                         SEQUENCE LISTING

<110>  Salk Institute for Biological Studies
 
<120>  MULTIPLEX CRISPR/Cas9-MEDIATED TARGET GENE ACTIVATION SYSTEM

<130>  7158-105630-02

<150>  US 63/181,059
<151>  2021-04-28

<160>  61    

<170>  PatentIn version 3.5

<210>  1
<211>  444
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding multiplex crRNAs

<400>  1
gaaccattca aaacagcata gcaagttaaa ataaggctag tccgttatca acttgaaaaa       60

gtggcaccga gtcggtgcgg gagcggccag catgaggatc acccatgcct gcagggccgc      120

cacgagcggg gccaacatga ggatcaccca tgtctgcagg gccccgctcg tgttcccaac      180

aaagcaccag tggtctagtg gtagaatagt accctgccac ggtacagacc cgggttcgat      240

tcccggctgg tgcagagagc agcagttggt tttagagcta tgctgttttg ggccggcatg      300

gtcccagcct cctcgctggc gccggctggg caacatgctt cggcatggcg aatgggacat      360

tcaactgatg agtccgtgag gacgaaacga gtaagctcgt cttgaataaa gggcagtttt      420

agagctatgc tgttttgttt tttt                                             444


<210>  2
<211>  1243
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding multiplex crRNAs with a dgRNA

<400>  2
aaaaaaagca ccagccggga atcgaacccg ggtctgtacc gtggcagggt actattctac       60

cactagacca ctggtgcttt gttgcaccga ctcggtgcca cttggccctg caggcatggg      120

tgatcctcat gctggccaag ttgataacgg actagcctta tttcaacttg ctaggccctg      180

caggcatggg tgatcctcat gctggcctag ctctgaaacg tcgtgcgtgc tggcaaacaa      240

ggcttttctc caagggatat ttatagtctc aaaacacaca attactttac agttagggtg      300

agtttccttt tgtgctgttt tttaaaataa taatttagta tttgtatctc ttatagaaat      360

ccaagcctat catgtaaaat gtagctagta ttaaaaagaa cagattatct gtcttttatc      420

gcacattaag cctctatagt tactaggaaa tattatatgc aaattaaccg gggcagggga      480

gtagccgagc ttctcccaca agtctgtgcg agggggccgg cgcgggccta gagatggcgg      540

cgtcggatcg agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct      600

gttagagaga taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg      660

tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg      720

gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg      780

tggaaaggac gaaacaccgg aaccattcaa aacagcatag caagttaaaa taaggctagt      840

ccgttatcaa cttgaaaaag tggcaccgag tcggtgcggg agcggccagc atgaggatca      900

cccatgcctg cagggccgcc acgagcgggg ccaacatgag gatcacccat gtctgcaggg      960

ccccgctcgt gttcccaaca aagcaccagt ggtctagtgg tagaatagta ccctgccacg     1020

gtacagaccc gggttcgatt cccggctggt gcagagagca gcagttggtt ttagagctat     1080

gctgttttgg gccggcatgg tcccagcctc ctcgctggcg ccggctgggc aacatgcttc     1140

ggcatggcga atgggacatt caactgatga gtccgtgagg acgaaacgag taagctcgtc     1200

ttgaataaag ggcagtttta gagctatgct gttttgtttt ttt                       1243


<210>  3
<211>  956
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding multiplex sgRNAs

<400>  3
aaaaaaagca ccagccggga atcgaacccg ggtctgtacc gtggcagggt actattctac       60

cactagacca ctggtgcttt gttgcaccga ctcggtgcca cttggccctg caggcatggg      120

tgatcctcat gctggccaag ttgataacgg actagcctta tttcaacttg ctaggccctg      180

caggcatggg tgatcctcat gctggcctag ctctgaaacg tcgtgcgtgc tggcaaacaa      240

ggcttttctc caagggatat ttatagtctc aaaacacaca attactttac agttagggtg      300

agtttccttt tgtgctgttt tttaaaataa taatttagta tttgtatctc ttatagaaat      360

ccaagcctat catgtaaaat gtagctagta ttaaaaagaa cagattatct gtcttttatc      420

gcacattaag cctctatagt tactaggaaa tattatatgc aaattaaccg gggcagggga      480

gtagccgagc ttctcccaca agtctgtgcg agggggccgg cgcgggccta gagatggcgg      540

cgtcggatcg agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct      600

gttagagaga taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg      660

tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg      720

gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg      780

tggaaaggac gaaacaccgc aaagcggcag gaggtttcag agctaggcca gcatgaggat      840

cacccatgcc tgcagggcct agcaagttga aataaggcta gtccgttatc aacttggcca      900

gcatgaggat cacccatgcc tgcagggcca agtggcaccg agtcggtgct tttttt          956


<210>  4
<211>  1195
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding multiplexed sgRNAs

<400>  4
aaaaaaagca ccagccggga atcgaacccg ggtctgtacc gtggcagggt actattctac       60

cactagacca ctggtgcttt gttgcaccga ctcggtgcca cttggccctg caggcatggg      120

tgatcctcat gctggccaag ttgataacgg actagcctta tttcaacttg ctaggccctg      180

caggcatggg tgatcctcat gctggcctag ctctgaaacg tcgtgcgtgc tggcaaacaa      240

ggcttttctc caagggatat ttatagtctc aaaacacaca attactttac agttagggtg      300

agtttccttt tgtgctgttt tttaaaataa taatttagta tttgtatctc ttatagaaat      360

ccaagcctat catgtaaaat gtagctagta ttaaaaagaa cagattatct gtcttttatc      420

gcacattaag cctctatagt tactaggaaa tattatatgc aaattaaccg gggcagggga      480

gtagccgagc ttctcccaca agtctgtgcg agggggccgg cgcgggccta gagatggcgg      540

cgtcggatcg agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct      600

gttagagaga taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg      660

tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg      720

gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg      780

tggaaaggac gaaacaccgg acaatttgaa taaagggcag tttcagagct aggccagcat      840

gaggatcacc catgcctgca gggcctagca agttgaaata aggctagtcc gttatcaact      900

tggccagcat gaggatcacc catgcctgca gggccaagtg gcaccgagtc ggtgcaacaa      960

agcgcaagtg gtttagtggt aaaatccaac gttgccatcg ttgggccccc ggttcgattc     1020

cgggcttgcg caaaggtaga gagcagcagt tggtttcaga gctaggccag catgaggatc     1080

acccatgcct gcagggccta gcaagttgaa ataaggctag tccgttatca acttggccag     1140

catgaggatc acccatgcct gcagggccaa gtggcaccga gtcggtgctt ttttt          1195


<210>  5
<211>  1183
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding multiplex sgRNAs

