                         SEQUENCE LISTING

<110>  Mammoth Bioscience, Inc.
       WRIGHT, William Douglass
       RAUCH, Benjamin Julius
       DELOUGHERY, Aaron
       BROUGHTON, Janes Paul
 
<120>  ENHANCED GUIDE NUCLEIC ACIDS AND METHODS OF USE

<130>  203477-744601/PCT

<150>  63/187,864
<151>  2021-05-12

<150>  63/213,641
<151>  2021-06-22

<160>  106   

<170>  PatentIn version 3.5

<210>  1
<211>  529
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas14 protein

<400>  1

Met Ala Lys Asn Thr Ile Thr Lys Thr Leu Lys Leu Arg Ile Val Arg 
1               5                   10                  15      


Pro Tyr Asn Ser Ala Glu Val Glu Lys Ile Val Ala Asp Glu Lys Asn 
            20                  25                  30          


Asn Arg Glu Lys Ile Ala Leu Glu Lys Asn Lys Asp Lys Val Lys Glu 
        35                  40                  45              


Ala Cys Ser Lys His Leu Lys Val Ala Ala Tyr Cys Thr Thr Gln Val 
    50                  55                  60                  


Glu Arg Asn Ala Cys Leu Phe Cys Lys Ala Arg Lys Leu Asp Asp Lys 
65                  70                  75                  80  


Phe Tyr Gln Lys Leu Arg Gly Gln Phe Pro Asp Ala Val Phe Trp Gln 
                85                  90                  95      


Glu Ile Ser Glu Ile Phe Arg Gln Leu Gln Lys Gln Ala Ala Glu Ile 
            100                 105                 110         


Tyr Asn Gln Ser Leu Ile Glu Leu Tyr Tyr Glu Ile Phe Ile Lys Gly 
        115                 120                 125             


Lys Gly Ile Ala Asn Ala Ser Ser Val Glu His Tyr Leu Ser Asp Val 
    130                 135                 140                 


Cys Tyr Thr Arg Ala Ala Glu Leu Phe Lys Asn Ala Ala Ile Ala Ser 
145                 150                 155                 160 


Gly Leu Arg Ser Lys Ile Lys Ser Asn Phe Arg Leu Lys Glu Leu Lys 
                165                 170                 175     


Asn Met Lys Ser Gly Leu Pro Thr Thr Lys Ser Asp Asn Phe Pro Ile 
            180                 185                 190         


Pro Leu Val Lys Gln Lys Gly Gly Gln Tyr Thr Gly Phe Glu Ile Ser 
        195                 200                 205             


Asn His Asn Ser Asp Phe Ile Ile Lys Ile Pro Phe Gly Arg Trp Gln 
    210                 215                 220                 


Val Lys Lys Glu Ile Asp Lys Tyr Arg Pro Trp Glu Lys Phe Asp Phe 
225                 230                 235                 240 


Glu Gln Val Gln Lys Ser Pro Lys Pro Ile Ser Leu Leu Leu Ser Thr 
                245                 250                 255     


Gln Arg Arg Lys Arg Asn Lys Gly Trp Ser Lys Asp Glu Gly Thr Glu 
            260                 265                 270         


Ala Glu Ile Lys Lys Val Met Asn Gly Asp Tyr Gln Thr Ser Tyr Ile 
        275                 280                 285             


Glu Val Lys Arg Gly Ser Lys Ile Gly Glu Lys Ser Ala Trp Met Leu 
    290                 295                 300                 


Asn Leu Ser Ile Asp Val Pro Lys Ile Asp Lys Gly Val Asp Pro Ser 
305                 310                 315                 320 


Ile Ile Gly Gly Ile Asp Val Gly Val Lys Ser Pro Leu Val Cys Ala 
                325                 330                 335     


Ile Asn Asn Ala Phe Ser Arg Tyr Ser Ile Ser Asp Asn Asp Leu Phe 
            340                 345                 350         


His Phe Asn Lys Lys Met Phe Ala Arg Arg Arg Ile Leu Leu Lys Lys 
        355                 360                 365             


