<110>    CJ CheilJedang Corporation

<120>    MICROORGANISM HAVING INHANCED ACTIVITY OF 3-METHYL-2-OXOBUTANOATE
         HYDROXYMETHYLTRANSFERASE AND USES THEREOF

<130>    OPP20220698KR

<150>    KR 10-2021-0060009
<151>    2021-05-10

<160>    128

<170>    koPatentIn 3.0

<210>    1
<211>    37
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    1
tagatcgaaa ggtgcacaaa gatgaaaccg accacca                                  37


<210>    2
<211>    41
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    2
ccgctctaga actagtggat cttaatggaa actgtgttct t                             41


<210>    3
<211>    36
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    3
tagatcgaaa ggtgcacaaa gatgaaaccg accacc                                   36


<210>    4
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    4
ccgctctaga actagtggat cttaatggaa actgtgttct                               40


<210>    5
<211>    38
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    5
tagatcgaaa ggtgcacaaa gatgaaacca accaccat                                 38


<210>    6
<211>    41
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    6
ccgctctaga actagtggat cttaatggaa actgtgttct t                             41


<210>    7
<211>    42
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    7
tagatcgaaa ggtgcacaaa gatgtcatta aagcaaataa ct                            42


<210>    8
<211>    41
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    8
ccgctctaga actagtggat cttattggaa actgtgttct t                             41


<210>    9
<211>    36
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    9
tagatcgaaa ggtgcacaaa gatgaaaccc accacc                                   36


<210>    10
<211>    39
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    10
ccgctctaga actagtggat cttagttaaa tgagtgctc                                39


<210>    11
<211>    37
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    11
tagatcgaaa ggtgcacaaa gatgaaaccg accacca                                  37


<210>    12
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    12
ccgctctaga actagtggat cttaatggaa actgtgttct                               40


<210>    13
<211>    36
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    13
tagatcgaaa ggtgcacaaa gatgaaaccc accacg                                   36


<210>    14
<211>    38
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    14
ccgctctaga actagtggat cttactggaa actgtgct                                 38


<210>    15
<211>    44
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    15
tagatcgaaa ggtgcacaaa gatgaaaact ttaaatcatt taaa                          44


<210>    16
<211>    39
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    16
ccgctctaga actagtggat cttagtcatg ttgctctac                                39


<210>    17
<211>    37
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    17
tagatcgaaa ggtgcacaaa gatgccagat gtaaccg                                  37


<210>    18
<211>    36
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    18
ccgctctaga actagtggat ctcatgcgga gaaccc                                   36


<210>    19
<211>    36
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    19
tagatcgaaa ggtgcacaaa gatgcctgat gtgacc                                   36


<210>    20
<211>    36
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    20
ccgctctaga actagtggat ctcatgcgga gaatcc                                   36


<210>    21
<211>    39
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    21
tagatcgaaa ggtgcacaaa gatgtcggta cataaagaa                                39


<210>    22
<211>    42
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    22
ccgctctaga actagtggat cttaatattg ttctttttcg tt                            42


<210>    23
<211>    36
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    23
tagatcgaaa ggtgcacaaa gatgaaaccc accacc                                   36


<210>    24
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    24
ccgctctaga actagtggat cttagttgaa agagtgctct                               40


<210>    25
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    25
tagatcgaaa ggtgcacaaa gatgtctaca gcaaaaaaag                               40


<210>    26
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    26
ccgctctaga actagtggat cttaatactg ttcatcgtca                               40


<210>    27
<211>    41
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    27
cttgatatcg aattcctgca ttcagggtag ttgactaaag a                             41


<210>    28
<211>    17
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    28
ctttgtgcac ctttcga                                                        17


<210>    29
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    29
tgaattcgag ctcggtaccc gaaatagcgc ttgatgaatc                               40


<210>    30
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    30
ggttgctacc tgcacccggg gggcatgagt atagatgtga                               40


<210>    31
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    31
ctatactcat gccccccggg tgcaggtagc aaccacaaag                               40


<210>    32
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    32
gtcgactcta gaggatcccc tatgtggcgt tgggtgcagc                               40


<210>    33
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    33
catctatact catgcccccc ttcagggtag ttgactaaag                               40


<210>    34
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    34
tgtggttgct acctgcaccc ttaatggaaa ctgtgttctt                               40


<210>    35
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    35
cccacccggt gtcattcgac                                                     20


<210>    36
<211>    19
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    36
gcgcatccag ctcatcggt                                                      19


<210>    37
<211>    264
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    synthetic_PanB WT


<400>    37
Met Lys Pro Thr Thr Ile Ser Leu Leu Gln Lys Tyr Lys Gln Glu Lys
  1               5                  10                  15 

Lys Arg Phe Ala Thr Ile Thr Ala Tyr Asp Tyr Ser Phe Ala Lys Leu
             20                  25                  30 

Phe Ala Asp Glu Gly Leu Asn Val Met Leu Val Gly Asp Ser Leu Gly
         35                  40                  45 

Met Thr Val Gln Gly His Asp Ser Thr Leu Pro Val Thr Val Ala Asp
     50                  55                  60 

Ile Ala Tyr His Thr Ala Ala Val Arg Arg Gly Ala Pro Asn Cys Leu
 65                  70                  75                  80 

Leu Leu Ala Asp Leu Pro Phe Met Ala Tyr Ala Thr Pro Glu Gln Ala
                 85                  90                  95 

Phe Glu Asn Ala Ala Thr Val Met Arg Ala Gly Ala Asn Met Val Lys
            100                 105                 110 

Ile Glu Gly Gly Glu Trp Leu Val Glu Thr Val Gln Met Leu Thr Glu
        115                 120                 125 

Arg Ala Val Pro Val Cys Gly His Leu Gly Leu Thr Pro Gln Ser Val
    130                 135                 140 

Asn Ile Phe Gly Gly Tyr Lys Val Gln Gly Arg Gly Asp Glu Ala Gly
145                 150                 155                 160 

Asp Gln Leu Leu Ser Asp Ala Leu Ala Leu Glu Ala Ala Gly Ala Gln
                165                 170                 175 

Leu Leu Val Leu Glu Cys Val Pro Val Glu Leu Ala Lys Arg Ile Thr
            180                 185                 190 

Glu Ala Leu Ala Ile Pro Val Ile Gly Ile Gly Ala Gly Asn Val Thr
        195                 200                 205 

Asp Gly Gln Ile Leu Val Met His Asp Ala Phe Gly Ile Thr Gly Gly
    210                 215                 220 

His Ile Pro Lys Phe Ala Lys Asn Phe Leu Ala Glu Thr Gly Asp Ile
225                 230                 235                 240 

Arg Ala Ala Val Arg Gln Tyr Met Ala Glu Val Glu Ser Gly Val Tyr
                245                 250                 255 

Pro Gly Glu Glu His Ser Phe His
            260                


<210>    38
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    38
gtttctacca gccactcgga gccttcaatt ttgaccatgt                               40


<210>    39
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    39
acatggtcaa aattgaaggc tccgagtggc tggtagaaac                               40


<210>    40
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    40
gtttctacca gccactcgca gccttcaatt ttgaccatgt                               40


<210>    41
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    41
acatggtcaa aattgaaggc tgcgagtggc tggtagaaac                               40


<210>    42
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    42
gtttctacca gccactccag gccttcaatt ttgaccatgt                               40


<210>    43
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    43
acatggtcaa aattgaaggc ctggagtggc tggtagaaac                               40


<210>    44
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    44
gtttctacca gccactcgat gccttcaatt ttgaccatgt                               40


<210>    45
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    45
acatggtcaa aattgaaggc atcgagtggc tggtagaaac                               40


<210>    46
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    46
gtttctacca gccactcggt gccttcaatt ttgaccatgt                               40


<210>    47
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    47
acatggtcaa aattgaaggc accgagtggc tggtagaaac                               40


<210>    48
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    48
gtttctacca gccactccac gccttcaatt ttgaccatgt                               40


<210>    49
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    49
acatggtcaa aattgaaggc gtggagtggc tggtagaaac                               40


<210>    50
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    50
gtttctacca gccactccat gccttcaatt ttgaccatgt                               40


<210>    51
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    51
acatggtcaa aattgaaggc atggagtggc tggtagaaac                               40


<210>    52
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    52
gtttctacca gccactcatc gccttcaatt ttgaccatgt                               40


<210>    53
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    53
acatggtcaa aattgaaggc gatgagtggc tggtagaaac                               40


<210>    54
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    54
gtttctacca gccactcttc gccttcaatt ttgaccatgt                               40


<210>    55
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    55
acatggtcaa aattgaaggc gaagagtggc tggtagaaac                               40


<210>    56
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    56
gtttctacca gccactcgtt gccttcaatt ttgaccatgt                               40


<210>    57
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    57
acatggtcaa aattgaaggc aacgagtggc tggtagaaac                               40


<210>    58
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    58
gtttctacca gccactcctg gccttcaatt ttgaccatgt                               40


<210>    59
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    59
acatggtcaa aattgaaggc caggagtggc tggtagaaac                               40


<210>    60
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    60
gtttctacca gccactctgc gccttcaatt ttgaccatgt                               40


<210>    61
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_primer


<400>    61
acatggtcaa aattgaaggc gcagagtggc tggtagaaac                               40


<210>    62
<211>    264
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    synthetic_PanB with G116A mutation


<400>    62
Met Lys Pro Thr Thr Ile Ser Leu Leu Gln Lys Tyr Lys Gln Glu Lys
  1               5                  10                  15 

Lys Arg Phe Ala Thr Ile Thr Ala Tyr Asp Tyr Ser Phe Ala Lys Leu
             20                  25                  30 

Phe Ala Asp Glu Gly Leu Asn Val Met Leu Val Gly Asp Ser Leu Gly
         35                  40                  45 

Met Thr Val Gln Gly His Asp Ser Thr Leu Pro Val Thr Val Ala Asp
     50                  55                  60 

Ile Ala Tyr His Thr Ala Ala Val Arg Arg Gly Ala Pro Asn Cys Leu
 65                  70                  75                  80 

Leu Leu Ala Asp Leu Pro Phe Met Ala Tyr Ala Thr Pro Glu Gln Ala
                 85                  90                  95 

Phe Glu Asn Ala Ala Thr Val Met Arg Ala Gly Ala Asn Met Val Lys
            100                 105                 110 

Ile Glu Gly Ala Glu Trp Leu Val Glu Thr Val Gln Met Leu Thr Glu
        115                 120                 125 

