                         SEQUENCE LISTING

<110>  人֥ҩɷ޹˾
 
<120>  LAG-3ںϵͻ弰ƱӦ

<130>  IP210239

<150>  202110503020.0
<151>  2021-05-08

<160>  64    

<170>  PatentIn version 3.5

<210>  1
<211>  217
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-Fcİ

<400>  1

Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
1               5                   10                  15      


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
            20                  25                  30          


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
        35                  40                  45              


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
    50                  55                  60                  


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
65                  70                  75                  80  


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
                85                  90                  95      


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
            100                 105                 110         


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu 
        115                 120                 125             


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
    130                 135                 140                 


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
145                 150                 155                 160 


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
                165                 170                 175     


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
            180                 185                 190         


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
        195                 200                 205             


Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    210                 215         


<210>  2
<211>  45
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-c-JUN-His

<400>  2

Arg Ile Ala Arg Leu Glu Glu Lys Val Lys Thr Leu Lys Ala Gln Asn 
1               5                   10                  15      


Ser Glu Leu Ala Ser Thr Ala Asn Met Leu Arg Glu Gln Val Ala Gln 
            20                  25                  30          


Leu Lys Gln Lys Val Met Asn His His His His His His 
        35                  40                  45  


<210>  3
<211>  45
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-c-FOS-His

<400>  3

Leu Thr Asp Thr Leu Gln Ala Glu Thr Asp Gln Leu Glu Asp Glu Lys 
1               5                   10                  15      


Ser Ala Leu Gln Thr Glu Ile Ala Asn Leu Leu Lys Glu Lys Glu Lys 
            20                  25                  30          


Leu Glu Phe Ile Leu Ala Ala His His His His His His 
        35                  40                  45  


<210>  4
<211>  214
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹--PD-L1 FabVL-CL

<400>  4

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro Ala 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  5
<211>  221
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-ii-PD-L1 FabVH-CH1

<400>  5

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ser 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys 
    210                 215                 220     


<210>  6
<211>  25
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-Linkerİ

<400>  6

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Pro Lys Ser Cys Asp 
1               5                   10                  15      


Lys Thr His Thr Cys Pro Pro Cys Pro 
            20                  25  


<210>  7
<211>  25
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-Linker1İ

<400>  7

Asp Asp Asp Asp Lys Gly Ser Gly Ser Gly Glu Pro Lys Ser Cys Asp 
1               5                   10                  15      


Lys Thr His Thr Cys Pro Pro Cys Pro 
            20                  25  


<210>  8
<211>  25
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-Linker2İ

<400>  8

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Pro Lys Ser Ser Asp 
1               5                   10                  15      


Lys Thr His Thr Cys Pro Pro Cys Pro 
            20                  25  


<210>  9
<211>  15
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-Linker3İ

<400>  9

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  10
<211>  149
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-ҰLAG-3ṹD1İ

<400>  10

Leu Gln Pro Gly Ala Glu Val Pro Val Val Trp Ala Gln Glu Gly Ala 
1               5                   10                  15      


Pro Ala Gln Leu Pro Cys Ser Pro Thr Ile Pro Leu Gln Asp Leu Ser 
            20                  25                  30          


Leu Leu Arg Arg Ala Gly Val Thr Trp Gln His Gln Pro Asp Ser Gly 
        35                  40                  45              


Pro Pro Ala Ala Ala Pro Gly His Pro Leu Ala Pro Gly Pro His Pro 
    50                  55                  60                  


Ala Ala Pro Ser Ser Trp Gly Pro Arg Pro Arg Arg Tyr Thr Val Leu 
65                  70                  75                  80  


Ser Val Gly Pro Gly Gly Leu Arg Ser Gly Arg Leu Pro Leu Gln Pro 
                85                  90                  95      


Arg Val Gln Leu Asp Glu Arg Gly Arg Gln Arg Gly Asp Phe Ser Leu 
            100                 105                 110         


Trp Leu Arg Pro Ala Arg Arg Ala Asp Ala Gly Glu Tyr Arg Ala Ala 
        115                 120                 125             


Val His Leu Arg Asp Arg Ala Leu Ser Cys Arg Leu Arg Leu Arg Leu 
    130                 135                 140                 


Gly Gln Ala Ser Met 
145                 


<210>  11
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-ҰLAG-3ṹD2İ

<400>  11

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  12
<211>  91
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-ҰLAG-3ṹD3İ