<400>  5
aaaaaaagca ccagccggga atcgaacccg ggtctgtacc gtggcagggt actattctac       60

cactagacca ctggtgcttt gttgcaccga ctcggtgcca cttggccctg caggcatggg      120

tgatcctcat gctggccaag ttgataacgg actagcctta tttcaacttg ctaggccctg      180

caggcatggg tgatcctcat gctggcctag ctctgaaacg tcgtgcgtgc tggcaaacaa      240

ggcttttctc caagggatat ttatagtctc aaaacacaca attactttac agttagggtg      300

agtttccttt tgtgctgttt tttaaaataa taatttagta tttgtatctc ttatagaaat      360

ccaagcctat catgtaaaat gtagctagta ttaaaaagaa cagattatct gtcttttatc      420

gcacattaag cctctatagt tactaggaaa tattatatgc aaattaaccg gggcagggga      480

gtagccgagc ttctcccaca agtctgtgcg agggggccgg cgcgggccta gagatggcgg      540

cgtcggatcg agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct      600

gttagagaga taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg      660

tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg      720

gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg      780

tggaaaggac gaaacaccgt gcccctcctt tccgtttcag agctaggcca gcatgaggat      840

cacccatgcc tgcagggcct agcaagttga aataaggcta gtccgttatc aacttggcca      900

gcatgaggat cacccatgcc tgcagggcca agtggcaccg agtcggtgca acaaagcgca      960

agtggtttag tggtaaaatc caacgttgcc atcgttgggc ccccggttcg attccgggct     1020

tgcgcacaaa gcggcaggag gtttcagagc taggccagca tgaggatcac ccatgcctgc     1080

agggcctagc aagttgaaat aaggctagtc cgttatcaac ttggccagca tgaggatcac     1140

ccatgcctgc agggccaagt ggcaccgagt cggtgctttt ttt                       1183


<210>  6
<211>  1334
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding multiplex sgRNAs

<400>  6
aaaaaaagca ccgactcggt gccacttggc cctgcaggca tgggtgatcc tcatgctggc       60

caagttgata acggactagc cttatttcaa cttgctaggc cctgcaggca tgggtgatcc      120

tcatgctggc ctagctctga aactgccctt tattcaatgc accagccggg aatcgaaccc      180

gggtctgtac cgtggcaggg tactattcta ccactagacc actggtgctt tgttgcaccg      240

actcggtgcc acttggccct gcaggcatgg gtgatcctca tgctggccaa gttgataacg      300

gactagcctt atttcaactt gctaggccct gcaggcatgg gtgatcctca tgctggccta      360

gctctgaaac gtcgtgcgtg ctggcaaaca aggcttttct ccaagggata tttatagtct      420

caaaacacac aattacttta cagttagggt gagtttcctt ttgtgctgtt ttttaaaata      480

ataatttagt atttgtatct cttatagaaa tccaagccta tcatgtaaaa tgtagctagt      540

attaaaaaga acagattatc tgtcttttat cgcacattaa gcctctatag ttactaggaa      600

atattatatg caaattaacc ggggcagggg agtagccgag cttctcccac aagtctgtgc      660

gagggggccg gcgcgggcct agagatggcg gcgtcggatc gagggcctat ttcccatgat      720

tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac      780

tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta      840

gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa      900

gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg tgcccctcct      960

ttccgtttca gagctaggcc agcatgagga tcacccatgc ctgcagggcc tagcaagttg     1020

aaataaggct agtccgttat caacttggcc agcatgagga tcacccatgc ctgcagggcc     1080

aagtggcacc gagtcggtgc aacaaagcgc aagtggttta gtggtaaaat ccaacgttgc     1140

catcgttggg cccccggttc gattccgggc ttgcgcacaa agcggcagga ggtttcagag     1200

ctaggccagc atgaggatca cccatgcctg cagggcctag caagttgaaa taaggctagt     1260

ccgttatcaa cttggccagc atgaggatca cccatgcctg cagggccaag tggcaccgag     1320

tcggtgcttt tttt                                                       1334


<210>  7
<211>  178
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding a modified tracrRNA

<400>  7
ggaaccattc aaaacagcat agcaagttaa aataaggcta gtccgttatc aacttgaaaa       60

agtggcaccg agtcggtgcg ggagcggcca gcatgaggat cacccatgcc tgcagggccg      120

ccacgagcgg ggccaacatg aggatcaccc atgtctgcag ggccccgctc gtgttccc        178


<210>  8
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding crUT2

<400>  8
ttgaataaag ggcagtttta gagctatgct gttttgtttt ttt                         43


<210>  9
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding crUT16

<400>  9
gagagcagca gttggtttta gagctatgct gttttgtttt ttt                         43


<210>  10
<211>  157
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding dgFST

<400>  10
caaagcggca ggaggtttca gagctaggcc agcatgagga tcacccatgc ctgcagggcc       60

tagcaagttg aaataaggct agtccgttat caacttggcc agcatgagga tcacccatgc      120

ctgcagggcc aagtggcacc gagtcggtgc ttttttt                               157


<210>  11
<211>  157
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding dgEef1a2

<400>  11
tgcccctcct ttccgtttca gagctaggcc agcatgagga tcacccatgc ctgcagggcc       60

tagcaagttg aaataaggct agtccgttat caacttggcc agcatgagga tcacccatgc      120

ctgcagggcc aagtggcacc gagtcggtgc ttttttt                               157


<210>  12
<211>  157
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding dgUtrnNT2

<400>  12
ccagcacgca cgacgtttca gagctaggcc agcatgagga tcacccatgc ctgcagggcc       60

tagcaagttg aaataaggct agtccgttat caacttggcc agcatgagga tcacccatgc      120

ctgcagggcc aagtggcacc gagtcggtgc ttttttt                               157


<210>  13
<211>  157
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding dgUtrn

