               SEQUENCE LISTING

<110> Medizinische Hochschule Hannover

<120> Glykoproteins for pseudotyping retroviral vector particles

<130> M1092PCT

<150> EP21172894.4
<151> 2021-05-07

<160> 65

<170> BiSSAP 1.3.6

<210> 1
<211> 509
<212> PRT
<213> Artificial Sequence


<220> 
<223> ectodomain

<400> 1
Met Lys Leu Pro Thr Gly Met Val Ile Leu Cys Ser Leu Ile Ile Val 
1               5                   10                  15      
Arg Ala Gly Phe Asp Asp Pro Arg Lys Ala Ile Ala Leu Val Gln Lys 
            20                  25                  30          
Gln His Gly Lys Pro Cys Glu Cys Ser Gly Gly Gln Val Ser Glu Ala 
        35                  40                  45              
Pro Pro Asn Ser Ile Gln Gln Val Thr Cys Pro Gly Lys Thr Ala Tyr 
    50                  55                  60                  
Leu Met Thr Asn Gln Lys Trp Lys Cys Arg Val Thr Pro Lys Asn Leu 
65                  70                  75                  80  
Thr Pro Ser Gly Gly Glu Leu Gln Asn Cys Pro Cys Asn Thr Phe Gln 
                85                  90                  95      
Asp Ser Met His Ser Ser Cys Tyr Thr Glu Tyr Arg Gln Cys Arg Ala 
            100                 105                 110         
Asn Asn Lys Thr Tyr Tyr Thr Ala Thr Leu Leu Lys Ile Arg Ser Gly 
        115                 120                 125             
Ser Leu Asn Glu Val Gln Ile Leu Gln Asn Pro Asn Gln Leu Leu Gln 
    130                 135                 140                 
Ser Pro Cys Arg Gly Ser Ile Asn Gln Pro Val Cys Trp Ser Ala Thr 
145                 150                 155                 160 
Ala Pro Ile His Ile Ser Asp Gly Gly Gly Pro Leu Asp Thr Lys Arg 
                165                 170                 175     
Val Trp Thr Val Gln Lys Arg Leu Glu Gln Ile His Lys Ala Met His 
            180                 185                 190         
Pro Glu Leu Gln Tyr His Pro Leu Ala Leu Pro Lys Val Arg Asp Asp 
        195                 200                 205             
Leu Ser Leu Asp Ala Arg Thr Phe Asp Ile Leu Asn Thr Thr Phe Arg 
    210                 215                 220                 
Leu Leu Gln Met Ser Asn Phe Ser Leu Ala Gln Asp Cys Trp Leu Cys 
225                 230                 235                 240 
Leu Lys Leu Gly Thr Pro Thr Pro Leu Ala Ile Pro Thr Pro Ser Leu 
                245                 250                 255     
Thr Tyr Ser Leu Ala Asp Ser Leu Ala Asn Ala Ser Cys Gln Ile Ile 
            260                 265                 270         
Pro Pro Leu Leu Val Gln Pro Met Gln Phe Ser Asn Ser Ser Cys Leu 
        275                 280                 285             
Ser Ser Pro Phe Ile Asn Asp Thr Glu Gln Ile Asp Leu Gly Ala Val 
    290                 295                 300                 
Thr Phe Thr Asn Cys Thr Ser Val Ala Asn Val Ser Ser Pro Leu Cys 
305                 310                 315                 320 
Ala Leu Asn Gly Ser Val Phe Leu Cys Gly Asn Asn Met Ala Tyr Thr 
                325                 330                 335     
Tyr Leu Pro Gln Asn Trp Thr Gly Leu Cys Val Gln Ala Ser Leu Leu 
            340                 345                 350         
Pro Asp Ile Asp Ile Ile Pro Gly Asp Glu Pro Val Pro Ile Pro Ala 
        355                 360                 365             
Ile Asp His Tyr Ile His Arg Pro Lys Arg Ala Val Gln Phe Ile Pro 
    370                 375                 380                 
Leu Leu Ala Gly Leu Gly Ile Thr Ala Ala Phe Thr Thr Gly Ala Thr 
385                 390                 395                 400 
Gly Leu Gly Val Ser Val Thr Gln Tyr Thr Lys Leu Ser His Gln Leu 
                405                 410                 415     
Ile Ser Asp Val Gln Val Leu Ser Gly Thr Ile Gln Asp Leu Gln Asp 
            420                 425                 430         
Gln Val Asp Ser Leu Ala Glu Val Val Leu Gln Asn Arg Arg Gly Leu 
        435                 440                 445             
Asp Leu Leu Thr Ala Glu Gln Gly Gly Ile Cys Leu Ala Leu Gln Glu 
    450                 455                 460                 
Lys Cys Cys Phe Tyr Ala Asn Lys Ser Gly Ile Val Arg Asn Lys Ile 
465                 470                 475                 480 
Arg Thr Leu Gln Glu Glu Leu Gln Lys Arg Arg Glu Ser Leu Ala Ser 
                485                 490                 495     
Asn Pro Leu Trp Thr Gly Leu Gln Gly Phe Leu Pro Tyr 
            500                 505                 

<210> 2
<211> 22
<212> PRT
<213> Artificial Sequence


<220> 
<223> transmembrane domain

<400> 2
Leu Leu Pro Leu Leu Gly Pro Leu Leu Thr Leu Leu Leu Ile Leu Thr 
1               5                   10                  15      
Ile Gly Pro Cys Val Phe 
            20          

<210> 3
<211> 34
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<223> CTT

<220> 
<221> CHAIN
<222> 14..21
<223> protease site

<220> 
<221> CHAIN
<222> 18..34
<223> R domain

<400> 3
Ser Arg Leu Met Ala Phe Ile Asn Asp Arg Leu Asn Val Val His Ala 
1               5                   10                  15      
Met Val Leu Ala Gln Gln Tyr Gln Ala Leu Lys Ala Glu Glu Glu Ala 
            20                  25                  30          
Gln Asp 
        

<210> 4
<211> 33
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<223> truncated CTT

<220> 
<221> CHAIN
<222> 18..33
<223> truncated R-domain

<400> 4
Ser Arg Leu Met Ala Phe Ile Asn Asp Arg Leu Asn Val Val His Ala 
1               5                   10                  15      
Met Val Leu Ala Gln Gln Tyr Gln Ala Leu Lys Ala Glu Glu Glu Ala 
            20                  25                  30          
Gln 
    

<210> 5
<211> 32
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<223> truncated CTT

<220> 
<221> CHAIN
<222> 18..32
<223> truncated R-domain

<400> 5
Ser Arg Leu Met Ala Phe Ile Asn Asp Arg Leu Asn Val Val His Ala 
1               5                   10                  15      
Met Val Leu Ala Gln Gln Tyr Gln Ala Leu Lys Ala Glu Glu Glu Ala 
            20                  25                  30          



<210> 6
<211> 31
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<223> truncated CTT

<220> 
<221> CHAIN
<222> 18..31
<223> truncated R-domain

<400> 6
Ser Arg Leu Met Ala Phe Ile Asn Asp Arg Leu Asn Val Val His Ala 
1               5                   10                  15      
Met Val Leu Ala Gln Gln Tyr Gln Ala Leu Lys Ala Glu Glu Glu 
            20                  25                  30      

<210> 7
<211> 30
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> R-domain

<220> 
<223> truncated CTT

<220> 
<221> CHAIN
<222> 18..30
<223> truncated R-domain

<400> 7
Ser Arg Leu Met Ala Phe Ile Asn Asp Arg Leu Asn Val Val His Ala 
1               5                   10                  15      
Met Val Leu Ala Gln Gln Tyr Gln Ala Leu Lys Ala Glu Glu 
            20                  25                  30  

<210> 8
<211> 29
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<223> truncated CTT

<220> 
<221> CHAIN
<222> 18..29
<223> truncated R-domain

<400> 8
Ser Arg Leu Met Ala Phe Ile Asn Asp Arg Leu Asn Val Val His Ala 
1               5                   10                  15      
Met Val Leu Ala Gln Gln Tyr Gln Ala Leu Lys Ala Glu 
            20                  25                  

<210> 9
<211> 28
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<223> truncated CTT

<220> 
<221> CHAIN
<222> 18..28
<223> truncated R-domain

<400> 9
Ser Arg Leu Met Ala Phe Ile Asn Asp Arg Leu Asn Val Val His Ala 
1               5                   10                  15      
Met Val Leu Ala Gln Gln Tyr Gln Ala Leu Lys Ala 
            20                  25              

<210> 10
<211> 27
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> R-domain

<220> 
<223> truncated CTT

<220> 
<221> CHAIN
<222> 18..27
<223> truncated R-domain

<400> 10
Ser Arg Leu Met Ala Phe Ile Asn Asp Arg Leu Asn Val Val His Ala 
1               5                   10                  15      
Met Val Leu Ala Gln Gln Tyr Gln Ala Leu Lys 
            20                  25          

<210> 11
<211> 26
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<223> truncated CTT

<220> 
<221> CHAIN
<222> 18..26
<223> truncated R-domain

<400> 11
Ser Arg Leu Met Ala Phe Ile Asn Asp Arg Leu Asn Val Val His Ala 
1               5                   10                  15      
Met Val Leu Ala Gln Gln Tyr Gln Ala Leu 
            20                  25      

<210> 12
<211> 25
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<223> truncated CTT

<220> 
<221> CHAIN
<222> 18..25
<223> truncated R-domain

<400> 12
Ser Arg Leu Met Ala Phe Ile Asn Asp Arg Leu Asn Val Val His Ala 
1               5                   10                  15      
Met Val Leu Ala Gln Gln Tyr Gln Ala 
            20                  25  

<210> 13
<211> 24
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<223> truncated CTT

<220> 
<221> CHAIN
<222> 18..24
<223> truncated R-domain

<400> 13
Ser Arg Leu Met Ala Phe Ile Asn Asp Arg Leu Asn Val Val His Ala 
1               5                   10                  15      
Met Val Leu Ala Gln Gln Tyr Gln 
            20                  

<210> 14
<211> 23
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<223> truncated CTT

<220> 
<221> CHAIN
<222> 18..23
<223> truncated R-domain

<400> 14
Ser Arg Leu Met Ala Phe Ile Asn Asp Arg Leu Asn Val Val His Ala 
1               5                   10                  15      
Met Val Leu Ala Gln Gln Tyr 
            20              

<210> 15
<211> 22
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<221> CHAIN
<222> 1..22
<223> truncated R-domain

<220> 
<223> truncated CTT

<400> 15
Ser Arg Leu Met Ala Phe Ile Asn Asp Arg Leu Asn Val Val His Ala 
1               5                   10                  15      
Met Val Leu Ala Gln Gln 
            20          

<210> 16
<211> 21
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<223> truncated CTT

<220> 
<221> CHAIN
<222> 18..21
<223> truncated R-domain

<400> 16
Ser Arg Leu Met Ala Phe Ile Asn Asp Arg Leu Asn Val Val His Ala 
1               5                   10                  15      
Met Val Leu Ala Gln 
            20      

<210> 17
<211> 20
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<223> truncated CTT

<220> 
<221> CHAIN
<222> 18..20
<223> truncated R-domain

<400> 17
Ser Arg Leu Met Ala Phe Ile Asn Asp Arg Leu Asn Val Val His Ala 
1               5                   10                  15      
Met Val Leu Ala 
            20  

<210> 18
<211> 19
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<223> truncated CTT

<220> 
<221> CHAIN
<222> 18..19
<223> truncated R-domain

<400> 18
Ser Arg Leu Met Ala Phe Ile Asn Asp Arg Leu Asn Val Val His Ala 
1               5                   10                  15      
Met Val Leu 
            

<210> 19
<211> 18
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<223> truncated CTT

<220> 
<221> CHAIN
<222> 18
<223> truncated R-domain

<400> 19
Ser Arg Leu Met Ala Phe Ile Asn Asp Arg Leu Asn Val Val His Ala 
1               5                   10                  15      
Met Val 
        

<210> 20
<211> 17
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<223> truncated CTT

<400> 20
Ser Arg Leu Met Ala Phe Ile Asn Asp Arg Leu Asn Val Val His Ala 
1               5                   10                  15      
Met 
    

<210> 21
<211> 506
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..506
<223> ectodomain

<220> 
<223> ectodomain

<400> 21
Met Gly Phe Thr Thr Lys Ile Ile Phe Leu Tyr Asn Leu Val Leu Val 
1               5                   10                  15      
Tyr Ala Gly Phe Asp Asp Pro Arg Lys Ala Ile Glu Leu Val Gln Lys 
            20                  25                  30          
Arg Tyr Gly Arg Pro Cys Asp Cys Ser Gly Gly Gln Val Ser Glu Pro 
        35                  40                  45              
Pro Ser Asp Arg Val Ser Gln Val Thr Cys Ser Gly Lys Thr Ala Tyr 
    50                  55                  60                  
Leu Met Pro Asp Gln Arg Trp Lys Cys Lys Ser Ile Pro Lys Asp Thr 
65                  70                  75                  80  
Ser Pro Ser Gly Pro Leu Gln Glu Cys Pro Cys Asn Ser Tyr Gln Ser 
                85                  90                  95      
Ser Val His Ser Ser Cys Tyr Thr Ser Tyr Gln Gln Cys Arg Ser Gly 
            100                 105                 110         
Asn Lys Thr Tyr Tyr Thr Ala Thr Leu Leu Lys Thr Gln Thr Gly Gly 
        115                 120                 125             
Thr Ser Asp Val Gln Val Leu Gly Ser Thr Asn Lys Leu Ile Gln Ser 
    130                 135                 140                 
Pro Cys Asn Gly Ile Lys Gly Gln Ser Ile Cys Trp Ser Thr Thr Ala 
145                 150                 155                 160 
Pro Ile His Val Ser Asp Gly Gly Gly Pro Leu Asp Thr Thr Arg Ile 
                165                 170                 175     
Lys Ser Val Gln Arg Lys Leu Glu Glu Ile His Lys Ala Leu Tyr Pro 
            180                 185                 190         
Glu Leu Gln Tyr His Pro Leu Ala Ile Pro Lys Val Arg Asp Asn Leu 
        195                 200                 205             
Met Val Asp Ala Gln Thr Leu Asn Ile Leu Asn Ala Thr Tyr Asn Leu 
    210                 215                 220                 
Leu Leu Met Ser Asn Thr Ser Leu Val Asp Asp Cys Trp Leu Cys Leu 
225                 230                 235                 240 
Lys Leu Gly Pro Pro Thr Pro Leu Ala Ile Pro Asn Phe Leu Leu Ser 
                245                 250                 255     
Tyr Val Thr Arg Ser Ser Asp Asn Ile Ser Cys Leu Ile Ile Pro Pro 
            260                 265                 270         
Leu Leu Val Gln Pro Met Gln Phe Ser Asn Ser Ser Cys Leu Phe Ser 
        275                 280                 285             
Pro Ser Tyr Asn Ser Thr Glu Glu Ile Asp Leu Gly His Val Ala Phe 
    290                 295                 300                 
Ser Asn Cys Thr Ser Ile Thr Asn Val Thr Gly Pro Ile Cys Ala Val 
305                 310                 315                 320 
Asn Gly Ser Val Phe Leu Cys Gly Asn Asn Met Ala Tyr Thr Tyr Leu 
                325                 330                 335     
Pro Thr Asn Trp Thr Gly Leu Cys Val Leu Ala Thr Leu Leu Pro Asp 
            340                 345                 350         
Ile Asp Ile Ile Pro Gly Asp Glu Pro Val Pro Ile Pro Ala Ile Asp 
        355                 360                 365             
His Phe Ile Tyr Arg Pro Lys Arg Ala Ile Gln Phe Ile Pro Leu Leu 
    370                 375                 380                 
Ala Gly Leu Gly Ile Thr Ala Ala Phe Thr Thr Gly Ala Thr Gly Leu 
385                 390                 395                 400 
Gly Val Ser Val Thr Gln Tyr Thr Lys Leu Ser Asn Gln Leu Ile Ser 
                405                 410                 415     
Asp Val Gln Ile Leu Ser Ser Thr Ile Gln Asp Leu Gln Asp Gln Val 
            420                 425                 430         
Asp Ser Leu Ala Glu Val Val Leu Gln Asn Arg Arg Gly Leu Asp Leu 
        435                 440                 445             
Leu Thr Ala Glu Gln Gly Gly Ile Cys Leu Ala Leu Gln Glu Lys Cys 
    450                 455                 460                 
Cys Phe Tyr Val Asn Lys Ser Gly Ile Val Arg Asp Lys Ile Lys Thr 
465                 470                 475                 480 
Leu Gln Glu Glu Leu Glu Arg Arg Arg Lys Asp Leu Ala Ser Asn Pro 
                485                 490                 495     
Leu Trp Thr Gly Leu Gln Gly Leu Leu Pro 
            500                 505     

<210> 22
<211> 23
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..23
<223> transmembrane domain

<220> 
<223> transmembrane domain

<400> 22
Tyr Leu Leu Pro Phe Leu Gly Pro Leu Leu Thr Leu Leu Leu Leu Leu 
1               5                   10                  15      
Thr Ile Gly Pro Cys Ile Phe 
            20              

<210> 23
<211> 34
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<223> CTT

<220> 
<221> CHAIN
<222> 18..34
<223> R domain

<400> 23
Asn Arg Leu Thr Ala Phe Ile Asn Asp Lys Leu Asn Ile Ile His Ala 
1               5                   10                  15      
Met Val Leu Thr Gln Gln Tyr Gln Val Leu Arg Thr Asp Glu Glu Ala 
            20                  25                  30          
Gln Asp 
        

<210> 24
<211> 33
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<223> truncated CTT

<220> 
<221> CHAIN
<222> 18..33
<223> truncated R-domain

<400> 24
Asn Arg Leu Thr Ala Phe Ile Asn Asp Lys Leu Asn Ile Ile His Ala 
1               5                   10                  15      
Met Val Leu Thr Gln Gln Tyr Gln Val Leu Arg Thr Asp Glu Glu Ala 
            20                  25                  30          
Gln 
    

<210> 25
<211> 32
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<223> truncated CTT

<220> 
<221> CHAIN
<222> 18..32
<223> truncated R-domain

<400> 25
Asn Arg Leu Thr Ala Phe Ile Asn Asp Lys Leu Asn Ile Ile His Ala 
1               5                   10                  15      
Met Val Leu Thr Gln Gln Tyr Gln Val Leu Arg Thr Asp Glu Glu Ala 
            20                  25                  30          



<210> 26
<211> 31
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<223> truncated CTT

<220> 
<221> CHAIN
<222> 18..31
<223> truncated R-domain

<400> 26
Asn Arg Leu Thr Ala Phe Ile Asn Asp Lys Leu Asn Ile Ile His Ala 
1               5                   10                  15      
Met Val Leu Thr Gln Gln Tyr Gln Val Leu Arg Thr Asp Glu Glu 
            20                  25                  30      

<210> 27
<211> 30
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<223> truncated CTT

<220> 
<221> CHAIN
<222> 18..30
<223> truncated R-domain

<400> 27
Asn Arg Leu Thr Ala Phe Ile Asn Asp Lys Leu Asn Ile Ile His Ala 
1               5                   10                  15      
Met Val Leu Thr Gln Gln Tyr Gln Val Leu Arg Thr Asp Glu 
            20                  25                  30  