Asn Arg His Lys Arg Ala Gly His Gly Ala Lys Asn Lys Leu Lys Pro 
    370                 375                 380                 


Ile Thr Ile Leu Thr Glu Lys Ser Glu Arg Phe Arg Lys Lys Leu Ile 
385                 390                 395                 400 


Glu Arg Trp Ala Cys Glu Ile Ala Asp Phe Phe Ile Lys Asn Lys Val 
                405                 410                 415     


Gly Thr Val Gln Met Glu Asn Leu Glu Ser Met Lys Arg Lys Glu Asp 
            420                 425                 430         


Ser Tyr Phe Asn Ile Arg Leu Arg Gly Phe Trp Pro Tyr Ala Glu Met 
        435                 440                 445             


Gln Asn Lys Ile Glu Phe Lys Leu Lys Gln Tyr Gly Ile Glu Ile Arg 
    450                 455                 460                 


Lys Val Ala Pro Asn Asn Thr Ser Lys Thr Cys Ser Lys Cys Gly His 
465                 470                 475                 480 


Leu Asn Asn Tyr Phe Asn Phe Glu Tyr Arg Lys Lys Asn Lys Phe Pro 
                485                 490                 495     


His Phe Lys Cys Glu Lys Cys Asn Phe Lys Glu Asn Ala Asp Tyr Asn 
            500                 505                 510         


Ala Ala Leu Asn Ile Ser Asn Pro Lys Leu Lys Ser Thr Lys Glu Glu 
        515                 520                 525             


Pro 
    


<210>  2
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nuclear localization signal

<400>  2

Lys Arg Pro Ala Ala Thr Lys Lys Ala Gly Gln Ala Lys Lys Lys Lys 
1               5                   10                  15      


Glu Phe 
        


<210>  3
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Endosomal escape protein


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is independently selected from lysine, histidine, and 
       arginine

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa is independently selected from lysine, histidine, and 
       arginine

<220>
<221>  MISC_FEATURE
<222>  (11)..(11)
<223>  Xaa is independently selected from lysine, histidine, and 
       arginine

<220>
<221>  MISC_FEATURE
<222>  (15)..(15)
<223>  Xaa is independently selected from lysine, histidine, and 
       arginine

<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  Xaa is independently selected from lysine, histidine, and 
       arginine

<400>  3

Gly Leu Phe Xaa Ala Leu Leu Xaa Leu Leu Xaa Ser Leu Trp Xaa Leu 
1               5                   10                  15      


Leu Leu Xaa Ala 
            20  


<210>  4
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Endosomal escape protein

<400>  4

Gly Leu Phe His Ala Leu Leu His Leu Leu His Ser Leu Trp His Leu 
1               5                   10                  15      


Leu Leu His Ala 
            20  


<210>  5
<211>  0
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker protein

<400>  5
000
<210>  6
<211>  0
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker protein

<400>  6
000
<210>  7
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker protein


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  S is present in 'n' number of copies wherein n is an integer of 
       at least one

<400>  7

Gly Ser Gly Gly Ser 
1               5   


<210>  8
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker protein


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  S is present in 'n' number of copies wherein n is an integer of 
       at least one

<400>  8

Gly Gly Ser Gly Gly Ser 
1               5       


<210>  9
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker protein


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  S is present in 'n' number of copies wherein n is an integer of 
       at least one

<400>  9

Gly Gly Gly Ser 
1               


<210>  10
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker protein

<400>  10

Gly Gly Ser Gly 
1               


<210>  11
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker protein

<400>  11

Gly Gly Ser Gly Gly 
1               5   


<210>  12
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker protein

<400>  12

Gly Ser Gly Ser Gly 
1               5   


<210>  13
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker protein

<400>  13

Gly Ser Gly Gly Gly 
1               5   


<210>  14
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker protein

<400>  14

Gly Gly Gly Ser Gly 
1               5   


<210>  15
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker protein

<400>  15

Gly Ser Ser Ser Gly 
1               5   


<210>  16
<211>  140
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  R1518 (control)

