                         SEQUENCE LISTING

<110>  Arizona Board of Regents on behalf of Arizona State 
       University
       Chen, Qiang
       Yang, Ming
       Sun, Haiyan
       Lai, Huafang
 
<120>  A PLANT PRODUCED ANTI-EGFR MABS WITH SPECIFIC GLYCOSYLATION TO 
       IMPROVE THE EFFICACY AGAINST CANCER

<130>  112624.01330

<150>  63/188,360
<151>  2021-05-13

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- Heavy chain variable domain (VH)

<400>  1
caagtgcaat tgaaacagtc cggcccagga ttggtccagc catcgcaaag tctctctatt       60

acatgtaccg ttagcggttt ttctcttact aactatgggg ttcattgggt cagacagtca      120

cctgggaaag gattagagtg gcttggcgtt atctggtctg gtggaaatac tgactacaat      180

actcccttta catcaaggct atccattaac aaggataact ctaagagcca agtgttcttc      240

aagatgaata gtctgcagag taatgatacg gctatatact attgcgcacg tgccttgact      300

tactatgatt atgaatttgc ttactggggt caaggaacac ttgtaaccgt atcagca         357


<210>  2
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- Heavy chain variable domain (VH)

<400>  2

Gln Val Gln Leu Lys Gln Ser Gly Pro Gly Leu Val Gln Pro Ser Gln 
1               5                   10                  15      


Ser Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu Thr Asn Tyr 
            20                  25                  30          


Gly Val His Trp Val Arg Gln Ser Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Gly Val Ile Trp Ser Gly Gly Asn Thr Asp Tyr Asn Thr Pro Phe Thr 
    50                  55                  60                  


Ser Arg Leu Ser Ile Asn Lys Asp Asn Ser Lys Ser Gln Val Phe Phe 
65                  70                  75                  80  


Lys Met Asn Ser Leu Gln Ser Asn Asp Thr Ala Ile Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Ala Leu Thr Tyr Tyr Asp Tyr Glu Phe Ala Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ala 
        115                 


<210>  3
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- Light chain variable domain (VL)

<400>  3
gatattctcc taacgcaaag tcccgtaatt ctgagtgtct cccctgggga aagggtgtcg       60

tttagctgtc gagcttctca atccattggt acaaacatac attggtacca gcagagaact      120

aatggttctc ctcgtttatt gatcaaatat gcatctgagt caatttctgg cataccatca      180

agattctctg gaagcggttc aggaactgac tttacccttt caatcaatag tgttgaatcc      240

gaggatattg ccgattacta ttgccagcaa aacaacaatt ggccaacaac tttcggagct      300

ggcacaaagt tggaacttaa a                                                321


<210>  4
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- Light chain variable domain (VL)

<400>  4

Asp Ile Leu Leu Thr Gln Ser Pro Val Ile Leu Ser Val Ser Pro Gly 
1               5                   10                  15      


Glu Arg Val Ser Phe Ser Cys Arg Ala Ser Gln Ser Ile Gly Thr Asn 
            20                  25                  30          


Ile His Trp Tyr Gln Gln Arg Thr Asn Gly Ser Pro Arg Leu Leu Ile 
        35                  40                  45              


Lys Tyr Ala Ser Glu Ser Ile Ser Gly Ile Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Ser Ile Asn Ser Val Glu Ser 
65                  70                  75                  80  


Glu Asp Ile Ala Asp Tyr Tyr Cys Gln Gln Asn Asn Asn Trp Pro Thr 
                85                  90                  95      


Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105         


<210>  5
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic-Plant signal peptide

<400>  5
atgggatggt cttgtatcat ccttttcttg gttgcaacag ctactggtgt tcattct          57


<210>  6
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- Plant signal peptide

<400>  6

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser 
            


<210>  7
<211>  1452
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- Heavy chain, wild-type