Arg Ala Val Pro Val Cys Gly His Leu Gly Leu Thr Pro Gln Ser Val
    130                 135                 140 

Asn Ile Phe Gly Gly Tyr Lys Val Gln Gly Arg Gly Asp Glu Ala Gly
145                 150                 155                 160 

Asp Gln Leu Leu Ser Asp Ala Leu Ala Leu Glu Ala Ala Gly Ala Gln
                165                 170                 175 

Leu Leu Val Leu Glu Cys Val Pro Val Glu Leu Ala Lys Arg Ile Thr
            180                 185                 190 

Glu Ala Leu Ala Ile Pro Val Ile Gly Ile Gly Ala Gly Asn Val Thr
        195                 200                 205 

Asp Gly Gln Ile Leu Val Met His Asp Ala Phe Gly Ile Thr Gly Gly
    210                 215                 220 

His Ile Pro Lys Phe Ala Lys Asn Phe Leu Ala Glu Thr Gly Asp Ile
225                 230                 235                 240 

Arg Ala Ala Val Arg Gln Tyr Met Ala Glu Val Glu Ser Gly Val Tyr
                245                 250                 255 

Pro Gly Glu Glu His Ser Phe His
            260                


<210>    63
<211>    264
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    synthetic_PanB with G116N mutation


<400>    63
Met Lys Pro Thr Thr Ile Ser Leu Leu Gln Lys Tyr Lys Gln Glu Lys
  1               5                  10                  15 

Lys Arg Phe Ala Thr Ile Thr Ala Tyr Asp Tyr Ser Phe Ala Lys Leu
             20                  25                  30 

Phe Ala Asp Glu Gly Leu Asn Val Met Leu Val Gly Asp Ser Leu Gly
         35                  40                  45 

Met Thr Val Gln Gly His Asp Ser Thr Leu Pro Val Thr Val Ala Asp
     50                  55                  60 

Ile Ala Tyr His Thr Ala Ala Val Arg Arg Gly Ala Pro Asn Cys Leu
 65                  70                  75                  80 

Leu Leu Ala Asp Leu Pro Phe Met Ala Tyr Ala Thr Pro Glu Gln Ala
                 85                  90                  95 

Phe Glu Asn Ala Ala Thr Val Met Arg Ala Gly Ala Asn Met Val Lys
            100                 105                 110 

Ile Glu Gly Asn Glu Trp Leu Val Glu Thr Val Gln Met Leu Thr Glu
        115                 120                 125 

Arg Ala Val Pro Val Cys Gly His Leu Gly Leu Thr Pro Gln Ser Val
    130                 135                 140 

Asn Ile Phe Gly Gly Tyr Lys Val Gln Gly Arg Gly Asp Glu Ala Gly
145                 150                 155                 160 

Asp Gln Leu Leu Ser Asp Ala Leu Ala Leu Glu Ala Ala Gly Ala Gln
                165                 170                 175 

Leu Leu Val Leu Glu Cys Val Pro Val Glu Leu Ala Lys Arg Ile Thr
            180                 185                 190 

Glu Ala Leu Ala Ile Pro Val Ile Gly Ile Gly Ala Gly Asn Val Thr
        195                 200                 205 

Asp Gly Gln Ile Leu Val Met His Asp Ala Phe Gly Ile Thr Gly Gly
    210                 215                 220 

His Ile Pro Lys Phe Ala Lys Asn Phe Leu Ala Glu Thr Gly Asp Ile
225                 230                 235                 240 

Arg Ala Ala Val Arg Gln Tyr Met Ala Glu Val Glu Ser Gly Val Tyr
                245                 250                 255 

Pro Gly Glu Glu His Ser Phe His
            260                


<210>    64
<211>    264
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    synthetic_PanB with G116T mutation


<400>    64
Met Lys Pro Thr Thr Ile Ser Leu Leu Gln Lys Tyr Lys Gln Glu Lys
  1               5                  10                  15 

Lys Arg Phe Ala Thr Ile Thr Ala Tyr Asp Tyr Ser Phe Ala Lys Leu
             20                  25                  30 

Phe Ala Asp Glu Gly Leu Asn Val Met Leu Val Gly Asp Ser Leu Gly
         35                  40                  45 

Met Thr Val Gln Gly His Asp Ser Thr Leu Pro Val Thr Val Ala Asp
     50                  55                  60 

Ile Ala Tyr His Thr Ala Ala Val Arg Arg Gly Ala Pro Asn Cys Leu
 65                  70                  75                  80 

Leu Leu Ala Asp Leu Pro Phe Met Ala Tyr Ala Thr Pro Glu Gln Ala
                 85                  90                  95 

Phe Glu Asn Ala Ala Thr Val Met Arg Ala Gly Ala Asn Met Val Lys
            100                 105                 110 

Ile Glu Gly Thr Glu Trp Leu Val Glu Thr Val Gln Met Leu Thr Glu
        115                 120                 125 

Arg Ala Val Pro Val Cys Gly His Leu Gly Leu Thr Pro Gln Ser Val
    130                 135                 140 

Asn Ile Phe Gly Gly Tyr Lys Val Gln Gly Arg Gly Asp Glu Ala Gly
145                 150                 155                 160 

Asp Gln Leu Leu Ser Asp Ala Leu Ala Leu Glu Ala Ala Gly Ala Gln
                165                 170                 175 

Leu Leu Val Leu Glu Cys Val Pro Val Glu Leu Ala Lys Arg Ile Thr
            180                 185                 190 

Glu Ala Leu Ala Ile Pro Val Ile Gly Ile Gly Ala Gly Asn Val Thr
        195                 200                 205 

Asp Gly Gln Ile Leu Val Met His Asp Ala Phe Gly Ile Thr Gly Gly
    210                 215                 220 

His Ile Pro Lys Phe Ala Lys Asn Phe Leu Ala Glu Thr Gly Asp Ile
225                 230                 235                 240 

Arg Ala Ala Val Arg Gln Tyr Met Ala Glu Val Glu Ser Gly Val Tyr
                245                 250                 255 

Pro Gly Glu Glu His Ser Phe His
            260                


<210>    65
<211>    264
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    synthetic_PanB with G116E mutation


<400>    65
Met Lys Pro Thr Thr Ile Ser Leu Leu Gln Lys Tyr Lys Gln Glu Lys
  1               5                  10                  15 

Lys Arg Phe Ala Thr Ile Thr Ala Tyr Asp Tyr Ser Phe Ala Lys Leu
             20                  25                  30 

Phe Ala Asp Glu Gly Leu Asn Val Met Leu Val Gly Asp Ser Leu Gly
         35                  40                  45 

Met Thr Val Gln Gly His Asp Ser Thr Leu Pro Val Thr Val Ala Asp
     50                  55                  60 

Ile Ala Tyr His Thr Ala Ala Val Arg Arg Gly Ala Pro Asn Cys Leu
 65                  70                  75                  80 

Leu Leu Ala Asp Leu Pro Phe Met Ala Tyr Ala Thr Pro Glu Gln Ala
                 85                  90                  95 

Phe Glu Asn Ala Ala Thr Val Met Arg Ala Gly Ala Asn Met Val Lys
            100                 105                 110 

Ile Glu Gly Glu Glu Trp Leu Val Glu Thr Val Gln Met Leu Thr Glu
        115                 120                 125 

Arg Ala Val Pro Val Cys Gly His Leu Gly Leu Thr Pro Gln Ser Val
    130                 135                 140 

Asn Ile Phe Gly Gly Tyr Lys Val Gln Gly Arg Gly Asp Glu Ala Gly
145                 150                 155                 160 

Asp Gln Leu Leu Ser Asp Ala Leu Ala Leu Glu Ala Ala Gly Ala Gln
                165                 170                 175 

Leu Leu Val Leu Glu Cys Val Pro Val Glu Leu Ala Lys Arg Ile Thr
            180                 185                 190 

Glu Ala Leu Ala Ile Pro Val Ile Gly Ile Gly Ala Gly Asn Val Thr
        195                 200                 205 

Asp Gly Gln Ile Leu Val Met His Asp Ala Phe Gly Ile Thr Gly Gly
    210                 215                 220 

His Ile Pro Lys Phe Ala Lys Asn Phe Leu Ala Glu Thr Gly Asp Ile
225                 230                 235                 240 

Arg Ala Ala Val Arg Gln Tyr Met Ala Glu Val Glu Ser Gly Val Tyr
                245                 250                 255 

Pro Gly Glu Glu His Ser Phe His
            260                


<210>    66
<211>    264
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    synthetic_PanB with G116S mutation


<400>    66
Met Lys Pro Thr Thr Ile Ser Leu Leu Gln Lys Tyr Lys Gln Glu Lys
  1               5                  10                  15 

Lys Arg Phe Ala Thr Ile Thr Ala Tyr Asp Tyr Ser Phe Ala Lys Leu
             20                  25                  30 

Phe Ala Asp Glu Gly Leu Asn Val Met Leu Val Gly Asp Ser Leu Gly
         35                  40                  45 

Met Thr Val Gln Gly His Asp Ser Thr Leu Pro Val Thr Val Ala Asp
     50                  55                  60 

Ile Ala Tyr His Thr Ala Ala Val Arg Arg Gly Ala Pro Asn Cys Leu
 65                  70                  75                  80 

Leu Leu Ala Asp Leu Pro Phe Met Ala Tyr Ala Thr Pro Glu Gln Ala
                 85                  90                  95 

Phe Glu Asn Ala Ala Thr Val Met Arg Ala Gly Ala Asn Met Val Lys
            100                 105                 110 

Ile Glu Gly Ser Glu Trp Leu Val Glu Thr Val Gln Met Leu Thr Glu
        115                 120                 125 

Arg Ala Val Pro Val Cys Gly His Leu Gly Leu Thr Pro Gln Ser Val
    130                 135                 140 

Asn Ile Phe Gly Gly Tyr Lys Val Gln Gly Arg Gly Asp Glu Ala Gly
145                 150                 155                 160 

Asp Gln Leu Leu Ser Asp Ala Leu Ala Leu Glu Ala Ala Gly Ala Gln
                165                 170                 175 

Leu Leu Val Leu Glu Cys Val Pro Val Glu Leu Ala Lys Arg Ile Thr
            180                 185                 190 

Glu Ala Leu Ala Ile Pro Val Ile Gly Ile Gly Ala Gly Asn Val Thr
        195                 200                 205 