<400>  16
cuucacugau aaaguggaga accgcuucac caaaagcugu cccuuagggg auuagaacuu       60

gagugaaggu gggcugcuug caucagccua augucgagaa gugcuuucuu cggaaaguaa      120

cccucgaaac aaauucauuu                                                  140


<210>  17
<211>  140
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R5162

<400>  17
cuucacugau aaaguggaga accgcuucac caagugcugu cccuuagggg auuagcacuu       60

gagugaaggu gggcugcuug caucagccua augucgagaa gugcuuucuu cggaaaguaa      120

cccucgaaac aaauucauuu                                                  140


<210>  18
<211>  116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R5163

<400>  18
cuucaccaag ugcugucccu uaggggauua gcacuugagu gaaggugggc ugcuugcauc       60

agccuaaugu cgagaagugc uuucuucgga aaguaacccu cgaaacaaau ucauuu          116


<210>  19
<211>  120
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R5165

<400>  19
accgcuucac caagugcugu cccuuagggg auuagcacuu gagugaaggu gggcugcuug       60

caucagccua augucgagaa gugcuuucuu cggaaaguaa cccucgaaac aaauucauuu      120


<210>  20
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  TracrRNA

<400>  20
cuucacugau aaaguggag                                                    19


<210>  21
<211>  121
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  TracrRNA

<400>  21
aaccgcuuca ccaaaagcug ucccuuaggg gauuagaacu ugagugaagg ugggcugcuu       60

gcaucagccu aaugucgaga agugcuuucu ucggaaagua acccucgaaa caaauucauu      120

u                                                                      121


<210>  22
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PAM sequence

<400>  22
000

<210>  23
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PAM sequence

<400>  23
000

<210>  24
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PAM sequence

<400>  24
000

<210>  25
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PAM sequence

<400>  25
000

<210>  26
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PAM sequence

<400>  26
000

<210>  27
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PAM sequence

<400>  27
000

<210>  28
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PAM sequence

<400>  28
000

<210>  29
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PAM sequence

<400>  29
000

<210>  30
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PAM sequence

<400>  30
000

<210>  31
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PAM sequence

<400>  31
000

<210>  32
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PAM sequence

<400>  32
000

<210>  33
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PAM sequence

<400>  33
000

<210>  34
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PAM sequence

<400>  34
000

<210>  35
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PAM sequence

<400>  35
000

<210>  36
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PAM sequence

<400>  36
000

<210>  37
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PAM sequence

<400>  37
000

<210>  38
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PAM sequence

<400>  38
000

<210>  39
<211>  0
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PAM sequence

<400>  39
000

<210>  40
<211>  159
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas14a.1 nuclease and a sgRNA (R6104)

<400>  40
ccgcuucacc aagugcuguc ccuuagggga uuagcacuug agugaaggug ggcugcuugc       60

aucagccuaa ugucgagaag ugcuuucuuc ggaaaguaac ccucgaaaca aauucauuga      120

aagaaugaag gaaugcaacu cuugcccggg cucaaccuu                             159


<210>  41
<211>  118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Modified Cas14a.1 tracrRNA

<400>  41
ccgcuucacc aagugcuguc ccuuagggga uuagcacuug agugaaggug ggcugcuugc       60

aucagccuaa ugucgagaag ugcuuucuuc ggaaaguaac ccucgaaaca aauucauu        118


<210>  42
<211>  120
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas14a.1 tracrRNA

<400>  42
accgcuucac caagugcugu cccuuagggg auuagcacuu gagugaaggu gggcugcuug       60

caucagccua augucgagaa gugcuuucuu cggaaaguaa cccucgaaac aaauucauuu      120


<210>  43
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Repeat sequence

<400>  43
gaaagaauga aggaaugcaa c                                                 21


<210>  44
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  44
gaaagaauga aggaaugcaa c                                                 21


