                         SEQUENCE LISTING

<110>  GREENLIGHT BIOSCIENCES, INC.
       ABSHIRE, JAMES ROBBINS
       DHAMANKAR, HIMANSHU
       DAVITT, CHRISTOPHER J.H.
       HUDSON, MICHAEL
       GREGG, CHRISTOPHER
       AULISA, LORENZO
       JAIN, RACHIT
       SAMSA, MARCELO
       FARMER, WILLIAM
       DE, NABANITA
       HILL, IAN
 
<120>  MESSENGER RNA THERAPEUTICS AND COMPOSITIONS

<130>  GLB-001PC/128041-5001

<140>  PCT/US2022/027290
<141>  2022-05-02

<150>  US 63/182,290
<151>  2021-04-30

<150>  US 63/253,481
<151>  2021-10-07

<160>  35    

<170>  PatentIn version 3.5

<210>  1
<211>  3822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Full length ORF (DNA template) encoding SARS-CoV2 spike protein 
       (Esp3I linearized template)

<400>  1
atgttcgtgt tcctggtgct gctgccgctg gtgagcagcc agtgcgtgaa cctgacgacg       60

cggacgcagc tgccgccggc gtacacgaac agcttcacgc ggggggtgta ctacccggac      120

aaggtgttcc ggagcagcgt gctgcacagc acgcaggacc tgttcctgcc gttcttcagc      180

aacgtgacgt ggttccacgc gatccacgtg agcgggacga acgggacgaa gcggttcgac      240

aacccggtgc tgccgttcaa cgacggggtg tacttcgcga gcacggagaa gagcaacatc      300

atccgggggt ggatcttcgg gacgacgctg gacagcaaga cgcagagcct gctgatcgtg      360

aacaacgcga cgaacgtggt gatcaaggtg tgcgagttcc agttctgcaa cgacccgttc      420

ctgggggtgt actaccacaa gaacaacaag agctggatgg agagcgagtt ccgggtgtac      480

agcagcgcga acaactgcac gttcgagtac gtgagccagc cgttcctgat ggacctggag      540

gggaagcagg ggaacttcaa gaacctgcgg gagttcgtgt tcaagaacat cgacgggtac      600

ttcaagatct acagcaagca cacgccgatc aacctggtgc gggacctgcc gcaggggttc      660

agcgcgctgg agccgctggt ggacctgccg atcgggatca acatcacgcg gttccagacg      720

ctgctggcgc tgcaccggag ctacctgacg ccgggggaca gcagcagcgg gtggacggcg      780

ggggcggcgg cgtactacgt ggggtacctg cagccgcgga cgttcctgct gaagtacaac      840

gagaacggga cgatcacgga cgcggtggac tgcgcgctgg acccgctgag cgagaccaag      900

tgcacgctga agagcttcac ggtggagaag gggatctacc agacgagcaa cttccgggtg      960

cagccgacgg agagcatcgt gcggttcccg aacatcacga acctgtgccc gttcggggag     1020

gtgttcaacg cgacgcggtt cgcgagcgtg tacgcgtgga accggaagcg gatcagcaac     1080

tgcgtggcgg actacagcgt gctgtacaac agcgcgagct tcagcacgtt caagtgctac     1140

ggggtgagcc cgacgaagct gaacgacctg tgcttcacga acgtgtacgc ggacagcttc     1200

gtgatccggg gggacgaggt gcggcagatc gcgccggggc agacggggaa gatcgcggac     1260

tacaactaca agctgccgga cgacttcacg gggtgcgtga tcgcgtggaa cagcaacaac     1320

ctggacagca aggtgggggg gaactacaac tacctgtacc ggctgttccg gaagagcaac     1380

ctgaagccgt tcgagcggga catcagcacg gagatctacc aggcggggag cacgccgtgc     1440

aacggggtgg aggggttcaa ctgctacttc ccgctgcaga gctacgggtt ccagccgacg     1500

aacggggtgg ggtaccagcc gtaccgggtg gtggtgctga gcttcgagct gctgcacgcg     1560

ccggcgacgg tgtgcgggcc gaagaagagc acgaacctgg tgaagaacaa gtgcgtgaac     1620

ttcaacttca acgggctgac ggggacgggg gtgctgacgg agagcaacaa gaagttcctg     1680

ccgttccagc agttcgggcg ggacatcgcg gacacgacgg acgcggtgcg ggacccgcag     1740