Asp Gly Gln Ile Leu Val Met His Asp Ala Phe Gly Ile Thr Gly Gly
    210                 215                 220 

His Ile Pro Lys Phe Ala Lys Asn Phe Leu Ala Glu Thr Gly Asp Ile
225                 230                 235                 240 

Arg Ala Ala Val Arg Gln Tyr Met Ala Glu Val Glu Ser Gly Val Tyr
                245                 250                 255 

Pro Gly Glu Glu His Ser Phe His
            260                


<210>    67
<211>    264
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    synthetic_PanB with G116V mutation


<400>    67
Met Lys Pro Thr Thr Ile Ser Leu Leu Gln Lys Tyr Lys Gln Glu Lys
  1               5                  10                  15 

Lys Arg Phe Ala Thr Ile Thr Ala Tyr Asp Tyr Ser Phe Ala Lys Leu
             20                  25                  30 

Phe Ala Asp Glu Gly Leu Asn Val Met Leu Val Gly Asp Ser Leu Gly
         35                  40                  45 

Met Thr Val Gln Gly His Asp Ser Thr Leu Pro Val Thr Val Ala Asp
     50                  55                  60 

Ile Ala Tyr His Thr Ala Ala Val Arg Arg Gly Ala Pro Asn Cys Leu
 65                  70                  75                  80 

Leu Leu Ala Asp Leu Pro Phe Met Ala Tyr Ala Thr Pro Glu Gln Ala
                 85                  90                  95 

Phe Glu Asn Ala Ala Thr Val Met Arg Ala Gly Ala Asn Met Val Lys
            100                 105                 110 

Ile Glu Gly Val Glu Trp Leu Val Glu Thr Val Gln Met Leu Thr Glu
        115                 120                 125 

Arg Ala Val Pro Val Cys Gly His Leu Gly Leu Thr Pro Gln Ser Val
    130                 135                 140 

Asn Ile Phe Gly Gly Tyr Lys Val Gln Gly Arg Gly Asp Glu Ala Gly
145                 150                 155                 160 

Asp Gln Leu Leu Ser Asp Ala Leu Ala Leu Glu Ala Ala Gly Ala Gln
                165                 170                 175 

Leu Leu Val Leu Glu Cys Val Pro Val Glu Leu Ala Lys Arg Ile Thr
            180                 185                 190 

Glu Ala Leu Ala Ile Pro Val Ile Gly Ile Gly Ala Gly Asn Val Thr
        195                 200                 205 

Asp Gly Gln Ile Leu Val Met His Asp Ala Phe Gly Ile Thr Gly Gly
    210                 215                 220 

His Ile Pro Lys Phe Ala Lys Asn Phe Leu Ala Glu Thr Gly Asp Ile
225                 230                 235                 240 

Arg Ala Ala Val Arg Gln Tyr Met Ala Glu Val Glu Ser Gly Val Tyr
                245                 250                 255 

Pro Gly Glu Glu His Ser Phe His
            260                


<210>    68
<211>    264
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    synthetic_PanB with G116I mutation


<400>    68
Met Lys Pro Thr Thr Ile Ser Leu Leu Gln Lys Tyr Lys Gln Glu Lys
  1               5                  10                  15 

Lys Arg Phe Ala Thr Ile Thr Ala Tyr Asp Tyr Ser Phe Ala Lys Leu
             20                  25                  30 

Phe Ala Asp Glu Gly Leu Asn Val Met Leu Val Gly Asp Ser Leu Gly
         35                  40                  45 

Met Thr Val Gln Gly His Asp Ser Thr Leu Pro Val Thr Val Ala Asp
     50                  55                  60 

Ile Ala Tyr His Thr Ala Ala Val Arg Arg Gly Ala Pro Asn Cys Leu
 65                  70                  75                  80 

Leu Leu Ala Asp Leu Pro Phe Met Ala Tyr Ala Thr Pro Glu Gln Ala
                 85                  90                  95 

Phe Glu Asn Ala Ala Thr Val Met Arg Ala Gly Ala Asn Met Val Lys
            100                 105                 110 

Ile Glu Gly Ile Glu Trp Leu Val Glu Thr Val Gln Met Leu Thr Glu
        115                 120                 125 

Arg Ala Val Pro Val Cys Gly His Leu Gly Leu Thr Pro Gln Ser Val
    130                 135                 140 

Asn Ile Phe Gly Gly Tyr Lys Val Gln Gly Arg Gly Asp Glu Ala Gly
145                 150                 155                 160 

Asp Gln Leu Leu Ser Asp Ala Leu Ala Leu Glu Ala Ala Gly Ala Gln
                165                 170                 175 

Leu Leu Val Leu Glu Cys Val Pro Val Glu Leu Ala Lys Arg Ile Thr
            180                 185                 190 

Glu Ala Leu Ala Ile Pro Val Ile Gly Ile Gly Ala Gly Asn Val Thr
        195                 200                 205 

Asp Gly Gln Ile Leu Val Met His Asp Ala Phe Gly Ile Thr Gly Gly
    210                 215                 220 

His Ile Pro Lys Phe Ala Lys Asn Phe Leu Ala Glu Thr Gly Asp Ile
225                 230                 235                 240 

Arg Ala Ala Val Arg Gln Tyr Met Ala Glu Val Glu Ser Gly Val Tyr
                245                 250                 255 

Pro Gly Glu Glu His Ser Phe His
            260                


<210>    69
<211>    264
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    synthetic_PanB with G116L mutation


<400>    69
Met Lys Pro Thr Thr Ile Ser Leu Leu Gln Lys Tyr Lys Gln Glu Lys
  1               5                  10                  15 

Lys Arg Phe Ala Thr Ile Thr Ala Tyr Asp Tyr Ser Phe Ala Lys Leu
             20                  25                  30 

Phe Ala Asp Glu Gly Leu Asn Val Met Leu Val Gly Asp Ser Leu Gly
         35                  40                  45 

Met Thr Val Gln Gly His Asp Ser Thr Leu Pro Val Thr Val Ala Asp
     50                  55                  60 

Ile Ala Tyr His Thr Ala Ala Val Arg Arg Gly Ala Pro Asn Cys Leu
 65                  70                  75                  80 

Leu Leu Ala Asp Leu Pro Phe Met Ala Tyr Ala Thr Pro Glu Gln Ala
                 85                  90                  95 

Phe Glu Asn Ala Ala Thr Val Met Arg Ala Gly Ala Asn Met Val Lys
            100                 105                 110 

Ile Glu Gly Leu Glu Trp Leu Val Glu Thr Val Gln Met Leu Thr Glu
        115                 120                 125 

Arg Ala Val Pro Val Cys Gly His Leu Gly Leu Thr Pro Gln Ser Val
    130                 135                 140 

Asn Ile Phe Gly Gly Tyr Lys Val Gln Gly Arg Gly Asp Glu Ala Gly
145                 150                 155                 160 

Asp Gln Leu Leu Ser Asp Ala Leu Ala Leu Glu Ala Ala Gly Ala Gln
                165                 170                 175 

Leu Leu Val Leu Glu Cys Val Pro Val Glu Leu Ala Lys Arg Ile Thr
            180                 185                 190 

Glu Ala Leu Ala Ile Pro Val Ile Gly Ile Gly Ala Gly Asn Val Thr
        195                 200                 205 

Asp Gly Gln Ile Leu Val Met His Asp Ala Phe Gly Ile Thr Gly Gly
    210                 215                 220 

His Ile Pro Lys Phe Ala Lys Asn Phe Leu Ala Glu Thr Gly Asp Ile
225                 230                 235                 240 

Arg Ala Ala Val Arg Gln Tyr Met Ala Glu Val Glu Ser Gly Val Tyr
                245                 250                 255 

Pro Gly Glu Glu His Ser Phe His
            260                


<210>    70
<211>    264
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    synthetic_PanB with G116D mutation


<400>    70
Met Lys Pro Thr Thr Ile Ser Leu Leu Gln Lys Tyr Lys Gln Glu Lys
  1               5                  10                  15 

Lys Arg Phe Ala Thr Ile Thr Ala Tyr Asp Tyr Ser Phe Ala Lys Leu
             20                  25                  30 

Phe Ala Asp Glu Gly Leu Asn Val Met Leu Val Gly Asp Ser Leu Gly
         35                  40                  45 

Met Thr Val Gln Gly His Asp Ser Thr Leu Pro Val Thr Val Ala Asp
     50                  55                  60 

Ile Ala Tyr His Thr Ala Ala Val Arg Arg Gly Ala Pro Asn Cys Leu
 65                  70                  75                  80 

Leu Leu Ala Asp Leu Pro Phe Met Ala Tyr Ala Thr Pro Glu Gln Ala
                 85                  90                  95 

Phe Glu Asn Ala Ala Thr Val Met Arg Ala Gly Ala Asn Met Val Lys
            100                 105                 110 

Ile Glu Gly Asp Glu Trp Leu Val Glu Thr Val Gln Met Leu Thr Glu
        115                 120                 125 

Arg Ala Val Pro Val Cys Gly His Leu Gly Leu Thr Pro Gln Ser Val
    130                 135                 140 

Asn Ile Phe Gly Gly Tyr Lys Val Gln Gly Arg Gly Asp Glu Ala Gly
145                 150                 155                 160 

Asp Gln Leu Leu Ser Asp Ala Leu Ala Leu Glu Ala Ala Gly Ala Gln
                165                 170                 175 

Leu Leu Val Leu Glu Cys Val Pro Val Glu Leu Ala Lys Arg Ile Thr
            180                 185                 190 

Glu Ala Leu Ala Ile Pro Val Ile Gly Ile Gly Ala Gly Asn Val Thr
        195                 200                 205 

Asp Gly Gln Ile Leu Val Met His Asp Ala Phe Gly Ile Thr Gly Gly
    210                 215                 220 

His Ile Pro Lys Phe Ala Lys Asn Phe Leu Ala Glu Thr Gly Asp Ile
225                 230                 235                 240 

Arg Ala Ala Val Arg Gln Tyr Met Ala Glu Val Glu Ser Gly Val Tyr
                245                 250                 255 

Pro Gly Glu Glu His Ser Phe His
            260                


<210>    71
<211>    264
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    synthetic_PanB with G116C mutation


<400>    71
Met Lys Pro Thr Thr Ile Ser Leu Leu Gln Lys Tyr Lys Gln Glu Lys
  1               5                  10                  15 

