leu Ala Ser Thr Asn Asp Lys Gly Ser Met Ala Met 
                165                 170                 175     


Glu Ser Ala Lys Glu Thr Arg Tyr Cys Ala Val Cys Asn Asp Tyr Ala 
            180                 185                 190         


Ser Gly Tyr His Tyr Gly Val Trp Ser Cys Glu Gly Cys Lys Ala Phe 
        195                 200                 205             


Phe Lys Arg Ser Ile Gln Gly His Asn Asp Tyr Met Cys Pro Ala Thr 
    210                 215                 220                 


Asn Gln Cys Thr Ile Asp Lys Asn Arg Arg Lys Ser Cys Gln Ala Cys 
225                 230                 235                 240 


Arg Leu Arg Lys Cys Tyr Glu Val Gly Met Met Lys Gly Gly Ile Arg 
                245                 250                 255     


Lys Asp Arg Arg Gly Gly Arg Met Leu Lys His Lys Arg Gln Arg Asp 
            260                 265                 270         


Asp Gly Glu Gly Arg Gly Glu Val Gly Ser Ala Gly Asp Met Arg Ala 
        275                 280                 285             


Ala Asn Leu Trp Pro Ser Pro Leu Met Ile Lys Arg Ser Lys Lys Asn 
    290                 295                 300                 


Ser Leu Ala Leu Ser Leu Thr Ala Asp Gln Met Val Ser Ala Leu Leu 
305                 310                 315                 320 


Asp Ala Glu Pro Pro Ile Leu Tyr Ser Glu Tyr Asp Pro Thr Arg Pro 
                325                 330                 335     


Phe Ser Glu Ala Ser Met Met Gly Leu Leu Thr Asn Leu Ala Asp Arg 
            340                 345                 350         


Glu Leu Val His Met Ile Asn Trp Ala Lys Arg Val Pro Gly Phe Val 
        355                 360                 365             


Asp Leu Thr Leu His Asp Gln Val His Leu Leu Glu Cys Ala Trp Leu 
    370                 375                 380                 


Glu Ile Leu Met Ile Gly Leu Val Trp Arg Ser Met Glu His Pro Gly 
385                 390                 395                 400 


Lys Leu Leu Phe Ala Pro Asn Leu Leu Leu Asp Arg Asn Gln Gly Lys 
                405                 410                 415     


Cys Val Glu Gly Met Val Glu Ile Phe Asp Met Leu Leu Ala Thr Ser 
            420                 425                 430         


Ser Arg Phe Arg Met Met Asn Leu Gln Gly Glu Glu Phe Val Cys Leu 
        435                 440                 445             


Lys Ser Ile Ile Leu Leu Asn Ser Gly Val Tyr Thr Phe Leu Ser Ser 
    450                 455                 460                 


Thr Leu Lys Ser Leu Glu Glu Lys Asp His Ile His Arg Val Leu Asp 
465                 470                 475                 480 


Lys Ile Thr Asp Thr Leu Ile His Leu Met Ala Lys Ala Gly Leu Thr 
                485                 490                 495     


Leu Gln Gln Gln His Gln Arg Leu Ala Gln Leu Leu Leu Ile Leu Ser 
            500                 505                 510         


His Ile Arg His Met Ser Asn Lys Gly Met Glu His Leu Tyr Ser Met 
        515                 520                 525             


Lys Cys Lys Asn Val Val Pro Leu Tyr Asp Leu Leu Leu Glu Met Leu 
    530                 535                 540                 


Asp Ala His Arg Leu His Ala Pro Thr Ser Arg Gly Gly Ala Ser Val 
545                 550                 555                 560 


Glu Glu Thr Asp Gln Ser His Leu Ala Thr Ala Gly Ser Thr Ser Ser 
                565                 570                 575     


His Ser Leu Gln Lys Tyr Tyr Ile Thr Gly Glu Ala Glu Gly Phe Pro 
            580                 585                 590         


Ala Thr Val 
        595 


<210>  2
<211>  314
<212>  PRT
<213>  Homo sapiens

<400>  2

Ser Ala Gly Asp Met Arg Ala Ala Asn Leu Trp Pro Ser Pro Leu Met 
1               5                   10                  15      


Ile Lys Arg Ser Lys Lys Asn Ser Leu Ala Leu Ser Leu Thr Ala Asp 
            20                  25                  30          


Gln Met Val Ser Ala Leu Leu Asp Ala Glu Pro Pro Ile Leu Tyr Ser 
        35                  40                  45              


Glu Tyr Asp Pro Thr Arg Pro Phe Ser Glu Ala Ser Met Met Gly Leu 
    50                  55                  60                  


Leu Thr Asn Leu Ala Asp Arg Glu Leu Val His Met Ile Asn Trp Ala 
65                  70                  75                  80  


Lys Arg Val Pro Gly Phe Val Asp Leu Thr Leu His Asp Gln Val His 
                85                  90                  95      


Leu Leu Glu Cys Ala Trp Leu Glu Ile Leu Met Ile Gly Leu Val Trp 
            100                 105                 110         


Arg Ser Met Glu His Pro Gly Lys Leu Leu Phe Ala Pro Asn Leu Leu 
        115                 120                 125             


Leu Asp Arg Asn Gln Gly Lys Cys Val Glu Gly Met Val Glu Ile Phe 
    130                 135                 140                 


Asp Met Leu Leu Ala Thr Ser Ser Arg Phe Arg Met Met Asn Leu Gln 
145                 150                 155                 160 


Gly Glu Glu Phe Val Cys Leu Lys Ser Ile Ile Leu Leu Asn Ser Gly 
                165                 170                 175     


Val Tyr Thr Phe Leu Ser Ser Thr Leu Lys Ser Leu Glu Glu Lys Asp 
            180                 185                 190         


His Ile His Arg Val Leu Asp Lys Ile Thr Asp Thr Leu Ile His Leu 
        195                 200                 205             


Met Ala Lys Ala Gly Leu Thr Leu Gln Gln Gln His Gln Arg Leu Ala 
    210                 215                 220                 


Gln Leu Leu Leu Ile Leu Ser His Ile Arg His Met Ser Asn Lys Gly 
225                 230                 235                 240 


Met Glu His Leu Tyr Ser Met Lys Cys Lys Asn Val Val Pro Leu Tyr 
                245                 250                 255     


Asp Leu Leu Leu Glu Met Leu Asp Ala His Arg Leu His Ala Pro Thr 
            260                 265                 270         


Ser Arg Gly Gly Ala Ser Val Glu Glu Thr Asp Gln Ser His Leu Ala 
        275                 280                 285             


Thr Ala Gly Ser Thr Ser Ser His Ser Leu Gln Lys Tyr Tyr Ile Thr 
    290                 295                 300                 


Gly Glu Ala Glu Gly Phe Pro Ala Thr Val 
305                 310                 


<210>  3
<211>  595
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Estrogen Receptor with G521R mutation

<400>  3

Met Thr Met Thr Leu His Thr Lys Ala Ser Gly Met Ala Leu Leu His 
1               5                   10                  15      


Gln Ile Gln Gly Asn Glu Leu Glu Pro Leu Asn Arg Pro Gln Leu Lys 
            20                  25                  30          


Ile Pro Leu Glu Arg Pro Leu Gly Glu Val Tyr Leu Asp Ser Ser Lys 
        35                  40                  45              


Pro Ala Val Tyr Asn Tyr Pro Glu Gly Ala Ala Tyr Glu Phe Asn Ala 
    50                  55                  60                  


Ala Ala Ala Ala Asn Ala Gln Val Tyr Gly Gln Thr Gly Leu Pro Tyr 
65                  70                  75                  80  


Gly Pro Gly Ser Glu Ala Ala Ala Phe Gly Ser Asn Gly Leu Gly Gly 
                85                  90                  95      


Phe Pro Pro Leu Asn Ser Val Ser Pro Ser Pro Leu Met Leu Leu His 
            100                 105                 110         


Pro Pro Pro Gln Leu Ser Pro Phe Leu Gln Pro His Gly Gln Gln Val 
        115                 120                 125             


Pro Tyr Tyr Leu Glu Asn Glu Pro Ser Gly Tyr Thr Val Arg Glu Ala 
    130                 135                 140                 


Gly Pro Pro Ala Phe Tyr Arg Pro Asn Ser Asp Asn Arg Arg Gln Gly 
145                 150                 155                 160 


Gly Arg Glu Arg Leu Ala Ser Thr Asn Asp Lys Gly Ser Met Ala Met 
                165                 170                 175     


Glu Ser Ala Lys Glu Thr Arg Tyr Cys Ala Val Cys Asn Asp Tyr Ala 
            180                 185                 190         


Ser Gly Tyr His Tyr Gly Val Trp Ser Cys Glu Gly Cys Lys Ala Phe 
        195                 200                 205             


Phe Lys Arg Ser Ile Gln Gly His Asn Asp Tyr Met Cys Pro Ala Thr 
    210                 215                 220                 


Asn Gln Cys Thr Ile Asp Lys Asn Arg Arg Lys Ser Cys Gln Ala Cys 
225                 230                 235                 240 


Arg Leu Arg Lys Cys Tyr Glu Val Gly Met Met Lys Gly Gly Ile Arg 
                245                 250                 255     


Lys Asp Arg Arg Gly Gly Arg Met Leu Lys His Lys Arg Gln Arg Asp 
            260                 265                 270         


Asp Gly Glu Gly Arg Gly Glu Val Gly Ser Ala Gly Asp Met Arg Ala 
        275                 280                 285             


Ala Asn Leu Trp Pro Ser Pro Leu Met Ile Lys Arg Ser Lys Lys Asn 
    290                 295                 300                 


Ser Leu Ala Leu Ser Leu Thr Ala Asp Gln Met Val Ser Ala Leu Leu 
305                 310                 315                 320 


Asp Ala Glu Pro Pro Ile Leu Tyr Ser Glu Tyr Asp Pro Thr Arg Pro 
                325                 330                 335     


Phe Ser Glu Ala Ser Met Met Gly Leu Leu Thr Asn Leu Ala Asp Arg 
            340                 345                 350         


Glu Leu Val His Met Ile Asn Trp Ala Lys Arg Val Pro Gly Phe Val 
        355                 360                 365             


Asp Leu Thr Leu His Asp Gln Val His Leu Leu Glu Cys Ala Trp Leu 
    370                 375                 380                 


Glu Ile Leu Met Ile Gly Leu Val Trp Arg Ser Met Glu His Pro Gly 
385                 390                 395                 400 


Lys Leu Leu Phe Ala Pro Asn Leu Leu Leu Asp Arg Asn Gln Gly Lys 
                405                 410                 415     


Cys Val Glu Gly Met Val Glu Ile Phe Asp Met Leu Leu Ala Thr Ser 
            420                 425                 430         


Ser Arg Phe Arg Met Met Asn Leu Gln Gly Glu Glu Phe Val Cys Leu 
        435                 440                 445             


Lys Ser Ile Ile Leu Leu Asn Ser Gly Val Tyr Thr Phe Leu Ser Ser 
    450                 455                 460                 


Thr Leu Lys Ser Leu Glu Glu Lys Asp His Ile His Arg Val Leu Asp 
465                 470                 475                 480 


Lys Ile Thr Asp Thr Leu Ile His Leu Met Ala Lys Ala Gly Leu Thr 
                485                 490                 495     


Leu Gln Gln Gln His Gln Arg Leu Ala Gln Leu Leu Leu Ile Leu Ser 
            500                 505                 510         


His Ile Arg His Met Ser Asn Lys Arg Met Glu His Leu Tyr Ser Met 
        515                 520                 525             


Lys Cys Lys Asn Val Val Pro Leu Tyr Asp Leu Leu Leu Glu Met Leu 
    530                 535                 540                 


Asp Ala His Arg Leu His Ala Pro Thr Ser Arg Gly Gly Ala Ser Val 
545                 550                 555                 560 


Glu Glu Thr Asp Gln Ser His Leu Ala Thr Ala Gly Ser Thr Ser Ser 
                565                 570                 575     


His Ser Leu Gln Lys Tyr Tyr Ile Thr Gly Glu Ala Glu Gly Phe Pro 
            580                 585                 590         


Ala Thr Val 
        595 


<210>  4
<211>  314
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EBD with G521R mutation

<400>  4

Ser Ala Gly Asp Met Arg Ala Ala Asn Leu Trp Pro Ser Pro Leu Met 
1               5                   10                  15      


Ile Lys Arg Ser Lys Lys Asn Ser Leu Ala Leu Ser Leu Thr Ala Asp 
            20                  25                  30          


Gln Met Val Ser Ala Leu Leu Asp Ala Glu Pro Pro Ile Leu Tyr Ser 
        35                  40                  45              


Glu Tyr Asp Pro Thr Arg Pro Phe Ser Glu Ala Ser Met Met Gly Leu 
    50                  55                  60                  


Leu Thr Asn Leu Ala Asp Arg Glu Leu Val His Met Ile Asn Trp Ala 
65                  70                  75                  80  


Lys Arg Val Pro Gly Phe Val Asp Leu Thr Leu His Asp Gln Val His 
                85                  90                  95      


Leu Leu Glu Cys Ala Trp Leu Glu Ile Leu Met Ile Gly Leu Val Trp 
            100                 105                 110         


Arg Ser Met Glu His Pro Gly Lys Leu Leu Phe Ala Pro Asn Leu Leu 
        115                 120                 125             


Leu Asp Arg Asn Gln Gly Lys Cys Val Glu Gly Met Val Glu Ile Phe 
    130                 135                 140                 


Asp Met Leu Leu Ala Thr Ser Ser Arg Phe Arg Met Met Asn Leu Gln 
145                 150                 155                 160 


Gly Glu Glu Phe Val Cys Leu Lys Ser Ile Ile Leu Leu Asn Ser Gly 
                165                 170                 175     


Val Tyr Thr Phe Leu Ser Ser Thr Leu Lys Ser Leu Glu Glu Lys Asp 
            180                 185                 190         


His Ile His Arg Val Leu Asp Lys Ile Thr Asp Thr Leu Ile His Leu 
        195                 200                 205             


Met Ala Lys Ala Gly Leu Thr Leu Gln Gln Gln His Gln Arg Leu Ala 
    210                 215                 220                 


Gln Leu Leu Leu Ile Leu Ser His Ile Arg His Met Ser Asn Lys Arg 
225                 230                 235                 240 


Met Glu His Leu Tyr Ser Met Lys Cys Lys Asn Val Val Pro Leu Tyr 
                245                 250                 255     


Asp Leu Leu Leu Glu Met Leu Asp Ala His Arg Leu His Ala Pro Thr 
            260                 265                 270         


Ser Arg Gly Gly Ala Ser Val Glu Glu Thr Asp Gln Ser His Leu Ala 
        275                 280                 285             


Thr Ala Gly Ser Thr Ser Ser His Ser Leu Gln Lys Tyr Tyr Ile Thr 
    290                 295                 300                 


Gly Glu Ala Glu Gly Phe Pro Ala Thr Val 
305                 310                 


<210>  5
<211>  595
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Estrogen Receptor with E353A mutation

<400>  5

Met Thr Met Thr Leu His Thr Lys Ala Ser Gly Met Ala Leu Leu His 
1               5                   10                  15      


Gln Ile Gln Gly Asn Glu Leu Glu Pro Leu Asn Arg Pro Gln Leu Lys 
            20                  25                  30          


Ile Pro Leu Glu Arg Pro Leu Gly Glu Val Tyr Leu Asp Ser Ser Lys 
        35                  40                  45              


Pro Ala Val Tyr Asn Tyr Pro Glu Gly Ala Ala Tyr Glu Phe Asn Ala 
    50                  55                  60                  


Ala Ala Ala Ala Asn Ala Gln Val Tyr Gly Gln Thr Gly Leu Pro Tyr 
65                  70                  75                  80  


Gly Pro Gly Ser Glu Ala Ala Ala Phe Gly Ser Asn Gly Leu Gly Gly 
                85                  90                  95      


Phe Pro Pro Leu Asn Ser Val Ser Pro Ser Pro Leu Met Leu Leu His 
            100                 105                 110         


Pro Pro Pro Gln Leu Ser Pro Phe Leu Gln Pro His Gly Gln Gln Val 
        115                 120                 125             


Pro Tyr Tyr Leu Glu Asn Glu Pro Ser Gly Tyr Thr Val Arg Glu Ala 
    130                 135                 140                 


Gly Pro Pro Ala Phe Tyr Arg Pro Asn Ser Asp Asn Arg Arg Gln Gly 
145                 150                 155                 160 


Gly Arg Glu Arg Leu Ala Ser Thr Asn Asp Lys Gly Ser Met Ala Met 
                165                 170                 175     


Glu Ser Ala Lys Glu Thr Arg Tyr Cys Ala Val Cys Asn Asp Tyr Ala 
            180                 185                 190         


Ser Gly Tyr His Tyr Gly Val Trp Ser Cys Glu Gly Cys Lys Ala Phe 
        195                 200                 205             


Phe Lys Arg Ser Ile Gln Gly His Asn Asp Tyr Met Cys Pro Ala Thr 
    210                 215                 220                 


Asn Gln Cys Thr Ile Asp Lys Asn Arg Arg Lys Ser Cys Gln Ala Cys 
225                 230                 235                 240 


Arg Leu Arg Lys Cys Tyr Glu Val Gly Met Met Lys Gly Gly Ile Arg 
                245                 250                 255     


Lys Asp Arg Arg Gly Gly Arg Met Leu Lys His Lys Arg Gln Arg Asp 
            260                 265                 270         


Asp Gly Glu Gly Arg Gly Glu Val Gly Ser Ala Gly Asp Met Arg Ala 
        275                 280                 285             


Ala Asn Leu Trp Pro Ser Pro Leu Met Ile Lys Arg Ser Lys Lys Asn 
    290                 295                 300                 


Ser Leu Ala Leu Ser Leu Thr Ala Asp Gln Met Val Ser Ala Leu Leu 
305                 310                 315                 320 


Asp Ala Glu Pro Pro Ile Leu Tyr Ser Glu Tyr Asp Pro Thr Arg Pro 
                325                 330                 335     


Phe Ser Glu Ala Ser Met Met Gly Leu Leu Thr Asn Leu Ala Asp Arg 
            340                 345                 350         


Ala Leu Val His Met Ile Asn Trp Ala Lys Arg Val Pro Gly Phe Val 
        355                 360                 365             


Asp Leu Thr Leu His Asp Gln Val His Leu Leu Glu Cys Ala Trp Leu 
    370                 375                 380                 


Glu Ile Leu Met Ile Gly Leu Val Trp Arg Ser Met Glu His Pro Gly 
385                 390                 395                 400 


Lys Leu Leu Phe Ala Pro Asn Leu Leu Leu Asp Arg Asn Gln Gly Lys 
                405                 410                 415     


Cys Val Glu Gly Met Val Glu Ile Phe Asp Met Leu Leu Ala Thr Ser 
            420                 425                 430         


Ser Arg Phe Arg Met Met Asn Leu Gln Gly Glu Glu Phe Val Cys Leu 
        435                 440                 445             


Lys Ser Ile Ile Leu Leu Asn Ser Gly Val Tyr Thr Phe Leu Ser Ser 
    450                 455                 460                 


Thr Leu Lys Ser Leu Glu Glu Lys Asp His Ile His Arg Val Leu Asp 
465                 470                 475                 480 


Lys Ile Thr Asp Thr Leu Ile His Leu Met Ala Lys Ala Gly Leu Thr 
                485                 490                 495     


Leu Gln Gln Gln His Gln Arg Leu Ala Gln Leu Leu Leu Ile Leu Ser 
            500                 505                 510         


His Ile Arg His Met Ser Asn Lys Gly Met Glu His Leu Tyr Ser Met 
        515                 520                 525             


Lys Cys Lys Asn Val Val Pro Leu Tyr Asp Leu Leu Leu Glu Met Leu 
    530                 535                 540                 


Asp Ala His Arg Leu His Ala Pro Thr Ser Arg Gly Gly Ala Ser Val 
545                 550                 555                 560 


Glu Glu Thr Asp Gln Ser His Leu Ala Thr Ala Gly Ser Thr Ser Ser 
                565                 570                 575     


His Ser Leu Gln Lys Tyr Tyr Ile Thr Gly Glu Ala Glu Gly Phe Pro 
            580                 585                 590         


Ala Thr Val 
        595 


<210>  6
<211>  314
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EBD (E353A) Pairs with estrogen analog ES8

<400>  6

Ser Ala Gly Asp Met Arg Ala Ala Asn Leu Trp Pro Ser Pro Leu Met 
1               5                   10                  15      


Ile Lys Arg Ser Lys Lys Asn Ser Leu Ala Leu Ser Leu Thr Ala Asp 
            20                  25                  30          


Gln Met Val Ser Ala Leu Leu Asp Ala Glu Pro Pro Ile Leu Tyr Ser 
        35                  40                  45              


Glu Tyr Asp Pro Thr Arg Pro Phe Ser Glu Ala Ser Met Met Gly Leu 
    50                  55                  60                  


Leu Thr Asn Leu Ala Asp Arg Ala Leu Val His Met Ile Asn Trp Ala 
65                  70                  75                  80  


Lys Arg Val Pro Gly Phe Val Asp Leu Thr Leu His Asp Gln Val His 
                85                  90                  95      


Leu Leu Glu Cys Ala Trp Leu Glu Ile Leu Met Ile Gly Leu Val Trp 
            100                 105                 110         


Arg Ser Met Glu His Pro Gly Lys Leu Leu Phe Ala Pro Asn Leu Leu 
        115                 120                 125             


Leu Asp Arg Asn Gln Gly Lys Cys Val Glu Gly Met Val Glu Ile Phe 
    130                 135                 140                 


Asp Met Leu Leu Ala Thr Ser Ser Arg Phe Arg Met Met Asn Leu Gln 
145                 150                 155                 160 


Gly Glu Glu Phe Val Cys Leu Lys Ser Ile Ile Leu Leu Asn Ser Gly 
                165                 170                 175     


Val Tyr Thr Phe Leu Ser Ser Thr Leu Lys Ser Leu Glu Glu Lys Asp 
            180                 185                 190         


His Ile His Arg Val Leu Asp Lys Ile Thr Asp Thr Leu Ile His Leu 
        195                 200                 205             


Met Ala Lys Ala Gly Leu Thr Leu Gln Gln Gln His Gln Arg Leu Ala 
    210                 215                 220                 


Gln Leu Leu Leu Ile Leu Ser His Ile Arg His Met Ser Asn Lys Gly 
225                 230                 235                 240 


Met Glu His Leu Tyr Ser Met Lys Cys Lys Asn Val Val Pro Leu Tyr 
                245                 250                 255     


Asp Leu Leu Leu Glu Met Leu Asp Ala His Arg Leu His Ala Pro Thr 
            260                 265                 270         


Ser Arg Gly Gly Ala Ser Val Glu Glu Thr Asp Gln Ser His Leu Ala 
        275                 280                 285             


Thr Ala Gly Ser Thr Ser Ser His Ser Leu Gln Lys Tyr Tyr Ile Thr 
    290                 295                 300                 


Gly Glu Ala Glu Gly Phe Pro Ala Thr Val 
305                 310                 


<210>  7
<211>  942
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EBD (E353A) Coding Sequence

<400>  7
tctgctggcg atatgagagc cgctaacctg tggccttctc cactgatgat caagcggagc       60

aagaagaact ctctggccct gtctctgacc gccgaccaga tggtttctgc tctgctggat      120

gccgagcctc ctatcctgta ctccgagtac gaccccacca gaccttttag cgaggccagc      180

atgatgggcc tgctgaccaa tctggctgac agagccctgg tgcacatgat caactgggcc      240

aagcgcgtgc ccggctttgt cgatctgaca ctgcacgacc aagtgcatct gctggaatgc      300

gcctggctgg aaattctgat gatcggcctc gtgtggcgga gcatggaaca ccctggaaaa      360

ctgctgttcg cccctaacct gctgctcgac agaaaccagg gcaaatgcgt ggaaggcatg      420

gtggaaatct tcgatatgct gctggccacc tccagccggt tccggatgat gaatctccag      480

ggcgaagagt tcgtgtgcct gaagtccatc atcctgctga atagcggcgt gtacacattc      540

ctgagcagca ccctgaaatc cctggaagag aaggaccaca tccaccgggt gctcgacaag      600

atcaccgaca cactgatcca cctgatggcc aaggccggac tgactctgca gcagcaacat      660

cagagactgg cccagctgct gctcatcctg agccacatca gacacatgag caacaaaggc      720

atggaacatc tgtacagcat gaagtgcaag aacgtggtgc ccctgtacga cctgctcctg      780

gaaatgctgg acgcccacag actgcacgcc cctacatcta gaggtggcgc ctctgtggaa      840

gagacagacc agtctcacct ggccacagcc ggcagcacaa gctctcattc cctgcagaag      900

tactacatca ccggcgaggc cgagggattt cctgccacag tt                         942


<210>  8
<211>  595
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Estrogen Receptor with L384M and M421G mutations

<400>  8

Met Thr Met Thr Leu His Thr Lys Ala Ser Gly Met Ala Leu Leu His 
1               5                   10                  15      


Gln Ile Gln Gly Asn Glu Leu Glu Pro Leu Asn Arg Pro Gln Leu Lys 
            20                  25                  30          


Ile Pro Leu Glu Arg Pro Leu Gly Glu Val Tyr Leu Asp Ser Ser Lys 
        35                  40                  45              


Pro Ala Val Tyr Asn Tyr Pro Glu Gly Ala Ala Tyr Glu Phe Asn Ala 
    50                  55                  60                  


Ala Ala Ala Ala Asn Ala Gln Val Tyr Gly Gln Thr Gly Leu Pro Tyr 
65                  70                  75                  80  


Gly Pro Gly Ser Glu Ala Ala Ala Phe Gly Ser Asn Gly Leu Gly Gly 
                85                  90                  95      


Phe Pro Pro Leu Asn Ser Val Ser Pro Ser Pro Leu Met Leu Leu His 
            100                 105                 110         


Pro Pro Pro Gln Leu Ser Pro Phe Leu Gln Pro His Gly Gln Gln Val 
        115                 120                 125             


Pro Tyr Tyr Leu Glu Asn Glu Pro Ser Gly Tyr Thr Val Arg Glu Ala 
    130                 135                 140                 


Gly Pro Pro Ala Phe Tyr Arg Pro Asn Ser Asp Asn Arg Arg Gln Gly 
145                 150                 155                 160 


Gly Arg Glu Arg Leu Ala Ser Thr Asn Asp Lys Gly Ser Met Ala Met 
                165                 170                 175     


Glu Ser Ala Lys Glu Thr Arg Tyr Cys Ala Val Cys Asn Asp Tyr Ala 
            180                 185                 190         


Ser Gly Tyr His Tyr Gly Val Trp Ser Cys Glu Gly Cys Lys Ala Phe 
        195                 200                 205             


Phe Lys Arg Ser Ile Gln Gly His Asn Asp Tyr Met Cys Pro Ala Thr 
    210                 215                 220                 


Asn Gln Cys Thr Ile Asp Lys Asn Arg Arg Lys Ser Cys Gln Ala Cys 
225                 230                 235                 240 


Arg Leu Arg Lys Cys Tyr Glu Val Gly Met Met Lys Gly Gly Ile Arg 
                245                 250                 255     


Lys Asp Arg Arg Gly Gly Arg Met Leu Lys His Lys Arg Gln Arg Asp 
            260                 265                 270         


Asp Gly Glu Gly Arg Gly Glu Val Gly Ser Ala Gly Asp Met Arg Ala 
        275                 280                 285             


Ala Asn Leu Trp Pro Ser Pro Leu Met Ile Lys Arg Ser Lys Lys Asn 
    290                 295                 300                 


Ser Leu Ala Leu Ser Leu Thr Ala Asp Gln Met Val Ser Ala Leu Leu 
305                 310                 315                 320 


Asp Ala Glu Pro Pro Ile Leu Tyr Ser Glu Tyr Asp Pro Thr Arg Pro 
                325                 330                 335     


Phe Ser Glu Ala Ser Met Met Gly Leu Leu Thr Asn Leu Ala Asp Arg 
            340                 345                 350         


Glu Leu Val His Met Ile Asn Trp Ala Lys Arg Val Pro Gly Phe Val 
        355                 360                 365             


Asp Leu Thr Leu His Asp Gln Val His Leu Leu Glu Cys Ala Trp Met 
    370                 375                 380                 


Glu Ile Leu Met Ile Gly Leu Val Trp Arg Ser Met Glu His Pro Gly 
385                 390                 395                 400 


Lys Leu Leu Phe Ala Pro Asn Leu Leu Leu Asp Arg Asn Gln Gly Lys 
                405                 410                 415     


Cys Val Glu Gly Gly Val Glu Ile Phe Asp Met Leu Leu Ala Thr Ser 
            420                 425                 430         


Ser Arg Phe Arg Met Met Asn Leu Gln Gly Glu Glu Phe Val Cys Leu 
        435                 440                 445             


Lys Ser Ile Ile Leu Leu Asn Ser Gly Val Tyr Thr Phe Leu Ser Ser 
    450                 455                 460                 


Thr Leu Lys Ser Leu Glu Glu Lys Asp His Ile His Arg Val Leu Asp 
465                 470                 475                 480 


Lys Ile Thr Asp Thr Leu Ile His Leu Met Ala Lys Ala Gly Leu Thr 
                485                 490                 495     


Leu Gln Gln Gln His Gln Arg Leu Ala Gln Leu Leu Leu Ile Leu Ser 
            500                 505                 510         


His Ile Arg His Met Ser Asn Lys Gly Met Glu His Leu Tyr Ser Met 
        515                 520                 525             


Lys Cys Lys Asn Val Val Pro Leu Tyr Asp Leu Leu Leu Glu Met Leu 
    530                 535                 540                 


Asp Ala His Arg Leu His Ala Pro Thr Ser Arg Gly Gly Ala Ser Val 
545                 550                 555                 560 


Glu Glu Thr Asp Gln Ser His Leu Ala Thr Ala Gly Ser Thr Ser Ser 
                565                 570                 575     


His Ser Leu Gln Lys Tyr Tyr Ile Thr Gly Glu Ala Glu Gly Phe Pro 
            580                 585                 590         


Ala Thr Val 
        595 


<210>  9
<211>  314
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EBD with L384M and M421G mutations

<400>  9

Ser Ala Gly Asp Met Arg Ala Ala Asn Leu Trp Pro Ser Pro Leu Met 
1               5                   10                  15      


Ile Lys Arg Ser Lys Lys Asn Ser Leu Ala Leu Ser Leu Thr Ala Asp 
            20                  25                  30          


Gln Met Val Ser Ala Leu Leu Asp Ala Glu Pro Pro Ile Leu Tyr Ser 
        35                  40                  45              


Glu Tyr Asp Pro Thr Arg Pro Phe Ser Glu Ala Ser Met Met Gly Leu 
    50                  55                  60                  


Leu Thr Asn Leu Ala Asp Arg Glu Leu Val His Met Ile Asn Trp Ala 
65                  70                  75                  80  


Lys Arg Val Pro Gly Phe Val Asp Leu Thr Leu His Asp Gln Val His 
                85                  90                  95      


Leu Leu Glu Cys Ala Trp Met Glu Ile Leu Met Ile Gly Leu Val Trp 
            100                 105                 110         


Arg Ser Met Glu His Pro Gly Lys Leu Leu Phe Ala Pro Asn Leu Leu 
        115                 120                 125             