<210> 28
<211> 29
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<223> truncated CTT

<220> 
<221> CHAIN
<222> 18..29
<223> truncated R-domain

<400> 28
Asn Arg Leu Thr Ala Phe Ile Asn Asp Lys Leu Asn Ile Ile His Ala 
1               5                   10                  15      
Met Val Leu Thr Gln Gln Tyr Gln Val Leu Arg Thr Asp 
            20                  25                  

<210> 29
<211> 28
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<223> truncated CTT

<220> 
<221> CHAIN
<222> 18..28
<223> truncated R-domain

<400> 29
Asn Arg Leu Thr Ala Phe Ile Asn Asp Lys Leu Asn Ile Ile His Ala 
1               5                   10                  15      
Met Val Leu Thr Gln Gln Tyr Gln Val Leu Arg Thr 
            20                  25              

<210> 30
<211> 27
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<223> truncated CTT

<220> 
<221> CHAIN
<222> 18..27
<223> truncated R-domain

<400> 30
Asn Arg Leu Thr Ala Phe Ile Asn Asp Lys Leu Asn Ile Ile His Ala 
1               5                   10                  15      
Met Val Leu Thr Gln Gln Tyr Gln Val Leu Arg 
            20                  25          

<210> 31
<211> 26
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<223> truncated CTT

<220> 
<221> CHAIN
<222> 18..26
<223> truncated R-domain

<400> 31
Asn Arg Leu Thr Ala Phe Ile Asn Asp Lys Leu Asn Ile Ile His Ala 
1               5                   10                  15      
Met Val Leu Thr Gln Gln Tyr Gln Val Leu 
            20                  25      

<210> 32
<211> 25
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<223> truncated CTT

<220> 
<221> CHAIN
<222> 18..25
<223> truncated R-domain

<400> 32
Asn Arg Leu Thr Ala Phe Ile Asn Asp Lys Leu Asn Ile Ile His Ala 
1               5                   10                  15      
Met Val Leu Thr Gln Gln Tyr Gln Val 
            20                  25  

<210> 33
<211> 24
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<223> truncated CTT

<220> 
<221> CHAIN
<222> 18..24
<223> truncated R-domain

<400> 33
Asn Arg Leu Thr Ala Phe Ile Asn Asp Lys Leu Asn Ile Ile His Ala 
1               5                   10                  15      
Met Val Leu Thr Gln Gln Tyr Gln 
            20                  

<210> 34
<211> 23
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<223> truncated CTT

<220> 
<221> CHAIN
<222> 18..23
<223> truncated R-domain

<400> 34
Asn Arg Leu Thr Ala Phe Ile Asn Asp Lys Leu Asn Ile Ile His Ala 
1               5                   10                  15      
Met Val Leu Thr Gln Gln Tyr 
            20              

<210> 35
<211> 22
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<223> truncated CTT

<220> 
<221> CHAIN
<222> 18..22
<223> truncated R-domain

<400> 35
Asn Arg Leu Thr Ala Phe Ile Asn Asp Lys Leu Asn Ile Ile His Ala 
1               5                   10                  15      
Met Val Leu Thr Gln Gln 
            20          

<210> 36
<211> 21
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<223> truncated CTT

<220> 
<221> CHAIN
<222> 18..21
<223> truncated R-domain

<400> 36
Asn Arg Leu Thr Ala Phe Ile Asn Asp Lys Leu Asn Ile Ile His Ala 
1               5                   10                  15      
Met Val Leu Thr Gln 
            20      

<210> 37
<211> 20
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<221> CHAIN
<222> 1..20
<223> truncated R-domain

<220> 
<223> truncated CTT

<400> 37
Asn Arg Leu Thr Ala Phe Ile Asn Asp Lys Leu Asn Ile Ile His Ala 
1               5                   10                  15      
Met Val Leu Thr 
            20  

<210> 38
<211> 19
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<223> truncated CTT

<220> 
<221> CHAIN
<222> 18..19
<223> truncated R-domain

<400> 38
Asn Arg Leu Thr Ala Phe Ile Asn Asp Lys Leu Asn Ile Ile His Ala 
1               5                   10                  15      
Met Val Leu 
            

<210> 39
<211> 18
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<221> CHAIN
<222> 1..17
<223> truncated R-domain

<220> 
<223> truncated CTT

<400> 39
Asn Arg Leu Thr Ala Phe Ile Asn Asp Lys Leu Asn Ile Ile His Ala 
1               5                   10                  15      
Met Val 
        

<210> 40
<211> 17
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..17
<223> T-domain

<220> 
<223> truncated CTT

<400> 40
Asn Arg Leu Thr Ala Phe Ile Asn Asp Lys Leu Asn Ile Ile His Ala 
1               5                   10                  15      
Met 
    

<210> 41
<211> 34
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..13
<223> RD114 T-domain (the 4 C-terminal amino acids forming the first
      half of the natural protease site are deleted)

<220> 
<223> artificial CTT

<220> 
<221> CHAIN
<222> 14..21
<223> ASLV-derived protease site

<220> 
<221> CHAIN
<222> 22..34
<223> RD114 R-domain (the 4 N-terminal amino acids forming the second
      half of the natural protease site are deleted)

<400> 41
Ser Arg Leu Met Ala Phe Ile Asn Asp Arg Leu Asn Val Val Val Ala 
1               5                   10                  15      
Met Pro Val Val Ile Gln Tyr Gln Ala Leu Lys Ala Glu Glu Glu Ala 
            20                  25                  30          
Gln Asp 
        

<210> 42
<211> 36
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..13
<223> RD114 T-domain (the 4 C-terminal amino acids forming the first
      half of the natural protease site are deleted)

<220> 
<223> artificial CTT

<220> 
<221> CHAIN
<222> 14..23
<223> synthetic protease site

<220> 
<221> CHAIN
<222> 24..36
<223> RD114 R-domain (the 4 N-terminal amino acids forming the second
      half of the natural protease site are deleted)

<400> 42
Ser Arg Leu Met Ala Phe Ile Asn Asp Arg Leu Asn Val Gly Ser Gly 
1               5                   10                  15      
Ile Phe Leu Glu Thr Ser Leu Gln Tyr Gln Ala Leu Lys Ala Glu Glu 
            20                  25                  30          
Glu Ala Gln Asp 
        35      

<210> 43
<211> 586
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..547
<223> MPMV ectodomain and membrane-spanning domain

<220> 
<223> glycoprotein based on MPMV

<220> 
<221> CHAIN
<222> 548..586
<223> CTT from MPMV (T-domain and R-domain)

<400> 43
Met Asn Phe Asn Tyr His Phe Ile Trp Ser Leu Val Ile Leu Ser Gln 
1               5                   10                  15      
Ile Ser Gln Val Gln Ala Gly Phe Gly Asp Pro Arg Glu Ala Leu Ala 
            20                  25                  30          
Glu Ile Gln Gln Lys His Gly Lys Pro Cys Asp Cys Ala Gly Gly Tyr 
        35                  40                  45              
Val Ser Ser Pro Pro Ile Asn Ser Leu Thr Thr Val Ser Cys Ser Thr 
    50                  55                  60                  
His Thr Ala Tyr Ser Val Thr Asn Ser Leu Lys Trp Gln Cys Val Ser 
65                  70                  75                  80  
Thr Pro Thr Thr Pro Ser Asn Thr His Ile Gly Ser Cys Pro Gly Glu 
                85                  90                  95      
Cys Asn Thr Ile Ser Tyr Asp Ser Val His Ala Ser Cys Tyr Asn His 
            100                 105                 110         
Tyr Gln Gln Cys Asn Ile Gly Asn Lys Thr Tyr Leu Thr Ala Thr Ile 
        115                 120                 125             
Thr Gly Asp Arg Thr Pro Ala Ile Gly Asp Gly Asn Val Pro Thr Val 
    130                 135                 140                 
Leu Gly Thr Ser His Asn Leu Ile Thr Ala Gly Cys Pro Asn Gly Lys 
145                 150                 155                 160 
Lys Gly Gln Val Val Cys Trp Asn Ser Arg Pro Ser Val His Ile Ser 
                165                 170                 175     
Asp Gly Gly Gly Pro Gln Asp Lys Ala Arg Asp Ile Ile Val Asn Lys 
            180                 185                 190         
Lys Phe Glu Glu Leu His Arg Ser Leu Phe Pro Glu Leu Ser Tyr His 
        195                 200                 205             
Pro Leu Ala Leu Pro Glu Ala Arg Gly Lys Glu Lys Ile Asp Ala His 
    210                 215                 220                 
Thr Leu Asp Leu Leu Ala Thr Val His Ser Leu Leu Asn Ala Ser Gln 
225                 230                 235                 240 
Pro Ser Leu Ala Glu Asp Cys Trp Leu Cys Leu Gln Ser Gly Asp Pro 
                245                 250                 255     
Val Pro Leu Ala Leu Pro Tyr Asn Asp Thr Leu Cys Ser Asn Phe Ala 
            260                 265                 270         
Cys Leu Ser Asn His Ser Cys Pro Leu Thr Pro Pro Phe Leu Val Gln 
        275                 280                 285             
Pro Phe Asn Phe Thr Asp Ser Asn Cys Leu Tyr Ala His Tyr Gln Asn 
    290                 295                 300                 
Asn Ser Phe Asp Ile Asp Val Gly Leu Ala Ser Phe Thr Asn Cys Ser 
305                 310                 315                 320 
Ser Tyr Tyr Asn Val Ser Thr Ala Ser Lys Pro Ser Asn Ser Leu Cys 
                325                 330                 335     
Ala Pro Asn Ser Ser Val Phe Val Cys Gly Asn Asn Lys Ala Tyr Thr 
            340                 345                 350         
Tyr Leu Pro Thr Asn Trp Thr Gly Ser Cys Val Leu Ala Thr Leu Leu 
        355                 360                 365             
Pro Asp Ile Asp Ile Ile Pro Gly Ser Glu Pro Val Pro Ile Pro Ala 
    370                 375                 380                 
Ile Asp His Phe Leu Gly Lys Ala Lys Arg Ala Ile Gln Leu Ile Pro 
385                 390                 395                 400 
Leu Phe Val Gly Leu Gly Ile Thr Thr Ala Val Ser Thr Gly Ala Ala 
                405                 410                 415     
Gly Leu Gly Val Ser Ile Thr Gln Tyr Thr Lys Leu Ser His Gln Leu 
            420                 425                 430         
Ile Ser Asp Val Gln Ala Ile Ser Ser Thr Ile Gln Asp Leu Gln Asp 
        435                 440                 445             
Gln Val Asp Ser Leu Ala Glu Val Val Leu Gln Asn Arg Arg Gly Leu 
    450                 455                 460                 
Asp Leu Leu Thr Ala Glu Gln Gly Gly Ile Cys Leu Ala Leu Gln Glu 
465                 470                 475                 480 
Lys Cys Cys Phe Tyr Ala Asn Lys Ser Gly Ile Val Arg Asp Lys Ile 
                485                 490                 495     
Lys Asn Leu Gln Asp Asp Leu Glu Arg Arg Arg Arg Gln Leu Ile Asp 
            500                 505                 510         
Asn Pro Phe Trp Thr Ser Phe His Gly Phe Leu Pro Tyr Val Met Pro 
        515                 520                 525             
Leu Leu Gly Pro Leu Leu Cys Leu Leu Leu Val Leu Ser Phe Gly Pro 
    530                 535                 540                 
Ile Ile Phe Asn Lys Leu Met Thr Phe Ile Lys His Gln Ile Glu Ser 
545                 550                 555                 560 
Ile Gln Ala Lys Pro Ile Gln Val His Tyr His Arg Leu Glu Gln Glu 
                565                 570                 575     
Asp Ser Gly Gly Ser Tyr Leu Thr Leu Thr 
            580                 585     

<210> 44
<211> 1767
<212> DNA
<213> Artificial Sequence


<220> 
<223> wild-type glycoprotein MPMV

<220> 
<221> misc_feature
<222> 1..6
<223> /note="additional nucleotides to form Kozak"

<400> 44
gccaccatga acttcaatta tcatttcatc tggagcttag tgatactatc tcaaatatct      60

caagttcaag ccggttttgg agatccgcgt gaagccctgg cagaaataca acaaaaacat     120

ggtaaacctt gtgactgtgc tggaggatat gtttcctccc caccgattaa ctctcttaca     180

actgtttctt gctctactca tactgcttat tcagtgacaa actccctaaa atggcagtgt     240

gtgtcaactc ccactacccc tagcaataca catataggaa gttgtcccgg tgaatgcaac     300

acgatctcat atgattctgt acatgcctct tgctataacc actatcaaca atgtaacatt     360

ggtaataaaa catatctcac tgccactata actggagata gaactcctgc cattggtgac     420

gggaatgtcc ctacagtact agggactagt cacaacctca ttacagcagg ctgtcccaat     480

ggtaaaaagg gccaagtggt ctgttggaat agccgacctt ctgttcatat atctgatgga     540

ggagggcctc aagataaggc ccgcgacatt atagtaaata aaaagtttga ggaattgcac     600

aggtcgctgt tcccagaact ttcttaccat cctctggcct tgcccgaagc ccgtggtaaa     660

gaaaaaattg acgcacacac tcttgatctc cttgccactg tacatagttt actcaatgct     720

tcccaaccca gtttagccga agattgctgg ctgtgcttac agtcaggaga tcccgttcct     780

cttgccctgc cctataatga tacactctgc tctaactttg cctgtttatc taatcactcc     840

tgccctttaa cccccccttt tttagtacag ccctttaact tcactgattc caattgcctt     900

tacgctcatt atcaaaacaa ctcatttgac atagatgtag gtctagctag ctttactaat     960

tgctctagct attataacgt ttctacagcc tccaaaccct ctaattccct atgcgcccca    1020

aacagctcgg tttttgtatg cggtaacaat aaggcataca cttatctacc cacaaattgg    1080

acgggaagtt gtgtacttgc tactcttttg cccgatatag acatcattcc aggtagtgag    1140

cctgtcccca ttccagctat tgatcatttt ttaggcaaag ccaaaagagc aatccaactt    1200

atccccctgt tcgtagggtt aggtataact actgcagtat ctactggggc tgctggtcta    1260

ggggtttcca tcactcaata tacaaaatta tctcatcaac taatatcaga tgttcaagct    1320

atttctagca ctatacaaga tctccaagat caggtagact ctctagcaga agtagtactg    1380

caaaacagaa gaggattaga tctacttaca gcagagcagg gaggtatctg cttagcctta    1440

caggaaaaat gttgtttcta cgccaataaa tctggaatcg tcagagacaa gattaaaaac    1500

ctacaagacg acttagaaag acgccgaaga caactgatcg acaacccatt ttggaccagt    1560

tttcatggat tcctccctta tgttatgccc ctattaggcc ctttgctttg cttattgctt    1620

gtgttatctt tcggtccaat tattttcaac aagcttatga cctttattaa acatcaaatt    1680

gagagcatcc aggccaaacc tatacaagtc cattatcatc gccttgaaca agaagacagt    1740

ggtggctcat atttgacctt aacatag                                        1767


<210> 45
<211> 1767
<212> DNA
<213> Artificial Sequence


<220> 
<223> codon-optimized glycoprotein based on MPMV

<220> 
<221> misc_feature
<222> 1..6
<223> /note="additional nucleotides to form Kozak"

<400> 45
gccaccatga acttcaacta ccacttcatc tggtccctcg tgatcctgag ccagatcagc      60

caggtgcagg ccggcttcgg cgatcctaga gaagccctgg ccgagatcca gcagaagcac     120

ggcaagccct gcgattgcgc tggcggctat gtgtccagcc cccccatcaa tagcctgacc     180

accgtgtcct gcagcaccca cacagcctac agcgtgacca acagcctgaa gtggcagtgc     240

gtgtccaccc ccaccacccc tagcaatacc cacatcggca gctgtcccgg cgagtgcaac     300

accatcagct acgactctgt gcacgccagc tgctacaacc actaccagca gtgcaacatc     360

ggcaacaaga cctacctgac cgccaccatc accggcgaca gaacacccgc catcggcgac     420

ggcaatgtgc ctacagtgct gggcaccagc cacaacctga tcaccgccgg ctgccccaac     480

ggcaagaaag gacaggtcgt gtgctggaac agccggccca gcgtgcacat ctctgatggc     540

ggaggccctc aggacaaggc ccgggacatc atcgtgaaca agaagttcga ggaactgcac     600

agaagcctgt tccccgagct gagctaccac cctctggccc tgcctgaggc cagaggcaaa     660

gagaagatcg acgcccacac cctggacctg ctggccacag tgcacagcct gctgaatgcc     720

agccagccct ctctggccga ggattgctgg ctgtgtctgc agagcggcga ccctgtgcca     780

ctggctctgc cctacaacga caccctgtgc agcaacttcg cctgcctgtc caaccacagc     840

tgccccctga cccctccatt cctggtgcag cccttcaact tcaccgacag caactgcctg     900

tacgcccact atcagaacaa cagcttcgac atcgacgtgg gcctggccag cttcaccaac     960

tgcagcagct actacaatgt gtccaccgcc agcaagccca gcaattccct gtgcgccccc    1020

aacagcagcg tgttcgtgtg cggcaacaac aaggcctaca cctacctgcc caccaactgg    1080

accggctctt gcgtgctggc taccctgctg cccgacatcg atatcatccc cggcagcgag    1140

cccgtgccca tccctgccat tgatcacttt ctgggcaagg ccaagcgggc catccagctg    1200

atccctctgt ttgtgggact gggcatcacc accgccgtgt ctacaggcgc tgctggcctg    1260

ggagtgtcca tcacccagta caccaagctg tcccaccagc tgattagcga cgtgcaggcc    1320

atcagcagca ccatccagga cctgcaggac caggtggaca gcctggcaga agtggtgctg    1380

cagaacagac ggggcctgga tctgctgaca gccgagcagg gcggaatttg cctggcactg    1440

caggaaaagt gctgcttcta cgccaacaag agcggcatcg tgcgggacaa gatcaagaat    1500

ctgcaggacg acctggaacg gcggaggcgg cagctgatcg acaacccttt ctggaccagc    1560

ttccacggct ttctgcctta cgtgatgccc ctgctgggcc ccctgctgtg cctgctgctg    1620

gtgctgtctt tcggccccat catcttcaac aagctgatga ccttcatcaa gcaccagatc    1680

gagagcatcc aggccaagcc catccaggtg cactaccaca gactggaaca ggaagatagc    1740

ggcggctctt acctgaccct gacctga                                        1767


<210> 46
<211> 580
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..547
<223> MPMV ectodomain and membrane-spanning domain