<400>  12

Leu Glu Pro Pro Thr Pro Leu Thr Val Tyr Ala Gly Ala Gly Ser Arg 
1               5                   10                  15      


Val Gly Leu Pro Cys Arg Leu Pro Ala Gly Val Gly Thr Arg Ser Phe 
            20                  25                  30          


Leu Thr Ala Lys Trp Thr Pro Pro Gly Gly Gly Pro Asp Leu Leu Val 
        35                  40                  45              


Thr Gly Asp Asn Gly Asp Phe Thr Leu Arg Leu Glu Asp Val Ser Gln 
    50                  55                  60                  


Ala Gln Ala Gly Thr Tyr Thr Cys His Ile His Leu Gln Glu Gln Gln 
65                  70                  75                  80  


Leu Asn Ala Thr Val Thr Leu Ala Ile Ile Thr 
                85                  90      


<210>  13
<211>  82
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-ҰLAG-3ṹD4İ

<400>  13

Val Thr Pro Lys Ser Phe Gly Ser Pro Gly Ser Leu Gly Lys Leu Leu 
1               5                   10                  15      


Cys Glu Val Thr Pro Val Ser Gly Gln Glu Arg Phe Val Trp Ser Ser 
            20                  25                  30          


Leu Asp Thr Pro Ser Gln Arg Ser Phe Ser Gly Pro Trp Leu Glu Ala 
        35                  40                  45              


Gln Glu Ala Gln Leu Leu Ser Gln Pro Trp Gln Cys Gln Leu Tyr Gln 
    50                  55                  60                  


Gly Glu Arg Leu Leu Gly Ala Ala Val Tyr Phe Thr Glu Leu Ser Ser 
65                  70                  75                  80  


Pro Gly 
        


<210>  14
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-R188Aİ

<400>  14

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Ala Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  15
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-R192Aİ

<400>  15

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Ala Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  16
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-H196Aİ

<400>  16

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro Ala His 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  17
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-H197Aİ

<400>  17

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His Ala 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  18
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-P175Aİ

<400>  18

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Ala Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  19
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-S177Aİ

<400>  19

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ala Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  20
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-V178Aİ

<400>  20

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Ala His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  21
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-N183Aİ

<400>  21

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Ala Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  22
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-G185Aİ

<400>  22

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Ala Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  23
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-Q186Aİ

<400>  23

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Ala Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  24
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-G187Aİ

<400>  24

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Ala Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  25
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-V189Aİ

<400>  25

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Ala Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  26
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-P190Aİ

<400>  26

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Ala Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  27
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-P195Aİ

<400>  27

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Ala His His 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  28
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-L199Aİ

<400>  28

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Ala Ala Glu Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  29
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-F203Aİ

<400>  29

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Glu Ser Ala Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  30
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-Q208Aİ

<400>  30

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Pro Ala Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  31
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-S210Aİ

<400>  31

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Pro Gln Val Ala Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  32
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-P211Aİ

<400>  32

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Pro Gln Val Ser Ala Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  33
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-M212Aİ

<400>  33

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Ala Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  34
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-S214Aİ

<400>  34

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ala Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  35
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-P216Aİ

<400>  35

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Ala Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  36
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-G218Aİ

<400>  36

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Ala Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  37
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-H198Gİ

<400>  37

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


Gly Leu Ala Glu Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  38
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-H198Lİ

<400>  38

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


Leu Leu Ala Glu Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  39
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-H198Mİ

<400>  39

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


Met Leu Ala Glu Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  40
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-H198Wİ

<400>  40

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


Trp Leu Ala Glu Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  41
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-H198Yİ

<400>  41

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


Tyr Leu Ala Glu Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  42
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-H198Vİ

<400>  42

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


Val Leu Ala Glu Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  43
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-E201Rİ

<400>  43

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Arg Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  44
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-E201Nİ

<400>  44

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Asn Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  45
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-E201Dİ

<400>  45

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Asp Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  46
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-E201Qİ

<400>  46

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Gln Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  47
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-E201Hİ

<400>  47

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala His Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  48
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-E201Gİ

<400>  48

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Gly Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  49
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-E201Fİ

<400>  49

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Phe Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  50
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-E201Sİ

<400>  50

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Ser Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  51
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-P207R

<400>  51

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Arg Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  52
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-P207D

<400>  52

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Asp Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  53
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-P207E

<400>  53

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Glu Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  54
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-P207I

<400>  54

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Ile Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  55
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-P207M

<400>  55

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Met Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  56
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-P207S