Leu Asp Arg Asn Gln Gly Lys Cys Val Glu Gly Gly Val Glu Ile Phe 
    130                 135                 140                 


Asp Met Leu Leu Ala Thr Ser Ser Arg Phe Arg Met Met Asn Leu Gln 
145                 150                 155                 160 


Gly Glu Glu Phe Val Cys Leu Lys Ser Ile Ile Leu Leu Asn Ser Gly 
                165                 170                 175     


Val Tyr Thr Phe Leu Ser Ser Thr Leu Lys Ser Leu Glu Glu Lys Asp 
            180                 185                 190         


His Ile His Arg Val Leu Asp Lys Ile Thr Asp Thr Leu Ile His Leu 
        195                 200                 205             


Met Ala Lys Ala Gly Leu Thr Leu Gln Gln Gln His Gln Arg Leu Ala 
    210                 215                 220                 


Gln Leu Leu Leu Ile Leu Ser His Ile Arg His Met Ser Asn Lys Gly 
225                 230                 235                 240 


Met Glu His Leu Tyr Ser Met Lys Cys Lys Asn Val Val Pro Leu Tyr 
                245                 250                 255     


Asp Leu Leu Leu Glu Met Leu Asp Ala His Arg Leu His Ala Pro Thr 
            260                 265                 270         


Ser Arg Gly Gly Ala Ser Val Glu Glu Thr Asp Gln Ser His Leu Ala 
        275                 280                 285             


Thr Ala Gly Ser Thr Ser Ser His Ser Leu Gln Lys Tyr Tyr Ile Thr 
    290                 295                 300                 


Gly Glu Ala Glu Gly Phe Pro Ala Thr Val 
305                 310                 


<210>  10
<211>  595
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Estrogen Receptor with L384M, M421G, and G521R mutations

<400>  10

Met Thr Met Thr Leu His Thr Lys Ala Ser Gly Met Ala Leu Leu His 
1               5                   10                  15      


Gln Ile Gln Gly Asn Glu Leu Glu Pro Leu Asn Arg Pro Gln Leu Lys 
            20                  25                  30          


Ile Pro Leu Glu Arg Pro Leu Gly Glu Val Tyr Leu Asp Ser Ser Lys 
        35                  40                  45              


Pro Ala Val Tyr Asn Tyr Pro Glu Gly Ala Ala Tyr Glu Phe Asn Ala 
    50                  55                  60                  


Ala Ala Ala Ala Asn Ala Gln Val Tyr Gly Gln Thr Gly Leu Pro Tyr 
65                  70                  75                  80  


Gly Pro Gly Ser Glu Ala Ala Ala Phe Gly Ser Asn Gly Leu Gly Gly 
                85                  90                  95      


Phe Pro Pro Leu Asn Ser Val Ser Pro Ser Pro Leu Met Leu Leu His 
            100                 105                 110         


Pro Pro Pro Gln Leu Ser Pro Phe Leu Gln Pro His Gly Gln Gln Val 
        115                 120                 125             


Pro Tyr Tyr Leu Glu Asn Glu Pro Ser Gly Tyr Thr Val Arg Glu Ala 
    130                 135                 140                 


Gly Pro Pro Ala Phe Tyr Arg Pro Asn Ser Asp Asn Arg Arg Gln Gly 
145                 150                 155                 160 


Gly Arg Glu Arg Leu Ala Ser Thr Asn Asp Lys Gly Ser Met Ala Met 
                165                 170                 175     


Glu Ser Ala Lys Glu Thr Arg Tyr Cys Ala Val Cys Asn Asp Tyr Ala 
            180                 185                 190         


Ser Gly Tyr His Tyr Gly Val Trp Ser Cys Glu Gly Cys Lys Ala Phe 
        195                 200                 205             


Phe Lys Arg Ser Ile Gln Gly His Asn Asp Tyr Met Cys Pro Ala Thr 
    210                 215                 220                 


Asn Gln Cys Thr Ile Asp Lys Asn Arg Arg Lys Ser Cys Gln Ala Cys 
225                 230                 235                 240 


Arg Leu Arg Lys Cys Tyr Glu Val Gly Met Met Lys Gly Gly Ile Arg 
                245                 250                 255     


Lys Asp Arg Arg Gly Gly Arg Met Leu Lys His Lys Arg Gln Arg Asp 
            260                 265                 270         


Asp Gly Glu Gly Arg Gly Glu Val Gly Ser Ala Gly Asp Met Arg Ala 
        275                 280                 285             


Ala Asn Leu Trp Pro Ser Pro Leu Met Ile Lys Arg Ser Lys Lys Asn 
    290                 295                 300                 


Ser Leu Ala Leu Ser Leu Thr Ala Asp Gln Met Val Ser Ala Leu Leu 
305                 310                 315                 320 


Asp Ala Glu Pro Pro Ile Leu Tyr Ser Glu Tyr Asp Pro Thr Arg Pro 
                325                 330                 335     


Phe Ser Glu Ala Ser Met Met Gly Leu Leu Thr Asn Leu Ala Asp Arg 
            340                 345                 350         


Glu Leu Val His Met Ile Asn Trp Ala Lys Arg Val Pro Gly Phe Val 
        355                 360                 365             


Asp Leu Thr Leu His Asp Gln Val His Leu Leu Glu Cys Ala Trp Met 
    370                 375                 380                 


Glu Ile Leu Met Ile Gly Leu Val Trp Arg Ser Met Glu His Pro Gly 
385                 390                 395                 400 


Lys Leu Leu Phe Ala Pro Asn Leu Leu Leu Asp Arg Asn Gln Gly Lys 
                405                 410                 415     


Cys Val Glu Gly Gly Val Glu Ile Phe Asp Met Leu Leu Ala Thr Ser 
            420                 425                 430         


Ser Arg Phe Arg Met Met Asn Leu Gln Gly Glu Glu Phe Val Cys Leu 
        435                 440                 445             


Lys Ser Ile Ile Leu Leu Asn Ser Gly Val Tyr Thr Phe Leu Ser Ser 
    450                 455                 460                 


Thr Leu Lys Ser Leu Glu Glu Lys Asp His Ile His Arg Val Leu Asp 
465                 470                 475                 480 


Lys Ile Thr Asp Thr Leu Ile His Leu Met Ala Lys Ala Gly Leu Thr 
                485                 490                 495     


Leu Gln Gln Gln His Gln Arg Leu Ala Gln Leu Leu Leu Ile Leu Ser 
            500                 505                 510         


His Ile Arg His Met Ser Asn Lys Arg Met Glu His Leu Tyr Ser Met 
        515                 520                 525             


Lys Cys Lys Asn Val Val Pro Leu Tyr Asp Leu Leu Leu Glu Met Leu 
    530                 535                 540                 


Asp Ala His Arg Leu His Ala Pro Thr Ser Arg Gly Gly Ala Ser Val 
545                 550                 555                 560 


Glu Glu Thr Asp Gln Ser His Leu Ala Thr Ala Gly Ser Thr Ser Ser 
                565                 570                 575     


His Ser Leu Gln Lys Tyr Tyr Ile Thr Gly Glu Ala Glu Gly Phe Pro 
            580                 585                 590         


Ala Thr Val 
        595 


<210>  11
<211>  314
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EBD (L384M, M421G, G521R) Pairs with estrogen analog CMP8

<400>  11

Ser Ala Gly Asp Met Arg Ala Ala Asn Leu Trp Pro Ser Pro Leu Met 
1               5                   10                  15      


Ile Lys Arg Ser Lys Lys Asn Ser Leu Ala Leu Ser Leu Thr Ala Asp 
            20                  25                  30          


Gln Met Val Ser Ala Leu Leu Asp Ala Glu Pro Pro Ile Leu Tyr Ser 
        35                  40                  45              


Glu Tyr Asp Pro Thr Arg Pro Phe Ser Glu Ala Ser Met Met Gly Leu 
    50                  55                  60                  


Leu Thr Asn Leu Ala Asp Arg Glu Leu Val His Met Ile Asn Trp Ala 
65                  70                  75                  80  


Lys Arg Val Pro Gly Phe Val Asp Leu Thr Leu His Asp Gln Val His 
                85                  90                  95      


Leu Leu Glu Cys Ala Trp Met Glu Ile Leu Met Ile Gly Leu Val Trp 
            100                 105                 110         


Arg Ser Met Glu His Pro Gly Lys Leu Leu Phe Ala Pro Asn Leu Leu 
        115                 120                 125             


Leu Asp Arg Asn Gln Gly Lys Cys Val Glu Gly Gly Val Glu Ile Phe 
    130                 135                 140                 


Asp Met Leu Leu Ala Thr Ser Ser Arg Phe Arg Met Met Asn Leu Gln 
145                 150                 155                 160 


Gly Glu Glu Phe Val Cys Leu Lys Ser Ile Ile Leu Leu Asn Ser Gly 
                165                 170                 175     


Val Tyr Thr Phe Leu Ser Ser Thr Leu Lys Ser Leu Glu Glu Lys Asp 
            180                 185                 190         


His Ile His Arg Val Leu Asp Lys Ile Thr Asp Thr Leu Ile His Leu 
        195                 200                 205             


Met Ala Lys Ala Gly Leu Thr Leu Gln Gln Gln His Gln Arg Leu Ala 
    210                 215                 220                 


Gln Leu Leu Leu Ile Leu Ser His Ile Arg His Met Ser Asn Lys Arg 
225                 230                 235                 240 


Met Glu His Leu Tyr Ser Met Lys Cys Lys Asn Val Val Pro Leu Tyr 
                245                 250                 255     


Asp Leu Leu Leu Glu Met Leu Asp Ala His Arg Leu His Ala Pro Thr 
            260                 265                 270         


Ser Arg Gly Gly Ala Ser Val Glu Glu Thr Asp Gln Ser His Leu Ala 
        275                 280                 285             


Thr Ala Gly Ser Thr Ser Ser His Ser Leu Gln Lys Tyr Tyr Ile Thr 
    290                 295                 300                 


Gly Glu Ala Glu Gly Phe Pro Ala Thr Val 
305                 310                 


<210>  12
<211>  595
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Estrogen Receptor with G400V, M543A, and L544A mutations

<400>  12

Met Thr Met Thr Leu His Thr Lys Ala Ser Gly Met Ala Leu Leu His 
1               5                   10                  15      


Gln Ile Gln Gly Asn Glu Leu Glu Pro Leu Asn Arg Pro Gln Leu Lys 
            20                  25                  30          


Ile Pro Leu Glu Arg Pro Leu Gly Glu Val Tyr Leu Asp Ser Ser Lys 
        35                  40                  45              


Pro Ala Val Tyr Asn Tyr Pro Glu Gly Ala Ala Tyr Glu Phe Asn Ala 
    50                  55                  60                  


Ala Ala Ala Ala Asn Ala Gln Val Tyr Gly Gln Thr Gly Leu Pro Tyr 
65                  70                  75                  80  


Gly Pro Gly Ser Glu Ala Ala Ala Phe Gly Ser Asn Gly Leu Gly Gly 
                85                  90                  95      


Phe Pro Pro Leu Asn Ser Val Ser Pro Ser Pro Leu Met Leu Leu His 
            100                 105                 110         


Pro Pro Pro Gln Leu Ser Pro Phe Leu Gln Pro His Gly Gln Gln Val 
        115                 120                 125             


Pro Tyr Tyr Leu Glu Asn Glu Pro Ser Gly Tyr Thr Val Arg Glu Ala 
    130                 135                 140                 


Gly Pro Pro Ala Phe Tyr Arg Pro Asn Ser Asp Asn Arg Arg Gln Gly 
145                 150                 155                 160 


Gly Arg Glu Arg Leu Ala Ser Thr Asn Asp Lys Gly Ser Met Ala Met 
                165                 170                 175     


Glu Ser Ala Lys Glu Thr Arg Tyr Cys Ala Val Cys Asn Asp Tyr Ala 
            180                 185                 190         


Ser Gly Tyr His Tyr Gly Val Trp Ser Cys Glu Gly Cys Lys Ala Phe 
        195                 200                 205             


Phe Lys Arg Ser Ile Gln Gly His Asn Asp Tyr Met Cys Pro Ala Thr 
    210                 215                 220                 


Asn Gln Cys Thr Ile Asp Lys Asn Arg Arg Lys Ser Cys Gln Ala Cys 
225                 230                 235                 240 


Arg Leu Arg Lys Cys Tyr Glu Val Gly Met Met Lys Gly Gly Ile Arg 
                245                 250                 255     


Lys Asp Arg Arg Gly Gly Arg Met Leu Lys His Lys Arg Gln Arg Asp 
            260                 265                 270         


Asp Gly Glu Gly Arg Gly Glu Val Gly Ser Ala Gly Asp Met Arg Ala 
        275                 280                 285             


Ala Asn Leu Trp Pro Ser Pro Leu Met Ile Lys Arg Ser Lys Lys Asn 
    290                 295                 300                 


Ser Leu Ala Leu Ser Leu Thr Ala Asp Gln Met Val Ser Ala Leu Leu 
305                 310                 315                 320 


Asp Ala Glu Pro Pro Ile Leu Tyr Ser Glu Tyr Asp Pro Thr Arg Pro 
                325                 330                 335     


Phe Ser Glu Ala Ser Met Met Gly Leu Leu Thr Asn Leu Ala Asp Arg 
            340                 345                 350         


Glu Leu Val His Met Ile Asn Trp Ala Lys Arg Val Pro Gly Phe Val 
        355                 360                 365             


Asp Leu Thr Leu His Asp Gln Val His Leu Leu Glu Cys Ala Trp Leu 
    370                 375                 380                 


Glu Ile Leu Met Ile Gly Leu Val Trp Arg Ser Met Glu His Pro Val 
385                 390                 395                 400 


Lys Leu Leu Phe Ala Pro Asn Leu Leu Leu Asp Arg Asn Gln Gly Lys 
                405                 410                 415     


Cys Val Glu Gly Met Val Glu Ile Phe Asp Met Leu Leu Ala Thr Ser 
            420                 425                 430         


Ser Arg Phe Arg Met Met Asn Leu Gln Gly Glu Glu Phe Val Cys Leu 
        435                 440                 445             


Lys Ser Ile Ile Leu Leu Asn Ser Gly Val Tyr Thr Phe Leu Ser Ser 
    450                 455                 460                 


Thr Leu Lys Ser Leu Glu Glu Lys Asp His Ile His Arg Val Leu Asp 
465                 470                 475                 480 


Lys Ile Thr Asp Thr Leu Ile His Leu Met Ala Lys Ala Gly Leu Thr 
                485                 490                 495     


Leu Gln Gln Gln His Gln Arg Leu Ala Gln Leu Leu Leu Ile Leu Ser 
            500                 505                 510         


His Ile Arg His Met Ser Asn Lys Gly Met Glu His Leu Tyr Ser Met 
        515                 520                 525             


Lys Cys Lys Asn Val Val Pro Leu Tyr Asp Leu Leu Leu Glu Ala Ala 
    530                 535                 540                 


Asp Ala His Arg Leu His Ala Pro Thr Ser Arg Gly Gly Ala Ser Val 
545                 550                 555                 560 


Glu Glu Thr Asp Gln Ser His Leu Ala Thr Ala Gly Ser Thr Ser Ser 
                565                 570                 575     


His Ser Leu Gln Lys Tyr Tyr Ile Thr Gly Glu Ala Glu Gly Phe Pro 
            580                 585                 590         


Ala Thr Val 
        595 


<210>  13
<211>  314
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EBD with G400V, M543A, and L544A mutations (ER(T2))

<400>  13

Ser Ala Gly Asp Met Arg Ala Ala Asn Leu Trp Pro Ser Pro Leu Met 
1               5                   10                  15      


Ile Lys Arg Ser Lys Lys Asn Ser Leu Ala Leu Ser Leu Thr Ala Asp 
            20                  25                  30          


Gln Met Val Ser Ala Leu Leu Asp Ala Glu Pro Pro Ile Leu Tyr Ser 
        35                  40                  45              


Glu Tyr Asp Pro Thr Arg Pro Phe Ser Glu Ala Ser Met Met Gly Leu 
    50                  55                  60                  


Leu Thr Asn Leu Ala Asp Arg Glu Leu Val His Met Ile Asn Trp Ala 
65                  70                  75                  80  


Lys Arg Val Pro Gly Phe Val Asp Leu Thr Leu His Asp Gln Val His 
                85                  90                  95      


Leu Leu Glu Cys Ala Trp Leu Glu Ile Leu Met Ile Gly Leu Val Trp 
            100                 105                 110         


Arg Ser Met Glu His Pro Val Lys Leu Leu Phe Ala Pro Asn Leu Leu 
        115                 120                 125             


Leu Asp Arg Asn Gln Gly Lys Cys Val Glu Gly Met Val Glu Ile Phe 
    130                 135                 140                 


Asp Met Leu Leu Ala Thr Ser Ser Arg Phe Arg Met Met Asn Leu Gln 
145                 150                 155                 160 


Gly Glu Glu Phe Val Cys Leu Lys Ser Ile Ile Leu Leu Asn Ser Gly 
                165                 170                 175     


Val Tyr Thr Phe Leu Ser Ser Thr Leu Lys Ser Leu Glu Glu Lys Asp 
            180                 185                 190         


His Ile His Arg Val Leu Asp Lys Ile Thr Asp Thr Leu Ile His Leu 
        195                 200                 205             


Met Ala Lys Ala Gly Leu Thr Leu Gln Gln Gln His Gln Arg Leu Ala 
    210                 215                 220                 


Gln Leu Leu Leu Ile Leu Ser His Ile Arg His Met Ser Asn Lys Gly 
225                 230                 235                 240 


Met Glu His Leu Tyr Ser Met Lys Cys Lys Asn Val Val Pro Leu Tyr 
                245                 250                 255     


Asp Leu Leu Leu Glu Ala Ala Asp Ala His Arg Leu His Ala Pro Thr 
            260                 265                 270         


Ser Arg Gly Gly Ala Ser Val Glu Glu Thr Asp Gln Ser His Leu Ala 
        275                 280                 285             


Thr Ala Gly Ser Thr Ser Ser His Ser Leu Gln Lys Tyr Tyr Ile Thr 
    290                 295                 300                 


Gly Glu Ala Glu Gly Phe Pro Ala Thr Val 
305                 310                 


<210>  14
<211>  942
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Alternative G400V, M543A, and L544A ER(T2) Coding Sequence

<400>  14
tcagcgggtg acatgcgagc tgccaatctc tggccgtcac ctttgatgat taaacgatca       60

aagaaaaata gcctggctct gagcttgacg gctgatcaaa tggtaagtgc actgctcgac      120

gccgaaccgc cgatcctgta ttccgagtat gaccccacac ggccattctc cgaagccagt      180

atgatgggac ttcttaccaa ccttgcagat cgcgaactcg tacatatgat aaattgggcg      240

aagcgcgttc cagggttcgt cgacctcacg ttgcatgatc aggtccatct gctggaatgc      300

gcgtggttgg aaatcttgat gattggtttg gtctggcgat ctatggagca tccggtgaaa      360

cttcttttcg cgcccaatct tcttttggat cgcaatcaag ggaaatgcgt ggaaggaatg      420

gtcgaaattt ttgatatgct tctcgcgacc agctcccgat tcaggatgat gaatttgcag      480

ggtgaagagt ttgtatgtct taagtctatt atattgctca actccggcgt ttatacattc      540

ctttctagca cacttaaatc actcgaggaa aaggaccaca tccaccgggt tttggataaa      600

atcactgata cactgattca tttgatggcc aaggccggac tcacactcca acaacagcac      660

caacggcttg cacagctcct cctcatactt tctcatataa ggcacatgtc aaataaggga      720

atggagcacc tgtactccat gaagtgtaaa aatgtagtcc ctctgtatga tctgctcctt      780

gaggctgcag acgcgcatag actgcacgcc ccaacgtcac gagggggggc ctcagtggaa      840

gagacagatc agagccatct cgcaaccgca ggatctacat ccagccactc tcttcagaaa      900

tactatataa ctggcgaagc cgaagggttc cctgccaccg tt                         942


<210>  15
<211>  942
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EBD (L384M, M421G, G521R) Coding Sequence

<400>  15
tctgctggcg atatgagagc cgctaacctg tggccttctc cactgatgat caagcggagc       60

aagaagaact ctctggccct gtctctgacc gccgaccaga tggtttctgc tctgctggat      120

gccgagcctc ctatcctgta ctccgagtac gaccccacca gaccttttag cgaggccagc      180

atgatgggcc tgctgaccaa tctggccgac agagaactgg tgcacatgat caactgggcc      240

aagcgcgtgc ccggctttgt cgatctgaca ctgcacgacc aagtgcatct gctggaatgc      300

gcctggatgg aaatcctgat gatcggcctc gtgtggcgga gcatggaaca ccctggaaaa      360

ctgctgttcg cccctaacct gctgctcgac agaaaccagg gcaaatgcgt ggaaggcggc      420

gtggaaatct tcgatatgct gctggccacc tccagccggt tccggatgat gaatctccag      480

ggcgaagagt tcgtgtgcct gaagtccatc atcctgctga atagcggcgt gtacacattc      540

ctgagcagca ccctgaaatc cctggaagag aaggaccaca tccaccgggt gctcgacaag      600

atcaccgaca cactgatcca cctgatggcc aaggccggac tgactctgca gcagcaacat      660

cagagactgg cccagctgct gctcatcctg agccacatca gacacatgag caacaagcgg      720

atggaacatc tgtactccat gaagtgcaag aacgtggtgc ccctgtacga cctgctcctg      780

gaaatgctgg acgcccacag actgcacgcc cctacatcta gaggtggcgc ctctgtggaa      840

gagacagacc agtctcacct ggccacagcc ggcagcacaa gctctcattc cctgcagaag      900

tactacatca ccggcgaggc cgagggcttt ccagccacag tt                         942


<210>  16
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Epidermal growth factor receptor (EGFR) (806) single chain 
       variable (scFv) antibody fragment

<400>  16

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Asp Val Gln Leu Gln Glu Ser Gly Pro Ser 
            20                  25                  30          


Leu Val Lys Pro Ser Gln Ser Leu Ser Leu Thr Cys Thr Val Thr Gly 
        35                  40                  45              


Tyr Ser Ile Thr Ser Asp Phe Ala Trp Asn Trp Ile Arg Gln Phe Pro 
    50                  55                  60                  


Gly Asn Lys Leu Glu Trp Met Gly Tyr Ile Ser Tyr Ser Gly Asn Thr 
65                  70                  75                  80  


Arg Tyr Asn Pro Ser Leu Lys Ser Arg Ile Ser Ile Thr Arg Asp Thr 
                85                  90                  95      


Ser Lys Asn Gln Phe Phe Leu Gln Leu Asn Ser Val Thr Ile Glu Asp 
            100                 105                 110         


Thr Ala Thr Tyr Tyr Cys Val Thr Ala Gly Arg Gly Phe Pro Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Leu Val Thr Val Ser Ala Gly Ser Thr Ser Gly Ser 
    130                 135                 140                 


Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr Lys Gly Asp Ile Leu Met 
145                 150                 155                 160 


Thr Gln Ser Pro Ser Ser Met Ser Val Ser Leu Gly Asp Thr Val Ser 
                165                 170                 175     


Ile Thr Cys His Ser Ser Gln Asp Ile Asn Ser Asn Ile Gly Trp Leu 
            180                 185                 190         


Gln Gln Arg Pro Gly Lys Ser Phe Lys Gly Leu Ile Tyr His Gly Thr 
        195                 200                 205             


Asn Leu Asp Asp Glu Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly 
    210                 215                 220                 


Ala Asp Tyr Ser Leu Thr Ile Ser Ser Leu Glu Ser Glu Asp Phe Ala 
225                 230                 235                 240 


Asp Tyr Tyr Cys Val Gln Tyr Ala Gln Phe Pro Trp Thr Phe Gly Gly 
                245                 250                 255     


Gly Thr Lys Leu Glu Ile Lys Arg 
            260                 


<210>  17
<211>  787
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EGFRVIII (806) scFv Coding Sequence

<400>  17
atgctgctgc tggtgaccag cctgctgctg tgcgagctgc cccaccccgc ctttctgctg       60

atcccccagg tgcagctgca gcagcctggc gccgagctgg tgaagccagg cgccagcgtg      120

aagctgtcct gcaaggccag cggctacacc ttcaccggct actggatgca ctgggtgaag      180

cagagacccg gccacggcct ggaatggatc ggcgagatca accccagcaa cggccggacc      240

aactacaacg agcggttcaa gagcaaggcc accctgaccg tggacaagag cagcaccacc      300

gccttcatgc agctgtccgg cctgaccagc gaggacagcg ccgtgtactt ctgcgccagg      360

gactactacg gcaccagcta caacttcgac tactggggcc agggcaccac actgaccgtg      420

agcagcggcg gagggggctc tggcggcgga ggatctgggg gagggggcag cgacatccag      480

atgacccaga gcagcagcag cttcagcgtg agcctgggcg accgggtgac catcacctgt      540

aaggccaacg aggacatcaa caaccggctg gcctggtatc agcagacccc cggcaacagc      600

cccaggctgc tgatcagcgg cgccaccaac ctggtgaccg gcgtgcccag ccggtttagc      660

ggcagcggct ccggcaagga ctacaccctg accatcacaa gcctgcaggc cgaggacttc      720

gccacctact actgccagca gtactggtcc acccccttca ccttcggcag cggcaccgag      780

ctggaaa                                                                787


<210>  18
<211>  265
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFR scFv

<400>  18

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gln Val Gln Leu Lys Gln Ser Gly Pro Gly 
            20                  25                  30          


Leu Val Gln Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser Gly 
        35                  40                  45              


Phe Ser Leu Thr Asn Tyr Gly Val His Trp Val Arg Gln Ser Pro Gly 
    50                  55                  60                  


Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Ser Gly Gly Asn Thr Asp 
65                  70                  75                  80  


Tyr Asn Thr Pro Phe Thr Ser Arg Leu Ser Ile Asn Lys Asp Asn Ser 
                85                  90                  95      


Lys Ser Gln Val Phe Phe Lys Met Asn Ser Leu Gln Ser Asn Asp Thr 
            100                 105                 110         


Ala Ile Tyr Tyr Cys Ala Arg Ala Leu Thr Tyr Tyr Asp Tyr Glu Phe 
        115                 120                 125             


Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala Gly Gly Gly 
    130                 135                 140                 


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Leu Leu 
145                 150                 155                 160 


Thr Gln Ser Pro Val Ile Leu Ser Val Ser Pro Gly Glu Arg Val Ser 
                165                 170                 175     


Phe Ser Cys Arg Ala Ser Gln Ser Ile Gly Thr Asn Ile His Trp Tyr 
            180                 185                 190         


Gln Gln Arg Thr Asn Gly Ser Pro Arg Leu Leu Ile Lys Tyr Ala Ser 
        195                 200                 205             


Glu Ser Ile Ser Gly Ile Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly 
    210                 215                 220                 


Thr Asp Phe Thr Leu Ser Ile Asn Ser Val Glu Ser Glu Asp Ile Ala 
225                 230                 235                 240 


Asp Tyr Tyr Cys Gln Gln Asn Asn Asn Trp Pro Thr Thr Phe Gly Ala 
                245                 250                 255     


Gly Thr Lys Leu Glu Leu Lys Arg Thr 
            260                 265 


<210>  19
<211>  268
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD19 (huCD19) (G01S) scFv

<400>  19

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
            20                  25                  30          


Leu Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              


Phe Thr Phe Asp Asp Tyr Ala Met His Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Lys Gly Leu Glu Trp Val Ser Gly Ile Ser Trp Asn Ser Gly Arg Ile 
65                  70                  75                  80  


Gly Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
                85                  90                  95      


Ala Lys Asn Ser Leu Phe Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Asp Gln Gly Tyr His Tyr Tyr Asp 
        115                 120                 125             


Ser Ala Glu His Ala Phe Asp Ile Trp Gly Gln Gly Thr Val Val Thr 
    130                 135                 140                 


Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
145                 150                 155                 160 


Gly Ser Gln Ser Ala Leu Thr Gln Pro Arg Ser Val Ser Gly Phe Pro 
                165                 170                 175     


Gly Gln Ser Val Thr Ile Ser Cys Thr Gly Thr Thr Ser Asp Asp Val 
            180                 185                 190         


Ser Trp Tyr Gln Gln His Pro Gly Lys Ala Pro Gln Leu Met Leu Tyr 
        195                 200                 205             


Asp Val Ser Lys Arg Pro Ser Gly Val Pro His Arg Phe Ser Gly Ser 
    210                 215                 220                 


Arg Ser Gly Arg Ala Ala Ser Leu Ile Ile Ser Gly Leu Gln Thr Glu 
225                 230                 235                 240 


Asp Glu Ala Asp Tyr Phe Cys Ser Ser Tyr Ala Gly Arg Tyr Asn Ser 
                245                 250                 255     


Val Leu Phe Gly Gly Gly Thr Lys Leu Thr Val Leu 
            260                 265             


<210>  20
<211>  267
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse CD19 (muCD19) (FMC63) scFv

<400>  20

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser 
            20                  25                  30          


Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser 
        35                  40                  45              


Gln Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly 
    50                  55                  60                  


Thr Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val 
65                  70                  75                  80  


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr 
                85                  90                  95      


Ile Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln 
            100                 105                 110         


Gly Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
        115                 120                 125             


Thr Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser 
    130                 135                 140                 


Thr Lys Gly Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala 
145                 150                 155                 160 


Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu 
                165                 170                 175     


Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu 
            180                 185                 190         


Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser 
        195                 200                 205             


Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln 
    210                 215                 220                 


Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr 
225                 230                 235                 240 


Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr 
                245                 250                 255     


Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 
            260                 265         


<210>  21
<211>  244
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD19 scFv

<400>  21

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Leu Gly Asp 
1               5                   10                  15      


Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr Leu 
            20                  25                  30          


Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile Tyr 
        35                  40                  45              


His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln Glu 
65                  70                  75                  80  


Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr Thr 
                85                  90                  95      


Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Ser Thr Ser Gly Ser 
            100                 105                 110         


Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr Lys Gly Glu Val Lys Leu 
        115                 120                 125             


Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Val 
    130                 135                 140                 


Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser Trp 
145                 150                 155                 160 


Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile Trp 
                165                 170                 175     


Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr 
            180                 185                 190         


Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser 
        195                 200                 205             


Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr 
    210                 215                 220                 


Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val 
225                 230                 235                 240 


Thr Val Ser Ser 
                


<210>  22
<211>  735
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD19 scFv Coding Sequence

<400>  22
gacatccaga tgacccagac cacctccagc ctgagcgcca gcctgggcga ccgggtgacc       60

atcagctgcc gggccagcca ggacatcagc aagtacctga actggtatca gcagaagccc      120

gacggcaccg tcaagctgct gatctaccac accagccggc tgcacagcgg cgtgcccagc      180

cggtttagcg gcagcggctc cggcaccgac tacagcctga ccatctccaa cctggaacag      240

gaagatatcg ccacctactt ttgccagcag ggcaacacac tgccctacac ctttggcggc      300

ggaacaaagc tggaaatcac cggcagcacc tccggcagcg gcaagcctgg cagcggcgag      360

ggcagcacca agggcgaggt gaagctgcag gaaagcggcc ctggcctggt ggcccccagc      420

cagagcctga gcgtgacctg caccgtgagc ggcgtgagcc tgcccgacta cggcgtgagc      480

tggatccggc agccccccag gaagggcctg gaatggctgg gcgtgatctg gggcagcgag      540

accacctact acaacagcgc cctgaagagc cggctgacca tcatcaagga caacagcaag      600

agccaggtgt tcctgaagat gaacagcctg cagaccgacg acaccgccat ctactactgc      660

gccaagcact actactacgg cggcagctac gccatggact actggggcca gggcaccagc      720

gtgaccgtga gcagc                                                       735


<210>  23
<211>  266
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD20 (Leu 16) scFv

