                         SEQUENCE LISTING

<110>  THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
 
<120>  Tumor Immunotherapy

<130>  0019240.01231WO1

<140>  PCT/US2022/028915
<141>  2022-05-12

<150>  US63/188970
<151>  2021-05-14

<150>  US63/313251
<151>  2022-02-23

<160>  124   

<170>  PatentIn version 3.5

<210>  1
<211>  200
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRISPRko oligo library


<220>
<221>  misc_feature
<222>  (91)..(110)
<223>  n is a, c, g, or t

<400>  1
acacgtcata tagatgccgt cctagcgagc gtggagtgag ccattgtgag cgctcacaat       60

tatatatctt gtggaaagga cgaaacaccg nnnnnnnnnn nnnnnnnnnn gttttagagc      120

tagaaatagc aagttaaaat aaggctagtc cgttatcatc ggcagcaacc agatgggcac      180

aggaaagata cttaacgctt                                                  200


<210>  2
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TREG_1F Primer

<400>  2
agcgtggagt gagcc                                                        15


<210>  3
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TREG_1R Primer

<400>  3
tctggttgct gccga                                                        15


<210>  4
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TREG_2F Primer

<400>  4
agcgctcaca attatatatc ttgtggaaag gacgaaacac cg                          42


<210>  5
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TREG_2R Primer

<400>  5
cggactagcc ttattttaac ttgctatttc tagctctaaa ac                          42


<210>  6
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TREG_NGS_1F Primer

<400>  6
ggactatcat atgcttaccg taacttgaaa gtaattgt                               38


<210>  7
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TREG_NGS_1R Primer

<400>  7
gaagatccgg gtgacgctgc gaacggacgt                                        30


<210>  8
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TREG_NGS_2F Primer

<400>  8
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tccgatcttt       60

gtggaaagga cgaaacaccg                                                   80


<210>  9
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TREG_NGS_2R Primer


<220>
<221>  misc_feature
<222>  (25)..(32)
<223>  n is a, c, g, or t

<400>  9
caagcagaag acggcatacg agatnnnnnn nngtgactgg agttcagacg tgtgctcttc       60

cgatcttcta ctattctttc ccctgcactg t                                      91


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRPS1_1 guide sequence

<400>  10
agaggggcag acatcctacg                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRPS1_2 guide sequence

<400>  11
agcatcggat gtcaaacagg                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Non-targeting guide 1 sequence

<400>  12
gcgaggtatt cggctccgcg                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Non-targeting guide 2 sequence

<400>  13
gctttcacgg aggttcgacg                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Egr1 sgRNA Sequence

<400>  14
gaggattggt catgctcacg                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Egr1 sgRNA Sequence

<400>  15
gctcatccga gcgagaaaag                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Egr1 sgRNA Sequence

<400>  16
gttatcccag ccaaacgact                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nr3c1 sgRNA Sequence

<400>  17
cattatgggg tgctgacgtg                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nr3c1 sgRNA Sequence

<400>  18
agaccgaaac aaaagtgatg                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nr3c1 sgRNA Sequence

<400>  19
ctcttgctta attaccccag                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pbx4 sgRNA Sequence

<400>  20
ctgtctgtag gcgcaagcgg                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pbx4 sgRNA Sequence

<400>  21
tcgctcgtgt catggcctag                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pbx4 sgRNA sequence

<400>  22
atgtgtggtg aactcacgac                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mafb sgRNA Sequence

<400>  23
tgaaccgaag acccatctcg                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mafb sgRNA Sequence

<400>  24
ggtgtgactc acgatgacct                                                   20


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mafb sgRNA Sequence

<400>  25
agctacccac tagccacccg                                                   20


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Id2 sgRNA Sequence

<400>  26
ctcccggagc aaaaccccgg                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Id2 sgRNA Sequence

<400>  27
gtgatgcagg ctgacgatag                                                   20


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Id2 sgRNA Sequence

<400>  28
gcacgtcatc gattacatct                                                   20


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Stat 4 sgRNA Sequence

<400>  29
aatgtctaaa ctccactgag                                                   20


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Stat 4 sgRNA Sequence

<400>  30
gcatgccaac gcaccctcag                                                   20