Lys Arg Phe Ala Thr Ile Thr Ala Tyr Asp Tyr Ser Phe Ala Lys Leu
             20                  25                  30 

Phe Ala Asp Glu Gly Leu Asn Val Met Leu Val Gly Asp Ser Leu Gly
         35                  40                  45 

Met Thr Val Gln Gly His Asp Ser Thr Leu Pro Val Thr Val Ala Asp
     50                  55                  60 

Ile Ala Tyr His Thr Ala Ala Val Arg Arg Gly Ala Pro Asn Cys Leu
 65                  70                  75                  80 

Leu Leu Ala Asp Leu Pro Phe Met Ala Tyr Ala Thr Pro Glu Gln Ala
                 85                  90                  95 

Phe Glu Asn Ala Ala Thr Val Met Arg Ala Gly Ala Asn Met Val Lys
            100                 105                 110 

Ile Glu Gly Cys Glu Trp Leu Val Glu Thr Val Gln Met Leu Thr Glu
        115                 120                 125 

Arg Ala Val Pro Val Cys Gly His Leu Gly Leu Thr Pro Gln Ser Val
    130                 135                 140 

Asn Ile Phe Gly Gly Tyr Lys Val Gln Gly Arg Gly Asp Glu Ala Gly
145                 150                 155                 160 

Asp Gln Leu Leu Ser Asp Ala Leu Ala Leu Glu Ala Ala Gly Ala Gln
                165                 170                 175 

Leu Leu Val Leu Glu Cys Val Pro Val Glu Leu Ala Lys Arg Ile Thr
            180                 185                 190 

Glu Ala Leu Ala Ile Pro Val Ile Gly Ile Gly Ala Gly Asn Val Thr
        195                 200                 205 

Asp Gly Gln Ile Leu Val Met His Asp Ala Phe Gly Ile Thr Gly Gly
    210                 215                 220 

His Ile Pro Lys Phe Ala Lys Asn Phe Leu Ala Glu Thr Gly Asp Ile
225                 230                 235                 240 

Arg Ala Ala Val Arg Gln Tyr Met Ala Glu Val Glu Ser Gly Val Tyr
                245                 250                 255 

Pro Gly Glu Glu His Ser Phe His
            260                


<210>    72
<211>    264
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    synthetic_PanB with G116Q mutation


<400>    72
Met Lys Pro Thr Thr Ile Ser Leu Leu Gln Lys Tyr Lys Gln Glu Lys
  1               5                  10                  15 

Lys Arg Phe Ala Thr Ile Thr Ala Tyr Asp Tyr Ser Phe Ala Lys Leu
             20                  25                  30 

Phe Ala Asp Glu Gly Leu Asn Val Met Leu Val Gly Asp Ser Leu Gly
         35                  40                  45 

Met Thr Val Gln Gly His Asp Ser Thr Leu Pro Val Thr Val Ala Asp
     50                  55                  60 

Ile Ala Tyr His Thr Ala Ala Val Arg Arg Gly Ala Pro Asn Cys Leu
 65                  70                  75                  80 

Leu Leu Ala Asp Leu Pro Phe Met Ala Tyr Ala Thr Pro Glu Gln Ala
                 85                  90                  95 

Phe Glu Asn Ala Ala Thr Val Met Arg Ala Gly Ala Asn Met Val Lys
            100                 105                 110 

Ile Glu Gly Gln Glu Trp Leu Val Glu Thr Val Gln Met Leu Thr Glu
        115                 120                 125 

Arg Ala Val Pro Val Cys Gly His Leu Gly Leu Thr Pro Gln Ser Val
    130                 135                 140 

Asn Ile Phe Gly Gly Tyr Lys Val Gln Gly Arg Gly Asp Glu Ala Gly
145                 150                 155                 160 

Asp Gln Leu Leu Ser Asp Ala Leu Ala Leu Glu Ala Ala Gly Ala Gln
                165                 170                 175 

Leu Leu Val Leu Glu Cys Val Pro Val Glu Leu Ala Lys Arg Ile Thr
            180                 185                 190 

Glu Ala Leu Ala Ile Pro Val Ile Gly Ile Gly Ala Gly Asn Val Thr
        195                 200                 205 

Asp Gly Gln Ile Leu Val Met His Asp Ala Phe Gly Ile Thr Gly Gly
    210                 215                 220 

His Ile Pro Lys Phe Ala Lys Asn Phe Leu Ala Glu Thr Gly Asp Ile
225                 230                 235                 240 

Arg Ala Ala Val Arg Gln Tyr Met Ala Glu Val Glu Ser Gly Val Tyr
                245                 250                 255 

Pro Gly Glu Glu His Ser Phe His
            260                


<210>    73
<211>    264
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    synthetic_PanB with G116M mutation


<400>    73
Met Lys Pro Thr Thr Ile Ser Leu Leu Gln Lys Tyr Lys Gln Glu Lys
  1               5                  10                  15 

Lys Arg Phe Ala Thr Ile Thr Ala Tyr Asp Tyr Ser Phe Ala Lys Leu
             20                  25                  30 

Phe Ala Asp Glu Gly Leu Asn Val Met Leu Val Gly Asp Ser Leu Gly
         35                  40                  45 

Met Thr Val Gln Gly His Asp Ser Thr Leu Pro Val Thr Val Ala Asp
     50                  55                  60 

Ile Ala Tyr His Thr Ala Ala Val Arg Arg Gly Ala Pro Asn Cys Leu
 65                  70                  75                  80 

Leu Leu Ala Asp Leu Pro Phe Met Ala Tyr Ala Thr Pro Glu Gln Ala
                 85                  90                  95 

Phe Glu Asn Ala Ala Thr Val Met Arg Ala Gly Ala Asn Met Val Lys
            100                 105                 110 

Ile Glu Gly Met Glu Trp Leu Val Glu Thr Val Gln Met Leu Thr Glu
        115                 120                 125 

Arg Ala Val Pro Val Cys Gly His Leu Gly Leu Thr Pro Gln Ser Val
    130                 135                 140 

Asn Ile Phe Gly Gly Tyr Lys Val Gln Gly Arg Gly Asp Glu Ala Gly
145                 150                 155                 160 

Asp Gln Leu Leu Ser Asp Ala Leu Ala Leu Glu Ala Ala Gly Ala Gln
                165                 170                 175 

Leu Leu Val Leu Glu Cys Val Pro Val Glu Leu Ala Lys Arg Ile Thr
            180                 185                 190 

Glu Ala Leu Ala Ile Pro Val Ile Gly Ile Gly Ala Gly Asn Val Thr
        195                 200                 205 

Asp Gly Gln Ile Leu Val Met His Asp Ala Phe Gly Ile Thr Gly Gly
    210                 215                 220 

His Ile Pro Lys Phe Ala Lys Asn Phe Leu Ala Glu Thr Gly Asp Ile
225                 230                 235                 240 

Arg Ala Ala Val Arg Gln Tyr Met Ala Glu Val Glu Ser Gly Val Tyr
                245                 250                 255 

Pro Gly Glu Glu His Ser Phe His
            260                


<210>    74
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    74
agatcgaaag gtgcacaaag atgaaaccga ccaccatctc                               40


<210>    75
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    75
aggggcgcgg cgatgaacgc ggcgatcaac tgctcagcga                               40


<210>    76
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    76
ctgagcagtt gatcgccgcg ttcatcgccg cgcccctgaa                               40


<210>    77
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    77
cgctctagaa ctagtggatc ttaatggaaa ctgtgttctt                               40


<210>    78
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    78
aggggcgcgg cgatgaatcc ggcgatcaac tgctcagcga                               40


<210>    79
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    79
ctgagcagtt gatcgccgga ttcatcgccg cgcccctgaa                               40


<210>    80
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    80
aggggcgcgg cgatgaatac ggcgatcaac tgctcagcga                               40


<210>    81
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    81
ctgagcagtt gatcgccgta ttcatcgccg cgcccctgaa                               40


<210>    82
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    82
aggggcgcgg cgatgaatgc ggcgatcaac tgctcagcga                               40


<210>    83
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    83
ctgagcagtt gatcgccgca ttcatcgccg cgcccctgaa                               40


<210>    84
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    84
aggggcgcgg cgatgaacca ggcgatcaac tgctcagcga                               40


<210>    85
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    85
ctgagcagtt gatcgcctgg ttcatcgccg cgcccctgaa                               40


<210>    86
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    86
aggggcgcgg cgatgaacac ggcgatcaac tgctcagcga                               40


<210>    87
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    87
ctgagcagtt gatcgccgtg ttcatcgccg cgcccctgaa                               40


<210>    88
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    88
aggggcgcgg cgatgaactg ggcgatcaac tgctcagcga                               40


<210>    89
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    89
ctgagcagtt gatcgcccag ttcatcgccg cgcccctgaa                               40


<210>    90
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    90
aggggcgcgg cgatgaaatc ggcgatcaac tgctcagcga                               40


<210>    91
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    91
ctgagcagtt gatcgccgat ttcatcgccg cgcccctgaa                               40


<210>    92
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    92
aggggcgcgg cgatgaaacc ggcgatcaac tgctcagcga                               40


<210>    93
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    93
ctgagcagtt gatcgccggt ttcatcgccg cgcccctgaa                               40


<210>    94
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    94
aggggcgcgg cgatgaaaag ggcgatcaac tgctcagcga                               40


<210>    95
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    95
ctgagcagtt gatcgccctt ttcatcgccg cgcccctgaa                               40


<210>    96
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    96
aggggcgcgg cgatgaagtg ggcgatcaac tgctcagcga                               40


<210>    97
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    97
ctgagcagtt gatcgcccac ttcatcgccg cgcccctgaa                               40


<210>    98
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    98
aggggcgcgg cgatgaaatg ggcgatcaac tgctcagcga                               40


<210>    99
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    99
ctgagcagtt gatcgcccat ttcatcgccg cgcccctgaa                               40


<210>    100
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    100
aggggcgcgg cgatgaagat ggcgatcaac tgctcagcga                               40


<210>    101
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    101
ctgagcagtt gatcgccatc ttcatcgccg cgcccctgaa                               40


<210>    102
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    102
aggggcgcgg cgatgaacag ggcgatcaac tgctcagcga                               40


<210>    103
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    103
ctgagcagtt gatcgccctg ttcatcgccg cgcccctgaa                               40


<210>    104
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    104
aggggcgcgg cgatgaaaac ggcgatcaac tgctcagcga                               40


