                         SEQUENCE LISTING

<110>  Becton, Dickinson and Company
       Godinez, Alvaro
       Fulcher, Robert Aubrey
 
<120>  MULTIPLEXED UNBIASED NUCLEIC ACID AMPLIFICATION METHOD

<130>  68EB-317327-WO

<150>  US 63/189,021
<151>  2021-05-14

<150>  US 63/243,449
<151>  2021-09-13

<160>  45    

<170>  PatentIn version 3.5

<210>  1
<211>  12339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3XFlag-Cas9-Fl26-Tn5

<400>  1
atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc       60

cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac      120

cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca      180

gctgcggtaa agctcatcag cgtggtcgtg cagcgattca cagatgtctg cctgttcatc      240

cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc      300

catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct      360

gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga      420

tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg      480

gcgggaccag agaaaaatca ctcagggtca atgccagccg aacgccagca agacgtagcc      540

cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc      600

gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag      660

gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc      720

cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt      780

catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg      840

agatcccggt gcctaatgag tgagctaact tacattaatt gcgttgcgct cactgcccgc      900

tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag      960

aggcggtttg cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca     1020

gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt     1080

gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt     1140

cttcggtatc gtcgtatccc actaccgaga tatccgcacc aacgcgcagc ccggactcgg     1200

taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa     1260

cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc     1320

cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca     1380

gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac     1440

ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac     1500

tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag     1560

cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc     1620

gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca     1680

tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt     1740

gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc     1800

ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca     1860

ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct     1920

gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca     1980

ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt     2040

cgatggtgtc cgggatctcg acgctctccc ttatgcgact cctgcattag gaagcagccc     2100

agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg ccggcatgcc     2160

gccctttcgt cttcaagaat taattcccaa ttccccaggc atcaaataaa acgaaaggct     2220

cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt     2280

aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg     2340

gcaggacgcc cgccataaac tgccaggaat taattcccca ggcatcaaat aaaacgaaag     2400

gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg     2460

agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg     2520

cgggcaggac gcccgccata aactgccagg aattaattcc ccaggcatca aataaaacga     2580

aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc     2640

ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg     2700

tggcgggcag gacgcccgcc ataaactgcc aggaattaat tccccaggca tcaaataaaa     2760

cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct     2820

ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga     2880

gggtggcggg caggacgccc gccataaact gccaggaatt aattccccag gcatcaaata     2940

aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac     3000

gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc     3060

ggagggtggc gggcaggacg cccgccataa actgccagga attggggatc ggaattaatt     3120

cccggtttaa accggggatc tcgatcccgc gaaattaata cgactcacta taggggaatt     3180

gtgagcggat aacaattccc ctctagaaat aattttgttt aactttaaga aggagatata     3240

ccatgggtga ttacaaggat cacgatggcg attacaagga tcacgatatc gattacaagg     3300

atgatgatga taagatggat aaaaagtatt ctattggttt agctatcggc acaaatagcg     3360

tcggatgggc ggtgatcact gatgaatata aggttccgtc taaaaagttc aaggttctgg     3420

gaaatacaga ccgccacagt atcaaaaaaa atcttatagg ggctctttta tttgacagtg     3480

gagagacagc ggaagcgact cgtctcaaac ggacagctcg tagaaggtat acacgtcgga     3540

agaatcgtat ttgttatcta caggagattt tttcaaatga gatggcgaaa gtagatgata     3600

gtttctttca tcgacttgaa gagtcttttt tggtggaaga agacaagaag catgaacgtc     3660

atcctatttt tggaaatata gtagatgaag ttgcttatca tgagaaatat ccaactatct     3720

atcatctgcg aaaaaaattg gtagattcta ctgataaagc ggatttgcgc ttaatctatt     3780

tggccttagc gcatatgatt aagtttcgtg gtcatttttt gattgaggga gatttaaatc     3840

ctgataatag tgatgtggac aaactattta tccagttggt acaaacctac aatcaattat     3900

ttgaagaaaa ccctattaac gcaagtggag tagatgctaa agcgattctt tctgcacgat     3960

tgagtaaatc aagacgatta gaaaatctca ttgctcagct ccccggtgag aagaaaaatg     4020

