                         SEQUENCE LISTING

<110>  Medigene Immunotherapies GmbH
 
<120>  Combination PRAME specific T cell receptors and chimeric 
       co-stimulatory receptor

<130>  M11754WO

<150>  EP21172722.7
<151>  2021-05-07

<160>  33    

<170>  PatentIn version 3.5

<210>  1
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  1

Ser Leu Leu Gln His Leu Ile Gly Leu 
1               5                   


<210>  2
<211>  5
<212>  PRT
<213>  homo sapiens

<400>  2

Ser Ile Phe Asn Thr 
1               5   


<210>  3
<211>  7
<212>  PRT
<213>  homo sapiens

<400>  3

Leu Tyr Lys Ala Gly Glu Leu 
1               5           


<210>  4
<211>  16
<212>  PRT
<213>  homo sapiens

<400>  4

Cys Ala Gly Leu Ala Asp Tyr Gly Gly Ser Gln Gly Asn Leu Ile Phe 
1               5                   10                  15      


<210>  5
<211>  5
<212>  PRT
<213>  homo sapiens

<400>  5

Ser Gly Asp Leu Ser 
1               5   


<210>  6
<211>  6
<212>  PRT
<213>  homo sapiens

<400>  6

Tyr Tyr Asn Gly Glu Glu 
1               5       


<210>  7
<211>  15
<212>  PRT
<213>  homo sapiens

<400>  7

Cys Ala Ser Ser Val Trp Ala Ser Gly Gly Tyr Glu Gln Tyr Phe 
1               5                   10                  15  


<210>  8
<211>  133
<212>  PRT
<213>  homo sapiens

<400>  8

Met Leu Leu Glu His Leu Leu Ile Ile Leu Trp Met Gln Leu Thr Trp 
1               5                   10                  15      


Val Ser Gly Gln Gln Leu Asn Gln Ser Pro Gln Ser Met Phe Ile Gln 
            20                  25                  30          


Glu Gly Glu Asp Val Ser Met Asn Cys Thr Ser Ser Ser Ile Phe Asn 
        35                  40                  45              


Thr Trp Leu Trp Tyr Lys Gln Asp Pro Gly Glu Gly Pro Val Leu Leu 
    50                  55                  60                  


Ile Ala Leu Tyr Lys Ala Gly Glu Leu Thr Ser Asn Gly Arg Leu Thr 
65                  70                  75                  80  


Ala Gln Phe Gly Ile Thr Arg Lys Asp Ser Phe Leu Asn Ile Ser Ala 
                85                  90                  95      


Ser Ile Pro Ser Asp Val Gly Ile Tyr Phe Cys Ala Gly Leu Ala Asp 
            100                 105                 110         


Tyr Gly Gly Ser Gln Gly Asn Leu Ile Phe Gly Lys Gly Thr Lys Leu 
        115                 120                 125             


Ser Val Lys Pro Asn 
    130             


<210>  9
<211>  133
<212>  PRT
<213>  homo sapiens

<400>  9

Met Gly Phe Arg Leu Leu Cys Cys Val Ala Phe Cys Leu Leu Gly Ala 
1               5                   10                  15      


Gly Pro Val Asp Ser Gly Val Thr Gln Thr Pro Lys His Leu Ile Thr 
            20                  25                  30          


Ala Thr Gly Gln Arg Val Thr Leu Arg Cys Ser Pro Arg Ser Gly Asp 
        35                  40                  45              


Leu Ser Val Tyr Trp Tyr Gln Gln Ser Leu Asp Gln Gly Leu Gln Phe 
    50                  55                  60                  


Leu Ile Gln Tyr Tyr Asn Gly Glu Glu Arg Ala Lys Gly Asn Ile Leu 
65                  70                  75                  80  


Glu Arg Phe Ser Ala Gln Gln Phe Pro Asp Leu His Ser Glu Leu Asn 
                85                  90                  95      


Leu Ser Ser Leu Glu Leu Gly Asp Ser Ala Leu Tyr Phe Cys Ala Ser 
            100                 105                 110         