<400>  13
ttgaataaag ggcagtttca gagctaggcc agcatgagga tcacccatgc ctgcagggcc       60

tagcaagttg aaataaggct agtccgttat caacttggcc agcatgagga tcacccatgc      120

ctgcagggcc aagtggcacc gagtcggtgc ttttttt                               157


<210>  14
<211>  157
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding dgUtrnT2

<400>  14
ttgaataaag ggcagtttca gagctaggcc agcatgagga tcacccatgc ctgcagggcc       60

tagcaagttg aaataaggct agtccgttat caacttggcc agcatgagga tcacccatgc      120

ctgcagggcc aagtggcacc gagtcggtgc ttttttt                               157


<210>  15
<211>  157
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding dgUtrnT16

<400>  15
gagagcagca gttggtttca gagctaggcc agcatgagga tcacccatgc ctgcagggcc       60

tagcaagttg aaataaggct agtccgttat caacttggcc agcatgagga tcacccatgc      120

ctgcagggcc aagtggcacc gagtcggtgc ttttttt                               157


<210>  16
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding a native MS2-binding loop

<400>  16
ggccaacatg aggatcaccc atgtctgcag ggcc                                   34


<210>  17
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding a modified MS2-binding loop

<400>  17
tgctgaacat gaggatcacc catgtctgca gcagca                                 36


<210>  18
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding a modified MS2-binding loop

<400>  18
gggccaacat gaggatcacc catgtctgca gggccc                                 36


<210>  19
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding a modified MS2-binding loop

<400>  19
ggccagcatg aggatcaccc atgcctgcag ggcc                                   34


<210>  20
<211>  83
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  20
aacaaagcgc aagtggttta gtggtaaaat ccaacgttgc catcgttggg cccccggttc       60

gattccgggc ttgcgcacga aat                                               83


<210>  21
<211>  77
<212>  DNA
<213>  Zea mays

<400>  21
aacaaagcac cagtggtcta gtggtagaat agtaccctgc cacggtacag acccgggttc       60

gattcccggc tggtgca                                                      77


<210>  22
<211>  111
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding a hammerhead RNA

<400>  22
ggccggcatg gtcccagcct cctcgctggc gccggctggg caacatgctt cggcatggcg       60

aatgggactg ctggctgatg agtccgtgag gacgaaacga gtaagctcgt c               111


<210>  23
<211>  901
<212>  DNA
<213>  Homo sapiens

<400>  23
ggcccggtct ttggcttggc atcctgaccc catatgagca tcagctacaa ggcgctgagg       60

tgcagcgggg tggggcgctg ggcggggggg cctgggtctg tctggatctg actcgccctt      120

ggctggcgct gtttcccagc agcagccgga ggtcggcgca cccggagggg agggtccctg      180

gaagatgtca gtgggtctgg gagcgggctt ccggcgttcc ctgcaccgtg ggagaccagc      240

ctctcagggg gagggtggtt ctgcgctgga tcctcggggc ctgtcatggt gcgcccagga      300

gggcaggcac gtgaggacag ggactggaaa ccagcagatt tccaccctga ggcctgcacc      360

cccgggcctc attagggaga gcccctcaga gccgggcttc gttggttctg gggcgtcccc      420

catgagcagg gccggggagg ggccggtaga cccaggctcg tctcccaggc tgcagcccac      480

ctgctcccct cccccgcctg ccggctccgg tcctcggcgt ctgccctgtc cccggggacc      540

gcttttcgcg gctcaagcgt gttcctgccc tgagccggct ctcgccccgt ctcccgggcc      600

cgccgcgctc tccccgcgcc gtctccgtcc cggtccctcc ctcccgccgc ctccctgccc      660

tgccccccgc cccgcccccg cccgcggcgc gtttctcccc cgcctcccgc gtccgtcttt      720

gcagcccgcg cctcccgcat cgcctcgcgt ccccgtggcg cccgcccgcg cgcgtccgcg      780

ccccgccccc tcccgcgcgg ttccgcattg gcgtgctgca gggcgcggtg cactgcgccg      840

ccaccgtcaa taggtggacc ccctcccgga gataaaaccg ccggcgccgg cgccgccagt      900

c                                                                      901


<210>  24
<211>  721
<212>  DNA
<213>  Homo sapiens

<400>  24
ggagccgagg agactgagag acagacagag gcacacagga cagaaactgg ggagtctcca       60

ggcgggagag gaaggggggg ccagaccgcc tacgtcggcg cccccgctcc gggctccgac      120

tccagacgcc gcgaagtgaa aggggagaaa agaaagggag agggcgaggc tgtgccgcgg      180

ggagaccggg cctgaggtgt taaacatttt tgtttgcttc cgactagtcc agacgaaggg      240

ccgcgtctcg gtagcgctct gccagggtgg aaggtgccgg ggccggggtt cctagcaaca      300

cctctgggct gggggtggct gcaaagtcag gcactcacag acccagacac aaaacctcgc      360

gggtcccgcg cccaggctgc gggtgcccgg aaccgccgcg aggccggcgc gctccgaccc      420

gacccggggc gggatatttg ggcagcccgg ggctcttcgg ccgtttgcaa aagtctcttt      480

ggagcggagg agaggcagca cggagacaaa ctcccgggtt ccccccgcca ccgcctccag      540

cgcccccacc gcgccctccc tctcacactc gcgcgcgcgc gcacacacac tcacacacac      600

actcacacac acacccgcca ccccgggcgc gccggcgctg ccggcgagcg gcggcgagca      660

ggacttgaag tgggtgttct tccccactcc ccacccccga cgcgtagccc ccaacccccg      720

c                                                                      721


<210>  25
<211>  633
<212>  DNA
<213>  Homo sapiens

<400>  25
ttaaaaaaaa gaatttaaaa aagtctctgt gaatgcttca gaagttaccg tttacacccc       60