<400>  23

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Ile Val Leu Thr Gln Ser Pro Ala Ile Leu Ser 
            20                  25                  30          


Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser Ser Ser 
        35                  40                  45              


Val Asn Tyr Met Asp Trp Tyr Gln Lys Lys Pro Gly Ser Ser Pro Lys 
    50                  55                  60                  


Pro Trp Ile Tyr Ala Thr Ser Asn Leu Ala Ser Gly Val Pro Ala Arg 
65                  70                  75                  80  


Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg 
                85                  90                  95      


Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Phe 
            100                 105                 110         


Asn Pro Pro Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Gly Ser 
        115                 120                 125             


Thr Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser 
    130                 135                 140                 


Glu Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Lys Pro Gly Ala 
145                 150                 155                 160 


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
                165                 170                 175     


Asn Met His Trp Val Lys Gln Thr Pro Gly Gln Gly Leu Glu Trp Ile 
            180                 185                 190         


Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 
        195                 200                 205             


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
    210                 215                 220                 


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Asp Tyr Tyr Cys 
225                 230                 235                 240 


Ala Arg Ser Asn Tyr Tyr Gly Ser Ser Tyr Trp Phe Phe Asp Val Trp 
                245                 250                 255     


Gly Ala Gly Thr Thr Val Thr Val Ser Ser 
            260                 265     


<210>  24
<211>  798
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD20 scFv Coding Sequence

<400>  24
atggagacag acacactcct gctatgggtg ctgctgctct gggttccagg ttccacaggt       60

gacattgtgc tgacccaatc tccagctatc ctgtctgcat ctccagggga gaaggtcaca      120

atgacttgca gggccagctc aagtgtaaat tacatggact ggtaccagaa gaagccagga      180

tcctccccca aaccctggat ttatgccaca tccaacctgg cttctggagt ccctgctcgc      240

ttcagtggca gtgggtctgg gacctcttac tctctcacaa tcagcagagt ggaggctgaa      300

gatgctgcca cttattactg ccagcagtgg agttttaatc cacccacgtt cggagggggg      360

accaagctgg aaataaaagg cagtactagc ggtggtggct ccgggggcgg ttccggtggg      420

ggcggcagca gcgaggtgca gctgcagcag tctggggctg agctggtgaa gcctggggcc      480

tcagtgaaga tgtcctgcaa ggcttctggc tacacattta ccagttacaa tatgcactgg      540

gtaaagcaga cacctggaca gggcctggaa tggattggag ctatttatcc aggaaatggt      600

gatacttcct acaatcagaa gttcaaaggc aaggccacat tgactgcaga caaatcctcc      660

agcacagcct acatgcagct cagcagcctg acatctgagg actctgcgga ctattactgt      720

gcaagatcta attattacgg tagtagctac tggttcttcg atgtctgggg cgcagggacc      780

acggtcaccg tctcctca                                                    798


<210>  25
<211>  270
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD22 (m971) scFv

<400>  25

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gln Val Gln Leu Gln Gln Ser Gly Pro Gly 
            20                  25                  30          


Leu Val Lys Pro Ser Gln Thr Leu Ser Leu Thr Cys Ala Ile Ser Gly 
        35                  40                  45              


Asp Ser Val Ser Ser Asn Ser Ala Ala Trp Asn Trp Ile Arg Gln Ser 
    50                  55                  60                  


Pro Ser Arg Gly Leu Glu Trp Leu Gly Arg Thr Tyr Tyr Arg Ser Lys 
65                  70                  75                  80  


Trp Tyr Asn Asp Tyr Ala Val Ser Val Lys Ser Arg Ile Thr Ile Asn 
                85                  90                  95      


Pro Asp Thr Ser Lys Asn Gln Phe Ser Leu Gln Leu Asn Ser Val Thr 
            100                 105                 110         


Pro Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Glu Val Thr Gly Asp 
        115                 120                 125             


Leu Glu Asp Ala Phe Asp Ile Trp Gly Gln Gly Thr Met Val Thr Val 
    130                 135                 140                 


Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
145                 150                 155                 160 


Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val 
                165                 170                 175     


Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Thr Ile Trp Ser 
            180                 185                 190         


Tyr Leu Asn Trp Tyr Gln Gln Arg Pro Gly Lys Ala Pro Asn Leu Leu 
        195                 200                 205             


Ile Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser 
    210                 215                 220                 


Gly Arg Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln 
225                 230                 235                 240 


Ala Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Tyr Ser Ile Pro 
                245                 250                 255     


Gln Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Thr 
            260                 265                 270 


<210>  26
<211>  316
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B7H3 (hBRCA84D) scFv

<400>  26

Met Leu Arg Arg Arg Gly Ser Pro Gly Met Gly Val His Val Gly Ala 
1               5                   10                  15      


Ala Leu Gly Ala Leu Trp Phe Cys Leu Thr Gly Ala Leu Glu Val Gln 
            20                  25                  30          


Val Pro Glu Asp Pro Val Val Ala Leu Val Gly Thr Asp Ala Thr Leu 
        35                  40                  45              


Cys Cys Ser Phe Ser Pro Glu Pro Gly Phe Ser Leu Ala Gln Leu Asn 
    50                  55                  60                  


Leu Ile Trp Gln Leu Thr Asp Thr Lys Gln Leu Val His Ser Phe Ala 
65                  70                  75                  80  


Glu Gly Gln Asp Gln Gly Ser Ala Tyr Ala Asn Arg Thr Ala Leu Phe 
                85                  90                  95      


Pro Asp Leu Leu Ala Gln Gly Asn Ala Ser Leu Arg Leu Gln Arg Val 
            100                 105                 110         


Arg Val Ala Asp Glu Gly Ser Phe Thr Cys Phe Val Ser Ile Arg Asp 
        115                 120                 125             


Phe Gly Ser Ala Ala Val Ser Leu Gln Val Ala Ala Pro Tyr Ser Lys 
    130                 135                 140                 


Pro Ser Met Thr Leu Glu Pro Asn Lys Asp Leu Arg Pro Gly Asp Thr 
145                 150                 155                 160 


Val Thr Ile Thr Cys Ser Ser Tyr Gln Gly Tyr Pro Glu Ala Glu Val 
                165                 170                 175     


Phe Trp Gln Asp Gly Gln Gly Val Pro Leu Thr Gly Asn Val Thr Thr 
            180                 185                 190         


Ser Gln Met Ala Asn Glu Gln Gly Leu Phe Asp Val His Ser Ile Leu 
        195                 200                 205             


Arg Val Val Leu Gly Ala Asn Gly Thr Tyr Ser Cys Leu Val Arg Asn 
    210                 215                 220                 


Pro Val Leu Gln Gln Asp Ala His Ser Ser Val Thr Ile Thr Gly Gln 
225                 230                 235                 240 


Pro Met Thr Phe Pro Pro Glu Ala Leu Trp Val Thr Val Gly Leu Ser 
                245                 250                 255     


Val Cys Leu Ile Ala Leu Leu Val Ala Leu Ala Phe Val Cys Trp Arg 
            260                 265                 270         


Lys Ile Lys Gln Ser Cys Glu Glu Glu Asn Ala Gly Ala Glu Asp Gln 
        275                 280                 285             


Asp Gly Glu Gly Glu Gly Ser Lys Thr Ala Leu Gln Pro Leu Lys His 
    290                 295                 300                 


Ser Asp Ser Lys Glu Asp Asp Gly Gln Glu Ile Ala 
305                 310                 315     


<210>  27
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  L1CAM (CE7) scFv

<400>  27

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gln Val Gln Leu Gln Gln Pro Gly Ala Glu 
            20                  25                  30          


Leu Val Lys Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly 
        35                  40                  45              


Tyr Thr Phe Thr Gly Tyr Trp Met His Trp Val Lys Gln Arg Pro Gly 
    50                  55                  60                  


His Gly Leu Glu Trp Ile Gly Glu Ile Asn Pro Ser Asn Gly Arg Thr 
65                  70                  75                  80  


Asn Tyr Asn Glu Arg Phe Lys Ser Lys Ala Thr Leu Thr Val Asp Lys 
                85                  90                  95      


Ser Ser Thr Thr Ala Phe Met Gln Leu Ser Gly Leu Thr Ser Glu Asp 
            100                 105                 110         


Ser Ala Val Tyr Phe Cys Ala Arg Asp Tyr Tyr Gly Thr Ser Tyr Asn 
        115                 120                 125             


Phe Asp Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln 
145                 150                 155                 160 


Met Thr Gln Ser Ser Ser Ser Phe Ser Val Ser Leu Gly Asp Arg Val 
                165                 170                 175     


Thr Ile Thr Cys Lys Ala Asn Glu Asp Ile Asn Asn Arg Leu Ala Trp 
            180                 185                 190         


Tyr Gln Gln Thr Pro Gly Asn Ser Pro Arg Leu Leu Ile Ser Gly Ala 
        195                 200                 205             


Thr Asn Leu Val Thr Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser 
    210                 215                 220                 


Gly Lys Asp Tyr Thr Leu Thr Ile Thr Ser Leu Gln Ala Glu Asp Phe 
225                 230                 235                 240 


Ala Thr Tyr Tyr Cys Gln Gln Tyr Trp Ser Thr Pro Phe Thr Phe Gly 
                245                 250                 255     


Ser Gly Thr Glu Leu Glu Ile Lys 
            260                 


<210>  28
<211>  259
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EphA2 (2A4) scFv

<400>  28

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gln Val Gln Leu Leu Glu Ser Gly Gly Gly 
            20                  25                  30          


Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              


Phe Thr Phe Ser Ser Tyr Thr Met Ser Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Gln Ala Leu Glu Trp Met Gly Thr Ile Ser Ser Arg Gly Thr Tyr Thr 
65                  70                  75                  80  


Tyr Tyr Pro Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
                85                  90                  95      


Ala Lys Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Glu Ala Ile Phe Thr His Trp Gly 
        115                 120                 125             


Arg Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Leu Thr Gln Ser Pro 
145                 150                 155                 160 


Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Lys 
                165                 170                 175     


Ala Ser Gln Asp Ile Asn Asn Tyr His Ser Trp Tyr Gln Gln Lys Pro 
            180                 185                 190         


Gly Gln Ala Pro Arg Leu Leu Ile Tyr Arg Ala Asn Arg Leu Val Asp 
        195                 200                 205             


Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Tyr Gly Thr Asp Phe Thr 
    210                 215                 220                 


Leu Thr Ile Asn Asn Ile Glu Ser Glu Asp Ala Ala Tyr Tyr Phe Cys 
225                 230                 235                 240 


Leu Lys Tyr Asn Val Phe Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val 
                245                 250                 255     


Glu Ile Lys 
            


<210>  29
<211>  259
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EpHA2 (4H5) scFv

<400>  29

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gln Val Gln Leu Leu Glu Ser Gly Gly Gly 
            20                  25                  30          


Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              


Phe Thr Phe Ser Ser Tyr Thr Met Ser Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Gln Ala Leu Glu Trp Met Gly Thr Ile Ser Ser Gly Gly Thr Tyr Thr 
65                  70                  75                  80  


Tyr Tyr Pro Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
                85                  90                  95      


Ala Lys Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Glu Ala Ile Phe Thr Tyr Trp Gly 
        115                 120                 125             


Arg Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Leu Thr Gln Ser Pro 
145                 150                 155                 160 


Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Lys 
                165                 170                 175     


Ala Ser Gln Asp Ile Asn Asn Tyr Leu Ser Trp Tyr Gln Gln Lys Pro 
            180                 185                 190         


Gly Gln Ala Pro Arg Leu Leu Ile Tyr Arg Ala Asn Arg Leu Val Asp 
        195                 200                 205             


Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Tyr Gly Thr Asp Phe Thr 
    210                 215                 220                 


Leu Thr Ile Asn Asn Ile Glu Ser Glu Asp Ala Ala Tyr Tyr Phe Cys 
225                 230                 235                 240 


Leu Lys Tyr Asp Val Phe Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val 
                245                 250                 255     


Glu Ile Lys 
            


<210>  30
<211>  273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FITC (E2) scFv

<400>  30

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Ser Val Leu Thr Gln Pro Ser Ser Val Ser 
            20                  25                  30          


Ala Ala Pro Gly Gln Lys Val Thr Ile Ser Cys Ser Gly Ser Thr Ser 
        35                  40                  45              


Asn Ile Gly Asn Asn Tyr Val Ser Trp Tyr Gln Gln His Pro Gly Lys 
    50                  55                  60                  


Ala Pro Lys Leu Met Ile Tyr Asp Val Ser Lys Arg Pro Ser Gly Val 
65                  70                  75                  80  


Pro Asp Arg Phe Ser Gly Ser Lys Ser Gly Asn Ser Ala Ser Leu Asp 
                85                  90                  95      


Ile Ser Gly Leu Gln Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ala Ala 
            100                 105                 110         


Trp Asp Asp Ser Leu Ser Glu Phe Leu Phe Gly Thr Gly Thr Lys Leu 
        115                 120                 125             


Thr Val Leu Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gln Val Gln Leu Val Glu Ser Gly Gly Asn Leu Val Gln 
145                 150                 155                 160 


Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 
                165                 170                 175     


Gly Ser Phe Ser Met Ser Trp Val Arg Gln Ala Pro Gly Gly Gly Leu 
            180                 185                 190         


Glu Trp Val Ala Gly Leu Ser Ala Arg Ser Ser Leu Thr His Tyr Ala 
        195                 200                 205             


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn 
    210                 215                 220                 


Ser Val Tyr Leu Gln Met Asn Ser Leu Arg Val Glu Asp Thr Ala Val 
225                 230                 235                 240 


Tyr Tyr Cys Ala Arg Arg Ser Tyr Asp Ser Ser Gly Tyr Trp Gly His 
                245                 250                 255     


Phe Tyr Ser Tyr Met Asp Val Trp Gly Gln Gly Thr Leu Val Thr Val 
            260                 265                 270         


Ser 
    


<210>  31
<211>  127
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GD2 (hu3F8) scFv

<400>  31

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Ser Ile Val Met Thr Gln Thr Pro Lys Phe 
            20                  25                  30          


Leu Leu Val Ser Ala Gly Asp Arg Val Thr Ile Thr Cys Lys Ala Ser 
        35                  40                  45              


Gln Ser Val Ser Asn Asp Val Thr Trp Tyr Gln Gln Lys Ala Gly Gln 
    50                  55                  60                  


Ser Pro Lys Leu Leu Ile Tyr Ser Ala Ser Asn Arg Tyr Ser Gly Val 
65                  70                  75                  80  


Pro Asp Arg Phe Thr Gly Ser Gly Tyr Gly Thr Ala Phe Thr Phe Thr 
                85                  90                  95      


Ile Ser Thr Val Gln Ala Glu Asp Leu Ala Val Tyr Phe Cys Gln Gln 
            100                 105                 110         


Asp Tyr Ser Ser Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
        115                 120                 125         


<210>  32
<211>  267
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Her2 (Herceptin) scFv

<400>  32

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Asp Ile Gln Met Thr Gln Ser Pro Ser Ser 
            20                  25                  30          


Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser 
        35                  40                  45              


Gln Asp Val Asn Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys 
    50                  55                  60                  


Ala Pro Lys Leu Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val 
65                  70                  75                  80  


Pro Ser Arg Phe Ser Gly Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr 
                85                  90                  95      


Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln 
            100                 105                 110         


His Tyr Thr Thr Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile 
        115                 120                 125             


Lys Gly Ser Thr Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly 
    130                 135                 140                 


Gly Ser Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln 
145                 150                 155                 160 


Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile 
                165                 170                 175     


Lys Asp Thr Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
            180                 185                 190         


Glu Trp Val Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala 
        195                 200                 205             


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn 
    210                 215                 220                 


Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 
225                 230                 235                 240 


Tyr Tyr Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr 
                245                 250                 255     


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
            260                 265         


<210>  33
<211>  240
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL13Ra2 (hu08) VlVh scFv

<400>  33

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Asp Ile Gln Met Thr Gln Ser Pro Ser Ser 
            20                  25                  30          


Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Lys Ala Ser 
        35                  40                  45              


Gln Asp Val Gly Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys 
    50                  55                  60                  


Ala Pro Lys Leu Leu Ile Tyr Ser Ala Ser Tyr Arg Ser Thr Gly Val 
65                  70                  75                  80  


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
                85                  90                  95      


Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln His 
            100                 105                 110         


His Tyr Ser Ala Pro Trp Thr Phe Gly Gly Gly Thr Lys Val Glu Ile 
        115                 120                 125             


Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
145                 150                 155                 160 


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Arg Asn 
                165                 170                 175     


Gly Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
            180                 185                 190         


Ala Thr Val Ser Ser Gly Gly Ser Tyr Ile Tyr Tyr Ala Asp Ser Val 
        195                 200                 205             


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
    210                 215                 220                 


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
225                 230                 235                 240 


<210>  34
<211>  269
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL13Ra2 hu08 VhVl scFv

<400>  34

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
            20                  25                  30          


Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              


Phe Thr Phe Ser Arg Asn Gly Met Ser Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Lys Gly Leu Glu Trp Val Ala Thr Val Ser Ser Gly Gly Ser Tyr Ile 
65                  70                  75                  80  


Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
                85                  90                  95      


Ala Lys Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Gln Gly Thr Thr Ala Leu Ala Thr 
        115                 120                 125             


Arg Phe Phe Asp Val Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp 
145                 150                 155                 160 


Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp 
                165                 170                 175     


Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asp Val Gly Thr Ala Val 
            180                 185                 190         


Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr 
        195                 200                 205             


Ser Ala Ser Tyr Arg Ser Thr Gly Val Pro Ser Arg Phe Ser Gly Ser 
    210                 215                 220                 


Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu 
225                 230                 235                 240 


Asp Phe Ala Thr Tyr Tyr Cys Gln His His Tyr Ser Ala Pro Trp Thr 
                245                 250                 255     


Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Glu Ser Lys 
            260                 265                 


<210>  35
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL13Ra2 (hu07) VhVl scFv

<400>  35

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Asp Ile Gln Met Thr Gln Ser Pro Ser Ser 
            20                  25                  30          


Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Thr Ala Ser 
        35                  40                  45              


Leu Ser Val Ser Ser Thr Tyr Leu His Trp Tyr Gln Gln Lys Pro Gly 
    50                  55                  60                  


Lys Ala Pro Lys Leu Leu Ile Tyr Ser Thr Ser Asn Leu Ala Ser Gly 
65                  70                  75                  80  


Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
                85                  90                  95      


Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys His 
            100                 105                 110         


Gln Tyr His Arg Ser Pro Leu Thr Phe Gly Gly Gly Thr Lys Val Glu 
        115                 120                 125             


Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly 
145                 150                 155                 160 


Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Thr Lys 
                165                 170                 175     


Tyr Gly Val His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp 
            180                 185                 190         


Val Ala Val Lys Trp Ala Gly Gly Ser Thr Asp Tyr Asn Ser Ala Leu 
        195                 200                 205             


Met Ser Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
    210                 215                 220                 


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
225                 230                 235                 240 


Ala Arg Asp His Arg Asp Ala Met Asp Tyr Trp Gly Gln Gly Thr Leu 
                245                 250                 255     


Val Thr Val Ser Ser Glu Ser Lys 
            260                 


<210>  36
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL13Ra2 (hu07) VhVl scFv

<400>  36

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
            20                  25                  30          


Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              


Phe Thr Phe Thr Lys Tyr Gly Val His Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Lys Gly Leu Glu Trp Val Ala Val Lys Trp Ala Gly Gly Ser Thr Asp 
65                  70                  75                  80  


Tyr Asn Ser Ala Leu Met Ser Arg Phe Thr Ile Ser Arg Asp Asn Ala 
                85                  90                  95      


Lys Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr 
            100                 105                 110         


Ala Val Tyr Tyr Cys Ala Arg Asp His Arg Asp Ala Met Asp Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser 
145                 150                 155                 160 


Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys 
                165                 170                 175     


Thr Ala Ser Leu Ser Val Ser Ser Thr Tyr Leu His Trp Tyr Gln Gln 
            180                 185                 190         


Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ser Thr Ser Asn Leu 
        195                 200                 205             


Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
225                 230                 235                 240 


Tyr Cys His Gln Tyr His Arg Ser Pro Leu Thr Phe Gly Gly Gly Thr 
                245                 250                 255     


Lys Val Glu Ile Lys Glu Ser Lys 
            260                 


<210>  37
<211>  269
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  oaGD2 (8B6) VlVh scFv

<400>  37

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Asp Val Val Met Thr Gln Thr Pro Leu Ser 
            20                  25                  30          


Leu Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser 
        35                  40                  45              


Gln Ser Leu Leu Lys Asn Asn Gly Asn Thr Phe Leu His Trp Tyr Leu 
    50                  55                  60                  


Gln Lys Ser Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn 
65                  70                  75                  80  


Arg Leu Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr 
                85                  90                  95      


Tyr Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val 
            100                 105                 110         


Tyr Phe Cys Ser Gln Ser Thr His Ile Pro Tyr Thr Phe Gly Gly Gly 
        115                 120                 125             


Thr Lys Leu Glu Leu Lys Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Val Glu Ser Gly Gly Gly 
145                 150                 155                 160 


Leu Val Leu Pro Gly Asp Ser Leu Arg Leu Ser Cys Ala Thr Ser Glu 
                165                 170                 175     


Phe Thr Phe Thr Asp Tyr Tyr Met Thr Trp Val Arg Gln Pro Pro Arg 
            180                 185                 190         


Lys Ala Leu Glu Trp Leu Gly Phe Ile Arg Asn Arg Ala Asn Gly Tyr 
        195                 200                 205             


Thr Thr Glu Tyr Asn Pro Ser Val Lys Gly Arg Phe Thr Ile Ser Arg 
    210                 215                 220                 


Asp Asn Ser Gln Ser Ile Leu Tyr Leu Gln Met Asn Thr Leu Arg Thr 
225                 230                 235                 240 


Glu Asp Ser Ala Thr Tyr Tyr Cys Ala Arg Val Ser Asn Trp Ala Phe 
                245                 250                 255     


Asp Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser 
            260                 265                 


<210>  38
<211>  270
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ROR1 (R12) scFv

<400>  38

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gln Glu Gln Leu Val Glu Ser Gly Gly Arg 
            20                  25                  30          


Leu Val Thr Pro Gly Gly Ser Leu Thr Leu Ser Cys Lys Ala Ser Gly 
        35                  40                  45              


Phe Asp Phe Ser Ala Tyr Tyr Met Ser Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Lys Gly Leu Glu Trp Ile Ala Thr Ile Tyr Pro Ser Ser Gly Lys Thr 
65                  70                  75                  80  


Tyr Tyr Ala Thr Trp Val Asn Gly Arg Phe Thr Ile Ser Ser Asp Asn 
                85                  90                  95      


Ala Gln Asn Thr Val Asp Leu Gln Met Asn Ser Leu Thr Ala Ala Asp 
            100                 105                 110         


Arg Ala Thr Tyr Phe Cys Ala Arg Asp Ser Tyr Ala Asp Asp Gly Ala 
        115                 120                 125             


Leu Phe Asn Ile Trp Gly Pro Gly Thr Leu Val Thr Ile Ser Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Leu 
145                 150                 155                 160 


Val Leu Thr Gln Ser Pro Ser Val Ser Ala Ala Leu Gly Ser Pro Ala 
                165                 170                 175     


Lys Ile Thr Cys Thr Leu Ser Ser Ala His Lys Thr Asp Thr Ile Asp 
            180                 185                 190         


Trp Tyr Gln Gln Leu Gln Gly Glu Ala Pro Arg Tyr Leu Met Gln Val 
        195                 200                 205             


Gln Ser Asp Gly Ser Tyr Thr Lys Arg Pro Gly Val Pro Asp Arg Phe 
    210                 215                 220                 


Ser Gly Ser Ser Ser Gly Ala Asp Arg Tyr Leu Ile Ile Pro Ser Val 
225                 230                 235                 240 


Gln Ala Asp Asp Glu Ala Asp Tyr Tyr Cys Gly Ala Asp Tyr Ile Gly 
                245                 250                 255     


Gly Tyr Val Phe Gly Gly Gly Thr Gln Leu Thr Val Thr Gly 
            260                 265                 270 


<210>  39
<211>  139
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD33 (h2H12) VhVl scFv

<400>  39

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gln Val Gln Leu Val Gln Ser Gly Ala Glu 
            20                  25                  30          


Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly 
        35                  40                  45              


Tyr Thr Phe Thr Asn Tyr Asp Ile Asn Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Gln Gly Leu Glu Trp Ile Gly Trp Ile Tyr Pro Gly Asp Gly Ser Thr 
65                  70                  75                  80  


Lys Tyr Asn Glu Lys Phe Lys Ala Lys Ala Thr Leu Thr Ala Asp Thr 
                85                  90                  95      


Ser Thr Ser Thr Ala Tyr Met Glu Leu Arg Ser Leu Arg Ser Asp Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Ser Gly Tyr Glu Asp Ala Met Asp Tyr 
        115                 120                 125             


Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
    130                 135                 


<210>  40
<211>  129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD33 (h2H12) VlVh scFv

<400>  40

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Asp Ile Gln Met Thr Gln Ser Pro Ser Ser 
            20                  25                  30          


Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Asn Cys Lys Ala Ser 
        35                  40                  45              


Gln Asp Ile Asn Ser Tyr Leu Ser Trp Phe Gln Gln Lys Pro Gly Lys 
    50                  55                  60                  


Ala Pro Lys Thr Leu Ile Tyr Arg Ala Asn Arg Leu Val Asp Gly Val 
65                  70                  75                  80  


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Gln Asp Tyr Thr Leu Thr 
                85                  90                  95      


Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln 
            100                 105                 110         


Tyr Asp Glu Phe Pro Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile 
        115                 120                 125             


Lys 
    


<210>  41
<211>  150
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mesothelin (P4) scFv

<400>  41

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gln Val Gln Leu Gln Gln Ser Gly Pro Gly 
            20                  25                  30          


Leu Val Thr Pro Ser Gln Thr Leu Ser Leu Thr Cys Ala Ile Ser Gly 
        35                  40                  45              


Asp Ser Val Ser Ser Asn Ser Ala Thr Trp Asn Trp Ile Arg Gln Ser 
    50                  55                  60                  


Pro Ser Arg Gly Leu Glu Trp Leu Gly Arg Thr Tyr Tyr Arg Ser Lys 
65                  70                  75                  80  


Trp Tyr Asn Asp Tyr Ala Val Ser Val Lys Ser Arg Met Ser Ile Asn 
                85                  90                  95      


Pro Asp Thr Ser Lys Asn Gln Phe Ser Leu Gln Leu Asn Ser Val Thr 
            100                 105                 110         


Pro Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Gly Met Met Thr Tyr 
        115                 120                 125             


Tyr Tyr Gly Met Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser 
    130                 135                 140                 


Ser Gly Ile Leu Gly Ser 
145                 150 


<210>  42
<211>  448
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VAR2CSA (ID1-DBL2Xb) scFv

<400>  42

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Ser Leu Thr Asn Gly Tyr Lys Cys Asp Lys 
            20                  25                  30          


Cys Lys Ser Gly Thr Ser Arg Ser Lys Lys Lys Trp Ile Trp Lys Lys 
        35                  40                  45              


Ser Ser Gly Asn Glu Glu Gly Leu Gln Glu Glu Tyr Ala Asn Thr Ile 
    50                  55                  60                  


Gly Leu Pro Pro Arg Thr Gln Ser Leu Tyr Leu Gly Asn Leu Pro Lys 
65                  70                  75                  80  


Leu Glu Asn Val Cys Glu Asp Val Lys Asp Ile Asn Phe Asp Thr Lys 
                85                  90                  95      


Glu Lys Phe Leu Ala Gly Cys Leu Ile Val Ser Phe His Glu Gly Lys 
            100                 105                 110         


Asn Leu Lys Lys Arg Tyr Pro Gln Asn Lys Asn Ser Gly Asn Lys Glu 
        115                 120                 125             


Asn Leu Cys Lys Ala Leu Glu Tyr Ser Phe Ala Asp Tyr Gly Asp Leu 
    130                 135                 140                 


Ile Lys Gly Thr Ser Ile Trp Asp Asn Glu Tyr Thr Lys Asp Leu Glu 
145                 150                 155                 160 


Leu Asn Leu Gln Asn Asn Phe Gly Lys Leu Phe Gly Lys Tyr Ile Lys 
                165                 170                 175     


Lys Asn Asn Thr Ala Glu Gln Asp Thr Ser Tyr Ser Ser Leu Asp Glu 
            180                 185                 190         


Leu Arg Glu Ser Trp Trp Asn Thr Asn Lys Lys Tyr Ile Trp Thr Ala 
        195                 200                 205             


Met Lys His Gly Ala Glu Met Asn Ile Thr Thr Cys Asn Ala Asp Gly 
    210                 215                 220                 


Ser Val Thr Gly Ser Gly Ser Ser Cys Asp Asp Ile Pro Thr Ile Asp 
225                 230                 235                 240 


Leu Ile Pro Gln Tyr Leu Arg Phe Leu Gln Glu Trp Val Glu Asn Phe 
                245                 250                 255     


Cys Glu Gln Arg Gln Ala Lys Val Lys Asp Val Ile Thr Asn Cys Lys 
            260                 265                 270         


Ser Cys Lys Glu Ser Gly Asn Lys Cys Lys Thr Glu Cys Lys Thr Lys 
        275                 280                 285             


Cys Lys Asp Glu Cys Glu Lys Tyr Lys Lys Phe Ile Glu Ala Cys Gly 
    290                 295                 300                 


Thr Ala Gly Gly Gly Ile Gly Thr Ala Gly Ser Pro Trp Ser Lys Arg 
305                 310                 315                 320 


Trp Asp Gln Ile Tyr Lys Arg Tyr Ser Lys His Ile Glu Asp Ala Lys 
                325                 330                 335     


Arg Asn Arg Lys Ala Gly Thr Lys Asn Cys Gly Thr Ser Ser Thr Thr 
            340                 345                 350         


Asn Ala Ala Ala Ser Thr Asp Glu Asn Lys Cys Val Gln Ser Asp Ile 
        355                 360                 365             


Asp Ser Phe Phe Lys His Leu Ile Asp Ile Gly Leu Thr Thr Pro Ser 
    370                 375                 380                 


Ser Tyr Leu Ser Asn Val Leu Asp Asp Asn Ile Cys Gly Ala Asp Lys 
385                 390                 395                 400 


Ala Pro Trp Thr Thr Tyr Thr Thr Tyr Thr Thr Thr Glu Lys Cys Asn 
                405                 410                 415     


Lys Glu Arg Asp Lys Ser Lys Ser Gln Ser Ser Asp Thr Leu Val Val 
            420                 425                 430         


Val Asn Val Pro Ser Pro Leu Gly Asn Thr Pro Tyr Arg Tyr Lys Tyr 
        435                 440                 445             


<210>  43
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-IL13Ra2 (IL13 zetakine) amino acid

<400>  43

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gly Pro Val Pro Pro Ser Thr Ala Leu Arg 
            20                  25                  30          


Tyr Leu Ile Glu Glu Leu Val Asn Ile Thr Gln Asn Gln Lys Ala Pro 
        35                  40                  45              


Leu Cys Asn Gly Ser Met Val Trp Ser Ile Asn Leu Thr Ala Gly Met 
    50                  55                  60                  