gcttatttgg gaatctcatt gctttgtcat tgggtttgac ccctaatttt aaatcaaatt     4080

ttgatttggc agaagatgct aaattacagc tttcaaaaga tacttacgat gatgatttag     4140

ataatttatt ggcgcaaatt ggagatcaat atgctgattt gtttttggca gctaagaatt     4200

tatcagatgc tattttactt tcagatatcc taagagtaaa tactgaaata actaaggctc     4260

ccctatcagc atcaatgatt aaacgctacg atgaacatca tcaagacttg actcttttaa     4320

aagctttagt tcgacaacaa cttccagaaa agtataaaga aatctttttt gatcaatcaa     4380

aaaacggata tgcaggttat attgatgggg gagctagcca agaagaattt tataaattta     4440

tcaaaccaat tttagaaaaa atggatggta ctgaggaatt attggtgaaa ctaaatcgtg     4500

aagatttgct gcgcaagcaa cggacctttg acaacggctc tattccccat caaattcact     4560

tgggtgagct gcatgctatt ttgagaagac aagaagactt ttatccattt ttaaaagaca     4620

atcgtgagaa gattgaaaaa atcttgactt ttcgaattcc ttattatgtt ggtccattgg     4680

cgcgtggcaa tagtcgtttt gcatggatga ctcggaagtc tgaagaaaca attaccccat     4740

ggaattttga agaagttgtc gataaaggtg cttcagctca atcatttatt gaacgcatga     4800

caaactttga taaaaatctt ccaaatgaaa aagtactacc aaaacatagt ttgctttatg     4860

agtattttac ggtttataac gaattgacaa aggtcaaata tgttactgaa ggaatgcgaa     4920

aaccagcatt tctttcaggt gaacagaaga aagccattgt tgatttactc ttcaaaacaa     4980

atcgaaaagt aaccgttaag caattaaaag aagattattt caaaaaaata gaatgttttg     5040

atagtgttga aatttcagga gttgaagata gatttaatgc ttcattaggt acctaccatg     5100

atttgctaaa aattattaaa gataaagatt ttttggataa tgaagaaaat gaagatatct     5160

tagaggatat tgttttaaca ttgaccttat ttgaagatag ggagatgatt gaggaaagac     5220

ttaaaacata tgctcacctc tttgatgata aggtgatgaa acagcttaaa cgtcgccgtt     5280

atactggttg gggacgtttg tctcgaaaat tgattaatgg tattagggat aagcaatctg     5340

gcaaaacaat attagatttt ttgaaatcag atggttttgc caatcgcaat tttatgcagc     5400

tgatccatga tgatagtttg acatttaaag aagacattca aaaagcacaa gtgtctggac     5460

aaggcgatag tttacatgaa catattgcaa atttagctgg tagccctgct attaaaaaag     5520

gtattttaca gactgtaaaa gttgttgatg aattggtcaa agtaatgggg cggcataagc     5580

cagaaaatat cgttattgaa atggcacgtg aaaatcagac aactcaaaag ggccagaaaa     5640

attcgcgaga gcgtatgaaa cgaatcgaag aaggtatcaa agaattagga agtcagattc     5700

ttaaagagca tcctgttgaa aatactcaat tgcaaaatga aaagctctat ctctattatc     5760

tccaaaatgg aagagacatg tatgtggacc aagaattaga tattaatcgt ttaagtgatt     5820

atgatgtcga tgccattgtt ccacaaagtt tccttaaaga cgattcaata gacaataagg     5880

tcttaacgcg ttctgataaa aatcgtggta aatcggataa cgttccaagt gaagaagtag     5940

tcaaaaagat gaaaaactat tggagacaac ttctaaacgc caagttaatc actcaacgta     6000

agtttgataa tttaacgaaa gctgaacgtg gaggtttgag tgaacttgat aaagctggtt     6060

ttatcaaacg ccaattggtt gaaactcgcc aaatcactaa gcatgtggca caaattttgg     6120

atagtcgcat gaatactaaa tacgatgaaa atgataaact tattcgagag gttaaagtga     6180

ttaccttaaa atctaaatta gtttctgact tccgaaaaga tttccaattc tataaagtac     6240

gtgagattaa caattaccat catgcccatg atgcgtatct aaatgccgtc gttggaactg     6300

ctttgattaa gaaatatcca aaacttgaat cggagtttgt ctatggtgat tataaagttt     6360

atgatgttcg taaaatgatt gctaagtctg agcaagaaat aggcaaagca accgcaaaat     6420

atttctttta ctctaatatc atgaacttct tcaaaacaga aattacactt gcaaatggag     6480

agattcgcaa acgccctcta atcgaaacta atggggaaac tggagaaatt gtctgggata     6540

aagggcgaga ttttgccaca gtgcgcaaag tattgtccat gccccaagtc aatattgtca     6600

agaaaacaga agtacagaca ggcggattct ccaaggagtc aattttacca aaaagaaatt     6660

cggacaagct tattgctcgt aaaaaagact gggatccaaa aaaatatggt ggttttgata     6720

gtccaacggt agcttattca gtcctagtgg ttgctaaggt ggaaaaaggg aaatcgaaga     6780

agttaaaatc cgttaaagag ttactaggga tcacaattat ggaaagaagt tcctttgaaa     6840

aaaatccgat tgacttttta gaagctaaag gatataagga agttaaaaaa gacttaatca     6900

ttaaactacc taaatatagt ctttttgagt tagaaaacgg tcgtaaacgg atgctggcta     6960

gtgccggaga attacaaaaa ggaaatgagc tggctctgcc aagcaaatat gtgaattttt     7020

tatatttagc tagtcattat gaaaagttga agggtagtcc agaagataac gaacaaaaac     7080

aattgtttgt ggagcagcat aagcattatt tagatgagat tattgagcaa atcagtgaat     7140

tttctaagcg tgttatttta gcagatgcca atttagataa agttcttagt gcatataaca     7200

aacatagaga caaaccaata cgtgaacaag cagaaaatat tattcattta tttacgttga     7260

cgaatcttgg agctcccgct gcttttaaat attttgatac aacaattgat cgtaaacgat     7320

atacgtctac aaaagaagtt ttagatgcca ctcttatcca tcaatccatc actggtcttt     7380

atgaaacacg cattgatttg agtcagctag gaggtgacga tgacgataaa gaattcggtg     7440

gcggtggctc tggcggtggt gggagtggag gtgggggatc aggaggaggc ggttcccata     7500

tgattaccag tgcactgcat cgtgcggcgg attgggcgaa aagcgtgttt tctagtgctg     7560

cgctgggtga tccgcgtcgt accgcgcgtc tggtgaatgt tgcggcgcaa ctggccaaat     7620

atagcggcaa aagcattacc attagcagcg aaggcagcaa agccatgcag gaaggcgcgt     7680

atcgttttat tcgtaatccg aacgtgagcg cggaagcgat tcgtaaagcg ggtgccatgc     7740

agaccgtgaa actggcccag gaatttccgg aactgctggc aattgaagat accacctctc     7800

tgagctatcg tcatcaggtg gcggaagaac tgggcaaact gggtagcatt caggataaaa     7860

gccgtggttg gtgggtgcat agcgtgctgc tgctggaagc gaccaccttt cgtaccgtgg     7920

gcctgctgca tcaagaatgg tggatgcgtc cggatgatcc ggcggatgcg gatgaaaaag     7980

aaagcggcaa atggctggcc gctgctgcaa cttcgcgtct gagaatgggc agcatgatga     8040

gcaacgtgat tgcggtgtgc gatcgtgaag cggatattca tgcgtatctg caagataaac     8100