<400>  7
gaattctttt ggtctcacta gacaatggga tggtcttgta tcatcctttt cttggttgca       60

acagctactg gtgttcattc tcaagtgcaa ttgaaacagt ccggcccagg attggtccag      120

ccatcgcaaa gtctctctat tacatgtacc gttagcggtt tttctcttac taactatggg      180

gttcattggg tcagacagtc acctgggaaa ggattagagt ggcttggcgt tatctggtct      240

ggtggaaata ctgactacaa tactcccttt acatcaaggc tatccattaa caaggataac      300

tctaagagcc aagtgttctt caagatgaat agtctgcaga gtaatgatac ggctatatac      360

tattgcgcac gtgccttgac ttactatgat tatgaatttg cttactgggg tcaaggaaca      420

cttgtaaccg tatcagcagc tagcaccaag ggaccttctg tttttccact tgctccttct      480

tctaagtcta cttctggtgg aactgctgct ttgggttgtt tggtgaaaga ttactttcct      540

gagccagtga ccgtttcttg gaactcaggt gctcttacat ctggtgttca tactttccca      600

gctgttcttc aatcttcagg actttactca ctttcttctg ttgttaccgt tccttcttca      660

agcttgggca ctcagaccta catctgcaat gtgaatcaca aacccagcaa caccaaggtt      720

gacaagaaag ttgagcccaa gtcttgtgac aagactcata cgtgtccacc gtgcccagca      780

cctgaacttc ttggaggacc gtcagtcttc ttgtttcctc caaagcctaa ggataccttg      840

atgatctcca ggactcctga agtcacatgt gtagttgtgg atgtgagcca tgaagatcct      900

gaggtgaagt tcaactggta tgtggatggt gtggaagtgc acaatgccaa gacaaagccg      960

agagaggaac agtacaacag cacgtacagg gttgtctcag ttctcactgt tctccatcaa     1020

gattggttga atggcaaaga gtacaagtgc aaggtgtcca acaaagccct cccagccccc     1080

attgagaaga ccatttccaa agcgaaaggg caaccccgtg aaccacaagt gtacacactt     1140

cctccatctc gcgatgaact gaccaagaac caggtcagct tgacttgcct ggtgaaaggc     1200

ttctatccct ctgacatagc tgtagagtgg gagagcaatg ggcaaccgga gaacaactac     1260

aagactacac ctcccgttct cgattctgac ggctccttct tcctctacag caagctcaca     1320

gtggacaaga gcaggtggca acaagggaat gtcttctcat gctccgtgat gcatgaggct     1380

cttcacaatc actacacaca gaagagtctc tccttgtctc cgggtaaatg aagctagaga     1440

ccaaaaggat cc                                                         1452


<210>  8
<211>  468
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- Heavy chain, wild-type

<400>  8

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Gln Val Gln Leu Lys Gln Ser Gly Pro Gly Leu Val Gln 
            20                  25                  30          


Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu 
        35                  40                  45              


Thr Asn Tyr Gly Val His Trp Val Arg Gln Ser Pro Gly Lys Gly Leu 
    50                  55                  60                  


Glu Trp Leu Gly Val Ile Trp Ser Gly Gly Asn Thr Asp Tyr Asn Thr 
65                  70                  75                  80  


Pro Phe Thr Ser Arg Leu Ser Ile Asn Lys Asp Asn Ser Lys Ser Gln 
                85                  90                  95      


Val Phe Phe Lys Met Asn Ser Leu Gln Ser Asn Asp Thr Ala Ile Tyr 
            100                 105                 110         


Tyr Cys Ala Arg Ala Leu Thr Tyr Tyr Asp Tyr Glu Phe Ala Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Leu Val Thr Val Ser Ala Ala Ser Thr Lys Gly Pro 
    130                 135                 140                 


Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr 
145                 150                 155                 160 


Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr 
                165                 170                 175     


Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro 
            180                 185                 190         


Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr 
        195                 200                 205             


Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn 
    210                 215                 220                 


His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser 
225                 230                 235                 240 


Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu 
                245                 250                 255     


Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
            260                 265                 270         


Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
        275                 280                 285             