Tyr Cys Ala Ala Leu Glu Ser Leu Ile Asn Val Ser Gly Cys Ser Ala 
65                  70                  75                  80  


Ile Glu Lys Thr Gln Arg Met Leu Ser Gly Phe Cys Pro His Lys Val 
                85                  90                  95      


Ser Ala Gly Gln Phe Ser Ser Leu His Val Arg Asp Thr Lys Ile Glu 
            100                 105                 110         


Val Ala Gln Phe Val Lys Asp Leu Leu Leu His Leu Lys Lys Leu Phe 
        115                 120                 125             


Arg Glu Gly Arg Phe 
    130             


<210>  44
<211>  251
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FITCE2 scFv

<400>  44

Ser Val Leu Thr Gln Pro Ser Ser Val Ser Ala Ala Pro Gly Gln Lys 
1               5                   10                  15      


Val Thr Ile Ser Cys Ser Gly Ser Thr Ser Asn Ile Gly Asn Asn Tyr 
            20                  25                  30          


Val Ser Trp Tyr Gln Gln His Pro Gly Lys Ala Pro Lys Leu Met Ile 
        35                  40                  45              


Tyr Asp Val Ser Lys Arg Pro Ser Gly Val Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  


Ser Lys Ser Gly Asn Ser Ala Ser Leu Asp Ile Ser Gly Leu Gln Ser 
65                  70                  75                  80  


Glu Asp Glu Ala Asp Tyr Tyr Cys Ala Ala Trp Asp Asp Ser Leu Ser 
                85                  90                  95      


Glu Phe Leu Phe Gly Thr Gly Thr Lys Leu Thr Val Leu Gly Gly Gly 
            100                 105                 110         


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln 
        115                 120                 125             


Leu Val Glu Ser Gly Gly Asn Leu Val Gln Pro Gly Gly Ser Leu Arg 
    130                 135                 140                 


Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Gly Ser Phe Ser Met Ser 
145                 150                 155                 160 


Trp Val Arg Gln Ala Pro Gly Gly Gly Leu Glu Trp Val Ala Gly Leu 
                165                 170                 175     


Ser Ala Arg Ser Ser Leu Thr His Tyr Ala Asp Ser Val Lys Gly Arg 
            180                 185                 190         


Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Val Tyr Leu Gln Met 
        195                 200                 205             


Asn Ser Leu Arg Val Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Arg 
    210                 215                 220                 


Ser Tyr Asp Ser Ser Gly Tyr Trp Gly His Phe Tyr Ser Tyr Met Asp 
225                 230                 235                 240 


Val Trp Gly Gln Gly Thr Leu Val Thr Val Ser 
                245                 250     


<210>  45
<211>  251
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FITCE2 TyrH133Ala scFv

<400>  45

Ser Val Leu Thr Gln Pro Ser Ser Val Ser Ala Ala Pro Gly Gln Lys 
1               5                   10                  15      


Val Thr Ile Ser Cys Ser Gly Ser Thr Ser Asn Ile Gly Asn Asn Tyr 
            20                  25                  30          


Val Ser Trp Tyr Gln Gln His Pro Gly Lys Ala Pro Lys Leu Met Ile 
        35                  40                  45              


Tyr Asp Val Ser Lys Arg Pro Ser Gly Val Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  


Ser Lys Ser Gly Asn Ser Ala Ser Leu Asp Ile Ser Gly Leu Gln Ser 
65                  70                  75                  80  


Glu Asp Glu Ala Asp Tyr Tyr Cys Ala Ala Trp Asp Asp Ser Leu Ser 
                85                  90                  95      


Glu Phe Leu Phe Gly Thr Gly Thr Lys Leu Thr Val Leu Gly Gly Gly 
            100                 105                 110         


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln 
        115                 120                 125             


Leu Val Glu Ser Gly Gly Asn Leu Val Gln Pro Gly Gly Ser Leu Arg 
    130                 135                 140                 


Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Gly Ser Phe Ser Met Ser 
145                 150                 155                 160 


Trp Val Arg Gln Ala Pro Gly Gly Gly Leu Glu Trp Val Ala Gly Leu 
                165                 170                 175     


Ser Ala Arg Ser Ser Leu Thr His Tyr Ala Asp Ser Val Lys Gly Arg 
            180                 185                 190         


Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Val Tyr Leu Gln Met 
        195                 200                 205             


Asn Ser Leu Arg Val Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Arg 
    210                 215                 220                 


Ser Tyr Asp Ser Ser Gly Tyr Trp Gly His Phe Ala Ser Tyr Met Asp 
225                 230                 235                 240 


Val Trp Gly Gln Gly Thr Leu Val Thr Val Ser 
                245                 250     


<210>  46
<211>  250
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FITCE2 HisH131Ala scFv

<400>  46

Val Leu Thr Gln Pro Ser Ser Val Ser Ala Ala Pro Gly Gln Lys Val 
1               5                   10                  15      


Thr Ile Ser Cys Ser Gly Ser Thr Ser Asn Ile Gly Asn Asn Tyr Val 
            20                  25                  30          


Ser Trp Tyr Gln Gln His Pro Gly Lys Ala Pro Lys Leu Met Ile Tyr 
        35                  40                  45              


Asp Val Ser Lys Arg Pro Ser Gly Val Pro Asp Arg Phe Ser Gly Ser 
    50                  55                  60                  


Lys Ser Gly Asn Ser Ala Ser Leu Asp Ile Ser Gly Leu Gln Ser Glu 
65                  70                  75                  80  


Asp Glu Ala Asp Tyr Tyr Cys Ala Ala Trp Asp Asp Ser Leu Ser Glu 
                85                  90                  95      


Phe Leu Phe Gly Thr Gly Thr Lys Leu Thr Val Leu Gly Gly Gly Gly 
            100                 105                 110         


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu 
        115                 120                 125             


Val Glu Ser Gly Gly Asn Leu Val Gln Pro Gly Gly Ser Leu Arg Leu 
    130                 135                 140                 


Ser Cys Ala Ala Ser Gly Phe Thr Phe Gly Ser Phe Ser Met Ser Trp 
145                 150                 155                 160 


Val Arg Gln Ala Pro Gly Gly Gly Leu Glu Trp Val Ala Gly Leu Ser 
                165                 170                 175     


Ala Arg Ser Ser Leu Thr His Tyr Ala Asp Ser Val Lys Gly Arg Phe 
            180                 185                 190         


Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Val Tyr Leu Gln Met Asn 
        195                 200                 205             


Ser Leu Arg Val Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Arg Ser 
    210                 215                 220                 


Tyr Asp Ser Ser Gly Tyr Trp Gly Ala Phe Tyr Ser Tyr Met Asp Val 
225                 230                 235                 240 


Trp Gly Gln Gly Thr Leu Val Thr Val Ser 
                245                 250 


<210>  47
<211>  254
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FL (4M5.3) scFv

<400>  47

Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu Arg Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Val Leu Ile Tyr Lys Val Ser Asn Arg Val Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Asn Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Ser 
                85                  90                  95      


Thr His Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Ser Ser Ala Asp Asp Ala Lys Lys Asp Ala Ala Lys Lys Asp Asp Ala 
        115                 120                 125             


Lys Lys Asp Asp Ala Lys Lys Asp Gly Gly Val Lys Leu Asp Glu Thr 
    130                 135                 140                 


Gly Gly Gly Leu Val Gln Pro Gly Gly Ala Met Lys Leu Ser Cys Val 
145                 150                 155                 160 


Thr Ser Gly Phe Thr Phe Gly His Tyr Trp Met Asn Trp Val Arg Gln 
                165                 170                 175     


Ser Pro Glu Lys Gly Leu Glu Trp Val Ala Gln Phe Arg Asn Lys Pro 
            180                 185                 190         


Tyr Asn Tyr Glu Thr Tyr Tyr Ser Asp Ser Val Lys Gly Arg Phe Thr 
        195                 200                 205             


Ile Ser Arg Asp Asp Ser Lys Ser Ser Val Tyr Leu Gln Met Asn Asn 
    210                 215                 220                 


Leu Arg Val Glu Asp Thr Gly Ile Tyr Tyr Cys Thr Gly Ala Ser Tyr 
225                 230                 235                 240 


Gly Met Glu Tyr Leu Gly Gln Gly Thr Ser Val Thr Val Ser 
                245                 250                 


<210>  48
<211>  265
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FL (4D5Flu) scFv

<400>  48

Asp Tyr Lys Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala 
1               5                   10                  15      


Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Leu 
            20                  25                  30          


Val His Ser Gln Gly Asn Thr Tyr Leu Arg Trp Tyr Gln Gln Lys Pro 
        35                  40                  45              


Gly Lys Ala Pro Lys Val Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser 
    50                  55                  60                  


Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr 
65                  70                  75                  80  


Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys 
                85                  90                  95      


Gln Gln Ser Thr His Val Pro Trp Thr Phe Gly Gln Gly Thr Lys Val 
            100                 105                 110         


Glu Leu Lys Arg Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
        115                 120                 125             


Gly Gly Gly Ser Ser Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln 
145                 150                 155                 160 


Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 
                165                 170                 175     


Ser Asp Tyr Trp Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
            180                 185                 190         


Glu Trp Val Ala Gln Ile Arg Asn Lys Pro Tyr Asn Tyr Glu Thr Tyr 
        195                 200                 205             


Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Thr Ser 
    210                 215                 220                 


Lys Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr 
225                 230                 235                 240 


Ala Val Tyr Tyr Cys Thr Gly Ser Tyr Tyr Gly Met Asp Tyr Trp Gly 
                245                 250                 255     


Gln Gly Thr Leu Val Thr Val Ser Ser 
            260                 265 


<210>  49
<211>  244
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FL (4420) scFv

<400>  49

Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Gln Gly Asn Thr Tyr Leu Arg Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Val Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Ser 
                85                  90                  95      


Thr His Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Gly 
            100                 105                 110         


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val 
        115                 120                 125             


Lys Leu Asp Glu Thr Gly Gly Gly Leu Val Gln Pro Gly Arg Pro Met 
    130                 135                 140                 


Lys Leu Ser Cys Val Ala Ser Gly Phe Thr Phe Ser Asp Tyr Trp Met 
145                 150                 155                 160 


Asn Trp Val Arg Gln Ser Pro Glu Lys Gly Leu Glu Trp Val Ala Gln 
                165                 170                 175     


Ile Arg Asn Lys Pro Tyr Asn Tyr Glu Thr Tyr Tyr Ser Asp Ser Val 
            180                 185                 190         


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Ser Ser Val Tyr 
        195                 200                 205             


Leu Gln Met Asn Asn Leu Arg Val Glu Asp Met Gly Ile Tyr Tyr Cys 
    210                 215                 220                 


Thr Gly Ser Tyr Tyr Gly Met Asp Tyr Trp Gly Gln Gly Thr Ser Val 
225                 230                 235                 240 


Thr Val Ser Ser 
                


<210>  50
<211>  789
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNP scFv (VH to VL from rabbit)

<400>  50
cagtgtcagc agctggagca gtccggagga ggagccgaag gaggcctggt caagcctggg       60

ggatccctgg aactctgctg caaagcctct ggattctccc tcagtagtag ctactgcata      120

tgttgggtcc gccaggctcc agggaagggg ctggagtgga tcggatgcat ttatgctggt      180

agtagtggta gcacttacta cgcgagctgg gtgaatggcc gattcactct ctccagagac      240

attgaccaga gcacaggttg cctacaactg aacagtctga cagccgcgga cacggccatg      300

tattactgtg cgagagcccc ctatagtagt ggctgggtcc tctactttaa cttgtggggc      360

ccaggcaccc tggtcattgt ctcctcaggc ggagggggct ctggcggcgg aggatctggg      420

ggagggggca gcccaggtgc cacatttgcc caagtgctga cccagactcc atcgcctgtg      480

tctgcagctg tgggaggcac agtcaccatc agttgccagt ccagtgagag tgtttatggt      540

aacagccgct tagcctggta tcagcagaaa ccagggcagt ctcccaagct cctgatctat      600

tatgcatcca ctctggcatc tggggtccct tcgcggttca aaggcagtgg atctgggaca      660

cagttcactc tcaccattag cgacctggag tgtgacgatg ctgcctctta ctactgtcaa      720

ggcggttatt atagtggtaa tcttgatgcg cttgctttcg gcggagggac cgaggtggtg      780

gtcagaggt                                                              789


<210>  51
<211>  263
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DNP scFv (VH to VL from rabbit) protein

<400>  51

Gln Cys Gln Gln Leu Glu Gln Ser Gly Gly Gly Ala Glu Gly Gly Leu 
1               5                   10                  15      


Val Lys Pro Gly Gly Ser Leu Glu Leu Cys Cys Lys Ala Ser Gly Phe 
            20                  25                  30          


Ser Leu Ser Ser Ser Tyr Cys Ile Cys Trp Val Arg Gln Ala Pro Gly 
        35                  40                  45              


Lys Gly Leu Glu Trp Ile Gly Cys Ile Tyr Ala Gly Ser Ser Gly Ser 
    50                  55                  60                  


Thr Tyr Tyr Ala Ser Trp Val Asn Gly Arg Phe Thr Leu Ser Arg Asp 
65                  70                  75                  80  


Ile Asp Gln Ser Thr Gly Cys Leu Gln Leu Asn Ser Leu Thr Ala Ala 
                85                  90                  95      


Asp Thr Ala Met Tyr Tyr Cys Ala Arg Ala Pro Tyr Ser Ser Gly Trp 
            100                 105                 110         


Val Leu Tyr Phe Asn Leu Trp Gly Pro Gly Thr Leu Val Ile Val Ser 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Pro Gly Ala Thr Phe Ala Gln Val Leu Thr Gln Thr Pro Ser Pro Val 
145                 150                 155                 160 


Ser Ala Ala Val Gly Gly Thr Val Thr Ile Ser Cys Gln Ser Ser Glu 
                165                 170                 175     


Ser Val Tyr Gly Asn Ser Arg Leu Ala Trp Tyr Gln Gln Lys Pro Gly 
            180                 185                 190         


Gln Ser Pro Lys Leu Leu Ile Tyr Tyr Ala Ser Thr Leu Ala Ser Gly 
        195                 200                 205             


Val Pro Ser Arg Phe Lys Gly Ser Gly Ser Gly Thr Gln Phe Thr Leu 
    210                 215                 220                 


Thr Ile Ser Asp Leu Glu Cys Asp Asp Ala Ala Ser Tyr Tyr Cys Gln 
225                 230                 235                 240 


Gly Gly Tyr Tyr Ser Gly Asn Leu Asp Ala Leu Ala Phe Gly Gly Gly 
                245                 250                 255     


Thr Glu Val Val Val Arg Gly 
            260             


<210>  52
<211>  789
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNP scFv (VL to VH from rabbit)

<400>  52
ccaggtgcca catttgccca agtgctgacc cagactccat cgcctgtgtc tgcagctgtg       60

ggaggcacag tcaccatcag ttgccagtcc agtgagagtg tttatggtaa cagccgctta      120

gcctggtatc agcagaaacc agggcagtct cccaagctcc tgatctatta tgcatccact      180

ctggcatctg gggtcccttc gcggttcaaa ggcagtggat ctgggacaca gttcactctc      240

accattagcg acctggagtg tgacgatgct gcctcttact actgtcaagg cggttattat      300

agtggtaatc ttgatgcgct tgctttcggc ggagggaccg aggtggtggt cagaggtggc      360

ggagggggct ctggcggcgg aggatctggg ggagggggca gccagtgtca gcagctggag      420

cagtccggag gaggagccga aggaggcctg gtcaagcctg ggggatccct ggaactctgc      480

tgcaaagcct ctggattctc cctcagtagt agctactgca tatgttgggt ccgccaggct      540

ccagggaagg ggctggagtg gatcggatgc atttatgctg gtagtagtgg tagcacttac      600

tacgcgagct gggtgaatgg ccgattcact ctctccagag acattgacca gagcacaggt      660

tgcctacaac tgaacagtct gacagccgcg gacacggcca tgtattactg tgcgagagcc      720

ccctatagta gtggctgggt cctctacttt aacttgtggg gcccaggcac cctggtcatt      780

gtctcctca                                                              789


<210>  53
<211>  263
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DNP scFv (VL to VH from rabbit) protein

<400>  53

Pro Gly Ala Thr Phe Ala Gln Val Leu Thr Gln Thr Pro Ser Pro Val 
1               5                   10                  15      


Ser Ala Ala Val Gly Gly Thr Val Thr Ile Ser Cys Gln Ser Ser Glu 
            20                  25                  30          


Ser Val Tyr Gly Asn Ser Arg Leu Ala Trp Tyr Gln Gln Lys Pro Gly 
        35                  40                  45              


Gln Ser Pro Lys Leu Leu Ile Tyr Tyr Ala Ser Thr Leu Ala Ser Gly 
    50                  55                  60                  


Val Pro Ser Arg Phe Lys Gly Ser Gly Ser Gly Thr Gln Phe Thr Leu 
65                  70                  75                  80  


Thr Ile Ser Asp Leu Glu Cys Asp Asp Ala Ala Ser Tyr Tyr Cys Gln 
                85                  90                  95      


Gly Gly Tyr Tyr Ser Gly Asn Leu Asp Ala Leu Ala Phe Gly Gly Gly 
            100                 105                 110         


Thr Glu Val Val Val Arg Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Gln Cys Gln Gln Leu Glu Gln Ser Gly Gly 
    130                 135                 140                 


Gly Ala Glu Gly Gly Leu Val Lys Pro Gly Gly Ser Leu Glu Leu Cys 
145                 150                 155                 160 


Cys Lys Ala Ser Gly Phe Ser Leu Ser Ser Ser Tyr Cys Ile Cys Trp 
                165                 170                 175     


Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile Gly Cys Ile Tyr 
            180                 185                 190         


Ala Gly Ser Ser Gly Ser Thr Tyr Tyr Ala Ser Trp Val Asn Gly Arg 
        195                 200                 205             


Phe Thr Leu Ser Arg Asp Ile Asp Gln Ser Thr Gly Cys Leu Gln Leu 
    210                 215                 220                 


Asn Ser Leu Thr Ala Ala Asp Thr Ala Met Tyr Tyr Cys Ala Arg Ala 
225                 230                 235                 240 


Pro Tyr Ser Ser Gly Trp Val Leu Tyr Phe Asn Leu Trp Gly Pro Gly 
                245                 250                 255     


Thr Leu Val Ile Val Ser Ser 
            260             


<210>  54
<211>  217
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH of DNP scFv (Ab-1; 1BAF)

<400>  54

Asp Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Ser Gln Ser Leu Thr Cys Thr Val Thr Gly Tyr Ser Ile Thr Ser Asp 
            20                  25                  30          


Tyr Ala Trp Asn Trp Ile Arg Gln Phe Pro Gly Asn Lys Leu Glu Trp 
        35                  40                  45              


Met Gly Tyr Met Ser Tyr Ser Gly Ser Thr Arg Tyr Asn Pro Ser Leu 
    50                  55                  60                  


Arg Ser Arg Ile Ser Ile Thr Arg Asp Thr Ser Lys Asn Gln Phe Phe 
65                  70                  75                  80  


Leu Gln Leu Lys Ser Val Thr Thr Glu Asp Thr Ala Thr Tyr Phe Cys 
                85                  90                  95      


Ala Arg Gly Trp Pro Leu Ala Tyr Trp Gly Gln Gly Thr Gln Val Ser 
            100                 105                 110         


Val Ser Glu Ala Lys Thr Thr Pro Pro Ser Val Tyr Pro Leu Ala Pro 
        115                 120                 125             


Gly Ser Ala Ala Gln Thr Asn Ser Met Val Thr Leu Gly Cys Leu Val 
    130                 135                 140                 


Lys Gly Tyr Phe Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ser 
145                 150                 155                 160 


Leu Ser Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Asp Leu 
                165                 170                 175     


Tyr Thr Leu Ser Ser Ser Val Thr Val Pro Ser Ser Pro Arg Pro Ser 
            180                 185                 190         


Glu Thr Val Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val 
        195                 200                 205             


Asp Lys Lys Ile Val Pro Arg Asp Cys 
    210                 215         


<210>  55
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL of DNP scFv (Ab-1; 1BAF)

<400>  55

Gln Ile Val Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Thr Cys Ser Ala Ser Ser Ser Val Tyr Tyr Met 
            20                  25                  30          


Tyr Trp Tyr Gln Gln Lys Pro Gly Ser Ser Pro Arg Leu Leu Ile Tyr 
        35                  40                  45              


Asp Thr Ser Asn Leu Ala Ser Gly Val Pro Val Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg Met Glu Ala Glu 
65                  70                  75                  80  


Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Tyr Pro Pro Ile 
                85                  90                  95      


Thr Phe Gly Val Gly Thr Lys Leu Glu Leu Lys Arg Ala Asp Ala Ala 
            100                 105                 110         


Pro Thr Val Ser Ile Phe Pro Pro Ser Ser Glu Gln Leu Thr Ser Gly 
        115                 120                 125             


Gly Ala Ser Val Val Cys Phe Leu Asn Asn Phe Tyr Pro Lys Asp Ile 
    130                 135                 140                 


Asn Val Lys Trp Lys Ile Asp Gly Ser Glu Arg Gln Asn Gly Val Leu 
145                 150                 155                 160 


Asn Ser Trp Thr Asp Gln Asp Ser Lys Asp Ser Thr Tyr Ser Met Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Thr Lys Asp Glu Tyr Glu Arg His Asn Ser Tyr 
            180                 185                 190         


Thr Cys Glu Ala Thr His Lys Thr Ser Thr Ser Pro Ile Val Lys Ser 
        195                 200                 205             


Phe Asn Arg Asn Glu Cys 
    210                 


<210>  56
<211>  125
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH of DNP scFv (Ab-2; XC)

<400>  56

Gln Gln Leu Glu Gln Ser Gly Gly Gly Ala Glu Gly Gly Leu Val Lys 
1               5                   10                  15      


Pro Gly Gly Ser Leu Glu Leu Cys Cys Lys Ala Ser Gly Phe Ser Leu 
            20                  25                  30          


Ser Ser Ser Tyr Cys Ile Cys Trp Val Arg Gln Ala Pro Gly Lys Gly 
        35                  40                  45              


Leu Glu Trp Ile Gly Cys Ile Tyr Ala Gly Ser Ser Gly Ser Thr Tyr 
    50                  55                  60                  


Tyr Ala Ser Trp Val Asn Gly Arg Phe Thr Leu Ser Arg Asp Ile Asp 
65                  70                  75                  80  


Gln Ser Thr Gly Cys Leu Gln Leu Asn Ser Leu Thr Ala Ala Asp Thr 
                85                  90                  95      


Ala Met Tyr Tyr Cys Ala Arg Ala Pro Tyr Ser Ser Gly Trp Val Leu 
            100                 105                 110         


Tyr Phe Asn Leu Trp Gly Pro Gly Thr Leu Val Ile Val 
        115                 120                 125 


<210>  57
<211>  104
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL of DNP scFv (Ab-2; XC)

<400>  57

Ala Gln Val Leu Thr Gln Thr Pro Ser Pro Val Ser Ala Ala Val Gly 
1               5                   10                  15      


Gly Thr Val Thr Ile Ser Cys Gln Ser Ser Glu Ser Val Tyr Gly Asn 
            20                  25                  30          


Ser Arg Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu 
        35                  40                  45              


Leu Ile Tyr Tyr Ala Ser Thr Leu Ala Ser Gly Val Pro Ser Arg Phe 
    50                  55                  60                  


Lys Gly Ser Gly Ser Gly Thr Gln Phe Thr Leu Thr Ile Ser Asp Leu 
65                  70                  75                  80  


Glu Cys Asp Asp Ala Ala Ser Tyr Tyr Cys Gln Gly Gly Tyr Tyr Ser 
                85                  90                  95      


Gly Asn Leu Asp Ala Leu Ala Phe 
            100                 


<210>  58
<211>  125
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH of DNP scFv (Ab-3)

<400>  58

Gln Gln Leu Glu Gln Ser Gly Gly Gly Ala Glu Gly Gly Leu Val Lys 
1               5                   10                  15      


Pro Gly Gly Ser Leu Glu Leu Cys Cys Lys Ala Ser Gly Phe Ser Leu 
            20                  25                  30          


Ser Ser Ser Tyr Cys Ile Cys Trp Val Arg Gln Ala Pro Gly Lys Gly 
        35                  40                  45              


Leu Glu Trp Ile Gly Cys Ile Tyr Ala Gly Ser Ser Gly Ser Thr Tyr 
    50                  55                  60                  


Tyr Ala Ser Trp Val Asn Gly Arg Phe Thr Leu Ser Arg Asp Ile Asp 
65                  70                  75                  80  


Gln Ser Thr Gly Cys Leu Gln Leu Asn Ser Leu Thr Ala Ala Asp Thr 
                85                  90                  95      


Ala Met Tyr Tyr Cys Ala Arg Ala Pro Tyr Ser Ser Gly Trp Val Leu 
            100                 105                 110         


Tyr Phe Asn Leu Trp Gly Pro Gly Thr Leu Val Ile Val 
        115                 120                 125 


<210>  59
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL of DNP scFv (Ab-3)

<400>  59

Pro Gly Ala Thr Phe Ala Gln Val Leu Thr Gln Thr Pro Ser Pro Val 
1               5                   10                  15      


Ser Ala Ala Val Gly Gly Thr Val Thr Ile Ser Cys Gln Ser Ser Glu 
            20                  25                  30          


Ser Val Tyr Gly Asn Ser Arg Leu Ala Trp Tyr Gln Gln Lys Pro Gly 
        35                  40                  45              


Gln Ser Pro Lys Leu Leu Ile Tyr Tyr Ala Ser Thr Leu Ala Ser Gly 
    50                  55                  60                  


Val Pro Ser Arg Phe Lys Gly Ser Gly Ser Gly Thr Gln Phe Thr Leu 
65                  70                  75                  80  


Thr Ile Ser Asp Leu Glu Cys Asp Asp Ala Ala Ser Tyr Tyr Cys Gln 
                85                  90                  95      


Gly Gly Tyr Tyr Ser Gly Asn Leu Asp Ala Leu Ala Phe Gly Gly Gly 
            100                 105                 110         


Thr Glu Val Val Val Arg Gly 
        115                 


<210>  60
<211>  263
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL-linker-VH of DNP scFv (Ab-3)

<400>  60

Pro Gly Ala Thr Phe Ala Gln Val Leu Thr Gln Thr Pro Ser Pro Val 
1               5                   10                  15      


Ser Ala Ala Val Gly Gly Thr Val Thr Ile Ser Cys Gln Ser Ser Glu 
            20                  25                  30          


Ser Val Tyr Gly Asn Ser Arg Leu Ala Trp Tyr Gln Gln Lys Pro Gly 
        35                  40                  45              


Gln Ser Pro Lys Leu Leu Ile Tyr Tyr Ala Ser Thr Leu Ala Ser Gly 
    50                  55                  60                  


Val Pro Ser Arg Phe Lys Gly Ser Gly Ser Gly Thr Gln Phe Thr Leu 
65                  70                  75                  80  


Thr Ile Ser Asp Leu Glu Cys Asp Asp Ala Ala Ser Tyr Tyr Cys Gln 
                85                  90                  95      


Gly Gly Tyr Tyr Ser Gly Asn Leu Asp Ala Leu Ala Phe Gly Gly Gly 
            100                 105                 110         


Thr Glu Val Val Val Arg Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Gln Cys Gln Gln Leu Glu Gln Ser Gly Gly 
    130                 135                 140                 


Gly Ala Glu Gly Gly Leu Val Lys Pro Gly Gly Ser Leu Glu Leu Cys 
145                 150                 155                 160 


Cys Lys Ala Ser Gly Phe Ser Leu Ser Ser Ser Tyr Cys Ile Cys Trp 
                165                 170                 175     


Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile Gly Cys Ile Tyr 
            180                 185                 190         


Ala Gly Ser Ser Gly Ser Thr Tyr Tyr Ala Ser Trp Val Asn Gly Arg 
        195                 200                 205             


Phe Thr Leu Ser Arg Asp Ile Asp Gln Ser Thr Gly Cys Leu Gln Leu 
    210                 215                 220                 


Asn Ser Leu Thr Ala Ala Asp Thr Ala Met Tyr Tyr Cys Ala Arg Ala 
225                 230                 235                 240 


Pro Tyr Ser Ser Gly Trp Val Leu Tyr Phe Asn Leu Trp Gly Pro Gly 
                245                 250                 255     


Thr Leu Val Ile Val Ser Ser 
            260             


<210>  61
<211>  263
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-linker-VL of DNP scFv (Ab-3)

<400>  61

Gln Cys Gln Gln Leu Glu Gln Ser Gly Gly Gly Ala Glu Gly Gly Leu 
1               5                   10                  15      


Val Lys Pro Gly Gly Ser Leu Glu Leu Cys Cys Lys Ala Ser Gly Phe 
            20                  25                  30          


Ser Leu Ser Ser Ser Tyr Cys Ile Cys Trp Val Arg Gln Ala Pro Gly 
        35                  40                  45              


Lys Gly Leu Glu Trp Ile Gly Cys Ile Tyr Ala Gly Ser Ser Gly Ser 
    50                  55                  60                  


Thr Tyr Tyr Ala Ser Trp Val Asn Gly Arg Phe Thr Leu Ser Arg Asp 
65                  70                  75                  80  


Ile Asp Gln Ser Thr Gly Cys Leu Gln Leu Asn Ser Leu Thr Ala Ala 
                85                  90                  95      


Asp Thr Ala Met Tyr Tyr Cys Ala Arg Ala Pro Tyr Ser Ser Gly Trp 
            100                 105                 110         


Val Leu Tyr Phe Asn Leu Trp Gly Pro Gly Thr Leu Val Ile Val Ser 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Pro Gly Ala Thr Phe Ala Gln Val Leu Thr Gln Thr Pro Ser Pro Val 
145                 150                 155                 160 


Ser Ala Ala Val Gly Gly Thr Val Thr Ile Ser Cys Gln Ser Ser Glu 
                165                 170                 175     


Ser Val Tyr Gly Asn Ser Arg Leu Ala Trp Tyr Gln Gln Lys Pro Gly 
            180                 185                 190         


Gln Ser Pro Lys Leu Leu Ile Tyr Tyr Ala Ser Thr Leu Ala Ser Gly 
        195                 200                 205             


Val Pro Ser Arg Phe Lys Gly Ser Gly Ser Gly Thr Gln Phe Thr Leu 
    210                 215                 220                 


Thr Ile Ser Asp Leu Glu Cys Asp Asp Ala Ala Ser Tyr Tyr Cys Gln 
225                 230                 235                 240 


Gly Gly Tyr Tyr Ser Gly Asn Leu Asp Ala Leu Ala Phe Gly Gly Gly 
                245                 250                 255     


Thr Glu Val Val Val Arg Gly 
            260             


<210>  62
<211>  446
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-linker-VL of DNP scFv (1BAF)

<400>  62

Asp Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Ser Gln Ser Leu Thr Cys Thr Val Thr Gly Tyr Ser Ile Thr Ser Asp 
            20                  25                  30          


Tyr Ala Trp Asn Trp Ile Arg Gln Phe Pro Gly Asn Lys Leu Glu Trp 
        35                  40                  45              


Met Gly Tyr Met Ser Tyr Ser Gly Ser Thr Arg Tyr Asn Pro Ser Leu 
    50                  55                  60                  


Arg Ser Arg Ile Ser Ile Thr Arg Asp Thr Ser Lys Asn Gln Phe Phe 
65                  70                  75                  80  


