                         SEQUENCE LISTING

<110>  The Trustees of Indiana University
 
<120>  MOLECULAR DESIGN OF GLUCOSE SENSORS IN GLUCOSE-RESPONSIVE INSULIN
        ANALOGUES

<130>  29920-354919

<150>  63/183,325
<151>  2021-05-03

<160>  140   

<170>  PatentIn version 3.5

<210>  1
<211>  86
<212>  PRT
<213>  Homo sapiens

<400>  1

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Arg Arg 
            20                  25                  30          


Glu Ala Glu Asp Leu Gln Val Gly Gln Val Glu Leu Gly Gly Gly Pro 
        35                  40                  45              


Gly Ala Gly Ser Leu Gln Pro Leu Ala Leu Glu Gly Ser Leu Gln Lys 
    50                  55                  60                  


Arg Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln 
65                  70                  75                  80  


Leu Glu Asn Tyr Cys Asn 
                85      


<210>  2
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  2

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn 
            20      


<210>  3
<211>  30
<212>  PRT
<213>  Homo sapiens

<400>  3

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr 
            20                  25                  30  


<210>  4
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is any amino acid or a modified amino acid 
       comprising a glucose binding element linked to its side chain

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa at position 3 is any amino acid or a modified amino acid 
       comprising a glucose binding element linked to its side chain

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is Cys or selenocysteine

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is Thr, His, Lys, Arg, or Glu

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  Xaa at position 13 is Cys or selenocysteine

<400>  4

Xaa Xaa Xaa Ile Val Glu Gln Xaa Cys Xaa Ser Ile Xaa Ser Leu Tyr 
1               5                   10                  15      


Gln Leu Glu Asn Tyr Cys Asn 
            20              


<210>  5
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is any amino acid or a modified amino acid 
       comprising a glucose binding element linked to its side chain

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is any amino acid or a modified amino acid 
       comprising a glucose binding element linked to its side chain

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa at position 7 is Cys or selenocysteine

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa at position 9 is Thr, His, Lys, Arg, or Glu

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Xaa at position 12 is Cys or selenocysteine

<400>  5

Xaa Xaa Ile Val Glu Gln Xaa Cys Xaa Ser Ile Xaa Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  6
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa at position 3 is Lys or Asn

<220>
<221>  MISC_FEATURE
<222>  (28)..(28)
<223>  Xaa at position 28 is Orn, Glu Asp, Lys, Pro, or a modified amino
       acid comprising a diol bearing moiety linked to the side chain of
       the amino acid

<220>
<221>  MISC_FEATURE
<222>  (29)..(29)
<223>  Xaa at position 29 is Orn, Lys, Pro, or a modified amino acid 
       comprising a diol bearing moiety linked to the side chain of the 
       amino acid

<220>
<221>  MISC_FEATURE
<222>  (30)..(30)
<223>  Xaa at position 30 is Thr, or a diol bearing amino acid

<400>  6

Phe Val Xaa Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Xaa Xaa Xaa 
            20                  25                  30  


<210>  7
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa at position 3 is Lys or Asn

<220>
<221>  MISC_FEATURE
<222>  (28)..(28)
<223>  Xaa at position 28 is a diol bearing amino acid

<400>  7

Phe Val Xaa Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Xaa 
            20                  25              


<210>  8
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Cys comprising a glucose binding element 
       linked to its side chain

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is selenocysteine

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is His

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  Xaa at position 13 is selenocysteine

<400>  8

Gly Xaa Gly Ile Val Glu Gln Xaa Cys Xaa Ser Ile Xaa Ser Leu Tyr 
1               5                   10                  15      


Gln Leu Glu Asn Tyr Cys Asn 
            20              


<210>  9
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog

<400>  9

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg 
            20          


<210>  10
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is a modified Lys comprising a diol bearing 
       moiety linked to the side chain of the amino acid

<400>  10

Gly Phe Phe Tyr Thr Xaa Pro Thr 
1               5               


<210>  11
<211>  50
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (30)..(30)
<223>  Xaa at position 30 is D-Dap(Pr)

<400>  11

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Lys Gly Xaa Ile Val 
            20                  25                  30          


Glu Gln Cys Cys His Ser Ile Cys Ser Leu Tyr Gln Leu Glu Asn Tyr 
        35                  40                  45              