<210>  31
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Stat 4 sgRNA Sequence

<400>  31
cagccaacta cctaatgcat                                                   20


<210>  32
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nr4a3 sgRNA Sequence

<400>  32
gcattcatca tacagatcgg                                                   20


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nr4a3 sgRNA Sequence

<400>  33
accaagctga ccatggacct                                                   20


<210>  34
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nr4a3 sgRNA Sequence

<400>  34
agtgtcggga tggttaagga                                                   20


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nr4a1 sgRNA Sequence

<400>  35
ggaagtgtca cggttcggag                                                   20


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nr4a1 sgRNA Sequence

<400>  36
ggcaaacaag gattgccctg                                                   20


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nr4a1 sgRNA Sequence

<400>  37
gccttcataa gtctggctcg                                                   20


<210>  38
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trps1 sgRNA Sequence

<400>  38
agaggggcag acatcctacg                                                   20


<210>  39
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trps1 sgRNA Sequence

<400>  39
agcatcggat gtcaaacagg                                                   20


<210>  40
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trps1 sgRNA Sequence

<400>  40
taggactgca taatcgcacc                                                   20


<210>  41
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Egr3 sgRNA Sequence

<400>  41
taccaccatc ccaacgacat                                                   20


<210>  42
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Egr3 sgRNA Sequence

<400>  42
ggccgattgg taatcctggg                                                   20


<210>  43
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Egr3 sgRNA Sequence

<400>  43
gctcagtacg cagacgtcca                                                   20


<210>  44
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Banp sgRNA Sequence

<400>  44
tgatgtgcaa catgtcggaa                                                   20


<210>  45
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Banp sgRNA Sequence

<400>  45
agacctgcaa caaagtgcga                                                   20


<210>  46
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Banp sgRNA Sequence

<400>  46
gcgtcggcag aacaccatcg                                                   20


<210>  47
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Zeb2 sgRNA Sequence

<400>  47
tatgaatagt aacttgagtg                                                   20


<210>  48
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Zeb2 sgRNA Sequence

<400>  48
gtaccttcag cgaagcgaca                                                   20


<210>  49
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Zeb2 sgRNA Sequence

<400>  49
gcgaacgtgt agctacaaag                                                   20


<210>  50
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Klf4 sgRNA Sequence

<400>  50
cgcgacactc acgttagtcg                                                   20


<210>  51
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Klf4 sgRNA Sequence

<400>  51
gctaacccac caggaatcgg                                                   20


<210>  52
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Klf4 sgRNA Sequence

<400>  52
agggcgccac taccacgggg                                                   20


<210>  53
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gli1 sgRNA Sequence

<400>  53
atgttgccat ggatactagg                                                   20


<210>  54
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gli1 sgRNA Sequence

<400>  54
gtaccacatg actctactcg                                                   20


<210>  55
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gli1 sgRNA Sequence

<400>  55
agaagccgaa ccgagtaccc                                                   20


<210>  56
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Csrnp2 sgRNA Sequence

<400>  56
agtttaaccc gatccgagtg                                                   20


<210>  57
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Csrnp2 sgRNA Sequence

<400>  57
atcatcgagt gtcaagccgt                                                   20


<210>  58
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Csrnp2 sgRNA Sequence

<400>  58
aaggccgagg aggactcgag                                                   20


<210>  59
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kdm2b sgRNA Sequence

<400>  59
gggggacgtt tgaagcgacg                                                   20


<210>  60
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kdm2b sgRNA Sequence

<400>  60
gccacacaca aggcacacgg                                                   20


<210>  61
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kdm2b sgRNA Sequence

<400>  61
ccactcaggt tggatccatg                                                   20


<210>  62
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fosl2 sgRNA Sequence

<400>  62
ggagaagcgt cgaatccgga                                                   20


<210>  63
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fosl2 sgRNA Sequence

<400>  63
tggataggga ttggacatgg                                                   20


<210>  64
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fosl2 sgRNA Sequence

<400>  64
caagaccatc ggtaccaccg                                                   20


<210>  65
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Foxp3 sgRNA Sequence

<400>  65
tctacccaca gggatcaatg                                                   20