Ser Val Trp Ala Ser Gly Gly Tyr Glu Gln Tyr Phe Gly Pro Gly Thr 
        115                 120                 125             


Arg Leu Thr Val Thr 
    130             


<210>  10
<211>  140
<212>  PRT
<213>  artificial

<220>
<223>  Ca

<400>  10

Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys Ser 
1               5                   10                  15      


Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr Asn 
            20                  25                  30          


Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Thr Val 
        35                  40                  45              


Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala Trp 
    50                  55                  60                  


Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser Ile 
65                  70                  75                  80  


Ile Pro Glu Asp Thr Phe Phe Pro Ser Ser Asp Val Pro Cys Asp Val 
                85                  90                  95      


Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr Asn Leu Asn Phe Gln 
            100                 105                 110         


Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu Leu Lys Val Ala Gly 
        115                 120                 125             


Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser 
    130                 135                 140 


<210>  11
<211>  179
<212>  PRT
<213>  artificial

<220>
<223>  Cb

<400>  11

Glu Asp Leu Lys Asn Val Phe Pro Pro Glu Val Ala Val Phe Glu Pro 
1               5                   10                  15      


Ser Lys Ala Glu Ile Ala His Thr Gln Lys Ala Thr Leu Val Cys Leu 
            20                  25                  30          


Ala Thr Gly Phe Tyr Pro Asp His Val Glu Leu Ser Trp Trp Val Asn 
        35                  40                  45              


Gly Lys Glu Val His Ser Gly Val Ser Thr Asp Pro Gln Pro Leu Lys 
    50                  55                  60                  


Glu Gln Pro Ala Leu Asn Asp Ser Arg Tyr Cys Leu Ser Ser Arg Leu 
65                  70                  75                  80  


Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His Phe Arg Cys 
                85                  90                  95      


Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp Thr Gln Asp 
            100                 105                 110         


Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala Trp Gly Arg 
        115                 120                 125             


Ala Asp Cys Gly Ile Thr Ser Arg Ser Tyr His Gln Gly Val Leu Ser 
    130                 135                 140                 


Ala Thr Ile Leu Tyr Glu Ile Leu Leu Gly Lys Ala Thr Leu Tyr Ala 
145                 150                 155                 160 


Val Leu Val Ser Ala Leu Val Leu Met Ala Met Val Lys Arg Lys Asp 
                165                 170                 175     


Ser Arg Gly 
            


<210>  12
<211>  15
<212>  DNA
<213>  artificial

<220>
<223>  CDR1_a

<400>  12
agcatattta acacc                                                        15


<210>  13
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  CDR2_a

<400>  13
ttatataagg ctggtgaatt g                                                 21


<210>  14
<211>  48
<212>  DNA
<213>  artificial

<220>
<223>  CDR3_a

<400>  14
tgtgccggcc tggccgatta cggcggctct cagggaaatc tgatcttc                    48


<210>  15
<211>  15
<212>  DNA
<213>  artificial

<220>
<223>  CDR1_b

<400>  15
tctggagacc tctct                                                        15


<210>  16
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  CDR2_b

<400>  16
tattataatg gagaagag                                                     18


<210>  17
<211>  45
<212>  DNA
<213>  artificial

<220>
<223>  CDR3_b

<400>  17
tgtgcctcta gcgtgtgggc ctctggcggc tacgagcagt atttt                       45


<210>  18
<211>  399
<212>  DNA
<213>  artificial

<220>
<223>  Va

<400>  18
atgctgctgg aacatctgct gatcatcctg tggatgcagc tgacctgggt ttccggccag       60

cagctgaatc agagccctca gagcatgttc atccaagaag gcgaggacgt ttccatgaat      120

tgcaccagca gcagcatctt caacacctgg ctgtggtaca agcaggaccc tggcgaagga      180

ccagtgctgc tgatcgcctt gtacaaagcc ggcgagctga ccagcaacgg cagactgaca      240

gcccagttcg gcattacccg gaaggacagc ttcctgaaca tctccgccag cattccctcc      300

gacgtgggca tctatttttg tgccggcctg gccgattacg gcggctctca gggaaatctg      360

atcttcggca agggcaccaa gctgagcgtg aagcccaac                             399


<210>  19
<211>  399
<212>  DNA
<213>  artificial

<220>
<223>  Vb

<400>  19
atgggcttca gactgctgtg ctgcgtggcc ttttgtctgc ttggagccgg acctgtggat       60