tggcccataa cgaacgtttt gtggtgcgta gcaaacatcc gcgtaaagat gtggaaagcg     8160

gcctgtatct gtatgatcac ctgaaaaacc agccggaact gggcggctat cagattagca     8220

ttccgcagaa aggcgtggtg gataaacgtg gcaaacgtaa aaaccgtccg gcgcgtaaag     8280

cgagcctgag cctgcgtagc ggccgtatta ccctgaaaca gggcaacatt accctgaacg     8340

cggtgctggc cgaagaaatt aatccgccga aaggcgaaac cccgctgaaa tggctgctgc     8400

tgaccagcga gccggtggaa agtctggccc aagcgctgcg tgtgattgat atttataccc     8460

atcgttggcg cattgaagaa tttcacaaag cgtggaaaac gggtgcgggt gcggaacgtc     8520

agcgtatgga agaaccggat aacctggaac gtatggtgag cattctgagc tttgtggcgg     8580

tgcgtctgct gcaactgcgt gaatctttta ctccgccgca agcactgcgt gcgcagggcc     8640

tgctgaaaga agcggaacac gttgaaagcc agagcgcgga aaccgtgctg accccggatg     8700

aatgccaact gctgggctat ctggataaag gcaaacgcaa acgcaaagaa aaagcgggca     8760

gcctgcaatg ggcgtatatg gcgattgcgc gtctgggcgg ctttatggat agcaaacgta     8820

ccggcattgc gagctggggt gcgctgtggg aaggttggga agcgctgcaa agcaaactgg     8880

atggctttct ggccgcgaaa gacctgatgg cgcagggcat taaaatctgc atcacgggag     8940

atgcactagt tgccctaccc gagggcgagt cggtacgcat cgccgacatc gtgccgggtg     9000

cgcggcccaa cagtgacaac gccatcgacc tgaaagtcct tgaccggcat ggcaatcccg     9060

tgctcgccga ccggctgttc cactccggcg agcatccggt gtacacggtg cgtacggtcg     9120

aaggtctgcg tgtgacgggc accgcgaacc acccgttgtt gtgtttggtc gacgtcgccg     9180

gggtgccgac cctgctgtgg aagctgatcg acgaaatcaa gccgggcgat tacgcggtga     9240

ttcaacgcag cgcattcagc gtcgactgtg caggttttgc ccgcgggaaa cccgaatttg     9300

cgcccacaac ctacacagtc ggcgtccctg gactggtgcg tttcttggaa gcacaccacc     9360

gagacccgga cgcccaagct atcgccgacg agctgaccga cgggcggttc tactacgcga     9420

aagtcgccag tgtcaccgac gccggcgtgc agccggtgta tagccttcgt gtcgacacgg     9480

cagaccacgc gtttatcacg aacgggttcg tcagccacgc tactggcctc accggtctga     9540

actcaggcct cacgacaaat cctggtgtat ccgcttggca ggtcaacaca gcttatactg     9600

cgggacaatt ggtcacatat aacggcaaga cgtataaatg tttgcagccc cacacctcct     9660

tggcaggatg ggaaccatcc aacgttcctg ccttgtggca gcttcaatga ctgcaggaag     9720

gggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc     9780

aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag     9840

gaggaactat atccggataa ctacgtcagg tggcactttt cggggaaatg tgcgcggaac     9900

ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc     9960

ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt    10020

cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct    10080

ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga    10140

tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgtttcc caatgatgag    10200

cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca    10260

actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga    10320

aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag    10380

tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc    10440

ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa    10500

tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt    10560

gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg    10620

gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt    10680

tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg    10740

gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat    10800

ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact    10860

gtcagaccaa gtttactcat atatacttta gattgattta ccccggttga taatcagaaa    10920

agccccaaaa acaggaagat tgtataagca aatatttaaa ttgtaaacgt taatattttg    10980

ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc    11040

ggcaaaatcc cttataaatc aaaagaatag cccgagatag ggttgagtgt tgttccagtt    11100

tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc    11160

tatcagggcg atggcccact acgtgaacca tcacccaaat caagtttttt ggggtcgagg    11220

tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga    11280

aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg    11340

ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg    11400

ctacagggcg cgtaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat    11460

cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc    11520

ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct    11580

accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg    11640

cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca    11700

cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc    11760

tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga    11820

taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac    11880

gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga    11940

agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag    12000

ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg    12060

acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag    12120

caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc    12180

tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc    12240

tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcta tggtgcactc    12300

tcagtacaat ctgctctgat gccgcatagt taagccagt                           12339


<210>  2
<211>  12306
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3XFlag-Cas9-xTen-Tn5

