                         SEQUENCE LISTING

<110>  Institut National de la recherche scientifique
 
<120>  GALECTIN-1-SPECIFIC MONOVALENT ANTIBODIES AND USES THEREOF

<130>  G16398-00045

<150>  63/201,569
<151>  2021-05-05

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR1 of hsd2Ab

<400>  1

Ser Thr Phe Ser Glu Asp Ala 
1               5           


<210>  2
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR2 of hsd2Ab

<400>  2

Gly Phe Ala Asn Pro His Ser 
1               5           


<210>  3
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ASHKKTRAPAATFET

<400>  3

Ala Ser His Lys Lys Thr Arg Ala Pro Ala Ala Thr Phe Glu Thr 
1               5                   10                  15  


<210>  4
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FR1 of hsd2Ab

<400>  4

Met Ala Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Phe Val Gln Pro 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
            20                  25              


<210>  5
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FR2 of hsd2Ab

<400>  5

Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val Ser 
1               5                   10                  15      


Ala Ile Ser 
            


<210>  6
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FR3 of hsd2Ab

<400>  6

Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
1               5                   10                  15      


Ser Lys Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
            20                  25                  30          


Thr Ala Thr Tyr Tyr Cys Ala 
        35                  


<210>  7
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FR4 of hsd2Ab

<400>  7

Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
1               5                   10          


<210>  8
<211>  127
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  full sequence of G1N1 VHH

<400>  8

Met Ala Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Phe Val Gln Pro 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Thr Phe Ser 
            20                  25                  30          


Glu Asp Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu 
        35                  40                  45              


Phe Val Ser Ala Ile Ser Gly Phe Ala Asn Pro His Ser Tyr Tyr Ala 
    50                  55                  60                  


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn 
65                  70                  75                  80  


Thr Val Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Thr 
                85                  90                  95      


Tyr Tyr Cys Ala Ala Ser His Lys Lys Thr Arg Ala Pro Ala Ala Thr 
            100                 105                 110         


Phe Glu Thr Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125         


<210>  9
<211>  124
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  G1N2 VHH

<400>  9

Met Ala Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Phe Val Gln Pro 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Thr Tyr Phe 
            20                  25                  30          


His Thr Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu 
        35                  40                  45              


Phe Val Ser Ala Ile Ser Gly Phe Ala Asn Pro His Arg Tyr Tyr Ala 
    50                  55                  60                  


Ser Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn 
65                  70                  75                  80  


Thr Val Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Thr 
                85                  90                  95      


Tyr Tyr Cys Ala Asn Leu Ile Ser Thr Asn Arg Gly Trp Leu Met Gly 
            100                 105                 110         


Tyr Trp Cys Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 


<210>  10
<211>  135
<212>  PRT
<213>  Homo sapiens

<400>  10

Met Ala Cys Gly Leu Val Ala Ser Asn Leu Asn Leu Lys Pro Gly Glu 
1               5                   10                  15      


Cys Leu Arg Val Arg Gly Glu Val Ala Pro Asp Ala Lys Ser Phe Val 
            20                  25                  30          


Leu Asn Leu Gly Lys Asp Ser Asn Asn Leu Cys Leu His Phe Asn Pro 
        35                  40                  45              


Arg Phe Asn Ala His Gly Asp Ala Asn Thr Ile Val Cys Asn Ser Lys 
    50                  55                  60                  


Asp Gly Gly Ala Trp Gly Thr Glu Gln Arg Glu Ala Val Phe Pro Phe 
65                  70                  75                  80  


Gln Pro Gly Ser Val Ala Glu Val Cys Ile Thr Phe Asp Gln Ala Asn 
                85                  90                  95      


Leu Thr Val Lys Leu Pro Asp Gly Tyr Glu Phe Lys Phe Pro Asn Arg 
            100                 105                 110         


Leu Asn Leu Glu Ala Ile Asn Tyr Met Ala Ala Asp Gly Asp Phe Lys 
        115                 120                 125             


Ile Lys Cys Val Ala Phe Asp 
    130                 135 


<210>  11
<211>  139
<212>  PRT
<213>  Homo sapiens

<400>  11

Met Ser Ser Leu Pro Val Pro Tyr Lys Leu Pro Val Ser Leu Ser Val 
1               5                   10                  15      


Gly Ser Cys Val Ile Ile Lys Gly Thr Pro Ile His Ser Phe Ile Asn 
            20                  25                  30          


Asp Pro Gln Leu Gln Val Asp Phe Tyr Thr Asp Met Asp Glu Asp Ser 
        35                  40                  45              


Asp Ile Ala Phe Arg Phe Arg Val His Phe Gly Asn His Val Val Met 
    50                  55                  60                  


Asn Arg Arg Glu Phe Gly Ile Trp Met Leu Glu Glu Thr Thr Asp Tyr 
65                  70                  75                  80  


Val Pro Phe Glu Asp Gly Lys Gln Phe Glu Leu Cys Ile Tyr Val His 
                85                  90                  95      


Tyr Asn Glu Tyr Glu Ile Lys Val Asn Gly Ile Arg Ile Tyr Gly Phe 
            100                 105                 110         


Val His Arg Ile Pro Pro Ser Phe Val Lys Met Val Gln Val Ser Arg 
        115                 120                 125             