<220> 
<223> hybrid

<220> 
<221> CHAIN
<222> 548..564
<223> MLV-A T-domain

<220> 
<221> CHAIN
<222> 548..580
<223> CTT from MLV-A

<220> 
<221> CHAIN
<222> 565..580
<223> MLV-A R-domain

<400> 46
Met Asn Phe Asn Tyr His Phe Ile Trp Ser Leu Val Ile Leu Ser Gln 
1               5                   10                  15      
Ile Ser Gln Val Gln Ala Gly Phe Gly Asp Pro Arg Glu Ala Leu Ala 
            20                  25                  30          
Glu Ile Gln Gln Lys His Gly Lys Pro Cys Asp Cys Ala Gly Gly Tyr 
        35                  40                  45              
Val Ser Ser Pro Pro Ile Asn Ser Leu Thr Thr Val Ser Cys Ser Thr 
    50                  55                  60                  
His Thr Ala Tyr Ser Val Thr Asn Ser Leu Lys Trp Gln Cys Val Ser 
65                  70                  75                  80  
Thr Pro Thr Thr Pro Ser Asn Thr His Ile Gly Ser Cys Pro Gly Glu 
                85                  90                  95      
Cys Asn Thr Ile Ser Tyr Asp Ser Val His Ala Ser Cys Tyr Asn His 
            100                 105                 110         
Tyr Gln Gln Cys Asn Ile Gly Asn Lys Thr Tyr Leu Thr Ala Thr Ile 
        115                 120                 125             
Thr Gly Asp Arg Thr Pro Ala Ile Gly Asp Gly Asn Val Pro Thr Val 
    130                 135                 140                 
Leu Gly Thr Ser His Asn Leu Ile Thr Ala Gly Cys Pro Asn Gly Lys 
145                 150                 155                 160 
Lys Gly Gln Val Val Cys Trp Asn Ser Arg Pro Ser Val His Ile Ser 
                165                 170                 175     
Asp Gly Gly Gly Pro Gln Asp Lys Ala Arg Asp Ile Ile Val Asn Lys 
            180                 185                 190         
Lys Phe Glu Glu Leu His Arg Ser Leu Phe Pro Glu Leu Ser Tyr His 
        195                 200                 205             
Pro Leu Ala Leu Pro Glu Ala Arg Gly Lys Glu Lys Ile Asp Ala His 
    210                 215                 220                 
Thr Leu Asp Leu Leu Ala Thr Val His Ser Leu Leu Asn Ala Ser Gln 
225                 230                 235                 240 
Pro Ser Leu Ala Glu Asp Cys Trp Leu Cys Leu Gln Ser Gly Asp Pro 
                245                 250                 255     
Val Pro Leu Ala Leu Pro Tyr Asn Asp Thr Leu Cys Ser Asn Phe Ala 
            260                 265                 270         
Cys Leu Ser Asn His Ser Cys Pro Leu Thr Pro Pro Phe Leu Val Gln 
        275                 280                 285             
Pro Phe Asn Phe Thr Asp Ser Asn Cys Leu Tyr Ala His Tyr Gln Asn 
    290                 295                 300                 
Asn Ser Phe Asp Ile Asp Val Gly Leu Ala Ser Phe Thr Asn Cys Ser 
305                 310                 315                 320 
Ser Tyr Tyr Asn Val Ser Thr Ala Ser Lys Pro Ser Asn Ser Leu Cys 
                325                 330                 335     
Ala Pro Asn Ser Ser Val Phe Val Cys Gly Asn Asn Lys Ala Tyr Thr 
            340                 345                 350         
Tyr Leu Pro Thr Asn Trp Thr Gly Ser Cys Val Leu Ala Thr Leu Leu 
        355                 360                 365             
Pro Asp Ile Asp Ile Ile Pro Gly Ser Glu Pro Val Pro Ile Pro Ala 
    370                 375                 380                 
Ile Asp His Phe Leu Gly Lys Ala Lys Arg Ala Ile Gln Leu Ile Pro 
385                 390                 395                 400 
Leu Phe Val Gly Leu Gly Ile Thr Thr Ala Val Ser Thr Gly Ala Ala 
                405                 410                 415     
Gly Leu Gly Val Ser Ile Thr Gln Tyr Thr Lys Leu Ser His Gln Leu 
            420                 425                 430         
Ile Ser Asp Val Gln Ala Ile Ser Ser Thr Ile Gln Asp Leu Gln Asp 
        435                 440                 445             
Gln Val Asp Ser Leu Ala Glu Val Val Leu Gln Asn Arg Arg Gly Leu 
    450                 455                 460                 
Asp Leu Leu Thr Ala Glu Gln Gly Gly Ile Cys Leu Ala Leu Gln Glu 
465                 470                 475                 480 
Lys Cys Cys Phe Tyr Ala Asn Lys Ser Gly Ile Val Arg Asp Lys Ile 
                485                 490                 495     
Lys Asn Leu Gln Asp Asp Leu Glu Arg Arg Arg Arg Gln Leu Ile Asp 
            500                 505                 510         
Asn Pro Phe Trp Thr Ser Phe His Gly Phe Leu Pro Tyr Val Met Pro 
        515                 520                 525             
Leu Leu Gly Pro Leu Leu Cys Leu Leu Leu Val Leu Ser Phe Gly Pro 
    530                 535                 540                 
Ile Ile Phe Asn Arg Leu Val Gln Phe Val Lys Asp Arg Ile Ser Val 
545                 550                 555                 560 
Val Gln Ala Leu Val Leu Thr Gln Gln Tyr His Gln Leu Lys Pro Leu 
                565                 570                 575     
Glu Tyr Glu Pro 
            580 

<210> 47
<211> 1749
<212> DNA
<213> Artificial Sequence


<220> 
<223> wild-type nucleic acid sequence of hybrid

<220> 
<221> misc_feature
<222> 1..6
<223> /note="additional nucleotides to form Kozak"

<400> 47
gccaccatga acttcaatta tcatttcatc tggagcttag tgatactatc tcaaatatct      60

caagttcaag ccggttttgg agatccgcgt gaagccctgg cagaaataca acaaaaacat     120

ggtaaacctt gtgactgtgc tggaggatat gtttcctccc caccgattaa ctctcttaca     180

actgtttctt gctctactca tactgcttat tcagtgacaa actccctaaa atggcagtgt     240

gtgtcaactc ccactacccc tagcaataca catataggaa gttgtcccgg tgaatgcaac     300

acgatctcat atgattctgt acatgcctct tgctataacc actatcaaca atgtaacatt     360

ggtaataaaa catatctcac tgccactata actggagata gaactcctgc cattggtgac     420

gggaatgtcc ctacagtact agggactagt cacaacctca ttacagcagg ctgtcccaat     480

ggtaaaaagg gccaagtggt ctgttggaat agccgacctt ctgttcatat atctgatgga     540

ggagggcctc aagataaggc ccgcgacatt atagtaaata aaaagtttga ggaattgcac     600

aggtcgctgt tcccagaact ttcttaccat cctctggcct tgcccgaagc ccgtggtaaa     660

gaaaaaattg acgcacacac tcttgatctc cttgccactg tacatagttt actcaatgct     720

tcccaaccca gtttagccga agattgctgg ctgtgcttac agtcaggaga tcccgttcct     780

cttgccctgc cctataatga tacactctgc tctaactttg cctgtttatc taatcactcc     840

tgccctttaa cccccccttt tttagtacag ccctttaact tcactgattc caattgcctt     900

tacgctcatt atcaaaacaa ctcatttgac atagatgtag gtctagctag ctttactaat     960

tgctctagct attataacgt ttctacagcc tccaaaccct ctaattccct atgcgcccca    1020

aacagctcgg tttttgtatg cggtaacaat aaggcataca cttatctacc cacaaattgg    1080

acgggaagtt gtgtacttgc tactcttttg cccgatatag acatcattcc aggtagtgag    1140

cctgtcccca ttccagctat tgatcatttt ttaggcaaag ccaaaagagc aatccaactt    1200

atccccctgt tcgtagggtt aggtataact actgcagtat ctactggggc tgctggtcta    1260

ggggtttcca tcactcaata tacaaaatta tctcatcaac taatatcaga tgttcaagct    1320

atttctagca ctatacaaga tctccaagat caggtagact ctctagcaga agtagtactg    1380

caaaacagaa gaggattaga tctacttaca gcagagcagg gaggtatctg cttagcctta    1440

caggaaaaat gttgtttcta cgccaataaa tctggaatcg tcagagacaa gattaaaaac    1500

ctacaagacg acttagaaag acgccgaaga caactgatcg acaacccatt ttggaccagt    1560

tttcatggat tcctccctta tgttatgccc ctattaggcc ctttgctttg cttattgctt    1620

gtgttatctt tcggccccat catcttcaat cgattagttc aatttgttaa agacaggatc    1680

tcagtagtcc aggctttagt cctgactcaa caataccacc agctaaaacc actagaatac    1740

gagccgtga                                                            1749


<210> 48
<211> 1749
<212> DNA
<213> Artificial Sequence


<220> 
<223> codon-optimized nucleic acid sequence of hybrid

<220> 
<221> misc_feature
<222> 1..6
<223> /note="additional nucleotides to form Kozak"

<400> 48
gccaccatga acttcaacta ccacttcatc tggtccctcg tgatcctgag ccagatcagc      60

caggtgcagg ccggcttcgg cgatcctaga gaagccctgg ccgagatcca gcagaagcac     120

ggcaagccct gcgattgcgc tggcggctat gtgtccagcc cccccatcaa tagcctgacc     180

accgtgtcct gcagcaccca cacagcctac agcgtgacca acagcctgaa gtggcagtgc     240

gtgtccaccc ccaccacccc tagcaatacc cacatcggca gctgtcccgg cgagtgcaac     300

accatcagct acgactctgt gcacgccagc tgctacaacc actaccagca gtgcaacatc     360

ggcaacaaga cctacctgac cgccaccatc accggcgaca gaacacccgc catcggcgac     420

ggcaatgtgc ctacagtgct gggcaccagc cacaacctga tcaccgccgg ctgccccaac     480

ggcaagaaag gacaggtcgt gtgctggaac agccggccca gcgtgcacat ctctgatggc     540

ggaggccctc aggacaaggc ccgggacatc atcgtgaaca agaagttcga ggaactgcac     600

agaagcctgt tccccgagct gagctaccac cctctggccc tgcctgaggc cagaggcaaa     660

gagaagatcg acgcccacac cctggacctg ctggccacag tgcacagcct gctgaatgcc     720

agccagccct ctctggccga ggattgctgg ctgtgtctgc agagcggcga ccctgtgcca     780

ctggctctgc cctacaacga caccctgtgc agcaacttcg cctgcctgtc caaccacagc     840

tgccccctga cccctccatt cctggtgcag cccttcaact tcaccgacag caactgcctg     900

tacgcccact atcagaacaa cagcttcgac atcgacgtgg gcctggccag cttcaccaac     960

tgcagcagct actacaatgt gtccaccgcc agcaagccca gcaattccct gtgcgccccc    1020

aacagcagcg tgttcgtgtg cggcaacaac aaggcctaca cctacctgcc caccaactgg    1080

accggctctt gcgtgctggc taccctgctg cccgacatcg atatcatccc cggcagcgag    1140

cccgtgccca tccctgccat tgatcacttt ctgggcaagg ccaagcgggc catccagctg    1200

atccctctgt ttgtgggact gggcatcacc accgccgtgt ctacaggcgc tgctggcctg    1260

ggagtgtcca tcacccagta caccaagctg tcccaccagc tgattagcga cgtgcaggcc    1320

atcagcagca ccatccagga cctgcaggac caggtggaca gcctggcaga agtggtgctg    1380

cagaacagac ggggcctgga tctgctgaca gccgagcagg gcggaatttg cctggcactg    1440

caggaaaagt gctgcttcta cgccaacaag agcggcatcg tgcgggacaa gatcaagaat    1500

ctgcaggacg acctggaacg gcggaggcgg cagctgatcg acaacccttt ctggaccagc    1560

ttccacggct ttctgcctta cgtgatgccc ctgctgggcc ccctgctgtg cctgctgctg    1620

gtgctgtctt tcggccccat catcttcaac agactggtgc agttcgtgaa agaccggatc    1680

agcgtggtgc aggctctggt gctgacccag cagtaccatc agctgaagcc cctggaatac    1740

gagccctga                                                            1749


<210> 49
<211> 548
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..531
<223> RD114 ectodomain and membrane-spanning domain

<220> 
<223> hybrid

<220> 
<221> CHAIN
<222> 532..548
<223> MLV-A T-domain

<400> 49
Met Lys Leu Pro Thr Gly Met Val Ile Leu Cys Ser Leu Ile Ile Val 
1               5                   10                  15      
Arg Ala Gly Phe Asp Asp Pro Arg Lys Ala Ile Ala Leu Val Gln Lys 
            20                  25                  30          
Gln His Gly Lys Pro Cys Glu Cys Ser Gly Gly Gln Val Ser Glu Ala 
        35                  40                  45              
Pro Pro Asn Ser Ile Gln Gln Val Thr Cys Pro Gly Lys Thr Ala Tyr 
    50                  55                  60                  
Leu Met Thr Asn Gln Lys Trp Lys Cys Arg Val Thr Pro Lys Asn Leu 
65                  70                  75                  80  
Thr Pro Ser Gly Gly Glu Leu Gln Asn Cys Pro Cys Asn Thr Phe Gln 
                85                  90                  95      
Asp Ser Met His Ser Ser Cys Tyr Thr Glu Tyr Arg Gln Cys Arg Ala 
            100                 105                 110         
Asn Asn Lys Thr Tyr Tyr Thr Ala Thr Leu Leu Lys Ile Arg Ser Gly 
        115                 120                 125             
Ser Leu Asn Glu Val Gln Ile Leu Gln Asn Pro Asn Gln Leu Leu Gln 
    130                 135                 140                 
Ser Pro Cys Arg Gly Ser Ile Asn Gln Pro Val Cys Trp Ser Ala Thr 
145                 150                 155                 160 
Ala Pro Ile His Ile Ser Asp Gly Gly Gly Pro Leu Asp Thr Lys Arg 
                165                 170                 175     
Val Trp Thr Val Gln Lys Arg Leu Glu Gln Ile His Lys Ala Met His 
            180                 185                 190         
Pro Glu Leu Gln Tyr His Pro Leu Ala Leu Pro Lys Val Arg Asp Asp 
        195                 200                 205             
Leu Ser Leu Asp Ala Arg Thr Phe Asp Ile Leu Asn Thr Thr Phe Arg 
    210                 215                 220                 
Leu Leu Gln Met Ser Asn Phe Ser Leu Ala Gln Asp Cys Trp Leu Cys 
225                 230                 235                 240 
Leu Lys Leu Gly Thr Pro Thr Pro Leu Ala Ile Pro Thr Pro Ser Leu 
                245                 250                 255     
Thr Tyr Ser Leu Ala Asp Ser Leu Ala Asn Ala Ser Cys Gln Ile Ile 
            260                 265                 270         
Pro Pro Leu Leu Val Gln Pro Met Gln Phe Ser Asn Ser Ser Cys Leu 
        275                 280                 285             
Ser Ser Pro Phe Ile Asn Asp Thr Glu Gln Ile Asp Leu Gly Ala Val 
    290                 295                 300                 
Thr Phe Thr Asn Cys Thr Ser Val Ala Asn Val Ser Ser Pro Leu Cys 
305                 310                 315                 320 
Ala Leu Asn Gly Ser Val Phe Leu Cys Gly Asn Asn Met Ala Tyr Thr 
                325                 330                 335     
Tyr Leu Pro Gln Asn Trp Thr Gly Leu Cys Val Gln Ala Ser Leu Leu 
            340                 345                 350         
Pro Asp Ile Asp Ile Ile Pro Gly Asp Glu Pro Val Pro Ile Pro Ala 
        355                 360                 365             
Ile Asp His Tyr Ile His Arg Pro Lys Arg Ala Val Gln Phe Ile Pro 
    370                 375                 380                 
Leu Leu Ala Gly Leu Gly Ile Thr Ala Ala Phe Thr Thr Gly Ala Thr 
385                 390                 395                 400 
Gly Leu Gly Val Ser Val Thr Gln Tyr Thr Lys Leu Ser His Gln Leu 
                405                 410                 415     
Ile Ser Asp Val Gln Val Leu Ser Gly Thr Ile Gln Asp Leu Gln Asp 
            420                 425                 430         
Gln Val Asp Ser Leu Ala Glu Val Val Leu Gln Asn Arg Arg Gly Leu 
        435                 440                 445             
Asp Leu Leu Thr Ala Glu Gln Gly Gly Ile Cys Leu Ala Leu Gln Glu 
    450                 455                 460                 
Lys Cys Cys Phe Tyr Ala Asn Lys Ser Gly Ile Val Arg Asn Lys Ile 
465                 470                 475                 480 
Arg Thr Leu Gln Glu Glu Leu Gln Lys Arg Arg Glu Ser Leu Ala Ser 
                485                 490                 495     
Asn Pro Leu Trp Thr Gly Leu Gln Gly Phe Leu Pro Tyr Leu Leu Pro 
            500                 505                 510         
Leu Leu Gly Pro Leu Leu Thr Leu Leu Leu Ile Leu Thr Ile Gly Pro 
        515                 520                 525             
Cys Val Phe Asn Arg Leu Val Gln Phe Val Lys Asp Arg Ile Ser Val 
    530                 535                 540                 
Val Gln Ala Leu 
545             

<210> 50
<211> 1653
<212> DNA
<213> Artificial Sequence


<220> 
<223> codon-optimized hybrid

<220> 
<221> misc_feature
<222> 1..6
<223> /note="additional nucleotides to form Kozak"

<400> 50
gccaccatga aactcccaac aggaatggtc attttatgta gcctaataat agttcgggca      60

gggtttgacg acccccgcaa ggctatcgca ttagtacaaa aacaacatgg taaaccatgc     120

gaatgcagcg gagggcaggt atccgaggcc ccaccgaact ccatccaaca ggtaacttgc     180

ccaggcaaga cggcctactt aatgaccaac caaaaatgga aatgcagagt cactccaaaa     240

aatctcaccc ctagcggggg agaactccag aactgcccct gtaacacttt ccaggactcg     300

atgcacagtt cttgttatac tgaataccgg caatgcaggg cgaataataa gacatactac     360

acggccacct tgcttaaaat acggtctggg agcctcaacg aggtacagat attacaaaac     420

cccaatcagc tcctacagtc cccttgtagg ggctctataa atcagcccgt ttgctggagt     480

gccacagccc ccatccatat ctccgatggt ggaggacccc tcgatactaa gagagtgtgg     540

acagtccaaa aaaggctaga acaaattcat aaggctatgc atcctgaact tcaataccac     600

cccttagccc tgcccaaagt cagagatgac cttagccttg atgcacggac ttttgatatc     660

ctgaatacca cttttaggtt actccagatg tccaatttta gccttgccca agattgttgg     720

ctctgtttaa aactaggtac ccctacccct cttgcgatac ccactccctc tttaacctac     780

tccctagcag actccctagc gaatgcctcc tgtcagatta tacctcccct cttggttcaa     840

ccgatgcagt tctccaactc gtcctgttta tcttcccctt tcattaacga tacggaacaa     900

atagacttag gtgcagtcac ctttactaac tgcacctctg tagccaatgt cagtagtcct     960

ttatgtgccc taaacgggtc agtcttcctc tgtggaaata acatggcata cacctattta    1020

ccccaaaact ggacaggact ttgcgtccaa gcctccctcc tccccgacat tgacatcatc    1080

ccgggggatg agccagtccc cattcctgcc attgatcatt atatacatag acctaaacga    1140

gctgtacagt tcatcccttt actagctgga ctgggaatca ccgcagcatt caccaccgga    1200

gctacaggcc taggtgtctc cgtcacccag tatacaaaat tatcccatca gttaatatct    1260

gatgtccaag tcttatccgg taccatacaa gatttacaag accaggtaga ctcgttagct    1320

gaagtagttc tccaaaatag gaggggactg gacctactaa cggcagaaca aggaggaatt    1380

tgtttagcct tacaagaaaa atgctgtttt tatgctaaca agtcaggaat tgtgagaaac    1440

aaaataagaa ccctacaaga agaattacaa aaacgcaggg aaagcctggc atccaaccct    1500

ctctggaccg ggctgcaggg ctttcttccg tacctcctac ctctcctggg acccctactc    1560

accctcctac tcatactaac cattgggcca tgcgttttca acagactggt gcagttcgtg    1620

aaagaccgga tcagcgtggt gcaggctctg tga                                 1653


<210> 51
<211> 570
<212> PRT
<213> Artificial Sequence


<220> 
<221> CHAIN
<222> 1..531
<223> RD114 ectodomain and membrane-spanning domain