Leu Gln Leu Lys Ser Val Thr Thr Glu Asp Thr Ala Thr Tyr Phe Cys 
                85                  90                  95      


Ala Arg Gly Trp Pro Leu Ala Tyr Trp Gly Gln Gly Thr Gln Val Ser 
            100                 105                 110         


Val Ser Glu Ala Lys Thr Thr Pro Pro Ser Val Tyr Pro Leu Ala Pro 
        115                 120                 125             


Gly Ser Ala Ala Gln Thr Asn Ser Met Val Thr Leu Gly Cys Leu Val 
    130                 135                 140                 


Lys Gly Tyr Phe Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ser 
145                 150                 155                 160 


Leu Ser Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Asp Leu 
                165                 170                 175     


Tyr Thr Leu Ser Ser Ser Val Thr Val Pro Ser Ser Pro Arg Pro Ser 
            180                 185                 190         


Glu Thr Val Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val 
        195                 200                 205             


Asp Lys Lys Ile Val Pro Arg Asp Cys Gly Gly Gly Gly Ser Gly Gly 
    210                 215                 220                 


Gly Gly Ser Gly Gly Gly Gly Ser Gln Ile Val Leu Thr Gln Ser Pro 
225                 230                 235                 240 


Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Ser 
                245                 250                 255     


Ala Ser Ser Ser Val Tyr Tyr Met Tyr Trp Tyr Gln Gln Lys Pro Gly 
            260                 265                 270         


Ser Ser Pro Arg Leu Leu Ile Tyr Asp Thr Ser Asn Leu Ala Ser Gly 
        275                 280                 285             


Val Pro Val Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu 
    290                 295                 300                 


Thr Ile Ser Arg Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln 
305                 310                 315                 320 


Gln Trp Ser Ser Tyr Pro Pro Ile Thr Phe Gly Val Gly Thr Lys Leu 
                325                 330                 335     


Glu Leu Lys Arg Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro 
            340                 345                 350         


Ser Ser Glu Gln Leu Thr Ser Gly Gly Ala Ser Val Val Cys Phe Leu 
        355                 360                 365             


Asn Asn Phe Tyr Pro Lys Asp Ile Asn Val Lys Trp Lys Ile Asp Gly 
    370                 375                 380                 


Ser Glu Arg Gln Asn Gly Val Leu Asn Ser Trp Thr Asp Gln Asp Ser 
385                 390                 395                 400 


Lys Asp Ser Thr Tyr Ser Met Ser Ser Thr Leu Thr Leu Thr Lys Asp 
                405                 410                 415     


Glu Tyr Glu Arg His Asn Ser Tyr Thr Cys Glu Ala Thr His Lys Thr 
            420                 425                 430         


Ser Thr Ser Pro Ile Val Lys Ser Phe Asn Arg Asn Glu Cys 
        435                 440                 445     


<210>  63
<211>  446
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL-linker-VH of DNP scFv (1BAF)

<400>  63

Gln Ile Val Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Thr Cys Ser Ala Ser Ser Ser Val Tyr Tyr Met 
            20                  25                  30          


Tyr Trp Tyr Gln Gln Lys Pro Gly Ser Ser Pro Arg Leu Leu Ile Tyr 
        35                  40                  45              


Asp Thr Ser Asn Leu Ala Ser Gly Val Pro Val Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg Met Glu Ala Glu 
65                  70                  75                  80  


Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Tyr Pro Pro Ile 
                85                  90                  95      


Thr Phe Gly Val Gly Thr Lys Leu Glu Leu Lys Arg Ala Asp Ala Ala 
            100                 105                 110         


Pro Thr Val Ser Ile Phe Pro Pro Ser Ser Glu Gln Leu Thr Ser Gly 
        115                 120                 125             


Gly Ala Ser Val Val Cys Phe Leu Asn Asn Phe Tyr Pro Lys Asp Ile 
    130                 135                 140                 


Asn Val Lys Trp Lys Ile Asp Gly Ser Glu Arg Gln Asn Gly Val Leu 
145                 150                 155                 160 


Asn Ser Trp Thr Asp Gln Asp Ser Lys Asp Ser Thr Tyr Ser Met Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Thr Lys Asp Glu Tyr Glu Arg His Asn Ser Tyr 
            180                 185                 190         


Thr Cys Glu Ala Thr His Lys Thr Ser Thr Ser Pro Ile Val Lys Ser 
        195                 200                 205             


Phe Asn Arg Asn Glu Cys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    210                 215                 220                 


Gly Gly Gly Gly Ser Asp Val Gln Leu Gln Glu Ser Gly Pro Gly Leu 
225                 230                 235                 240 


Val Lys Pro Ser Gln Ser Gln Ser Leu Thr Cys Thr Val Thr Gly Tyr 
                245                 250                 255     


Ser Ile Thr Ser Asp Tyr Ala Trp Asn Trp Ile Arg Gln Phe Pro Gly 
            260                 265                 270         


Asn Lys Leu Glu Trp Met Gly Tyr Met Ser Tyr Ser Gly Ser Thr Arg 
        275                 280                 285             


Tyr Asn Pro Ser Leu Arg Ser Arg Ile Ser Ile Thr Arg Asp Thr Ser 
    290                 295                 300                 


Lys Asn Gln Phe Phe Leu Gln Leu Lys Ser Val Thr Thr Glu Asp Thr 
305                 310                 315                 320 


Ala Thr Tyr Phe Cys Ala Arg Gly Trp Pro Leu Ala Tyr Trp Gly Gln 
                325                 330                 335     


Gly Thr Gln Val Ser Val Ser Glu Ala Lys Thr Thr Pro Pro Ser Val 
            340                 345                 350         


Tyr Pro Leu Ala Pro Gly Ser Ala Ala Gln Thr Asn Ser Met Val Thr 
        355                 360                 365             


Leu Gly Cys Leu Val Lys Gly Tyr Phe Pro Glu Pro Val Thr Val Thr 
    370                 375                 380                 


Trp Asn Ser Gly Ser Leu Ser Ser Gly Val His Thr Phe Pro Ala Val 
385                 390                 395                 400 


Leu Gln Ser Asp Leu Tyr Thr Leu Ser Ser Ser Val Thr Val Pro Ser 
                405                 410                 415     


Ser Pro Arg Pro Ser Glu Thr Val Thr Cys Asn Val Ala His Pro Ala 
            420                 425                 430         


Ser Ser Thr Lys Val Asp Lys Lys Ile Val Pro Arg Asp Cys 
        435                 440                 445     


<210>  64
<211>  246
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-linker-VL of DNP scFv (XC)

<400>  64

Gln Gln Leu Glu Gln Ser Gly Gly Gly Ala Glu Gly Gly Leu Val Lys 
1               5                   10                  15      


Pro Gly Gly Ser Leu Glu Leu Cys Cys Lys Ala Ser Gly Phe Ser Leu 
            20                  25                  30          


Ser Ser Ser Tyr Cys Ile Cys Trp Val Arg Gln Ala Pro Gly Lys Gly 
        35                  40                  45              


Leu Glu Trp Ile Gly Cys Ile Tyr Ala Gly Ser Ser Gly Ser Thr Tyr 
    50                  55                  60                  


Tyr Ala Ser Trp Val Asn Gly Arg Phe Thr Leu Ser Arg Asp Ile Asp 
65                  70                  75                  80  


Gln Ser Thr Gly Cys Leu Gln Leu Asn Ser Leu Thr Ala Ala Asp Thr 
                85                  90                  95      


Ala Met Tyr Tyr Cys Ala Arg Ala Pro Tyr Ser Ser Gly Trp Val Leu 
            100                 105                 110         


Tyr Phe Asn Leu Trp Gly Pro Gly Thr Leu Val Ile Val Ser Ser Gly 
        115                 120                 125             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Gln 
    130                 135                 140                 


Val Leu Thr Gln Thr Pro Ser Pro Val Ser Ala Ala Val Gly Gly Thr 
145                 150                 155                 160 


Val Thr Ile Ser Cys Gln Ser Ser Glu Ser Val Tyr Gly Asn Ser Arg 
                165                 170                 175     


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile 
            180                 185                 190         


Tyr Tyr Ala Ser Thr Leu Ala Ser Gly Val Pro Ser Arg Phe Lys Gly 
        195                 200                 205             


Ser Gly Ser Gly Thr Gln Phe Thr Leu Thr Ile Ser Asp Leu Glu Cys 
    210                 215                 220                 


Asp Asp Ala Ala Ser Tyr Tyr Cys Gln Gly Gly Tyr Tyr Ser Gly Asn 
225                 230                 235                 240 


Leu Asp Ala Leu Ala Phe 
                245     


<210>  65
<211>  254
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL-linker-VH of DNP scFv (XC)

<400>  65

Ala Gln Val Leu Thr Gln Thr Pro Ser Pro Val Ser Ala Ala Val Gly 
1               5                   10                  15      


Gly Thr Val Thr Ile Ser Cys Gln Ser Ser Glu Ser Val Tyr Gly Asn 
            20                  25                  30          


Ser Arg Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu 
        35                  40                  45              


Leu Ile Tyr Tyr Ala Ser Thr Leu Ala Ser Gly Val Pro Ser Arg Phe 
    50                  55                  60                  


Lys Gly Ser Gly Ser Gly Thr Gln Phe Thr Leu Thr Ile Ser Asp Leu 
65                  70                  75                  80  


Glu Cys Asp Asp Ala Ala Ser Tyr Tyr Cys Gln Gly Gly Tyr Tyr Ser 
                85                  90                  95      


Gly Asn Leu Asp Ala Leu Ala Phe Gly Gly Gly Thr Glu Val Val Val 
            100                 105                 110         


Arg Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Gln Gln Leu Glu Gln Ser Gly Gly Gly Ala Glu Gly Gly Leu Val 
    130                 135                 140                 


Lys Pro Gly Gly Ser Leu Glu Leu Cys Cys Lys Ala Ser Gly Phe Ser 
145                 150                 155                 160 


Leu Ser Ser Ser Tyr Cys Ile Cys Trp Val Arg Gln Ala Pro Gly Lys 
                165                 170                 175     


Gly Leu Glu Trp Ile Gly Cys Ile Tyr Ala Gly Ser Ser Gly Ser Thr 
            180                 185                 190         


Tyr Tyr Ala Ser Trp Val Asn Gly Arg Phe Thr Leu Ser Arg Asp Ile 
        195                 200                 205             


Asp Gln Ser Thr Gly Cys Leu Gln Leu Asn Ser Leu Thr Ala Ala Asp 
    210                 215                 220                 


Thr Ala Met Tyr Tyr Cys Ala Arg Ala Pro Tyr Ser Ser Gly Trp Val 
225                 230                 235                 240 


Leu Tyr Phe Asn Leu Trp Gly Pro Gly Thr Leu Val Ile Val 
                245                 250                 


<210>  66
<211>  1338
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VH-linker-VL of DNP scFv (1BAF) coding sequence

<400>  66
gacgtacaac tgcaagaatc aggtccggga ctggtcaaac ctagccagtc tcagtccttg       60

acgtgcaccg tgacgggcta tagtataacg agtgattacg cttggaattg gattcggcag      120

tttccaggca ataagcttga gtggatgggc tatatgtctt actccggctc aactaggtat      180

aatccgagcc ttcggtctcg gatttctatc acaagagata cgagtaagaa tcagtttttt      240

cttcaactca aaagcgtcac cacggaggat accgccacat atttctgtgc taggggatgg      300

cctcttgctt attgggggca agggacacaa gtgtctgttt ccgaagccaa aacaacgccc      360

ccttcagtct atccgctggc accgggaagc gcggcacaaa ccaatagtat ggtaacgctc      420

ggatgtctcg tcaaggggta ttttcccgag cctgtgacag tgacatggaa ttctgggagt      480

cttagcagcg gagtacatac ttttccggca gtacttcaat ccgatttgta cacgctctcc      540

tctagtgtta cagttccaag ctctccacga cctagtgaga ccgttacatg taacgtcgcg      600

catccggcct cttccactaa agtggataaa aagattgtgc ccagggactg cggcggaggg      660

ggctctggcg gcggaggatc tgggggaggg ggcagccaaa ttgtgttgac ccagtccccg      720

gccataatgt ccgcttctcc tggcgagaag gttactatga cttgctcagc ctcctccagt      780

gtgtattata tgtactggta tcaacaaaag ccgggctctt ccccccggct ccttatatac      840

gacacgagta atctggcaag tggcgtgcct gttagatttt ctgggtccgg ctctggaact      900

tcatactccc tgacaattag ccgaatggaa gccgaggacg cggcgacata ctactgccag      960

caatggtcat cctatccgcc tatcactttt ggagtaggga ccaaattgga gttgaagcgg     1020

gctgatgcgg ctcccacagt tagtattttc cctccgtcca gtgaacaact tacctccggg     1080

ggagcctccg ttgtttgctt tctgaacaac ttttacccga aagatataaa tgtcaagtgg     1140

aagatcgacg gctcagagcg ccaaaacggg gtactcaact catggacaga tcaggatagt     1200

aaagattcaa cttacagtat gtctagtacc ctgacactga cgaaagatga atacgaaaga     1260

cataatagtt atacctgtga agctacacat aagacttcaa cctctcctat tgtaaaatca     1320

ttcaaccgaa acgaatgt                                                   1338


<210>  67
<211>  1338
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VL-linker-VH of DNP scFv (1BAF) coding sequence

<400>  67
caaattgtgt tgacccagtc cccggccata atgtccgctt ctcctggcga gaaggttact       60

atgacttgct cagcctcctc cagtgtgtat tatatgtact ggtatcaaca aaagccgggc      120

tcttcccccc ggctccttat atacgacacg agtaatctgg caagtggcgt gcctgttaga      180

ttttctgggt ccggctctgg aacttcatac tccctgacaa ttagccgaat ggaagccgag      240

gacgcggcga catactactg ccagcaatgg tcatcctatc cgcctatcac ttttggagta      300

gggaccaaat tggagttgaa gcgggctgat gcggctccca cagttagtat tttccctccg      360

tccagtgaac aacttacctc cgggggagcc tccgttgttt gctttctgaa caacttttac      420

ccgaaagata taaatgtcaa gtggaagatc gacggctcag agcgccaaaa cggggtactc      480

aactcatgga cagatcagga tagtaaagat tcaacttaca gtatgtctag taccctgaca      540

ctgacgaaag atgaatacga aagacataat agttatacct gtgaagctac acataagact      600

tcaacctctc ctattgtaaa atcattcaac cgaaacgaat gtggcggagg gggctctggc      660

ggcggaggat ctgggggagg gggcagcgac gtacaactgc aagaatcagg tccgggactg      720

gtcaaaccta gccagtctca gtccttgacg tgcaccgtga cgggctatag tataacgagt      780

gattacgctt ggaattggat tcggcagttt ccaggcaata agcttgagtg gatgggctat      840

atgtcttact ccggctcaac taggtataat ccgagccttc ggtctcggat ttctatcaca      900

agagatacga gtaagaatca gttttttctt caactcaaaa gcgtcaccac ggaggatacc      960

gccacatatt tctgtgctag gggatggcct cttgcttatt gggggcaagg gacacaagtg     1020

tctgtttccg aagccaaaac aacgccccct tcagtctatc cgctggcacc gggaagcgcg     1080

gcacaaacca atagtatggt aacgctcgga tgtctcgtca aggggtattt tcccgagcct     1140

gtgacagtga catggaattc tgggagtctt agcagcggag tacatacttt tccggcagta     1200

cttcaatccg atttgtacac gctctcctct agtgttacag ttccaagctc tccacgacct     1260

agtgagaccg ttacatgtaa cgtcgcgcat ccggcctctt ccactaaagt ggataaaaag     1320

attgtgccca gggactgc                                                   1338


<210>  68
<211>  738
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VH-linker-VL of DNP scFv (XC) coding sequence

<400>  68
cagcagctgg agcagtccgg aggaggagcc gaaggaggcc tggtcaagcc tgggggatcc       60

ctggaactct gctgcaaagc ctctggattc tccctcagta gtagctactg catatgttgg      120

gtccgccagg ctccagggaa ggggctggag tggatcggat gcatttatgc tggtagtagt      180

ggtagcactt actacgcgag ctgggtgaat ggccgattca ctctctccag agacattgac      240

cagagcacag gttgcctaca actgaacagt ctgacagccg cggacacggc catgtattac      300

tgtgcgagag ccccctatag tagtggctgg gtcctctact ttaacttgtg gggcccaggc      360

accctggtca ttgtctcctc aggcggaggg ggctctggcg gcggaggatc tgggggaggg      420

ggcagcgccc aagtgctgac ccagactcca tcgcctgtgt ctgcagctgt gggaggcaca      480

gtcaccatca gttgccagtc cagtgagagt gtttatggta acagccgctt agcctggtat      540

cagcagaaac cagggcagtc tcccaagctc ctgatctatt atgcatccac tctggcatct      600

ggggtccctt cgcggttcaa aggcagtgga tctgggacac agttcactct caccattagc      660

gacctggagt gtgacgatgc tgcctcttac tactgtcaag gcggttatta tagtggtaat      720

cttgatgcgc ttgctttc                                                    738


<210>  69
<211>  762
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VL-linker-VH of DNP scFv (XC) coding sequence

<400>  69
gcccaagtgc tgacccagac tccatcgcct gtgtctgcag ctgtgggagg cacagtcacc       60

atcagttgcc agtccagtga gagtgtttat ggtaacagcc gcttagcctg gtatcagcag      120

aaaccagggc agtctcccaa gctcctgatc tattatgcat ccactctggc atctggggtc      180

ccttcgcggt tcaaaggcag tggatctggg acacagttca ctctcaccat tagcgacctg      240

gagtgtgacg atgctgcctc ttactactgt caaggcggtt attatagtgg taatcttgat      300

gcgcttgctt tcggcggagg gaccgaggtg gtggtcagag gtggcggagg gggctctggc      360

ggcggaggat ctgggggagg gggcagccag cagctggagc agtccggagg aggagccgaa      420

ggaggcctgg tcaagcctgg gggatccctg gaactctgct gcaaagcctc tggattctcc      480

ctcagtagta gctactgcat atgttgggtc cgccaggctc cagggaaggg gctggagtgg      540

atcggatgca tttatgctgg tagtagtggt agcacttact acgcgagctg ggtgaatggc      600

cgattcactc tctccagaga cattgaccag agcacaggtt gcctacaact gaacagtctg      660

acagccgcgg acacggccat gtattactgt gcgagagccc cctatagtag tggctgggtc      720

ctctacttta acttgtgggg cccaggcacc ctggtcattg tc                         762


<210>  70
<211>  789
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VH-linker-VL of DNP scFv (Ab-3) coding sequence

<400>  70
ccaggtgcca catttgccca agtgctgacc cagactccat cgcctgtgtc tgcagctgtg       60

ggaggcacag tcaccatcag ttgccagtcc agtgagagtg tttatggtaa cagccgctta      120

gcctggtatc agcagaaacc agggcagtct cccaagctcc tgatctatta tgcatccact      180

ctggcatctg gggtcccttc gcggttcaaa ggcagtggat ctgggacaca gttcactctc      240

accattagcg acctggagtg tgacgatgct gcctcttact actgtcaagg cggttattat      300

agtggtaatc ttgatgcgct tgctttcggc ggagggaccg aggtggtggt cagaggtggc      360

ggagggggct ctggcggcgg aggatctggg ggagggggca gccagtgtca gcagctggag      420

cagtccggag gaggagccga aggaggcctg gtcaagcctg ggggatccct ggaactctgc      480

tgcaaagcct ctggattctc cctcagtagt agctactgca tatgttgggt ccgccaggct      540

ccagggaagg ggctggagtg gatcggatgc atttatgctg gtagtagtgg tagcacttac      600

tacgcgagct gggtgaatgg ccgattcact ctctccagag acattgacca gagcacaggt      660

tgcctacaac tgaacagtct gacagccgcg gacacggcca tgtattactg tgcgagagcc      720

ccctatagta gtggctgggt cctctacttt aacttgtggg gcccaggcac cctggtcatt      780

gtctcctca                                                              789


<210>  71
<211>  789
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNP scFv VH-linker-VL of DNP scFv (Ab-3)

<400>  71
cagtgtcagc agctggagca gtccggagga ggagccgaag gaggcctggt caagcctggg       60

ggatccctgg aactctgctg caaagcctct ggattctccc tcagtagtag ctactgcata      120

tgttgggtcc gccaggctcc agggaagggg ctggagtgga tcggatgcat ttatgctggt      180

agtagtggta gcacttacta cgcgagctgg gtgaatggcc gattcactct ctccagagac      240

attgaccaga gcacaggttg cctacaactg aacagtctga cagccgcgga cacggccatg      300

tattactgtg cgagagcccc ctatagtagt ggctgggtcc tctactttaa cttgtggggc      360

ccaggcaccc tggtcattgt ctcctcaggc ggagggggct ctggcggcgg aggatctggg      420

ggagggggca gcccaggtgc cacatttgcc caagtgctga cccagactcc atcgcctgtg      480

tctgcagctg tgggaggcac agtcaccatc agttgccagt ccagtgagag tgtttatggt      540

aacagccgct tagcctggta tcagcagaaa ccagggcagt ctcccaagct cctgatctat      600

tatgcatcca ctctggcatc tggggtccct tcgcggttca aaggcagtgg atctgggaca      660

cagttcactc tcaccattag cgacctggag tgtgacgatg ctgcctctta ctactgtcaa      720

ggcggttatt atagtggtaa tcttgatgcg cttgctttcg gcggagggac cgaggtggtg      780

gtcagaggt                                                              789


<210>  72
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Glycine-serine linker

<400>  72

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  73
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Glycine-serine linker

<400>  73

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser 
            20  


<210>  74
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Glycine-serine linker

<400>  74

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10  


<210>  75
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Glycine-serine linker

<400>  75

Gly Gly Gly Gly Ser 
1               5   


<210>  76
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Coding sequence of (G_4S)_3 linker

<400>  76
ggcggaggcg gctccggagg aggagggtct ggaggcgggg gaagt                       45


<210>  77
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Coding sequence of (G_4S)_4 linker

<400>  77
ggcgggggag gctccggggg aggcggctcc ggcggcgggg gaagtggcgg gggaggatcc       60


<210>  78
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Short Spacer (IgG4 (short hinge))

<400>  78

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
1               5                   10          


<210>  79
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Short Spacer (IgG4 (short hinge)) Coding Sequence

<400>  79
gagtctaagt acggtccacc ttgccccccc tgccct                                 36


<210>  80
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Short Spacer 2, Alternative IgG4 linker

<400>  80

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys 
1               5                   10      


<210>  81
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Medium Spacer

<400>  81

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Gly Gln Pro Arg 
1               5                   10                  15      


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
            20                  25                  30          


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
        35                  40                  45              


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
    50                  55                  60                  


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
65                  70                  75                  80  


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
                85                  90                  95      


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
            100                 105                 110         


Leu Ser Leu Ser Leu Gly Lys 
        115                 


<210>  82
<211>  229
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Long spacer with single (L235D) mutation

<400>  82

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe 
1               5                   10                  15      


Asp Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            100                 105                 110         


Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
            180                 185                 190         


Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 


Leu Ser Leu Gly Lys 
225                 


<210>  83
<211>  229
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Long spacer with double (L235D, N297Q) mutations

<400>  83

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe 
1               5                   10                  15      


Asp Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Gln Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            100                 105                 110         


Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
            180                 185                 190         


Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 


Leu Ser Leu Gly Lys 
225                 


<210>  84

<400>  84
000

<210>  85
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P2A

<400>  85

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  86
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P2A Coding Sequence

<400>  86
ggaagtggtg caaccaactt ctctcttttg aaacaggctg gagatgtcga ggagaaccct       60

gggcct                                                                  66


<210>  87
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  T2A

<400>  87

Gly Gly Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu 
1               5                   10                  15      


Glu Asn Pro Gly Pro 
            20      


<210>  88
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T2A Coding Sequence

<400>  88
ggcggcggcg aagggcgagg aagtttgctt acctgcgggg atgtggagga aaaccccggt       60

cct                                                                     63


<210>  89
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E2A

<400>  89

Gly Ser Gly Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp 
1               5                   10                  15      


Val Glu Ser Asn Pro Gly Pro Pro 
            20                  


<210>  90
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  F2A

<400>  90

Gly Ser Gly Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala 
1               5                   10                  15      


Gly Asp Val Glu Ser Asn Pro Gly Pro 
            20                  25  


<210>  91
<211>  187
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Dihydrofolate reductase double mutant

<400>  91

Met Val Gly Ser Leu Asn Cys Ile Val Ala Val Ser Gln Asn Met Gly 
1               5                   10                  15      


Ile Gly Lys Asn Gly Asp Phe Pro Trp Pro Pro Leu Arg Asn Glu Ser 
            20                  25                  30          


Arg Tyr Phe Gln Arg Met Thr Thr Thr Ser Ser Val Glu Gly Lys Gln 
        35                  40                  45              


Asn Leu Val Ile Met Gly Lys Lys Thr Trp Phe Ser Ile Pro Glu Lys 
    50                  55                  60                  


Asn Arg Pro Leu Lys Gly Arg Ile Asn Leu Val Leu Ser Arg Glu Leu 
65                  70                  75                  80  


Lys Glu Pro Pro Gln Gly Ala His Phe Leu Ser Arg Ser Leu Asp Asp 
                85                  90                  95      


Ala Leu Lys Leu Thr Glu Gln Pro Glu Leu Ala Asn Lys Val Asp Met 
            100                 105                 110         


Val Trp Ile Val Gly Gly Ser Ser Val Tyr Lys Glu Ala Met Asn His 
        115                 120                 125             


Pro Gly His Leu Lys Leu Phe Val Thr Arg Ile Met Gln Asp Phe Glu 
    130                 135                 140                 


Ser Asp Thr Phe Phe Pro Glu Ile Asp Leu Glu Lys Tyr Lys Leu Leu 
145                 150                 155                 160 


Pro Glu Tyr Pro Gly Val Leu Ser Asp Val Gln Glu Glu Lys Gly Ile 
                165                 170                 175     


Lys Tyr Lys Phe Glu Val Tyr Glu Lys Asn Asp 
            180                 185         


<210>  92
<211>  561
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DHFRdm Coding Sequence

<400>  92
atggtaggtt ccctcaattg tatagtggct gtcagtcaaa acatgggtat aggcaaaaat       60

ggcgacttcc cgtggccgcc gctcaggaac gaatcaagat acttccaacg catgacgaca      120

actagctctg tcgaagggaa gcagaatctc gtcatcatgg gtaagaagac gtggttctct      180

ataccagaga aaaatcgccc gttgaaaggg aggataaacc tcgtgctttc acgcgaattg      240

aaggaacctc cgcaaggggc ccactttctg agtcgctcac tcgacgatgc gttgaaactg      300

actgaacagc ctgagttggc aaacaaagtc gacatggtgt ggatcgtggg aggaagcagt      360

gtttataaag aagcaatgaa ccaccccgga cacctgaagc ttttcgttac ccgaataatg      420

caggactttg aatcagatac attcttccct gaaattgacc ttgaaaaata taaacttctc      480

cctgaatacc ctggcgtgct gtccgacgtc caagaggaaa aaggtattaa gtacaagttt      540

gaggtatatg aaaaaaatga t                                                561


<210>  93
<211>  323
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Truncated CD19

<400>  93

Met Pro Pro Pro Arg Leu Leu Phe Phe Leu Leu Phe Leu Thr Pro Met 
1               5                   10                  15      


Glu Val Arg Pro Glu Glu Pro Leu Val Val Lys Val Glu Glu Gly Asp 
            20                  25                  30          


Asn Ala Val Leu Gln Cys Leu Lys Gly Thr Ser Asp Gly Pro Thr Gln 
        35                  40                  45              


Gln Leu Thr Trp Ser Arg Glu Ser Pro Leu Lys Pro Phe Leu Lys Leu 
    50                  55                  60                  


Ser Leu Gly Leu Pro Gly Leu Gly Ile His Met Arg Pro Leu Ala Ile 
65                  70                  75                  80  


Trp Leu Phe Ile Phe Asn Val Ser Gln Gln Met Gly Gly Phe Tyr Leu 
                85                  90                  95      


Cys Gln Pro Gly Pro Pro Ser Glu Lys Ala Trp Gln Pro Gly Trp Thr 
            100                 105                 110         


Val Asn Val Glu Gly Ser Gly Glu Leu Phe Arg Trp Asn Val Ser Asp 
        115                 120                 125             


Leu Gly Gly Leu Gly Cys Gly Leu Lys Asn Arg Ser Ser Glu Gly Pro 
    130                 135                 140                 


Ser Ser Pro Ser Gly Lys Leu Met Ser Pro Lys Leu Tyr Val Trp Ala 
145                 150                 155                 160 


Lys Asp Arg Pro Glu Ile Trp Glu Gly Glu Pro Pro Cys Val Pro Pro 
                165                 170                 175     


Arg Asp Ser Leu Asn Gln Ser Leu Ser Gln Asp Leu Thr Met Ala Pro 
            180                 185                 190         


Gly Ser Thr Leu Trp Leu Ser Cys Gly Val Pro Pro Asp Ser Val Ser 
        195                 200                 205             


Arg Gly Pro Leu Ser Trp Thr His Val His Pro Lys Gly Pro Lys Ser 
    210                 215                 220                 


Leu Leu Ser Leu Glu Leu Lys Asp Asp Arg Pro Ala Arg Asp Met Trp 
225                 230                 235                 240 


Val Met Glu Thr Gly Leu Leu Leu Pro Arg Ala Thr Ala Gln Asp Ala 
                245                 250                 255     


Gly Lys Tyr Tyr Cys His Arg Gly Asn Leu Thr Met Ser Phe His Leu 
            260                 265                 270         


Glu Ile Thr Ala Arg Pro Val Leu Trp His Trp Leu Leu Arg Thr Gly 
        275                 280                 285             


Gly Trp Lys Val Ser Ala Val Thr Leu Ala Tyr Leu Ile Phe Cys Leu 
    290                 295                 300                 


Cys Ser Leu Val Gly Ile Leu His Leu Gln Arg Ala Leu Val Leu Arg 
305                 310                 315                 320 


Arg Lys Arg 
            


<210>  94
<211>  969
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD19t Coding Sequence

<400>  94
atgcccccac ctcgcctgct cttttttctc ttgttcctca cgcccatgga agtccggccc       60

gaggagccac ttgtagtcaa ggtagaagag ggagacaacg cagtgctgca gtgccttaaa      120

ggcacaagtg atgggcccac ccaacaactg acatggagtc gcgagtcacc tcttaaaccc      180

ttcctcaaat tgagtctcgg cttgccgggc ttgggcattc atatgcgacc tctggctata      240

tggctgttca tttttaatgt ttcccagcag atggggggtt tttatttgtg ccagccaggc      300

ccgccaagcg aaaaagcctg gcagccagga tggacagtca atgtagaagg atctggtgag      360

ctttttaggt ggaacgtatc tgatttgggg ggacttggat gcggtttgaa gaaccgatct      420

tccgagggtc cgagctctcc tagtgggaaa ctgatgtctc ctaagctgta cgtttgggcg      480

aaagaccggc ccgagatttg ggagggcgag ccgccctgtg tgccgcctcg agactccctt      540

aatcagtccc tttcccagga cctgacgatg gcacctggga gtacgctgtg gcttagttgt      600

ggggtgccgc ctgatagtgt ctcccggggt cccctttcct ggacacacgt gcatccaaaa      660

ggcccgaagt ctcttttgtc tttggagctt aaagacgatc gccctgctcg cgatatgtgg      720

gtgatggaaa cgggattgct tctgccacgc gccaccgccc aggatgcggg gaagtattac      780

tgccatcgag ggaacctgac aatgagcttt catctggaaa tcactgccag accagtactg      840

tggcactggc ttttgcggac gggcggctgg aaagtgagcg cagtgacact ggcgtatctc      900

attttctgcc tttgtagtct cgtaggtatt ttgcacttgc aacgcgcgct tgtgcttcgc      960

agaaagcgg                                                              969


<210>  95
<211>  135
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  truncated Her2 (Her2)