<210>  45
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  45
ucuugcccgg gcucaaccuu                                                   20


<210>  46
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Spacer sequence

<400>  46
uauuaaauac ucguauugcu                                                   20


<210>  47
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Acrydite-modified Reporter 172

<400>  47
tttttttttt tttttttttt                                                   20


<210>  48
<211>  150
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA

<400>  48
uucuaaaucu gcagaggaua aguuggcgcu ucaccaaaag cugucccuua ggggauuaga       60

acuugaguga agggcugauu ucaacagcca aauaucgagg ugccggaauu guuaaauaau      120

cuccggagua cacccucgaa acaauuuaau                                       150


<210>  49
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  49
gucugaauca caauuccauu aaaacaagga uugaaac                                37


<210>  50
<211>  89
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA

<400>  50
cgccgccaga aaugagcggc ggugggcugc uugcaucagc cuaaugucga gaagugcgcg       60

cuucggcgcg uaacccucga aacaaauuc                                         89


<210>  51
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  51
gaaugaagga augcaac                                                      17


<210>  52
<211>  119
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA

<400>  52
uucuaaaucu gcagaggaua aguuggcgua gaugccggca uugggcugcu cccaagcauc       60

agccuaaugu cgagaagugc uuucuucgga aaguaacccu cgaaacaaau ucauuuuuc       119


<210>  53
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  53
guugcagaac ccgaauagac gaaugaagga uugggac                                37


<210>  54
<211>  91
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA

<400>  54
cgccgccaag aaauugagcg gcggugggcu gcuugcauca gccuaauguc gagaagugcg       60

cgcuucggcg cguaacccuc gaaacaaauu c                                      91


<210>  55
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  55
gcgcgaagga augcaac                                                      17


<210>  56
<211>  129
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA

<400>  56
uucuaaaucu gcagaggaua aguuggcgua gaugccauuc gaaagaggca uugggcugcu       60

cccaagcauc agccuaaugu cgagaagugc uuucuucgga aaguaacccu cgaaacaaau      120

ucauuuuuc                                                              129


<210>  57
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  57
guugcagaac ccgaauagac gaaugaagga uugggac                                37


<210>  58
<211>  113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA

<400>  58
gcguagaugc cauucaacaa aucggaaaga ggcauggcug auuucaacag ccaaauaucg       60

aggugccgga auuguuaaau aaucuccgga guacacccuc gaaacaauuu aau             113


<210>  59
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  59
gucugaauca caauuccauu aaaacaagga uugaaac                                37


<210>  60
<211>  141
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA

<400>  60
gcuucacuga uaaaguggag aaccgcuuca ccaaaagcug ucccuuaggg gauuagaacu       60

ugagugaagg ugggcugcuu gcaucagccu aaugucgaga agugcuuucu ucggaaagua      120

acccucgaaa caaauucauu u                                                141


<210>  61
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  61
gaaugaagga augcaac                                                      17


<210>  62
<211>  163
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA

<400>  62
cuucacugau aaaguggaga accguagaug ccauucgaaa gaggcaugug ggcugcuugc       60

aucagccuaa ugucgagaag ugcuuucuuc ggaaaguaac ccucgaaaca aauucauuug      120

aagaaagaau gaaggaaugc aacuauuaaa uacucguauu gcu                        163


<210>  63
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  63
gaaugaagga augcaac                                                      17


<210>  64
<211>  138
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA

<400>  64
uucuaaaucu gcagaggaua aguuggcgua gaugccauuc aacaaaucgg aaagaggcau       60

ggcugauuuc aacagccaaa uaucgaggug ccggaauugu uaaauaaucu ccggaguaca      120

cccucgaaac aauuuaau                                                    138


<210>  65
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  65
gucugaauca caauuccauu aaaacaagga uugaaac                                37


<210>  66
<211>  138
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA

<400>  66
uucuaaaucu gcagaggaua aguuggcgua ggcgccagcc aacaaaucgg gcagaggcgc       60

ggcugauuuc aacagccaaa uaucgaggug ccggaauugu uaaauaaucu ccggaguaca      120

cccucgaaac aauuuaau                                                    138


<210>  67
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  67
gucugaauca caauuccauu aaaacaagga uugaaac                                37