<400>  2
atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc       60

cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac      120

cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca      180

gctgcggtaa agctcatcag cgtggtcgtg cagcgattca cagatgtctg cctgttcatc      240

cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc      300

catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct      360

gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga      420

tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg      480

gcgggaccag agaaaaatca ctcagggtca atgccagccg aacgccagca agacgtagcc      540

cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc      600

gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag      660

gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc      720

cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt      780

catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg      840

agatcccggt gcctaatgag tgagctaact tacattaatt gcgttgcgct cactgcccgc      900

tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag      960

aggcggtttg cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca     1020

gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt     1080

gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt     1140

cttcggtatc gtcgtatccc actaccgaga tatccgcacc aacgcgcagc ccggactcgg     1200

taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa     1260

cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc     1320

cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca     1380

gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac     1440

ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac     1500

tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag     1560

cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc     1620

gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca     1680

tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt     1740

gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc     1800

ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca     1860

ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct     1920

gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca     1980

ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt     2040

cgatggtgtc cgggatctcg acgctctccc ttatgcgact cctgcattag gaagcagccc     2100

agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg ccggcatgcc     2160

gccctttcgt cttcaagaat taattcccaa ttccccaggc atcaaataaa acgaaaggct     2220

cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt     2280

aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg     2340

gcaggacgcc cgccataaac tgccaggaat taattcccca ggcatcaaat aaaacgaaag     2400

gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg     2460

agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg     2520

cgggcaggac gcccgccata aactgccagg aattaattcc ccaggcatca aataaaacga     2580

aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc     2640

ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg     2700

tggcgggcag gacgcccgcc ataaactgcc aggaattaat tccccaggca tcaaataaaa     2760

cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct     2820

ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga     2880

gggtggcggg caggacgccc gccataaact gccaggaatt aattccccag gcatcaaata     2940

aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac     3000

gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc     3060

ggagggtggc gggcaggacg cccgccataa actgccagga attggggatc ggaattaatt     3120

cccggtttaa accggggatc tcgatcccgc gaaattaata cgactcacta taggggaatt     3180

gtgagcggat aacaattccc ctctagaaat aattttgttt aactttaaga aggagatata     3240

ccatgggtga ttacaaggat cacgatggcg attacaagga tcacgatatc gattacaagg     3300

atgatgatga taagatggat aaaaagtatt ctattggttt agctatcggc acaaatagcg     3360

tcggatgggc ggtgatcact gatgaatata aggttccgtc taaaaagttc aaggttctgg     3420

gaaatacaga ccgccacagt atcaaaaaaa atcttatagg ggctctttta tttgacagtg     3480

gagagacagc ggaagcgact cgtctcaaac ggacagctcg tagaaggtat acacgtcgga     3540

agaatcgtat ttgttatcta caggagattt tttcaaatga gatggcgaaa gtagatgata     3600

gtttctttca tcgacttgaa gagtcttttt tggtggaaga agacaagaag catgaacgtc     3660

atcctatttt tggaaatata gtagatgaag ttgcttatca tgagaaatat ccaactatct     3720

atcatctgcg aaaaaaattg gtagattcta ctgataaagc ggatttgcgc ttaatctatt     3780

tggccttagc gcatatgatt aagtttcgtg gtcatttttt gattgaggga gatttaaatc     3840

ctgataatag tgatgtggac aaactattta tccagttggt acaaacctac aatcaattat     3900

ttgaagaaaa ccctattaac gcaagtggag tagatgctaa agcgattctt tctgcacgat     3960

tgagtaaatc aagacgatta gaaaatctca ttgctcagct ccccggtgag aagaaaaatg     4020

gcttatttgg gaatctcatt gctttgtcat tgggtttgac ccctaatttt aaatcaaatt     4080

ttgatttggc agaagatgct aaattacagc tttcaaaaga tacttacgat gatgatttag     4140

ataatttatt ggcgcaaatt ggagatcaat atgctgattt gtttttggca gctaagaatt     4200

tatcagatgc tattttactt tcagatatcc taagagtaaa tactgaaata actaaggctc     4260

ccctatcagc atcaatgatt aaacgctacg atgaacatca tcaagacttg actcttttaa     4320

aagctttagt tcgacaacaa cttccagaaa agtataaaga aatctttttt gatcaatcaa     4380

aaaacggata tgcaggttat attgatgggg gagctagcca agaagaattt tataaattta     4440

tcaaaccaat tttagaaaaa atggatggta ctgaggaatt attggtgaaa ctaaatcgtg     4500

aagatttgct gcgcaagcaa cggacctttg acaacggctc tattccccat caaattcact     4560

tgggtgagct gcatgctatt ttgagaagac aagaagactt ttatccattt ttaaaagaca     4620

atcgtgagaa gattgaaaaa atcttgactt ttcgaattcc ttattatgtt ggtccattgg     4680

cgcgtggcaa tagtcgtttt gcatggatga ctcggaagtc tgaagaaaca attaccccat     4740

ggaattttga agaagttgtc gataaaggtg cttcagctca atcatttatt gaacgcatga     4800

caaactttga taaaaatctt ccaaatgaaa aagtactacc aaaacatagt ttgctttatg     4860

agtattttac ggtttataac gaattgacaa aggtcaaata tgttactgaa ggaatgcgaa     4920

aaccagcatt tctttcaggt gaacagaaga aagccattgt tgatttactc ttcaaaacaa     4980

atcgaaaagt aaccgttaag caattaaaag aagattattt caaaaaaata gaatgttttg     5040

atagtgttga aatttcagga gttgaagata gatttaatgc ttcattaggt acctaccatg     5100

atttgctaaa aattattaaa gataaagatt ttttggataa tgaagaaaat gaagatatct     5160

tagaggatat tgttttaaca ttgaccttat ttgaagatag ggagatgatt gaggaaagac     5220

ttaaaacata tgctcacctc tttgatgata aggtgatgaa acagcttaaa cgtcgccgtt     5280

atactggttg gggacgtttg tctcgaaaat tgattaatgg tattagggat aagcaatctg     5340

gcaaaacaat attagatttt ttgaaatcag atggttttgc caatcgcaat tttatgcagc     5400

tgatccatga tgatagtttg acatttaaag aagacattca aaaagcacaa gtgtctggac     5460

aaggcgatag tttacatgaa catattgcaa atttagctgg tagccctgct attaaaaaag     5520

gtattttaca gactgtaaaa gttgttgatg aattggtcaa agtaatgggg cggcataagc     5580

cagaaaatat cgttattgaa atggcacgtg aaaatcagac aactcaaaag ggccagaaaa     5640

attcgcgaga gcgtatgaaa cgaatcgaag aaggtatcaa agaattagga agtcagattc     5700

ttaaagagca tcctgttgaa aatactcaat tgcaaaatga aaagctctat ctctattatc     5760

tccaaaatgg aagagacatg tatgtggacc aagaattaga tattaatcgt ttaagtgatt     5820

atgatgtcga tgccattgtt ccacaaagtt tccttaaaga cgattcaata gacaataagg     5880

tcttaacgcg ttctgataaa aatcgtggta aatcggataa cgttccaagt gaagaagtag     5940

tcaaaaagat gaaaaactat tggagacaac ttctaaacgc caagttaatc actcaacgta     6000

agtttgataa tttaacgaaa gctgaacgtg gaggtttgag tgaacttgat aaagctggtt     6060

ttatcaaacg ccaattggtt gaaactcgcc aaatcactaa gcatgtggca caaattttgg     6120

atagtcgcat gaatactaaa tacgatgaaa atgataaact tattcgagag gttaaagtga     6180

ttaccttaaa atctaaatta gtttctgact tccgaaaaga tttccaattc tataaagtac     6240