<400>  56

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Ser Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  57
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-P207T

<400>  57

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Thr Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  58
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-P207Y

<400>  58

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Tyr Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  59
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-D2ṹͻD2-V209T

<400>  59

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Pro Gln Thr Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


<210>  60
<211>  218
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹--PD-1 FabVL-CL

<400>  60

Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Lys Gly Val Ser Thr Ser 
            20                  25                  30          


Gly Tyr Ser Tyr Leu His Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro 
        35                  40                  45              


Arg Leu Leu Ile Tyr Leu Ala Ser Tyr Leu Glu Ser Gly Val Pro Ala 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln His Ser Arg 
                85                  90                  95      


Asp Leu Pro Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105                 110         


Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln 
        115                 120                 125             


Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr 
    130                 135                 140                 


Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser 
145                 150                 155                 160 


Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr 
                165                 170                 175     


Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys 
            180                 185                 190         


His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro 
        195                 200                 205             


Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215             


<210>  61
<211>  223
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹--PD-1 FabVH-CH1

<400>  61

Gln Val Gln Leu Val Gln Ser Gly Val Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Tyr Met Tyr Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Gly Ile Asn Pro Ser Asn Gly Gly Thr Asn Phe Asn Glu Lys Phe 
    50                  55                  60                  


Lys Asn Arg Val Thr Leu Thr Thr Asp Ser Ser Thr Thr Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Lys Ser Leu Gln Phe Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Asp Tyr Arg Phe Asp Met Gly Phe Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Thr Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val 
        115                 120                 125             


Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala 
    130                 135                 140                 


Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser 
145                 150                 155                 160 


Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val 
                165                 170                 175     


Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro 
            180                 185                 190         


Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys 
        195                 200                 205             


Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys 
    210                 215                 220             


<210>  62
<211>  239
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-ҰLAG-3D1-D2ṹ򰱻

<400>  62

Leu Gln Pro Gly Ala Glu Val Pro Val Val Trp Ala Gln Glu Gly Ala 
1               5                   10                  15      


Pro Ala Gln Leu Pro Cys Ser Pro Thr Ile Pro Leu Gln Asp Leu Ser 
            20                  25                  30          


Leu Leu Arg Arg Ala Gly Val Thr Trp Gln His Gln Pro Asp Ser Gly 
        35                  40                  45              


Pro Pro Ala Ala Ala Pro Gly His Pro Leu Ala Pro Gly Pro His Pro 
    50                  55                  60                  


Ala Ala Pro Ser Ser Trp Gly Pro Arg Pro Arg Arg Tyr Thr Val Leu 
65                  70                  75                  80  


Ser Val Gly Pro Gly Gly Leu Arg Ser Gly Arg Leu Pro Leu Gln Pro 
                85                  90                  95      


Arg Val Gln Leu Asp Glu Arg Gly Arg Gln Arg Gly Asp Phe Ser Leu 
            100                 105                 110         


Trp Leu Arg Pro Ala Arg Arg Ala Asp Ala Gly Glu Tyr Arg Ala Ala 
        115                 120                 125             


Val His Leu Arg Asp Arg Ala Leu Ser Cys Arg Leu Arg Leu Arg Leu 
    130                 135                 140                 


Gly Gln Ala Ser Met Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser 
145                 150                 155                 160 


Asp Trp Val Ile Leu Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala 
                165                 170                 175     


Ser Val His Trp Phe Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg 
            180                 185                 190         


Glu Ser Pro His His His Leu Ala Glu Ser Phe Leu Phe Leu Pro Gln 
        195                 200                 205             


Val Ser Pro Met Asp Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg 
    210                 215                 220                 


Asp Gly Phe Asn Val Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
225                 230                 235                 


<210>  63
<211>  525
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-LAG-3Уźģ

<400>  63

Met Trp Glu Ala Gln Phe Leu Gly Leu Leu Phe Leu Gln Pro Leu Trp 
1               5                   10                  15      


Val Ala Pro Val Lys Pro Leu Gln Pro Gly Ala Glu Val Pro Val Val 
            20                  25                  30          


Trp Ala Gln Glu Gly Ala Pro Ala Gln Leu Pro Cys Ser Pro Thr Ile 
        35                  40                  45              


Pro Leu Gln Asp Leu Ser Leu Leu Arg Arg Ala Gly Val Thr Trp Gln 
    50                  55                  60                  


His Gln Pro Asp Ser Gly Pro Pro Ala Ala Ala Pro Gly His Pro Leu 
65                  70                  75                  80  