acgctggaga tcctggacat cacgccgtgc agcttcgggg gggtgagcgt gatcacgccg     1800

gggacgaaca cgagcaacca ggtggcggtg ctgtaccagg acgtgaactg cacggaggtg     1860

ccggtggcga tccacgcgga ccagctgacg ccgacgtggc gggtgtacag cacggggagc     1920

aacgtgttcc agacgcgggc ggggtgcctg atcggggcgg agcacgtgaa caacagctac     1980

gagtgcgaca tcccgatcgg ggcggggatc tgcgcgagct accagacgca gacgaacagc     2040

ccgcggcggg cgcggagcgt ggcgagccag agcatcatcg cgtacacgat gagcctgggg     2100

gcggagaaca gcgtggcgta cagcaacaac agcatcgcga tcccgacgaa cttcacgatc     2160

agcgtgacga cggagatcct gccggtgagc atgacgaaga cgagcgtgga ctgcacgatg     2220

tacatctgcg gggacagcac ggagtgcagc aacctgctgc tgcagtacgg gagcttctgc     2280

acgcagctga accgggcgct gacggggatc gcggtggagc aggacaagaa cacgcaggag     2340

gtgttcgcgc aggtgaagca gatctacaag acgccgccga tcaaggactt cggggggttc     2400

aacttcagcc agatcctgcc ggacccgagc aagccgagca agcggagctt catcgaggac     2460

ctgctgttca acaaggtgac gctggcggac gcggggttca tcaagcagta cggggactgc     2520

ctgggggaca tcgcggcgcg ggacctgatc tgcgcgcaga agttcaacgg gctgacggtg     2580

ctgccgccgc tgctgacgga cgagatgatc gcgcagtaca cgagcgcgct gctggcgggg     2640

acgatcacga gcgggtggac gttcggggcg ggggcggcgc tgcagatccc gttcgcgatg     2700

cagatggcgt accggttcaa cgggatcggg gtgacgcaga acgtgctgta cgagaaccag     2760

aagctgatcg cgaaccagtt caacagcgcg atcgggaaga tccaggacag cctgagcagc     2820

acggcgagcg cgctggggaa gctgcaggac gtggtgaacc agaacgcgca ggcgctgaac     2880

acgctggtga agcagctgag cagcaacttc ggggcgatca gcagcgtgct gaacgacatc     2940

ctgagccggc tggacaaggt ggaggcggag gtgcagatcg accggctgat cacggggcgg     3000

ctgcagagcc tgcagacgta cgtgacgcag cagctgatcc gggcggcgga gatccgggcg     3060

agcgcgaacc tggcggcgac gaagatgagc gagtgcgtgc tggggcagag caagcgggtg     3120

gacttctgcg ggaaggggta ccacctgatg agcttcccgc agagcgcgcc gcacggggtg     3180

gtgttcctgc acgtgacgta cgtgccggcg caggagaaga acttcacgac ggcgccggcg     3240

atctgccacg acgggaaggc gcacttcccg cgggaggggg tgttcgtgag caacgggacg     3300

cactggttcg tgacgcagcg gaacttctac gagccgcaga tcatcacgac ggacaacacg     3360

ttcgtgagcg ggaactgcga cgtggtgatc gggatcgtga acaacacggt gtacgacccg     3420

ctgcagccgg agctggacag cttcaaggag gagctggaca agtacttcaa gaaccacacg     3480

agcccggacg tggacctggg ggacatcagc gggatcaacg cgagcgtggt gaacatccag     3540

aaggagatcg accggctgaa cgaggtggcg aagaacctga acgagagcct gatcgacctg     3600

caggagctgg ggaagtacga gcagtacatc aagtggccgt ggtacatctg gctggggttc     3660

atcgcggggc tgatcgcgat cgtgatggtg acgatcatgc tgtgctgcat gacgagctgc     3720

tgcagctgcc tgaaggggtg ctgcagctgc gggagctgct gcaagttcga cgaggacgac     3780

agcgagccgg tgctgaaggg ggtgaagctg cactacacgt ga                        3822


<210>  2
<211>  3822
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Full length ORF (RNA) encoding SARS-CoV2 spike protein (from 
       Esp3I linearized template)

<400>  2
auguucgugu uccuggugcu gcugccgcug gugagcagcc agugcgugaa ccugacgacg       60