<210>    105
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    105
ctgagcagtt gatcgccgtt ttcatcgccg cgcccctgaa                               40


<210>    106
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    106
aggggcgcgg cgatgaacag ggcgatcaac tgctcagcga                               40


<210>    107
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    107
ctgagcagtt gatcgccctg ttcatcgccg cgcccctgaa                               40


<210>    108
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    108
cttgatatcg aattcctgca ttcagggtag ttgactaaag                               40


<210>    109
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    109
gagatggtgg tcggtttcat ctttgtgcac ctttcgatct                               40


<210>    110
<211>    264
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PanB with A159L mutation


<400>    110
Met Lys Pro Thr Thr Ile Ser Leu Leu Gln Lys Tyr Lys Gln Glu Lys
  1               5                  10                  15 

Lys Arg Phe Ala Thr Ile Thr Ala Tyr Asp Tyr Ser Phe Ala Lys Leu
             20                  25                  30 

Phe Ala Asp Glu Gly Leu Asn Val Met Leu Val Gly Asp Ser Leu Gly
         35                  40                  45 

Met Thr Val Gln Gly His Asp Ser Thr Leu Pro Val Thr Val Ala Asp
     50                  55                  60 

Ile Ala Tyr His Thr Ala Ala Val Arg Arg Gly Ala Pro Asn Cys Leu
 65                  70                  75                  80 

Leu Leu Ala Asp Leu Pro Phe Met Ala Tyr Ala Thr Pro Glu Gln Ala
                 85                  90                  95 

Phe Glu Asn Ala Ala Thr Val Met Arg Ala Gly Ala Asn Met Val Lys
            100                 105                 110 

Ile Glu Gly Gly Glu Trp Leu Val Glu Thr Val Gln Met Leu Thr Glu
        115                 120                 125 

Arg Ala Val Pro Val Cys Gly His Leu Gly Leu Thr Pro Gln Ser Val
    130                 135                 140 

Asn Ile Phe Gly Gly Tyr Lys Val Gln Gly Arg Gly Asp Glu Leu Gly
145                 150                 155                 160 

Asp Gln Leu Leu Ser Asp Ala Leu Ala Leu Glu Ala Ala Gly Ala Gln
                165                 170                 175 

Leu Leu Val Leu Glu Cys Val Pro Val Glu Leu Ala Lys Arg Ile Thr
            180                 185                 190 

Glu Ala Leu Ala Ile Pro Val Ile Gly Ile Gly Ala Gly Asn Val Thr
        195                 200                 205 

Asp Gly Gln Ile Leu Val Met His Asp Ala Phe Gly Ile Thr Gly Gly
    210                 215                 220 

His Ile Pro Lys Phe Ala Lys Asn Phe Leu Ala Glu Thr Gly Asp Ile
225                 230                 235                 240 

Arg Ala Ala Val Arg Gln Tyr Met Ala Glu Val Glu Ser Gly Val Tyr
                245                 250                 255 

Pro Gly Glu Glu His Ser Phe His
            260                


<210>    111
<211>    264
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PanB with A159R mutation


<400>    111
Met Lys Pro Thr Thr Ile Ser Leu Leu Gln Lys Tyr Lys Gln Glu Lys
  1               5                  10                  15 

Lys Arg Phe Ala Thr Ile Thr Ala Tyr Asp Tyr Ser Phe Ala Lys Leu
             20                  25                  30 

Phe Ala Asp Glu Gly Leu Asn Val Met Leu Val Gly Asp Ser Leu Gly
         35                  40                  45 

Met Thr Val Gln Gly His Asp Ser Thr Leu Pro Val Thr Val Ala Asp
     50                  55                  60 

Ile Ala Tyr His Thr Ala Ala Val Arg Arg Gly Ala Pro Asn Cys Leu
 65                  70                  75                  80 

Leu Leu Ala Asp Leu Pro Phe Met Ala Tyr Ala Thr Pro Glu Gln Ala
                 85                  90                  95 

Phe Glu Asn Ala Ala Thr Val Met Arg Ala Gly Ala Asn Met Val Lys
            100                 105                 110 

Ile Glu Gly Gly Glu Trp Leu Val Glu Thr Val Gln Met Leu Thr Glu
        115                 120                 125 

Arg Ala Val Pro Val Cys Gly His Leu Gly Leu Thr Pro Gln Ser Val
    130                 135                 140 

Asn Ile Phe Gly Gly Tyr Lys Val Gln Gly Arg Gly Asp Glu Arg Gly
145                 150                 155                 160 

Asp Gln Leu Leu Ser Asp Ala Leu Ala Leu Glu Ala Ala Gly Ala Gln
                165                 170                 175 

Leu Leu Val Leu Glu Cys Val Pro Val Glu Leu Ala Lys Arg Ile Thr
            180                 185                 190 

Glu Ala Leu Ala Ile Pro Val Ile Gly Ile Gly Ala Gly Asn Val Thr
        195                 200                 205 

Asp Gly Gln Ile Leu Val Met His Asp Ala Phe Gly Ile Thr Gly Gly
    210                 215                 220 

His Ile Pro Lys Phe Ala Lys Asn Phe Leu Ala Glu Thr Gly Asp Ile
225                 230                 235                 240 

Arg Ala Ala Val Arg Gln Tyr Met Ala Glu Val Glu Ser Gly Val Tyr
                245                 250                 255 

Pro Gly Glu Glu His Ser Phe His
            260                


<210>    112
<211>    264
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PanB with A159S mutation


<400>    112
Met Lys Pro Thr Thr Ile Ser Leu Leu Gln Lys Tyr Lys Gln Glu Lys
  1               5                  10                  15 

Lys Arg Phe Ala Thr Ile Thr Ala Tyr Asp Tyr Ser Phe Ala Lys Leu
             20                  25                  30 

Phe Ala Asp Glu Gly Leu Asn Val Met Leu Val Gly Asp Ser Leu Gly
         35                  40                  45 

Met Thr Val Gln Gly His Asp Ser Thr Leu Pro Val Thr Val Ala Asp
     50                  55                  60 

Ile Ala Tyr His Thr Ala Ala Val Arg Arg Gly Ala Pro Asn Cys Leu
 65                  70                  75                  80 

Leu Leu Ala Asp Leu Pro Phe Met Ala Tyr Ala Thr Pro Glu Gln Ala
                 85                  90                  95 

Phe Glu Asn Ala Ala Thr Val Met Arg Ala Gly Ala Asn Met Val Lys
            100                 105                 110 

Ile Glu Gly Gly Glu Trp Leu Val Glu Thr Val Gln Met Leu Thr Glu
        115                 120                 125 

Arg Ala Val Pro Val Cys Gly His Leu Gly Leu Thr Pro Gln Ser Val
    130                 135                 140 

Asn Ile Phe Gly Gly Tyr Lys Val Gln Gly Arg Gly Asp Glu Ser Gly
145                 150                 155                 160 

Asp Gln Leu Leu Ser Asp Ala Leu Ala Leu Glu Ala Ala Gly Ala Gln
                165                 170                 175 

Leu Leu Val Leu Glu Cys Val Pro Val Glu Leu Ala Lys Arg Ile Thr
            180                 185                 190 

Glu Ala Leu Ala Ile Pro Val Ile Gly Ile Gly Ala Gly Asn Val Thr
        195                 200                 205 

Asp Gly Gln Ile Leu Val Met His Asp Ala Phe Gly Ile Thr Gly Gly
    210                 215                 220 

His Ile Pro Lys Phe Ala Lys Asn Phe Leu Ala Glu Thr Gly Asp Ile
225                 230                 235                 240 

Arg Ala Ala Val Arg Gln Tyr Met Ala Glu Val Glu Ser Gly Val Tyr
                245                 250                 255 

Pro Gly Glu Glu His Ser Phe His
            260                


<210>    113
<211>    264
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PanB with A159Y mutation


<400>    113
Met Lys Pro Thr Thr Ile Ser Leu Leu Gln Lys Tyr Lys Gln Glu Lys
  1               5                  10                  15 

Lys Arg Phe Ala Thr Ile Thr Ala Tyr Asp Tyr Ser Phe Ala Lys Leu
             20                  25                  30 

Phe Ala Asp Glu Gly Leu Asn Val Met Leu Val Gly Asp Ser Leu Gly
         35                  40                  45 

Met Thr Val Gln Gly His Asp Ser Thr Leu Pro Val Thr Val Ala Asp
     50                  55                  60 

Ile Ala Tyr His Thr Ala Ala Val Arg Arg Gly Ala Pro Asn Cys Leu
 65                  70                  75                  80 

Leu Leu Ala Asp Leu Pro Phe Met Ala Tyr Ala Thr Pro Glu Gln Ala
                 85                  90                  95 

Phe Glu Asn Ala Ala Thr Val Met Arg Ala Gly Ala Asn Met Val Lys
            100                 105                 110 

Ile Glu Gly Gly Glu Trp Leu Val Glu Thr Val Gln Met Leu Thr Glu
        115                 120                 125 

Arg Ala Val Pro Val Cys Gly His Leu Gly Leu Thr Pro Gln Ser Val
    130                 135                 140 

Asn Ile Phe Gly Gly Tyr Lys Val Gln Gly Arg Gly Asp Glu Tyr Gly
145                 150                 155                 160 

Asp Gln Leu Leu Ser Asp Ala Leu Ala Leu Glu Ala Ala Gly Ala Gln
                165                 170                 175 

Leu Leu Val Leu Glu Cys Val Pro Val Glu Leu Ala Lys Arg Ile Thr
            180                 185                 190 

Glu Ala Leu Ala Ile Pro Val Ile Gly Ile Gly Ala Gly Asn Val Thr
        195                 200                 205 

Asp Gly Gln Ile Leu Val Met His Asp Ala Phe Gly Ile Thr Gly Gly
    210                 215                 220 

His Ile Pro Lys Phe Ala Lys Asn Phe Leu Ala Glu Thr Gly Asp Ile
225                 230                 235                 240 

Arg Ala Ala Val Arg Gln Tyr Met Ala Glu Val Glu Ser Gly Val Tyr
                245                 250                 255 

Pro Gly Glu Glu His Ser Phe His
            260                


<210>    114
<211>    264
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PanB with A159C mutation


<400>    114
Met Lys Pro Thr Thr Ile Ser Leu Leu Gln Lys Tyr Lys Gln Glu Lys
  1               5                  10                  15 