<220> 
<223> hybrid

<220> 
<221> CHAIN
<222> 532..570
<223> MPMV CTT (T-domain and R-domain)

<400> 51
Met Lys Leu Pro Thr Gly Met Val Ile Leu Cys Ser Leu Ile Ile Val 
1               5                   10                  15      
Arg Ala Gly Phe Asp Asp Pro Arg Lys Ala Ile Ala Leu Val Gln Lys 
            20                  25                  30          
Gln His Gly Lys Pro Cys Glu Cys Ser Gly Gly Gln Val Ser Glu Ala 
        35                  40                  45              
Pro Pro Asn Ser Ile Gln Gln Val Thr Cys Pro Gly Lys Thr Ala Tyr 
    50                  55                  60                  
Leu Met Thr Asn Gln Lys Trp Lys Cys Arg Val Thr Pro Lys Asn Leu 
65                  70                  75                  80  
Thr Pro Ser Gly Gly Glu Leu Gln Asn Cys Pro Cys Asn Thr Phe Gln 
                85                  90                  95      
Asp Ser Met His Ser Ser Cys Tyr Thr Glu Tyr Arg Gln Cys Arg Ala 
            100                 105                 110         
Asn Asn Lys Thr Tyr Tyr Thr Ala Thr Leu Leu Lys Ile Arg Ser Gly 
        115                 120                 125             
Ser Leu Asn Glu Val Gln Ile Leu Gln Asn Pro Asn Gln Leu Leu Gln 
    130                 135                 140                 
Ser Pro Cys Arg Gly Ser Ile Asn Gln Pro Val Cys Trp Ser Ala Thr 
145                 150                 155                 160 
Ala Pro Ile His Ile Ser Asp Gly Gly Gly Pro Leu Asp Thr Lys Arg 
                165                 170                 175     
Val Trp Thr Val Gln Lys Arg Leu Glu Gln Ile His Lys Ala Met His 
            180                 185                 190         
Pro Glu Leu Gln Tyr His Pro Leu Ala Leu Pro Lys Val Arg Asp Asp 
        195                 200                 205             
Leu Ser Leu Asp Ala Arg Thr Phe Asp Ile Leu Asn Thr Thr Phe Arg 
    210                 215                 220                 
Leu Leu Gln Met Ser Asn Phe Ser Leu Ala Gln Asp Cys Trp Leu Cys 
225                 230                 235                 240 
Leu Lys Leu Gly Thr Pro Thr Pro Leu Ala Ile Pro Thr Pro Ser Leu 
                245                 250                 255     
Thr Tyr Ser Leu Ala Asp Ser Leu Ala Asn Ala Ser Cys Gln Ile Ile 
            260                 265                 270         
Pro Pro Leu Leu Val Gln Pro Met Gln Phe Ser Asn Ser Ser Cys Leu 
        275                 280                 285             
Ser Ser Pro Phe Ile Asn Asp Thr Glu Gln Ile Asp Leu Gly Ala Val 
    290                 295                 300                 
Thr Phe Thr Asn Cys Thr Ser Val Ala Asn Val Ser Ser Pro Leu Cys 
305                 310                 315                 320 
Ala Leu Asn Gly Ser Val Phe Leu Cys Gly Asn Asn Met Ala Tyr Thr 
                325                 330                 335     
Tyr Leu Pro Gln Asn Trp Thr Gly Leu Cys Val Gln Ala Ser Leu Leu 
            340                 345                 350         
Pro Asp Ile Asp Ile Ile Pro Gly Asp Glu Pro Val Pro Ile Pro Ala 
        355                 360                 365             
Ile Asp His Tyr Ile His Arg Pro Lys Arg Ala Val Gln Phe Ile Pro 
    370                 375                 380                 
Leu Leu Ala Gly Leu Gly Ile Thr Ala Ala Phe Thr Thr Gly Ala Thr 
385                 390                 395                 400 
Gly Leu Gly Val Ser Val Thr Gln Tyr Thr Lys Leu Ser His Gln Leu 
                405                 410                 415     
Ile Ser Asp Val Gln Val Leu Ser Gly Thr Ile Gln Asp Leu Gln Asp 
            420                 425                 430         
Gln Val Asp Ser Leu Ala Glu Val Val Leu Gln Asn Arg Arg Gly Leu 
        435                 440                 445             
Asp Leu Leu Thr Ala Glu Gln Gly Gly Ile Cys Leu Ala Leu Gln Glu 
    450                 455                 460                 
Lys Cys Cys Phe Tyr Ala Asn Lys Ser Gly Ile Val Arg Asn Lys Ile 
465                 470                 475                 480 
Arg Thr Leu Gln Glu Glu Leu Gln Lys Arg Arg Glu Ser Leu Ala Ser 
                485                 490                 495     
Asn Pro Leu Trp Thr Gly Leu Gln Gly Phe Leu Pro Tyr Leu Leu Pro 
            500                 505                 510         
Leu Leu Gly Pro Leu Leu Thr Leu Leu Leu Ile Leu Thr Ile Gly Pro 
        515                 520                 525             
Cys Val Phe Asn Lys Leu Met Thr Phe Ile Lys His Gln Ile Glu Ser 
    530                 535                 540                 
Ile Gln Ala Lys Pro Ile Gln Val His Tyr His Arg Leu Glu Gln Glu 
545                 550                 555                 560 
Asp Ser Gly Gly Ser Tyr Leu Thr Leu Thr 
                565                 570 

<210> 52
<211> 1719
<212> DNA
<213> Artificial Sequence


<220> 
<223> codon-optimized hybrid

<220> 
<221> misc_feature
<222> 1..6
<223> /note="additional nucleotides to form Kozak"

<400> 52
gccaccatga agctgcccac cggcatggtc atcctgtgca gcctgatcat cgtgcgggct      60

ggcttcgacg accccagaaa ggccattgcc ctggtgcaga agcagcacgg caagccctgc     120

gagtgttctg gcggacaggt gtccgaggcc cctcccaatt ctatccagca agtgacctgc     180

cccggcaaga ccgcctacct gatgaccaac cagaaatgga agtgcagagt gacccccaag     240

aacctgaccc cctctggcgg cgagctgcag aactgcccct gcaacacctt ccaggacagc     300

atgcactcca gctgctacac cgagtaccgg cagtgccggg ccaacaacaa gacctactac     360

accgccaccc tgctgaagat cagaagcggc agcctgaacg aggtgcagat cctgcagaac     420

cccaaccagc tgctgcagag cccttgcaga ggcagcatca accagcccgt gtgttggagc     480

gccaccgccc ctatccacat ctctgatggc ggcggacccc tggacaccaa gcgcgtgtgg     540

acagtgcaga agagactgga acagatccac aaggccatgc accctgagct gcagtaccac     600

cctctggccc tgcccaaagt gcgggacgat ctgagcctgg acgcccggac cttcgacatc     660

ctgaacacca ccttcagact gctgcagatg agcaacttca gcctggccca ggactgctgg     720

ctgtgcctga agctgggcac ccctacacct ctggccatcc ctacccctag cctgacctac     780

tccctggccg atagcctggc caatgccagc tgccagatca tccccccact gctggtgcag     840

cccatgcagt tcagcaacag cagctgcctg agcagcccct tcatcaacga caccgagcag     900

atcgacctgg gcgccgtgac cttcaccaac tgtaccagcg tggccaacgt gtccagccct     960

ctgtgtgccc tgaacggcag cgtgttcctg tgcggcaaca acatggccta cacctacctg    1020

ccccagaact ggaccggcct gtgtgtgcag gcttccctgc tgcccgacat cgacatcatc    1080

cctggcgacg agcccgtgcc catccctgcc atcgatcact acatccaccg gcccaagcgg    1140

gccgtgcagt tcattcctct gctggccggc ctgggaatca ccgccgcttt tacaaccggc    1200

gccaccggac tgggcgtgtc cgtgacccag tacaccaagc tgagccacca gctgatctcc    1260

gacgtgcagg tgctgagcgg caccatccag gacctgcagg accaggtgga ctctctggcc    1320

gaagtggtgc tgcagaatcg gagaggcctg gacctgctga cagccgagca gggcggaatc    1380

tgtctggcac tgcaagaaaa gtgctgcttt tacgccaaca agagcggaat cgtgcggaac    1440

aagatccgca ccctgcagga agaactgcag aaaagaagag agagcctggc ctccaacccc    1500

ctgtggacag gcctgcaggg cttcctgcct tacctgctgc cactgctggg ccctctgctg    1560

accctgctgc tgatcctgac catcggcccc tgcgtgttca acaagctgat gaccttcatc    1620

aagcaccaga tcgagagcat ccaggccaag cccatccagg tgcactacca cagactggaa    1680

caggaagata gcggcggctc ttacctgacc ctgacctga                           1719


<210> 53
<211> 1653
<212> DNA
<213> Artificial Sequence


<220> 
<223> wild-type

<220> 
<221> misc_feature
<222> 1..6
<223> /note="additional nucleotides to form Kozak"

<400> 53
gccaccatga aactcccaac aggaatggtc attttatgta gcctaataat agttcgggca      60

gggtttgacg acccccgcaa ggctatcgca ttagtacaaa aacaacatgg taaaccatgc     120

gaatgcagcg gagggcaggt atccgaggcc ccaccgaact ccatccaaca ggtaacttgc     180

ccaggcaaga cggcctactt aatgaccaac caaaaatgga aatgcagagt cactccaaaa     240

aatctcaccc ctagcggggg agaactccag aactgcccct gtaacacttt ccaggactcg     300

atgcacagtt cttgttatac tgaataccgg caatgcaggg cgaataataa gacatactac     360

acggccacct tgcttaaaat acggtctggg agcctcaacg aggtacagat attacaaaac     420

cccaatcagc tcctacagtc cccttgtagg ggctctataa atcagcccgt ttgctggagt     480

gccacagccc ccatccatat ctccgatggt ggaggacccc tcgatactaa gagagtgtgg     540

acagtccaaa aaaggctaga acaaattcat aaggctatgc atcctgaact tcaataccac     600

cccttagccc tgcccaaagt cagagatgac cttagccttg atgcacggac ttttgatatc     660

ctgaatacca cttttaggtt actccagatg tccaatttta gccttgccca agattgttgg     720

ctctgtttaa aactaggtac ccctacccct cttgcgatac ccactccctc tttaacctac     780

tccctagcag actccctagc gaatgcctcc tgtcagatta tacctcccct cttggttcaa     840

ccgatgcagt tctccaactc gtcctgttta tcttcccctt tcattaacga tacggaacaa     900

atagacttag gtgcagtcac ctttactaac tgcacctctg tagccaatgt cagtagtcct     960

ttatgtgccc taaacgggtc agtcttcctc tgtggaaata acatggcata cacctattta    1020

ccccaaaact ggacaggact ttgcgtccaa gcctccctcc tccccgacat tgacatcatc    1080

ccgggggatg agccagtccc cattcctgcc attgatcatt atatacatag acctaaacga    1140

gctgtacagt tcatcccttt actagctgga ctgggaatca ccgcagcatt caccaccgga    1200

gctacaggcc taggtgtctc cgtcacccag tatacaaaat tatcccatca gttaatatct    1260

gatgtccaag tcttatccgg taccatacaa gatttacaag accaggtaga ctcgttagct    1320

gaagtagttc tccaaaatag gaggggactg gacctactaa cggcagaaca aggaggaatt    1380

tgtttagcct tacaagaaaa atgctgtttt tatgctaaca agtcaggaat tgtgagaaac    1440

aaaataagaa ccctacaaga agaattacaa aaacgcaggg aaagcctggc atccaaccct    1500

ctctggaccg ggctgcaggg ctttcttccg tacctcctac ctctcctggg acccctactc    1560

accctcctac tcatactaac cattgggcca tgcgttttca gtcgcctcat ggccttcatt    1620

aatgatagac ttaatgttgt acatgccatg tga                                 1653


<210> 54
<211> 1653
<212> DNA
<213> Artificial Sequence


<220> 
<223> codon-optimized

<220> 
<221> misc_feature
<222> 1..6
<223> /note="additional nucleotides to form Kozak"

<400> 54
gccaccatga aactcccaac aggaatggtc attttatgta gcctaataat agttcgggca      60

gggtttgacg acccccgcaa ggctatcgca ttagtacaaa aacaacatgg taaaccatgc     120

gaatgcagcg gagggcaggt atccgaggcc ccaccgaact ccatccaaca ggtaacttgc     180

ccaggcaaga cggcctactt aatgaccaac caaaaatgga aatgcagagt cactccaaaa     240

aatctcaccc ctagcggggg agaactccag aactgcccct gtaacacttt ccaggactcg     300

atgcacagtt cttgttatac tgaataccgg caatgcaggg cgaataataa gacatactac     360

acggccacct tgcttaaaat acggtctggg agcctcaacg aggtacagat attacaaaac     420

cccaatcagc tcctacagtc cccttgtagg ggctctataa atcagcccgt ttgctggagt     480

gccacagccc ccatccatat ctccgatggt ggaggacccc tcgatactaa gagagtgtgg     540

acagtccaaa aaaggctaga acaaattcat aaggctatgc atcctgaact tcaataccac     600

cccttagccc tgcccaaagt cagagatgac cttagccttg atgcacggac ttttgatatc     660

ctgaatacca cttttaggtt actccagatg tccaatttta gccttgccca agattgttgg     720

ctctgtttaa aactaggtac ccctacccct cttgcgatac ccactccctc tttaacctac     780

tccctagcag actccctagc gaatgcctcc tgtcagatta tacctcccct cttggttcaa     840

ccgatgcagt tctccaactc gtcctgttta tcttcccctt tcattaacga tacggaacaa     900

atagacttag gtgcagtcac ctttactaac tgcacctctg tagccaatgt cagtagtcct     960

ttatgtgccc taaacgggtc agtcttcctc tgtggaaata acatggcata cacctattta    1020

ccccaaaact ggacaggact ttgcgtccaa gcctccctcc tccccgacat tgacatcatc    1080

ccgggggatg agccagtccc cattcctgcc attgatcatt atatacatag acctaaacga    1140

gctgtacagt tcatcccttt actagctgga ctgggaatca ccgcagcatt caccaccgga    1200

gctacaggcc taggtgtctc cgtcacccag tatacaaaat tatcccatca gttaatatct    1260

gatgtccaag tcttatccgg taccatacaa gatttacaag accaggtaga ctcgttagct    1320

gaagtagttc tccaaaatag gaggggactg gacctactaa cggcagaaca aggaggaatt    1380

tgtttagcct tacaagaaaa atgctgtttt tatgctaaca agtcaggaat tgtgagaaac    1440

aaaataagaa ccctacaaga agaattacaa aaacgcaggg aaagcctggc atccaaccct    1500

ctctggaccg ggctgcaggg ctttcttccg tacctcctac ctctcctggg acccctactc    1560

accctcctac tcatactaac cattgggcca tgcgttttca acagactggt gcagttcgtg    1620

aaagaccgga tcagcgtggt gcaggctctg tga                                 1653


<210> 55
<211> 6910
<212> DNA
<213> Artificial Sequence


<220> 
<223> expression plasmid for glycoprotein

<220> 
<221> misc_feature
<222> 1..14
<223> /note="plasmid backbone"

<220> 
<221> promoter
<222> 15..766
<223> /note="CMV promoter"

<220> 
<221> intron
<222> 815..1362
<223> /note="beta-globin intron"

<220> 
<221> CDS
<222> 1425..3185
<223> /gene="encoding glycoprotein MPMVco"
      /transl_table=1

<220> 
<221> misc_feature
<222> 4068..6910
<223> /note="plasmid backbone"