gtgagattaa caattaccat catgcccatg atgcgtatct aaatgccgtc gttggaactg     6300

ctttgattaa gaaatatcca aaacttgaat cggagtttgt ctatggtgat tataaagttt     6360

atgatgttcg taaaatgatt gctaagtctg agcaagaaat aggcaaagca accgcaaaat     6420

atttctttta ctctaatatc atgaacttct tcaaaacaga aattacactt gcaaatggag     6480

agattcgcaa acgccctcta atcgaaacta atggggaaac tggagaaatt gtctgggata     6540

aagggcgaga ttttgccaca gtgcgcaaag tattgtccat gccccaagtc aatattgtca     6600

agaaaacaga agtacagaca ggcggattct ccaaggagtc aattttacca aaaagaaatt     6660

cggacaagct tattgctcgt aaaaaagact gggatccaaa aaaatatggt ggttttgata     6720

gtccaacggt agcttattca gtcctagtgg ttgctaaggt ggaaaaaggg aaatcgaaga     6780

agttaaaatc cgttaaagag ttactaggga tcacaattat ggaaagaagt tcctttgaaa     6840

aaaatccgat tgacttttta gaagctaaag gatataagga agttaaaaaa gacttaatca     6900

ttaaactacc taaatatagt ctttttgagt tagaaaacgg tcgtaaacgg atgctggcta     6960

gtgccggaga attacaaaaa ggaaatgagc tggctctgcc aagcaaatat gtgaattttt     7020

tatatttagc tagtcattat gaaaagttga agggtagtcc agaagataac gaacaaaaac     7080

aattgtttgt ggagcagcat aagcattatt tagatgagat tattgagcaa atcagtgaat     7140

tttctaagcg tgttatttta gcagatgcca atttagataa agttcttagt gcatataaca     7200

aacatagaga caaaccaata cgtgaacaag cagaaaatat tattcattta tttacgttga     7260

cgaatcttgg agctcccgct gcttttaaat attttgatac aacaattgat cgtaaacgat     7320

atacgtctac aaaagaagtt ttagatgcca ctcttatcca tcaatccatc actggtcttt     7380

atgaaacacg cattgatttg agtcagctag gaggtgacag cggttccgaa actcccggta     7440

catcagaaag cgcgaccccc gaaagcatga ttaccagtgc actgcatcgt gcggcggatt     7500

gggcgaaaag cgtgttttct agtgctgcgc tgggtgatcc gcgtcgtacc gcgcgtctgg     7560

tgaatgttgc ggcgcaactg gccaaatata gcggcaaaag cattaccatt agcagcgaag     7620

gcagcaaagc catgcaggaa ggcgcgtatc gttttattcg taatccgaac gtgagcgcgg     7680

aagcgattcg taaagcgggt gccatgcaga ccgtgaaact ggcccaggaa tttccggaac     7740

tgctggcaat tgaagatacc acctctctga gctatcgtca tcaggtggcg gaagaactgg     7800

gcaaactggg tagcattcag gataaaagcc gtggttggtg ggtgcatagc gtgctgctgc     7860

tggaagcgac cacctttcgt accgtgggcc tgctgcatca agaatggtgg atgcgtccgg     7920

atgatccggc ggatgcggat gaaaaagaaa gcggcaaatg gctggccgct gctgcaactt     7980

cgcgtctgag aatgggcagc atgatgagca acgtgattgc ggtgtgcgat cgtgaagcgg     8040

atattcatgc gtatctgcaa gataaactgg cccataacga acgttttgtg gtgcgtagca     8100

aacatccgcg taaagatgtg gaaagcggcc tgtatctgta tgatcacctg aaaaaccagc     8160

cggaactggg cggctatcag attagcattc cgcagaaagg cgtggtggat aaacgtggca     8220

aacgtaaaaa ccgtccggcg cgtaaagcga gcctgagcct gcgtagcggc cgtattaccc     8280

tgaaacaggg caacattacc ctgaacgcgg tgctggccga agaaattaat ccgccgaaag     8340

gcgaaacccc gctgaaatgg ctgctgctga ccagcgagcc ggtggaaagt ctggcccaag     8400

cgctgcgtgt gattgatatt tatacccatc gttggcgcat tgaagaattt cacaaagcgt     8460

ggaaaacggg tgcgggtgcg gaacgtcagc gtatggaaga accggataac ctggaacgta     8520

tggtgagcat tctgagcttt gtggcggtgc gtctgctgca actgcgtgaa tcttttactc     8580

cgccgcaagc actgcgtgcg cagggcctgc tgaaagaagc ggaacacgtt gaaagccaga     8640

gcgcggaaac cgtgctgacc ccggatgaat gccaactgct gggctatctg gataaaggca     8700

aacgcaaacg caaagaaaaa gcgggcagcc tgcaatgggc gtatatggcg attgcgcgtc     8760

tgggcggctt tatggatagc aaacgtaccg gcattgcgag ctggggtgcg ctgtgggaag     8820

gttgggaagc gctgcaaagc aaactggatg gctttctggc cgcgaaagac ctgatggcgc     8880

agggcattaa aatctgcatc acgggagatg cactagttgc cctacccgag ggcgagtcgg     8940

tacgcatcgc cgacatcgtg ccgggtgcgc ggcccaacag tgacaacgcc atcgacctga     9000

aagtccttga ccggcatggc aatcccgtgc tcgccgaccg gctgttccac tccggcgagc     9060

atccggtgta cacggtgcgt acggtcgaag gtctgcgtgt gacgggcacc gcgaaccacc     9120

cgttgttgtg tttggtcgac gtcgccgggg tgccgaccct gctgtggaag ctgatcgacg     9180

aaatcaagcc gggcgattac gcggtgattc aacgcagcgc attcagcgtc gactgtgcag     9240

gttttgcccg cgggaaaccc gaatttgcgc ccacaaccta cacagtcggc gtccctggac     9300

tggtgcgttt cttggaagca caccaccgag acccggacgc ccaagctatc gccgacgagc     9360

tgaccgacgg gcggttctac tacgcgaaag tcgccagtgt caccgacgcc ggcgtgcagc     9420

cggtgtatag ccttcgtgtc gacacggcag accacgcgtt tatcacgaac gggttcgtca     9480

gccacgctac tggcctcacc ggtctgaact caggcctcac gacaaatcct ggtgtatccg     9540

cttggcaggt caacacagct tatactgcgg gacaattggt cacatataac ggcaagacgt     9600

ataaatgttt gcagccccac acctccttgg caggatggga accatccaac gttcctgcct     9660

tgtggcagct tcaatgactg caggaagggg atccggctgc taacaaagcc cgaaaggaag     9720

ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac     9780

gggtcttgag gggttttttg ctgaaaggag gaactatatc cggataacta cgtcaggtgg     9840

cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa     9900

tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa     9960

gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct    10020

tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg    10080

tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg    10140

ccccgaagaa cgtttcccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt    10200

atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga    10260

cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga    10320

attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac    10380

gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg    10440

ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac    10500

gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct    10560

agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct    10620

gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg    10680

gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat    10740

ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg    10800

tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat    10860

tgatttaccc cggttgataa tcagaaaagc cccaaaaaca ggaagattgt ataagcaaat    10920

atttaaattg taaacgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc    10980

tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagccc    11040

gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac    11100

tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca    11160

cccaaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg    11220

agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag    11280

aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc    11340

accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt aaaaggatct aggtgaagat    11400

cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc    11460

agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg    11520

ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct    11580

accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct    11640

tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct    11700

cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg    11760

gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc    11820

gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga    11880

gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg    11940

cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta    12000

tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg    12060

ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg    12120

ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat    12180

taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc    12240

agtgagcgag gaagctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa    12300

gccagt                                                               12306


<210>  3
<211>  11245
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pET-Tn5-xTen-dCas9