Lys Arg Phe Ala Thr Ile Thr Ala Tyr Asp Tyr Ser Phe Ala Lys Leu
             20                  25                  30 

Phe Ala Asp Glu Gly Leu Asn Val Met Leu Val Gly Asp Ser Leu Gly
         35                  40                  45 

Met Thr Val Gln Gly His Asp Ser Thr Leu Pro Val Thr Val Ala Asp
     50                  55                  60 

Ile Ala Tyr His Thr Ala Ala Val Arg Arg Gly Ala Pro Asn Cys Leu
 65                  70                  75                  80 

Leu Leu Ala Asp Leu Pro Phe Met Ala Tyr Ala Thr Pro Glu Gln Ala
                 85                  90                  95 

Phe Glu Asn Ala Ala Thr Val Met Arg Ala Gly Ala Asn Met Val Lys
            100                 105                 110 

Ile Glu Gly Gly Glu Trp Leu Val Glu Thr Val Gln Met Leu Thr Glu
        115                 120                 125 

Arg Ala Val Pro Val Cys Gly His Leu Gly Leu Thr Pro Gln Ser Val
    130                 135                 140 

Asn Ile Phe Gly Gly Tyr Lys Val Gln Gly Arg Gly Asp Glu Cys Gly
145                 150                 155                 160 

Asp Gln Leu Leu Ser Asp Ala Leu Ala Leu Glu Ala Ala Gly Ala Gln
                165                 170                 175 

Leu Leu Val Leu Glu Cys Val Pro Val Glu Leu Ala Lys Arg Ile Thr
            180                 185                 190 

Glu Ala Leu Ala Ile Pro Val Ile Gly Ile Gly Ala Gly Asn Val Thr
        195                 200                 205 

Asp Gly Gln Ile Leu Val Met His Asp Ala Phe Gly Ile Thr Gly Gly
    210                 215                 220 

His Ile Pro Lys Phe Ala Lys Asn Phe Leu Ala Glu Thr Gly Asp Ile
225                 230                 235                 240 

Arg Ala Ala Val Arg Gln Tyr Met Ala Glu Val Glu Ser Gly Val Tyr
                245                 250                 255 

Pro Gly Glu Glu His Ser Phe His
            260                


<210>    115
<211>    264
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PanB with A159P mutation


<400>    115
Met Lys Pro Thr Thr Ile Ser Leu Leu Gln Lys Tyr Lys Gln Glu Lys
  1               5                  10                  15 

Lys Arg Phe Ala Thr Ile Thr Ala Tyr Asp Tyr Ser Phe Ala Lys Leu
             20                  25                  30 

Phe Ala Asp Glu Gly Leu Asn Val Met Leu Val Gly Asp Ser Leu Gly
         35                  40                  45 

Met Thr Val Gln Gly His Asp Ser Thr Leu Pro Val Thr Val Ala Asp
     50                  55                  60 

Ile Ala Tyr His Thr Ala Ala Val Arg Arg Gly Ala Pro Asn Cys Leu
 65                  70                  75                  80 

Leu Leu Ala Asp Leu Pro Phe Met Ala Tyr Ala Thr Pro Glu Gln Ala
                 85                  90                  95 

Phe Glu Asn Ala Ala Thr Val Met Arg Ala Gly Ala Asn Met Val Lys
            100                 105                 110 

Ile Glu Gly Gly Glu Trp Leu Val Glu Thr Val Gln Met Leu Thr Glu
        115                 120                 125 

Arg Ala Val Pro Val Cys Gly His Leu Gly Leu Thr Pro Gln Ser Val
    130                 135                 140 

Asn Ile Phe Gly Gly Tyr Lys Val Gln Gly Arg Gly Asp Glu Pro Gly
145                 150                 155                 160 

Asp Gln Leu Leu Ser Asp Ala Leu Ala Leu Glu Ala Ala Gly Ala Gln
                165                 170                 175 

Leu Leu Val Leu Glu Cys Val Pro Val Glu Leu Ala Lys Arg Ile Thr
            180                 185                 190 

Glu Ala Leu Ala Ile Pro Val Ile Gly Ile Gly Ala Gly Asn Val Thr
        195                 200                 205 

Asp Gly Gln Ile Leu Val Met His Asp Ala Phe Gly Ile Thr Gly Gly
    210                 215                 220 

His Ile Pro Lys Phe Ala Lys Asn Phe Leu Ala Glu Thr Gly Asp Ile
225                 230                 235                 240 

Arg Ala Ala Val Arg Gln Tyr Met Ala Glu Val Glu Ser Gly Val Tyr
                245                 250                 255 

Pro Gly Glu Glu His Ser Phe His
            260                


<210>    116
<211>    264
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PanB with A159H mutation


<400>    116
Met Lys Pro Thr Thr Ile Ser Leu Leu Gln Lys Tyr Lys Gln Glu Lys
  1               5                  10                  15 

Lys Arg Phe Ala Thr Ile Thr Ala Tyr Asp Tyr Ser Phe Ala Lys Leu
             20                  25                  30 

Phe Ala Asp Glu Gly Leu Asn Val Met Leu Val Gly Asp Ser Leu Gly
         35                  40                  45 

Met Thr Val Gln Gly His Asp Ser Thr Leu Pro Val Thr Val Ala Asp
     50                  55                  60 

Ile Ala Tyr His Thr Ala Ala Val Arg Arg Gly Ala Pro Asn Cys Leu
 65                  70                  75                  80 

Leu Leu Ala Asp Leu Pro Phe Met Ala Tyr Ala Thr Pro Glu Gln Ala
                 85                  90                  95 

Phe Glu Asn Ala Ala Thr Val Met Arg Ala Gly Ala Asn Met Val Lys
            100                 105                 110 

Ile Glu Gly Gly Glu Trp Leu Val Glu Thr Val Gln Met Leu Thr Glu
        115                 120                 125 

Arg Ala Val Pro Val Cys Gly His Leu Gly Leu Thr Pro Gln Ser Val
    130                 135                 140 

Asn Ile Phe Gly Gly Tyr Lys Val Gln Gly Arg Gly Asp Glu His Gly
145                 150                 155                 160 

Asp Gln Leu Leu Ser Asp Ala Leu Ala Leu Glu Ala Ala Gly Ala Gln
                165                 170                 175 

Leu Leu Val Leu Glu Cys Val Pro Val Glu Leu Ala Lys Arg Ile Thr
            180                 185                 190 

Glu Ala Leu Ala Ile Pro Val Ile Gly Ile Gly Ala Gly Asn Val Thr
        195                 200                 205 

Asp Gly Gln Ile Leu Val Met His Asp Ala Phe Gly Ile Thr Gly Gly
    210                 215                 220 

His Ile Pro Lys Phe Ala Lys Asn Phe Leu Ala Glu Thr Gly Asp Ile
225                 230                 235                 240 

Arg Ala Ala Val Arg Gln Tyr Met Ala Glu Val Glu Ser Gly Val Tyr
                245                 250                 255 

Pro Gly Glu Glu His Ser Phe His
            260                


<210>    117
<211>    264
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PanB with A159I mutation


<400>    117
Met Lys Pro Thr Thr Ile Ser Leu Leu Gln Lys Tyr Lys Gln Glu Lys
  1               5                  10                  15 

Lys Arg Phe Ala Thr Ile Thr Ala Tyr Asp Tyr Ser Phe Ala Lys Leu
             20                  25                  30 

Phe Ala Asp Glu Gly Leu Asn Val Met Leu Val Gly Asp Ser Leu Gly
         35                  40                  45 

Met Thr Val Gln Gly His Asp Ser Thr Leu Pro Val Thr Val Ala Asp
     50                  55                  60 

Ile Ala Tyr His Thr Ala Ala Val Arg Arg Gly Ala Pro Asn Cys Leu
 65                  70                  75                  80 

Leu Leu Ala Asp Leu Pro Phe Met Ala Tyr Ala Thr Pro Glu Gln Ala
                 85                  90                  95 

Phe Glu Asn Ala Ala Thr Val Met Arg Ala Gly Ala Asn Met Val Lys
            100                 105                 110 

Ile Glu Gly Gly Glu Trp Leu Val Glu Thr Val Gln Met Leu Thr Glu
        115                 120                 125 

Arg Ala Val Pro Val Cys Gly His Leu Gly Leu Thr Pro Gln Ser Val
    130                 135                 140 

Asn Ile Phe Gly Gly Tyr Lys Val Gln Gly Arg Gly Asp Glu Ile Gly
145                 150                 155                 160 

Asp Gln Leu Leu Ser Asp Ala Leu Ala Leu Glu Ala Ala Gly Ala Gln
                165                 170                 175 

Leu Leu Val Leu Glu Cys Val Pro Val Glu Leu Ala Lys Arg Ile Thr
            180                 185                 190 

Glu Ala Leu Ala Ile Pro Val Ile Gly Ile Gly Ala Gly Asn Val Thr
        195                 200                 205 

Asp Gly Gln Ile Leu Val Met His Asp Ala Phe Gly Ile Thr Gly Gly
    210                 215                 220 

His Ile Pro Lys Phe Ala Lys Asn Phe Leu Ala Glu Thr Gly Asp Ile
225                 230                 235                 240 

Arg Ala Ala Val Arg Gln Tyr Met Ala Glu Val Glu Ser Gly Val Tyr
                245                 250                 255 

Pro Gly Glu Glu His Ser Phe His
            260                


<210>    118
<211>    264
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PanB with A159T mutation


<400>    118
Met Lys Pro Thr Thr Ile Ser Leu Leu Gln Lys Tyr Lys Gln Glu Lys
  1               5                  10                  15 

Lys Arg Phe Ala Thr Ile Thr Ala Tyr Asp Tyr Ser Phe Ala Lys Leu
             20                  25                  30 

Phe Ala Asp Glu Gly Leu Asn Val Met Leu Val Gly Asp Ser Leu Gly
         35                  40                  45 

Met Thr Val Gln Gly His Asp Ser Thr Leu Pro Val Thr Val Ala Asp
     50                  55                  60 

Ile Ala Tyr His Thr Ala Ala Val Arg Arg Gly Ala Pro Asn Cys Leu
 65                  70                  75                  80 

Leu Leu Ala Asp Leu Pro Phe Met Ala Tyr Ala Thr Pro Glu Gln Ala
                 85                  90                  95 

Phe Glu Asn Ala Ala Thr Val Met Arg Ala Gly Ala Asn Met Val Lys
            100                 105                 110 