<400>  95

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Cys His Pro Glu Cys Gln Pro Gln Asn Gly 
            20                  25                  30          


Ser Val Thr Cys Phe Gly Pro Glu Ala Asp Gln Cys Val Ala Cys Ala 
        35                  40                  45              


His Tyr Lys Asp Pro Pro Phe Cys Val Ala Arg Cys Pro Ser Gly Val 
    50                  55                  60                  


Lys Pro Asp Leu Ser Tyr Met Pro Ile Trp Lys Phe Pro Asp Glu Glu 
65                  70                  75                  80  


Gly Ala Cys Gln Pro Cys Pro Ile Asn Cys Thr His Ser Cys Val Asp 
                85                  90                  95      


Leu Asp Asp Lys Gly Cys Pro Ala Glu Gln Arg Ala Ser Pro Leu Thr 
            100                 105                 110         


Ser Ile Ile Ser Ala Val Val Gly Ile Leu Leu Val Val Val Leu Gly 
        115                 120                 125             


Val Val Phe Gly Ile Leu Ile 
    130                 135 


<210>  96
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Her2tG

<400>  96

Cys His Pro Glu Cys Gln Pro Gln Asn Gly Ser Val Thr Cys Phe Gly 
1               5                   10                  15      


Pro Glu Ala Asp Gln Cys Val Ala Cys Ala His Tyr Lys Asp Pro Pro 
            20                  25                  30          


Phe Cys Val Ala Arg Cys Pro Ser Gly Val Lys Pro Asp Leu Ser Tyr 
        35                  40                  45              


Met Pro Ile Trp Lys Phe Pro Asp Glu Glu Gly Ala Cys Gln Pro Cys 
    50                  55                  60                  


Pro Ile Asn Cys Thr His Ser Cys Val Asp Leu Asp Asp Lys Gly Cys 
65                  70                  75                  80  


Pro Ala Glu Gln Arg Ala Ser Pro Leu Thr Gly Gly Gly Ser Gly Gly 
                85                  90                  95      


Gly Ser Ile Ile Ser Ala Val Val Gly Ile Leu Leu Val Val Val Leu 
            100                 105                 110         


Gly Val Val Phe Gly Ile Leu Ile 
        115                 120 


<210>  97
<211>  335
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EGFRt

<400>  97

Arg Lys Val Cys Asn Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu 
1               5                   10                  15      


Ser Ile Asn Ala Thr Asn Ile Lys His Phe Lys Asn Cys Thr Ser Ile 
            20                  25                  30          


Ser Gly Asp Leu His Ile Leu Pro Val Ala Phe Arg Gly Asp Ser Phe 
        35                  40                  45              


Thr His Thr Pro Pro Leu Asp Pro Gln Glu Leu Asp Ile Leu Lys Thr 
    50                  55                  60                  


Val Lys Glu Ile Thr Gly Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn 
65                  70                  75                  80  


Arg Thr Asp Leu His Ala Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg 
                85                  90                  95      


Thr Lys Gln His Gly Gln Phe Ser Leu Ala Val Val Ser Leu Asn Ile 
            100                 105                 110         


Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu Ile Ser Asp Gly Asp Val 
        115                 120                 125             


Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr Ala Asn Thr Ile Asn Trp 
    130                 135                 140                 


Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys Thr Lys Ile Ile Ser Asn 
145                 150                 155                 160 


Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly Gln Val Cys His Ala Leu 
                165                 170                 175     


Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu Pro Arg Asp Cys Val Ser 
            180                 185                 190         


Cys Arg Asn Val Ser Arg Gly Arg Glu Cys Val Asp Lys Cys Asn Leu 
        195                 200                 205             


Leu Glu Gly Glu Pro Arg Glu Phe Val Glu Asn Ser Glu Cys Ile Gln 
    210                 215                 220                 


Cys His Pro Glu Cys Leu Pro Gln Ala Met Asn Ile Thr Cys Thr Gly 
225                 230                 235                 240 


Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala His Tyr Ile Asp Gly Pro 
                245                 250                 255     


His Cys Val Lys Thr Cys Pro Ala Gly Val Met Gly Glu Asn Asn Thr 
            260                 265                 270         


Leu Val Trp Lys Tyr Ala Asp Ala Gly His Val Cys His Leu Cys His 
        275                 280                 285             


Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro Gly Leu Glu Gly Cys Pro 
    290                 295                 300                 


Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala Thr Gly Met Val Gly Ala 
305                 310                 315                 320 


Leu Leu Leu Leu Leu Val Val Ala Leu Gly Ile Gly Leu Phe Met 
                325                 330                 335 


<210>  98
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GM-CSF Signal Peptide

<400>  98

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro 
            20          


<210>  99
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 signal peptide

<400>  99

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg 
            20  


<210>  100
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His tag

<400>  100

His His His His His His 
1               5       


<210>  101
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Flag tag

<400>  101

Asp Tyr Lys Asp Asp Asp Asp 
1               5           


<210>  102
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Flag tag

<400>  102

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  103
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Flag tag

<400>  103

Asp Tyr Lys Asp Asp Asp Lys 
1               5           


<210>  104
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Xpress tag

<400>  104

Asp Leu Tyr Asp Asp Asp Asp Lys 
1               5               


<210>  105
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Avi tag

<400>  105

Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu 
1               5                   10                  15  


<210>  106
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Calmodulin binding peptide (CBP) tag

<400>  106

Lys Arg Arg Trp Lys Lys Asn Phe Ile Ala Val Ser Ala Ala Asn Arg 
1               5                   10                  15      


Phe Lys Lys Ile Ser Ser Ser Gly Ala Leu 
            20                  25      


<210>  107
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polyglutamate tag

<400>  107

Glu Glu Glu Glu Glu Glu 
1               5       


<210>  108
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HA tag

<400>  108

Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210>  109
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HA tag

<400>  109

Tyr Ala Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210>  110
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HA tag

<400>  110

Tyr Asp Val Pro Asp Tyr Ala Ser Leu 
1               5                   


<210>  111
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Myc tag

<400>  111

Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
1               5                   10  


<210>  112
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Strep tag

<400>  112

Trp Arg His Pro Gln Phe Gly Gly 
1               5               


<210>  113
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  STREP(R) tag II

<400>  113

Trp Ser His Pro Gln Phe Glu Lys 
1               5               


<210>  114
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Softag 1

<400>  114

Ser Leu Ala Glu Leu Leu Asn Ala Gly Leu Gly Gly Ser 
1               5                   10              


<210>  115
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Softag 3

<400>  115

Thr Gln Asp Pro Ser Arg Val Gly 
1               5               


<210>  116
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V5 tag

<400>  116

Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr 
1               5                   10                  


<210>  117
<211>  399
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MND promoter

<400>  117
tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct       60

aggatcaagg ttaggaacag agagacagca gaatatgggc caaacaggat atctgtggta      120

agcagttcct gccccggctc agggccaaga acagttggaa cagcagaata tgggccaaac      180

aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag      240

atgcggtccc gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag      300

gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt      360

tcgcgcgctt ctgctccccg agctcaataa aagagccca                             399


<210>  118

<400>  118
000

<210>  119

<400>  119
000

<210>  120
<211>  255
<212>  PRT
<213>  Homo sapiens

<400>  120

Met Gly Asn Ser Cys Tyr Asn Ile Val Ala Thr Leu Leu Leu Val Leu 
1               5                   10                  15      


Asn Phe Glu Arg Thr Arg Ser Leu Gln Asp Pro Cys Ser Asn Cys Pro 
            20                  25                  30          


Ala Gly Thr Phe Cys Asp Asn Asn Arg Asn Gln Ile Cys Ser Pro Cys 
        35                  40                  45              


Pro Pro Asn Ser Phe Ser Ser Ala Gly Gly Gln Arg Thr Cys Asp Ile 
    50                  55                  60                  


Cys Arg Gln Cys Lys Gly Val Phe Arg Thr Arg Lys Glu Cys Ser Ser 
65                  70                  75                  80  


Thr Ser Asn Ala Glu Cys Asp Cys Thr Pro Gly Phe His Cys Leu Gly 
                85                  90                  95      


Ala Gly Cys Ser Met Cys Glu Gln Asp Cys Lys Gln Gly Gln Glu Leu 
            100                 105                 110         


Thr Lys Lys Gly Cys Lys Asp Cys Cys Phe Gly Thr Phe Asn Asp Gln 
        115                 120                 125             


Lys Arg Gly Ile Cys Arg Pro Trp Thr Asn Cys Ser Leu Asp Gly Lys 
    130                 135                 140                 


Ser Val Leu Val Asn Gly Thr Lys Glu Arg Asp Val Val Cys Gly Pro 
145                 150                 155                 160 


Ser Pro Ala Asp Leu Ser Pro Gly Ala Ser Ser Val Thr Pro Pro Ala 
                165                 170                 175     


Pro Ala Arg Glu Pro Gly His Ser Pro Gln Ile Ile Ser Phe Phe Leu 
            180                 185                 190         


Ala Leu Thr Ser Thr Ala Leu Leu Phe Leu Leu Phe Phe Leu Thr Leu 
        195                 200                 205             


Arg Phe Ser Val Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe 
    210                 215                 220                 


Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly 
225                 230                 235                 240 


Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
                245                 250                 255 


<210>  121
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3-zeta ICD

<400>  121

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  122

<400>  122
000

<210>  123

<400>  123
000

<210>  124
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD3-zeta ICD Coding Sequence

<400>  124
agagtgaagt tcagcagatc cgccgacgct cctgcctatc agcagggaca gaaccagctg       60

tacaacgagc tgaacctggg gagaagagaa gagtacgacg tgctggataa gcggagaggc      120

agggatcctg agatgggcgg caagcccaga cggaagaatc ctcaagaggg cctgtataat      180

gagctgcaga aagacaagat ggccgaggcc tacagcgaga tcggaatgaa gggcgagcgc      240

agaagaggca agggacacga tggactgtac cagggactga gcaccgccac caaggatacc      300

tatgacgcac tgcacatgca ggccctgcca cctcgt                                336


<210>  125

<400>  125
000

<210>  126
<211>  69
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8-alpha Hinge/Transmembrane domain

<400>  126

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile 
        35                  40                  45              


Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val 
    50                  55                  60                  


Ile Thr Leu Tyr Cys 
65                  


<210>  127
<211>  75
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Alternative CD8 hinge/Transmembrane domain

<400>  127

Glu Ser Lys Tyr Gly Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg 
1               5                   10                  15      


Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg 
            20                  25                  30          


Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly 
        35                  40                  45              


Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr 
    50                  55                  60                  


Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr 
65                  70                  75  


<210>  128
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Alternative CD8 hinge/Transmembrane domain (residues 183-203 of 
       UniProt ID P01732-1)

<400>  128

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr 
            20      


<210>  129
<211>  48
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Alternative Human CD8 alpha chain hinge (residues 135-182 of 
       UniProt ID P01732-1)

<400>  129

Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro 
1               5                   10                  15      


Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro 
            20                  25                  30          


Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        35                  40                  45              


<210>  130
<211>  154
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4-1BB/CD3z intracellular signaling domain

<400>  130

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg 
        35                  40                  45              


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
    50                  55                  60                  


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
65                  70                  75                  80  


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
                85                  90                  95      


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
            100                 105                 110         


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
        115                 120                 125             


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
    130                 135                 140                 


Ala Leu His Met Gln Ala Leu Pro Pro Arg 
145                 150                 


<210>  131
<211>  462
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Coding sequence of 4-1BB/CD3z intracellular signaling domains

<400>  131
aaaaggggac gcaaaaaact cctttacatt ttcaaacagc cctttatgcg acctgtacaa       60

acaactcagg aagaggatgg ctgcagctgt aggtttcccg aggaagaaga gggtggctgc      120

gagctcagag tgaagttcag cagatccgcc gacgctcctg cctatcagca gggacagaac      180

cagctgtaca acgagctgaa cctggggaga agagaagagt acgacgtgct ggataagcgg      240

agaggcaggg atcctgagat gggcggcaag cccagacgga agaatcctca agagggcctg      300

tataatgagc tgcagaaaga caagatggcc gaggcctaca gcgagatcgg aatgaagggc      360

gagcgcagaa gaggcaaggg acacgatgga ctgtaccagg gactgagcac cgccaccaag      420

gatacctatg acgcactgca catgcaggcc ctgccacctc gt                         462


<210>  132
<211>  462
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Alternative Coding sequence of 4-1BB/CD3z intracellular signaling
       domains

<400>  132
aaacggggca gaaagaaact cctgtatata ttcaaacaac catttatgag accagtacaa       60

actactcaag aggaagatgg ctgtagctgc cgatttccag aagaagaaga aggaggatgt      120

gaactgcggg tgaagttcag cagaagcgcc gacgcccctg cctaccagca gggccagaat      180

cagctgtaca acgagctgaa cctgggcaga agggaagagt acgacgtcct ggataagcgg      240

agaggccggg accctgagat gggcggcaag cctcggcgga agaaccccca ggaaggcctg      300

tataacgaac tgcagaaaga caagatggcc gaggcctaca gcgagatcgg catgaagggc      360

gagcggaggc ggggcaaggg ccacgacggc ctgtatcagg gcctgtccac cgccaccaag      420

gatacctacg acgccctgca catgcaggcc ctgcccccaa gg                         462


<210>  133
<211>  942
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EBD (L384M, M421G, G521R) Pairs with estrogen analog CMP8 Coding 
       Sequence

<400>  133
tctgctggcg atatgagagc cgctaacctg tggccttctc cactgatgat caagcggagc       60

aagaagaact ctctggccct gtctctgacc gccgaccaga tggtttctgc tctgctggat      120

gccgagcctc ctatcctgta ctccgagtac gaccccacca gaccttttag cgaggccagc      180

atgatgggcc tgctgaccaa tctggccgac agagaactgg tgcacatgat caactgggcc      240

aagcgcgtgc ccggctttgt cgatctgaca ctgcacgacc aagtgcatct gctggaatgc      300

gcctggatgg aaatcctgat gatcggcctc gtgtggcgga gcatggaaca ccctggaaaa      360

ctgctgttcg cccctaacct gctgctcgac agaaaccagg gcaaatgcgt ggaaggcggc      420

gtggaaatct tcgatatgct gctggccacc tccagccggt tccggatgat gaatctccag      480

ggcgaagagt tcgtgtgcct gaagtccatc atcctgctga atagcggcgt gtacacattc      540

ctgagcagca ccctgaaatc cctggaagag aaggaccaca tccaccgggt gctcgacaag      600

atcaccgaca cactgatcca cctgatggcc aaggccggac tgactctgca gcagcaacat      660

cagagactgg cccagctgct gctcatcctg agccacatca gacacatgag caacaagcgg      720

atggaacatc tgtactccat gaagtgcaag aacgtggtgc ccctgtacga cctgctcctg      780

gaaatgctgg acgcccacag actgcacgcc cctacatcta gaggtggcgc ctctgtggaa      840

gagacagacc agtctcacct ggccacagcc ggcagcacaa gctctcattc cctgcagaag      900

tactacatca ccggcgaggc cgagggcttt ccagccacag tt                         942


<210>  134
<211>  942
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G400V/M543/L544A ER(T2) Coding Sequence

<400>  134
tctgctggcg atatgagagc cgccaacctg tggcccagcc ccctgatgat caagcggagc       60

aagaagaaca gcctggccct gagcctgacc gccgatcaga tggtgtccgc tctgctggac      120

gccgagcccc ctatcctgta cagcgagtac gaccccacca gacccttcag cgaggccagc      180

atgatgggcc tgctgaccaa cctggccgac cgggagctgg tgcacatgat caactgggcc      240

aagcgggtgc ccggcttcgt ggacctgacc ctgcacgacc aggtccacct gctggaatgt      300

gcctggctgg aaatcctgat gatcggcctc gtgtggagaa gcatggaaca ccccgtgaag      360

ctgctgttcg cccccaacct gctcctggac cggaaccagg gaaagtgcgt ggagggcatg      420

gtggagatct tcgacatgct gctggccacc tccagccggt tccggatgat gaacctgcag      480

ggcgaggaat tcgtgtgcct gaagtccatc atcctgctga acagcggcgt gtacaccttc      540

ctgtcatcca ccctgaagtc cctggaagag aaggaccaca tccaccgggt gctggacaag      600

atcaccgaca ccctgatcca cctgatggcc aaggctggcc tgacactcca gcagcagcac      660

cagagactgg cccagctgct gctgatcctg agccacatcc ggcacatgag caacaaggga      720

atggaacacc tgtacagcat gaagtgcaag aacgtggtgc ccctgtacga cctgctgctc      780

gaggctgccg atgcccacag actgcacgcc cctacaagca gaggcggagc cagcgtggag      840

gaaaccgacc agtctcacct ggccaccgcc ggcagcacaa gcagccacag cctgcagaag      900

tactacatca ccggcgaggc cgagggattc cctgccaccg tg                         942


<210>  135
<211>  55
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3-epsilon ICD

<400>  135

Lys Asn Arg Lys Ala Lys Ala Lys Pro Val Thr Arg Gly Ala Gly Ala 
1               5                   10                  15      


Gly Gly Arg Gln Arg Gly Gln Asn Lys Glu Arg Pro Pro Pro Val Pro 
            20                  25                  30          


Asn Pro Asp Tyr Glu Pro Ile Arg Lys Gly Gln Arg Asp Leu Tyr Ser 
        35                  40                  45              


Gly Leu Asn Gln Arg Arg Ile 
    50                  55  


<210>  136
<211>  770
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA-STAT3: Mutations at A662C and N664C for constitutive 
       activation

<400>  136

Met Ala Gln Trp Asn Gln Leu Gln Gln Leu Asp Thr Arg Tyr Leu Glu 
1               5                   10                  15      


Gln Leu His Gln Leu Tyr Ser Asp Ser Phe Pro Met Glu Leu Arg Gln 
            20                  25                  30          


Phe Leu Ala Pro Trp Ile Glu Ser Gln Asp Trp Ala Tyr Ala Ala Ser 
        35                  40                  45              


Lys Glu Ser His Ala Thr Leu Val Phe His Asn Leu Leu Gly Glu Ile 
    50                  55                  60                  


Asp Gln Gln Tyr Ser Arg Phe Leu Gln Glu Ser Asn Val Leu Tyr Gln 
65                  70                  75                  80  


His Asn Leu Arg Arg Ile Lys Gln Phe Leu Gln Ser Arg Tyr Leu Glu 
                85                  90                  95      


Lys Pro Met Glu Ile Ala Arg Ile Val Ala Arg Cys Leu Trp Glu Glu 
            100                 105                 110         


Ser Arg Leu Leu Gln Thr Ala Ala Thr Ala Ala Gln Gln Gly Gly Gln 
        115                 120                 125             


Ala Asn His Pro Thr Ala Ala Val Val Thr Glu Lys Gln Gln Met Leu 
    130                 135                 140                 


Glu Gln His Leu Gln Asp Val Arg Lys Arg Val Gln Asp Leu Glu Gln 
145                 150                 155                 160 


Lys Met Lys Val Val Glu Asn Leu Gln Asp Asp Phe Asp Phe Asn Tyr 
                165                 170                 175     


Lys Thr Leu Lys Ser Gln Gly Asp Met Gln Asp Leu Asn Gly Asn Asn 
            180                 185                 190         


Gln Ser Val Thr Arg Gln Lys Met Gln Gln Leu Glu Gln Met Leu Thr 
        195                 200                 205             


Ala Leu Asp Gln Met Arg Arg Ser Ile Val Ser Glu Leu Ala Gly Leu 
    210                 215                 220                 


Leu Ser Ala Met Glu Tyr Val Gln Lys Thr Leu Thr Asp Glu Glu Leu 
225                 230                 235                 240 


Ala Asp Trp Lys Arg Arg Gln Gln Ile Ala Cys Ile Gly Gly Pro Pro 
                245                 250                 255     


Asn Ile Cys Leu Asp Arg Leu Glu Asn Trp Ile Thr Ser Leu Ala Glu 
            260                 265                 270         


Ser Gln Leu Gln Thr Arg Gln Gln Ile Lys Lys Leu Glu Glu Leu Gln 
        275                 280                 285             


Gln Lys Val Ser Tyr Lys Gly Asp Pro Ile Val Gln His Arg Pro Met 
    290                 295                 300                 


Leu Glu Glu Arg Ile Val Glu Leu Phe Arg Asn Leu Met Lys Ser Ala 
305                 310                 315                 320 


Phe Val Val Glu Arg Gln Pro Cys Met Pro Met His Pro Asp Arg Pro 
                325                 330                 335     


Leu Val Ile Lys Thr Gly Val Gln Phe Thr Thr Lys Val Arg Leu Leu 
            340                 345                 350         


Val Lys Phe Pro Glu Leu Asn Tyr Gln Leu Lys Ile Lys Val Cys Ile 
        355                 360                 365             


Asp Lys Asp Ser Gly Asp Val Ala Ala Leu Arg Gly Ser Arg Lys Phe 
    370                 375                 380                 


Asn Ile Leu Gly Thr Asn Thr Lys Val Met Asn Met Glu Glu Ser Asn 
385                 390                 395                 400 


Asn Gly Ser Leu Ser Ala Glu Phe Lys His Leu Thr Leu Arg Glu Gln 
                405                 410                 415     


Arg Cys Gly Asn Gly Gly Arg Ala Asn Cys Asp Ala Ser Leu Ile Val 
            420                 425                 430         


Thr Glu Glu Leu His Leu Ile Thr Phe Glu Thr Glu Val Tyr His Gln 
        435                 440                 445             


Gly Leu Lys Ile Asp Leu Glu Thr His Ser Leu Pro Val Val Val Ile 
    450                 455                 460                 


Ser Asn Ile Cys Gln Met Pro Asn Ala Trp Ala Ser Ile Leu Trp Tyr 
465                 470                 475                 480 


Asn Met Leu Thr Asn Asn Pro Lys Asn Val Asn Phe Phe Thr Lys Pro 
                485                 490                 495     


Pro Ile Gly Thr Trp Asp Gln Val Ala Glu Val Leu Ser Trp Gln Phe 
            500                 505                 510         


Ser Ser Thr Thr Lys Arg Gly Leu Ser Ile Glu Gln Leu Thr Thr Leu 
        515                 520                 525             


Ala Glu Lys Leu Leu Gly Pro Gly Val Asn Tyr Ser Gly Cys Gln Ile 
    530                 535                 540                 


Thr Trp Ala Lys Phe Cys Lys Glu Asn Met Ala Gly Lys Gly Phe Ser 
545                 550                 555                 560 


Phe Trp Val Trp Leu Asp Asn Ile Ile Asp Leu Val Lys Lys Tyr Ile 
                565                 570                 575     


Leu Ala Leu Trp Asn Glu Gly Tyr Ile Met Gly Phe Ile Ser Lys Glu 
            580                 585                 590         


Arg Glu Arg Ala Ile Leu Ser Thr Lys Pro Pro Gly Thr Phe Leu Leu 
        595                 600                 605             


Arg Phe Ser Glu Ser Ser Lys Glu Gly Gly Val Thr Phe Thr Trp Val 
    610                 615                 620                 


Glu Lys Asp Ile Ser Gly Lys Thr Gln Ile Gln Ser Val Glu Pro Tyr 
625                 630                 635                 640 


Thr Lys Gln Gln Leu Asn Asn Met Ser Phe Ala Glu Ile Ile Met Gly 
                645                 650                 655     


Tyr Lys Ile Met Asp Cys Thr Cys Ile Leu Leu Ser Pro Leu Val Tyr 
            660                 665                 670         


Leu Tyr Pro Asp Ile Pro Lys Glu Glu Ala Phe Gly Lys Tyr Cys Arg 
        675                 680                 685             


Pro Glu Ser Gln Glu His Pro Glu Ala Asp Pro Gly Ser Ala Ala Pro 
    690                 695                 700                 


Tyr Leu Lys Thr Lys Phe Ile Cys Val Thr Pro Thr Thr Cys Ser Asn 
705                 710                 715                 720 


Thr Ile Asp Leu Pro Met Ser Pro Arg Thr Leu Asp Ser Leu Met Gln 
                725                 730                 735     


Phe Gly Asn Asn Gly Glu Gly Ala Glu Pro Ser Ala Gly Gly Gln Phe 
            740                 745                 750         


Glu Ser Leu Thr Phe Asp Met Glu Leu Thr Ser Glu Cys Ala Thr Ser 
        755                 760                 765             


Pro Met 
    770 


<210>  137
<211>  2310
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CA-STAT3: Mutations at A662C and N664C for constitutive 
       activation Coding Sequence

<400>  137
atggctcagt ggaaccagct gcaacagctg gacaccagat acctcgagca gctccaccag       60

ctgtactctg acagcttccc aatggaactg aggcagtttc tggctccctg gattgagagc      120

caggattggg cctatgccgc ctccaaagaa tctcacgcca cactcgtgtt ccacaacctg      180

ctgggagaga tcgaccagca gtacagccgg ttcctgcaag agtccaacgt gctgtaccag      240

cacaacctga gaagaatcaa gcagttcctg cagagcagat acctggaaaa gcccatggaa      300

atcgccagaa ttgtggcccg gtgtctgtgg gaagaaagca gactgctgca aacagccgcc      360

actgctgcac agcaaggcgg acaggctaat catcctacag ccgccgtggt caccgagaaa      420

caacagatgc tggaacagca tctgcaggac gtgcggaaga gagtgcagga cctggaacag      480

aaaatgaagg tggtggaaaa cctgcaggat gacttcgact tcaactacaa gaccctgaaa      540

agccagggcg acatgcagga tctgaatggc aacaaccagt ccgtgaccag gcagaaaatg      600

cagcagctcg agcagatgct cacagccctg gatcagatgc ggagatccat tgtgtctgag      660

ctggccggac tgctgtccgc catggaatac gtgcagaaaa ctctgaccga tgaggaactg      720

gctgactgga agcggagaca gcagattgcc tgtatcggcg gacctcctaa catctgcctg      780

gaccggctgg aaaactggat cacctctctg gccgaatctc agctgcagac cagacagcaa      840

atcaagaagc tggaagaact ccagcaaaag gtgtcctaca agggcgaccc catcgtgcag      900

catagaccta tgctcgagga acggatcgtg gaactgttca gaaacctgat gaagtccgcc      960

ttcgtggtgg aacggcagcc ttgtatgcct atgcaccccg atagacccct ggtcatcaag     1020

accggcgtgc agtttaccac caaagtccgg ctgctggtca agttccccga gctgaactac     1080

cagctgaaaa tcaaagtgtg catcgacaag gactccggcg acgtggcagc tctgagaggc     1140

agcagaaagt tcaatatcct gggcaccaac accaaagtga tgaacatgga agagagcaac     1200

aacggctccc tgagcgccga gtttaaacac ctgacactga gagaacagag atgcggcaac     1260

ggcggcagag ccaattgtga tgccagcctg atcgtgacag aggaactgca cctgatcacc     1320

ttcgagacag aggtgtacca tcagggcctg aagattgacc tggaaaccca ctctctgccc     1380

gtggtcgtga tcagcaacat ctgtcagatg cccaacgcct gggccagcat cctgtggtac     1440

aacatgctga caaacaaccc caagaacgtc aacttcttca ccaagcctcc aatcggcacc     1500

tgggatcaag tggccgaagt gctgtcttgg cagttcagca gcaccaccaa gaggggcctg     1560

agcattgagc agctgaccac actggcagag aaactgcttg gccctggcgt gaactactcc     1620

ggctgtcaga tcacatgggc caagttctgt aaagaaaaca tggccggcaa gggcttcagc     1680

ttctgggtct ggctggataa catcatcgac ctcgtcaaga agtacattct cgccctgtgg     1740

aacgagggct atatcatggg cttcatctct aaagagagag agcgggccat cctgagcaca     1800

aagcctccag gcacttttct gctgaggttc tccgagtcca gcaaagaagg cggcgtcacc     1860

tttacctggg tcgagaagga tatcagcggc aagacccaga tccagagcgt ggaaccttac     1920

acaaaacagc agctgaacaa catgagcttc gccgagatca tcatggggta caagatcatg     1980

gactgtacct gcatcctgct gagccctctg gtgtatctgt accctgacat ccccaaagag     2040

gaagccttcg gcaagtactg cagacccgag tctcaagagc accccgaagc cgatcctgga     2100

tctgccgctc cttacctgaa aactaagttc atctgcgtga cccctaccac ctgtagcaac     2160

accatcgatc tgcctatgag ccccagaaca ctggactccc tgatgcagtt cggaaacaat     2220

ggcgagggcg cagaacctag tgccggcgga caatttgagt ccctgacctt cgatatggaa     2280

ctcaccagcg agtgcgccac ctctcctatg                                      2310


<210>  138
<211>  794
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  caSTAT5a: Mutations at H298R and S710F for constitutive 
       activation

<400>  138

Met Ala Gly Trp Ile Gln Ala Gln Gln Leu Gln Gly Asp Ala Leu Arg 
1               5                   10                  15      


Gln Met Gln Val Leu Tyr Gly Gln His Phe Pro Ile Glu Val Arg His 
            20                  25                  30          


Tyr Leu Ala Gln Trp Ile Glu Ser Gln Pro Trp Asp Ala Ile Asp Leu 
        35                  40                  45              


Asp Asn Pro Gln Asp Arg Ala Gln Ala Thr Gln Leu Leu Glu Gly Leu 
    50                  55                  60                  


Val Gln Glu Leu Gln Lys Lys Ala Glu His Gln Val Gly Glu Asp Gly 
65                  70                  75                  80  


Phe Leu Leu Lys Ile Lys Leu Gly His Tyr Ala Thr Gln Leu Gln Lys 
                85                  90                  95      


Thr Tyr Asp Arg Cys Pro Leu Glu Leu Val Arg Cys Ile Arg His Ile 
            100                 105                 110         


Leu Tyr Asn Glu Gln Arg Leu Val Arg Glu Ala Asn Asn Cys Ser Ser 
        115                 120                 125             


Pro Ala Gly Ile Leu Val Asp Ala Met Ser Gln Lys His Leu Gln Ile 
    130                 135                 140                 


Asn Gln Thr Phe Glu Glu Leu Arg Leu Val Thr Gln Asp Thr Glu Asn 
145                 150                 155                 160 


Glu Leu Lys Lys Leu Gln Gln Thr Gln Glu Tyr Phe Ile Ile Gln Tyr 
                165                 170                 175     


Gln Glu Ser Leu Arg Ile Gln Ala Gln Phe Ala Gln Leu Ala Gln Leu 
            180                 185                 190         


Ser Pro Gln Glu Arg Leu Ser Arg Glu Thr Ala Leu Gln Gln Lys Gln 
        195                 200                 205             


Val Ser Leu Glu Ala Trp Leu Gln Arg Glu Ala Gln Thr Leu Gln Gln 
    210                 215                 220                 