<400>  3
caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa       60

acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat      120

ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta      180

gaggatcgag atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg      240

gataacaatt cccctctaga aataattttg tttaacttta agaaggagat ataccatgat      300

taccagtgca ctgcatcgtg cggcggattg ggcgaaaagc gtgttttcta gtgctgcgct      360

gggtgatccg cgtcgtaccg cgcgtctggt gaatgttgcg gcgcaactgg ccaaatatag      420

cggcaaaagc attaccatta gcagcgaagg cagcaaagcc atgcaggaag gcgcgtatcg      480

ttttattcgt aatccgaacg tgagcgcgga agcgattcgt aaagcgggtg ccatgcagac      540

cgtgaaactg gcccaggaat ttccggaact gctggcaatt gaagatacca cctctctgag      600

ctatcgtcat caggtggcgg aagaactggg caaactgggt agcattcagg ataaaagccg      660

tggttggtgg gtgcatagcg tgctgctgct ggaagcgacc acctttcgta ccgtgggcct      720

gctgcatcaa gaatggtgga tgcgtccgga tgatccggcg gatgcggatg aaaaagaaag      780

cggcaaatgg ctggccgctg ctgcaacttc gcgtctgaga atgggcagca tgatgagcaa      840

cgtgattgcg gtgtgcgatc gtgaagcgga tattcatgcg tatctgcaag ataaactggc      900

ccataacgaa cgttttgtgg tgcgtagcaa acatccgcgt aaagatgtgg aaagcggcct      960

gtatctgtat gatcacctga aaaaccagcc ggaactgggc ggctatcaga ttagcattcc     1020

gcagaaaggc gtggtggata aacgtggcaa acgtaaaaac cgtccggcgc gtaaagcgag     1080

cctgagcctg cgtagcggcc gtattaccct gaaacagggc aacattaccc tgaacgcggt     1140

gctggccgaa gaaattaatc cgccgaaagg cgaaaccccg ctgaaatggc tgctgctgac     1200

cagcgagccg gtggaaagtc tggcccaagc gctgcgtgtg attgatattt atacccatcg     1260

ttggcgcatt gaagaatttc acaaagcgtg gaaaacgggt gcgggtgcgg aacgtcagcg     1320

tatggaagaa ccggataacc tggaacgtat ggtgagcatt ctgagctttg tggcggtgcg     1380

tctgctgcaa ctgcgtgaat cttttactcc gccgcaagca ctgcgtgcgc agggcctgct     1440

gaaagaagcg gaacacgttg aaagccagag cgcggaaacc gtgctgaccc cggatgaatg     1500

ccaactgctg ggctatctgg ataaaggcaa acgcaaacgc aaagaaaaag cgggcagcct     1560

gcaatgggcg tatatggcga ttgcgcgtct gggcggcttt atggatagca aacgtaccgg     1620

cattgcgagc tggggtgcgc tgtgggaagg ttgggaagcg ctgcaaagca aactggatgg     1680

ctttctggcc gcgaaagacc tgatggcgca gggcattaaa atcagcggtt ccgaaactcc     1740

cggtacatca gaaagcgcga cccccgaaag catggataaa aagtattcta ttggtttagc     1800

tatcggcaca aatagcgtcg gatgggcggt gatcactgat gaatataagg ttccgtctaa     1860

aaagttcaag gttctgggaa atacagaccg ccacagtatc aaaaaaaatc ttataggggc     1920

tcttttattt gacagtggag agacagcgga agcgactcgt ctcaaacgga cagctcgtag     1980

aaggtataca cgtcggaaga atcgtatttg ttatctacag gagatttttt caaatgagat     2040

ggcgaaagta gatgatagtt tctttcatcg acttgaagag tcttttttgg tggaagaaga     2100

caagaagcat gaacgtcatc ctatttttgg aaatatagta gatgaagttg cttatcatga     2160

gaaatatcca actatctatc atctgcgaaa aaaattggta gattctactg ataaagcgga     2220

tttgcgctta atctatttgg ccttagcgca tatgattaag tttcgtggtc attttttgat     2280

tgagggagat ttaaatcctg ataatagtga tgtggacaaa ctatttatcc agttggtaca     2340

aacctacaat caattatttg aagaaaaccc tattaacgca agtggagtag atgctaaagc     2400

gattctttct gcacgattga gtaaatcaag acgattagaa aatctcattg ctcagctccc     2460

cggtgagaag aaaaatggct tatttgggaa tctcattgct ttgtcattgg gtttgacccc     2520

taattttaaa tcaaattttg atttggcaga agatgctaaa ttacagcttt caaaagatac     2580

ttacgatgat gatttagata atttattggc gcaaattgga gatcaatatg ctgatttgtt     2640

tttggcagct aagaatttat cagatgctat tttactttca gatatcctaa gagtaaatac     2700

tgaaataact aaggctcccc tatcagcatc aatgattaaa cgctacgatg aacatcatca     2760

agacttgact cttttaaaag ctttagttcg acaacaactt ccagaaaagt ataaagaaat     2820

cttttttgat caatcaaaaa acggatatgc aggttatatt gatgggggag ctagccaaga     2880

agaattttat aaatttatca aaccaatttt agaaaaaatg gatggtactg aggaattatt     2940

ggtgaaacta aatcgtgaag atttgctgcg caagcaacgg acctttgaca acggctctat     3000

tccccatcaa attcacttgg gtgagctgca tgctattttg agaagacaag aagactttta     3060

tccattttta aaagacaatc gtgagaagat tgaaaaaatc ttgacttttc gaattcctta     3120

ttatgttggt ccattggcgc gtggcaatag tcgttttgca tggatgactc ggaagtctga     3180

agaaacaatt accccatgga attttgaaga agttgtcgat aaaggtgctt cagctcaatc     3240

atttattgaa cgcatgacaa actttgataa aaatcttcca aatgaaaaag tactaccaaa     3300

acatagtttg ctttatgagt attttacggt ttataacgaa ttgacaaagg tcaaatatgt     3360

tactgaagga atgcgaaaac cagcatttct ttcaggtgaa cagaagaaag ccattgttga     3420

tttactcttc aaaacaaatc gaaaagtaac cgttaagcaa ttaaaagaag attatttcaa     3480

aaaaatagaa tgttttgata gtgttgaaat ttcaggagtt gaagatagat ttaatgcttc     3540

attaggtacc taccatgatt tgctaaaaat tattaaagat aaagattttt tggataatga     3600

agaaaatgaa gatatcttag aggatattgt tttaacattg accttatttg aagataggga     3660

gatgattgag gaaagactta aaacatatgc tcacctcttt gatgataagg tgatgaaaca     3720

gcttaaacgt cgccgttata ctggttgggg acgtttgtct cgaaaattga ttaatggtat     3780

tagggataag caatctggca aaacaatatt agattttttg aaatcagatg gttttgccaa     3840

tcgcaatttt atgcagctga tccatgatga tagtttgaca tttaaagaag acattcaaaa     3900

agcacaagtg tctggacaag gcgatagttt acatgaacat attgcaaatt tagctggtag     3960

ccctgctatt aaaaaaggta ttttacagac tgtaaaagtt gttgatgaat tggtcaaagt     4020

aatggggcgg cataagccag aaaatatcgt tattgaaatg gcacgtgaaa atcagacaac     4080

tcaaaagggc cagaaaaatt cgcgagagcg tatgaaacga atcgaagaag gtatcaaaga     4140

attaggaagt cagattctta aagagcatcc tgttgaaaat actcaattgc aaaatgaaaa     4200

gctctatctc tattatctcc aaaatggaag agacatgtat gtggaccaag aattagatat     4260

taatcgttta agtgattatg atgtcgatgc cattgttcca caaagtttcc ttaaagacga     4320

ttcaatagac aataaggtct taacgcgttc tgataaaaat cgtggtaaat cggataacgt     4380

tccaagtgaa gaagtagtca aaaagatgaa aaactattgg agacaacttc taaacgccaa     4440

gttaatcact caacgtaagt ttgataattt aacgaaagct gaacgtggag gtttgagtga     4500

acttgataaa gctggtttta tcaaacgcca attggttgaa actcgccaaa tcactaagca     4560

tgtggcacaa attttggata gtcgcatgaa tactaaatac gatgaaaatg ataaacttat     4620

tcgagaggtt aaagtgatta ccttaaaatc taaattagtt tctgacttcc gaaaagattt     4680

ccaattctat aaagtacgtg agattaacaa ttaccatcat gcccatgatg cgtatctaaa     4740

tgccgtcgtt ggaactgctt tgattaagaa atatccaaaa cttgaatcgg agtttgtcta     4800

tggtgattat aaagtttatg atgttcgtaa aatgattgct aagtctgagc aagaaatagg     4860

caaagcaacc gcaaaatatt tcttttactc taatatcatg aacttcttca aaacagaaat     4920

tacacttgca aatggagaga ttcgcaaacg ccctctaatc gaaactaatg gggaaactgg     4980

agaaattgtc tgggataaag ggcgagattt tgccacagtg cgcaaagtat tgtccatgcc     5040

ccaagtcaat attgtcaaga aaacagaagt acagacaggc ggattctcca aggagtcaat     5100

tttaccaaaa agaaattcgg acaagcttat tgctcgtaaa aaagactggg atccaaaaaa     5160

atatggtggt tttgatagtc caacggtagc ttattcagtc ctagtggttg ctaaggtgga     5220

aaaagggaaa tcgaagaagt taaaatccgt taaagagtta ctagggatca caattatgga     5280

aagaagttcc tttgaaaaaa atccgattga ctttttagaa gctaaaggat ataaggaagt     5340

taaaaaagac ttaatcatta aactacctaa atatagtctt tttgagttag aaaacggtcg     5400