cggacgcagc ugccgccggc guacacgaac agcuucacgc ggggggugua cuacccggac      120

aagguguucc ggagcagcgu gcugcacagc acgcaggacc uguuccugcc guucuucagc      180

aacgugacgu gguuccacgc gauccacgug agcgggacga acgggacgaa gcgguucgac      240

aacccggugc ugccguucaa cgacggggug uacuucgcga gcacggagaa gagcaacauc      300

auccgggggu ggaucuucgg gacgacgcug gacagcaaga cgcagagccu gcugaucgug      360

aacaacgcga cgaacguggu gaucaaggug ugcgaguucc aguucugcaa cgacccguuc      420

cugggggugu acuaccacaa gaacaacaag agcuggaugg agagcgaguu ccggguguac      480

agcagcgcga acaacugcac guucgaguac gugagccagc cguuccugau ggaccuggag      540

gggaagcagg ggaacuucaa gaaccugcgg gaguucgugu ucaagaacau cgacggguac      600

uucaagaucu acagcaagca cacgccgauc aaccuggugc gggaccugcc gcagggguuc      660

agcgcgcugg agccgcuggu ggaccugccg aucgggauca acaucacgcg guuccagacg      720

cugcuggcgc ugcaccggag cuaccugacg ccgggggaca gcagcagcgg guggacggcg      780

ggggcggcgg cguacuacgu gggguaccug cagccgcgga cguuccugcu gaaguacaac      840

gagaacggga cgaucacgga cgcgguggac ugcgcgcugg acccgcugag cgagaccaag      900

ugcacgcuga agagcuucac gguggagaag gggaucuacc agacgagcaa cuuccgggug      960

cagccgacgg agagcaucgu gcgguucccg aacaucacga accugugccc guucggggag     1020

guguucaacg cgacgcgguu cgcgagcgug uacgcgugga accggaagcg gaucagcaac     1080

ugcguggcgg acuacagcgu gcuguacaac agcgcgagcu ucagcacguu caagugcuac     1140

ggggugagcc cgacgaagcu gaacgaccug ugcuucacga acguguacgc ggacagcuuc     1200

gugauccggg gggacgaggu gcggcagauc gcgccggggc agacggggaa gaucgcggac     1260

uacaacuaca agcugccgga cgacuucacg gggugcguga ucgcguggaa cagcaacaac     1320

cuggacagca aggugggggg gaacuacaac uaccuguacc ggcuguuccg gaagagcaac     1380

cugaagccgu ucgagcggga caucagcacg gagaucuacc aggcggggag cacgccgugc     1440

aacggggugg agggguucaa cugcuacuuc ccgcugcaga gcuacggguu ccagccgacg     1500

aacggggugg gguaccagcc guaccgggug guggugcuga gcuucgagcu gcugcacgcg     1560

ccggcgacgg ugugcgggcc gaagaagagc acgaaccugg ugaagaacaa gugcgugaac     1620

uucaacuuca acgggcugac ggggacgggg gugcugacgg agagcaacaa gaaguuccug     1680

ccguuccagc aguucgggcg ggacaucgcg gacacgacgg acgcggugcg ggacccgcag     1740

acgcuggaga uccuggacau cacgccgugc agcuucgggg gggugagcgu gaucacgccg     1800

gggacgaaca cgagcaacca gguggcggug cuguaccagg acgugaacug cacggaggug     1860

ccgguggcga uccacgcgga ccagcugacg ccgacguggc ggguguacag cacggggagc     1920

aacguguucc agacgcgggc ggggugccug aucggggcgg agcacgugaa caacagcuac     1980

gagugcgaca ucccgaucgg ggcggggauc ugcgcgagcu accagacgca gacgaacagc     2040

ccgcggcggg cgcggagcgu ggcgagccag agcaucaucg cguacacgau gagccugggg     2100

gcggagaaca gcguggcgua cagcaacaac agcaucgcga ucccgacgaa cuucacgauc     2160

agcgugacga cggagauccu gccggugagc augacgaaga cgagcgugga cugcacgaug     2220

uacaucugcg gggacagcac ggagugcagc aaccugcugc ugcaguacgg gagcuucugc     2280

acgcagcuga accgggcgcu gacggggauc gcgguggagc aggacaagaa cacgcaggag     2340

guguucgcgc aggugaagca gaucuacaag acgccgccga ucaaggacuu cgggggguuc     2400

aacuucagcc agauccugcc ggacccgagc aagccgagca agcggagcuu caucgaggac     2460

cugcuguuca acaaggugac gcuggcggac gcgggguuca ucaagcagua cggggacugc     2520

cugggggaca ucgcggcgcg ggaccugauc ugcgcgcaga aguucaacgg gcugacggug     2580

cugccgccgc ugcugacgga cgagaugauc gcgcaguaca cgagcgcgcu gcuggcgggg     2640

acgaucacga gcggguggac guucggggcg ggggcggcgc ugcagauccc guucgcgaug     2700

cagauggcgu accgguucaa cgggaucggg gugacgcaga acgugcugua cgagaaccag     2760

aagcugaucg cgaaccaguu caacagcgcg aucgggaaga uccaggacag ccugagcagc     2820

acggcgagcg cgcuggggaa gcugcaggac guggugaacc agaacgcgca ggcgcugaac     2880

acgcugguga agcagcugag cagcaacuuc ggggcgauca gcagcgugcu gaacgacauc     2940

cugagccggc uggacaaggu ggaggcggag gugcagaucg accggcugau cacggggcgg     3000

cugcagagcc ugcagacgua cgugacgcag cagcugaucc gggcggcgga gauccgggcg     3060

agcgcgaacc uggcggcgac gaagaugagc gagugcgugc uggggcagag caagcgggug     3120

gacuucugcg ggaaggggua ccaccugaug agcuucccgc agagcgcgcc gcacggggug     3180

guguuccugc acgugacgua cgugccggcg caggagaaga acuucacgac ggcgccggcg     3240

aucugccacg acgggaaggc gcacuucccg cgggaggggg uguucgugag caacgggacg     3300

cacugguucg ugacgcagcg gaacuucuac gagccgcaga ucaucacgac ggacaacacg     3360

uucgugagcg ggaacugcga cguggugauc gggaucguga acaacacggu guacgacccg     3420

cugcagccgg agcuggacag cuucaaggag gagcuggaca aguacuucaa gaaccacacg     3480

agcccggacg uggaccuggg ggacaucagc gggaucaacg cgagcguggu gaacauccag     3540

aaggagaucg accggcugaa cgagguggcg aagaaccuga acgagagccu gaucgaccug     3600

caggagcugg ggaaguacga gcaguacauc aaguggccgu gguacaucug gcugggguuc     3660

aucgcggggc ugaucgcgau cgugauggug acgaucaugc ugugcugcau gacgagcugc     3720

ugcagcugcc ugaaggggug cugcagcugc gggagcugcu gcaaguucga cgaggacgac     3780

agcgagccgg ugcugaaggg ggugaagcug cacuacacgu ga                        3822


<210>  3
<211>  3822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Full length ORF (DNA template) encoding SARS-CoV2 spike protein 
       (BspQI linearized template)

<400>  3
atgttcgtgt tcctggtgct gctgccgctg gtgagcagcc agtgcgtgaa cctgacgacg       60