<210>  68
<211>  140
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA

<400>  68
cuucacugau aaaguggaga accgcuucac caagugcugu cccuuagggg auuagcacuu       60

gagugaaggu gggcugcuug caucagccua augucgagaa gugcuuucuu cggaaaguaa      120

cccucgaaac aaauucauuu                                                  140


<210>  69
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  69
gaaugaagga augcaac                                                      17


<210>  70
<211>  129
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA

<400>  70
uucuaaaucu gcagaggaua aguuggcgua gaugccauuc gaaagaggca uggcugauuu       60

caacagccaa auaucgaggu gccggaauug uuaaauaauc uccggaguac acccucgaaa      120

caauuuaau                                                              129


<210>  71
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  71
gucugaauca caauuccauu aaaacaagga uugaaac                                37


<210>  72
<211>  131
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA

<400>  72
uucuaaaucu gcagaggaua aguuggcgua ggcgccagcc aacaaaucgg gcagaggcgc       60

ugggcugcuu gcaucagccu aaugucgaga agugcuuucu ucggaaagua acccucgaaa      120

caaauucauu u                                                           131


<210>  73
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  73
gaaugaagga augcaac                                                      17


<210>  74
<211>  119
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA

<400>  74
uucuaaaucu gcagaggaua aguuggcgua gaugccggca uggcugauuu caacagccaa       60

auaucgaggu gccggaauug uuaaauaauc uccggaguac acccucgaaa caauuuaau       119


<210>  75
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  75
gucugaauca caauuccauu aaaacaagga uugaaac                                37


<210>  76
<211>  138
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA

<400>  76
uucuaaaucu gcagaggaua aguuggcgua ggcgccagcc aacaaaucgg gcagaggcgc       60

ugggcugcuc ccaagcauca gccuaauguc gagaagugcu uucuucggaa aguaacccuc      120

gaaacaaauu cauuuuuc                                                    138


<210>  77
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  77
guugcagaac ccgaauagac gaaugaagga uugggac                                37


<210>  78
<211>  89
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA

<400>  78
cuucaccaga aaugagugaa ggugggcugc uugcaucagc cuaaugucga gaagugcuuu       60

cuucggaaag uaacccucga aacaaauuc                                         89


<210>  79
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  79
gaaugaagga augcaac                                                      17


<210>  80
<211>  132
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA

<400>  80
uucuaaaucu gcagaggaua aguuggcgua gaugccauuc aacaaaucgg aaagaggcau       60

gugggcugcu ugcaucagcc uaaugucgag aagugcuuuc uucggaaagu aacccucgaa      120

acaaauucau uu                                                          132


<210>  81
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  81
gaaugaagga augcaac                                                      17


<210>  82
<211>  138
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA

<400>  82
uucuaaaucu gcagaggaua aguuggcgua ggcgccauuc aacaaaucgg aaagaggcgc       60

ggcugauuuc aacagccaaa uaucgaggug ccggaauugu uaaauaaucu ccggaguaca      120

cccucgaaac aauuuaau                                                    138


<210>  83
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  83
gucugaauca caauuccauu aaaacaagga uugaaac                                37


<210>  84
<211>  91
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA

<400>  84
cuucaccaag aaauugagug aaggugggcu gcuugcauca gccuaauguc gagaagugcu       60

uucuucggaa aguaacccuc gaaacaaauu c                                      91


<210>  85
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  85
gaaugaagga augcaac                                                      17


<210>  86
<211>  143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  86
cuucacugau aaaguggaga accguagaug ccauucgaaa gaggcaugug ggcugcuugc       60

aucagccuaa ugucgagaag ugcuuucuuc ggaaaguaac ccucgaaaca aauucauuug      120

aagaaagaau gaaggaaugc aac                                              143


<210>  87
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  87
gaaugaagga augcaac                                                      17


