               SEQUENCE LISTING

<110> UNIVERSITY OF ROCHESTER
      THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
      ALBERT EINSTEIN COLLEGE OF MEDICINE
      GORBUNOVA Vera
      SELUANOV Andrei
      SUH Yousin

<120> VARIANTS OF SIRT6 FOR USE IN PREVENTING AND/OR TREATING AGE-RELATED DISEASES

<130> IBIO-1826/PCT

<150> US63/188,573
<151> 2021-05-14

<160> 26

<170> BiSSAP 1.3.6

<210> 1
<211> 355
<212> PRT
<213> Homo sapiens


<220> 
<223> Wild type SIRT6 amino acid sequence

<400> 1
Met Ser Val Asn Tyr Ala Ala Gly Leu Ser Pro Tyr Ala Asp Lys Gly 
1               5                   10                  15      
Lys Cys Gly Leu Pro Glu Ile Phe Asp Pro Pro Glu Glu Leu Glu Arg 
            20                  25                  30          
Lys Val Trp Glu Leu Ala Arg Leu Val Trp Gln Ser Ser Ser Val Val 
        35                  40                  45              
Phe His Thr Gly Ala Gly Ile Ser Thr Ala Ser Gly Ile Pro Asp Phe 
    50                  55                  60                  
Arg Gly Pro His Gly Val Trp Thr Met Glu Glu Arg Gly Leu Ala Pro 
65                  70                  75                  80  
Lys Phe Asp Thr Thr Phe Glu Ser Ala Arg Pro Thr Gln Thr His Met 
                85                  90                  95      
Ala Leu Val Gln Leu Glu Arg Val Gly Leu Leu Arg Phe Leu Val Ser 
            100                 105                 110         
Gln Asn Val Asp Gly Leu His Val Arg Ser Gly Phe Pro Arg Asp Lys 
        115                 120                 125             
Leu Ala Glu Leu His Gly Asn Met Phe Val Glu Glu Cys Ala Lys Cys 
    130                 135                 140                 
Lys Thr Gln Tyr Val Arg Asp Thr Val Val Gly Thr Met Gly Leu Lys 
145                 150                 155                 160 
Ala Thr Gly Arg Leu Cys Thr Val Ala Lys Ala Arg Gly Leu Arg Ala 
                165                 170                 175     
Cys Arg Gly Glu Leu Arg Asp Thr Ile Leu Asp Trp Glu Asp Ser Leu 
            180                 185                 190         
Pro Asp Arg Asp Leu Ala Leu Ala Asp Glu Ala Ser Arg Asn Ala Asp 
        195                 200                 205             
Leu Ser Ile Thr Leu Gly Thr Ser Leu Gln Ile Arg Pro Ser Gly Asn 
    210                 215                 220                 
Leu Pro Leu Ala Thr Lys Arg Arg Gly Gly Arg Leu Val Ile Val Asn 
225                 230                 235                 240 
Leu Gln Pro Thr Lys His Asp Arg His Ala Asp Leu Arg Ile His Gly 
                245                 250                 255     
Tyr Val Asp Glu Val Met Thr Arg Leu Met Lys His Leu Gly Leu Glu 
            260                 265                 270         
Ile Pro Ala Trp Asp Gly Pro Arg Val Leu Glu Arg Ala Leu Pro Pro 
        275                 280                 285             
Leu Pro Arg Pro Pro Thr Pro Lys Leu Glu Pro Lys Glu Glu Ser Pro 
    290                 295                 300                 
Thr Arg Ile Asn Gly Ser Ile Pro Ala Gly Pro Lys Gln Glu Pro Cys 
305                 310                 315                 320 
Ala Gln His Asn Gly Ser Glu Pro Ala Ser Pro Lys Arg Glu Arg Pro 
                325                 330                 335     
Thr Ser Pro Ala Pro His Arg Pro Pro Lys Arg Val Lys Ala Lys Ala 
            340                 345                 350         
Val Pro Ser 
        355 