cggacgcagc tgccgccggc gtacacgaac agcttcacgc ggggggtgta ctacccggac      120

aaggtgttcc ggagcagcgt gctgcacagc acgcaggacc tgttcctgcc gttcttcagc      180

aacgtgacgt ggttccacgc gatccacgtg agcgggacga acgggacgaa gcggttcgac      240

aacccggtgc tgccgttcaa cgacggggtg tacttcgcga gcacggagaa gagtaacatc      300

atccgggggt ggatcttcgg gacgacgctg gacagcaaga cgcagagcct gctgatcgtg      360

aacaacgcga cgaacgtggt gatcaaggtg tgcgagttcc agttctgcaa cgacccgttc      420

ctgggggtgt actaccacaa gaacaacaag agctggatgg agagcgagtt ccgggtgtac      480

agcagcgcga acaactgcac gttcgagtac gtgagccagc cgttcctgat ggacctggag      540

gggaagcagg ggaacttcaa gaacctgcgg gagttcgtgt tcaagaacat cgacgggtac      600

ttcaagatct acagcaagca cacgccgatc aacctggtgc gggacctgcc gcaggggttc      660

agcgcgctgg agccgctggt ggacctgccg atcgggatca acatcacgcg gttccagacg      720

ctgctggcgc tgcaccggag ctacctgacg ccgggggaca gcagcagcgg gtggacggcg      780

ggggcggcgg cgtactacgt ggggtacctg cagccgcgga cgttcctgct gaagtacaac      840

gagaacggga cgatcacgga cgcggtggac tgcgcgctgg acccgctgag cgagaccaag      900

tgcacgctga agagtttcac ggtggagaag gggatctacc agacgagcaa cttccgggtg      960

cagccgacgg agagcatcgt gcggttcccg aacatcacga acctgtgccc gttcggggag     1020

gtgttcaacg cgacgcggtt cgcgagcgtg tacgcgtgga accggaagcg gatcagcaac     1080

tgcgtggcgg actacagcgt gctgtacaac agcgcgagct tcagcacgtt caagtgctac     1140

ggggtgagcc cgacgaagct gaacgacctg tgcttcacga acgtgtacgc ggacagcttc     1200

gtgatccggg gggacgaggt gcggcagatc gcgccggggc agacggggaa gatcgcggac     1260

tacaactaca agctgccgga cgacttcacg gggtgcgtga tcgcgtggaa cagcaacaac     1320

ctggacagca aggtgggggg gaactacaac tacctgtacc ggctgttccg gaagagtaac     1380

ctgaagccgt tcgagcggga catcagcacg gagatctacc aggcggggag cacgccgtgc     1440

aacggggtgg aggggttcaa ctgctacttc ccgctgcaga gctacgggtt ccagccgacg     1500

aacggggtgg ggtaccagcc gtaccgggtg gtggtgctga gcttcgagct gctgcacgcg     1560

ccggcgacgg tgtgcgggcc gaagaagagt acgaacctgg tgaagaacaa gtgcgtgaac     1620

ttcaacttca acgggctgac ggggacgggg gtgctgacgg agagcaacaa gaagttcctg     1680

ccgttccagc agttcgggcg ggacatcgcg gacacgacgg acgcggtgcg ggacccgcag     1740

acgctggaga tcctggacat cacgccgtgc agcttcgggg gggtgagcgt gatcacgccg     1800

gggacgaaca cgagcaacca ggtggcggtg ctgtaccagg acgtgaactg cacggaggtg     1860

ccggtggcga tccacgcgga ccagctgacg ccgacgtggc gggtgtacag cacggggagc     1920

aacgtgttcc agacgcgggc ggggtgcctg atcggggcgg agcacgtgaa caacagctac     1980

gagtgcgaca tcccgatcgg ggcggggatc tgcgcgagct accagacgca gacgaacagc     2040

ccgcggcggg cgcggagcgt ggcgagccag agcatcatcg cgtacacgat gagcctgggg     2100

gcggagaaca gcgtggcgta cagcaacaac agcatcgcga tcccgacgaa cttcacgatc     2160

agcgtgacga cggagatcct gccggtgagc atgacgaaga cgagcgtgga ctgcacgatg     2220

tacatctgcg gggacagcac ggagtgcagc aacctgctgc tgcagtacgg gagcttctgc     2280

acgcagctga accgggcgct gacggggatc gcggtggagc aggacaagaa cacgcaggag     2340

gtgttcgcgc aggtgaagca gatctacaag acgccgccga tcaaggactt cggggggttc     2400

aacttcagcc agatcctgcc ggacccgagc aagccgagca agcggagctt catcgaggac     2460

ctgctgttca acaaggtgac gctggcggac gcggggttca tcaagcagta cggggactgc     2520

ctgggggaca tcgcggcgcg ggacctgatc tgcgcgcaga agttcaacgg gctgacggtg     2580

ctgccgccgc tgctgacgga cgagatgatc gcgcagtaca cgagcgcgct gctggcgggg     2640

acgatcacga gcgggtggac gttcggggcg ggggcggcgc tgcagatccc gttcgcgatg     2700

cagatggcgt accggttcaa cgggatcggg gtgacgcaga acgtgctgta cgagaaccag     2760

aagctgatcg cgaaccagtt caacagcgcg atcgggaaga tccaggacag cctgagcagc     2820

acggcgagcg cgctggggaa gctgcaggac gtggtgaacc agaacgcgca ggcgctgaac     2880

acgctggtga agcagctgag cagcaacttc ggggcgatca gcagcgtgct gaacgacatc     2940

ctgagccggc tggacaaggt ggaggcggag gtgcagatcg accggctgat cacggggcgg     3000

ctgcagagcc tgcagacgta cgtgacgcag cagctgatcc gggcggcgga gatccgggcg     3060

agcgcgaacc tggcggcgac gaagatgagc gagtgcgtgc tggggcagag caagcgggtg     3120

gacttctgcg ggaaggggta ccacctgatg agcttcccgc agagcgcgcc gcacggggtg     3180

gtgttcctgc acgtgacgta cgtgccggcg caggagaaga acttcacgac ggcgccggcg     3240

atctgccacg acgggaaggc gcacttcccg cgggaggggg tgttcgtgag caacgggacg     3300

cactggttcg tgacgcagcg gaacttctac gagccgcaga tcatcacgac ggacaacacg     3360

ttcgtgagcg ggaactgcga cgtggtgatc gggatcgtga acaacacggt gtacgacccg     3420

ctgcagccgg agctggacag cttcaaggag gagctggaca agtacttcaa gaaccacacg     3480

agcccggacg tggacctggg ggacatcagc gggatcaacg cgagcgtggt gaacatccag     3540

aaggagatcg accggctgaa cgaggtggcg aagaacctga acgagagcct gatcgacctg     3600

caggagctgg ggaagtacga gcagtacatc aagtggccgt ggtacatctg gctggggttc     3660

atcgcggggc tgatcgcgat cgtgatggtg acgatcatgc tgtgctgcat gacgagctgc     3720

tgcagctgcc tgaaggggtg ctgcagctgc gggagctgct gcaagttcga cgaggacgac     3780

agcgagccgg tgctgaaggg ggtgaagctg cactacacgt ga                        3822


<210>  4
<211>  3822
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Full length ORF (RNA) encoding SARS-CoV2 spike protein (BspQI 
       linearized template)

<400>  4
auguucgugu uccuggugcu gcugccgcug gugagcagcc agugcgugaa ccugacgacg       60