Tyr Arg Val Glu Leu Ala Glu Lys His Gln Lys Thr Leu Gln Leu Leu 
225                 230                 235                 240 


Arg Lys Gln Gln Thr Ile Ile Leu Asp Asp Glu Leu Ile Gln Trp Lys 
                245                 250                 255     


Arg Arg Gln Gln Leu Ala Gly Asn Gly Gly Pro Pro Glu Gly Ser Leu 
            260                 265                 270         


Asp Val Leu Gln Ser Trp Cys Glu Lys Leu Ala Glu Ile Ile Trp Gln 
        275                 280                 285             


Asn Arg Gln Gln Ile Arg Arg Ala Glu Arg Leu Cys Gln Gln Leu Pro 
    290                 295                 300                 


Ile Pro Gly Pro Val Glu Glu Met Leu Ala Glu Val Asn Ala Thr Ile 
305                 310                 315                 320 


Thr Asp Ile Ile Ser Ala Leu Val Thr Ser Thr Phe Ile Ile Glu Lys 
                325                 330                 335     


Gln Pro Pro Gln Val Leu Lys Thr Gln Thr Lys Phe Ala Ala Thr Val 
            340                 345                 350         


Arg Leu Leu Val Gly Gly Lys Leu Asn Val His Met Asn Pro Pro Gln 
        355                 360                 365             


Val Lys Ala Thr Ile Ile Ser Glu Gln Gln Ala Lys Ser Leu Leu Lys 
    370                 375                 380                 


Asn Glu Asn Thr Arg Asn Glu Cys Ser Gly Glu Ile Leu Asn Asn Cys 
385                 390                 395                 400 


Cys Val Met Glu Tyr His Gln Ala Thr Gly Thr Leu Ser Ala His Phe 
                405                 410                 415     


Arg Asn Met Ser Leu Lys Arg Ile Lys Arg Ala Asp Arg Arg Gly Ala 
            420                 425                 430         


Glu Ser Val Thr Glu Glu Lys Phe Thr Val Leu Phe Glu Ser Gln Phe 
        435                 440                 445             


Ser Val Gly Ser Asn Glu Leu Val Phe Gln Val Lys Thr Leu Ser Leu 
    450                 455                 460                 


Pro Val Val Val Ile Val His Gly Ser Gln Asp His Asn Ala Thr Ala 
465                 470                 475                 480 


Thr Val Leu Trp Asp Asn Ala Phe Ala Glu Pro Gly Arg Val Pro Phe 
                485                 490                 495     


Ala Val Pro Asp Lys Val Leu Trp Pro Gln Leu Cys Glu Ala Leu Asn 
            500                 505                 510         


Met Lys Phe Lys Ala Glu Val Gln Ser Asn Arg Gly Leu Thr Lys Glu 
        515                 520                 525             


Asn Leu Val Phe Leu Ala Gln Lys Leu Phe Asn Asn Ser Ser Ser His 
    530                 535                 540                 


Leu Glu Asp Tyr Ser Gly Leu Ser Val Ser Trp Ser Gln Phe Asn Arg 
545                 550                 555                 560 


Glu Asn Leu Pro Gly Trp Asn Tyr Thr Phe Trp Gln Trp Phe Asp Gly 
                565                 570                 575     


Val Met Glu Val Leu Lys Lys His His Lys Pro His Trp Asn Asp Gly 
            580                 585                 590         


Ala Ile Leu Gly Phe Val Asn Lys Gln Gln Ala His Asp Leu Leu Ile 
        595                 600                 605             


Asn Lys Pro Asp Gly Thr Phe Leu Leu Arg Phe Ser Asp Ser Glu Ile 
    610                 615                 620                 


Gly Gly Ile Thr Ile Ala Trp Lys Phe Asp Ser Pro Glu Arg Asn Leu 
625                 630                 635                 640 


Trp Asn Leu Lys Pro Phe Thr Thr Arg Asp Phe Ser Ile Arg Ser Leu 
                645                 650                 655     


Ala Asp Arg Leu Gly Asp Leu Ser Tyr Leu Ile Tyr Val Phe Pro Asp 
            660                 665                 670         


Arg Pro Lys Asp Glu Val Phe Ser Lys Tyr Tyr Thr Pro Val Leu Ala 
        675                 680                 685             


Lys Ala Val Asp Gly Tyr Val Lys Pro Gln Ile Lys Gln Val Val Pro 
    690                 695                 700                 


Glu Phe Val Asn Ala Phe Ala Asp Ala Gly Gly Ser Ser Ala Thr Tyr 
705                 710                 715                 720 


Met Asp Gln Ala Pro Ser Pro Ala Val Cys Pro Gln Ala Pro Tyr Asn 
                725                 730                 735     


Met Tyr Pro Gln Asn Pro Asp His Val Leu Asp Gln Asp Gly Glu Phe 
            740                 745                 750         


Asp Leu Asp Glu Thr Met Asp Val Ala Arg His Val Glu Glu Leu Leu 
        755                 760                 765             


Arg Arg Pro Met Asp Ser Leu Asp Ser Arg Leu Ser Pro Pro Ala Gly 
    770                 775                 780                 


Leu Phe Thr Ser Ala Arg Gly Ser Leu Ser 
785                 790                 


<210>  139
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3-delta ICD

<400>  139

Gly His Glu Thr Gly Arg Leu Ser Gly Ala Ala Asp Thr Gln Ala Leu 
1               5                   10                  15      


Leu Arg Asn Asp Gln Val Tyr Gln Pro Leu Arg Asp Arg Asp Asp Ala 
            20                  25                  30          


Gln Tyr Ser His Leu Gly Gly Asn Trp Ala Arg Asn Lys 
        35                  40                  45  


<210>  140
<211>  2382
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  caSTAT5a: Mutations at H298R and S710F for constitutive 
       activation Coding Sequence

<400>  140
atggcgggct ggatccaggc ccagcagctg cagggagacg cgctgcgcca gatgcaggtg       60

ctgtacggcc agcacttccc catcgaggtc cggcactact tggcccagtg gattgagagc      120

cagccatggg atgccattga cttggacaat ccccaggaca gagcccaagc cacccagctc      180

ctggagggcc tggtgcagga gctgcagaag aaggcggagc accaggtggg ggaagatggg      240

tttttactga agatcaagct ggggcactac gccacgcagc tccagaaaac atatgaccgc      300

tgccccctgg agctggtccg ctgcatccgg cacattctgt acaatgaaca gaggctggtc      360

cgagaagcca acaattgcag ctctccggct gggatcctgg ttgacgccat gtcccagaag      420

caccttcaga tcaaccagac atttgaggag ctgcgactgg tcacgcagga cacagagaat      480

gagctgaaga aactgcagca gactcaggag tacttcatca tccagtacca ggagagcctg      540

aggatccaag ctcagtttgc ccagctggcc cagctgagcc cccaggagcg tctgagccgg      600

gagacggccc tccagcagaa gcaggtgtct ctggaggcct ggttgcagcg tgaggcacag      660

acactgcagc agtaccgcgt ggagctggcc gagaagcacc agaagaccct gcagctgctg      720

cggaagcagc agaccatcat cctggatgac gagctgatcc agtggaagcg gcggcagcag      780

ctggccggga acggcgggcc ccccgagggc agcctggacg tgctacagtc ctggtgtgag      840

aagttggccg agatcatctg gcagaaccgg cagcagatcc gcagggctga gcgcctctgc      900

cagcagctgc ccatccccgg cccagtggag gagatgctgg ccgaggtcaa cgccaccatc      960

acggacatta tctcagccct ggtgaccagc acattcatca ttgagaagca gcctcctcag     1020

gtcctgaaga cccagaccaa gtttgcagcc accgtacgcc tgctggtggg cgggaagctg     1080

aacgtgcaca tgaatccccc ccaggtgaag gccaccatca tcagtgagca gcaggccaag     1140

tctctgctta aaaatgagaa cacccgcaac gagtgcagtg gtgagatcct gaacaactgc     1200

tgcgtgatgg agtaccacca agccacgggc accctcagtg cccacttcag gaacatgtca     1260

ctgaagagga tcaagcgtgc tgaccggcgg ggtgcagagt ccgtgacaga ggagaagttc     1320

acagtcctgt ttgagtctca gttcagtgtt ggcagcaatg agcttgtgtt ccaggtgaag     1380

actctgtccc tacctgtggt tgtcatcgtc cacggcagcc aggaccacaa tgccacggct     1440

actgtgctgt gggacaatgc ctttgctgag ccgggcaggg tgccatttgc cgtgcctgac     1500

aaagtgctgt ggccgcagct gtgtgaggcg ctcaacatga aattcaaggc cgaagtgcag     1560

agcaaccggg gcctgaccaa ggagaacctc gtgttcctgg cgcagaaact gttcaacaac     1620

agcagcagcc acctggagga ctacagtggc ctgtccgtgt cctggtccca gttcaacagg     1680

gagaacttgc cgggctggaa ctacaccttc tggcagtggt ttgacggggt gatggaggtg     1740

ttgaagaagc accacaagcc ccactggaat gatggggcca tcctaggttt tgtgaataag     1800

caacaggccc acgacctgct catcaacaag cccgacggga ccttcttgtt gcgctttagt     1860

gactcagaaa tcgggggcat caccatcgcc tggaagtttg attccccgga acgcaacctg     1920

tggaacctga aaccattcac cacgcgggat ttctccatca ggtccctggc tgaccggctg     1980

ggggacctga gctatctcat ctatgtgttt cctgaccgcc ccaaggatga ggtcttctcc     2040

aagtactaca ctcctgtgct ggctaaagct gttgatggat atgtgaaacc acagatcaag     2100

caagtggtcc ctgagtttgt gaatgcattt gcagatgctg ggggcagcag cgccacgtac     2160

atggaccagg ccccctcccc agctgtgtgc ccccaggctc cctataacat gtacccacag     2220

aaccctgacc atgtactcga tcaggatgga gaattcgacc tggatgagac catggatgtg     2280

gccaggcacg tggaggaact cttacgccga ccaatggaca gtcttgactc ccgcctctcg     2340

ccccctgccg gtcttttcac ctctgccaga ggctccctct ca                        2382


<210>  141
<211>  787
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  caSTAB5b: Mutation at N642H for constitutive activation

<400>  141

Met Ala Val Trp Ile Gln Ala Gln Gln Leu Gln Gly Glu Ala Leu His 
1               5                   10                  15      


Gln Met Gln Ala Leu Tyr Gly Gln His Phe Pro Ile Glu Val Arg His 
            20                  25                  30          


Tyr Leu Ser Gln Trp Ile Glu Ser Gln Ala Trp Asp Ser Val Asp Leu 
        35                  40                  45              


Asp Asn Pro Gln Glu Asn Ile Lys Ala Thr Gln Leu Leu Glu Gly Leu 
    50                  55                  60                  


Val Gln Glu Leu Gln Lys Lys Ala Glu His Gln Val Gly Glu Asp Gly 
65                  70                  75                  80  


Phe Leu Leu Lys Ile Lys Leu Gly His Tyr Ala Thr Gln Leu Gln Asn 
                85                  90                  95      


Thr Tyr Asp Arg Cys Pro Met Glu Leu Val Arg Cys Ile Arg His Ile 
            100                 105                 110         


Leu Tyr Asn Glu Gln Arg Leu Val Arg Glu Ala Asn Asn Gly Ser Ser 
        115                 120                 125             


Pro Ala Gly Ser Leu Ala Asp Ala Met Ser Gln Lys His Leu Gln Ile 
    130                 135                 140                 


Asn Gln Thr Phe Glu Glu Leu Arg Leu Val Thr Gln Asp Thr Glu Asn 
145                 150                 155                 160 


Glu Leu Lys Lys Leu Gln Gln Thr Gln Glu Tyr Phe Ile Ile Gln Tyr 
                165                 170                 175     


Gln Glu Ser Leu Arg Ile Gln Ala Gln Phe Gly Pro Leu Ala Gln Leu 
            180                 185                 190         


Ser Pro Gln Glu Arg Leu Ser Arg Glu Thr Ala Leu Gln Gln Lys Gln 
        195                 200                 205             


Val Ser Leu Glu Ala Trp Leu Gln Arg Glu Ala Gln Thr Leu Gln Gln 
    210                 215                 220                 


Tyr Arg Val Glu Leu Ala Glu Lys His Gln Lys Thr Leu Gln Leu Leu 
225                 230                 235                 240 


Arg Lys Gln Gln Thr Ile Ile Leu Asp Asp Glu Leu Ile Gln Trp Lys 
                245                 250                 255     


Arg Arg Gln Gln Leu Ala Gly Asn Gly Gly Pro Pro Glu Gly Ser Leu 
            260                 265                 270         


Asp Val Leu Gln Ser Trp Cys Glu Lys Leu Ala Glu Ile Ile Trp Gln 
        275                 280                 285             


Asn Arg Gln Gln Ile Arg Arg Ala Glu His Leu Cys Gln Gln Leu Pro 
    290                 295                 300                 


Ile Pro Gly Pro Val Glu Glu Met Leu Ala Glu Val Asn Ala Thr Ile 
305                 310                 315                 320 


Thr Asp Ile Ile Ser Ala Leu Val Thr Ser Thr Phe Ile Ile Glu Lys 
                325                 330                 335     


Gln Pro Pro Gln Val Leu Lys Thr Gln Thr Lys Phe Ala Ala Thr Val 
            340                 345                 350         


Arg Leu Leu Val Gly Gly Lys Leu Asn Val His Met Asn Pro Pro Gln 
        355                 360                 365             


Val Lys Ala Thr Ile Ile Ser Glu Gln Gln Ala Lys Ser Leu Leu Lys 
    370                 375                 380                 


Asn Glu Asn Thr Arg Asn Asp Tyr Ser Gly Glu Ile Leu Asn Asn Cys 
385                 390                 395                 400 


Cys Val Met Glu Tyr His Gln Ala Thr Gly Thr Leu Ser Ala His Phe 
                405                 410                 415     


Arg Asn Met Ser Leu Lys Arg Ile Lys Arg Ser Asp Arg Arg Gly Ala 
            420                 425                 430         


Glu Ser Val Thr Glu Glu Lys Phe Thr Ile Leu Phe Glu Ser Gln Phe 
        435                 440                 445             


Ser Val Gly Gly Asn Glu Leu Val Phe Gln Val Lys Thr Leu Ser Leu 
    450                 455                 460                 


Pro Val Val Val Ile Val His Gly Ser Gln Asp Asn Asn Ala Thr Ala 
465                 470                 475                 480 


Thr Val Leu Trp Asp Asn Ala Phe Ala Glu Pro Gly Arg Val Pro Phe 
                485                 490                 495     


Ala Val Pro Asp Lys Val Leu Trp Pro Gln Leu Cys Glu Ala Leu Asn 
            500                 505                 510         


Met Lys Phe Lys Ala Glu Val Gln Ser Asn Arg Gly Leu Thr Lys Glu 
        515                 520                 525             


Asn Leu Val Phe Leu Ala Gln Lys Leu Phe Asn Asn Ser Ser Ser His 
    530                 535                 540                 


Leu Glu Asp Tyr Ser Gly Leu Ser Val Ser Trp Ser Gln Phe Asn Arg 
545                 550                 555                 560 


Glu Asn Leu Pro Gly Arg Asn Tyr Thr Phe Trp Gln Trp Phe Asp Gly 
                565                 570                 575     


Val Met Glu Val Leu Lys Lys His Leu Lys Pro His Trp Asn Asp Gly 
            580                 585                 590         


Ala Ile Leu Gly Phe Val Asn Lys Gln Gln Ala His Asp Leu Leu Ile 
        595                 600                 605             


Asn Lys Pro Asp Gly Thr Phe Leu Leu Arg Phe Ser Asp Ser Glu Ile 
    610                 615                 620                 


Gly Gly Ile Thr Ile Ala Trp Lys Phe Asp Ser Gln Glu Arg Met Phe 
625                 630                 635                 640 


Trp His Leu Met Pro Phe Thr Thr Arg Asp Phe Ser Ile Arg Ser Leu 
                645                 650                 655     


Ala Asp Arg Leu Gly Asp Leu Asn Tyr Leu Ile Tyr Val Phe Pro Asp 
            660                 665                 670         


Arg Pro Lys Asp Glu Val Tyr Ser Lys Tyr Tyr Thr Pro Val Pro Cys 
        675                 680                 685             


Glu Ser Ala Thr Ala Lys Ala Val Asp Gly Tyr Val Lys Pro Gln Ile 
    690                 695                 700                 


Lys Gln Val Val Pro Glu Phe Val Asn Ala Ser Ala Asp Ala Gly Gly 
705                 710                 715                 720 


Gly Ser Ala Thr Tyr Met Asp Gln Ala Pro Ser Pro Ala Val Cys Pro 
                725                 730                 735     


Gln Ala His Tyr Asn Met Tyr Pro Gln Asn Pro Asp Ser Val Leu Asp 
            740                 745                 750         


Thr Asp Gly Asp Phe Asp Leu Glu Asp Thr Met Asp Val Ala Arg Arg 
        755                 760                 765             


Val Glu Glu Leu Leu Gly Arg Pro Met Asp Ser Gln Trp Ile Pro His 
    770                 775                 780                 


Ala Gln Ser 
785         


<210>  142
<211>  2361
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  caSTAB5b: Mutation at N642H for constitutive activation Coding 
       Sequence

<400>  142

Ala Thr Gly Gly Cys Thr Gly Thr Gly Thr Gly Gly Ala Thr Ala Cys 
1               5                   10                  15      


Ala Ala Gly Cys Thr Cys Ala Gly Cys Ala Gly Cys Thr Cys Cys Ala 
            20                  25                  30          


Ala Gly Gly Ala Gly Ala Ala Gly Cys Cys Cys Thr Thr Cys Ala Thr 
        35                  40                  45              


Cys Ala Gly Ala Thr Gly Cys Ala Ala Gly Cys Gly Thr Thr Ala Thr 
    50                  55                  60                  


Ala Thr Gly Gly Cys Cys Ala Gly Cys Ala Thr Thr Thr Thr Cys Cys 
65                  70                  75                  80  


Cys Ala Thr Thr Gly Ala Gly Gly Thr Gly Cys Gly Gly Cys Ala Thr 
                85                  90                  95      


Thr Ala Thr Thr Thr Ala Thr Cys Cys Cys Ala Gly Thr Gly Gly Ala 
            100                 105                 110         


Thr Thr Gly Ala Ala Ala Gly Cys Cys Ala Ala Gly Cys Ala Thr Gly 
        115                 120                 125             


Gly Gly Ala Cys Thr Cys Ala Gly Thr Ala Gly Ala Thr Cys Thr Thr 
    130                 135                 140                 


Gly Ala Thr Ala Ala Thr Cys Cys Ala Cys Ala Gly Gly Ala Gly Ala 
145                 150                 155                 160 


Ala Cys Ala Thr Thr Ala Ala Gly Gly Cys Cys Ala Cys Cys Cys Ala 
                165                 170                 175     


Gly Cys Thr Cys Cys Thr Gly Gly Ala Gly Gly Gly Cys Cys Thr Gly 
            180                 185                 190         


Gly Thr Gly Cys Ala Gly Gly Ala Gly Cys Thr Gly Cys Ala Gly Ala 
        195                 200                 205             


Ala Gly Ala Ala Gly Gly Cys Ala Gly Ala Gly Cys Ala Cys Cys Ala 
    210                 215                 220                 


Gly Gly Thr Gly Gly Gly Gly Gly Ala Ala Gly Ala Thr Gly Gly Gly 
225                 230                 235                 240 


Thr Thr Thr Thr Thr Ala Cys Thr Gly Ala Ala Gly Ala Thr Cys Ala 
                245                 250                 255     


Ala Gly Cys Thr Gly Gly Gly Gly Cys Ala Cys Thr Ala Thr Gly Cys 
            260                 265                 270         


Cys Ala Cys Ala Cys Ala Gly Cys Thr Cys Cys Ala Gly Ala Ala Cys 
        275                 280                 285             


Ala Cys Gly Thr Ala Thr Gly Ala Cys Cys Gly Cys Thr Gly Cys Cys 
    290                 295                 300                 


Cys Cys Ala Thr Gly Gly Ala Gly Cys Thr Gly Gly Thr Cys Cys Gly 
305                 310                 315                 320 


Cys Thr Gly Cys Ala Thr Cys Cys Gly Cys Cys Ala Thr Ala Thr Ala 
                325                 330                 335     


Thr Thr Gly Thr Ala Cys Ala Ala Thr Gly Ala Ala Cys Ala Gly Ala 
            340                 345                 350         


Gly Gly Thr Thr Gly Gly Thr Cys Cys Gly Ala Gly Ala Ala Gly Cys 
        355                 360                 365             


Cys Ala Ala Cys Ala Ala Thr Gly Gly Thr Ala Gly Cys Thr Cys Thr 
    370                 375                 380                 


Cys Cys Ala Gly Cys Thr Gly Gly Ala Ala Gly Cys Cys Thr Thr Gly 
385                 390                 395                 400 


Cys Thr Gly Ala Thr Gly Cys Cys Ala Thr Gly Thr Cys Cys Cys Ala 
                405                 410                 415     


Gly Ala Ala Ala Cys Ala Cys Cys Thr Cys Cys Ala Gly Ala Thr Cys 
            420                 425                 430         


Ala Ala Cys Cys Ala Gly Ala Cys Gly Thr Thr Thr Gly Ala Gly Gly 
        435                 440                 445             


Ala Gly Cys Thr Gly Cys Gly Ala Cys Thr Gly Gly Thr Cys Ala Cys 
    450                 455                 460                 


Gly Cys Ala Gly Gly Ala Cys Ala Cys Ala Gly Ala Gly Ala Ala Thr 
465                 470                 475                 480 


Gly Ala Gly Thr Thr Ala Ala Ala Ala Ala Ala Gly Cys Thr Gly Cys 
                485                 490                 495     


Ala Gly Cys Ala Gly Ala Cys Thr Cys Ala Gly Gly Ala Gly Thr Ala 
            500                 505                 510         


Cys Thr Thr Cys Ala Thr Cys Ala Thr Cys Cys Ala Gly Thr Ala Cys 
        515                 520                 525             


Cys Ala Gly Gly Ala Gly Ala Gly Cys Cys Thr Gly Ala Gly Gly Ala 
    530                 535                 540                 


Thr Cys Cys Ala Ala Gly Cys Thr Cys Ala Gly Thr Thr Thr Gly Gly 
545                 550                 555                 560 


Cys Cys Cys Gly Cys Thr Gly Gly Cys Cys Cys Ala Gly Cys Thr Gly 
                565                 570                 575     


Ala Gly Cys Cys Cys Cys Cys Ala Gly Gly Ala Gly Cys Gly Thr Cys 
            580                 585                 590         


Thr Gly Ala Gly Cys Cys Gly Gly Gly Ala Gly Ala Cys Gly Gly Cys 
        595                 600                 605             


Cys Cys Thr Cys Cys Ala Gly Cys Ala Gly Ala Ala Gly Cys Ala Ala 
    610                 615                 620                 


Gly Thr Gly Thr Cys Thr Cys Thr Gly Gly Ala Gly Gly Cys Cys Thr 
625                 630                 635                 640 


Gly Gly Thr Thr Gly Cys Ala Gly Cys Gly Thr Gly Ala Gly Gly Cys 
                645                 650                 655     


Ala Cys Ala Gly Ala Cys Ala Cys Thr Gly Cys Ala Gly Cys Ala Gly 
            660                 665                 670         


Thr Ala Cys Cys Gly Cys Gly Thr Gly Gly Ala Gly Cys Thr Gly Gly 
        675                 680                 685             


Cys Cys Gly Ala Gly Ala Ala Gly Cys Ala Cys Cys Ala Gly Ala Ala 
    690                 695                 700                 


Gly Ala Cys Cys Cys Thr Gly Cys Ala Gly Cys Thr Gly Cys Thr Gly 
705                 710                 715                 720 


Cys Gly Gly Ala Ala Gly Cys Ala Gly Cys Ala Gly Ala Cys Cys Ala 
                725                 730                 735     


Thr Cys Ala Thr Cys Cys Thr Gly Gly Ala Thr Gly Ala Cys Gly Ala 
            740                 745                 750         


Gly Cys Thr Gly Ala Thr Cys Cys Ala Gly Thr Gly Gly Ala Ala Gly 
        755                 760                 765             


Cys Gly Gly Cys Gly Gly Cys Ala Gly Cys Ala Gly Cys Thr Gly Gly 
    770                 775                 780                 


Cys Cys Gly Gly Gly Ala Ala Cys Gly Gly Cys Gly Gly Gly Cys Cys 
785                 790                 795                 800 


Cys Cys Cys Cys Gly Ala Gly Gly Gly Cys Ala Gly Cys Cys Thr Gly 
                805                 810                 815     


Gly Ala Cys Gly Thr Gly Cys Thr Ala Cys Ala Gly Thr Cys Cys Thr 
            820                 825                 830         


Gly Gly Thr Gly Thr Gly Ala Gly Ala Ala Gly Thr Thr Gly Gly Cys 
        835                 840                 845             


Cys Gly Ala Gly Ala Thr Cys Ala Thr Cys Thr Gly Gly Cys Ala Gly 
    850                 855                 860                 


Ala Ala Cys Cys Gly Gly Cys Ala Gly Cys Ala Gly Ala Thr Cys Cys 
865                 870                 875                 880 


Gly Cys Ala Gly Gly Gly Cys Thr Gly Ala Gly Cys Ala Cys Cys Thr 
                885                 890                 895     


Cys Thr Gly Cys Cys Ala Gly Cys Ala Gly Cys Thr Gly Cys Cys Cys 
            900                 905                 910         


Ala Thr Cys Cys Cys Cys Gly Gly Cys Cys Cys Ala Gly Thr Gly Gly 
        915                 920                 925             


Ala Gly Gly Ala Gly Ala Thr Gly Cys Thr Gly Gly Cys Cys Gly Ala 
    930                 935                 940                 


Gly Gly Thr Cys Ala Ala Cys Gly Cys Cys Ala Cys Cys Ala Thr Cys 
945                 950                 955                 960 


Ala Cys Gly Gly Ala Cys Ala Thr Thr Ala Thr Cys Thr Cys Ala Gly 
                965                 970                 975     


Cys Cys Cys Thr Gly Gly Thr Gly Ala Cys Cys Ala Gly Cys Ala Cys 
            980                 985                 990         


Gly Thr Thr Cys Ala Thr Cys Ala  Thr Thr Gly Ala Gly  Ala Ala Gly 
        995                 1000                 1005             


Cys Ala  Gly Cys Cys Thr Cys  Cys Thr Cys Ala Gly  Gly Thr Cys 
    1010                 1015                 1020             


Cys Thr  Gly Ala Ala Gly Ala  Cys Cys Cys Ala Gly  Ala Cys Cys 
    1025                 1030                 1035             


Ala Ala  Gly Thr Thr Thr Gly  Cys Ala Gly Cys Cys  Ala Cys Thr 
    1040                 1045                 1050             


Gly Thr  Gly Cys Gly Cys Cys  Thr Gly Cys Thr Gly  Gly Thr Gly 
    1055                 1060                 1065             


Gly Gly  Cys Gly Gly Gly Ala  Ala Gly Cys Thr Gly  Ala Ala Cys 
    1070                 1075                 1080             


Gly Thr  Gly Cys Ala Cys Ala  Thr Gly Ala Ala Cys  Cys Cys Cys 
    1085                 1090                 1095             


Cys Cys  Cys Cys Ala Gly Gly  Thr Gly Ala Ala Gly  Gly Cys Cys 
    1100                 1105                 1110             


Ala Cys  Cys Ala Thr Cys Ala  Thr Cys Ala Gly Thr  Gly Ala Gly 
    1115                 1120                 1125             


Cys Ala  Gly Cys Ala Gly Gly  Cys Cys Ala Ala Gly  Thr Cys Thr 
    1130                 1135                 1140             


Cys Thr  Gly Cys Thr Cys Ala  Ala Gly Ala Ala Cys  Gly Ala Gly 
    1145                 1150                 1155             


Ala Ala  Cys Ala Cys Cys Cys  Gly Cys Ala Ala Thr  Gly Ala Thr 
    1160                 1165                 1170             


Thr Ala  Cys Ala Gly Thr Gly  Gly Cys Gly Ala Gly  Ala Thr Cys 
    1175                 1180                 1185             


Thr Thr  Gly Ala Ala Cys Ala  Ala Cys Thr Gly Cys  Thr Gly Cys 
    1190                 1195                 1200             


Gly Thr  Cys Ala Thr Gly Gly  Ala Gly Thr Ala Cys  Cys Ala Cys 
    1205                 1210                 1215             


Cys Ala  Ala Gly Cys Cys Ala  Cys Ala Gly Gly Cys  Ala Cys Cys 
    1220                 1225                 1230             


Cys Thr  Thr Ala Gly Thr Gly  Cys Cys Cys Ala Cys  Thr Thr Cys 
    1235                 1240                 1245             


Ala Gly  Gly Ala Ala Thr Ala  Thr Gly Thr Cys Cys  Cys Thr Gly 
    1250                 1255                 1260             


Ala Ala  Ala Cys Gly Ala Ala  Thr Thr Ala Ala Gly  Ala Gly Gly 
    1265                 1270                 1275             


Thr Cys  Ala Gly Ala Cys Cys  Gly Thr Cys Gly Thr  Gly Gly Gly 
    1280                 1285                 1290             


Gly Cys  Ala Gly Ala Gly Thr  Cys Gly Gly Thr Gly  Ala Cys Ala 
    1295                 1300                 1305             


Gly Ala  Ala Gly Ala Ala Ala  Ala Ala Thr Thr Thr  Ala Cys Ala 
    1310                 1315                 1320             


Ala Thr  Cys Cys Thr Gly Thr  Thr Thr Gly Ala Ala  Thr Cys Cys 
    1325                 1330                 1335             


Cys Ala  Gly Thr Thr Cys Ala  Gly Thr Gly Thr Thr  Gly Gly Thr 
    1340                 1345                 1350             


Gly Gly  Ala Ala Ala Thr Gly  Ala Gly Cys Thr Gly  Gly Thr Thr 
    1355                 1360                 1365             


Thr Thr  Thr Cys Ala Ala Gly  Thr Cys Ala Ala Gly  Ala Cys Cys 
    1370                 1375                 1380             


Cys Thr  Gly Thr Cys Cys Cys  Thr Gly Cys Cys Ala  Gly Thr Gly 
    1385                 1390                 1395             


Gly Thr  Gly Gly Thr Gly Ala  Thr Cys Gly Thr Thr  Cys Ala Thr 
    1400                 1405                 1410             


Gly Gly  Cys Ala Gly Cys Cys  Ala Gly Gly Ala Cys  Ala Ala Cys 
    1415                 1420                 1425             


Ala Ala  Thr Gly Cys Gly Ala  Cys Gly Gly Cys Cys  Ala Cys Thr 
    1430                 1435                 1440             


Gly Thr  Thr Cys Thr Cys Thr  Gly Gly Gly Ala Cys  Ala Ala Thr 
    1445                 1450                 1455             


Gly Cys  Thr Thr Thr Thr Gly  Cys Ala Gly Ala Gly  Cys Cys Thr 
    1460                 1465                 1470             


Gly Gly  Cys Ala Gly Gly Gly  Thr Gly Cys Cys Ala  Thr Thr Thr 
    1475                 1480                 1485             


Gly Cys  Cys Gly Thr Gly Cys  Cys Thr Gly Ala Cys  Ala Ala Ala 
    1490                 1495                 1500             