<210> 2
<211> 355
<212> PRT
<213> Artificial Sequence


<220> 
<223> SIRT6 N308K variant amino acid sequence

<400> 2
Met Ser Val Asn Tyr Ala Ala Gly Leu Ser Pro Tyr Ala Asp Lys Gly 
1               5                   10                  15      
Lys Cys Gly Leu Pro Glu Ile Phe Asp Pro Pro Glu Glu Leu Glu Arg 
            20                  25                  30          
Lys Val Trp Glu Leu Ala Arg Leu Val Trp Gln Ser Ser Ser Val Val 
        35                  40                  45              
Phe His Thr Gly Ala Gly Ile Ser Thr Ala Ser Gly Ile Pro Asp Phe 
    50                  55                  60                  
Arg Gly Pro His Gly Val Trp Thr Met Glu Glu Arg Gly Leu Ala Pro 
65                  70                  75                  80  
Lys Phe Asp Thr Thr Phe Glu Ser Ala Arg Pro Thr Gln Thr His Met 
                85                  90                  95      
Ala Leu Val Gln Leu Glu Arg Val Gly Leu Leu Arg Phe Leu Val Ser 
            100                 105                 110         
Gln Asn Val Asp Gly Leu His Val Arg Ser Gly Phe Pro Arg Asp Lys 
        115                 120                 125             
Leu Ala Glu Leu His Gly Asn Met Phe Val Glu Glu Cys Ala Lys Cys 
    130                 135                 140                 
Lys Thr Gln Tyr Val Arg Asp Thr Val Val Gly Thr Met Gly Leu Lys 
145                 150                 155                 160 
Ala Thr Gly Arg Leu Cys Thr Val Ala Lys Ala Arg Gly Leu Arg Ala 
                165                 170                 175     
Cys Arg Gly Glu Leu Arg Asp Thr Ile Leu Asp Trp Glu Asp Ser Leu 
            180                 185                 190         
Pro Asp Arg Asp Leu Ala Leu Ala Asp Glu Ala Ser Arg Asn Ala Asp 
        195                 200                 205             
Leu Ser Ile Thr Leu Gly Thr Ser Leu Gln Ile Arg Pro Ser Gly Asn 
    210                 215                 220                 
Leu Pro Leu Ala Thr Lys Arg Arg Gly Gly Arg Leu Val Ile Val Asn 
225                 230                 235                 240 
Leu Gln Pro Thr Lys His Asp Arg His Ala Asp Leu Arg Ile His Gly 
                245                 250                 255     
Tyr Val Asp Glu Val Met Thr Arg Leu Met Lys His Leu Gly Leu Glu 
            260                 265                 270         
Ile Pro Ala Trp Asp Gly Pro Arg Val Leu Glu Arg Ala Leu Pro Pro 
        275                 280                 285             
Leu Pro Arg Pro Pro Thr Pro Lys Leu Glu Pro Lys Glu Glu Ser Pro 
    290                 295                 300                 
Thr Arg Ile Lys Gly Ser Ile Pro Ala Gly Pro Lys Gln Glu Pro Cys 
305                 310                 315                 320 
Ala Gln His Asn Gly Ser Glu Pro Ala Ser Pro Lys Arg Glu Arg Pro 
                325                 330                 335     
Thr Ser Pro Ala Pro His Arg Pro Pro Lys Arg Val Lys Ala Lys Ala 
            340                 345                 350         
Val Pro Ser 
        355 