taaacggatg ctggctagtg ccggagaatt acaaaaagga aatgagctgg ctctgccaag     5460

caaatatgtg aattttttat atttagctag tcattatgaa aagttgaagg gtagtccaga     5520

agataacgaa caaaaacaat tgtttgtgga gcagcataag cattatttag atgagattat     5580

tgagcaaatc agtgaatttt ctaagcgtgt tattttagca gatgccaatt tagataaagt     5640

tcttagtgca tataacaaac atagagacaa accaatacgt gaacaagcag aaaatattat     5700

tcatttattt acgttgacga atcttggagc tcccgctgct tttaaatatt ttgatacaac     5760

aattgatcgt aaacgatata cgtctacaaa agaagtttta gatgccactc ttatccatca     5820

atccatcact ggtctttatg aaacacgcat tgatttgagt cagctaggag gtgaccacca     5880

ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc     5940

tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg     6000

ttttttgctg aaaggaggaa ctatatccgg atatcccgca agaggcccgg cagtaccggc     6060

ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca     6120

ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg     6180

cattaaagct agcttatcga tgataagctg tcaaacatga gaattaattc ttgaagacga     6240

aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag     6300

acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa     6360

atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat     6420

tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg     6480

gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa     6540

gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt     6600

gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt     6660

ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat     6720

tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg     6780

acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta     6840

cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat     6900

catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag     6960

cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa     7020

ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca     7080

ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc     7140

ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt     7200

atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc     7260

gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat     7320

atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt     7380

tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac     7440

cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc     7500

ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca     7560

actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta     7620

gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct     7680

ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg     7740

gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc     7800

acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta     7860

tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg     7920

gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt     7980

cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg     8040

cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg     8100

ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc     8160

gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg     8220

agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt     8280

tcacaccgca atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt     8340

atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc caccaacacc     8400

cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac     8460

cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca     8520

gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc     8580

cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc     8640

catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct     8700

gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga     8760

tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg     8820

gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg     8880

tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg     8940

cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt     9000

tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga     9060

ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag     9120

cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct     9180

gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt     9240

tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg     9300

ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg     9360

cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag     9420

gcggcataaa tcgccgtgac gatcagcggt ccaatgatcg aagttaggct ggtaagagcc     9480

gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc     9540

tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc     9600

cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg     9660

ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg     9720

agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga     9780

aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg     9840

ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag     9900

ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc     9960

taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc    10020

cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag    10080

ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc    10140

ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt    10200

gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac    10260

cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc    10320

catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat    10380

ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat    10440

ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact    10500

taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc    10560

cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac    10620

atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc    10680

atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc    10740

cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag    10800

ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact    10860

ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt    10920

gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac    10980

gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc    11040

gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg    11100

ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct    11160

ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca    11220

ccgccgccgc aaggaatggt gcatg                                          11245


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD.Cas9.CVSJ0588.AF

<400>  4
gaaattaatg gtttaagctt                                                   20


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD.Cas9.CVSJ0588.AA

<400>  5