<400> 55
gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca    60

tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt   120

aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat   180

aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa   240

taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg   300

agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc   360

cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct   420

tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga   480

tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa   540

gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc   600

caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg   660

aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac   720

gctgttttga cctccataga agacaccggg accgatccag cctccggtcg accgatcctg   780

agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat   840

tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct   900

tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac   960

cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc  1020

atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct  1080

aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga  1140

gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc  1200

gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt  1260

tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg  1320

gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc  1380

tggttgttgt gctgtctcat cattttggca aagaattcgc cacc atg aac ttc aac   1436
                                                 Met Asn Phe Asn 
                                                 1               

tac cac ttc atc tgg tcc ctc gtg atc ctg agc cag atc agc cag gtg    1484
Tyr His Phe Ile Trp Ser Leu Val Ile Leu Ser Gln Ile Ser Gln Val 
5                   10                  15                  20  

cag gcc ggc ttc ggc gat cct aga gaa gcc ctg gcc gag atc cag cag    1532
Gln Ala Gly Phe Gly Asp Pro Arg Glu Ala Leu Ala Glu Ile Gln Gln 
                25                  30                  35      

aag cac ggc aag ccc tgc gat tgc gct ggc ggc tat gtg tcc agc ccc    1580
Lys His Gly Lys Pro Cys Asp Cys Ala Gly Gly Tyr Val Ser Ser Pro 
            40                  45                  50          

ccc atc aat agc ctg acc acc gtg tcc tgc agc acc cac aca gcc tac    1628
Pro Ile Asn Ser Leu Thr Thr Val Ser Cys Ser Thr His Thr Ala Tyr 
        55                  60                  65              

agc gtg acc aac agc ctg aag tgg cag tgc gtg tcc acc ccc acc acc    1676
Ser Val Thr Asn Ser Leu Lys Trp Gln Cys Val Ser Thr Pro Thr Thr 
    70                  75                  80                  

cct agc aat acc cac atc ggc agc tgt ccc ggc gag tgc aac acc atc    1724
Pro Ser Asn Thr His Ile Gly Ser Cys Pro Gly Glu Cys Asn Thr Ile 
85                  90                  95                  100 

agc tac gac tct gtg cac gcc agc tgc tac aac cac tac cag cag tgc    1772
Ser Tyr Asp Ser Val His Ala Ser Cys Tyr Asn His Tyr Gln Gln Cys 
                105                 110                 115     

aac atc ggc aac aag acc tac ctg acc gcc acc atc acc ggc gac aga    1820
Asn Ile Gly Asn Lys Thr Tyr Leu Thr Ala Thr Ile Thr Gly Asp Arg 
            120                 125                 130         

aca ccc gcc atc ggc gac ggc aat gtg cct aca gtg ctg ggc acc agc    1868
Thr Pro Ala Ile Gly Asp Gly Asn Val Pro Thr Val Leu Gly Thr Ser 
        135                 140                 145             

cac aac ctg atc acc gcc ggc tgc ccc aac ggc aag aaa gga cag gtc    1916
His Asn Leu Ile Thr Ala Gly Cys Pro Asn Gly Lys Lys Gly Gln Val 
    150                 155                 160                 

gtg tgc tgg aac agc cgg ccc agc gtg cac atc tct gat ggc gga ggc    1964
Val Cys Trp Asn Ser Arg Pro Ser Val His Ile Ser Asp Gly Gly Gly 
165                 170                 175                 180 

cct cag gac aag gcc cgg gac atc atc gtg aac aag aag ttc gag gaa    2012
Pro Gln Asp Lys Ala Arg Asp Ile Ile Val Asn Lys Lys Phe Glu Glu 
                185                 190                 195     

ctg cac aga agc ctg ttc ccc gag ctg agc tac cac cct ctg gcc ctg    2060
Leu His Arg Ser Leu Phe Pro Glu Leu Ser Tyr His Pro Leu Ala Leu 
            200                 205                 210         

cct gag gcc aga ggc aaa gag aag atc gac gcc cac acc ctg gac ctg    2108
Pro Glu Ala Arg Gly Lys Glu Lys Ile Asp Ala His Thr Leu Asp Leu 
        215                 220                 225             

ctg gcc aca gtg cac agc ctg ctg aat gcc agc cag ccc tct ctg gcc    2156
Leu Ala Thr Val His Ser Leu Leu Asn Ala Ser Gln Pro Ser Leu Ala 
    230                 235                 240                 

gag gat tgc tgg ctg tgt ctg cag agc ggc gac cct gtg cca ctg gct    2204
Glu Asp Cys Trp Leu Cys Leu Gln Ser Gly Asp Pro Val Pro Leu Ala 
245                 250                 255                 260 

ctg ccc tac aac gac acc ctg tgc agc aac ttc gcc tgc ctg tcc aac    2252
Leu Pro Tyr Asn Asp Thr Leu Cys Ser Asn Phe Ala Cys Leu Ser Asn 
                265                 270                 275     

cac agc tgc ccc ctg acc cct cca ttc ctg gtg cag ccc ttc aac ttc    2300
His Ser Cys Pro Leu Thr Pro Pro Phe Leu Val Gln Pro Phe Asn Phe 
            280                 285                 290         

acc gac agc aac tgc ctg tac gcc cac tat cag aac aac agc ttc gac    2348
Thr Asp Ser Asn Cys Leu Tyr Ala His Tyr Gln Asn Asn Ser Phe Asp 
        295                 300                 305             

atc gac gtg ggc ctg gcc agc ttc acc aac tgc agc agc tac tac aat    2396
Ile Asp Val Gly Leu Ala Ser Phe Thr Asn Cys Ser Ser Tyr Tyr Asn 
    310                 315                 320                 

gtg tcc acc gcc agc aag ccc agc aat tcc ctg tgc gcc ccc aac agc    2444
Val Ser Thr Ala Ser Lys Pro Ser Asn Ser Leu Cys Ala Pro Asn Ser 
325                 330                 335                 340 

agc gtg ttc gtg tgc ggc aac aac aag gcc tac acc tac ctg ccc acc    2492
Ser Val Phe Val Cys Gly Asn Asn Lys Ala Tyr Thr Tyr Leu Pro Thr 
                345                 350                 355     

aac tgg acc ggc tct tgc gtg ctg gct acc ctg ctg ccc gac atc gat    2540
Asn Trp Thr Gly Ser Cys Val Leu Ala Thr Leu Leu Pro Asp Ile Asp 
            360                 365                 370         

atc atc ccc ggc agc gag ccc gtg ccc atc cct gcc att gat cac ttt    2588
Ile Ile Pro Gly Ser Glu Pro Val Pro Ile Pro Ala Ile Asp His Phe 
        375                 380                 385             

ctg ggc aag gcc aag cgg gcc atc cag ctg atc cct ctg ttt gtg gga    2636
Leu Gly Lys Ala Lys Arg Ala Ile Gln Leu Ile Pro Leu Phe Val Gly 
    390                 395                 400                 

ctg ggc atc acc acc gcc gtg tct aca ggc gct gct ggc ctg gga gtg    2684
Leu Gly Ile Thr Thr Ala Val Ser Thr Gly Ala Ala Gly Leu Gly Val 
405                 410                 415                 420 

tcc atc acc cag tac acc aag ctg tcc cac cag ctg att agc gac gtg    2732
Ser Ile Thr Gln Tyr Thr Lys Leu Ser His Gln Leu Ile Ser Asp Val 
                425                 430                 435     

cag gcc atc agc agc acc atc cag gac ctg cag gac cag gtg gac agc    2780
Gln Ala Ile Ser Ser Thr Ile Gln Asp Leu Gln Asp Gln Val Asp Ser 
            440                 445                 450         

ctg gca gaa gtg gtg ctg cag aac aga cgg ggc ctg gat ctg ctg aca    2828
Leu Ala Glu Val Val Leu Gln Asn Arg Arg Gly Leu Asp Leu Leu Thr 
        455                 460                 465             

gcc gag cag ggc gga att tgc ctg gca ctg cag gaa aag tgc tgc ttc    2876
Ala Glu Gln Gly Gly Ile Cys Leu Ala Leu Gln Glu Lys Cys Cys Phe 
    470                 475                 480                 

tac gcc aac aag agc ggc atc gtg cgg gac aag atc aag aat ctg cag    2924
Tyr Ala Asn Lys Ser Gly Ile Val Arg Asp Lys Ile Lys Asn Leu Gln 
485                 490                 495                 500 

gac gac ctg gaa cgg cgg agg cgg cag ctg atc gac aac cct ttc tgg    2972
Asp Asp Leu Glu Arg Arg Arg Arg Gln Leu Ile Asp Asn Pro Phe Trp 
                505                 510                 515     

acc agc ttc cac ggc ttt ctg cct tac gtg atg ccc ctg ctg ggc ccc    3020
Thr Ser Phe His Gly Phe Leu Pro Tyr Val Met Pro Leu Leu Gly Pro 
            520                 525                 530         

ctg ctg tgc ctg ctg ctg gtg ctg tct ttc ggc ccc atc atc ttc aac    3068
Leu Leu Cys Leu Leu Leu Val Leu Ser Phe Gly Pro Ile Ile Phe Asn 
        535                 540                 545             

aag ctg atg acc ttc atc aag cac cag atc gag agc atc cag gcc aag    3116
Lys Leu Met Thr Phe Ile Lys His Gln Ile Glu Ser Ile Gln Ala Lys 
    550                 555                 560                 

ccc atc cag gtg cac tac cac aga ctg gaa cag gaa gat agc ggc ggc    3164
Pro Ile Gln Val His Tyr His Arg Leu Glu Gln Glu Asp Ser Gly Gly 
565                 570                 575                 580 

tct tac ctg acc ctg acc tga gtcgacatgg tgctggccca gcaataccaa       3215
Ser Tyr Leu Thr Leu Thr                                      
                585                                          

gcactcaaag ctgaggaaga agctcaggat tgagcttccg ggacaaaagc aggggggaat  3275

gagaagtcag aaccccccac ctttgctaca taaataaccg ctttcatttc gcttctgtaa  3335

aaccgcttat gcgccccacc ctaaccgctt tcatttcgct tctgtaaaac cgcttatgcg  3395

ccccacccta gccggaaagt ccccagccgc tacgcaaccc gggccccgag ttgcatcagc  3455

cgcttcgcaa cccgggctcc gagttgcatc agccgaaaga aacttcattt cccaagcttc  3515

gagggatccg tcgaggaatt cactcctcag gtgcaggctg cctatcagaa ggtggtggct  3575

ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat  3635

tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt  3695

ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg  3755

gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat  3815

atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc  3875

cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata  3935

ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta  3995

gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg  4055

agatccctcg acggatcggc cgcaattcgt aatcatgtca tagctgtttc ctgtgtgaaa  4115

ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg  4175

gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca  4235

gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg  4295

tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg  4355

gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg  4415

ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa  4475

ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg  4535

acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc  4595

tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc  4655

ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc  4715

ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg  4775

ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc  4835

actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga  4895

gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc  4955

tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac  5015

caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg  5075

atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc  5135

acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa  5195

ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta  5255

ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt  5315

tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag  5375

tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca  5435

gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc  5495

tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt  5555

tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag  5615

ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggg  5675

ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca  5735

tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg  5795

tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct  5855

cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca  5915

tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca  5975

gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg  6035

tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac  6095

ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt  6155

attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc  6215

cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt  6275

cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat  6335

cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa  6395

gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg  6455

cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa  6515

agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc  6575

gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag  6635

tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg  6695

cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc  6755

ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac  6815

gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga  6875

ctcactatag ggcgaattgg agctccaccg cggtg                             6910

<210> 56
<211> 586
<212> PRT
<213> Artificial Sequence


<220> 
<223> [CDS]:1425..3185 from SEQ ID NO 55

<400> 56
Met Asn Phe Asn Tyr His Phe Ile Trp Ser Leu Val Ile Leu Ser Gln 
1               5                   10                  15      
Ile Ser Gln Val Gln Ala Gly Phe Gly Asp Pro Arg Glu Ala Leu Ala 
            20                  25                  30          
Glu Ile Gln Gln Lys His Gly Lys Pro Cys Asp Cys Ala Gly Gly Tyr 
        35                  40                  45              
Val Ser Ser Pro Pro Ile Asn Ser Leu Thr Thr Val Ser Cys Ser Thr 
    50                  55                  60                  
His Thr Ala Tyr Ser Val Thr Asn Ser Leu Lys Trp Gln Cys Val Ser 
65                  70                  75                  80  
Thr Pro Thr Thr Pro Ser Asn Thr His Ile Gly Ser Cys Pro Gly Glu 
                85                  90                  95      
Cys Asn Thr Ile Ser Tyr Asp Ser Val His Ala Ser Cys Tyr Asn His 
            100                 105                 110         
Tyr Gln Gln Cys Asn Ile Gly Asn Lys Thr Tyr Leu Thr Ala Thr Ile 
        115                 120                 125             
Thr Gly Asp Arg Thr Pro Ala Ile Gly Asp Gly Asn Val Pro Thr Val 
    130                 135                 140                 
Leu Gly Thr Ser His Asn Leu Ile Thr Ala Gly Cys Pro Asn Gly Lys 
145                 150                 155                 160 
Lys Gly Gln Val Val Cys Trp Asn Ser Arg Pro Ser Val His Ile Ser 
                165                 170                 175     
Asp Gly Gly Gly Pro Gln Asp Lys Ala Arg Asp Ile Ile Val Asn Lys 
            180                 185                 190         
Lys Phe Glu Glu Leu His Arg Ser Leu Phe Pro Glu Leu Ser Tyr His 
        195                 200                 205             
Pro Leu Ala Leu Pro Glu Ala Arg Gly Lys Glu Lys Ile Asp Ala His 
    210                 215                 220                 
Thr Leu Asp Leu Leu Ala Thr Val His Ser Leu Leu Asn Ala Ser Gln 
225                 230                 235                 240 
Pro Ser Leu Ala Glu Asp Cys Trp Leu Cys Leu Gln Ser Gly Asp Pro 
                245                 250                 255     
Val Pro Leu Ala Leu Pro Tyr Asn Asp Thr Leu Cys Ser Asn Phe Ala 
            260                 265                 270         
Cys Leu Ser Asn His Ser Cys Pro Leu Thr Pro Pro Phe Leu Val Gln 
        275                 280                 285             
Pro Phe Asn Phe Thr Asp Ser Asn Cys Leu Tyr Ala His Tyr Gln Asn 
    290                 295                 300                 
Asn Ser Phe Asp Ile Asp Val Gly Leu Ala Ser Phe Thr Asn Cys Ser 
305                 310                 315                 320 
Ser Tyr Tyr Asn Val Ser Thr Ala Ser Lys Pro Ser Asn Ser Leu Cys 
                325                 330                 335     
Ala Pro Asn Ser Ser Val Phe Val Cys Gly Asn Asn Lys Ala Tyr Thr 
            340                 345                 350         
Tyr Leu Pro Thr Asn Trp Thr Gly Ser Cys Val Leu Ala Thr Leu Leu 
        355                 360                 365             
Pro Asp Ile Asp Ile Ile Pro Gly Ser Glu Pro Val Pro Ile Pro Ala 
    370                 375                 380                 
Ile Asp His Phe Leu Gly Lys Ala Lys Arg Ala Ile Gln Leu Ile Pro 
385                 390                 395                 400 
Leu Phe Val Gly Leu Gly Ile Thr Thr Ala Val Ser Thr Gly Ala Ala 
                405                 410                 415     
Gly Leu Gly Val Ser Ile Thr Gln Tyr Thr Lys Leu Ser His Gln Leu 
            420                 425                 430         
Ile Ser Asp Val Gln Ala Ile Ser Ser Thr Ile Gln Asp Leu Gln Asp 
        435                 440                 445             
Gln Val Asp Ser Leu Ala Glu Val Val Leu Gln Asn Arg Arg Gly Leu 
    450                 455                 460                 
Asp Leu Leu Thr Ala Glu Gln Gly Gly Ile Cys Leu Ala Leu Gln Glu 
465                 470                 475                 480 
Lys Cys Cys Phe Tyr Ala Asn Lys Ser Gly Ile Val Arg Asp Lys Ile 
                485                 490                 495     
Lys Asn Leu Gln Asp Asp Leu Glu Arg Arg Arg Arg Gln Leu Ile Asp 
            500                 505                 510         
Asn Pro Phe Trp Thr Ser Phe His Gly Phe Leu Pro Tyr Val Met Pro 
        515                 520                 525             
Leu Leu Gly Pro Leu Leu Cys Leu Leu Leu Val Leu Ser Phe Gly Pro 
    530                 535                 540                 
Ile Ile Phe Asn Lys Leu Met Thr Phe Ile Lys His Gln Ile Glu Ser 
545                 550                 555                 560 
Ile Gln Ala Lys Pro Ile Gln Val His Tyr His Arg Leu Glu Gln Glu 
                565                 570                 575     
Asp Ser Gly Gly Ser Tyr Leu Thr Leu Thr 
            580                 585     

<210> 57
<211> 6477
<212> DNA
<213> Artificial Sequence


<220> 
<223> expression plasmid for glycoprotein

<220> 
<221> promoter
<222> 1..750
<223> /note="CMV promoter"

<220> 
<221> intron
<222> 801..1348
<223> /note="beta-globin intron"

<220> 
<221> CDS
<222> 1442..3202
<223> /note="encoding glycoprotein MPMVco"
      /transl_table=1

<220> 
<221> polyA_signal
<222> 3346..3882
<223> /note="beta-globin polyA"

<220> 
<221> misc_feature
<222> 3883..6477
<223> /note="plasmid backbone"

<400> 57
gagcttggcc cattgcatac gttgtatcca tatcataata tgtacattta tattggctca    60

tgtccaacat taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt   120

acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat   180

ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt   240

cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa   300

actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc   360

aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct   420

acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag   480

tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt   540

gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac   600

aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc   660

agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc   720

catagaagac accgggaccg atccagcctc cggtcgaccg atcctgagaa cttcagggtg   780

agtttgggga cccttgattg ttctttcttt ttcgctattg taaaattcat gttatatgga   840

gggggcaaag ttttcagggt gttgtttaga atgggaagat gtcccttgta tcaccatgga   900

ccctcatgat aattttgttt ctttcacttt ctactctgtt gacaaccatt gtctcctctt   960

attttctttt cattttctgt aactttttcg ttaaacttta gcttgcattt gtaacgaatt  1020

tttaaattca cttttgttta tttgtcagat tgtaagtact ttctctaatc actttttttt  1080

caaggcaatc agggtatatt atattgtact tcagcacagt tttagagaac aattgttata  1140

attaaatgat aaggtagaat atttctgcat ataaattctg gctggcgtgg aaatattctt  1200

attggtagaa acaactacac cctggtcatc atcctgcctt tctctttatg gttacaatga  1260

tatacactgt ttgagatgag gataaaatac tctgagtcca aaccgggccc ctctgctaac  1320

catgttcatg ccttcttctc tttcctacag ctcctgggca acgtgctggt tgttgtgctg  1380

tctcatcatt ttggcaaaga attcctcgac ggatccctcg aggaattctg acactgccac  1440

c atg aac ttc aac tac cac ttc atc tgg tcc ctc gtg atc ctg agc cag  1489
  Met Asn Phe Asn Tyr His Phe Ile Trp Ser Leu Val Ile Leu Ser Gln 
  1               5                   10                  15      

atc agc cag gtg cag gcc ggc ttc ggc gat cct aga gaa gcc ctg gcc    1537
Ile Ser Gln Val Gln Ala Gly Phe Gly Asp Pro Arg Glu Ala Leu Ala 
            20                  25                  30          

gag atc cag cag aag cac ggc aag ccc tgc gat tgc gct ggc ggc tat    1585
Glu Ile Gln Gln Lys His Gly Lys Pro Cys Asp Cys Ala Gly Gly Tyr 
        35                  40                  45              

gtg tcc agc ccc ccc atc aat agc ctg acc acc gtg tcc tgc agc acc    1633
Val Ser Ser Pro Pro Ile Asn Ser Leu Thr Thr Val Ser Cys Ser Thr 
    50                  55                  60                  

cac aca gcc tac agc gtg acc aac agc ctg aag tgg cag tgc gtg tcc    1681
His Thr Ala Tyr Ser Val Thr Asn Ser Leu Lys Trp Gln Cys Val Ser 
65                  70                  75                  80  

acc ccc acc acc cct agc aat acc cac atc ggc agc tgt ccc ggc gag    1729
Thr Pro Thr Thr Pro Ser Asn Thr His Ile Gly Ser Cys Pro Gly Glu 
                85                  90                  95      

tgc aac acc atc agc tac gac tct gtg cac gcc agc tgc tac aac cac    1777
Cys Asn Thr Ile Ser Tyr Asp Ser Val His Ala Ser Cys Tyr Asn His 
            100                 105                 110         

tac cag cag tgc aac atc ggc aac aag acc tac ctg acc gcc acc atc    1825
Tyr Gln Gln Cys Asn Ile Gly Asn Lys Thr Tyr Leu Thr Ala Thr Ile 
        115                 120                 125             

acc ggc gac aga aca ccc gcc atc ggc gac ggc aat gtg cct aca gtg    1873
Thr Gly Asp Arg Thr Pro Ala Ile Gly Asp Gly Asn Val Pro Thr Val 
    130                 135                 140                 

ctg ggc acc agc cac aac ctg atc acc gcc ggc tgc ccc aac ggc aag    1921
Leu Gly Thr Ser His Asn Leu Ile Thr Ala Gly Cys Pro Asn Gly Lys 
145                 150                 155                 160 