His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu 
    290                 295                 300                 


Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr 
305                 310                 315                 320 


Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 
                325                 330                 335     


Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro 
            340                 345                 350         


Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln 
        355                 360                 365             


Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val 
    370                 375                 380                 


Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
385                 390                 395                 400 


Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
                405                 410                 415     


Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 
            420                 425                 430         


Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 
        435                 440                 445             


Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
    450                 455                 460                 


Ser Pro Gly Lys 
465             


<210>  9
<211>  1452
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- Heavy chain, E430G mutant


<220>
<221>  misc_feature
<222>  (1375)..(1377)
<223>  GAG to GGT mutation

<400>  9
gaattctttt ggtctcacta gacaatggga tggtcttgta tcatcctttt cttggttgca       60

acagctactg gtgttcattc tcaagtgcaa ttgaaacagt ccggcccagg attggtccag      120

ccatcgcaaa gtctctctat tacatgtacc gttagcggtt tttctcttac taactatggg      180

gttcattggg tcagacagtc acctgggaaa ggattagagt ggcttggcgt tatctggtct      240

ggtggaaata ctgactacaa tactcccttt acatcaaggc tatccattaa caaggataac      300

tctaagagcc aagtgttctt caagatgaat agtctgcaga gtaatgatac ggctatatac      360

tattgcgcac gtgccttgac ttactatgat tatgaatttg cttactgggg tcaaggaaca      420

cttgtaaccg tatcagcagc tagcaccaag ggaccttctg tttttccact tgctccttct      480

tctaagtcta cttctggtgg aactgctgct ttgggttgtt tggtgaaaga ttactttcct      540

gagccagtga ccgtttcttg gaactcaggt gctcttacat ctggtgttca tactttccca      600

gctgttcttc aatcttcagg actttactca ctttcttctg ttgttaccgt tccttcttca      660

agcttgggca ctcagaccta catctgcaat gtgaatcaca aacccagcaa caccaaggtt      720

gacaagaaag ttgagcccaa gtcttgtgac aagactcata cgtgtccacc gtgcccagca      780

cctgaacttc ttggaggacc gtcagtcttc ttgtttcctc caaagcctaa ggataccttg      840

atgatctcca ggactcctga agtcacatgt gtagttgtgg atgtgagcca tgaagatcct      900

gaggtgaagt tcaactggta tgtggatggt gtggaagtgc acaatgccaa gacaaagccg      960

agagaggaac agtacaacag cacgtacagg gttgtctcag ttctcactgt tctccatcaa     1020

gattggttga atggcaaaga gtacaagtgc aaggtgtcca acaaagccct cccagccccc     1080

attgagaaga ccatttccaa agcgaaaggg caaccccgtg aaccacaagt gtacacactt     1140

cctccatctc gcgatgaact gaccaagaac caggtcagct tgacttgcct ggtgaaaggc     1200

ttctatccct ctgacatagc tgtagagtgg gagagcaatg ggcaaccgga gaacaactac     1260

aagactacac ctcccgttct cgattctgac ggctccttct tcctctacag caagctcaca     1320

gtggacaaga gcaggtggca acaagggaat gtcttctcat gctccgtgat gcatggtgct     1380

cttcacaatc actacacaca gaagagtctc tccttgtctc cgggtaaatg aagctagaga     1440

ccaaaaggat cc                                                         1452


<210>  10
<211>  468
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- Heavy chain, E430G mutant


<220>
<221>  MISC_FEATURE
<222>  (451)..(451)
<223>  Mutation from E to G

<400>  10

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Gln Val Gln Leu Lys Gln Ser Gly Pro Gly Leu Val Gln 
            20                  25                  30          


Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu 
        35                  40                  45              


Thr Asn Tyr Gly Val His Trp Val Arg Gln Ser Pro Gly Lys Gly Leu 
    50                  55                  60                  


Glu Trp Leu Gly Val Ile Trp Ser Gly Gly Asn Thr Asp Tyr Asn Thr 
65                  70                  75                  80  