Ile Glu Gly Gly Glu Trp Leu Val Glu Thr Val Gln Met Leu Thr Glu
        115                 120                 125 

Arg Ala Val Pro Val Cys Gly His Leu Gly Leu Thr Pro Gln Ser Val
    130                 135                 140 

Asn Ile Phe Gly Gly Tyr Lys Val Gln Gly Arg Gly Asp Glu Thr Gly
145                 150                 155                 160 

Asp Gln Leu Leu Ser Asp Ala Leu Ala Leu Glu Ala Ala Gly Ala Gln
                165                 170                 175 

Leu Leu Val Leu Glu Cys Val Pro Val Glu Leu Ala Lys Arg Ile Thr
            180                 185                 190 

Glu Ala Leu Ala Ile Pro Val Ile Gly Ile Gly Ala Gly Asn Val Thr
        195                 200                 205 

Asp Gly Gln Ile Leu Val Met His Asp Ala Phe Gly Ile Thr Gly Gly
    210                 215                 220 

His Ile Pro Lys Phe Ala Lys Asn Phe Leu Ala Glu Thr Gly Asp Ile
225                 230                 235                 240 

Arg Ala Ala Val Arg Gln Tyr Met Ala Glu Val Glu Ser Gly Val Tyr
                245                 250                 255 

Pro Gly Glu Glu His Ser Phe His
            260                


<210>    119
<211>    264
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PanB with A159K mutation


<400>    119
Met Lys Pro Thr Thr Ile Ser Leu Leu Gln Lys Tyr Lys Gln Glu Lys
  1               5                  10                  15 

Lys Arg Phe Ala Thr Ile Thr Ala Tyr Asp Tyr Ser Phe Ala Lys Leu
             20                  25                  30 

Phe Ala Asp Glu Gly Leu Asn Val Met Leu Val Gly Asp Ser Leu Gly
         35                  40                  45 

Met Thr Val Gln Gly His Asp Ser Thr Leu Pro Val Thr Val Ala Asp
     50                  55                  60 

Ile Ala Tyr His Thr Ala Ala Val Arg Arg Gly Ala Pro Asn Cys Leu
 65                  70                  75                  80 

Leu Leu Ala Asp Leu Pro Phe Met Ala Tyr Ala Thr Pro Glu Gln Ala
                 85                  90                  95 

Phe Glu Asn Ala Ala Thr Val Met Arg Ala Gly Ala Asn Met Val Lys
            100                 105                 110 

Ile Glu Gly Gly Glu Trp Leu Val Glu Thr Val Gln Met Leu Thr Glu
        115                 120                 125 

Arg Ala Val Pro Val Cys Gly His Leu Gly Leu Thr Pro Gln Ser Val
    130                 135                 140 

Asn Ile Phe Gly Gly Tyr Lys Val Gln Gly Arg Gly Asp Glu Lys Gly
145                 150                 155                 160 

Asp Gln Leu Leu Ser Asp Ala Leu Ala Leu Glu Ala Ala Gly Ala Gln
                165                 170                 175 

Leu Leu Val Leu Glu Cys Val Pro Val Glu Leu Ala Lys Arg Ile Thr
            180                 185                 190 

Glu Ala Leu Ala Ile Pro Val Ile Gly Ile Gly Ala Gly Asn Val Thr
        195                 200                 205 

Asp Gly Gln Ile Leu Val Met His Asp Ala Phe Gly Ile Thr Gly Gly
    210                 215                 220 

His Ile Pro Lys Phe Ala Lys Asn Phe Leu Ala Glu Thr Gly Asp Ile
225                 230                 235                 240 

Arg Ala Ala Val Arg Gln Tyr Met Ala Glu Val Glu Ser Gly Val Tyr
                245                 250                 255 

Pro Gly Glu Glu His Ser Phe His
            260                


<210>    120
<211>    264
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PanB with A159V mutation


<400>    120
Met Lys Pro Thr Thr Ile Ser Leu Leu Gln Lys Tyr Lys Gln Glu Lys
  1               5                  10                  15 

Lys Arg Phe Ala Thr Ile Thr Ala Tyr Asp Tyr Ser Phe Ala Lys Leu
             20                  25                  30 

Phe Ala Asp Glu Gly Leu Asn Val Met Leu Val Gly Asp Ser Leu Gly
         35                  40                  45 

Met Thr Val Gln Gly His Asp Ser Thr Leu Pro Val Thr Val Ala Asp
     50                  55                  60 

Ile Ala Tyr His Thr Ala Ala Val Arg Arg Gly Ala Pro Asn Cys Leu
 65                  70                  75                  80 

Leu Leu Ala Asp Leu Pro Phe Met Ala Tyr Ala Thr Pro Glu Gln Ala
                 85                  90                  95 

Phe Glu Asn Ala Ala Thr Val Met Arg Ala Gly Ala Asn Met Val Lys
            100                 105                 110 

Ile Glu Gly Gly Glu Trp Leu Val Glu Thr Val Gln Met Leu Thr Glu
        115                 120                 125 

Arg Ala Val Pro Val Cys Gly His Leu Gly Leu Thr Pro Gln Ser Val
    130                 135                 140 

Asn Ile Phe Gly Gly Tyr Lys Val Gln Gly Arg Gly Asp Glu Val Gly
145                 150                 155                 160 

Asp Gln Leu Leu Ser Asp Ala Leu Ala Leu Glu Ala Ala Gly Ala Gln
                165                 170                 175 

Leu Leu Val Leu Glu Cys Val Pro Val Glu Leu Ala Lys Arg Ile Thr
            180                 185                 190 

Glu Ala Leu Ala Ile Pro Val Ile Gly Ile Gly Ala Gly Asn Val Thr
        195                 200                 205 

Asp Gly Gln Ile Leu Val Met His Asp Ala Phe Gly Ile Thr Gly Gly
    210                 215                 220 

His Ile Pro Lys Phe Ala Lys Asn Phe Leu Ala Glu Thr Gly Asp Ile
225                 230                 235                 240 

Arg Ala Ala Val Arg Gln Tyr Met Ala Glu Val Glu Ser Gly Val Tyr
                245                 250                 255 

Pro Gly Glu Glu His Ser Phe His
            260                


<210>    121
<211>    264
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PanB with A159M mutation


<400>    121
Met Lys Pro Thr Thr Ile Ser Leu Leu Gln Lys Tyr Lys Gln Glu Lys
  1               5                  10                  15 

Lys Arg Phe Ala Thr Ile Thr Ala Tyr Asp Tyr Ser Phe Ala Lys Leu
             20                  25                  30 

Phe Ala Asp Glu Gly Leu Asn Val Met Leu Val Gly Asp Ser Leu Gly
         35                  40                  45 

Met Thr Val Gln Gly His Asp Ser Thr Leu Pro Val Thr Val Ala Asp
     50                  55                  60 

Ile Ala Tyr His Thr Ala Ala Val Arg Arg Gly Ala Pro Asn Cys Leu
 65                  70                  75                  80 

Leu Leu Ala Asp Leu Pro Phe Met Ala Tyr Ala Thr Pro Glu Gln Ala
                 85                  90                  95 

Phe Glu Asn Ala Ala Thr Val Met Arg Ala Gly Ala Asn Met Val Lys
            100                 105                 110 

Ile Glu Gly Gly Glu Trp Leu Val Glu Thr Val Gln Met Leu Thr Glu
        115                 120                 125 

Arg Ala Val Pro Val Cys Gly His Leu Gly Leu Thr Pro Gln Ser Val
    130                 135                 140 

Asn Ile Phe Gly Gly Tyr Lys Val Gln Gly Arg Gly Asp Glu Met Gly
145                 150                 155                 160 

Asp Gln Leu Leu Ser Asp Ala Leu Ala Leu Glu Ala Ala Gly Ala Gln
                165                 170                 175 

Leu Leu Val Leu Glu Cys Val Pro Val Glu Leu Ala Lys Arg Ile Thr
            180                 185                 190 

Glu Ala Leu Ala Ile Pro Val Ile Gly Ile Gly Ala Gly Asn Val Thr
        195                 200                 205 

Asp Gly Gln Ile Leu Val Met His Asp Ala Phe Gly Ile Thr Gly Gly
    210                 215                 220 

His Ile Pro Lys Phe Ala Lys Asn Phe Leu Ala Glu Thr Gly Asp Ile
225                 230                 235                 240 

Arg Ala Ala Val Arg Gln Tyr Met Ala Glu Val Glu Ser Gly Val Tyr
                245                 250                 255 

Pro Gly Glu Glu His Ser Phe His
            260                


<210>    122
<211>    264
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PanB with A159D mutation


<400>    122
Met Lys Pro Thr Thr Ile Ser Leu Leu Gln Lys Tyr Lys Gln Glu Lys
  1               5                  10                  15 

Lys Arg Phe Ala Thr Ile Thr Ala Tyr Asp Tyr Ser Phe Ala Lys Leu
             20                  25                  30 

Phe Ala Asp Glu Gly Leu Asn Val Met Leu Val Gly Asp Ser Leu Gly
         35                  40                  45 

Met Thr Val Gln Gly His Asp Ser Thr Leu Pro Val Thr Val Ala Asp
     50                  55                  60 

Ile Ala Tyr His Thr Ala Ala Val Arg Arg Gly Ala Pro Asn Cys Leu
 65                  70                  75                  80 

Leu Leu Ala Asp Leu Pro Phe Met Ala Tyr Ala Thr Pro Glu Gln Ala
                 85                  90                  95 

Phe Glu Asn Ala Ala Thr Val Met Arg Ala Gly Ala Asn Met Val Lys
            100                 105                 110 

Ile Glu Gly Gly Glu Trp Leu Val Glu Thr Val Gln Met Leu Thr Glu
        115                 120                 125 

Arg Ala Val Pro Val Cys Gly His Leu Gly Leu Thr Pro Gln Ser Val
    130                 135                 140 

Asn Ile Phe Gly Gly Tyr Lys Val Gln Gly Arg Gly Asp Glu Asp Gly
145                 150                 155                 160 

Asp Gln Leu Leu Ser Asp Ala Leu Ala Leu Glu Ala Ala Gly Ala Gln
                165                 170                 175 

Leu Leu Val Leu Glu Cys Val Pro Val Glu Leu Ala Lys Arg Ile Thr
            180                 185                 190 