<210> 3
<211> 355
<212> PRT
<213> Artificial Sequence


<220> 
<223> SIRT6 A313S variant amino acid sequence

<400> 3
Met Ser Val Asn Tyr Ala Ala Gly Leu Ser Pro Tyr Ala Asp Lys Gly 
1               5                   10                  15      
Lys Cys Gly Leu Pro Glu Ile Phe Asp Pro Pro Glu Glu Leu Glu Arg 
            20                  25                  30          
Lys Val Trp Glu Leu Ala Arg Leu Val Trp Gln Ser Ser Ser Val Val 
        35                  40                  45              
Phe His Thr Gly Ala Gly Ile Ser Thr Ala Ser Gly Ile Pro Asp Phe 
    50                  55                  60                  
Arg Gly Pro His Gly Val Trp Thr Met Glu Glu Arg Gly Leu Ala Pro 
65                  70                  75                  80  
Lys Phe Asp Thr Thr Phe Glu Ser Ala Arg Pro Thr Gln Thr His Met 
                85                  90                  95      
Ala Leu Val Gln Leu Glu Arg Val Gly Leu Leu Arg Phe Leu Val Ser 
            100                 105                 110         
Gln Asn Val Asp Gly Leu His Val Arg Ser Gly Phe Pro Arg Asp Lys 
        115                 120                 125             
Leu Ala Glu Leu His Gly Asn Met Phe Val Glu Glu Cys Ala Lys Cys 
    130                 135                 140                 
Lys Thr Gln Tyr Val Arg Asp Thr Val Val Gly Thr Met Gly Leu Lys 
145                 150                 155                 160 
Ala Thr Gly Arg Leu Cys Thr Val Ala Lys Ala Arg Gly Leu Arg Ala 
                165                 170                 175     
Cys Arg Gly Glu Leu Arg Asp Thr Ile Leu Asp Trp Glu Asp Ser Leu 
            180                 185                 190         
Pro Asp Arg Asp Leu Ala Leu Ala Asp Glu Ala Ser Arg Asn Ala Asp 
        195                 200                 205             
Leu Ser Ile Thr Leu Gly Thr Ser Leu Gln Ile Arg Pro Ser Gly Asn 
    210                 215                 220                 
Leu Pro Leu Ala Thr Lys Arg Arg Gly Gly Arg Leu Val Ile Val Asn 
225                 230                 235                 240 
Leu Gln Pro Thr Lys His Asp Arg His Ala Asp Leu Arg Ile His Gly 
                245                 250                 255     
Tyr Val Asp Glu Val Met Thr Arg Leu Met Lys His Leu Gly Leu Glu 
            260                 265                 270         
Ile Pro Ala Trp Asp Gly Pro Arg Val Leu Glu Arg Ala Leu Pro Pro 
        275                 280                 285             
Leu Pro Arg Pro Pro Thr Pro Lys Leu Glu Pro Lys Glu Glu Ser Pro 
    290                 295                 300                 
Thr Arg Ile Asn Gly Ser Ile Pro Ser Gly Pro Lys Gln Glu Pro Cys 
305                 310                 315                 320 
Ala Gln His Asn Gly Ser Glu Pro Ala Ser Pro Lys Arg Glu Arg Pro 
                325                 330                 335     
Thr Ser Pro Ala Pro His Arg Pro Pro Lys Arg Val Lys Ala Lys Ala 
            340                 345                 350         
Val Pro Ser 
        355 

<210> 4
<211> 355
<212> PRT
<213> Homo sapiens


<220> 
<223> SIRT6 N308K A313S variant amino acid sequence

<400> 4
Met Ser Val Asn Tyr Ala Ala Gly Leu Ser Pro Tyr Ala Asp Lys Gly 
1               5                   10                  15      
Lys Cys Gly Leu Pro Glu Ile Phe Asp Pro Pro Glu Glu Leu Glu Arg 
            20                  25                  30          
Lys Val Trp Glu Leu Ala Arg Leu Val Trp Gln Ser Ser Ser Val Val 
        35                  40                  45              
Phe His Thr Gly Ala Gly Ile Ser Thr Ala Ser Gly Ile Pro Asp Phe 
    50                  55                  60                  
Arg Gly Pro His Gly Val Trp Thr Met Glu Glu Arg Gly Leu Ala Pro 
65                  70                  75                  80  
Lys Phe Asp Thr Thr Phe Glu Ser Ala Arg Pro Thr Gln Thr His Met 
                85                  90                  95      
Ala Leu Val Gln Leu Glu Arg Val Gly Leu Leu Arg Phe Leu Val Ser 
            100                 105                 110         
Gln Asn Val Asp Gly Leu His Val Arg Ser Gly Phe Pro Arg Asp Lys 
        115                 120                 125             
Leu Ala Glu Leu His Gly Asn Met Phe Val Glu Glu Cys Ala Lys Cys 
    130                 135                 140                 
Lys Thr Gln Tyr Val Arg Asp Thr Val Val Gly Thr Met Gly Leu Lys 
145                 150                 155                 160 
Ala Thr Gly Arg Leu Cys Thr Val Ala Lys Ala Arg Gly Leu Arg Ala 
                165                 170                 175     
Cys Arg Gly Glu Leu Arg Asp Thr Ile Leu Asp Trp Glu Asp Ser Leu 
            180                 185                 190         
Pro Asp Arg Asp Leu Ala Leu Ala Asp Glu Ala Ser Arg Asn Ala Asp 
        195                 200                 205             
Leu Ser Ile Thr Leu Gly Thr Ser Leu Gln Ile Arg Pro Ser Gly Asn 
    210                 215                 220                 
Leu Pro Leu Ala Thr Lys Arg Arg Gly Gly Arg Leu Val Ile Val Asn 
225                 230                 235                 240 
Leu Gln Pro Thr Lys His Asp Arg His Ala Asp Leu Arg Ile His Gly 
                245                 250                 255     
Tyr Val Asp Glu Val Met Thr Arg Leu Met Lys His Leu Gly Leu Glu 
            260                 265                 270         
Ile Pro Ala Trp Asp Gly Pro Arg Val Leu Glu Arg Ala Leu Pro Pro 
        275                 280                 285             
Leu Pro Arg Pro Pro Thr Pro Lys Leu Glu Pro Lys Glu Glu Ser Pro 
    290                 295                 300                 
Thr Arg Ile Lys Gly Ser Ile Pro Ser Gly Pro Lys Gln Glu Pro Cys 
305                 310                 315                 320 
Ala Gln His Asn Gly Ser Glu Pro Ala Ser Pro Lys Arg Glu Arg Pro 
                325                 330                 335     
Thr Ser Pro Ala Pro His Arg Pro Pro Lys Arg Val Lys Ala Lys Ala 
            340                 345                 350         
Val Pro Ser 
        355 