Cys Asn 
    50  


<210>  12
<211>  51
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (30)..(30)
<223>  Xaa at position 30 is D-Dap(Pr)

<400>  12

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Lys Gly Xaa Gly Ile 
            20                  25                  30          


Val Glu Gln Cys Cys His Ser Ile Cys Ser Leu Tyr Gln Leu Glu Asn 
        35                  40                  45              


Tyr Cys Asn 
    50      


<210>  13
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is a modified D-Cys, L-Cys, D-homoCys, 
       L-homoCys, D-Asp, L-Asp, D-Glu, L-Glu, L-Lys, D-Lys, L-Orn, 
       D-Orn, L-Dab, D-Dap, and L-Dap,wherein the modified amino acid 
       comprises a glucose-binding elements linked to the side chain of 
       

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is Cys or selenocysteine

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is Thr, His, Lys, Arg, or Glu

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  Xaa at position 13 is Cys or selenocysteine

<220>
<221>  MISC_FEATURE
<222>  (23)..(23)
<223>  Xaa at position 23 is asparagine, serine, glycine or alanine

<400>  13

Gly Xaa Gly Ile Val Glu Gln Xaa Cys Xaa Ser Ile Xaa Ser Leu Tyr 
1               5                   10                  15      


Gln Leu Glu Asn Tyr Cys Xaa 
            20              


<210>  14
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is a modified L-homoCys, D-homoCys, L-Asp, 
       D-Asp, L-Glu, D-Glu, LDap, D-Dap, L-Cys, L-Lys, D-Lys, L-Orn, 
       D-Orn, L-Dab, and D-Dab, wherein the modified amino acid 
       comprises a glucose-binding elements linked to the side chain of 
       

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa at position 7 is Cys or selenocysteine

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa at position 9 is Thr, His, Lys, Arg, or Glu

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Xaa at position 12 is Cys or selenocysteine

<220>
<221>  MISC_FEATURE
<222>  (22)..(22)
<223>  Xaa at position 22 is asparagine, serine, glycine or alanine

<400>  14

Xaa Gly Ile Val Glu Gln Xaa Cys Xaa Ser Ile Xaa Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  15
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is a modified L-homoCys, D-homoCys, L-Asp, 
       D-Asp, L-Glu, D-Glu, LDap, D-Dap, L-Cys, L-Lys, D-Lys, L-Orn, 
       D-Orn, L-Dab, and D-Dab, wherein the modified amino acid 
       comprises a glucose-binding elements linked to the side chain of 
       

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa at position 7 is Cys or selenocysteine

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa at position 9 is Thr, His, Lys, Arg, or Glu

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Xaa at position 12 is Cys or selenocysteine

<220>
<221>  MISC_FEATURE
<222>  (22)..(22)
<223>  Xaa at position 22 is asparagine, serine, glycine or alanine

<400>  15

Gly Xaa Ile Val Glu Gln Xaa Cys Xaa Ser Ile Xaa Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Xaa 
            20          


<210>  16
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is a modified L-Cys, L-Lys, D-Lys, L-Orn, 
       D-Orn, L-Dab, and D-Dab, wherein the modified amino acid 
       comprises a glucose-binding elements linked to the side chain of 
       the amino acid

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is Cys or selenocysteine

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is Thr, His, Lys, Arg, or Glu

<220>
<221>  MISC_FEATURE
<222>  (11)..(11)
<223>  Xaa at position 11 is Cys or selenocysteine

<400>  16

Xaa Ile Val Glu Gln Xaa Cys Xaa Ser Ile Xaa Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn 
            20      


<210>  17
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa at position 3 is Lys or Asn

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa at position 5 is histidine or threonine

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa at position 9 is alanine, glycine or serine

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is histidine, aspartic acid or glutamic acid

<400>  17

Phe Val Xaa Gln Xaa Leu Cys Gly Xaa Xaa Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe 
            20                  25  


<210>  18
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa at position 3 is proline, aspartic acid or glutamic acid

<400>  18

Tyr Thr Xaa Lys Thr 
1               5   


<210>  19
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa at position 3 is proline, aspartic acid or glutamic acid

<400>  19

Tyr Thr Xaa Lys Pro 
1               5   


<210>  20
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog

<400>  20

Tyr Thr Lys Pro Thr 
1               5   


<210>  21
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog

<400>  21

Tyr Thr Lys Pro Thr Arg 
1               5       


<210>  22
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog

<400>  22

Tyr Thr Lys Pro Thr Arg Arg 
1               5           


<210>  23
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa at position 3 is proline, aspartic acid or glutamic acid