Gly Thr  Gly Cys Thr Gly Thr  Gly Gly Cys Cys Ala  Cys Ala Gly 
    1505                 1510                 1515             


Cys Thr  Gly Thr Gly Thr Gly  Ala Gly Gly Cys Gly  Cys Thr Cys 
    1520                 1525                 1530             


Ala Ala  Cys Ala Thr Gly Ala  Ala Ala Thr Thr Cys  Ala Ala Gly 
    1535                 1540                 1545             


Gly Cys  Cys Gly Ala Ala Gly  Thr Gly Cys Ala Gly  Ala Gly Cys 
    1550                 1555                 1560             


Ala Ala  Cys Cys Gly Gly Gly  Gly Cys Cys Thr Gly  Ala Cys Cys 
    1565                 1570                 1575             


Ala Ala  Gly Gly Ala Gly Ala  Ala Cys Cys Thr Cys  Gly Thr Gly 
    1580                 1585                 1590             


Thr Thr  Cys Cys Thr Gly Gly  Cys Gly Cys Ala Gly  Ala Ala Ala 
    1595                 1600                 1605             


Cys Thr  Gly Thr Thr Cys Ala  Ala Cys Ala Ala Cys  Ala Gly Cys 
    1610                 1615                 1620             


Ala Gly  Cys Ala Gly Cys Cys  Ala Cys Cys Thr Gly  Gly Ala Gly 
    1625                 1630                 1635             


Gly Ala  Cys Thr Ala Cys Ala  Gly Thr Gly Gly Cys  Cys Thr Gly 
    1640                 1645                 1650             


Thr Cys  Thr Gly Thr Gly Thr  Cys Cys Thr Gly Gly  Thr Cys Cys 
    1655                 1660                 1665             


Cys Ala  Gly Thr Thr Cys Ala  Ala Cys Ala Gly Gly  Gly Ala Gly 
    1670                 1675                 1680             


Ala Ala  Thr Thr Thr Ala Cys  Cys Ala Gly Gly Ala  Cys Gly Gly 
    1685                 1690                 1695             


Ala Ala  Thr Thr Ala Cys Ala  Cys Thr Thr Thr Cys  Thr Gly Gly 
    1700                 1705                 1710             


Cys Ala  Ala Thr Gly Gly Thr  Thr Thr Gly Ala Cys  Gly Gly Thr 
    1715                 1720                 1725             


Gly Thr  Gly Ala Thr Gly Gly  Ala Ala Gly Thr Gly  Thr Thr Ala 
    1730                 1735                 1740             


Ala Ala  Ala Ala Ala Ala Cys  Ala Thr Cys Thr Cys  Ala Ala Gly 
    1745                 1750                 1755             


Cys Cys  Thr Cys Ala Thr Thr  Gly Gly Ala Ala Thr  Gly Ala Thr 
    1760                 1765                 1770             


Gly Gly  Gly Gly Cys Cys Ala  Thr Thr Thr Thr Gly  Gly Gly Gly 
    1775                 1780                 1785             


Thr Thr  Thr Gly Thr Ala Ala  Ala Cys Ala Ala Gly  Cys Ala Ala 
    1790                 1795                 1800             


Cys Ala  Gly Gly Cys Cys Cys  Ala Thr Gly Ala Cys  Cys Thr Ala 
    1805                 1810                 1815             


Cys Thr  Cys Ala Thr Thr Ala  Ala Cys Ala Ala Gly  Cys Cys Ala 
    1820                 1825                 1830             


Gly Ala  Thr Gly Gly Gly Ala  Cys Cys Thr Thr Cys  Cys Thr Cys 
    1835                 1840                 1845             


Cys Thr  Gly Ala Gly Ala Thr  Thr Cys Ala Gly Thr  Gly Ala Cys 
    1850                 1855                 1860             


Thr Cys  Ala Gly Ala Ala Ala  Thr Thr Gly Gly Cys  Gly Gly Cys 
    1865                 1870                 1875             


Ala Thr  Cys Ala Cys Cys Ala  Thr Thr Gly Cys Thr  Thr Gly Gly 
    1880                 1885                 1890             


Ala Ala  Gly Thr Thr Thr Gly  Ala Thr Thr Cys Thr  Cys Ala Gly 
    1895                 1900                 1905             


Gly Ala  Ala Ala Gly Ala Ala  Thr Gly Thr Thr Thr  Thr Gly Gly 
    1910                 1915                 1920             


Cys Ala  Cys Cys Thr Gly Ala  Thr Gly Cys Cys Thr  Thr Thr Thr 
    1925                 1930                 1935             


Ala Cys  Cys Ala Cys Cys Ala  Gly Ala Gly Ala Cys  Thr Thr Cys 
    1940                 1945                 1950             


Thr Cys  Cys Ala Thr Thr Cys  Gly Gly Thr Cys Cys  Cys Thr Ala 
    1955                 1960                 1965             


Gly Cys  Cys Gly Ala Cys Cys  Gly Cys Thr Thr Gly  Gly Gly Ala 
    1970                 1975                 1980             


Gly Ala  Cys Thr Thr Gly Ala  Ala Thr Thr Ala Cys  Cys Thr Thr 
    1985                 1990                 1995             


Ala Thr  Cys Thr Ala Cys Gly  Thr Gly Thr Thr Thr  Cys Cys Thr 
    2000                 2005                 2010             


Gly Ala  Thr Cys Gly Gly Cys  Cys Ala Ala Ala Ala  Gly Ala Thr 
    2015                 2020                 2025             


Gly Ala  Ala Gly Thr Ala Thr  Ala Cys Thr Cys Cys  Ala Ala Ala 
    2030                 2035                 2040             


Thr Ala  Cys Thr Ala Cys Ala  Cys Ala Cys Cys Ala  Gly Thr Thr 
    2045                 2050                 2055             


Cys Cys  Cys Thr Gly Cys Gly  Ala Gly Thr Cys Thr  Gly Cys Thr 
    2060                 2065                 2070             


Ala Cys  Thr Gly Cys Thr Ala  Ala Ala Gly Cys Thr  Gly Thr Thr 
    2075                 2080                 2085             


Gly Ala  Thr Gly Gly Ala Thr  Ala Cys Gly Thr Gly  Ala Ala Gly 
    2090                 2095                 2100             


Cys Cys  Ala Cys Ala Gly Ala  Thr Cys Ala Ala Gly  Cys Ala Ala 
    2105                 2110                 2115             


Gly Thr  Gly Gly Thr Cys Cys  Cys Thr Gly Ala Gly  Thr Thr Thr 
    2120                 2125                 2130             


Gly Thr  Gly Ala Ala Cys Gly  Cys Ala Thr Cys Thr  Gly Cys Ala 
    2135                 2140                 2145             


Gly Ala  Thr Gly Cys Cys Gly  Gly Gly Gly Gly Cys  Gly Gly Cys 
    2150                 2155                 2160             


Ala Gly  Cys Gly Cys Cys Ala  Cys Gly Thr Ala Cys  Ala Thr Gly 
    2165                 2170                 2175             


Gly Ala  Cys Cys Ala Gly Gly  Cys Cys Cys Cys Cys  Thr Cys Cys 
    2180                 2185                 2190             


Cys Cys  Ala Gly Cys Thr Gly  Thr Gly Thr Gly Thr  Cys Cys Cys 
    2195                 2200                 2205             


Cys Ala  Gly Gly Cys Thr Cys  Ala Cys Thr Ala Thr  Ala Ala Cys 
    2210                 2215                 2220             


Ala Thr  Gly Thr Ala Cys Cys  Cys Ala Cys Ala Gly  Ala Ala Cys 
    2225                 2230                 2235             


Cys Cys  Thr Gly Ala Cys Thr  Cys Ala Gly Thr Cys  Cys Thr Thr 
    2240                 2245                 2250             


Gly Ala  Cys Ala Cys Cys Gly  Ala Thr Gly Gly Gly  Gly Ala Cys 
    2255                 2260                 2265             


Thr Thr  Cys Gly Ala Thr Cys  Thr Gly Gly Ala Gly  Gly Ala Cys 
    2270                 2275                 2280             


Ala Cys  Ala Ala Thr Gly Gly  Ala Cys Gly Thr Ala  Gly Cys Gly 
    2285                 2290                 2295             


Cys Gly  Gly Cys Gly Thr Gly  Thr Gly Gly Ala Gly  Gly Ala Gly 
    2300                 2305                 2310             


Cys Thr  Cys Cys Thr Gly Gly  Gly Cys Cys Gly Gly  Cys Cys Ala 
    2315                 2320                 2325             


Ala Thr  Gly Gly Ala Cys Ala  Gly Thr Cys Ala Gly  Thr Gly Gly 
    2330                 2335                 2340             


Ala Thr  Cys Cys Cys Gly Cys  Ala Cys Gly Cys Ala  Cys Ala Ala 
    2345                 2350                 2355             


Thr Cys  Gly 
    2360     


<210>  143
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3-zeta ICD

<400>  143

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
    50                  55                  60                  


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
65                  70                  75                  80  


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
                85                  90                  95      


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
            100                 105                 110         


Arg 
    


<210>  144
<211>  331
<212>  PRT
<213>  Homo sapiens

<400>  144

Met Thr Ala Lys Met Glu Thr Thr Phe Tyr Asp Asp Ala Leu Asn Ala 
1               5                   10                  15      


Ser Phe Leu Pro Ser Glu Ser Gly Pro Tyr Gly Tyr Ser Asn Pro Lys 
            20                  25                  30          


Ile Leu Lys Gln Ser Met Thr Leu Asn Leu Ala Asp Pro Val Gly Ser 
        35                  40                  45              


Leu Lys Pro His Leu Arg Ala Lys Asn Ser Asp Leu Leu Thr Ser Pro 
    50                  55                  60                  


Asp Val Gly Leu Leu Lys Leu Ala Ser Pro Glu Leu Glu Arg Leu Ile 
65                  70                  75                  80  


Ile Gln Ser Ser Asn Gly His Ile Thr Thr Thr Pro Thr Pro Thr Gln 
                85                  90                  95      


Phe Leu Cys Pro Lys Asn Val Thr Asp Glu Gln Glu Gly Phe Ala Glu 
            100                 105                 110         


Gly Phe Val Arg Ala Leu Ala Glu Leu His Ser Gln Asn Thr Leu Pro 
        115                 120                 125             


Ser Val Thr Ser Ala Ala Gln Pro Val Asn Gly Ala Gly Met Val Ala 
    130                 135                 140                 


Pro Ala Val Ala Ser Val Ala Gly Gly Ser Gly Ser Gly Gly Phe Ser 
145                 150                 155                 160 


Ala Ser Leu His Ser Glu Pro Pro Val Tyr Ala Asn Leu Ser Asn Phe 
                165                 170                 175     


Asn Pro Gly Ala Leu Ser Ser Gly Gly Gly Ala Pro Ser Tyr Gly Ala 
            180                 185                 190         


Ala Gly Leu Ala Phe Pro Ala Gln Pro Gln Gln Gln Gln Gln Pro Pro 
        195                 200                 205             


His His Leu Pro Gln Gln Met Pro Val Gln His Pro Arg Leu Gln Ala 
    210                 215                 220                 


Leu Lys Glu Glu Pro Gln Thr Val Pro Glu Met Pro Gly Glu Thr Pro 
225                 230                 235                 240 


Pro Leu Ser Pro Ile Asp Met Glu Ser Gln Glu Arg Ile Lys Ala Glu 
                245                 250                 255     


Arg Lys Arg Met Arg Asn Arg Ile Ala Ala Ser Lys Cys Arg Lys Arg 
            260                 265                 270         


Lys Leu Glu Arg Ile Ala Arg Leu Glu Glu Lys Val Lys Thr Leu Lys 
        275                 280                 285             


Ala Gln Asn Ser Glu Leu Ala Ser Thr Ala Asn Met Leu Arg Glu Gln 
    290                 295                 300                 


Val Ala Gln Leu Lys Gln Lys Val Met Asn His Val Asn Ser Gly Cys 
305                 310                 315                 320 


Gln Leu Met Leu Thr Gln Gln Leu Gln Thr Phe 
                325                 330     


<210>  145
<211>  439
<212>  PRT
<213>  Homo sapiens

<400>  145

Met Pro Leu Asn Val Ser Phe Thr Asn Arg Asn Tyr Asp Leu Asp Tyr 
1               5                   10                  15      


Asp Ser Val Gln Pro Tyr Phe Tyr Cys Asp Glu Glu Glu Asn Phe Tyr 
            20                  25                  30          


Gln Gln Gln Gln Gln Ser Glu Leu Gln Pro Pro Ala Pro Ser Glu Asp 
        35                  40                  45              


Ile Trp Lys Lys Phe Glu Leu Leu Pro Thr Pro Pro Leu Ser Pro Ser 
    50                  55                  60                  


Arg Arg Ser Gly Leu Cys Ser Pro Ser Tyr Val Ala Val Thr Pro Phe 
65                  70                  75                  80  


Ser Leu Arg Gly Asp Asn Asp Gly Gly Gly Gly Ser Phe Ser Thr Ala 
                85                  90                  95      


Asp Gln Leu Glu Met Val Thr Glu Leu Leu Gly Gly Asp Met Val Asn 
            100                 105                 110         


Gln Ser Phe Ile Cys Asp Pro Asp Asp Glu Thr Phe Ile Lys Asn Ile 
        115                 120                 125             


Ile Ile Gln Asp Cys Met Trp Ser Gly Phe Ser Ala Ala Ala Lys Leu 
    130                 135                 140                 


Val Ser Glu Lys Leu Ala Ser Tyr Gln Ala Ala Arg Lys Asp Ser Gly 
145                 150                 155                 160 


Ser Pro Asn Pro Ala Arg Gly His Ser Val Cys Ser Thr Ser Ser Leu 
                165                 170                 175     


Tyr Leu Gln Asp Leu Ser Ala Ala Ala Ser Glu Cys Ile Asp Pro Ser 
            180                 185                 190         


Val Val Phe Pro Tyr Pro Leu Asn Asp Ser Ser Ser Pro Lys Ser Cys 
        195                 200                 205             


Ala Ser Gln Asp Ser Ser Ala Phe Ser Pro Ser Ser Asp Ser Leu Leu 
    210                 215                 220                 


Ser Ser Thr Glu Ser Ser Pro Gln Gly Ser Pro Glu Pro Leu Val Leu 
225                 230                 235                 240 


His Glu Glu Thr Pro Pro Thr Thr Ser Ser Asp Ser Glu Glu Glu Gln 
                245                 250                 255     


Glu Asp Glu Glu Glu Ile Asp Val Val Ser Val Glu Lys Arg Gln Ala 
            260                 265                 270         


Pro Gly Lys Arg Ser Glu Ser Gly Ser Pro Ser Ala Gly Gly His Ser 
        275                 280                 285             


Lys Pro Pro His Ser Pro Leu Val Leu Lys Arg Cys His Val Ser Thr 
    290                 295                 300                 


His Gln His Asn Tyr Ala Ala Pro Pro Ser Thr Arg Lys Asp Tyr Pro 
305                 310                 315                 320 


Ala Ala Lys Arg Val Lys Leu Asp Ser Val Arg Val Leu Arg Gln Ile 
                325                 330                 335     


Ser Asn Asn Arg Lys Cys Thr Ser Pro Arg Ser Ser Asp Thr Glu Glu 
            340                 345                 350         


Asn Val Lys Arg Arg Thr His Asn Val Leu Glu Arg Gln Arg Arg Asn 
        355                 360                 365             


Glu Leu Lys Arg Ser Phe Phe Ala Leu Arg Asp Gln Ile Pro Glu Leu 
    370                 375                 380                 


Glu Asn Asn Glu Lys Ala Pro Lys Val Val Ile Leu Lys Lys Ala Thr 
385                 390                 395                 400 


Ala Tyr Ile Leu Ser Val Gln Ala Glu Glu Gln Lys Leu Ile Ser Glu 
                405                 410                 415     


Glu Asp Leu Leu Arg Lys Arg Arg Glu Gln Leu Lys His Lys Leu Glu 
            420                 425                 430         


Gln Leu Arg Asn Ser Cys Ala 
        435                 


<210>  146
<211>  384
<212>  PRT
<213>  Homo sapiens

<400>  146

Met Pro Gln Leu Asp Ser Gly Gly Gly Gly Ala Gly Gly Gly Asp Asp 
1               5                   10                  15      


Leu Gly Ala Pro Asp Glu Leu Leu Ala Phe Gln Asp Glu Gly Glu Glu 
            20                  25                  30          


Gln Asp Asp Lys Ser Arg Asp Ser Ala Ala Gly Pro Glu Arg Asp Leu 
        35                  40                  45              


Ala Glu Leu Lys Ser Ser Leu Val Asn Glu Ser Glu Gly Ala Ala Gly 
    50                  55                  60                  


Gly Ala Gly Ile Pro Gly Val Pro Gly Ala Gly Ala Gly Ala Arg Gly 
65                  70                  75                  80  


Glu Ala Glu Ala Leu Gly Arg Glu His Ala Ala Gln Arg Leu Phe Pro 
                85                  90                  95      


Asp Lys Leu Pro Glu Pro Leu Glu Asp Gly Leu Lys Ala Pro Glu Cys 
            100                 105                 110         


Thr Ser Gly Met Tyr Lys Glu Thr Val Tyr Ser Ala Phe Asn Leu Leu 
        115                 120                 125             


Met His Tyr Pro Pro Pro Ser Gly Ala Gly Gln His Pro Gln Pro Gln 
    130                 135                 140                 


Pro Pro Leu His Lys Ala Asn Gln Pro Pro His Gly Val Pro Gln Leu 
145                 150                 155                 160 


Ser Leu Tyr Glu His Phe Asn Ser Pro His Pro Thr Pro Ala Pro Ala 
                165                 170                 175     


Asp Ile Ser Gln Lys Gln Val His Arg Pro Leu Gln Thr Pro Asp Leu 
            180                 185                 190         


Ser Gly Phe Tyr Ser Leu Thr Ser Gly Ser Met Gly Gln Leu Pro His 
        195                 200                 205             


Thr Val Ser Trp Phe Thr His Pro Ser Leu Met Leu Gly Ser Gly Val 
    210                 215                 220                 


Pro Gly His Pro Ala Ala Ile Pro His Pro Ala Ile Val Pro Pro Ser 
225                 230                 235                 240 


Gly Lys Gln Glu Leu Gln Pro Phe Asp Arg Asn Leu Lys Thr Gln Ala 
                245                 250                 255     


Glu Ser Lys Ala Glu Lys Glu Ala Lys Lys Pro Thr Ile Lys Lys Pro 
            260                 265                 270         


Leu Asn Ala Phe Met Leu Tyr Met Lys Glu Met Arg Ala Lys Val Ile 
        275                 280                 285             


Ala Glu Cys Thr Leu Lys Glu Ser Ala Ala Ile Asn Gln Ile Leu Gly 
    290                 295                 300                 


Arg Arg Trp His Ala Leu Ser Arg Glu Glu Gln Ala Lys Tyr Tyr Glu 
305                 310                 315                 320 


Leu Ala Arg Lys Glu Arg Gln Leu His Met Gln Leu Tyr Pro Gly Trp 
                325                 330                 335     


Ser Ala Arg Asp Asn Tyr Gly Lys Lys Lys Arg Arg Ser Arg Glu Lys 
            340                 345                 350         


His Gln Glu Ser Thr Thr Glu Thr Asn Trp Pro Arg Glu Leu Lys Asp 
        355                 360                 365             


Gly Asn Gly Gln Glu Ser Leu Ser Met Ser Ser Ser Ser Ser Pro Ala 
    370                 375                 380                 


<210>  147
<211>  706
<212>  PRT
<213>  Homo sapiens

<400>  147

Met Ala Ser Pro Ala Asp Ser Cys Ile Gln Phe Thr Arg His Ala Ser 
1               5                   10                  15      


Asp Val Leu Leu Asn Leu Asn Arg Leu Arg Ser Arg Asp Ile Leu Thr 
            20                  25                  30          


Asp Val Val Ile Val Val Ser Arg Glu Gln Phe Arg Ala His Lys Thr 
        35                  40                  45              


Val Leu Met Ala Cys Ser Gly Leu Phe Tyr Ser Ile Phe Thr Asp Gln 
    50                  55                  60                  


Leu Lys Cys Asn Leu Ser Val Ile Asn Leu Asp Pro Glu Ile Asn Pro 
65                  70                  75                  80  


Glu Gly Phe Cys Ile Leu Leu Asp Phe Met Tyr Thr Ser Arg Leu Asn 
                85                  90                  95      


Leu Arg Glu Gly Asn Ile Met Ala Val Met Ala Thr Ala Met Tyr Leu 
            100                 105                 110         


Gln Met Glu His Val Val Asp Thr Cys Arg Lys Phe Ile Lys Ala Ser 
        115                 120                 125             


Glu Ala Glu Met Val Ser Ala Ile Lys Pro Pro Arg Glu Glu Phe Leu 
    130                 135                 140                 


Asn Ser Arg Met Leu Met Pro Gln Asp Ile Met Ala Tyr Arg Gly Arg 
145                 150                 155                 160 


Glu Val Val Glu Asn Asn Leu Pro Leu Arg Ser Ala Pro Gly Cys Glu 
                165                 170                 175     


Ser Arg Ala Phe Ala Pro Ser Leu Tyr Ser Gly Leu Ser Thr Pro Pro 
            180                 185                 190         


Ala Ser Tyr Ser Met Tyr Ser His Leu Pro Val Ser Ser Leu Leu Phe 
        195                 200                 205             


Ser Asp Glu Glu Phe Arg Asp Val Arg Met Pro Val Ala Asn Pro Phe 
    210                 215                 220                 


Pro Lys Glu Arg Ala Leu Pro Cys Asp Ser Ala Arg Pro Val Pro Gly 
225                 230                 235                 240 


Glu Tyr Ser Arg Pro Thr Leu Glu Val Ser Pro Asn Val Cys His Ser 
                245                 250                 255     


Asn Ile Tyr Ser Pro Lys Glu Thr Ile Pro Glu Glu Ala Arg Ser Asp 
            260                 265                 270         


Met His Tyr Ser Val Ala Glu Gly Leu Lys Pro Ala Ala Pro Ser Ala 
        275                 280                 285             


Arg Asn Ala Pro Tyr Phe Pro Cys Asp Lys Ala Ser Lys Glu Glu Glu 
    290                 295                 300                 


Arg Pro Ser Ser Glu Asp Glu Ile Ala Leu His Phe Glu Pro Pro Asn 
305                 310                 315                 320 


Ala Pro Leu Asn Arg Lys Gly Leu Val Ser Pro Gln Ser Pro Gln Lys 
                325                 330                 335     


Ser Asp Cys Gln Pro Asn Ser Pro Thr Glu Ser Cys Ser Ser Lys Asn 
            340                 345                 350         


Ala Cys Ile Leu Gln Ala Ser Gly Ser Pro Pro Ala Lys Ser Pro Thr 
        355                 360                 365             


Asp Pro Lys Ala Cys Asn Trp Lys Lys Tyr Lys Phe Ile Val Leu Asn 
    370                 375                 380                 


Ser Leu Asn Gln Asn Ala Lys Pro Glu Gly Pro Glu Gln Ala Glu Leu 
385                 390                 395                 400 


Gly Arg Leu Ser Pro Arg Ala Tyr Thr Ala Pro Pro Ala Cys Gln Pro 
                405                 410                 415     


Pro Met Glu Pro Glu Asn Leu Asp Leu Gln Ser Pro Thr Lys Leu Ser 
            420                 425                 430         


Ala Ser Gly Glu Asp Ser Thr Ile Pro Gln Ala Ser Arg Leu Asn Asn 
        435                 440                 445             


Ile Val Asn Arg Ser Met Thr Gly Ser Pro Arg Ser Ser Ser Glu Ser 
    450                 455                 460                 


His Ser Pro Leu Tyr Met His Pro Pro Lys Cys Thr Ser Cys Gly Ser 
465                 470                 475                 480 


Gln Ser Pro Gln His Ala Glu Met Cys Leu His Thr Ala Gly Pro Thr 
                485                 490                 495     


Phe Pro Glu Glu Met Gly Glu Thr Gln Ser Glu Tyr Ser Asp Ser Ser 
            500                 505                 510         


Cys Glu Asn Gly Ala Phe Phe Cys Asn Glu Cys Asp Cys Arg Phe Ser 
        515                 520                 525             


Glu Glu Ala Ser Leu Lys Arg His Thr Leu Gln Thr His Ser Asp Lys 
    530                 535                 540                 


Pro Tyr Lys Cys Asp Arg Cys Gln Ala Ser Phe Arg Tyr Lys Gly Asn 
545                 550                 555                 560 


Leu Ala Ser His Lys Thr Val His Thr Gly Glu Lys Pro Tyr Arg Cys 
                565                 570                 575     


Asn Ile Cys Gly Ala Gln Phe Asn Arg Pro Ala Asn Leu Lys Thr His 
            580                 585                 590         


Thr Arg Ile His Ser Gly Glu Lys Pro Tyr Lys Cys Glu Thr Cys Gly 
        595                 600                 605             


Ala Arg Phe Val Gln Val Ala His Leu Arg Ala His Val Leu Ile His 
    610                 615                 620                 


Thr Gly Glu Lys Pro Tyr Pro Cys Glu Ile Cys Gly Thr Arg Phe Arg 
625                 630                 635                 640 


His Leu Gln Thr Leu Lys Ser His Leu Arg Ile His Thr Gly Glu Lys 
                645                 650                 655     


Pro Tyr His Cys Glu Lys Cys Asn Leu His Phe Arg His Lys Ser Gln 
            660                 665                 670         


Leu Arg Leu His Leu Arg Gln Lys His Gly Ala Ile Thr Asn Thr Lys 
        675                 680                 685             


Val Gln Tyr Arg Val Ser Ala Thr Asp Leu Pro Pro Glu Leu Pro Lys 
    690                 695                 700                 


Ala Cys 
705     


<210>  148
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (Gly4Ser)n linker sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(5)
<223>  Can be repeated n times, wherin n is an integer of 1, 2, 3, 4, 5,
       6, 7, 8, 9 or 10

<400>  148

Gly Gly Gly Gly Ser 
1               5   


<210>  149
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OX40 ICD

<400>  149

Ala Leu Tyr Leu Leu Arg Arg Asp Gln Arg Leu Pro Pro Asp Ala His 
1               5                   10                  15      


Lys Pro Pro Gly Gly Gly Ser Phe Arg Thr Pro Ile Gln Glu Glu Gln 
            20                  25                  30          


Ala Asp Ala His Ser Thr Leu Ala Lys Ile 
        35                  40          


<210>  150
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (Gly3Ser)n linker sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(4)
<223>  Can be repeated n times, wherin n is an integer of 1, 2, 3, 4, 5,
       6, 7, 8, 9 or 10

<400>  150

Gly Gly Gly Ser 
1               


<210>  151
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (GGGG)n linker sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(4)
<223>  Can be repeated n times, wherin n is an integer of 1, 2, 3, 4, 5,
       6, 7, 8, 9 or 10

<400>  151

Gly Gly Gly Gly 
1               


<210>  152
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (GSAGSAAGSGEF)n linker sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(12)
<223>  Can be repeated n times, wherin n is an integer of 1, 2, 3, 4, 5,
       6, 7, 8, 9 or 10

<400>  152

Gly Ser Ala Gly Ser Ala Ala Gly Ser Gly Glu Phe 
1               5                   10          


<210>  153
<211>  48
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD27 ICD

<400>  153

Gln Arg Arg Lys Tyr Arg Ser Asn Lys Gly Glu Ser Pro Val Glu Pro 
1               5                   10                  15      


Ala Glu Pro Cys His Tyr Ser Cys Pro Arg Glu Glu Glu Gly Ser Thr 
            20                  25                  30          


Ile Pro Ile Gln Glu Asp Tyr Arg Lys Pro Glu Pro Ala Cys Ser Pro 
        35                  40                  45              


<210>  154
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (Gly3Ser)n(Gly4Ser)n linker sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(4)
<223>  Can be repeated n times, wherin n is an integer of 1, 2, 3, 4, 5,
       6, 7, 8, 9 or 10

<220>
<221>  MISC_FEATURE
<222>  (5)..(9)
<223>  Can be repeated n times, wherin n is an integer of 1, 2, 3, 4, 5,
       6, 7, 8, 9 or 10

<400>  154

Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   


<210>  155
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (Gly3Ser)n(Gly2Ser)n linker sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(4)
<223>  Can be repeated n times, wherin n is an integer of 1, 2, 3, 4, 5,
       6, 7, 8, 9 or 10

<220>
<221>  MISC_FEATURE
<222>  (5)..(7)
<223>  Can be repeated n times, wherin n is an integer of 1, 2, 3, 4, 5,
       6, 7, 8, 9 or 10

<400>  155

Gly Gly Gly Ser Gly Gly Ser 
1               5           


<210>  156
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (Gly3Ser)n(Gly4Ser)1 linker sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(4)
<223>  Can be repeated n times, wherin n is an integer of 1, 2, 3, 4, 5,
       6, 7, 8, 9 or 10

<400>  156

Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   


<210>  157

<400>  157
000

<210>  158
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (Gly4Ser)4 linker sequence

<400>  158

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser 
            20  


<210>  159
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (Gly4Ser)3 linker sequence

<400>  159

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  160
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (Gly4Ser)2 linker sequence

<400>  160

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10  


<210>  161

<400>  161
000

<210>  162
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (Gly4Ser)1 linker sequence

<400>  162

Gly Gly Gly Gly Ser 
1               5   


<210>  163
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (Gly3Ser)2 linker sequence

<400>  163

Gly Gly Gly Ser Gly Gly Gly Ser 
1               5               


<210>  164
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (Gly3Ser)1 linker sequence

<400>  164

Gly Gly Gly Ser 
1               


<210>  165

<400>  165
000

<210>  166
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (Gly2Ser)2 linker sequence

<400>  166

Gly Gly Ser Gly Gly Ser 
1               5       


<210>  167
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GGSGGGSGGSG linker sequence

<400>  167

Gly Gly Ser Gly Gly Gly Ser Gly Gly Ser Gly 
1               5                   10      


<210>  168
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GGSGGGSGSG linker sequence

<400>  168

Gly Gly Ser Gly Gly Gly Ser Gly Ser Gly 
1               5                   10  


<210>  169

<400>  169
000

<210>  170
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GGSGGGSG linker sequence

<400>  170

Gly Gly Ser Gly Gly Gly Ser Gly 
1               5               


<210>  171

<400>  171
000

<210>  172

<400>  172
000

<210>  173

<400>  173
000

<210>  174

<400>  174
000

<210>  175

<400>  175
000

<210>  176

<400>  176
000

<210>  177

<400>  177
000

<210>  178

<400>  178
000

<210>  179

<400>  179
000

<210>  180
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 ICD (residues 180-220 of UniProt ID P10747)

<400>  180

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  181
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 ICD

<400>  181

Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  182
<211>  123
<212>  DNA
<213>  Homo sapiens

<400>  182
aggagtaaga ggagcaggct cctgcacagt gactacatga acatgactcc ccgccgcccc       60

gggcccaccc gcaagcatta ccagccctat gccccaccac gcgacttcgc agcctatcgc      120

tcc                                                                    123


<210>  183
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Degron sequence

<400>  183

Arg Arg Arg Gly 
1               


<210>  184
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Degron sequence #2

<400>  184

Arg Arg Arg Gly Asn 
1               5   