agcggcgtta cccagacacc taagcacctg atcacagcca caggccagcg cgtgaccctg      120

agatgttctc ctagaagcgg cgacctgagc gtgtactggt atcagcagtc tctggaccag      180

ggcctgcagt tcctgatcca gtactacaac ggcgaggaaa gagccaaggg caacatcctg      240

gaacggttca gcgcccagca gttcccagat ctgcacagcg agctgaacct gagcagcctg      300

gaactgggag atagcgccct gtacttctgt gcctctagcg tgtgggcctc tggcggctac      360

gagcagtatt ttggccctgg caccagactg accgtgacc                             399


<210>  20
<211>  420
<212>  DNA
<213>  artificial

<220>
<223>  Ca

<400>  20
attcagaacc ccgatcctgc cgtgtaccag ctgagagaca gcaagagcag cgacaagagc       60

gtgtgcctgt tcaccgactt cgacagccag accaacgtgt cccagagcaa ggacagcgac      120

gtgtacatca ccgacaagac cgtgctggac atgcggagca tggacttcaa gagcaacagc      180

gccgtggcct ggtccaacaa gagcgatttc gcctgcgcca acgccttcaa caacagcatt      240

atccccgagg acacattctt ccccagctcc gatgtgccct gcgacgtgaa gctggtggaa      300

aagagcttcg agacagacac caacctgaac ttccagaacc tgtccgtgat cggcttcaga      360

atcctgctgc tgaaggtggc cggcttcaac ctgctgatga cactgagact gtggtccagc      420


<210>  21
<211>  537
<212>  DNA
<213>  artificial

<220>
<223>  Cb

<400>  21
gaggatctga agaacgtgtt cccacctgag gtggccgtgt tcgagccttc taaggccgag       60

attgcccaca cacagaaagc cacactcgtg tgtctggcca ccggcttcta tcccgatcac      120

gtggaactgt cttggtgggt caacggcaaa gaggtgcaca gcggcgtcag cacagatccc      180

cagcctctga aagaacagcc cgctctgaac gacagccggt actgtctgag cagcagactg      240

agagtgtccg ccaccttctg gcagaacccc agaaaccact tcagatgcca ggtgcagttc      300

tacggcctga gcgagaacga tgagtggacc caggacagag ctaagcccgt gacacagatc      360

gtgtctgccg aagcttgggg cagagccgat tgtggcatca ccagcagatc ttaccaccag      420

ggcgtgctga gcgccaccat cctgtatgag atcctgctgg gcaaagccac tctgtacgcc      480

gtgctggtgt ctgccctggt gctgatggcc atggtcaagc ggaaggatag cagaggc         537


<210>  22
<211>  312
<212>  PRT
<213>  artificial

<220>
<223>  TCRb

<400>  22

Met Gly Phe Arg Leu Leu Cys Cys Val Ala Phe Cys Leu Leu Gly Ala 
1               5                   10                  15      


Gly Pro Val Asp Ser Gly Val Thr Gln Thr Pro Lys His Leu Ile Thr 
            20                  25                  30          


Ala Thr Gly Gln Arg Val Thr Leu Arg Cys Ser Pro Arg Ser Gly Asp 
        35                  40                  45              


Leu Ser Val Tyr Trp Tyr Gln Gln Ser Leu Asp Gln Gly Leu Gln Phe 
    50                  55                  60                  


Leu Ile Gln Tyr Tyr Asn Gly Glu Glu Arg Ala Lys Gly Asn Ile Leu 
65                  70                  75                  80  