<400>  23

Tyr Thr Xaa Lys 
1               


<210>  24
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog

<400>  24

Tyr Thr Lys Pro 
1               


<210>  25
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog

<400>  25

Tyr Thr Pro Lys 
1               


<210>  26
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa at position 3 is Lys or Asn

<220>
<221>  MISC_FEATURE
<222>  (28)..(28)
<223>  Xaa at position 28 is proline, aspartic acid or glutamic acid

<400>  26

Phe Val Xaa Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Xaa Lys Pro 
            20                  25                  30  


<210>  27
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is of threonine lysine, arginine, glutamic 
       acid, or histidine

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  Xaa at position 21 is asparagine, glycine and alanine

<400>  27

Gly Ile Val Glu Gln Cys Cys Xaa Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Xaa 
            20      


<210>  28
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa at position 5 is histidine or threonine

<400>  28

Phe Val Asn Gln Xaa Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Glu Lys Thr 
            20                  25                  30  


<210>  29
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa at position 5 is histidine or threonine

<400>  29

Phe Val Asn Gln Xaa Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Asp Lys Thr 
            20                  25                  30  


<210>  30
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa at position 5 is histidine or threonine

<400>  30

Phe Val Asn Gln Xaa Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Lys Pro Thr 
            20                  25                  30  


<210>  31
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa at position 5 is histidine or threonine

<400>  31

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Lys Lys Pro 
            20                  25                  30  


<210>  32
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa at position 5 is histidine or threonine

<400>  32

Phe Val Asn Gln Xaa Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr 
            20                  25                  30  


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog

<400>  33
gttcaatgcc aggttcccag                                                   20


<210>  34
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog

<400>  34
ttgcaggcca gttgttgac                                                    19


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog

<400>  35
agagacaaga tccgcctgaa                                                   20


<210>  36
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog

<400>  36
cttccagtag ttccctcca                                                    19


<210>  37
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog

<400>  37
cgacatcgaa gacatgcttc ag                                                22


<210>  38
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog

<400>  38
ggaaggcttc aagagaggag c                                                 21


<210>  39
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog

<400>  39
atggtttaca tgttccaata t                                                 21


<210>  40
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog

<400>  40
atgaggtcca ccaccctggt tg                                                22


<210>  41
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide fragment


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is Lys, Orn, Dab, or Dap with sidechain linked 
       GBE 1-6

<400>  41

Gly Phe Phe Tyr Thr Xaa Pro Thr 
1               5               


<210>  42
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is cyteine with linked GBE

<400>  42

Gly Xaa Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr 
1               5                   10                  15      


Gln Leu Glu Asn Tyr Cys Asn 
            20              


<210>  43
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa at position 5 is histidine or threonine

<220>
<221>  MISC_FEATURE
<222>  (28)..(28)
<223>  Xaa at position 28 is a modfied lysine comprising DHBA

<400>  43

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Xaa Pro Thr 
            20                  25                  30  


<210>  44
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is dCys comprising a glucose binding element 
       linked to its side chain

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa at position 7 is selenocysteine

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Xaa at position 12 is selenocysteine

<400>  44

Gly Xaa Ile Val Glu Gln Xaa Cys Thr Ser Ile Xaa Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  45
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Cys comprising a glucose binding element 
       linked to its side chain

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is selenocysteine

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  Xaa at position 13 is selenocysteine

<400>  45

Gly Xaa Gly Ile Val Glu Gln Xaa Cys Thr Ser Ile Xaa Ser Leu Tyr 
1               5                   10                  15      


Gln Leu Glu Asn Tyr Cys Asn 
            20              


<210>  46
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is dCys comprising a glucose binding element 
       linked to its side chain

<400>  46

Gly Xaa Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr 
1               5                   10                  15      


Gln Leu Glu Asn Tyr Cys Asn 
            20              


<210>  47
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is dCys comprising a glucose binding element 
       linked to its side chain

<400>  47

Gly Xaa Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  48
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is dCys comprising a glucose binding element 
       linked to its side chain

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa at position 7 is selenocysteine

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Xaa at position 12 is selenocysteine

<400>  48

Gly Xaa Ile Val Glu Gln Xaa Cys Thr Ser Ile Xaa Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  49
<211>  30
<212>  PRT
<213>  Homo sapiens