cggacgcagc ugccgccggc guacacgaac agcuucacgc ggggggugua cuacccggac      120

aagguguucc ggagcagcgu gcugcacagc acgcaggacc uguuccugcc guucuucagc      180

aacgugacgu gguuccacgc gauccacgug agcgggacga acgggacgaa gcgguucgac      240

aacccggugc ugccguucaa cgacggggug uacuucgcga gcacggagaa gaguaacauc      300

auccgggggu ggaucuucgg gacgacgcug gacagcaaga cgcagagccu gcugaucgug      360

aacaacgcga cgaacguggu gaucaaggug ugcgaguucc aguucugcaa cgacccguuc      420

cugggggugu acuaccacaa gaacaacaag agcuggaugg agagcgaguu ccggguguac      480

agcagcgcga acaacugcac guucgaguac gugagccagc cguuccugau ggaccuggag      540

gggaagcagg ggaacuucaa gaaccugcgg gaguucgugu ucaagaacau cgacggguac      600

uucaagaucu acagcaagca cacgccgauc aaccuggugc gggaccugcc gcagggguuc      660

agcgcgcugg agccgcuggu ggaccugccg aucgggauca acaucacgcg guuccagacg      720

cugcuggcgc ugcaccggag cuaccugacg ccgggggaca gcagcagcgg guggacggcg      780

ggggcggcgg cguacuacgu gggguaccug cagccgcgga cguuccugcu gaaguacaac      840

gagaacggga cgaucacgga cgcgguggac ugcgcgcugg acccgcugag cgagaccaag      900

ugcacgcuga agaguuucac gguggagaag gggaucuacc agacgagcaa cuuccgggug      960

cagccgacgg agagcaucgu gcgguucccg aacaucacga accugugccc guucggggag     1020

guguucaacg cgacgcgguu cgcgagcgug uacgcgugga accggaagcg gaucagcaac     1080

ugcguggcgg acuacagcgu gcuguacaac agcgcgagcu ucagcacguu caagugcuac     1140

ggggugagcc cgacgaagcu gaacgaccug ugcuucacga acguguacgc ggacagcuuc     1200

gugauccggg gggacgaggu gcggcagauc gcgccggggc agacggggaa gaucgcggac     1260

uacaacuaca agcugccgga cgacuucacg gggugcguga ucgcguggaa cagcaacaac     1320

cuggacagca aggugggggg gaacuacaac uaccuguacc ggcuguuccg gaagaguaac     1380

cugaagccgu ucgagcggga caucagcacg gagaucuacc aggcggggag cacgccgugc     1440

aacggggugg agggguucaa cugcuacuuc ccgcugcaga gcuacggguu ccagccgacg     1500

aacggggugg gguaccagcc guaccgggug guggugcuga gcuucgagcu gcugcacgcg     1560

ccggcgacgg ugugcgggcc gaagaagagu acgaaccugg ugaagaacaa gugcgugaac     1620

uucaacuuca acgggcugac ggggacgggg gugcugacgg agagcaacaa gaaguuccug     1680

ccguuccagc aguucgggcg ggacaucgcg gacacgacgg acgcggugcg ggacccgcag     1740

acgcuggaga uccuggacau cacgccgugc agcuucgggg gggugagcgu gaucacgccg     1800

gggacgaaca cgagcaacca gguggcggug cuguaccagg acgugaacug cacggaggug     1860

ccgguggcga uccacgcgga ccagcugacg ccgacguggc ggguguacag cacggggagc     1920

aacguguucc agacgcgggc ggggugccug aucggggcgg agcacgugaa caacagcuac     1980

gagugcgaca ucccgaucgg ggcggggauc ugcgcgagcu accagacgca gacgaacagc     2040

ccgcggcggg cgcggagcgu ggcgagccag agcaucaucg cguacacgau gagccugggg     2100

gcggagaaca gcguggcgua cagcaacaac agcaucgcga ucccgacgaa cuucacgauc     2160

agcgugacga cggagauccu gccggugagc augacgaaga cgagcgugga cugcacgaug     2220

uacaucugcg gggacagcac ggagugcagc aaccugcugc ugcaguacgg gagcuucugc     2280

acgcagcuga accgggcgcu gacggggauc gcgguggagc aggacaagaa cacgcaggag     2340

guguucgcgc aggugaagca gaucuacaag acgccgccga ucaaggacuu cgggggguuc     2400

aacuucagcc agauccugcc ggacccgagc aagccgagca agcggagcuu caucgaggac     2460

cugcuguuca acaaggugac gcuggcggac gcgggguuca ucaagcagua cggggacugc     2520

cugggggaca ucgcggcgcg ggaccugauc ugcgcgcaga aguucaacgg gcugacggug     2580

cugccgccgc ugcugacgga cgagaugauc gcgcaguaca cgagcgcgcu gcuggcgggg     2640

acgaucacga gcggguggac guucggggcg ggggcggcgc ugcagauccc guucgcgaug     2700

cagauggcgu accgguucaa cgggaucggg gugacgcaga acgugcugua cgagaaccag     2760

aagcugaucg cgaaccaguu caacagcgcg aucgggaaga uccaggacag ccugagcagc     2820

acggcgagcg cgcuggggaa gcugcaggac guggugaacc agaacgcgca ggcgcugaac     2880

acgcugguga agcagcugag cagcaacuuc ggggcgauca gcagcgugcu gaacgacauc     2940

cugagccggc uggacaaggu ggaggcggag gugcagaucg accggcugau cacggggcgg     3000

cugcagagcc ugcagacgua cgugacgcag cagcugaucc gggcggcgga gauccgggcg     3060

agcgcgaacc uggcggcgac gaagaugagc gagugcgugc uggggcagag caagcgggug     3120

gacuucugcg ggaaggggua ccaccugaug agcuucccgc agagcgcgcc gcacggggug     3180

guguuccugc acgugacgua cgugccggcg caggagaaga acuucacgac ggcgccggcg     3240

aucugccacg acgggaaggc gcacuucccg cgggaggggg uguucgugag caacgggacg     3300

cacugguucg ugacgcagcg gaacuucuac gagccgcaga ucaucacgac ggacaacacg     3360

uucgugagcg ggaacugcga cguggugauc gggaucguga acaacacggu guacgacccg     3420

cugcagccgg agcuggacag cuucaaggag gagcuggaca aguacuucaa gaaccacacg     3480

agcccggacg uggaccuggg ggacaucagc gggaucaacg cgagcguggu gaacauccag     3540

aaggagaucg accggcugaa cgagguggcg aagaaccuga acgagagccu gaucgaccug     3600

caggagcugg ggaaguacga gcaguacauc aaguggccgu gguacaucug gcugggguuc     3660

aucgcggggc ugaucgcgau cgugauggug acgaucaugc ugugcugcau gacgagcugc     3720

ugcagcugcc ugaaggggug cugcagcugc gggagcugcu gcaaguucga cgaggacgac     3780

agcgagccgg ugcugaaggg ggugaagcug cacuacacgu ga                        3822


<210>  5
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SARS-CoV2 spike protein amino acid sequence (GenBank: QHD43416.1)

<400>  5

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  6
<211>  4123
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  mRNA sequence encoding SARS-CoV2 spike protein (Esp3I linearized 
       template), and including non-coding regions (ITS, 5' UTR, Kozak 
       Sequence, 3' UTR)

<400>  6
gaggagacca ggaauuacau uugcuucuga cacaacugug uucacuagca accucaaaca       60