<210>  66
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Foxp3 sgRNA Sequence

<400>  66
catacctgat gcatgaagtg                                                   20


<210>  67
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Foxp3 sgRNA Sequence

<400>  67
aggtcgggac ctgcgaagtg                                                   20


<210>  68
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cd4 sgRNA Sequence

<400>  68
actcaccctc aagatacccc                                                   20


<210>  69
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cd4 sgRNA Sequence

<400>  69
tcaaaacgat caaactgcga                                                   20


<210>  70
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cd4 sgRNA Sequence

<400>  70
tatcacggcc tataagagtg                                                   20


<210>  71
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Rb1cc1 sgRNA Sequence

<400>  71
acaagtacac gtggaaacga                                                   20


<210>  72
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Rb1cc1 sgRNA Sequence

<400>  72
ctaacagctc tattacaagg                                                   20


<210>  73
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Rb1cc1 sgRNA Sequence

<400>  73
tcaagataga cccaatgatg                                                   20


<210>  74
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E2f7 sgRNA Sequence

<400>  74
ggtataggtt cggagaacgc                                                   20


<210>  75
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E2f7 sgRNA Sequence

<400>  75
tctgcgcaga atggtccaag                                                   20


<210>  76
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E2f7 sgRNA Sequence

<400>  76
gaattgaaga aggaagcata                                                   20


<210>  77
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nhlh1 sgRNA Sequence

<400>  77
acagtcgcta aagcccgact                                                   20


<210>  78
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nhlh1 sgRNA Sequence

<400>  78
cccgcgtggc gtgtgccgtg                                                   20


<210>  79
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nhlh1 sgRNA Sequence

<400>  79
ccccggatcc agcaccatcg                                                   20


<210>  80
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nr6a1 sgRNA Sequence

<400>  80
catagtgcaa gcccgtagcg                                                   20


<210>  81
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nr6a1 sgRNA Sequence

<400>  81
cacccatctt caatcaacat                                                   20


<210>  82
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nr6a1 sgRNA Sequence

<400>  82
ctgcctaaag agcaactcgt                                                   20


<210>  83
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Scx sgRNA Sequence

<400>  83
gggtacaggt agcgacccgg                                                   20


<210>  84
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Scx sgRNA Sequence

<400>  84
gggtacaggt agcgacccgg                                                   20


<210>  85
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Scx sgRNA Sequence

<400>  85
caggtgagaa atgtagctgg                                                   20


<210>  86
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Scx sgRNA Sequence

<400>  86
gggtcctggc ccgctacccg                                                   20


<210>  87
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Zbtb4 sgRNA Sequence

<400>  87
acgaggctca gtgtcgacga                                                   20


<210>  88
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Zbtb4 sgRNA Sequence

<400>  88
agtgagaaga cacccaatgg                                                   20


<210>  89
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Zbtb4 sgRNA Sequence

<400>  89
ggcacagcaa tgtacactcg                                                   20


<210>  90
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Zfp609 sgRNA Sequence

<400>  90
tggtgtcaac acatgcgacg                                                   20


<210>  91
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Zfp609 sgRNA Sequence

<400>  91
tcggggtagt actatctagg                                                   20


<210>  92
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Zfp609 sgRNA Sequence

<400>  92
gaaaggctct cccataacag                                                   20


<210>  93
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Znfx1 sgRNA Sequence

<400>  93
gggttcccta atcgagattg                                                   20


<210>  94
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Znfx1 sgRNA Sequence

<400>  94
ggtactggtt ccgaatgtcg                                                   20


<210>  95
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Znfx1 sgRNA Sequence

<400>  95
tgaacgccag taccgcacat                                                   20


<210>  96
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ahctf1 sgRNA Sequence

<400>  96
cgacacgaac cgtcaaacag                                                   20


<210>  97
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ahctf1 sgRNA Sequence

<400>  97
catgagagga gtttaaaccg                                                   20


<210>  98
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ahctf1 sgRNA Sequence

<400>  98
gtgatcgtga ccctcgattg                                                   20


<210>  99
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Brwd1 sgRNA Sequence

<400>  99
ttcctagtgg agatacaccg                                                   20


<210>  100
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Brwd1 sgRNA Sequence