<210> 5
<211> 1068
<212> DNA
<213> Homo sapiens


<220> 
<223> Wild type SIRT6 nucleic acid sequence

<400> 5
atgtcggtga attacgcggc ggggctgtcg ccgtacgcgg acaagggcaa gtgcggcctc      60

ccggagatct tcgacccccc ggaggagctg gagcggaagg tgtgggaact ggcgaggctg     120

gtctggcagt cttccagtgt ggtgttccac acgggtgccg gcatcagcac tgcctctggc     180

atccccgact tcaggggtcc ccacggagtc tggaccatgg aggagcgagg tctggccccc     240

aagttcgaca ccacctttga gagcgcgcgg cccacgcaga cccacatggc gctggtgcag     300

ctggagcgcg tgggcctcct ccgcttcctg gtcagccaga acgtggacgg gctccatgtg     360

cgctcaggct tccccaggga caaactggca gagctccacg ggaacatgtt tgtggaagaa     420

tgtgccaagt gtaagacgca gtacgtccga gacacagtcg tgggcaccat gggcctgaag     480

gccacgggcc ggctctgcac cgtggctaag gcaagggggc tgcgagcctg caggggagag     540

ctgagggaca ccatcctaga ctgggaggac tccctgcccg accgggacct ggcactcgcc     600

gatgaggcca gcaggaacgc cgacctgtcc atcacgctgg gtacatcgct gcagatccgg     660

cccagcggga acctgccgct ggctaccaag cgccggggag gccgcctggt catcgtcaac     720

ctgcagccca ccaagcacga ccgccatgct gacctccgca tccatggcta cgttgacgag     780

gtcatgaccc ggctcatgaa gcacctgggg ctggagatcc ccgcctggga cggcccccgt     840

gtgctggaga gggcgctgcc acccctgccc cgcccgccca cccccaagct ggagcccaag     900

gaggaatctc ccacccggat caacggctct atccccgccg gccccaagca ggagccctgc     960

gcccagcaca acggctcaga gcccgccagc cccaaacggg agcggcccac cagccctgcc    1020

ccccacagac cccccaaaag ggtgaaggcc aaggcggtcc ccagctga                 1068


<210> 6
<211> 1068
<212> DNA
<213> Artificial Sequence


<220> 
<223> SIRT6 N308K variant nucleic acid sequence

<400> 6
atgtcggtga attacgcggc ggggctgtcg ccgtacgcgg acaagggcaa gtgcggcctc      60