<400>  49

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr 
            20                  25                  30  


<210>  50
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa at position 5 is histidine or threonine

<220>
<221>  MISC_FEATURE
<222>  (28)..(28)
<223>  Xaa at position 28 is a modfied lysine comprising DHBA

<400>  50

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Xaa Pro Thr 
            20                  25                  30  


<210>  51
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Cys comprising a glucose binding element 
       linked to its side chain

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is selenocysteine

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  Xaa at position 13 is selenocysteine

<400>  51

Gly Xaa Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr 
1               5                   10                  15      


Gln Leu Glu Asn Tyr Cys Asn 
            20              


<210>  52
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Cys comprising a glucose binding element 
       linked to its side chain

<400>  52

Xaa Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  53
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Cys comprising a glucose binding element 
       linked to its side chain

<400>  53

Xaa Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  54
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is dCys comprising a glucose binding element 
       linked to its side chain

<400>  54

Xaa Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  55
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is dCys comprising a glucose binding element 
       linked to its side chain

<400>  55

Xaa Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  56
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Dap comprising a glucose binding element 
       linked to its side chain

<400>  56

Gly Xaa Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr 
1               5                   10                  15      


Gln Leu Glu Asn Tyr Cys Asn 
            20              


<210>  57
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Dap comprising a glucose binding element 
       linked to its side chain

<400>  57

Gly Xaa Ile Val Glu Gln Cys Cys His Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  58
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is dDap comprising a glucose binding element 
       linked to its side chain

<400>  58

Gly Xaa Ile Val Glu Gln Cys Cys His Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  59
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is dDap comprising a glucose binding element 
       linked to its side chain

<400>  59

Gly Xaa Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  60
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is dOrn comprising a glucose binding element 
       linked to its side chain

<400>  60

Gly Xaa Ile Val Glu Gln Cys Cys His Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  61
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is dLys comprising a glucose binding element 
       linked to its side chain

<400>  61

Gly Xaa Ile Val Glu Gln Cys Cys His Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  62
<211>  30
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (30)..(30)
<223>  Xaa at position 30 is a diol bearing amino acid derivative

<400>  62

Phe Val Lys Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Glu Lys Xaa 
            20                  25                  30  


<210>  63
<211>  30
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (30)..(30)
<223>  Xaa at position 30 is a diol bearing amino acid derivative

<400>  63

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Asp Lys Xaa 
            20                  25                  30  


<210>  64
<211>  30
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (30)..(30)
<223>  Xaa at position 30 is a diol bearing amino acid derivative

<400>  64

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Lys Pro Xaa 
            20                  25                  30  


<210>  65
<211>  30
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (30)..(30)
<223>  Xaa at position 30 is a diol bearing amino acid derivative

<400>  65

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Xaa 
            20                  25                  30  


<210>  66
<211>  29
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (29)..(29)
<223>  Xaa at position 29 is a diol bearing amino acid derivative

<400>  66

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Lys Xaa 
            20                  25                  


<210>  67
<211>  30
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (29)..(29)
<223>  Xaa at position 29 is ornithine

<220>
<221>  MISC_FEATURE
<222>  (30)..(30)
<223>  Xaa at position 30 is a diol bearing amino acid derivative

<400>  67

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Xaa Xaa 
            20                  25                  30  


<210>  68
<211>  29
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (29)..(29)
<223>  Xaa at position 29 is a diol bearing amino acid derivative

<400>  68

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Xaa 
            20                  25                  


<210>  69
<211>  29
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (28)..(28)
<223>  Xaa at position 28 is ornithine

<220>
<221>  MISC_FEATURE
<222>  (29)..(29)
<223>  Xaa at position 29 is a diol bearing amino acid derivative

<400>  69

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Xaa Xaa 
            20                  25                  


<210>  70
<211>  28
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (28)..(28)
<223>  Xaa at position 28 is a diol bearing amino acid derivative

<400>  70

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Xaa 
            20                  25              


<210>  71
<211>  27
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (27)..(27)
<223>  Xaa at position 27 is a diol bearing amino acid derivative

<400>  71

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Xaa 
            20                  25          


<210>  72
<211>  27
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (26)..(26)
<223>  Xaa at position 26 is sarcosine