Ala Pro Gly Pro His Pro Ala Ala Pro Ser Ser Trp Gly Pro Arg Pro 
                85                  90                  95      


Arg Arg Tyr Thr Val Leu Ser Val Gly Pro Gly Gly Leu Arg Ser Gly 
            100                 105                 110         


Arg Leu Pro Leu Gln Pro Arg Val Gln Leu Asp Glu Arg Gly Arg Gln 
        115                 120                 125             


Arg Gly Asp Phe Ser Leu Trp Leu Arg Pro Ala Arg Arg Ala Asp Ala 
    130                 135                 140                 


Gly Glu Tyr Arg Ala Ala Val His Leu Arg Asp Arg Ala Leu Ser Cys 
145                 150                 155                 160 


Arg Leu Arg Leu Arg Leu Gly Gln Ala Ser Met Thr Ala Ser Pro Pro 
                165                 170                 175     


Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu Asn Cys Ser Phe Ser 
            180                 185                 190         


Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe Arg Asn Arg Gly Gln 
        195                 200                 205             


Gly Arg Val Pro Val Arg Glu Ser Pro His His His Leu Ala Glu Ser 
    210                 215                 220                 


Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp Ser Gly Pro Trp Gly 
225                 230                 235                 240 


Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val Ser Ile Met Tyr Asn 
                245                 250                 255     


Leu Thr Val Leu Gly Leu Glu Pro Pro Thr Pro Leu Thr Val Tyr Ala 
            260                 265                 270         


Gly Ala Gly Ser Arg Val Gly Leu Pro Cys Arg Leu Pro Ala Gly Val 
        275                 280                 285             


Gly Thr Arg Ser Phe Leu Thr Ala Lys Trp Thr Pro Pro Gly Gly Gly 
    290                 295                 300                 


Pro Asp Leu Leu Val Thr Gly Asp Asn Gly Asp Phe Thr Leu Arg Leu 
305                 310                 315                 320 


Glu Asp Val Ser Gln Ala Gln Ala Gly Thr Tyr Thr Cys His Ile His 
                325                 330                 335     


Leu Gln Glu Gln Gln Leu Asn Ala Thr Val Thr Leu Ala Ile Ile Thr 
            340                 345                 350         


Val Thr Pro Lys Ser Phe Gly Ser Pro Gly Ser Leu Gly Lys Leu Leu 
        355                 360                 365             


Cys Glu Val Thr Pro Val Ser Gly Gln Glu Arg Phe Val Trp Ser Ser 
    370                 375                 380                 


Leu Asp Thr Pro Ser Gln Arg Ser Phe Ser Gly Pro Trp Leu Glu Ala 
385                 390                 395                 400 


Gln Glu Ala Gln Leu Leu Ser Gln Pro Trp Gln Cys Gln Leu Tyr Gln 
                405                 410                 415     


Gly Glu Arg Leu Leu Gly Ala Ala Val Tyr Phe Thr Glu Leu Ser Ser 
            420                 425                 430         


Pro Gly Ala Gln Arg Ser Gly Arg Ala Pro Gly Ala Leu Pro Ala Gly 
        435                 440                 445             


His Leu Leu Leu Phe Leu Ile Leu Gly Val Leu Ser Leu Leu Leu Leu 
    450                 455                 460                 


Val Thr Gly Ala Phe Gly Phe His Leu Trp Arg Arg Gln Trp Arg Pro 
465                 470                 475                 480 


Arg Arg Phe Ser Ala Leu Glu Gln Gly Ile His Pro Pro Gln Ala Gln 
                485                 490                 495     


Ser Lys Ile Glu Glu Leu Glu Gln Glu Pro Glu Pro Glu Pro Glu Pro 
            500                 505                 510         


Glu Pro Glu Pro Glu Pro Glu Pro Glu Pro Glu Gln Leu 
        515                 520                 525 


<210>  64
<211>  90
<212>  PRT
<213>  ˹

<220>
<223>  ϳɵ˹-LAG-3D2ṹͻP172A

<400>  64

Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu 
1               5                   10                  15      


Asn Cys Ser Phe Ser Arg Ala Asp Arg Pro Ala Ser Val His Trp Phe 
            20                  25                  30          


Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg Glu Ser Pro His His 
        35                  40                  45              


His Leu Ala Glu Ser Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp 
    50                  55                  60                  


Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val 
65                  70                  75                  80  


Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
                85                  90  