aaa gga cag gtc gtg tgc tgg aac agc cgg ccc agc gtg cac atc tct    1969
Lys Gly Gln Val Val Cys Trp Asn Ser Arg Pro Ser Val His Ile Ser 
                165                 170                 175     

gat ggc gga ggc cct cag gac aag gcc cgg gac atc atc gtg aac aag    2017
Asp Gly Gly Gly Pro Gln Asp Lys Ala Arg Asp Ile Ile Val Asn Lys 
            180                 185                 190         

aag ttc gag gaa ctg cac aga agc ctg ttc ccc gag ctg agc tac cac    2065
Lys Phe Glu Glu Leu His Arg Ser Leu Phe Pro Glu Leu Ser Tyr His 
        195                 200                 205             

cct ctg gcc ctg cct gag gcc aga ggc aaa gag aag atc gac gcc cac    2113
Pro Leu Ala Leu Pro Glu Ala Arg Gly Lys Glu Lys Ile Asp Ala His 
    210                 215                 220                 

acc ctg gac ctg ctg gcc aca gtg cac agc ctg ctg aat gcc agc cag    2161
Thr Leu Asp Leu Leu Ala Thr Val His Ser Leu Leu Asn Ala Ser Gln 
225                 230                 235                 240 

ccc tct ctg gcc gag gat tgc tgg ctg tgt ctg cag agc ggc gac cct    2209
Pro Ser Leu Ala Glu Asp Cys Trp Leu Cys Leu Gln Ser Gly Asp Pro 
                245                 250                 255     

gtg cca ctg gct ctg ccc tac aac gac acc ctg tgc agc aac ttc gcc    2257
Val Pro Leu Ala Leu Pro Tyr Asn Asp Thr Leu Cys Ser Asn Phe Ala 
            260                 265                 270         

tgc ctg tcc aac cac agc tgc ccc ctg acc cct cca ttc ctg gtg cag    2305
Cys Leu Ser Asn His Ser Cys Pro Leu Thr Pro Pro Phe Leu Val Gln 
        275                 280                 285             

ccc ttc aac ttc acc gac agc aac tgc ctg tac gcc cac tat cag aac    2353
Pro Phe Asn Phe Thr Asp Ser Asn Cys Leu Tyr Ala His Tyr Gln Asn 
    290                 295                 300                 

aac agc ttc gac atc gac gtg ggc ctg gcc agc ttc acc aac tgc agc    2401
Asn Ser Phe Asp Ile Asp Val Gly Leu Ala Ser Phe Thr Asn Cys Ser 
305                 310                 315                 320 

agc tac tac aat gtg tcc acc gcc agc aag ccc agc aat tcc ctg tgc    2449
Ser Tyr Tyr Asn Val Ser Thr Ala Ser Lys Pro Ser Asn Ser Leu Cys 
                325                 330                 335     

gcc ccc aac agc agc gtg ttc gtg tgc ggc aac aac aag gcc tac acc    2497
Ala Pro Asn Ser Ser Val Phe Val Cys Gly Asn Asn Lys Ala Tyr Thr 
            340                 345                 350         

tac ctg ccc acc aac tgg acc ggc tct tgc gtg ctg gct acc ctg ctg    2545
Tyr Leu Pro Thr Asn Trp Thr Gly Ser Cys Val Leu Ala Thr Leu Leu 
        355                 360                 365             

ccc gac atc gat atc atc ccc ggc agc gag ccc gtg ccc atc cct gcc    2593
Pro Asp Ile Asp Ile Ile Pro Gly Ser Glu Pro Val Pro Ile Pro Ala 
    370                 375                 380                 

att gat cac ttt ctg ggc aag gcc aag cgg gcc atc cag ctg atc cct    2641
Ile Asp His Phe Leu Gly Lys Ala Lys Arg Ala Ile Gln Leu Ile Pro 
385                 390                 395                 400 

ctg ttt gtg gga ctg ggc atc acc acc gcc gtg tct aca ggc gct gct    2689
Leu Phe Val Gly Leu Gly Ile Thr Thr Ala Val Ser Thr Gly Ala Ala 
                405                 410                 415     

ggc ctg gga gtg tcc atc acc cag tac acc aag ctg tcc cac cag ctg    2737
Gly Leu Gly Val Ser Ile Thr Gln Tyr Thr Lys Leu Ser His Gln Leu 
            420                 425                 430         

att agc gac gtg cag gcc atc agc agc acc atc cag gac ctg cag gac    2785
Ile Ser Asp Val Gln Ala Ile Ser Ser Thr Ile Gln Asp Leu Gln Asp 
        435                 440                 445             

cag gtg gac agc ctg gca gaa gtg gtg ctg cag aac aga cgg ggc ctg    2833
Gln Val Asp Ser Leu Ala Glu Val Val Leu Gln Asn Arg Arg Gly Leu 
    450                 455                 460                 

gat ctg ctg aca gcc gag cag ggc gga att tgc ctg gca ctg cag gaa    2881
Asp Leu Leu Thr Ala Glu Gln Gly Gly Ile Cys Leu Ala Leu Gln Glu 
465                 470                 475                 480 

aag tgc tgc ttc tac gcc aac aag agc ggc atc gtg cgg gac aag atc    2929
Lys Cys Cys Phe Tyr Ala Asn Lys Ser Gly Ile Val Arg Asp Lys Ile 
                485                 490                 495     

aag aat ctg cag gac gac ctg gaa cgg cgg agg cgg cag ctg atc gac    2977
Lys Asn Leu Gln Asp Asp Leu Glu Arg Arg Arg Arg Gln Leu Ile Asp 
            500                 505                 510         

aac cct ttc tgg acc agc ttc cac ggc ttt ctg cct tac gtg atg ccc    3025
Asn Pro Phe Trp Thr Ser Phe His Gly Phe Leu Pro Tyr Val Met Pro 
        515                 520                 525             

ctg ctg ggc ccc ctg ctg tgc ctg ctg ctg gtg ctg tct ttc ggc ccc    3073
Leu Leu Gly Pro Leu Leu Cys Leu Leu Leu Val Leu Ser Phe Gly Pro 
    530                 535                 540                 

atc atc ttc aac aag ctg atg acc ttc atc aag cac cag atc gag agc    3121
Ile Ile Phe Asn Lys Leu Met Thr Phe Ile Lys His Gln Ile Glu Ser 
545                 550                 555                 560 

atc cag gcc aag ccc atc cag gtg cac tac cac aga ctg gaa cag gaa    3169
Ile Gln Ala Lys Pro Ile Gln Val His Tyr His Arg Leu Glu Gln Glu 
                565                 570                 575     

gat agc ggc ggc tct tac ctg acc ctg acc tga ctcaaatcct gcacaacaga  3222
Asp Ser Gly Gly Ser Tyr Leu Thr Leu Thr                           
            580                 585                               

ttcttcatgt ttggaccaaa tcaacttgtg ataccatgct caaagaggcc tcaattatat  3282

ttgagttttt aatttttatg aaaaaaaaaa aaaaaaacgg aattcctcga gggatccgtc  3342

gaggaattca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat  3402

gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc  3462

atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata  3522

gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta  3582

aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc  3642

atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat  3702

tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg  3762

ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc  3822

ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac  3882

ggatcggccg caattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct  3942

cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg  4002

agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct  4062

gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg  4122

gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggtcaca  4182

aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt  4242

ttccccctgg aagctccctc gtgcgtcggt gtaggtcgtt cgctccaagc tgggctgtgt  4302

gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc  4362

caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag  4422

agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac  4482

tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt  4542

tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa  4602

gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg  4662

gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa  4722

aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat  4782

atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc  4842

gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat  4902

acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc  4962

ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc  5022

tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag  5082

ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg  5142

ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg  5202

atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag  5262

taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt  5322

catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga  5382

atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc  5442

acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc  5502

aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc  5562

ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc  5622

cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca  5682

atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat  5742

ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt  5802

gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt  5862

aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg  5922

ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc  5982

aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca  6042

agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga  6102

tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa  6162

ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc  6222

gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt  6282

tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt  6342

gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg  6402

acggccagtg agcgcgcgta atacgactca ctatagggcg aattggagct ccaccgcggt  6462

ggcggccgct ctaga                                                   6477

<210> 58
<211> 586
<212> PRT
<213> Artificial Sequence


<220> 
<223> [CDS]:1442..3202 from SEQ ID NO 57

<400> 58
Met Asn Phe Asn Tyr His Phe Ile Trp Ser Leu Val Ile Leu Ser Gln 
1               5                   10                  15      
Ile Ser Gln Val Gln Ala Gly Phe Gly Asp Pro Arg Glu Ala Leu Ala 
            20                  25                  30          
Glu Ile Gln Gln Lys His Gly Lys Pro Cys Asp Cys Ala Gly Gly Tyr 
        35                  40                  45              
Val Ser Ser Pro Pro Ile Asn Ser Leu Thr Thr Val Ser Cys Ser Thr 
    50                  55                  60                  
His Thr Ala Tyr Ser Val Thr Asn Ser Leu Lys Trp Gln Cys Val Ser 
65                  70                  75                  80  
Thr Pro Thr Thr Pro Ser Asn Thr His Ile Gly Ser Cys Pro Gly Glu 
                85                  90                  95      
Cys Asn Thr Ile Ser Tyr Asp Ser Val His Ala Ser Cys Tyr Asn His 
            100                 105                 110         
Tyr Gln Gln Cys Asn Ile Gly Asn Lys Thr Tyr Leu Thr Ala Thr Ile 
        115                 120                 125             
Thr Gly Asp Arg Thr Pro Ala Ile Gly Asp Gly Asn Val Pro Thr Val 
    130                 135                 140                 
Leu Gly Thr Ser His Asn Leu Ile Thr Ala Gly Cys Pro Asn Gly Lys 
145                 150                 155                 160 
Lys Gly Gln Val Val Cys Trp Asn Ser Arg Pro Ser Val His Ile Ser 
                165                 170                 175     
Asp Gly Gly Gly Pro Gln Asp Lys Ala Arg Asp Ile Ile Val Asn Lys 
            180                 185                 190         
Lys Phe Glu Glu Leu His Arg Ser Leu Phe Pro Glu Leu Ser Tyr His 
        195                 200                 205             
Pro Leu Ala Leu Pro Glu Ala Arg Gly Lys Glu Lys Ile Asp Ala His 
    210                 215                 220                 
Thr Leu Asp Leu Leu Ala Thr Val His Ser Leu Leu Asn Ala Ser Gln 
225                 230                 235                 240 
Pro Ser Leu Ala Glu Asp Cys Trp Leu Cys Leu Gln Ser Gly Asp Pro 
                245                 250                 255     
Val Pro Leu Ala Leu Pro Tyr Asn Asp Thr Leu Cys Ser Asn Phe Ala 
            260                 265                 270         
Cys Leu Ser Asn His Ser Cys Pro Leu Thr Pro Pro Phe Leu Val Gln 
        275                 280                 285             
Pro Phe Asn Phe Thr Asp Ser Asn Cys Leu Tyr Ala His Tyr Gln Asn 
    290                 295                 300                 
Asn Ser Phe Asp Ile Asp Val Gly Leu Ala Ser Phe Thr Asn Cys Ser 
305                 310                 315                 320 
Ser Tyr Tyr Asn Val Ser Thr Ala Ser Lys Pro Ser Asn Ser Leu Cys 
                325                 330                 335     
Ala Pro Asn Ser Ser Val Phe Val Cys Gly Asn Asn Lys Ala Tyr Thr 
            340                 345                 350         
Tyr Leu Pro Thr Asn Trp Thr Gly Ser Cys Val Leu Ala Thr Leu Leu 
        355                 360                 365             
Pro Asp Ile Asp Ile Ile Pro Gly Ser Glu Pro Val Pro Ile Pro Ala 
    370                 375                 380                 
Ile Asp His Phe Leu Gly Lys Ala Lys Arg Ala Ile Gln Leu Ile Pro 
385                 390                 395                 400 
Leu Phe Val Gly Leu Gly Ile Thr Thr Ala Val Ser Thr Gly Ala Ala 
                405                 410                 415     
Gly Leu Gly Val Ser Ile Thr Gln Tyr Thr Lys Leu Ser His Gln Leu 
            420                 425                 430         
Ile Ser Asp Val Gln Ala Ile Ser Ser Thr Ile Gln Asp Leu Gln Asp 
        435                 440                 445             
Gln Val Asp Ser Leu Ala Glu Val Val Leu Gln Asn Arg Arg Gly Leu 
    450                 455                 460                 
Asp Leu Leu Thr Ala Glu Gln Gly Gly Ile Cys Leu Ala Leu Gln Glu 
465                 470                 475                 480 
Lys Cys Cys Phe Tyr Ala Asn Lys Ser Gly Ile Val Arg Asp Lys Ile 
                485                 490                 495     
Lys Asn Leu Gln Asp Asp Leu Glu Arg Arg Arg Arg Gln Leu Ile Asp 
            500                 505                 510         
Asn Pro Phe Trp Thr Ser Phe His Gly Phe Leu Pro Tyr Val Met Pro 
        515                 520                 525             
Leu Leu Gly Pro Leu Leu Cys Leu Leu Leu Val Leu Ser Phe Gly Pro 
    530                 535                 540                 
Ile Ile Phe Asn Lys Leu Met Thr Phe Ile Lys His Gln Ile Glu Ser 
545                 550                 555                 560 
Ile Gln Ala Lys Pro Ile Gln Val His Tyr His Arg Leu Glu Gln Glu 
                565                 570                 575     
Asp Ser Gly Gly Ser Tyr Leu Thr Leu Thr 
            580                 585     

<210> 59
<211> 11035
<212> DNA
<213> Artificial Sequence


<220> 
<223> packaging plasmid for lentiviral Gag/Pol: pcDNA3.g/p.4xCTE

<220> 
<221> misc_feature
<222> 2..769
<223> /note="4xCTE"

<220> 
<221> polyA_signal
<222> 847..1071
<223> /note="bGH polyA"

<220> 
<221> misc_feature
<222> 1072..5481
<223> /note="plasmid backbone"

<220> 
<221> promoter
<222> 5482..6069
<223> /note="CMV promoter"

<220> 
<221> intron
<222> 6157..6700
<223> /note="beta globin intron"

<220> 
<221> gene
<222> 6722..11028
<223> /gene="HIV-1 gag/pol"

<400> 59
aattcctgca gcccgggatc cactagttct agaccacctc ccctgtgagc tagactggac       60

agccaatgac gggtaagaga gtgacatttc tcactaacct aagacaggag ggccgtcaaa      120

gctactgcct aatccaatga cgggtaatag tgacaagaaa tgtatcactc caacctaaga      180

caggcgcagc ctccgaggga tgtgttgatc agctagacca cctccctgtg agctagactg      240

gacagccaat gacgggtaag agagtgacat ttctcactaa cctaagacag gagggccgtc      300

aaagctactg cctaatccaa tgacgggtaa tagtgacaag aaatgtatca ctccaaccta      360

agacaggcgc agcctccgag ggatgtgttg atcagctaga ccacctcccc tgtgagctag      420

actggacagc caatgacggg taagagagtg acatttctca ctaacctaag acaggagggc      480

cgtcaaagct actgcctaat ccaatgacgg gtaatagtga caagaaatgt atcactccaa      540

cctaagacag gcgcagcctc cgagggatgt gttgatcagc tagaccacct cccctgtgag      600

ctagactgga cagccaatga cgggtaagag agtgacattt ctcactaacc taagacagga      660

gggccgtcaa agctactgcc taatccaatg acgggtaata gtgacaagaa atgtatcact      720

ccaacctaag acaggcgcag cctccgaggg atgtgttgat cagctagagc ggccgctcga      780

gcatgcatct agagggccct attctatagt gtcacctaaa tgctagagct cgctgatcag      840

cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct      900

tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc      960

attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg     1020

aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg     1080

cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa     1140

gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc     1200

ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag     1260

ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca     1320

aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc     1380

gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa     1440

cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct     1500

attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt     1560

gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat     1620

gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag     1680

tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat     1740

cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt     1800

tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg     1860

cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg     1920

atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc     1980

aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat     2040

cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt     2100

caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg     2160

gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag     2220

ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc     2280

tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc     2340

tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga     2400

agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga     2460

actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg     2520

cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg     2580

tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc     2640

tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc     2700

cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg     2760

gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc     2820

gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc     2880

ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct     2940

tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca     3000

ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg     3060

tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt     3120

tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt     3180

gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg     3240

ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg     3300

cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg     3360

cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat     3420

aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc     3480

gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc     3540

tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga     3600

agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt     3660

ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg     3720

taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc     3780

gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg     3840

gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc     3900

ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg     3960

ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc     4020

gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct     4080

caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt     4140

taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa     4200

aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa     4260

tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc     4320

tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct     4380

gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca     4440

gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt     4500

aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt     4560

gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc     4620

ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc     4680

tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt     4740

atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact     4800

ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc     4860

ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt     4920

ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg     4980

atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct     5040

gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa     5100

tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt     5160

ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc     5220

acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat     5280

ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg     5340

cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag     5400

gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg     5460

atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa     5520

ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa     5580

atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg     5640

ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt     5700

aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg     5760

tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc     5820

ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc     5880

agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca     5940

ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta     6000

acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa     6060

gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga     6120

ctcactatag ggagacccaa gcttggtacc gagctcggat cctgagaact tcagggtgag     6180

tctatgggac ccttgatgtt ttctttcccc ttcttttcta tggttaagtt catgtcatag     6240

gaaggggaga agtaacaggg tacacatatt gaccaaatca gggtaatttt gcatttgtaa     6300

ttttaaaaaa tgctttcttc ttttaatata cttttttgtt tatcttattt ctaatacttt     6360

ccctaatctc tttctttcag ggcaataatg atacaatgta tcatgcctct ttgcaccatt     6420

ctaaagaata acagtgataa tttctgggtt aaggcaatag caatatttct gcatataaat     6480

atttctgcat ataaattgta actgatgtaa gaggtttcat attgctaata gcagctacaa     6540

tccagctacc attctgcttt tattttatgg ttgggataag gctggattat tctgagtcca     6600

agctaggccc ttttgctaat catgttcata cctcttatct tcctcccaca gctcctgggc     6660

aacgtgctgg tctgtgtgct ggcccatcac tttggcaaag aattcgagat ctgccgccgc     6720

catgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgatggg aaaaaattcg     6780

gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga     6840

gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat     6900

actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa     6960

tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc     7020

tttagacaag atagaggaag agcaaaacaa aagtaagaaa aaagcacagc aagcagcagc     7080

tgacacagga cacagcaatc aggtcagcca aaattaccct atagtgcaga acatccaggg     7140

gcaaatggta catcaggcca tatcacctag aactttaaat gcatgggtaa aagtagtaga     7200

agagaaggct ttcagcccag aagtgatacc catgttttca gcattatcag aaggagccac     7260

cccacaagat ttaaacacca tgctaaacac agtgggggga catcaagcag ccatgcaaat     7320

gttaaaagag accatcaatg aggaagctgc agaatgggat agagtgcatc cagtgcatgc     7380

agggcctatt gcaccaggcc agatgagaga accaagggga agtgacatag caggaactac     7440

tagtaccctt caggaacaaa taggatggat gacacataat ccacctatcc cagtaggaga     7500

aatctataaa agatggataa tcctgggatt aaataaaata gtaagaatgt atagccctac     7560

cagcattctg gacataagac aaggaccaaa ggaacccttt agagactatg tagaccgatt     7620

ctataaaact ctaagagccg agcaagcttc acaagaggta aaaaattgga tgacagaaac     7680

cttgttggtc caaaatgcga acccagattg taagactatt ttaaaagcat tgggaccagg     7740

agcgacacta gaagaaatga tgacagcatg tcagggagtg gggggacccg gccataaagc     7800

aagagttttg gctgaagcaa tgagccaagt aacaaatcca gctaccataa tgatacagaa     7860

aggcaatttt aggaaccaaa gaaagactgt taagtgtttc aattgtggca aagaagggca     7920

catagccaaa aattgcaggg cccctaggaa aaagggctgt tggaaatgtg gaaaggaagg     7980

acaccaaatg aaagattgta ctgagagaca ggctaatttt ttagggaaga tctggccttc     8040

ccacaaggga aggccaggga attttcttca gagcagacca gagccaacag ccccaccaga     8100

agagagcttc aggtttgggg aagagacaac aactccctct cagaagcagg agccgataga     8160

caaggaactg tatcctttag cttccctcag atcactcttt ggcagcgacc cctcgtcaca     8220

ataaagatag gggggcaatt aaaggaagct ctattagata caggagcaga tgatacagta     8280

ttagaagaaa tgaatttgcc aggaagatgg aaaccaaaaa tgataggggg aattggaggt     8340

tttatcaaag taagacagta tgatcagata ctcatagaaa tctgcggaca taaagctata     8400

ggtacagtat tagtaggacc tacacctgtc aacataattg gaagaaatct gttgactcag     8460

attggctgca ctttaaattt tcccattagt cctattgaga ctgtaccagt aaaattaaag     8520

ccaggaatgg atggcccaaa agttaaacaa tggccattga cagaagaaaa aataaaagca     8580

ttagtagaaa tttgtacaga aatggaaaag gaaggaaaaa tttcaaaaat tgggcctgaa     8640

aatccataca atactccagt atttgccata aagaaaaaag acagtactaa atggagaaaa     8700

ttagtagatt tcagagaact taataagaga actcaagatt tctgggaagt tcaattagga     8760

ataccacatc ctgcagggtt aaaacagaaa aaatcagtaa cagtactgga tgtgggcgat     8820

gcatattttt cagttccctt agataaagac ttcaggaagt atactgcatt taccatacct     8880

agtataaaca atgagacacc agggattaga tatcagtaca atgtgcttcc acagggatgg     8940

aaaggatcac cagcaatatt ccagtgtagc atgacaaaaa tcttagagcc ttttagaaaa     9000

caaaatccag acatagtcat ctatcaatac atggatgatt tgtatgtagg atctgactta     9060

gaaatagggc agcatagaac aaaaatagag gaactgagac aacatctgtt gaggtgggga     9120

tttaccacac cagacaaaaa acatcagaaa gaacctccat tcctttggat gggttatgaa     9180

ctccatcctg ataaatggac agtacagcct atagtgctgc cagaaaagga cagctggact     9240

gtcaatgaca tacagaaatt agtgggaaaa ttgaattggg caagtcagat ttatgcaggg     9300

attaaagtaa ggcaattatg taaacttctt aggggaacca aagcactaac agaagtagta     9360

ccactaacag aagaagcaga gctagaactg gcagaaaaca gggagattct aaaagaaccg     9420

gtacatggag tgtattatga cccatcaaaa gacttaatag cagaaataca gaagcagggg     9480

caaggccaat ggacatatca aatttatcaa gagccattta aaaatctgaa aacaggaaag     9540

tatgcaagaa tgaagggtgc ccacactaat gatgtgaaac aattaacaga ggcagtacaa     9600

aaaatagcca cagaaagcat agtaatatgg ggaaagactc ctaaatttaa attacccata     9660

caaaaggaaa catgggaagc atggtggaca gagtattggc aagccacctg gattcctgag     9720

tgggagtttg tcaatacccc tcccttagtg aagttatggt accagttaga gaaagaaccc     9780

ataataggag cagaaacttt ctatgtagat ggggcagcca atagggaaac taaattagga     9840

aaagcaggat atgtaactga cagaggaaga caaaaagttg tccccctaac ggacacaaca     9900

aatcagaaga ctgagttaca agcaattcat ctagctttgc aggattcggg attagaagta     9960

aacatagtga cagactcaca atatgcattg ggaatcattc aagcacaacc agataagagt    10020

gaatcagagt tagtcagtca aataatagag cagttaataa aaaaggaaaa agtctacctg    10080

gcatgggtac cagcacacaa aggaattgga ggaaatgaac aagtagataa attggtcagt    10140

gctggaatca ggaaagtact atttttagat ggaatagata aggcccaaga agaacatgag    10200

aaatatcaca gtaattggag agcaatggct agtgatttta acctaccacc tgtagtagca    10260

aaagaaatag tagccagctg tgataaatgt cagctaaaag gggaagccat gcatggacaa    10320

gtagactgta gcccaggaat atggcagcta gattgtacac atttagaagg aaaagttatc    10380

ttggtagcag ttcatgtagc cagtggatat atagaagcag aagtaattcc agcagagaca    10440

gggcaagaaa cagcatactt cctcttaaaa ttagcaggaa gatggccagt aaaaacagta    10500

catacagaca atggcagcaa tttcaccagt actacagtta aggccgcctg ttggtgggcg    10560

gggatcaagc aggaatttgg cattccctac aatccccaaa gtcaaggagt aatagaatct    10620

atgaataaag aattaaagaa aattatagga caggtaagag atcaggctga acatcttaag    10680

acagcagtac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg    10740

tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta    10800

caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca    10860

gtttggaaag gaccagcaaa gctcctctgg aaaggtgaag gggcagtagt aatacaagat    10920

aatagtgaca taaaagtagt gccaagaaga aaagcaaaga tcatcaggga ttatggaaaa    10980

cagatggcag gtgatgattg tgtggcaagt agacaggatg aggattaaca catgg         11035


<210> 60
<211> 4180
<212> DNA
<213> Artificial Sequence


<220> 
<223> Packaging plasmid for Rev: pRSV_Rev

<220> 
<221> misc_feature
<222> 1..237
<223> /note="plasmid backbone"

<220> 
<221> promoter
<222> 238..635
<223> /note="RSV promoter"

<220> 
<221> CDS
<222> 756..1106
<223> /note="Rev"
      /transl_table=1

<220> 
<221> misc_feature
<222> 1107..4180
<223> /note="plasmid backbone"

<400> 60
agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc    60

acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc   120

tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa   180

ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg aattcgatgt   240

acgggccaga tatacgcgta tctgagggga ctagggtgtg tttaggcgaa aagcggggct   300

tcggttgtac gcggttagga gtcccctcag gatatagtag tttcgctttt gcatagggag   360

ggggaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag   420

caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg   480

tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact   540

gaattccgca ttgcagagat attgtattta agtgcctagc tcgatacaat aaacgccatt   600

tgaccattca ccacattggt gtgcacctcc aagctcgagc tcgtttagtg aaccgtcaga   660

tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca   720

gcctcccctc gaagctagtc gattaggcat ctcct atg gca gga aga agc gga      773
                                       Met Ala Gly Arg Ser Gly 
                                       1               5       

gac agc gac gaa gac ctc ctc aag gca gtc aga ctc atc aag ttt ctc     821
Asp Ser Asp Glu Asp Leu Leu Lys Ala Val Arg Leu Ile Lys Phe Leu 
            10                  15                  20          

tat caa agc aac cca cct ccc aat ccc gag ggg acc cga cag gcc cga     869
Tyr Gln Ser Asn Pro Pro Pro Asn Pro Glu Gly Thr Arg Gln Ala Arg 
        25                  30                  35              

agg aat aga aga aga agg tgg aga gag aga cag aga cag atc cat tcg     917
Arg Asn Arg Arg Arg Arg Trp Arg Glu Arg Gln Arg Gln Ile His Ser 
    40                  45                  50                  

att agt gaa cgg atc ctt agc act tat ctg gga cga tct gcg gag cct     965
Ile Ser Glu Arg Ile Leu Ser Thr Tyr Leu Gly Arg Ser Ala Glu Pro 
55                  60                  65                  70  

gtg cct ctt cag cta cca ccg ctt gag aga ctt act ctt gat tgt aac    1013
Val Pro Leu Gln Leu Pro Pro Leu Glu Arg Leu Thr Leu Asp Cys Asn 
                75                  80                  85      

gag gat tgt gga act tct ggg acg cag ggg gtg gga agc cct caa ata    1061
Glu Asp Cys Gly Thr Ser Gly Thr Gln Gly Val Gly Ser Pro Gln Ile 
            90                  95                  100         

ttg gtg gaa tct cct aca ata ttg gag tca gga gct aaa gaa tag        1106
Leu Val Glu Ser Pro Thr Ile Leu Glu Ser Gly Ala Lys Glu     
        105                 110                 115         

tgctgttagc ttgctcaatg ccacagctat agcagtagct gaggggacag atagggttat  1166

agaagtagta caagaagctt ggcactggcc gtcgttttac aacgtcgtga tctgagcctg  1226

ggagatctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt  1286

gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat caggaaaacc  1346

ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata  1406

gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc  1466

gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa  1526

gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg  1586

cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc  1646

ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg  1706

gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc  1766

acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt  1826

ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc  1886

ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta  1946

acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct  2006

cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc  2066

tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt  2126

ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa  2186

gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac  2246

gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat  2306

acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg  2366

aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc  2426

attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga  2486

tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga  2546

gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg  2606

cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc  2666

tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac  2726

agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact  2786

tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca  2846

tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg  2906

tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact  2966

acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg  3026

accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg  3086

tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat  3146

cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc  3206

tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat  3266

actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt  3326

tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc  3386

cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt  3446

gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac  3506

tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt  3566

gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct  3626

gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga  3686

ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac  3746

acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg  3806

agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt  3866

cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc  3926

tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg  3986

gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc  4046

ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc  4106

ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag  4166

cgaggaagcg gaag                                                    4180

<210> 61
<211> 116
<212> PRT
<213> Artificial Sequence


<220> 
<223> [CDS]:756..1106 from SEQ ID NO 60

<400> 61
Met Ala Gly Arg Ser Gly Asp Ser Asp Glu Asp Leu Leu Lys Ala Val 
1               5                   10                  15      
Arg Leu Ile Lys Phe Leu Tyr Gln Ser Asn Pro Pro Pro Asn Pro Glu 
            20                  25                  30          
Gly Thr Arg Gln Ala Arg Arg Asn Arg Arg Arg Arg Trp Arg Glu Arg 
        35                  40                  45              
Gln Arg Gln Ile His Ser Ile Ser Glu Arg Ile Leu Ser Thr Tyr Leu 
    50                  55                  60                  
Gly Arg Ser Ala Glu Pro Val Pro Leu Gln Leu Pro Pro Leu Glu Arg 
65                  70                  75                  80  
Leu Thr Leu Asp Cys Asn Glu Asp Cys Gly Thr Ser Gly Thr Gln Gly 
                85                  90                  95      
Val Gly Ser Pro Gln Ile Leu Val Glu Ser Pro Thr Ile Leu Glu Ser 
            100                 105                 110         
Gly Ala Lys Glu 
        115     

<210> 62
<211> 7712
<212> DNA
<213> Artificial Sequence


<220> 
<223> Lentiviral vector transfer plasmid: pRRL.PPT.CAGs.dTomato.pre

<220> 
<221> misc_feature
<222> 1..3
<223> /note="plasmid backbone"

<220> 
<221> promoter
<222> 4..235
<223> /note="RSV promoter"

<220> 
<221> misc_feature
<222> 236..417
<223> /note="RU5"

<220> 
<221> primer_bind
<222> 418..435
<223> /note="PBS"

<220> 
<221> gene
<222> 572..936
<223> /note="dGag"

<220> 
<221> misc_RNA
<222> 1082..1323
<223> /note="RRE"

<220> 
<221> protein_bind
<222> 1807..1930
<223> /note="PPT"

<220> 
<221> enhancer
<222> 1965..2341
<223> /note="CMVie"

<220> 
<221> promoter
<222> 1965..2820
<223> /note="CAGs promoter (CMV enhancer, chicken beta-Actin promoter
      and rabbit beta-Globin splice acceptor site)"

<220> 
<221> promoter
<222> 2343..2620
<223> /note="chicken beta-actin promoter"

<220> 
<221> CDS
<222> 2846..3550
<223> /note="dTomato (reporter transgene)"
      /transl_table=1

<220> 
<221> enhancer
<222> 3551..4159
<223> /note="PRE"

<220> 
<221> LTR
<222> 4241..4474
<223> /note="SIN LTR"

<220> 
<221> misc_feature
<222> 4475..7712
<223> /note="plasmid backbone"

<220> 
<221> polyA_signal
<222> 6368..6588
<223> /note="bGH polyA"

<400> 62
agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag    60

caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg   120

tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact   180

gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacaa taaacgggtc   240

tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct   300

taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga   360

ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg   420

cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact   480

cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa   540

attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg   600

gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat   660

aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc   720

ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag   780

acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat   840

caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac   900

aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat   960

gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg  1020

agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat  1080

aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcctcaat  1140

gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt  1200

gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca  1260

gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat  1320

ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag  1380

taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat  1440

taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa  1500

gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat  1560

aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt  1620

aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt  1680

atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga  1740

agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta  1800

tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt  1860

agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca  1920

aaattttatc gatcacgaga ctagcctcga gctagcccgt cgacattgat tattgactag  1980

ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt  2040

tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac  2100

gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg  2160

ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag  2220

tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat  2280

gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat  2340

ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc  2400

aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg  2460

ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggagagg  2520

tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg  2580

gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgcgctgcct  2640

tcgccccgtg ccccgctccg cgccgcctcg cgccgcccgc cccggctctg actgaccgcg  2700

ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg  2760

gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag  2820

gaccggtgaa ttcggatccg ccacc atg gtg agc aag ggc gag gag gtc atc    2872
                            Met Val Ser Lys Gly Glu Glu Val Ile 
                            1               5                   

aaa gag ttc atg cgc ttc aag gtg cgc atg gag ggc tcc atg aac ggc    2920
Lys Glu Phe Met Arg Phe Lys Val Arg Met Glu Gly Ser Met Asn Gly 
10                  15                  20                  25  

cac gag ttc gag atc gag ggc gag ggc gag ggc cgc ccc tac gag ggc    2968
His Glu Phe Glu Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly 
                30                  35                  40      

acc cag acc gcc aag ctg aag gtg acc aag ggc ggc ccc ctg ccc ttc    3016
Thr Gln Thr Ala Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe 
            45                  50                  55          

gcc tgg gac atc ctg tcc ccc cag ttc atg tac ggc tcc aag gcg tac    3064
Ala Trp Asp Ile Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr 
        60                  65                  70              

gtg aag cac ccc gcc gac atc ccc gat tac aag aag ctg tcc ttc ccc    3112
Val Lys His Pro Ala Asp Ile Pro Asp Tyr Lys Lys Leu Ser Phe Pro 
    75                  80                  85                  

gag ggc ttc aag tgg gag cgc gtg atg aac ttc gag gac ggc ggt ctg    3160
Glu Gly Phe Lys Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Leu 
90                  95                  100                 105 

gtg acc gtg acc cag gac tcc tcc ctg cag gac ggc acg ctg atc tac    3208
Val Thr Val Thr Gln Asp Ser Ser Leu Gln Asp Gly Thr Leu Ile Tyr 
                110                 115                 120     

aag gtg aag atg cgc ggc acc aac ttc ccc ccc gac ggc ccc gta atg    3256
Lys Val Lys Met Arg Gly Thr Asn Phe Pro Pro Asp Gly Pro Val Met 
            125                 130                 135         

cag aag aag acc atg ggc tgg gag gcc tcc acc gag cgc ctg tac ccc    3304
Gln Lys Lys Thr Met Gly Trp Glu Ala Ser Thr Glu Arg Leu Tyr Pro 
        140                 145                 150             

cgc gac ggc gtg ctg aag ggc gag atc cac cag gcc ctg aag ctg aag    3352
Arg Asp Gly Val Leu Lys Gly Glu Ile His Gln Ala Leu Lys Leu Lys 
    155                 160                 165                 

gac ggc ggc cac tac ctg gtg gag ttc aag acc atc tac atg gcc aag    3400
Asp Gly Gly His Tyr Leu Val Glu Phe Lys Thr Ile Tyr Met Ala Lys 
170                 175                 180                 185 

aag ccc gtg caa ctg ccc ggc tac tac tac gtg gac acc aag ctg gac    3448
Lys Pro Val Gln Leu Pro Gly Tyr Tyr Tyr Val Asp Thr Lys Leu Asp 
                190                 195                 200     

atc acc tcc cac aac gag gac tac acc atc gtg gaa cag tac gag cgc    3496
Ile Thr Ser His Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg 
            205                 210                 215         

tcc gag ggc cgc cac cac ctg ttc ctg tac ggc atg gac gag ctg tac    3544
Ser Glu Gly Arg His His Leu Phe Leu Tyr Gly Met Asp Glu Leu Tyr 
        220                 225                 230             

aag taa agcggccgcg tcgacaatca acctctggat tacaaaattt gtgaaagatt     3600
Lys                                                            
                                                               

gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc  3660

tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg  3720

gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac  3780

tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc  3840

cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc  3900

ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa  3960

gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc  4020

cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc  4080

ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg  4140

ggccgcctcc ccgcctggaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca  4200

gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc  4260

caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga  4320

gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct  4380

tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc  4440

agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt  4500

cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca  4560

gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt  4620

tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc  4680

tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa  4740

ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt  4800

gaggaggctt ttttggaggc ctaggctttt gcgtcgagac gtacccaatt cgccctatag  4860

tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc  4920

tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag  4980

cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg  5040

cgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac  5100

cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc  5160

cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt  5220

tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg  5280

gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag  5340

tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt  5400

ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt  5460

taacgcgaat tttaacaaaa tattaacgtt tacaatttcc caggtggcac ttttcgggga  5520

aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc  5580

atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt  5640

caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct  5700

cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt  5760

tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt  5820

tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac  5880

gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac  5940

tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct  6000

gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg  6060

aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg  6120

gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca  6180

atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa  6240

caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt  6300

ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc  6360

attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg  6420

agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt  6480

aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt  6540

catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc  6600

ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct  6660

tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta  6720

ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc  6780

ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac  6840

ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct  6900

gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat  6960

aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg  7020

acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa  7080

gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg  7140

gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga  7200

cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc  7260

aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct  7320

gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct  7380

cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca  7440

atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg  7500

tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat  7560

taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc  7620

ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac  7680

cctcactaaa gggaacaaaa gctggagctg ca                                7712

<210> 63
<211> 10768
<212> DNA
<213> Artificial Sequence


<220> 
<223> Packaging plasmid for alpharetroviral Gag/Pol: pcDNA3.ASLV gp co

<220> 
<221> promoter
<222> 232..819
<223> /note="CMV promoter"