<220>
<221>  MISC_FEATURE
<222>  (27)..(27)
<223>  Xaa at position 27 is ADP

<400>  72

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Xaa Xaa 
            20                  25          


<210>  73
<211>  27
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (26)..(26)
<223>  Xaa at position 26 is dAla

<220>
<221>  MISC_FEATURE
<222>  (27)..(27)
<223>  Xaa at position 27 is ADP

<400>  73

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Xaa Xaa 
            20                  25          


<210>  74
<211>  28
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (28)..(28)
<223>  Xaa at position 28 is ADP

<400>  74

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Gly Xaa 
            20                  25              


<210>  75
<211>  31
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (31)..(31)
<223>  Xaa at position 31 is a diol bearing amino acid derivative

<400>  75

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Asp Lys Xaa Xaa 
            20                  25                  30      


<210>  76
<211>  31
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (31)..(31)
<223>  Xaa at position 31 is a diol bearing amino acid derivative

<400>  76

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Lys Pro Xaa Xaa 
            20                  25                  30      


<210>  77
<211>  31
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (31)..(31)
<223>  Xaa at position 31 is a diol bearing amino acid derivative

<400>  77

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Xaa Xaa 
            20                  25                  30      


<210>  78
<211>  32
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (30)..(31)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (32)..(32)
<223>  Xaa at position 32 is a diol bearing amino acid derivative

<400>  78

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Asp Lys Xaa Xaa Xaa 
            20                  25                  30          


<210>  79
<211>  32
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (30)..(31)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (32)..(32)
<223>  Xaa at position 32 is a diol bearing amino acid derivative

<400>  79

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Lys Pro Xaa Xaa Xaa 
            20                  25                  30          


<210>  80
<211>  32
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (30)..(31)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (32)..(32)
<223>  Xaa at position 32 is a diol bearing amino acid derivative

<400>  80

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Xaa Xaa Xaa 
            20                  25                  30          


<210>  81
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  81

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn 
            20      


<210>  82
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  82

Gly Ile Val Glu Gln Cys Cys His Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn 
            20      


<210>  83
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is D-amino acid comprising a glucose-binding 
       element linked to its side chain

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is cys or selenocysteine

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is Thr, His, Lys, Arg, or Glu

<220>
<221>  MISC_FEATURE
<222>  (11)..(11)
<223>  Xaa at position 11 is  cys or selenocysteine

<400>  83

Xaa Ile Val Glu Gln Xaa Cys Thr Ser Ile Xaa Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn 
            20      


<210>  84
<211>  51
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (30)..(30)
<223>  Xaa at Position 30 is Cys

<400>  84

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Lys Gly Xaa Gly Ile 
            20                  25                  30          


Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu Glu Asn 
        35                  40                  45              


Tyr Cys Asn 
    50      


<210>  85
<211>  51
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (30)..(30)
<223>  Xaa at Position 30 is Cys(GBE)

<400>  85

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Lys Gly Xaa Gly Ile 
            20                  25                  30          


Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu Glu Asn 
        35                  40                  45              


Tyr Cys Asn 
    50      


<210>  86
<211>  22
<212>  PRT
<213>  Homo sapiens

<400>  86

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg 
            20          


<210>  87
<211>  23
<212>  PRT
<213>  Homo sapiens

<400>  87

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly 
            20              


<210>  88
<211>  30
<212>  PRT
<213>  Homo sapiens

<400>  88

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Lys Pro Thr 
            20                  25                  30  


<210>  89
<211>  30
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (28)..(28)
<223>  Xaa at position 28 is Lys(DHBA)

<400>  89

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Xaa Pro Thr 
            20                  25                  30  


<210>  90
<211>  28
<212>  PRT
<213>  Homo sapiens

<400>  90

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Lys 
            20                  25              


<210>  91
<211>  30
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (28)..(28)
<223>  Xaa at position 28 is Orn(DHBA)

<400>  91

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Xaa Pro Thr 
            20                  25                  30  


<210>  92
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Cys(GBE)

<400>  92

Gly Xaa Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr 
1               5                   10                  15      


Gln Leu Glu Asn Tyr Cys Asn 
            20              


<210>  93
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Cys(GBE)

<400>  93

Gly Xaa Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  94
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Cys(GBE)

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is selenocysteine

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  Xaa at position 13 is selenocysteine

<400>  94

Gly Xaa Gly Ile Val Glu Gln Xaa Cys Thr Ser Ile Xaa Ser Leu Tyr 
1               5                   10                  15      