gagccgccau guucguguuc cuggugcugc ugccgcuggu gagcagccag ugcgugaacc      120

ugacgacgcg gacgcagcug ccgccggcgu acacgaacag cuucacgcgg gggguguacu      180

acccggacaa gguguuccgg agcagcgugc ugcacagcac gcaggaccug uuccugccgu      240

ucuucagcaa cgugacgugg uuccacgcga uccacgugag cgggacgaac gggacgaagc      300

gguucgacaa cccggugcug ccguucaacg acggggugua cuucgcgagc acggagaaga      360

gcaacaucau ccgggggugg aucuucggga cgacgcugga cagcaagacg cagagccugc      420

ugaucgugaa caacgcgacg aacgugguga ucaaggugug cgaguuccag uucugcaacg      480

acccguuccu ggggguguac uaccacaaga acaacaagag cuggauggag agcgaguucc      540

ggguguacag cagcgcgaac aacugcacgu ucgaguacgu gagccagccg uuccugaugg      600

accuggaggg gaagcagggg aacuucaaga accugcggga guucguguuc aagaacaucg      660

acggguacuu caagaucuac agcaagcaca cgccgaucaa ccuggugcgg gaccugccgc      720

agggguucag cgcgcuggag ccgcuggugg accugccgau cgggaucaac aucacgcggu      780

uccagacgcu gcuggcgcug caccggagcu accugacgcc gggggacagc agcagcgggu      840

ggacggcggg ggcggcggcg uacuacgugg gguaccugca gccgcggacg uuccugcuga      900

aguacaacga gaacgggacg aucacggacg cgguggacug cgcgcuggac ccgcugagcg      960

agaccaagug cacgcugaag agcuucacgg uggagaaggg gaucuaccag acgagcaacu     1020

uccgggugca gccgacggag agcaucgugc gguucccgaa caucacgaac cugugcccgu     1080

ucggggaggu guucaacgcg acgcgguucg cgagcgugua cgcguggaac cggaagcgga     1140

ucagcaacug cguggcggac uacagcgugc uguacaacag cgcgagcuuc agcacguuca     1200

agugcuacgg ggugagcccg acgaagcuga acgaccugug cuucacgaac guguacgcgg     1260

acagcuucgu gauccggggg gacgaggugc ggcagaucgc gccggggcag acggggaaga     1320

ucgcggacua caacuacaag cugccggacg acuucacggg gugcgugauc gcguggaaca     1380

gcaacaaccu ggacagcaag guggggggga acuacaacua ccuguaccgg cuguuccgga     1440

agagcaaccu gaagccguuc gagcgggaca ucagcacgga gaucuaccag gcggggagca     1500

cgccgugcaa cgggguggag ggguucaacu gcuacuuccc gcugcagagc uacggguucc     1560

agccgacgaa cggggugggg uaccagccgu accggguggu ggugcugagc uucgagcugc     1620

ugcacgcgcc ggcgacggug ugcgggccga agaagagcac gaaccuggug aagaacaagu     1680

gcgugaacuu caacuucaac gggcugacgg ggacgggggu gcugacggag agcaacaaga     1740

aguuccugcc guuccagcag uucgggcggg acaucgcgga cacgacggac gcggugcggg     1800

acccgcagac gcuggagauc cuggacauca cgccgugcag cuucgggggg gugagcguga     1860

ucacgccggg gacgaacacg agcaaccagg uggcggugcu guaccaggac gugaacugca     1920

cggaggugcc gguggcgauc cacgcggacc agcugacgcc gacguggcgg guguacagca     1980

cggggagcaa cguguuccag acgcgggcgg ggugccugau cggggcggag cacgugaaca     2040

acagcuacga gugcgacauc ccgaucgggg cggggaucug cgcgagcuac cagacgcaga     2100

cgaacagccc gcggcgggcg cggagcgugg cgagccagag caucaucgcg uacacgauga     2160

gccugggggc ggagaacagc guggcguaca gcaacaacag caucgcgauc ccgacgaacu     2220

ucacgaucag cgugacgacg gagauccugc cggugagcau gacgaagacg agcguggacu     2280

gcacgaugua caucugcggg gacagcacgg agugcagcaa ccugcugcug caguacggga     2340

gcuucugcac gcagcugaac cgggcgcuga cggggaucgc gguggagcag gacaagaaca     2400

cgcaggaggu guucgcgcag gugaagcaga ucuacaagac gccgccgauc aaggacuucg     2460

ggggguucaa cuucagccag auccugccgg acccgagcaa gccgagcaag cggagcuuca     2520

ucgaggaccu gcuguucaac aaggugacgc uggcggacgc gggguucauc aagcaguacg     2580

gggacugccu gggggacauc gcggcgcggg accugaucug cgcgcagaag uucaacgggc     2640

ugacggugcu gccgccgcug cugacggacg agaugaucgc gcaguacacg agcgcgcugc     2700

uggcggggac gaucacgagc ggguggacgu ucggggcggg ggcggcgcug cagaucccgu     2760

ucgcgaugca gauggcguac cgguucaacg ggaucggggu gacgcagaac gugcuguacg     2820

agaaccagaa gcugaucgcg aaccaguuca acagcgcgau cgggaagauc caggacagcc     2880

ugagcagcac ggcgagcgcg cuggggaagc ugcaggacgu ggugaaccag aacgcgcagg     2940

cgcugaacac gcuggugaag cagcugagca gcaacuucgg ggcgaucagc agcgugcuga     3000

acgacauccu gagccggcug gacaaggugg aggcggaggu gcagaucgac cggcugauca     3060

cggggcggcu gcagagccug cagacguacg ugacgcagca gcugauccgg gcggcggaga     3120

uccgggcgag cgcgaaccug gcggcgacga agaugagcga gugcgugcug gggcagagca     3180

agcgggugga cuucugcggg aagggguacc accugaugag cuucccgcag agcgcgccgc     3240

acgggguggu guuccugcac gugacguacg ugccggcgca ggagaagaac uucacgacgg     3300

cgccggcgau cugccacgac gggaaggcgc acuucccgcg ggagggggug uucgugagca     3360

acgggacgca cugguucgug acgcagcgga acuucuacga gccgcagauc aucacgacgg     3420

acaacacguu cgugagcggg aacugcgacg uggugaucgg gaucgugaac aacacggugu     3480

acgacccgcu gcagccggag cuggacagcu ucaaggagga gcuggacaag uacuucaaga     3540

accacacgag cccggacgug gaccuggggg acaucagcgg gaucaacgcg agcgugguga     3600

acauccagaa ggagaucgac cggcugaacg agguggcgaa gaaccugaac gagagccuga     3660

ucgaccugca ggagcugggg aaguacgagc aguacaucaa guggccgugg uacaucuggc     3720

ugggguucau cgcggggcug aucgcgaucg ugauggugac gaucaugcug ugcugcauga     3780

cgagcugcug cagcugccug aaggggugcu gcagcugcgg gagcugcugc aaguucgacg     3840

aggacgacag cgagccggug cugaaggggg ugaagcugca cuacacguga agcucgcuuu     3900

cuugcugucc aauuucuauu aaagguuccu uuguucccua aguccaacua cuaaacuggg     3960

ggauauuaug aagggccuug agcaucugga uucugccuaa uaagaaacau uuauugucau     4020

ugcaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4080

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa                       4123


<210>  7
<211>  4123
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  mRNA sequence encoding SARS-CoV2 spike protein (BspQI linearized 
       template), and including non-coding regions (ITS, 5' UTR, Kozak 
       sequence, 3' UTR)