Asp Ile Ser Leu Thr Ser Val Cys Val Cys Asn 
    130                 135                 


<210>  12
<211>  142
<212>  PRT
<213>  Homo sapiens

<400>  12

Met Ser Phe Leu Thr Val Pro Tyr Lys Leu Pro Val Ser Leu Ser Val 
1               5                   10                  15      


Gly Ser Cys Val Ile Ile Lys Gly Thr Leu Ile Asp Ser Ser Ile Asn 
            20                  25                  30          


Glu Pro Gln Leu Gln Val Asp Phe Tyr Thr Glu Met Asn Glu Asp Ser 
        35                  40                  45              


Glu Ile Ala Phe His Leu Arg Val His Leu Gly Arg Arg Val Val Met 
    50                  55                  60                  


Asn Ser Arg Glu Phe Gly Ile Trp Met Leu Glu Glu Asn Leu His Tyr 
65                  70                  75                  80  


Val Pro Phe Glu Asp Gly Lys Pro Phe Asp Leu Arg Ile Tyr Val Cys 
                85                  90                  95      


Leu Asn Glu Tyr Glu Val Lys Val Asn Gly Glu Tyr Ile Tyr Ala Phe 
            100                 105                 110         


Val His Arg Ile Pro Pro Ser Tyr Val Lys Met Ile Gln Val Trp Arg 
        115                 120                 125             


Asp Val Ser Leu Asp Ser Val Leu Val Asn Asn Gly Arg Arg 
    130                 135                 140         


<210>  13
<211>  136
<212>  PRT
<213>  Homo sapiens

<400>  13

Met Ser Asn Val Pro His Lys Ser Ser Leu Pro Glu Gly Ile Arg Pro 
1               5                   10                  15      


Gly Thr Val Leu Arg Ile Arg Gly Leu Val Pro Pro Asn Ala Ser Arg 
            20                  25                  30          


Phe His Val Asn Leu Leu Cys Gly Glu Glu Gln Gly Ser Asp Ala Ala 
        35                  40                  45              


Leu His Phe Asn Pro Arg Leu Asp Thr Ser Glu Val Val Phe Asn Ser 
    50                  55                  60                  


Lys Glu Gln Gly Ser Trp Gly Arg Glu Glu Arg Gly Pro Gly Val Pro 
65                  70                  75                  80  


Phe Gln Arg Gly Gln Pro Phe Glu Val Leu Ile Ile Ala Ser Asp Asp 
                85                  90                  95      


Gly Phe Lys Ala Val Val Gly Asp Ala Gln Tyr His His Phe Arg His 
            100                 105                 110         


Arg Leu Pro Leu Ala Arg Val Arg Leu Val Glu Val Gly Gly Asp Val 
        115                 120                 125             


Gln Leu Asp Ser Val Arg Ile Phe 
    130                 135     


<210>  14
<211>  132
<212>  PRT
<213>  Homo sapiens

<400>  14

Met Thr Gly Glu Leu Glu Val Lys Asn Met Asp Met Lys Pro Gly Ser 
1               5                   10                  15      


Thr Leu Lys Ile Thr Gly Ser Ile Ala Asp Gly Thr Asp Gly Phe Val 
            20                  25                  30          


Ile Asn Leu Gly Gln Gly Thr Asp Lys Leu Asn Leu His Phe Asn Pro 
        35                  40                  45              


Arg Phe Ser Glu Ser Thr Ile Val Cys Asn Ser Leu Asp Gly Ser Asn 
    50                  55                  60                  


Trp Gly Gln Glu Gln Arg Glu Asp His Leu Cys Phe Ser Pro Gly Ser 
65                  70                  75                  80  


Glu Val Lys Phe Thr Val Thr Phe Glu Ser Asp Lys Phe Lys Val Lys 
                85                  90                  95      


Leu Pro Asp Gly His Glu Leu Thr Phe Pro Asn Arg Leu Gly His Ser 
            100                 105                 110         


His Leu Ser Tyr Leu Ser Val Arg Gly Gly Phe Asn Met Ser Ser Phe 
        115                 120                 125             


Lys Leu Lys Glu 
    130         


<210>  15
<211>  135
<212>  PRT
<213>  Mus musculus

<400>  15

Met Ala Cys Gly Leu Val Ala Ser Asn Leu Asn Leu Lys Pro Gly Glu 
1               5                   10                  15      


Cys Leu Lys Val Arg Gly Glu Val Ala Ser Asp Ala Lys Ser Phe Val 
            20                  25                  30          


Leu Asn Leu Gly Lys Asp Ser Asn Asn Leu Cys Leu His Phe Asn Pro 
        35                  40                  45              


Arg Phe Asn Ala His Gly Asp Ala Asn Thr Ile Val Cys Asn Thr Lys 
    50                  55                  60                  


Glu Asp Gly Thr Trp Gly Thr Glu His Arg Glu Pro Ala Phe Pro Phe 
65                  70                  75                  80  


Gln Pro Gly Ser Ile Thr Glu Val Cys Ile Thr Phe Asp Gln Ala Asp 
                85                  90                  95      


Leu Thr Ile Lys Leu Pro Asp Gly His Glu Phe Lys Phe Pro Asn Arg 
            100                 105                 110         


Leu Asn Met Glu Ala Ile Asn Tyr Met Ala Ala Asp Gly Asp Phe Lys 
        115                 120                 125             


Ile Lys Cys Val Ala Phe Glu 
    130                 135 