Pro Phe Thr Ser Arg Leu Ser Ile Asn Lys Asp Asn Ser Lys Ser Gln 
                85                  90                  95      


Val Phe Phe Lys Met Asn Ser Leu Gln Ser Asn Asp Thr Ala Ile Tyr 
            100                 105                 110         


Tyr Cys Ala Arg Ala Leu Thr Tyr Tyr Asp Tyr Glu Phe Ala Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Leu Val Thr Val Ser Ala Ala Ser Thr Lys Gly Pro 
    130                 135                 140                 


Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr 
145                 150                 155                 160 


Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr 
                165                 170                 175     


Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro 
            180                 185                 190         


Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr 
        195                 200                 205             


Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn 
    210                 215                 220                 


His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser 
225                 230                 235                 240 


Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu 
                245                 250                 255     


Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
            260                 265                 270         


Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
        275                 280                 285             


His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu 
    290                 295                 300                 


Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr 
305                 310                 315                 320 


Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 
                325                 330                 335     


Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro 
            340                 345                 350         


Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln 
        355                 360                 365             


Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val 
    370                 375                 380                 


Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
385                 390                 395                 400 


Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
                405                 410                 415     


Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 
            420                 425                 430         


Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 
        435                 440                 445             


Met His Gly Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
    450                 455                 460                 


Ser Pro Gly Lys 
465             


<210>  11
<211>  732
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- Light chain

<400>  11
gaattctcta gaacaatggg atggtcttgt atcatccttt tcttggttgc aacagctact       60

ggtgttcatt ctgatattct cctaacgcaa agtcccgtaa ttctgagtgt ctcccctggg      120

gaaagggtgt cgtttagctg tcgagcttct caatccattg gtacaaacat acattggtac      180

cagcagagaa ctaatggttc tcctcgttta ttgatcaaat atgcatctga gtcaatttct      240

ggcataccat caagattctc tggaagcggt tcaggaactg actttaccct ttcaatcaat      300

agtgttgaat ccgaggatat tgccgattac tattgccagc aaaacaacaa ttggccaaca      360

actttcggag ctggcacaaa gttggaactt aaagctagca gaactgttgc tgcaccatct      420

gttttcatct tccctccatc tgatgagcag ttgaaatctg gaactgcttc tgttgtgtgc      480

cttcttaata acttctatcc tagagaggct aaagttcagt ggaaggtgga taacgcactt      540

caatctggta actctcaaga gtctgttaca gagcaagatt ctaaggactc aacttactct      600

ctttcatcta cacttacttt gtcaaaagca gattacgaga aacacaaagt ttacgcatgc      660

gaagttactc atcaaggact ttcttcacca gttacaaagt ctttcaatag aggagagtgt      720

taatgagagc tc                                                          732


<210>  12
<211>  235
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- Light chain


<220>
<221>  MISC_FEATURE
<222>  (127)..(128)
<223>  AS Linker

<400>  12

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Asp Ile Leu Leu Thr Gln Ser Pro Val Ile Leu Ser Val 
            20                  25                  30          


Ser Pro Gly Glu Arg Val Ser Phe Ser Cys Arg Ala Ser Gln Ser Ile 
        35                  40                  45              


Gly Thr Asn Ile His Trp Tyr Gln Gln Arg Thr Asn Gly Ser Pro Arg 
    50                  55                  60                  


Leu Leu Ile Lys Tyr Ala Ser Glu Ser Ile Ser Gly Ile Pro Ser Arg 
65                  70                  75                  80  


Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Ser Ile Asn Ser 
                85                  90                  95      


Val Glu Ser Glu Asp Ile Ala Asp Tyr Tyr Cys Gln Gln Asn Asn Asn 
            100                 105                 110         


Trp Pro Thr Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys Ala Ser 
        115                 120                 125             


Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
    130                 135                 140                 


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
145                 150                 155                 160 


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
                165                 170                 175     


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
            180                 185                 190         


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
        195                 200                 205             


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
    210                 215                 220                 


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
225                 230                 235 