<400>  7
gaggagacca ggaauuacau uugcuucuga cacaacugug uucacuagca accucaaaca       60

gagccgccau guucguguuc cuggugcugc ugccgcuggu gagcagccag ugcgugaacc      120

ugacgacgcg gacgcagcug ccgccggcgu acacgaacag cuucacgcgg gggguguacu      180

acccggacaa gguguuccgg agcagcgugc ugcacagcac gcaggaccug uuccugccgu      240

ucuucagcaa cgugacgugg uuccacgcga uccacgugag cgggacgaac gggacgaagc      300

gguucgacaa cccggugcug ccguucaacg acggggugua cuucgcgagc acggagaaga      360

guaacaucau ccgggggugg aucuucggga cgacgcugga cagcaagacg cagagccugc      420

ugaucgugaa caacgcgacg aacgugguga ucaaggugug cgaguuccag uucugcaacg      480

acccguuccu ggggguguac uaccacaaga acaacaagag cuggauggag agcgaguucc      540

ggguguacag cagcgcgaac aacugcacgu ucgaguacgu gagccagccg uuccugaugg      600

accuggaggg gaagcagggg aacuucaaga accugcggga guucguguuc aagaacaucg      660

acggguacuu caagaucuac agcaagcaca cgccgaucaa ccuggugcgg gaccugccgc      720

agggguucag cgcgcuggag ccgcuggugg accugccgau cgggaucaac aucacgcggu      780

uccagacgcu gcuggcgcug caccggagcu accugacgcc gggggacagc agcagcgggu      840

ggacggcggg ggcggcggcg uacuacgugg gguaccugca gccgcggacg uuccugcuga      900

aguacaacga gaacgggacg aucacggacg cgguggacug cgcgcuggac ccgcugagcg      960

agaccaagug cacgcugaag aguuucacgg uggagaaggg gaucuaccag acgagcaacu     1020

uccgggugca gccgacggag agcaucgugc gguucccgaa caucacgaac cugugcccgu     1080

ucggggaggu guucaacgcg acgcgguucg cgagcgugua cgcguggaac cggaagcgga     1140

ucagcaacug cguggcggac uacagcgugc uguacaacag cgcgagcuuc agcacguuca     1200

agugcuacgg ggugagcccg acgaagcuga acgaccugug cuucacgaac guguacgcgg     1260

acagcuucgu gauccggggg gacgaggugc ggcagaucgc gccggggcag acggggaaga     1320

ucgcggacua caacuacaag cugccggacg acuucacggg gugcgugauc gcguggaaca     1380

gcaacaaccu ggacagcaag guggggggga acuacaacua ccuguaccgg cuguuccgga     1440

agaguaaccu gaagccguuc gagcgggaca ucagcacgga gaucuaccag gcggggagca     1500

cgccgugcaa cgggguggag ggguucaacu gcuacuuccc gcugcagagc uacggguucc     1560

agccgacgaa cggggugggg uaccagccgu accggguggu ggugcugagc uucgagcugc     1620

ugcacgcgcc ggcgacggug ugcgggccga agaagaguac gaaccuggug aagaacaagu     1680

gcgugaacuu caacuucaac gggcugacgg ggacgggggu gcugacggag agcaacaaga     1740

aguuccugcc guuccagcag uucgggcggg acaucgcgga cacgacggac gcggugcggg     1800

acccgcagac gcuggagauc cuggacauca cgccgugcag cuucgggggg gugagcguga     1860

ucacgccggg gacgaacacg agcaaccagg uggcggugcu guaccaggac gugaacugca     1920

cggaggugcc gguggcgauc cacgcggacc agcugacgcc gacguggcgg guguacagca     1980

cggggagcaa cguguuccag acgcgggcgg ggugccugau cggggcggag cacgugaaca     2040

acagcuacga gugcgacauc ccgaucgggg cggggaucug cgcgagcuac cagacgcaga     2100

cgaacagccc gcggcgggcg cggagcgugg cgagccagag caucaucgcg uacacgauga     2160

gccugggggc ggagaacagc guggcguaca gcaacaacag caucgcgauc ccgacgaacu     2220

ucacgaucag cgugacgacg gagauccugc cggugagcau gacgaagacg agcguggacu     2280

gcacgaugua caucugcggg gacagcacgg agugcagcaa ccugcugcug caguacggga     2340

gcuucugcac gcagcugaac cgggcgcuga cggggaucgc gguggagcag gacaagaaca     2400

cgcaggaggu guucgcgcag gugaagcaga ucuacaagac gccgccgauc aaggacuucg     2460

ggggguucaa cuucagccag auccugccgg acccgagcaa gccgagcaag cggagcuuca     2520

ucgaggaccu gcuguucaac aaggugacgc uggcggacgc gggguucauc aagcaguacg     2580

gggacugccu gggggacauc gcggcgcggg accugaucug cgcgcagaag uucaacgggc     2640

ugacggugcu gccgccgcug cugacggacg agaugaucgc gcaguacacg agcgcgcugc     2700

uggcggggac gaucacgagc ggguggacgu ucggggcggg ggcggcgcug cagaucccgu     2760

ucgcgaugca gauggcguac cgguucaacg ggaucggggu gacgcagaac gugcuguacg     2820

agaaccagaa gcugaucgcg aaccaguuca acagcgcgau cgggaagauc caggacagcc     2880

ugagcagcac ggcgagcgcg cuggggaagc ugcaggacgu ggugaaccag aacgcgcagg     2940

cgcugaacac gcuggugaag cagcugagca gcaacuucgg ggcgaucagc agcgugcuga     3000

acgacauccu gagccggcug gacaaggugg aggcggaggu gcagaucgac cggcugauca     3060

cggggcggcu gcagagccug cagacguacg ugacgcagca gcugauccgg gcggcggaga     3120

uccgggcgag cgcgaaccug gcggcgacga agaugagcga gugcgugcug gggcagagca     3180

agcgggugga cuucugcggg aagggguacc accugaugag cuucccgcag agcgcgccgc     3240

acgggguggu guuccugcac gugacguacg ugccggcgca ggagaagaac uucacgacgg     3300

cgccggcgau cugccacgac gggaaggcgc acuucccgcg ggagggggug uucgugagca     3360

acgggacgca cugguucgug acgcagcgga acuucuacga gccgcagauc aucacgacgg     3420

acaacacguu cgugagcggg aacugcgacg uggugaucgg gaucgugaac aacacggugu     3480

acgacccgcu gcagccggag cuggacagcu ucaaggagga gcuggacaag uacuucaaga     3540

accacacgag cccggacgug gaccuggggg acaucagcgg gaucaacgcg agcgugguga     3600

acauccagaa ggagaucgac cggcugaacg agguggcgaa gaaccugaac gagagccuga     3660

ucgaccugca ggagcugggg aaguacgagc aguacaucaa guggccgugg uacaucuggc     3720

ugggguucau cgcggggcug aucgcgaucg ugauggugac gaucaugcug ugcugcauga     3780

cgagcugcug cagcugccug aaggggugcu gcagcugcgg gagcugcugc aaguucgacg     3840

aggacgacag cgagccggug cugaaggggg ugaagcugca cuacacguga agcucgcuuu     3900

cuugcugucc aauuucuauu aaagguuccu uuguucccua aguccaacua cuaaacuggg     3960

ggauauuaug aagggccuug agcaucugga uucugccuaa uaagaaacau uuauugucau     4020

ugcaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4080

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa                       4123