Glu Ala Leu Ala Ile Pro Val Ile Gly Ile Gly Ala Gly Asn Val Thr
        195                 200                 205 

Asp Gly Gln Ile Leu Val Met His Asp Ala Phe Gly Ile Thr Gly Gly
    210                 215                 220 

His Ile Pro Lys Phe Ala Lys Asn Phe Leu Ala Glu Thr Gly Asp Ile
225                 230                 235                 240 

Arg Ala Ala Val Arg Gln Tyr Met Ala Glu Val Glu Ser Gly Val Tyr
                245                 250                 255 

Pro Gly Glu Glu His Ser Phe His
            260                


<210>    123
<211>    264
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PanB with A159E mutation


<400>    123
Met Lys Pro Thr Thr Ile Ser Leu Leu Gln Lys Tyr Lys Gln Glu Lys
  1               5                  10                  15 

Lys Arg Phe Ala Thr Ile Thr Ala Tyr Asp Tyr Ser Phe Ala Lys Leu
             20                  25                  30 

Phe Ala Asp Glu Gly Leu Asn Val Met Leu Val Gly Asp Ser Leu Gly
         35                  40                  45 

Met Thr Val Gln Gly His Asp Ser Thr Leu Pro Val Thr Val Ala Asp
     50                  55                  60 

Ile Ala Tyr His Thr Ala Ala Val Arg Arg Gly Ala Pro Asn Cys Leu
 65                  70                  75                  80 

Leu Leu Ala Asp Leu Pro Phe Met Ala Tyr Ala Thr Pro Glu Gln Ala
                 85                  90                  95 

Phe Glu Asn Ala Ala Thr Val Met Arg Ala Gly Ala Asn Met Val Lys
            100                 105                 110 

Ile Glu Gly Gly Glu Trp Leu Val Glu Thr Val Gln Met Leu Thr Glu
        115                 120                 125 

Arg Ala Val Pro Val Cys Gly His Leu Gly Leu Thr Pro Gln Ser Val
    130                 135                 140 

Asn Ile Phe Gly Gly Tyr Lys Val Gln Gly Arg Gly Asp Glu Glu Gly
145                 150                 155                 160 

Asp Gln Leu Leu Ser Asp Ala Leu Ala Leu Glu Ala Ala Gly Ala Gln
                165                 170                 175 

Leu Leu Val Leu Glu Cys Val Pro Val Glu Leu Ala Lys Arg Ile Thr
            180                 185                 190 

Glu Ala Leu Ala Ile Pro Val Ile Gly Ile Gly Ala Gly Asn Val Thr
        195                 200                 205 

Asp Gly Gln Ile Leu Val Met His Asp Ala Phe Gly Ile Thr Gly Gly
    210                 215                 220 

His Ile Pro Lys Phe Ala Lys Asn Phe Leu Ala Glu Thr Gly Asp Ile
225                 230                 235                 240 

Arg Ala Ala Val Arg Gln Tyr Met Ala Glu Val Glu Ser Gly Val Tyr
                245                 250                 255 

Pro Gly Glu Glu His Ser Phe His
            260                


<210>    124
<211>    264
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PanB with A159N mutation


<400>    124
Met Lys Pro Thr Thr Ile Ser Leu Leu Gln Lys Tyr Lys Gln Glu Lys
  1               5                  10                  15 

Lys Arg Phe Ala Thr Ile Thr Ala Tyr Asp Tyr Ser Phe Ala Lys Leu
             20                  25                  30 

Phe Ala Asp Glu Gly Leu Asn Val Met Leu Val Gly Asp Ser Leu Gly
         35                  40                  45 

Met Thr Val Gln Gly His Asp Ser Thr Leu Pro Val Thr Val Ala Asp
     50                  55                  60 

Ile Ala Tyr His Thr Ala Ala Val Arg Arg Gly Ala Pro Asn Cys Leu
 65                  70                  75                  80 

Leu Leu Ala Asp Leu Pro Phe Met Ala Tyr Ala Thr Pro Glu Gln Ala
                 85                  90                  95 

Phe Glu Asn Ala Ala Thr Val Met Arg Ala Gly Ala Asn Met Val Lys
            100                 105                 110 

Ile Glu Gly Gly Glu Trp Leu Val Glu Thr Val Gln Met Leu Thr Glu
        115                 120                 125 

Arg Ala Val Pro Val Cys Gly His Leu Gly Leu Thr Pro Gln Ser Val
    130                 135                 140 

Asn Ile Phe Gly Gly Tyr Lys Val Gln Gly Arg Gly Asp Glu Asn Gly
145                 150                 155                 160 

Asp Gln Leu Leu Ser Asp Ala Leu Ala Leu Glu Ala Ala Gly Ala Gln
                165                 170                 175 

Leu Leu Val Leu Glu Cys Val Pro Val Glu Leu Ala Lys Arg Ile Thr
            180                 185                 190 

Glu Ala Leu Ala Ile Pro Val Ile Gly Ile Gly Ala Gly Asn Val Thr
        195                 200                 205 

Asp Gly Gln Ile Leu Val Met His Asp Ala Phe Gly Ile Thr Gly Gly
    210                 215                 220 

His Ile Pro Lys Phe Ala Lys Asn Phe Leu Ala Glu Thr Gly Asp Ile
225                 230                 235                 240 

Arg Ala Ala Val Arg Gln Tyr Met Ala Glu Val Glu Ser Gly Val Tyr
                245                 250                 255 

Pro Gly Glu Glu His Ser Phe His
            260                


<210>    125
<211>    264
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PanB with A159Q mutation


<400>    125
Met Lys Pro Thr Thr Ile Ser Leu Leu Gln Lys Tyr Lys Gln Glu Lys
  1               5                  10                  15 

Lys Arg Phe Ala Thr Ile Thr Ala Tyr Asp Tyr Ser Phe Ala Lys Leu
             20                  25                  30 

Phe Ala Asp Glu Gly Leu Asn Val Met Leu Val Gly Asp Ser Leu Gly
         35                  40                  45 

Met Thr Val Gln Gly His Asp Ser Thr Leu Pro Val Thr Val Ala Asp
     50                  55                  60 

Ile Ala Tyr His Thr Ala Ala Val Arg Arg Gly Ala Pro Asn Cys Leu
 65                  70                  75                  80 

Leu Leu Ala Asp Leu Pro Phe Met Ala Tyr Ala Thr Pro Glu Gln Ala
                 85                  90                  95 

Phe Glu Asn Ala Ala Thr Val Met Arg Ala Gly Ala Asn Met Val Lys
            100                 105                 110 

Ile Glu Gly Gly Glu Trp Leu Val Glu Thr Val Gln Met Leu Thr Glu
        115                 120                 125 

Arg Ala Val Pro Val Cys Gly His Leu Gly Leu Thr Pro Gln Ser Val
    130                 135                 140 

Asn Ile Phe Gly Gly Tyr Lys Val Gln Gly Arg Gly Asp Glu Gln Gly
145                 150                 155                 160 

Asp Gln Leu Leu Ser Asp Ala Leu Ala Leu Glu Ala Ala Gly Ala Gln
                165                 170                 175 

Leu Leu Val Leu Glu Cys Val Pro Val Glu Leu Ala Lys Arg Ile Thr
            180                 185                 190 

Glu Ala Leu Ala Ile Pro Val Ile Gly Ile Gly Ala Gly Asn Val Thr
        195                 200                 205 

Asp Gly Gln Ile Leu Val Met His Asp Ala Phe Gly Ile Thr Gly Gly
    210                 215                 220 

His Ile Pro Lys Phe Ala Lys Asn Phe Leu Ala Glu Thr Gly Asp Ile
225                 230                 235                 240 

Arg Ala Ala Val Arg Gln Tyr Met Ala Glu Val Glu Ser Gly Val Tyr
                245                 250                 255 

Pro Gly Glu Glu His Ser Phe His
            260                


<210>    126
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    126
gtttctacca gccactcggc gccttcaatt ttgaccatgt                               40


<210>    127
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer


<400>    127
tggtcaaaat tgaaggcgcc gagtggctgg tagaaaccgt                               40


<210>    128
<211>    264
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    PanB with G116A and A159L mutation


<400>    128
Met Lys Pro Thr Thr Ile Ser Leu Leu Gln Lys Tyr Lys Gln Glu Lys
  1               5                  10                  15 

Lys Arg Phe Ala Thr Ile Thr Ala Tyr Asp Tyr Ser Phe Ala Lys Leu
             20                  25                  30 

Phe Ala Asp Glu Gly Leu Asn Val Met Leu Val Gly Asp Ser Leu Gly
         35                  40                  45 

Met Thr Val Gln Gly His Asp Ser Thr Leu Pro Val Thr Val Ala Asp
     50                  55                  60 

Ile Ala Tyr His Thr Ala Ala Val Arg Arg Gly Ala Pro Asn Cys Leu
 65                  70                  75                  80 

Leu Leu Ala Asp Leu Pro Phe Met Ala Tyr Ala Thr Pro Glu Gln Ala
                 85                  90                  95 

Phe Glu Asn Ala Ala Thr Val Met Arg Ala Gly Ala Asn Met Val Lys
            100                 105                 110 

Ile Glu Gly Ala Glu Trp Leu Val Glu Thr Val Gln Met Leu Thr Glu
        115                 120                 125 

Arg Ala Val Pro Val Cys Gly His Leu Gly Leu Thr Pro Gln Ser Val
    130                 135                 140 

Asn Ile Phe Gly Gly Tyr Lys Val Gln Gly Arg Gly Asp Glu Leu Gly
145                 150                 155                 160 

Asp Gln Leu Leu Ser Asp Ala Leu Ala Leu Glu Ala Ala Gly Ala Gln
                165                 170                 175 

Leu Leu Val Leu Glu Cys Val Pro Val Glu Leu Ala Lys Arg Ile Thr
            180                 185                 190 

Glu Ala Leu Ala Ile Pro Val Ile Gly Ile Gly Ala Gly Asn Val Thr
        195                 200                 205 

Asp Gly Gln Ile Leu Val Met His Asp Ala Phe Gly Ile Thr Gly Gly
    210                 215                 220 

His Ile Pro Lys Phe Ala Lys Asn Phe Leu Ala Glu Thr Gly Asp Ile
225                 230                 235                 240 

Arg Ala Ala Val Arg Gln Tyr Met Ala Glu Val Glu Ser Gly Val Tyr
                245                 250                 255 

Pro Gly Glu Glu His Ser Phe His
            260                