ccggagatct tcgacccccc ggaggagctg gagcggaagg tgtgggaact ggcgaggctg     120

gtctggcagt cttccagtgt ggtgttccac acgggtgccg gcatcagcac tgcctctggc     180

atccccgact tcaggggtcc ccacggagtc tggaccatgg aggagcgagg tctggccccc     240

aagttcgaca ccacctttga gagcgcgcgg cccacgcaga cccacatggc gctggtgcag     300

ctggagcgcg tgggcctcct ccgcttcctg gtcagccaga acgtggacgg gctccatgtg     360

cgctcaggct tccccaggga caaactggca gagctccacg ggaacatgtt tgtggaagaa     420

tgtgccaagt gtaagacgca gtacgtccga gacacagtcg tgggcaccat gggcctgaag     480

gccacgggcc ggctctgcac cgtggctaag gcaagggggc tgcgagcctg caggggagag     540

ctgagggaca ccatcctaga ctgggaggac tccctgcccg accgggacct ggcactcgcc     600

gatgaggcca gcaggaacgc cgacctgtcc atcacgctgg gtacatcgct gcagatccgg     660

cccagcggga acctgccgct ggctaccaag cgccggggag gccgcctggt catcgtcaac     720

ctgcagccca ccaagcacga ccgccatgct gacctccgca tccatggcta cgttgacgag     780

gtcatgaccc ggctcatgaa gcacctgggg ctggagatcc ccgcctggga cggcccccgt     840

gtgctggaga gggcgctgcc acccctgccc cgcccgccca cccccaagct ggagcccaag     900

gaggaatctc ccacccggat caagggctct atccccgccg gccccaagca ggagccctgc     960

gcccagcaca acggctcaga gcccgccagc cccaaacggg agcggcccac cagccctgcc    1020

ccccacagac cccccaaaag ggtgaaggcc aaggcggtcc ccagctga                 1068


<210> 7
<211> 1068
<212> DNA
<213> Artificial Sequence


<220> 
<223> SIRT6 A313S variant nucleic acid sequence

<400> 7
atgtcggtga attacgcggc ggggctgtcg ccgtacgcgg acaagggcaa gtgcggcctc      60

ccggagatct tcgacccccc ggaggagctg gagcggaagg tgtgggaact ggcgaggctg     120

gtctggcagt cttccagtgt ggtgttccac acgggtgccg gcatcagcac tgcctctggc     180

atccccgact tcaggggtcc ccacggagtc tggaccatgg aggagcgagg tctggccccc     240

aagttcgaca ccacctttga gagcgcgcgg cccacgcaga cccacatggc gctggtgcag     300

ctggagcgcg tgggcctcct ccgcttcctg gtcagccaga acgtggacgg gctccatgtg     360

cgctcaggct tccccaggga caaactggca gagctccacg ggaacatgtt tgtggaagaa     420

tgtgccaagt gtaagacgca gtacgtccga gacacagtcg tgggcaccat gggcctgaag     480

gccacgggcc ggctctgcac cgtggctaag gcaagggggc tgcgagcctg caggggagag     540

ctgagggaca ccatcctaga ctgggaggac tccctgcccg accgggacct ggcactcgcc     600

gatgaggcca gcaggaacgc cgacctgtcc atcacgctgg gtacatcgct gcagatccgg     660

cccagcggga acctgccgct ggctaccaag cgccggggag gccgcctggt catcgtcaac     720

ctgcagccca ccaagcacga ccgccatgct gacctccgca tccatggcta cgttgacgag     780

gtcatgaccc ggctcatgaa gcacctgggg ctggagatcc ccgcctggga cggcccccgt     840

gtgctggaga gggcgctgcc acccctgccc cgcccgccca cccccaagct ggagcccaag     900

gaggaatctc ccacccggat caacggctct atcccctccg gccccaagca ggagccctgc     960

gcccagcaca acggctcaga gcccgccagc cccaaacggg agcggcccac cagccctgcc    1020

ccccacagac cccccaaaag ggtgaaggcc aaggcggtcc ccagctga                 1068


<210> 8
<211> 1068
<212> DNA
<213> Homo sapiens


<220> 
<223> SIRT6 N308K A313S variant nucleic acid sequence

<400> 8
atgtcggtga attacgcggc ggggctgtcg ccgtacgcgg acaagggcaa gtgcggcctc      60