agaagtactt gcagcacatc cacaagtaaa aacacacaac gaatgccaga gtttcgtgtg      120

ttttttaacc gacatctttg tggctgtgaa caaacttcat aaataaaata gaatcaaatg      180

cttctgacct agagagctgg gtctgcaaac ttttttttta tcgtattccg caacagttaa      240

ataaaaaatt aaaaactcaa catgtctcct tgtaaactac atcaattaac aaacacacta      300

tgtccattat caaatataat agaaaaaata taggaaaata gaaaatagaa aaatatagga      360

aaatagaaac ttttaagcca cggtgaaaat gtttctataa atgagtggtt ctaatgtttt      420

cgtgagcgcc cattttgggg agcaccgcca gctgcccgtt caggagtgtg cagcaaactc      480

agctgagaga gaaaattgga acaaaagcag gtgctcgcgg gtacctgggc ctagcctctt      540

agtgcggcca gccaggccaa tcacggcccc cggctgaacc acgtggggcc ccgcggagcc      600

tatggtgcgg cggccggccc gccggtccgc gct                                   633


<210>  26
<211>  721
<212>  DNA
<213>  Homo sapiens

<400>  26
gtggctctgc aacttctgtc aaaagggctc tttggcaaca ggaaaaacgt catggctcca       60

ttgtattgta gaggatggga atgggtgttc cggctaaatt ctccctcccc tttccctcca      120

cagctcagat ggcaaatgtg cgacccaggg acctcccgct ccagcagacc tgtgcgcaca      180

actttgcaca gattacctgc taagtcagag ccgaaaggta acacagatgc caaaggataa      240

taaaggtgaa tgagatttac tcaaaattgg aaacttggtg tttggttttt caggagaaca      300

atcaacgact gtgatttgaa gttcaccagg gtattctgag agatctaatc aaagatagag      360

tgctggtttg aaattattaa aaggtaacag taaaagggag agcaaaaccc cagtcccaac      420

gcaacccata aatctacttt gtcttcctcg aaagaggggc gcgggtgggc gcgtctcccc      480

gcgagcatct cacctaaggg ggaatccctt tcagcgcacg gcgaagttcc ccctcggctg      540

tcccacctgg cagtccctct aggatttcgg ccagtcccta attggctcca gcaatgtcca      600

gccggagctt ctttgggcct ccgagtggga gaaaagtgag agcaggtgct tccccagcgg      660

cgcgctccgc tagggcccgg caggatcccg cccccaagtc ggggaaagtt ggtcggcgcc      720

t                                                                      721


<210>  27
<211>  361
<212>  DNA
<213>  Homo sapiens

<400>  27
aactaggggt aaaaaaaaaa tcagcaacgt cagcaaactg agatggggtg agttggaagg       60

cagattggaa tttatctctt aaaaaaatat caccctaact agagacctgt tttgcctaag      120

gggacgtgac tcacattttc ggataatctg aataagggga attgtgtctg ctcgaggcat      180

ccattctggt tcggtctccg gactcccggc tcccggcacg cacggttcac tctggagcgc      240

gcgccccagg ccagccaagc gccgagccgg gctgctgcgg gctgggaggg cgcgcagggc      300

cggcgctgat tgacggggcg cgcagtcagg tgacttgggg cgccaagttc ccgacgcggt      360

g                                                                      361


<210>  28
<211>  721
<212>  DNA
<213>  Homo sapiens

<400>  28
taagaagctt tcagcaagtg cagactactc ttacccactt cccccaagca cagttggggt       60

gggggacagc tgaagaggtg gaaacatgtg cctgagaatc ctaatgaaat cggggtaaag      120

gagcctggaa cacatcctgt gaccccgcct gtactgtagg aagccagtct ctggaaagta      180

aaatggaagg gctgcttggg aactttgagg atatttagcc caccccctca tttttacttg      240

gggaaactaa ggcccagaga cctaaggtga ctgcctaagt tagcaaggag aagtcttggg      300

tattcatccc aggttggggg gacccaatta tttctcaatc ccattgtatt ctggaatggg      360

caatttgtcc acgtcactgt gacctaggaa cacgcgaatg agaacccaca gctgagggcc      420

tctgcgcaca gaacagctgt tctccccagg aaatcaactt tttttaattg agaagctaaa      480

aaattattct aagagaggta gcccatccta aaaatagctg taatgcagaa gttcatgttc      540

aaccaatcat ttttgcttac gatgcaaaaa ttgaaaacta agtttattag agaggttaga      600

gaaggaggag ctctaagcag aaaaaatcct gtgccgggaa accttgattg tggcttttta      660

atgaatgaag aggcctccct gagcttacaa tataaaaggg ggacagagag gtgaaggtct      720

a                                                                      721


<210>  29
<211>  861
<212>  DNA
<213>  Homo sapiens

<400>  29
gtcgcccctc tcccccgccc cggtgggcag actgcgggtc tgcgccgtcc ggggttctgc       60

gtcgcagctg ccggccggag tcagcttcca tagaggccac acggaactgc ctggcgctcc      120

tcgggctgtg ggacccgtgg ggttaagtct gagtccccgc ccggcgagga gcagagagcg      180

cagagttggg gcggtacagg ccgccaggca gccggcgggg ctaggagagg gaggaaaggc      240

gggatcctcc gggaagtcga ttctccggcg tccgcctgcg gccactgcca aatcttcccc      300

atttctttcg tctactccct ccccttttcc ctcgaggacc gctgagtcca gagtttctag      360

gatgggggtg gggcgctgtc agcagaaaaa gccaagtctt tgggcggcac ccgagcacgt      420

ccaaactctc ccatcccact ggcctgcgcc ggggtagaat gtgcccggtg aacagagagc      480

ctgggaggga cgcggtgacc tggggagaag gggaaccctg tagggtctgg gcgaggctgc      540

agagccctct cctagccaaa gctgcccaaa ctttcttccc ctggagtctc cttccacccc      600

tctccctccc cttcctcctg gacaccccct taaacggtct ccgccttccc ttctctcctc      660

ttctctcccc acctcgatcc accccttttc gtcttcgccc gctccccccg ctctcctgtc      720

ctcctcctcc ctccctcttt gggcatccgc cccgtcaatc tccgccgccg ccggccccaa      780

cccggcccct ctccgcctcc caggctctca gagcgcccca ggctccagta gagccgccct      840

cagttctgcg cggagcgggg c                                                861


<210>  30
<211>  4101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding Cas9

