                               SEQUENCE LISTING

<110> TWIST BIOSCIENCE CORPORATION
 
<120> VARIANT NUCLEIC ACID LIBRARIES FOR GLYCANS

<130> 44854-826.601

<140> PCT/US2022/027330
<141> 2022-05-02

<150> 63/183,538
<151> 2021-05-03

<160> 6     

<170> PatentIn version 3.5

<210> 1
<211> 62
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (51)..(52)
<223> Thymidine-succinyl hexamide CED phosphoramidite

<400> 1
agacaatcaa ccatttgggg tggacagcct tgacctctag acttcggcat tttttttttt       60

tt                                                                      62


<210> 2
<211> 112
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide


<220>
<221> modified_base
<222> (101)..(102)
<223> Thymidine-succinyl hexamide CED phosphoramidite

<400> 2
cgggatcctt atcgtcatcg tcgtacagat cccgacccat ttgctgtcca ccagtcatgc       60

tagccatacc atgatgatga tgatgatgag aaccccgcat tttttttttt tt              112


<210> 3
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 3
atgcggggtt ctcatcatc                                                    19


<210> 4
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 4
cgggatcctt atcgtcatcg                                                   20


<210> 5
<211> 19
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (4)..(4)
<223> D, E, H, K, F, Q, M, G, or P

<220>
<221> MOD_RES
<222> (5)..(5)
<223> R, H, K, F, Y, T, M, I, L, or G

<220>
<221> MOD_RES
<222> (6)..(6)
<223> D, R, H, F, Y, W, S, N, A, I, or G

<220>
<221> MOD_RES
<222> (7)..(7)
<223> D, R, Y, I, L, S, T, N, A, V, G, or P

<220>
<221> MOD_RES
<222> (8)..(8)
<223> R, F, Y, S, T, A, V, G, or P

<220>
<221> MOD_RES
<222> (9)..(9)
<223> D, K, Y, W, S, T, N, A, V, L, G, or P

<220>
<221> MOD_RES
<222> (10)..(10)
<223> E, R, F, Y, W, S, M, A, or G

<220>
<221> MOD_RES
<222> (11)..(16)
<223> D, E, R, H, F, Y, W, T, N, V, P, A or absent

<220>
<221> SITE
<222> (11)..(16)
<223> This region may encompass 0-6 residues

<220>
<221> MOD_RES
<222> (17)..(17)
<223> D, E, S, N, A, or G

<220>
<221> MOD_RES
<222> (18)..(18)
<223> H, Y, W, S, A, V, I, L, or P

<400> 5
Cys Ala Arg Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
1               5                   10                  15      


Xaa Xaa Trp 
            


<210> 6
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xHis tag

<400> 6
His His His His His His 
1               5       