Glu Arg Phe Ser Ala Gln Gln Phe Pro Asp Leu His Ser Glu Leu Asn 
                85                  90                  95      


Leu Ser Ser Leu Glu Leu Gly Asp Ser Ala Leu Tyr Phe Cys Ala Ser 
            100                 105                 110         


Ser Val Trp Ala Ser Gly Gly Tyr Glu Gln Tyr Phe Gly Pro Gly Thr 
        115                 120                 125             


Arg Leu Thr Val Thr Glu Asp Leu Lys Asn Val Phe Pro Pro Glu Val 
    130                 135                 140                 


Ala Val Phe Glu Pro Ser Lys Ala Glu Ile Ala His Thr Gln Lys Ala 
145                 150                 155                 160 


Thr Leu Val Cys Leu Ala Thr Gly Phe Tyr Pro Asp His Val Glu Leu 
                165                 170                 175     


Ser Trp Trp Val Asn Gly Lys Glu Val His Ser Gly Val Ser Thr Asp 
            180                 185                 190         


Pro Gln Pro Leu Lys Glu Gln Pro Ala Leu Asn Asp Ser Arg Tyr Cys 
        195                 200                 205             


Leu Ser Ser Arg Leu Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg 
    210                 215                 220                 


Asn His Phe Arg Cys Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp 
225                 230                 235                 240 


Glu Trp Thr Gln Asp Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala 
                245                 250                 255     


Glu Ala Trp Gly Arg Ala Asp Cys Gly Ile Thr Ser Arg Ser Tyr His 
            260                 265                 270         


Gln Gly Val Leu Ser Ala Thr Ile Leu Tyr Glu Ile Leu Leu Gly Lys 
        275                 280                 285             


Ala Thr Leu Tyr Ala Val Leu Val Ser Ala Leu Val Leu Met Ala Met 
    290                 295                 300                 


Val Lys Arg Lys Asp Ser Arg Gly 
305                 310         


<210>  23
<211>  936
<212>  DNA
<213>  artificial

<220>
<223>  TCRb

<400>  23
atgggcttca gactgctgtg ctgcgtggcc ttttgtctgc ttggagccgg acctgtggat       60

agcggcgtta cccagacacc taagcacctg atcacagcca caggccagcg cgtgaccctg      120

agatgttctc ctagaagcgg cgacctgagc gtgtactggt atcagcagtc tctggaccag      180

ggcctgcagt tcctgatcca gtactacaac ggcgaggaaa gagccaaggg caacatcctg      240

gaacggttca gcgcccagca gttcccagat ctgcacagcg agctgaacct gagcagcctg      300

gaactgggag atagcgccct gtacttctgt gcctctagcg tgtgggcctc tggcggctac      360

gagcagtatt ttggccctgg caccagactg accgtgaccg aggatctgaa gaacgtgttc      420

ccacctgagg tggccgtgtt cgagccttct aaggccgaga ttgcccacac acagaaagcc      480

acactcgtgt gtctggccac cggcttctat cccgatcacg tggaactgtc ttggtgggtc      540

aacggcaaag aggtgcacag cggcgtcagc acagatcccc agcctctgaa agaacagccc      600

gctctgaacg acagccggta ctgtctgagc agcagactga gagtgtccgc caccttctgg      660

cagaacccca gaaaccactt cagatgccag gtgcagttct acggcctgag cgagaacgat      720

gagtggaccc aggacagagc taagcccgtg acacagatcg tgtctgccga agcttggggc      780

agagccgatt gtggcatcac cagcagatct taccaccagg gcgtgctgag cgccaccatc      840

ctgtatgaga tcctgctggg caaagccact ctgtacgccg tgctggtgtc tgccctggtg      900

ctgatggcca tggtcaagcg gaaggatagc agaggc                                936


<210>  24
<211>  273
<212>  PRT
<213>  artificial

<220>
<223>  TCRa

<400>  24

Met Leu Leu Glu His Leu Leu Ile Ile Leu Trp Met Gln Leu Thr Trp 
1               5                   10                  15      