<220> 
<221> intron
<222> 907..1463
<223> /note="beta globin intron"

<220> 
<221> gene
<222> 1475..6291
<223> /note="gag/pol from ASLV (codon-optimized)"

<220> 
<221> misc_feature
<222> 6589..10768
<223> /note="plasmid backbone"

<400> 63
gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc      180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt      240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata      300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      420

attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt      480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca      840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc      900

gagctcggat cctgagaact tcagggtgag tctatgggac ccttgatgtt ttctttcccc      960

ttcttttcta tggttaagtt catgtcatag gaaggggaga agtaacaggg tacacatatt     1020

gaccaaatca gggtaatttt gcatttgtaa ttttaaaaaa tgctttcttc ttttaatata     1080

cttttttgtt tatcttattt ctaatacttt ccctaatctc tttctttcag ggcaataatg     1140

atacaatgta tcatgcctct ttgcaccatt ctaaagaata acagtgataa tttctgggtt     1200

aaggcaatag caatatttct gcatataaat atttctgcat ataaattgta actgatgtaa     1260

gaggtttcat attgctaata gcagctacaa tccagctacc attctgcttt tattttatgg     1320

ttgggataag gctggattat tctgagtcca agctaggccc ttttgctaat catgttcata     1380

cctcttatct tcctcccaca gctcctgggc aacgtgctgg tctgtgtgct ggcccatcac     1440

tttggcaaag cacgtgagat ctgaattcgc caccatggaa gccgtgatca aagtgatcag     1500

cagcgcctgc aagacctact gcggcaagat cagccccagc aagaaagaaa tcggcgctat     1560

gctgtctctc ctgcaaaagg aaggcctgct gatgagcccc agcgacctgt acagccccgg     1620

cagctgggat cctatcacag ccgccctgag ccagagggcc atggtgctgg gcaagagcgg     1680

cgagctgaaa acctgggggc tggtgctggg agccctgaag gccgccagag aagaacaggt     1740

gaccagcgag caggccaagt tttggctggg cctgggcgga ggaagagtgt ctccccctgg     1800

ccccgagtgt atcgagaagc ccgccaccga gcggagaatc gacaagggcg aggaagtggg     1860

cgagacaacc gtgcagcggg acgccaagat ggcccctgag gaagccgcca cccctaagac     1920

cgtgggcacc agctgctacc actgtggcac cgccgtgggc tgtaattgtg ccaccgccac     1980

cgcctctgcc cctcctcctc cttacgtggg cagcggcctg tatccttctc tggccggcgt     2040

gggcgagcag cagggacagg gcgacaatac cagcagaggc gccgagcagc ctagagagga     2100

acctggacac gctggccagg ccccaggacc tgccctgaca gattgggcca gagtgcggga     2160

ggaactggcc tctaccggac cccctgtggt ggccatgccc gtggtgatca agacagaggg     2220

ccctgcctgg acccctctgg aacccaagct gatcacccgg ctggccgata cagtgcggac     2280

caagggcctg agaagcccca tcaccatggc cgaggtggag gccctgatga gcagccccct     2340

gctgccccac gacgtgacca acctgatgag agtgatcctg ggccctgctc cctacgccct     2400

gtggatggat gcctggggcg tgcagctgca gacagtgatc gccgctgcca ccagagatcc     2460

cagacacccc gccaatggcc agggcagagg cgagcggacc aacctggaca gactgaaggg     2520

cctggccgac ggcatggtgg gcaatcctca gggacaggcc gctctgctga ggcctggcga     2580

actggtggcc atcacagcca gcgccctgca ggccttcaga gaggtggcca gactggccga     2640

gcctgccggc ccttgggccg atatcaccca gggccccagc gagagcttcg tggacttcgc     2700

caaccggctg atcaaggccg tggagggcag cgatctgcct cctagcgcca gagcccccgt     2760

gatcatcgac tgcttccggc agaagtccca gcccgacatc cagcagctga tcagagccgc     2820

ccctagcacc ctgaccaccc ctggcgagat catcaaatac gtgctggacc ggcagaagat     2880

cgcccctctg accgatcagg gcattgccgc cgctatgagc agcgccatcc agcctctggt     2940

gatggccgtg gtgaacagag agagggacgg ccagacagga tctggcggca gagccagagg     3000

cctgtgctac acctgtggca gccctggcca ctaccaggcc cagtgcccca agaagcggaa     3060

gtccggcaac agccgggaga gatgccagct gtgtgacggc atgggccaca acgccaagca     3120

gtgccggaag cgggatggca atcagggcca gaggcccggc agaggactgt ctagcggccc     3180

ttggcctgga cctgagcagc ctgccgtgag cctggccatg accatggaac acaaggaccg     3240

gcccctggtg cgcgtgatcc tgaccaacac cggcagccac cccgtgaagc agcggagcgt     3300

gtacatcacc gccctgctgg acagcggagc cgacatcacc atcatcagcg aagaggactg     3360

gcccaccgac tggcctgtgg tggacaccgc caacccccag atccacggca tcggcggagg     3420

aatccccatg cggaagtccc gggatatgat agaggtgggg gttattaacc gagacgggtc     3480

gttggagcga cccctgctcc tcttccccgc agtcgctatg gttagaggga gtatcctagg     3540

aagagattgt ctgcagggcc tagggctccg cttgacaaat ttatagggag ggccactgtt     3600

cttactgttg cgctacatct ggctattccg ctcaaatgga agtcagaccg cacgcctgtg     3660

tggattgacc agtggcccct tcctgaaggt aaacttgtag cgctaacgca attagtggaa     3720

aaagaattac agttaggcca catcgagccc agcctgagct gctggaacac ccccgtgttc     3780

gtgatcagaa aggccagcgg cagctacaga ctgctgcacg acctgcgggc cgtgaatgcc     3840

aagctggtgc cctttggcgc cgtgcagcag ggcgctcctg tgctgtctgc cctgccaaga     3900

ggatggcccc tgatggtgct ggacctgaag gactgcttct tcagcatccc tctggccgag     3960

caggacagag aggccttcgc cttcaccctg cctagcgtga acaaccaggc cccagccaga     4020

cggttccagt ggaaggtgct gccccagggc atgacctgca gccccaccat ctgtcagctg     4080

gtcgtgggac aggtgctgga acccctgaga ctgaagcacc ccgccctgcg gatgctgcac     4140

tacatggacg acctgctcct cgccgcttct agccacgacg gcctggaagc cgccggaaaa     4200

gaagtgatcg gcaccctgga aagagccggc ttcaccatca gccccgacaa gatccagagg     4260

gaacccggcg tgcagtacct gggctacaag ctgggcagca cctatgtggc ccctgtgggc     4320

ctggtggccg agccaagaat cgccaccctg tgggacgtgc agaaactggt gggcagcctg     4380

cagtggctga ggccagccct gggcatccct cccagactga tgggcccctt ctacgagcag     4440

ctgcggggct ccgaccctaa tgaggcccgg gagtggaacc tggatatgaa gatggcctgg     4500

cgggagatcg tgcagctgtc caccactgcc gccctggaac gctgggatcc agcacagcca     4560

ctcgaaggtg ccgtggccag atgtgaacag ggcgccatcg gcgtgctggg ccagggactg     4620

agcacccacc ccagaccctg cctgtggctg ttcagcaccc agcccaccaa ggccttcacc     4680

gcctggctgg aagtgctgac cctgctgatc accaagctgc gggccagcgc cgtgagaacc     4740

ttcggcaaag aggtggacat cctgctgctg ccagcctgct ttagagagga cctgcccctg     4800

cctgagggaa tcctgctggc cctgagaggc tttgccggca agatccggtc cagcgacacc     4860

cccagcatct tcgatatcgc cagacccctg cacgtgtccc tgaaagtgcg cgtgaccgat     4920

caccctgtgc ctggccccac cgtgtttacc gacgccagca gcagcaccca caagggcgtg     4980

gtggtgtgga gagaaggccc cagatgggag atcaaagaaa tcgtggacct gggcgcctct     5040

gtgcagcagc tggaagccag agccgtggcc atggctctgc tgctgtggcc caccaccccc     5100

accaacgtgg tgaccgacag cgccttcgtg gccaagatgc tgctgaagat gggccaggaa     5160

ggcgtcccta gcaccgccgc tgccttcatc ctggaagatg ccctgtccca gcggagcgct     5220

atggctgctg tgctgcacgt gcggagccac agcgaggtgc ccggcttctt caccgagggc     5280

aacgacgtgg ccgacagcca ggccaccttc caggcctacc ccctgagaga ggccaaggac     5340

ctgcacacag ccctgcacat cggccccaga gccctgagca aggcctgcaa catcagcatg     5400

cagcaggcca gagaagtggt gcagacctgt ccccactgta atagcgcccc tgctctggaa     5460

gccggcgtga accctagagg cctcggccct ctgcagatct ggcagaccga cttcaccctg     5520

gaaccccgga tggcccctag aagctggctg gccgtgaccg tggataccgc cagctccgcc     5580

atcgtggtga cccagcacgg cagagtgaca tctgtggccg cccagcacca ctgggccaca     5640

gccattgccg tgctgggcag acccaaggcc atcaagaccg acaacggcag ctgcttcacc     5700

agccggtcca ccagagaatg gctggccaga tggggcattg ctcacaccac cggcatccca     5760

ggcaatagtc agggccaggc catggtggag agagccaaca ggctgctgaa ggacaagatc     5820

cgggtgctgg ccgagggcga cggcttcatg aagcggatcc ccgccagcaa acagggcgag     5880

ctgctggcca aggccatgta cgccctgaac cacttcgagc ggggcgagaa caccaagacc     5940

cccgtgcaga agcactggcg gcctaccgtg ctgacagagg gaccccccgt gaagatccgg     6000

atcgagacag gcgagtggga gaaggggtgg aacgtgctgg tgtggggcag aggctacgcc     6060

gctgtcaaga accgggacac cgacaaagtg atctgggtgc ccagccggaa agtgaagccc     6120

gacgtgaccc agaaagacga agtgaccaag aaggacgagg cctcccccct ctttgccggc     6180

atcagcgact ggatcccttg ggaggacgag caggaaggcc tgcagggcga gacagccagc     6240

aacaagcagg aaagacccgg cgaggacacc ctggccgcca atgagagctg agaattctgc     6300

agatatccag cacagtggcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca     6360

gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc     6420

ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg     6480

cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg     6540

gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag     6600

gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta     6660

agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg     6720

cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa     6780

gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc     6840

aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt     6900

cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca     6960

acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc     7020

tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg     7080

tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca     7140

tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa     7200

gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca     7260

tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt     7320

ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag     7380

gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg     7440

gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg     7500

caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa     7560

tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg     7620

tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt     7680

ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa     7740

gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc     7800

ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg     7860

ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg     7920

aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg     7980

aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg     8040

gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact     8100

gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg     8160

ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc     8220

ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct     8280

ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac     8340

cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat     8400

cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc     8460

ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc     8520

actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc     8580

gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg     8640

ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg     8700

tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc     8760

gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt     8820

gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct     8880

gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga     8940

taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc     9000

cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg     9060

ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg     9120

aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt     9180

tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt     9240

gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg     9300

cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact     9360

ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt     9420

cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct     9480

gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac     9540

cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca     9600

agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta     9660

agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa     9720

atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg     9780

cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg     9840

actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc     9900

aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc     9960

cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa    10020

ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc    10080

cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg    10140

ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc    10200

cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat    10260

ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg    10320

tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc    10380

ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg    10440

aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat    10500

gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg    10560

gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg    10620

ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct    10680

catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac    10740

atttccccga aaagtgccac ctgacgtc                                       10768


<210> 64
<211> 5881
<212> DNA
<213> Artificial Sequence


<220> 
<223> Alpharetroviral vector transfer plasmid: pCAS SF GFPpre SIN
      noTATA

<220> 
<221> promoter
<222> 446..1116
<223> /note="CMV promoter"

<220> 
<221> repeat_unit
<222> 1117..1137
<223> /note="R"

<220> 
<221> 5'UTR
<222> 1138..1217
<223> /note="U5"

<220> 
<221> primer_bind
<222> 1218..1236
<223> /note="PBS"

<220> 
<221> promoter
<222> 1488..1879
<223> /note="SFFV promoter"

<220> 
<221> gene
<222> 1957..2676
<223> /note="EGFP (reporter transgene)"

<220> 
<221> enhancer
<222> 2715..3317
<223> /note="PRE*"

<220> 
<221> 3'UTR
<222> 3475..3528
<223> /note="dU3 (with SIN deletion)"

<220> 
<221> LTR
<222> 3475..3629
<223> /note="SIN LTR"

<220> 
<221> repeat_region
<222> 3529..3549
<223> /note="R"

<220> 
<221> 5'UTR
<222> 3550..3629
<223> /note="U5"

<400> 64
ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac       60

ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc      120

gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gttactatgc      180

ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg      240

cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga      300

agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc      360

aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc      420

cagtgaatta gtactctagc ttaagccatt gcatacgttg tatccatatc ataatatgta      480

catttatatt ggctcatgtc caacattacc gccatgttga cattgattat tgactagtta      540

ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac      600

ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc      660

aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt      720

ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac      780

gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac      840

cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt      900

gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc      960

aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt     1020

tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg     1080

ggaggtctat ataagcagag ctcgtttagt gaaccggcca tttgaccatt caccacattg     1140

gtgtgcacct gggttgatgg ccggaccgtt gattccctga cgactacgag cacctgcatg     1200

aagcagaagg cttcatttgg tgaccccgac gtgatagtta gggaatagtg gtcggccaca     1260

gacggcgtgg cgatcctgtc tccatccgtc tcgtctatcg ggaggcgagt tcgatgaccc     1320

tggtggaggg ggctgcggct tagggaggca gaagctgagt accgtcggag ggagctccag     1380

ggcccggagc gactgacccc tgccgagaac tcagagggtc gtcggaagac ggagagtgag     1440

cccgacgacc accccaggca cgtctttggt cggcctgcgg atcaagcagc ggccgctagc     1500

tgcagtaacg ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag     1560

atcaagggcg ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc     1620

agtttcggcc ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg     1680

aggccaagaa cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat     1740

cagatgtttc caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc     1800

aatcagcctg cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc     1860

tcacaacccc tcactcggcg cgccagtcct ccgacagact gagtcggccg gtcgaatcaa     1920

gcttatcggg ccgctctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg     1980

ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc     2040

agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc     2100

tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc     2160

gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc     2220

atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag     2280

acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc     2340

atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc     2400

cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc     2460

cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc     2520

atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg     2580

agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc     2640

gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcgtcga cggatccccc     2700

gggctgcagg aattcgagca tcttaccgcc atttataccc atatttgttc tgtttttctt     2760

gatttgggta tacatttaaa tgttaataaa acaaaatggt ggggcaatca tttacatttt     2820

tagggatatg taattactag ttcaggtgta ttgccacaag acaaacatgt taagaaactt     2880

tcccgttatt tacgctctgt tcctgttaat caacctctgg attacaaaat ttgtgaaaga     2940

ttgactgata ttcttaacta tgttgctcct tttacgctgt gtggatatgc tgctttaatg     3000

cctctgtatc atgctattgc ttcccgtacg gctttcgttt tctcctcctt gtataaatcc     3060

tggttgctgt ctctttatga ggagttgtgg cccgttgtcc gtcaacgtgg cgtggtgtgc     3120

tctgtgtttg ctgacgcaac ccccactggc tggggcattg ccaccacctg tcaactcctt     3180

tctgggactt tcgctttccc cctcccgatc gccacggcag aactcatcgc cgcctgcctt     3240

gcccgctgct ggacaggggc taggttgctg ggcactgata attccgtggt gttgtcgggg     3300

aagctgacgt cctttcgaat tcgaaagctt ttaaatatcg atgcgatgta cgggccagat     3360

atacgcgtat ctgaggggac tagggtgtgt ttaggcgaaa agcggggctt cggttgtacg     3420

cggttaggag tccccttagg atatagtagt ttcgcttttg catagggagg gggaaatgta     3480

gtcttatgca atactcttgt acgtagtgcc tagctcgata caataaacgc catttgacca     3540

ttcaccacat tggtgtgcac ctgggttgat ggccggaccg ttgattccct gacgactacg     3600

agcacctgca tgaagcagaa ggcttcattc tcgagagctt tggcgtaatc atggtcatag     3660

ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc     3720

ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc     3780

tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa     3840

cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg     3900

ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg     3960

ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag     4020

gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac     4080

gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga     4140

taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt     4200

accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc     4260

tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc     4320

cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta     4380

agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat     4440

gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca     4500

gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct     4560

tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt     4620

acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct     4680

cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc     4740

acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa     4800

acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta     4860

tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc     4920

ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat     4980

ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta     5040

tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt     5100

aatagtttgc gcaacgttgt tgccattgct gctggcatcg tggtgtcacg ctcgtcgttt     5160

ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg     5220

ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc     5280

gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc     5340

gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg     5400

cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga     5460

actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta     5520

ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct     5580

tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag     5640

ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga     5700

agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat     5760

aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc     5820

attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa     5880

g                                                                     5881


<210> 65
<211> 234
<212> PRT
<213> Artificial Sequence


<220> 
<223> [CDS]:2846..3550 from SEQ ID NO 62

<400> 65
Met Val Ser Lys Gly Glu Glu Val Ile Lys Glu Phe Met Arg Phe Lys 
1               5                   10                  15      
Val Arg Met Glu Gly Ser Met Asn Gly His Glu Phe Glu Ile Glu Gly 
            20                  25                  30          
Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala Lys Leu Lys 
        35                  40                  45              
Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro 
    50                  55                  60                  
Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His Pro Ala Asp Ile 
65                  70                  75                  80  
Pro Asp Tyr Lys Lys Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg 
                85                  90                  95      
Val Met Asn Phe Glu Asp Gly Gly Leu Val Thr Val Thr Gln Asp Ser 
            100                 105                 110         
Ser Leu Gln Asp Gly Thr Leu Ile Tyr Lys Val Lys Met Arg Gly Thr 
        115                 120                 125             
Asn Phe Pro Pro Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp 
    130                 135                 140                 
Glu Ala Ser Thr Glu Arg Leu Tyr Pro Arg Asp Gly Val Leu Lys Gly 
145                 150                 155                 160 
Glu Ile His Gln Ala Leu Lys Leu Lys Asp Gly Gly His Tyr Leu Val 
                165                 170                 175     
Glu Phe Lys Thr Ile Tyr Met Ala Lys Lys Pro Val Gln Leu Pro Gly 
            180                 185                 190         
Tyr Tyr Tyr Val Asp Thr Lys Leu Asp Ile Thr Ser His Asn Glu Asp 
        195                 200                 205             
Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ser Glu Gly Arg His His Leu 
    210                 215                 220                 
Phe Leu Tyr Gly Met Asp Glu Leu Tyr Lys 
225                 230                 