<400>  30
gacaagaagt acagcatcgg cctggacatc ggcaccaact ctgtgggctg ggccgtgatc       60

accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac      120

agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc      180

acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat      240

ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg      300

gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac      360

atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa      420

ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg      480

atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg      540

gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc      600

aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg      660

ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg      720

attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat      780

gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag      840

atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg      900

ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg      960

atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag     1020

cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc     1080

tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa     1140

aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag     1200

cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc     1260

attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag     1320

aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga     1380

ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg     1440

gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac     1500

ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat     1560

aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc     1620

ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg     1680

aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc     1740

ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc     1800

aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg     1860

accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac     1920

ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg     1980

ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat     2040

ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc     2100

ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac     2160

gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg     2220

aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc     2280

gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg     2340

aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg     2400

gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat     2460

atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc     2520

gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac     2580

aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac     2640

tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc     2700

aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg     2760

gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact     2820

aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag     2880

ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac     2940

caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac     3000

cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg     3060

atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac     3120

atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct     3180

ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc     3240

accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag     3300

acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc     3360

agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat     3420

tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa     3480

gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt     3540

ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac     3600

tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag     3660

aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac     3720

tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag     3780

cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc     3840

ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc     3900

atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct     3960

gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag     4020

gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac     4080

ctgtctcagc tgggaggcga c                                               4101


<210>  31
<211>  1368
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary Cas9 amino acid sequence

<400>  31

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Ile Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Leu Asn Ser Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Ala Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Ile Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Leu Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Arg Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Thr Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Phe Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


<210>  32
<211>  4101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding dCas9

<400>  32
gacaagaagt actccattgg gctcgctatc ggcacaaaca gcgtcggctg ggccgtcatt       60