Val Ser Gly Gln Gln Leu Asn Gln Ser Pro Gln Ser Met Phe Ile Gln 
            20                  25                  30          


Glu Gly Glu Asp Val Ser Met Asn Cys Thr Ser Ser Ser Ile Phe Asn 
        35                  40                  45              


Thr Trp Leu Trp Tyr Lys Gln Asp Pro Gly Glu Gly Pro Val Leu Leu 
    50                  55                  60                  


Ile Ala Leu Tyr Lys Ala Gly Glu Leu Thr Ser Asn Gly Arg Leu Thr 
65                  70                  75                  80  


Ala Gln Phe Gly Ile Thr Arg Lys Asp Ser Phe Leu Asn Ile Ser Ala 
                85                  90                  95      


Ser Ile Pro Ser Asp Val Gly Ile Tyr Phe Cys Ala Gly Leu Ala Asp 
            100                 105                 110         


Tyr Gly Gly Ser Gln Gly Asn Leu Ile Phe Gly Lys Gly Thr Lys Leu 
        115                 120                 125             


Ser Val Lys Pro Asn Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu 
    130                 135                 140                 


Arg Asp Ser Lys Ser Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe 
145                 150                 155                 160 


Asp Ser Gln Thr Asn Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile 
                165                 170                 175     


Thr Asp Lys Thr Val Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn 
            180                 185                 190         


Ser Ala Val Ala Trp Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala 
        195                 200                 205             


Phe Asn Asn Ser Ile Ile Pro Glu Asp Thr Phe Phe Pro Ser Ser Asp 
    210                 215                 220                 


Val Pro Cys Asp Val Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr 
225                 230                 235                 240 


Asn Leu Asn Phe Gln Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu 
                245                 250                 255     


Leu Lys Val Ala Gly Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser 
            260                 265                 270         


Ser 
    


<210>  25
<211>  819
<212>  DNA
<213>  artificial

<220>
<223>  TCRa

<400>  25
atgctgctgg aacatctgct gatcatcctg tggatgcagc tgacctgggt ttccggccag       60

cagctgaatc agagccctca gagcatgttc atccaagaag gcgaggacgt ttccatgaat      120

tgcaccagca gcagcatctt caacacctgg ctgtggtaca agcaggaccc tggcgaagga      180

ccagtgctgc tgatcgcctt gtacaaagcc ggcgagctga ccagcaacgg cagactgaca      240

gcccagttcg gcattacccg gaaggacagc ttcctgaaca tctccgccag cattccctcc      300

gacgtgggca tctatttttg tgccggcctg gccgattacg gcggctctca gggaaatctg      360

atcttcggca agggcaccaa gctgagcgtg aagcccaaca ttcagaaccc cgatcctgcc      420

gtgtaccagc tgagagacag caagagcagc gacaagagcg tgtgcctgtt caccgacttc      480

gacagccaga ccaacgtgtc ccagagcaag gacagcgacg tgtacatcac cgacaagacc      540

gtgctggaca tgcggagcat ggacttcaag agcaacagcg ccgtggcctg gtccaacaag      600

agcgatttcg cctgcgccaa cgccttcaac aacagcatta tccccgagga cacattcttc      660

cccagctccg atgtgccctg cgacgtgaag ctggtggaaa agagcttcga gacagacacc      720

aacctgaact tccagaacct gtccgtgatc ggcttcagaa tcctgctgct gaaggtggcc      780

ggcttcaacc tgctgatgac actgagactg tggtccagc                             819


<210>  26
<211>  233
<212>  PRT
<213>  artificial

<220>
<223>  PD1-41BB

<400>  26

Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      


Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          


Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
        35                  40                  45              


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
    50                  55                  60                  


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
                85                  90                  95      


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
            100                 105                 110         


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             


Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
    130                 135                 140                 


Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 


Arg Pro Ala Gly Gln Phe Gln Thr Leu Val Val Gly Val Val Gly Gly 
                165                 170                 175     


Leu Leu Gly Ser Leu Val Leu Leu Val Trp Val Leu Ala Val Ile Lys 
            180                 185                 190         


Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg 
        195                 200                 205             


Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro 
    210                 215                 220                 


Glu Glu Glu Glu Gly Gly Cys Glu Leu 
225                 230             


<210>  27
<211>  699
<212>  DNA
<213>  artificial

<220>
<223>  PD1-41BB

<400>  27
atgcaaattc ctcaagctcc ttggcctgtc gtgtgggccg ttctgcaact tggatggcgg       60

cctggctggt tcctggactc tcctgacaga ccctggaatc ctccaacatt cagccccgct      120

ctgctggtgg ttaccgaggg cgataatgcc accttcacct gtagcttcag caacaccagc      180

gagagcttcg tgctgaactg gtacagaatg agccccagca accagaccga caagctggcc      240

gcctttcctg aggatagatc tcagcccggc caggactgcc ggttcagagt tacacagctg      300

cccaacggcc gggacttcca catgtctgtc gtccgggcca gaagaaacga cagcggcaca      360

tatctgtgcg gcgccatttc tctggcccct aaggctcaga tcaaagagag cctgagagcc      420

gagctgagag tgacagaaag acgggccgaa gtgcccacag ctcacccttc accttctcca      480

agacctgccg gccagttcca gacactggtc gtgggagttg ttggcggact gctgggatct      540

ctggtgctgc ttgtttgggt gctcgccgtg atcaagcggg gcagaaagaa gctgctgtac      600

atcttcaagc agcccttcat gcggcccgtg cagaccacac aagaggaaga tggctgctcc      660

tgcagattcc ccgaggaaga agaaggcggc tgcgaactc                             699


<210>  28
<211>  170
<212>  PRT
<213>  homo sapiens

<400>  28

Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      


Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          


Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
        35                  40                  45              


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
    50                  55                  60                  


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
                85                  90                  95      


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
            100                 105                 110         


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             


Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
    130                 135                 140                 


Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 


Arg Pro Ala Gly Gln Phe Gln Thr Leu Val 
                165                 170 


<210>  29
<211>  510
<212>  DNA
<213>  artificial

<220>
<223>  PD1 ECD

<400>  29
atgcaaattc ctcaagctcc ttggcctgtc gtgtgggccg ttctgcaact tggatggcgg       60

cctggctggt tcctggactc tcctgacaga ccctggaatc ctccaacatt cagccccgct      120

ctgctggtgg ttaccgaggg cgataatgcc accttcacct gtagcttcag caacaccagc      180

gagagcttcg tgctgaactg gtacagaatg agccccagca accagaccga caagctggcc      240

gcctttcctg aggatagatc tcagcccggc caggactgcc ggttcagagt tacacagctg      300

cccaacggcc gggacttcca catgtctgtc gtccgggcca gaagaaacga cagcggcaca      360

tatctgtgcg gcgccatttc tctggcccct aaggctcaga tcaaagagag cctgagagcc      420

gagctgagag tgacagaaag acgggccgaa gtgcccacag ctcacccttc accttctcca      480

agacctgccg gccagttcca gacactggtc                                       510


<210>  30
<211>  21
<212>  PRT
<213>  homo sapiens

<400>  30

Val Gly Val Val Gly Gly Leu Leu Gly Ser Leu Val Leu Leu Val Trp 
1               5                   10                  15      


Val Leu Ala Val Ile 
            20      


<210>  31
<211>  63
<212>  DNA
<213>  artificial

<220>
<223>  PD1 TM

<400>  31
gtgggagttg ttggcggact gctgggatct ctggtgctgc ttgtttgggt gctcgccgtg       60

atc                                                                     63


<210>  32
<211>  42
<212>  PRT
<213>  homo sapiens

<400>  32

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  33
<211>  126
<212>  DNA
<213>  artificial

<220>
<223>  41BB_ICD

<400>  33
aagcggggca gaaagaagct gctgtacatc ttcaagcagc ccttcatgcg gcccgtgcag       60

accacacaag aggaagatgg ctgctcctgc agattccccg aggaagaaga aggcggctgc      120

gaactc                                                                 126