<210>  8
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Initial Transcribed Sequence (ITS)

<400>  8
gggaga                                                                   6


<210>  9
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Initial Transcribed Sequence (ITS)

<400>  9
aggaga                                                                   6


<210>  10
<211>  15
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Initial Transcribed Sequence (ITS)

<400>  10
gggagaccag gaauu                                                        15


<210>  11
<211>  15
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Initial Transcribed Sequence (ITS)

<400>  11
aggagaccag gaauu                                                        15


<210>  12
<211>  46
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5' untranslated region (UTR)

<400>  12
acauuugcuu cugacacaac uguguucacu agcaaccuca aacaga                      46


<210>  13
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kozak sequence

<400>  13
gccgcc                                                                   6


<210>  14
<211>  133
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3' untranslated region

<400>  14
agcucgcuuu cuugcugucc aauuucuauu aaagguuccu uuguucccua aguccaacua       60

cuaaacuggg ggauauuaug aagggccuug agcaucugga uucugccuaa uaagaaacau      120

uuauugucau ugc                                                         133


<210>  15
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' poly(A) sequence

<400>  15
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa       60

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa                            100


<210>  16

<400>  16
000

<210>  17
<211>  67
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ITS (SEQ ID: NO: 11) + HBG 5' UTR (SEQ ID NO: 12) + Kozak 
       sequence (SEQ ID NO: 13)

<400>  17
aggagaccag gaauuacauu ugcuucugac acaacugugu ucacuagcaa ccucaaacag       60

agccgcc                                                                 67


<210>  18
<211>  51
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5' HBA sequence

<400>  18
agggcgaacu aguacucuuc ugguccccac agacucagag agaacgccac c                51


<210>  19
<211>  71
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5' HSD sequence

<400>  19
agggucccgc agucggcguc cagcggcucu gcuuguucgu gugugugucg uugcaggccu       60

uauucgccgc c                                                            71


<210>  20
<211>  48
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5' MRNA sequence

<400>  20
agggaaauaa gagagaaaag aagaguaaga agaaauauaa gagccacc                    48


<210>  21
<211>  71
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5' NCA-7d sequence

<400>  21
aggcaaaaau caaaaucaau caucaucaca acaucaacaa ucaaucauca acacaucauc       60

aagacaccac c                                                            71


<210>  22
<211>  133
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3' HBG sequence

<400>  22
agcucgcuuu cuugcugucc aauuucuauu aaagguuccu uuguucccua aguccaacua       60

cuaaacuggg ggauauuaug aagggccuug agcaucugga uucugccuaa uaagaaacau      120

uuauugucau ugc                                                         133


<210>  23
<211>  278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3' AES-mtRNR1 sequence

<400>  23
cugguacugc augcacgcaa ugcuagcugc cccuuucccg uccuggguac cccgagucuc       60

ccccgaccuc gggucccagg uaugcuccca ccuccaccug ccccacucac caccucugcu      120

aguuccagac accucccaag cacgcagcaa ugcagcucaa aacgcuuagc cuagccacac      180

ccccacggga aacagcagug auuaaccuuu agcaauaaac gaaaguuuaa cuaagcuaua      240

cuaaccccag gguuggucaa uuucgugcca gccacacc                              278


<210>  24
<211>  186
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3' ALB sequence

<400>  24
caucacauuu aaaagcaucu cagccuacca ugagaauaag agaaagaaaa ugaagaucaa       60

uagcuuauuc aucucuuuuu cuuuuucguu gguguaaagc caacacccug ucuaaaaaac      120

auaaauuucu uuaaucauuu ugccucuugu cucugugcuc cacucaccaa aaaauggaaa      180

gaaccu                                                                 186


<210>  25
<211>  110
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3' HBA sequence

<400>  25
ugauaauagg cuggagccuc gguggccaug cuucuugccc cuugggccuc cccccagccc       60

cuccuccccu uccugcaccc guacccccgu ggucuuugaa uaaagucuga                 110


<210>  26
<211>  122
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3' S27a+R3U sequence

<400>  26
uuguguaugc guuaauaaaa agaaggaacu cguaaaaacu caauguauuu cugaggaagc       60

guggugcaua augccacgca gcgucugcau aacuuuuauu auuucuuuua uuaaucaaca      120

aa                                                                     122


<210>  27
<211>  67
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5' HBG-ITS sequence

<400>  27
aggagaccag gaauuacauu ugcuucugac acaacugugu ucacuagcaa ccucaaacag       60

agccgcc                                                                 67


<210>  28
<211>  67
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5' HBG-ITS sequence

<400>  28
gggagaccag gaauuacauu ugcuucugac acaacugugu ucacuagcaa ccucaaacag       60

agccgcc                                                                 67


<210>  29
<211>  55
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5' HBG sequence

<400>  29
aggacauuug cuucugacac aacuguguuc acuagcaacc ucaaacagag ccgcc            55


<210>  30
<211>  53
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5' HBG sequence

<400>  30
gacauuugcu ucugacacaa cuguguucac uagcaaccuc aaacagagcc gcc              53


<210>  31
<211>  85
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5' NCA-7d-ITS sequence

<400>  31
aggagaccag gaauuggcaa aaaucaaaau caaucaucau cacaacauca acaaucaauc       60

aucaacacau caucaagaca ccacc                                             85


<210>  32
<211>  85
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5' NCA-7d-ITS sequence

<400>  32
gggagaccag gaauuggcaa aaaucaaaau caaucaucau cacaacauca acaaucaauc       60

aucaacacau caucaagaca ccacc                                             85


<210>  33
<211>  71
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5' NCA-7d sequence

<400>  33
aggcaaaaau caaaaucaau caucaucaca acaucaacaa ucaaucauca acacaucauc       60

aagacaccac c                                                            71


<210>  34
<211>  69
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5' NCA-7d sequence

<400>  34
ggcaaaaauc aaaaucaauc aucaucacaa caucaacaau caaucaucaa cacaucauca       60

agacaccac                                                               69


<210>  35
<211>  68
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5' NCA-7d sequence

<400>  35
gcaaaaauca aaaucaauca ucaucacaac aucaacaauc aaucaucaac acaucaucaa       60

gacaccac                                                                68