acggacgagt acaaggtgcc gagcaaaaaa ttcaaagttc tgggcaatac cgatcgccac      120

agcataaaga agaacctcat tggcgccctc ctgttcgact ccggggagac ggccgaagcc      180

acgcggctca aaagaacagc acggcgcaga tatacccgca gaaagaatcg gatctgctac      240

ctgcaggaga tctttagtaa tgagatggct aaggtggatg actctttctt ccataggctg      300

gaggagtcct ttttggtgga ggaggataaa aagcacgagc gccacccaat ctttggcaat      360

atcgtggacg aggtggcgta ccatgaaaag tacccaacca tatatcatct gaggaagaag      420

cttgtagaca gtactgataa ggctgacttg cggttgatct atctcgcgct ggcgcatatg      480

atcaaatttc ggggacactt cctcatcgag ggggacctga acccagacaa cagcgatgtc      540

gacaaactct ttatccaact ggttcagact tacaatcagc ttttcgaaga gaacccgatc      600

aacgcatccg gagttgacgc caaagcaatc ctgagcgcta ggctgtccaa atcccggcgg      660

ctcgaaaacc tcatcgcaca gctccctggg gagaagaaga acggcctgtt tggtaatctt      720

atcgccctgt cactcgggct gacccccaac tttaaatcta acttcgacct ggccgaagat      780

gccaagcttc aactgagcaa agacacctac gatgatgatc tcgacaatct gctggcccag      840

atcggcgacc agtacgcaga cctttttttg gcggcaaaga acctgtcaga cgccattctg      900

ctgagtgata ttctgcgagt gaacacggag atcaccaaag ctccgctgag cgctagtatg      960

atcaagcgct atgatgagca ccaccaagac ttgactttgc tgaaggccct tgtcagacag     1020

caactgcctg agaagtacaa ggaaattttc ttcgatcagt ctaaaaatgg ctacgccgga     1080

tacattgacg gcggagcaag ccaggaggaa ttttacaaat ttattaagcc catcttggaa     1140

aaaatggacg gcaccgagga gctgctggta aagcttaaca gagaagatct gttgcgcaaa     1200

cagcgcactt tcgacaatgg aagcatcccc caccagattc acctgggcga actgcacgct     1260

atcctcaggc ggcaagagga tttctacccc tttttgaaag ataacaggga aaagattgag     1320

aaaatcctca catttcggat accctactat gtaggccccc tcgcccgggg aaattccaga     1380

ttcgcgtgga tgactcgcaa atcagaagag accatcactc cctggaactt cgaggaagtc     1440

gtggataagg gggcctctgc ccagtccttc atcgaaagga tgactaactt tgataaaaat     1500

ctgcctaacg aaaaggtgct tcctaaacac tctctgctgt acgagtactt cacagtttat     1560

aacgagctca ccaaggtcaa atacgtcaca gaagggatga gaaagccagc attcctgtct     1620

ggagagcaga agaaagctat cgtggacctc ctcttcaaga cgaaccggaa agttaccgtg     1680

aaacagctca aagaagacta tttcaaaaag attgaatgtt tcgactctgt tgaaatcagc     1740

ggagtggagg atcgcttcaa cgcatccctg ggaacgtatc acgatctcct gaaaatcatt     1800

aaagacaagg acttcctgga caatgaggag aacgaggaca ttcttgagga cattgtcctc     1860

acccttacgt tgtttgaaga tagggagatg attgaagaac gcttgaaaac ttacgctcat     1920

ctcttcgacg acaaagtcat gaaacagctc aagaggcgcc gatatacagg atgggggcgg     1980

ctgtcaagaa aactgatcaa tgggatccga gacaagcaga gtggaaagac aatcctggat     2040

tttcttaagt ccgatggatt tgccaaccgg aacttcatgc agttgatcca tgatgactct     2100

ctcaccttta aggaggacat ccagaaagca caagtttctg gccaggggga cagtcttcac     2160

gagcacatcg ctaatcttgc aggtagccca gctatcaaaa agggaatact gcagaccgtt     2220

aaggtcgtgg atgaactcgt caaagtaatg ggaaggcata agcccgagaa tatcgttatc     2280

gagatggccc gagagaacca aactacccag aagggacaga agaacagtag ggaaaggatg     2340

aagaggattg aagagggtat aaaagaactg gggtcccaaa tccttaagga acacccagtt     2400

gaaaacaccc agcttcagaa tgagaagctc tacctgtact acctgcagaa cggcagggac     2460

atgtacgtgg atcaggaact ggacatcaat cggctctccg actacgacgt ggctgctatc     2520

gtgccccagt cttttctcaa agatgattct attgataata aagtgttgac aagatccgat     2580

aaagctagag ggaagagtga taacgtcccc tcagaagaag ttgtcaagaa aatgaaaaat     2640

tattggcggc agctgctgaa cgccaaactg atcacacaac ggaagttcga taatctgact     2700

aaggctgaac gaggtggcct gtctgagttg gataaagccg gcttcatcaa aaggcagctt     2760

gttgagacac gccagatcac caagcacgtg gcccaaattc tcgattcacg catgaacacc     2820

aagtacgatg aaaatgacaa actgattcga gaggtgaaag ttattactct gaagtctaag     2880

ctggtctcag atttcagaaa ggactttcag ttttataagg tgagagagat caacaattac     2940

caccatgcgc atgatgccta cctgaatgca gtggtaggca ctgcacttat caaaaaatat     3000

cccaagcttg aatctgaatt tgtttacgga gactataaag tgtacgatgt taggaaaatg     3060

atcgcaaagt ctgagcagga aataggcaag gccaccgcta agtacttctt ttacagcaat     3120

attatgaatt ttttcaagac cgagattaca ctggccaatg gagagattcg gaagcgacca     3180

cttatcgaaa caaacggaga aacaggagaa atcgtgtggg acaagggtag ggatttcgcg     3240

acagtccgga aggtcctgtc catgccgcag gtgaacatcg ttaaaaagac cgaagtacag     3300

accggaggct tctccaagga aagtatcctc ccgaaaagga acagcgacaa gctgatcgca     3360

cgcaaaaaag attgggaccc caagaaatac ggcggattcg attctcctac agtcgcttac     3420

agtgtactgg ttgtggccaa agtggagaaa gggaagtcta aaaaactcaa aagcgtcaag     3480

gaactgctgg gcatcacaat catggagcga tcaagcttcg aaaaaaaccc catcgacttt     3540

ctcgaggcga aaggatataa agaggtcaaa aaagacctca tcattaagct tcccaagtac     3600

tctctctttg agcttgaaaa cggccggaaa cgaatgctcg ctagtgcggg cgagctgcag     3660

aaaggtaacg agctggcact gccctctaaa tacgttaatt tcttgtatct ggccagccac     3720

tatgaaaagc tcaaagggtc tcccgaagat aatgagcaga agcagctgtt cgtggaacaa     3780

cacaaacact accttgatga gatcatcgag caaataagcg aattctccaa aagagtgatc     3840

ctcgccgacg ctaacctcga taaggtgctt tctgcttaca ataagcacag ggataagccc     3900

atcagggagc aggcagaaaa cattatccac ttgtttactc tgaccaactt gggcgcgcct     3960

gcagccttca agtacttcga caccaccata gacagaaagc ggtacacctc tacaaaggag     4020

gtcctggacg ccacactgat tcatcagtca attacggggc tctatgaaac aagaatcgac     4080

ctctctcagc tcggtggaga c                                               4101


<210>  33
<211>  1367
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary dCas9 amino acid sequence

<400>  33

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly 
    1355                 1360                 1365         


<210>  34
<211>  1419
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding a MS2-transcriptional activator 
       fusion protein

<400>  34
gcttcaaact ttactcagtt cgtgctcgtg gacaatggtg ggacagggga tgtgacagtg       60

gctccttcta atttcgctaa tggggtggca gagtggatca gctccaactc acggagccag      120

gcctacaagg tgacatgcag cgtcaggcag tctagtgccc agaagagaaa gtataccatc      180

aaggtggagg tccccaaagt ggctacccag acagtgggcg gagtcgaact gcctgtcgcc      240

gcttggaggt cctacctgaa catggagctc actatcccaa ttttcgctac caattctgac      300

tgtgaactca tcgtgaaggc aatgcagggg ctcctcaaag acggtaatcc tatcccttcc      360

gccatcgccg ctaactcagg tatctacagc gctggaggag gtggaagcgg aggaggagga      420

agcggaggag gaggtagcgg acctaagaaa aagaggaagg tggcggccgc tggatcccct      480

tcagggcaga tcagcaacca ggccctggct ctggccccta gctccgctcc agtgctggcc      540

cagactatgg tgccctctag tgctatggtg cctctggccc agccacctgc tccagcccct      600

gtgctgaccc caggaccacc ccagtcactg agcgctccag tgcccaagtc tacacaggcc      660

ggcgagggga ctctgagtga agctctgctg cacctgcagt tcgacgctga tgaggacctg      720

ggagctctgc tggggaacag caccgatccc ggagtgttca cagatctggc ctccgtggac      780

aactctgagt ttcagcagct gctgaatcag ggcgtgtcca tgtctcatag tacagccgaa      840

ccaatgctga tggagtaccc cgaagccatt acccggctgg tgaccggcag ccagcggccc      900

cccgaccccg ctccaactcc cctgggaacc agcggcctgc ctaatgggct gtccggagat      960

gaagacttct caagcatcgc tgatatggac tttagtgccc tgctgtcaca gatttcctct     1020

agtgggcagg gaggaggtgg aagcggcttc agcgtggaca ccagtgccct gctggacctg     1080

ttcagcccct cggtgaccgt gcccgacatg agcctgcctg accttgacag cagcctggcc     1140

agtatccaag agctcctgtc tccccaggag ccccccaggc ctcccgaggc agagaacagc     1200

agcccggatt cagggaagca gctggtgcac tacacagcgc agccgctgtt cctgctggac     1260

cccggctccg tggacaccgg gagcaacgac ctgccggtgc tgtttgagct gggagagggc     1320

tcctacttct ccgaagggga cggcttcgcc gaggacccca ccatctccct gctgacaggc     1380

tcggagcctc ccaaagccaa ggaccccact gtctcctga                            1419


<210>  35
<211>  473
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary MS2-p65-HSF1 amino acid sequence

<400>  35

Met Ala Ser Asn Phe Thr Gln Phe Val Leu Val Asp Asn Gly Gly Thr 
1               5                   10                  15      


Gly Asp Val Thr Val Ala Pro Ser Asn Phe Ala Asn Gly Val Ala Glu 
            20                  25                  30          