ccggagatct tcgacccccc ggaggagctg gagcggaagg tgtgggaact ggcgaggctg     120

gtctggcagt cttccagtgt ggtgttccac acgggtgccg gcatcagcac tgcctctggc     180

atccccgact tcaggggtcc ccacggagtc tggaccatgg aggagcgagg tctggccccc     240

aagttcgaca ccacctttga gagcgcgcgg cccacgcaga cccacatggc gctggtgcag     300

ctggagcgcg tgggcctcct ccgcttcctg gtcagccaga acgtggacgg gctccatgtg     360

cgctcaggct tccccaggga caaactggca gagctccacg ggaacatgtt tgtggaagaa     420

tgtgccaagt gtaagacgca gtacgtccga gacacagtcg tgggcaccat gggcctgaag     480

gccacgggcc ggctctgcac cgtggctaag gcaagggggc tgcgagcctg caggggagag     540

ctgagggaca ccatcctaga ctgggaggac tccctgcccg accgggacct ggcactcgcc     600

gatgaggcca gcaggaacgc cgacctgtcc atcacgctgg gtacatcgct gcagatccgg     660

cccagcggga acctgccgct ggctaccaag cgccggggag gccgcctggt catcgtcaac     720

ctgcagccca ccaagcacga ccgccatgct gacctccgca tccatggcta cgttgacgag     780

gtcatgaccc ggctcatgaa gcacctgggg ctggagatcc ccgcctggga cggcccccgt     840

gtgctggaga gggcgctgcc acccctgccc cgcccgccca cccccaagct ggagcccaag     900

gaggaatctc ccacccggat caagggctct atcccctccg gccccaagca ggagccctgc     960

gcccagcaca acggctcaga gcccgccagc cccaaacggg agcggcccac cagccctgcc    1020

ccccacagac cccccaaaag ggtgaaggcc aaggcggtcc ccagctga                 1068


<210> 9
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> LINE1 ORF1 Forward-primer

<400> 9
atggcgaaag gcaaacgtaa g                                              21


<210> 10
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> LINE1 ORF1 Reverse-primer

<400> 10
attttcggtt gtgttggggt g                                              21


<210> 11
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> LINE1 ORF2 Forward-primer

<400> 11
gcaggggttg caatcctagt c                                              21


<210> 12
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> LINE1 ORF2 Reverse-primer

<400> 12
ctgggtgctc ctgtattggg t                                              21


<210> 13
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> N1SMA forward primer

<400> 13
aaaagacagc tacgtgggtg a                                              21


<210> 14
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> N1SMA reverse primer

<400> 14
gccatgttct atcgggtact tc                                             22


<210> 15
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> COL1A1 forward primer

<400> 15
gtgcgatgac gtgatctgtg a                                              21


<210> 16
<211> 19
<212> DNA
<213> Artificial Sequence


<220> 
<223> COL1A1 reverse primer

<400> 16
cggtggtttc ttggtcggt                                                 19


<210> 17
<211> 23
<212> DNA
<213> Artificial Sequence


<220> 
<223> TIMP1 forward primer

<400> 17
accaccttat accagcgtta tga                                            23


<210> 18
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> TIMP1 reverse primer

<400> 18
ggtgtagacg aaccggatgt c                                              21


<210> 19
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> VIMENTIN forward primer

<400> 19
agtccactga gtaccggaga c                                              21


<210> 20
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> VIMENTIN reverse primer

<400> 20
catttcacgc atctggcgtt c                                              21


<210> 21
<211> 23
<212> DNA
<213> Artificial Sequence


<220> 
<223> MMP2 forward primer

<400> 21
tacaggatca ttggctacac acc                                            23


<210> 22
<211> 19
<212> DNA
<213> Artificial Sequence


<220> 
<223> MMP2 reverse primer

<400> 22
ggtcacatcg ctccagact                                                 19


<210> 23
<211> 17
<212> DNA
<213> Artificial Sequence


<220> 
<223> GAPDH forward primer

<400> 23
ggtgcgtgcc cagttga                                                   17


<210> 24
<211> 19
<212> DNA
<213> Artificial Sequence


<220> 
<223> GAPDH reverse primer

<400> 24
tactttctcc ccgcttttt                                                 19


<210> 25
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> ActinN2 forward primer

<400> 25
catgtacgtt gctatccagg c                                              21


<210> 26
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> ActinN2 reverse primer

<400> 26
ctccttaatg tcacgcacga t                                              21