Gln Leu Glu Asn Tyr Cys Asn 
            20              


<210>  95
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is dCys(GBE)

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa at position 7 is selenocysteine

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Xaa at position 12 is selenocysteine

<400>  95

Gly Xaa Ile Val Glu Gln Xaa Cys Thr Ser Ile Xaa Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  96
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is Cys(GBE)

<400>  96

Xaa Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  97
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is dCys(GBE)

<400>  97

Gly Xaa Ile Val Glu Gln Cys Cys His Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  98
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is D-Dap(Mtt)

<400>  98

Gly Xaa Ile Val Glu Gln Cys Cys His Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  99
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is D-Dap(Fmoc-Dap(Fmoc))

<400>  99

Gly Xaa Ile Val Glu Gln Cys Cys His Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  100
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is D-Dap(H2N-Dap(NH2))

<400>  100

Gly Xaa Ile Val Glu Gln Cys Cys His Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  101
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is D-Dap(BCM2-Dap(BCM2))

<400>  101

Gly Xaa Ile Val Glu Gln Cys Cys His Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  102
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is D-Dap(NH2)

<400>  102

Gly Xaa Ile Val Glu Gln Cys Cys His Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  103
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is D-Dap(Fmoc-Dap(Mtt))

<400>  103

Gly Xaa Ile Val Glu Gln Cys Cys His Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  104
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is D-Dap(H2N-Dap(Mtt))

<400>  104

Gly Xaa Ile Val Glu Gln Cys Cys His Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  105
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is D-Dap(Gly-Dap(NH2))

<400>  105

Gly Xaa Ile Val Glu Gln Cys Cys His Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  106
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is D-Dap(H2N-Dap(FmocG))

<400>  106

Gly Xaa Ile Val Glu Gln Cys Cys His Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  107
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is D-Dap(BCM2-Gly-Dap(BCM2))

<400>  107

Gly Xaa Ile Val Glu Gln Cys Cys His Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  108
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is D-Dap(GBE-Gly-Dap(GBE))

<400>  108

Gly Xaa Ile Val Glu Gln Cys Cys His Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  109
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Dap(alloc)

<400>  109

Gly Xaa Ile Val Glu Gln Cys Cys His Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  110
<211>  23
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is dCys

<400>  110

Gly Xaa Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr 
1               5                   10                  15      


Gln Leu Glu Asn Tyr Cys Asn 
            20              


<210>  111
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is dCys

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is selenocysteine

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  Xaa at position 13 is selenocysteine

<400>  111

Gly Xaa Gly Ile Val Glu Gln Xaa Cys Thr Ser Ile Xaa Ser Leu Tyr 
1               5                   10                  15      


Gln Leu Glu Asn Tyr Cys Asn 
            20              


<210>  112
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is dCys

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa at position 7 is selenocysteine

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Xaa at position 12 is selenocysteine

<400>  112

Gly Xaa Ile Val Glu Gln Xaa Cys Thr Ser Ile Xaa Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  113
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is Cys(Acm)

<400>  113

Xaa Gly Ile Val Glu Gln Cys Cys His Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  114
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Cys(Npys)

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is selenocysteine

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  Xaa at position 13 is selenocysteine

<400>  114

Gly Xaa Gly Ile Val Glu Gln Xaa Cys Thr Ser Ile Xaa Ser Leu Tyr 
1               5                   10                  15      


Gln Leu Glu Asn Tyr Cys Asn 
            20              


<210>  115
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Cys

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is selenocysteine

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  Xaa at position 13 is selenocysteine

<400>  115

Gly Xaa Gly Ile Val Glu Gln Xaa Cys Thr Ser Ile Xaa Ser Leu Tyr 
1               5                   10                  15      


Gln Leu Glu Asn Tyr Cys Asn 
            20              


<210>  116
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is dCys(Npys)

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is selenocysteine

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  Xaa at position 13 is selenocysteine

<400>  116

Gly Xaa Gly Ile Val Glu Gln Xaa Cys Thr Ser Ile Xaa Ser Leu Tyr 
1               5                   10                  15      


Gln Leu Glu Asn Tyr Cys Asn 
            20              


<210>  117
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is dCys

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is selenocysteine

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  Xaa at position 13 is selenocysteine

<400>  117

Gly Xaa Gly Ile Val Glu Gln Xaa Cys Thr Ser Ile Xaa Ser Leu Tyr 
1               5                   10                  15      