Trp Ile Ser Ser Asn Ser Arg Ser Gln Ala Tyr Lys Val Thr Cys Ser 
        35                  40                  45              


Val Arg Gln Ser Ser Ala Gln Lys Arg Lys Tyr Thr Ile Lys Val Glu 
    50                  55                  60                  


Val Pro Lys Val Ala Thr Gln Thr Val Gly Gly Val Glu Leu Pro Val 
65                  70                  75                  80  


Ala Ala Trp Arg Ser Tyr Leu Asn Met Glu Leu Thr Ile Pro Ile Phe 
                85                  90                  95      


Ala Thr Asn Ser Asp Cys Glu Leu Ile Val Lys Ala Met Gln Gly Leu 
            100                 105                 110         


Leu Lys Asp Gly Asn Pro Ile Pro Ser Ala Ile Ala Ala Asn Ser Gly 
        115                 120                 125             


Ile Tyr Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Pro Lys Lys Lys Arg Lys Val Ala Ala Ala Gly Ser 
145                 150                 155                 160 


Pro Ser Gly Gln Ile Ser Asn Gln Ala Leu Ala Leu Ala Pro Ser Ser 
                165                 170                 175     


Ala Pro Val Leu Ala Gln Thr Met Val Pro Ser Ser Ala Met Val Pro 
            180                 185                 190         


Leu Ala Gln Pro Pro Ala Pro Ala Pro Val Leu Thr Pro Gly Pro Pro 
        195                 200                 205             


Gln Ser Leu Ser Ala Pro Val Pro Lys Ser Thr Gln Ala Gly Glu Gly 
    210                 215                 220                 


Thr Leu Ser Glu Ala Leu Leu His Leu Gln Phe Asp Ala Asp Glu Asp 
225                 230                 235                 240 


Leu Gly Ala Leu Leu Gly Asn Ser Thr Asp Pro Gly Val Phe Thr Asp 
                245                 250                 255     


Leu Ala Ser Val Asp Asn Ser Glu Phe Gln Gln Leu Leu Asn Gln Gly 
            260                 265                 270         


Val Ser Met Ser His Ser Thr Ala Glu Pro Met Leu Met Glu Tyr Pro 
        275                 280                 285             


Glu Ala Ile Thr Arg Leu Val Thr Gly Ser Gln Arg Pro Pro Asp Pro 
    290                 295                 300                 


Ala Pro Thr Pro Leu Gly Thr Ser Gly Leu Pro Asn Gly Leu Ser Gly 
305                 310                 315                 320 


Asp Glu Asp Phe Ser Ser Ile Ala Asp Met Asp Phe Ser Ala Leu Leu 
                325                 330                 335     


Ser Gln Ile Ser Ser Ser Gly Gln Gly Gly Gly Gly Ser Gly Phe Ser 
            340                 345                 350         


Val Asp Thr Ser Ala Leu Leu Asp Leu Phe Ser Pro Ser Val Thr Val 
        355                 360                 365             


Pro Asp Met Ser Leu Pro Asp Leu Asp Ser Ser Leu Ala Ser Ile Gln 
    370                 375                 380                 


Glu Leu Leu Ser Pro Gln Glu Pro Pro Arg Pro Pro Glu Ala Glu Asn 
385                 390                 395                 400 


Ser Ser Pro Asp Ser Gly Lys Gln Leu Val His Tyr Thr Ala Gln Pro 
                405                 410                 415     


Leu Phe Leu Leu Asp Pro Gly Ser Val Asp Thr Gly Ser Asn Asp Leu 
            420                 425                 430         


Pro Val Leu Phe Glu Leu Gly Glu Gly Ser Tyr Phe Ser Glu Gly Asp 
        435                 440                 445             


Gly Phe Ala Glu Asp Pro Thr Ile Ser Leu Leu Thr Gly Ser Glu Pro 
    450                 455                 460                 


Pro Lys Ala Lys Asp Pro Thr Val Ser 
465                 470             


<210>  36
<211>  276
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding a 7SK promoter

<400>  36
tttaattcta gtactatgca tcgtctcatt gtctgcagta tttagcatgc cccacccatc       60

tgcaaggcat tctggatagt gtcaaaacag ccggaaatca agtccgttta tctcaaactt      120

tagcattttg ggaataaatg atatttgcta tgctggttaa attagatttt agttaaattt      180

cctgctgaag ctctagtacg ataagcaact tgacctaagt gtaaagttga gacttccttc      240

aggtttatat agcttgtgcg ccgcttgggt acctcg                                276


<210>  37
<211>  361
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding a Spc5.12 promoter

<400>  37
caccgcggtg gcggccgtcc gccctcggca ccatcctcac gacacccaaa tatggcgacg       60

ggtgaggaat ggtggggagt tatttttaga gcggtgagga aggtgggcag gcagcaggtg      120

ttggcgctct aaaaataact cccgggagtt atttttagag cggaggaatg gtggacaccc      180

aaatatggcg acggttcctc acccgtcgcc atatttgggt gtccgccctc ggccggggcc      240

gcattcctgg gggccgggcg gtgctcccgc ccgcctcgat aaaaggctcc ggggccggcg      300

gcggcccacg agctacccgg aggagcggga ggcgccaagc tctagaacta gtggatcccc      360

c                                                                      361


<210>  38
<211>  410
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding a Col1a2 promoter

<400>  38
agatctgtaa agagcccacg taggtgtcct aaagtgcttc caaacttggc aagggcgaga       60

gagggcgggt ggctggggag ggcggaggta tgcagacagg gagtcagagt tccccctcga      120

aagcctcaaa agtgtccacg tcctcaaaaa gaatggaacc aatttaagaa gccccgtagc      180

cacgtccctc ccccctcggc tccctcccct gctcccccgc agtctcctcc cagcactgag      240

tcccgggccc ctagccctag ccctcccatt ggtggagacg tttttggagg caccctccgg      300

ctggggaaac ttttcccata taaataaggc aggtctgggc tttattattt tagcaccacg      360

gcagcaggag gtttcgacta agttggaggg aacggtccac gattgcatgc                 410


<210>  39
<211>  316
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding an mU6 promoter