<210>  88
<211>  122
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  88
cuucacugau aaaguggaga accguagaug ccauucgaaa gaggcaugug ggcugcuugc       60

aucagccuaa ugucgagaag ugcuuucuuc ggaaaguaac ccucgaaaca aauucauuug      120

aa                                                                     122


<210>  89
<211>  113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  89
gcgccgccaa gaaauugagc ggcggugggc ugcuugcauc agccuaaugu cgagaagugc       60

gcgcuucggc gcguaacccu cgaaacaagc gcgaaagaau gaaggaaugc aac             113


<210>  90
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  90
gaaugaagga augcaac                                                      17


<210>  91
<211>  92
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  91
gcgccgccaa gaaauugagc ggcggugggc ugcuugcauc agccuaaugu cgagaagugc       60

gcgcuucggc gcguaacccu cgaaacaagc gc                                     92


<210>  92
<211>  111
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  92
gcgccgccag aaaugagcgg cggugggcug cuugcaucag ccuaaugucg agaagugcgc       60

gcuucggcgc guaacccucg aaacaagcgc gaaagaauga aggaaugcaa c               111


<210>  93
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  93
gaaugaagga augcaac                                                      17


<210>  94
<211>  90
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  94
gcgccgccag aaaugagcgg cggugggcug cuugcaucag ccuaaugucg agaagugcgc       60

gcuucggcgc guaacccucg aaacaagcgc                                        90


<210>  95
<211>  113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  95
gcuucaccaa gaaauugagu gaaggugggc ugcuugcauc agccuaaugu cgagaagugc       60

uuucuucgga aaguaacccu cgaaacaaau ucgaaagaau gaaggaaugc aac             113


<210>  96
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  96
gaaugaagga augcaac                                                      17


<210>  97
<211>  92
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  97
gcuucaccaa gaaauugagu gaaggugggc ugcuugcauc agccuaaugu cgagaagugc       60

uuucuucgga aaguaacccu cgaaacaaau uc                                     92


<210>  98
<211>  111
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  98
gcuucaccag aaaugaguga aggugggcug cuugcaucag ccuaaugucg agaagugcuu       60

ucuucggaaa guaacccucg aaacaaauuc gaaagaauga aggaaugcaa c               111


<210>  99
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  99
gaaugaagga augcaac                                                      17


<210>  100
<211>  90
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  100
gcuucaccag aaaugaguga aggugggcug cuugcaucag ccuaaugucg agaagugcuu       60

ucuucggaaa guaacccucg aaacaaauuc                                        90


<210>  101
<211>  156
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  101
uucuaaaucu gcagaggaua aguuggcgua gaugccauuc aacaaaucgg aaagaggcau       60

gugggcugcu ugcaucagcc uaaugucgag aagugcuuuc uucggaaagu aacccucgaa      120

acaaauucau uugaagaaag aaugaaggaa ugcaac                                156


<210>  102
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  102
gaaugaagga augcaac                                                      17


<210>  103
<211>  135
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  103
uucuaaaucu gcagaggaua aguuggcgua gaugccauuc aacaaaucgg aaagaggcau       60

gugggcugcu ugcaucagcc uaaugucgag aagugcuuuc uucggaaagu aacccucgaa      120

acaaauucau uugaa                                                       135


<210>  104
<211>  153
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  104
uucuaaaucu gcagaggaua aguuggcgua gaugccauuc gaaagaggca uugggcugcu       60

cccaagcauc agccuaaugu cgagaagugc uuucuucgga aaguaacccu cgaaacaaau      120

ucauuuuucg aagaaagaau gaaggaaugc aac                                   153


<210>  105
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  105
gaaugaagga augcaac                                                      17


<210>  106
<211>  132
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  106
uucuaaaucu gcagaggaua aguuggcgua gaugccauuc gaaagaggca uugggcugcu       60

cccaagcauc agccuaaugu cgagaagugc uuucuucgga aaguaacccu cgaaacaaau      120

ucauuuuucg aa                                                          132