<400>  100
ctgattggac agctgacacg                                                   20


<210>  101
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Brwd1 sgRNA Sequence

<400>  101
gctgtattga gagtgactgg                                                   20


<210>  102
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chd9 sgRNA Sequence

<400>  102
agaaaccatt agtttccgcg                                                   20


<210>  103
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chd9 sgRNA Sequence

<400>  103
caattgaatc agaaggacgt                                                   20


<210>  104
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chd9 sgRNA Sequence

<400>  104
tattgcctca ttcactatcg                                                   20


<210>  105
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Srfbp1 sgRNA Sequence

<400>  105
agcctcaacc aaaaccccgg                                                   20


<210>  106
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Srfbp1 sgRNA Sequence

<400>  106
ttctaccgcg accgacagag                                                   20


<210>  107
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Srfbp1 sgRNA Sequence

<400>  107
atgcactgtt gaaaaaccaa                                                   20


<210>  108
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Prdm8 sgRNA Sequence

<400>  108
gaagtatggc tcagtacaca                                                   20


<210>  109
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Prdm8 sgRNA Sequence

<400>  109
gagctcacaa aatgaacggt                                                   20


<210>  110
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Prdm8 sgRNA Sequence

<400>  110
tcgcctccgg ggatgacgcg                                                   20


<210>  111
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cdk1 sgRNA Sequence

<400>  111
gatacgagtg tacacacacg                                                   20


<210>  112
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cdk1 sgRNA Sequence

<400>  112
ctcggctcgt tactccactc                                                   20


<210>  113
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cdk1 sgRNA Sequence

<400>  113
gaagatcaga cttgaaagcg                                                   20


<210>  114
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plk1 sgRNA Sequence

<400>  114
gagcgattga aaaccttggt                                                   20


<210>  115
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plk1 sgRNA Sequence

<400>  115
gccggcggca gtatgtacgg                                                   20


<210>  116
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plk1 sgRNA Sequence

<400>  116
ccactagcaa ggtatacctg                                                   20


<210>  117
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TREG_NGS_2F Primer


<220>
<221>  misc_feature
<222>  (59)..(59)
<223>  n is a, c, g, or t

<400>  117
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tccgatctnt       60

tgtggaaagg acgaaacacc g                                                 81


<210>  118
<211>  82
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TREG_NGS_2F Primer


<220>
<221>  misc_feature
<222>  (59)..(60)
<223>  n is a, c, g, or t

<400>  118
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tccgatctnn       60

ttgtggaaag gacgaaacac cg                                                82


<210>  119
<211>  83
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TREG_NGS_2F Primer


<220>
<221>  misc_feature
<222>  (59)..(61)
<223>  n is a, c, g, or t

<400>  119
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tccgatctnn       60

nttgtggaaa ggacgaaaca ccg                                               83


<210>  120
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TREG_NGS_2F Primer


<220>
<221>  misc_feature
<222>  (59)..(62)
<223>  n is a, c, g, or t

<400>  120
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tccgatctnn       60

nnttgtggaa aggacgaaac accg                                              84


<210>  121
<211>  85
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TREG_NGS_2F Primer


<220>
<221>  misc_feature
<222>  (59)..(63)
<223>  n is a, c, g, or t

<400>  121
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tccgatctnn       60

nnnttgtgga aaggacgaaa caccg                                             85


<210>  122
<211>  86
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TREG_NGS_2F Primer


<220>
<221>  misc_feature
<222>  (59)..(64)
<223>  n is a, c, g, or t

<400>  122
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tccgatctnn       60

nnnnttgtgg aaaggacgaa acaccg                                            86


<210>  123
<211>  87
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TREG_NGS_2F Primer


<220>
<221>  misc_feature
<222>  (59)..(65)
<223>  n is a, c, g, or t

<400>  123
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tccgatctnn       60

nnnnnttgtg gaaaggacga aacaccg                                           87


<210>  124
<211>  88
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TREG_NGS_2F Primer


<220>
<221>  misc_feature
<222>  (59)..(66)
<223>  n is a, c, g, or t

<400>  124
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tccgatctnn       60

nnnnnnttgt ggaaaggacg aaacaccg                                          88