Gln Leu Glu Asn Tyr Cys Asn 
            20              


<210>  118
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is dCys(Acm)

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is selenocysteine

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  Xaa at position 13 is selenocysteine

<400>  118

Gly Xaa Gly Ile Val Glu Gln Xaa Cys Thr Ser Ile Xaa Ser Leu Tyr 
1               5                   10                  15      


Gln Leu Glu Asn Tyr Cys Asn 
            20              


<210>  119
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is dCys(GBE)

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa at position 7 is selenocysteine

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Xaa at position 12 is selenocysteine

<400>  119

Gly Xaa Ile Val Glu Gln Xaa Cys Thr Ser Ile Xaa Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  120
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Dap(GBE)

<400>  120

Gly Xaa Ile Val Glu Gln Cys Cys His Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  121
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Dap(GBE)

<400>  121

Gly Xaa Ile Val Glu Gln Cys Cys His Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  122
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is DOrn(GBE)

<400>  122

Gly Xaa Ile Val Glu Gln Cys Cys His Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  123
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Orn(GBE)

<400>  123

Gly Xaa Gly Ile Val Glu Gln Cys Cys His Ser Ile Cys Ser Leu Tyr 
1               5                   10                  15      


Gln Leu Glu Asn Tyr Cys Asn 
            20              


<210>  124
<211>  30
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (28)..(28)
<223>  Xaa at position 28 is Dap(GBE)

<400>  124

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Xaa Pro Thr 
            20                  25                  30  


<210>  125
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is DDap(GBE)

<400>  125

Gly Xaa Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  126
<211>  28
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (27)..(27)
<223>  Xaa at position 27 is Gly[APD]

<220>
<221>  MISC_FEATURE
<222>  (28)..(28)
<223>  Xaa at position 28 is Gly[APD]

<400>  126

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Xaa Xaa 
            20                  25              


<210>  127
<211>  30
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (28)..(28)
<223>  Xaa at position 28 is K(GBE1-CAc)

<400>  127

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Xaa Pro Thr 
            20                  25                  30  


<210>  128
<211>  30
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (28)..(28)
<223>  Xaa at position 28 is Orn(GBE)

<400>  128

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Xaa Pro Thr 
            20                  25                  30  


<210>  129
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is dDap(DHBA)

<400>  129

Gly Xaa Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  130
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Dap(DHBA)

<400>  130

Gly Xaa Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr 
1               5                   10                  15      


Gln Leu Glu Asn Tyr Cys Asn 
            20              


<210>  131
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Orn(DHBA)

<400>  131

Gly Xaa Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr 
1               5                   10                  15      


Gln Leu Glu Asn Tyr Cys Asn 
            20              


<210>  132
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is dOrn(DHBA)

<400>  132

Gly Xaa Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  133
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is DOPA

<400>  133

Gly Xaa Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr 
1               5                   10                  15      


Gln Leu Glu Asn Tyr Cys Asn 
            20              


<210>  134
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is N(APD)

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is DOPA

<400>  134

Xaa Xaa Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr 
1               5                   10                  15      


Gln Leu Glu Asn Tyr Cys Asn 
            20              


<210>  135
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is DOPA

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is N(APD)

<400>  135

Xaa Xaa Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr 
1               5                   10                  15      


Gln Leu Glu Asn Tyr Cys Asn 
            20              


<210>  136
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Dab(GBE)

<400>  136

Gly Xaa Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr 
1               5                   10                  15      


Gln Leu Glu Asn Tyr Cys Asn 
            20              


<210>  137
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Dap(DHBA)

<400>  137

Gly Xaa Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr 
1               5                   10                  15      


Gln Leu Glu Asn Tyr Cys Asn 
            20              


<210>  138
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Dap(DHBA)

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Orn(DHBA)

<400>  138

Gly Xaa Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr 
1               5                   10                  15      


Gln Leu Glu Asn Tyr Cys Asn 
            20              


<210>  139
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is D-Orn(DHBA)

<400>  139

Gly Xaa Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr 
1               5                   10                  15      


Gln Leu Glu Asn Tyr Cys Asn 
            20              


<210>  140
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin peptide analog


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is dDap(alloc)

<400>  140

Gly Xaa Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr 
1               5                   10                  15      


Gln Leu Glu Asn Tyr Cys Asn 
            20              