aggtgagcaa gatttccatt                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD.Cas9.CVSJ0588.AC

<400>  6
tcaaggacat attctcctgt                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD.Cas9.CVSJ0588.AJ

<400>  7
aatgtgctcc ataaggaatt                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD.Cas9.CVSJ0588.AG

<400>  8
aactttcttc ttctgaggag                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD.Cas9.CVSJ0588.AB

<400>  9
aagatcacac ctatgggaaa                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD.Cas9.RNXS0617.AA

<400>  10
gctattttga ccatttcaat                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD.Cas9.RNXS0617.AB

<400>  11
cggaggacaa atccatacca                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD.Cas9.RNXS0617.AC

<400>  12
cagtttatcg ttattaccaa                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD.Cas9.RNXS0617.AD

<400>  13
acttatacca tgctgaccat                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD.Cas9.RZZJ8230.AA

<400>  14
ggacaacacc ctgaccatcc                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD.Cas9.RZZJ8230.AC

<400>  15
gtctgacctc gactccatcc                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD.Cas9.RZZJ8230.AD

<400>  16
gaacatcaaa ggtctgactc                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EXT1-1

<400>  17
atatcacgtc cataacgggg                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EXT1-4

<400>  18
cacttggcct gactacaccg                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BCL9-2

<400>  19
gggttggcat cggaaccacg                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BCL9-4

<400>  20
gatgccctct ccaaatgccg                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HOXA13-1

<400>  21
gtagccatag ggcagcgccg                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HOXA13-4

<400>  22
tttctctacg acaacggcgg                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HOXD11-1

<400>  23
gggcttcgac cagttctacg                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HOXD11-3

<400>  24
gggctacgct ccctactacg                                                   20


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OLIG2-1

<400>  25
actggtgagc gagatctacg                                                   20


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OLIG2-2

<400>  26
gcacgccgca catcaccccg                                                   20


<210>  27
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Adaptor A

<400>  27
tcgtcggcag cgtcagatgt gtataagaga cag                                    33


<210>  28
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Adaptor B

<400>  28
gtctcgtggg ctcggagatg tgtataagag acag                                   34


<210>  29
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mosaic end

<400>  29
agatgtgtat aagagacag                                                    19


<210>  30
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Illumina Sequence Primer Site-SP2

<400>  30
gtctcgtggg ctcgg                                                        15


<210>  31
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Illumina Sequence Primer Site-SP1

<400>  31
tcgtcggcag cgtc                                                         14


<210>  32
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (G)n


<220>
<221>  Variant
<222>  (2)..(10)
<223>  May be present or absent

<400>  32

Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly 
1               5                   10  


<210>  33
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (GS)n


<220>
<221>  Variant
<222>  (3)..(20)
<223>  May be present or absent

<400>  33

Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser 
1               5                   10                  15      


Gly Ser Gly Ser 
            20  


<210>  34
<211>  50
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (GSGGS)n


<220>
<221>  Variant
<222>  (6)..(50)
<223>  May be present or absent

<400>  34

Gly Ser Gly Gly Ser Gly Ser Gly Gly Ser Gly Ser Gly Gly Ser Gly 
1               5                   10                  15      


Ser Gly Gly Ser Gly Ser Gly Gly Ser Gly Ser Gly Gly Ser Gly Ser 
            20                  25                  30          


Gly Gly Ser Gly Ser Gly Gly Ser Gly Ser Gly Gly Ser Gly Ser Gly 
        35                  40                  45              


Gly Ser 
    50  


<210>  35
<211>  50
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (G4S)n


<220>
<221>  Variant
<222>  (6)..(50)
<223>  May be present or absent

<400>  35

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
            20                  25                  30          


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        35                  40                  45              


Gly Ser 
    50  


<210>  36
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (GGGS)n


<220>
<221>  Variant
<222>  (5)..(40)
<223>  May be present or absent

<400>  36

Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
            20                  25                  30          


Gly Gly Gly Ser Gly Gly Gly Ser 
        35                  40  


<210>  37
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  glycine/serine spacer 1

<400>  37

Gly Gly Ser Gly 
1               


<210>  38
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  glycine/serine spacer 2

<400>  38

Gly Gly Ser Gly Gly 
1               5   


<210>  39
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  glycine/serine spacer 3

<400>  39

Gly Ser Gly Ser Gly 
1               5   


<210>  40
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  glycine/serine spacer 4

<400>  40

Gly Ser Gly Gly Gly 
1               5   


<210>  41
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  glycine/serine spacer 5

<400>  41

Gly Gly Gly Ser Gly 
1               5   


<210>  42
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  glycine/serine spacer 6

<400>  42

Gly Ser Ser Ser Gly 
1               5   


<210>  43
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  XTEN linker 1

<400>  43

Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala 
1               5                   10          


<210>  44
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  XTEN linker 2

<400>  44

Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser 
1               5                   10                  15      


<210>  45
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  XTEN linker 3

<400>  45

Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Gly 
1               5                   10                  15      


Gly Ser Gly Gly Ser 
            20      