<400>  39
gatccgacgc cgccatctct aggcccgcgc cggccccctc gcacagactt gtgggagaag       60

ctcggctact cccctgcccc ggttaatttg catataatat ttcctagtaa ctatagaggc      120

ttaatgtgcg ataaaagaca gataatctgt tctttttaat actagctaca ttttacatga      180

taggcttgga tttctataag agatacaaat actaaattat tattttaaaa aacagcacaa      240

aaggaaactc accctaactg taaagtaatt gtgtgttttg agactataaa tatcccttgg      300

agaaaagcct tgtttg                                                      316


<210>  40
<211>  250
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding an hU6 promoter

<400>  40
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

cgaaacaccg                                                             250


<210>  41
<211>  224
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding an H1 promoter

<400>  41
gaacgctgac gtcatcaacc cgctccaagg aatcgcgggc ccagtgtcac taggcgggaa       60

cacccagcgc gcgtgcgccc tggcaggaag atggctgtga gggacagggg agtggcgccc      120

tgcaatattt gcatgtcgct atgtgttctg ggaaatcacc ataaacgtga aatgtctttg      180

gatttgggaa tcttataagt tctgtatgag accactcttt ccca                       224


<210>  42
<211>  157
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding dgMyoD

<400>  42
agagttggta gagtgtttca gagctaggcc agcatgagga tcacccatgc ctgcagggcc       60

tagcaagttg aaataaggct agtccgttat caacttggcc agcatgagga tcacccatgc      120

ctgcagggcc aagtggcacc gagtcggtgc ttttttt                               157


<210>  43
<211>  157
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding dgMef2b

<400>  43
actgagcata gctcgtttca gagctaggcc agcatgagga tcacccatgc ctgcagggcc       60

tagcaagttg aaataaggct agtccgttat caacttggcc agcatgagga tcacccatgc      120

ctgcagggcc aagtggcacc gagtcggtgc ttttttt                               157


<210>  44
<211>  157
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding dgPax7

<400>  44
acaccggctg ccgtgtttca gagctaggcc agcatgagga tcacccatgc ctgcagggcc       60

tagcaagttg aaataaggct agtccgttat caacttggcc agcatgagga tcacccatgc      120

ctgcagggcc aagtggcacc gagtcggtgc ttttttt                               157


<210>  45
<211>  157
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding dgOCT4

<400>  45
ggggacctgc actggtttca gagctaggcc agcatgagga tcacccatgc ctgcagggcc       60

tagcaagttg aaataaggct agtccgttat caacttggcc agcatgagga tcacccatgc      120

ctgcagggcc aagtggcacc gagtcggtgc ttttttt                               157


<210>  46
<211>  157
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding dgSOX2

<400>  46
ccggcagcga ggctgtttca gagctaggcc agcatgagga tcacccatgc ctgcagggcc       60

tagcaagttg aaataaggct agtccgttat caacttggcc agcatgagga tcacccatgc      120

ctgcagggcc aagtggcacc gagtcggtgc ttttttt                               157


<210>  47
<211>  157
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding dgKLF

<400>  47
atagcaacga tggagtttca gagctaggcc agcatgagga tcacccatgc ctgcagggcc       60

tagcaagttg aaataaggct agtccgttat caacttggcc agcatgagga tcacccatgc      120

ctgcagggcc aagtggcacc gagtcggtgc ttttttt                               157


<210>  48
<211>  157
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding dgMYC

<400>  48
caaagcagag ggcggtttca gagctaggcc agcatgagga tcacccatgc ctgcagggcc       60

tagcaagttg aaataaggct agtccgttat caacttggcc agcatgagga tcacccatgc      120

ctgcagggcc aagtggcacc gagtcggtgc ttttttt                               157


<210>  49
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding crUCP1

<400>  49
gagtgacgcg cggcgtttta gagctatgct gttttgtttt ttt                         43


<210>  50
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding crPgc1a

<400>  50
gcgttacttc actggtttta gagctatgct gttttgtttt ttt                         43


<210>  51
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding crFST

<400>  51
caaagcggca ggaggtttta gagctatgct gttttgtttt ttt                         43


<210>  52
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding crUtrn

<400>  52
ttgaataaag ggcagtttta gagctatgct gttttgtttt ttt                         43


<210>  53
<211>  956
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding multiplex sgRNAs

<400>  53
aaaaaaagca ccagccggga atcgaacccg ggtctgtacc gtggcagggt actattctac       60

cactagacca ctggtgcttt gttgcaccga ctcggtgcca cttggccctg caggcatggg      120

tgatcctcat gctggccaag ttgataacgg actagcctta tttcaacttg ctaggccctg      180

caggcatggg tgatcctcat gctggcctag ctctgaaacg tcgtgcgtgc tggcaaacaa      240

ggcttttctc caagggatat ttatagtctc aaaacacaca attactttac agttagggtg      300

agtttccttt tgtgctgttt tttaaaataa taatttagta tttgtatctc ttatagaaat      360

ccaagcctat catgtaaaat gtagctagta ttaaaaagaa cagattatct gtcttttatc      420

gcacattaag cctctatagt tactaggaaa tattatatgc aaattaaccg gggcagggga      480

gtagccgagc ttctcccaca agtctgtgcg agggggccgg cgcgggccta gagatggcgg      540

cgtcggatcg agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct      600

gttagagaga taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg      660

tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg      720

gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg      780

tggaaaggac gaaacaccgt tgaataaagg gcagtttcag agctaggcca gcatgaggat      840

cacccatgcc tgcagggcct agcaagttga aataaggcta gtccgttatc aacttggcca      900

gcatgaggat cacccatgcc tgcagggcca agtggcaccg agtcggtgct tttttt          956


<210>  54
<211>  956
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding multiplex sgRNAs

<400>  54
aaaaaaagca ccagccggga atcgaacccg ggtctgtacc gtggcagggt actattctac       60

cactagacca ctggtgcttt gttgcaccga ctcggtgcca cttggccctg caggcatggg      120

tgatcctcat gctggccaag ttgataacgg actagcctta tttcaacttg ctaggccctg      180

caggcatggg tgatcctcat gctggcctag ctctgaaacg tcgtgcgtgc tggcaaacaa      240

ggcttttctc caagggatat ttatagtctc aaaacacaca attactttac agttagggtg      300

agtttccttt tgtgctgttt tttaaaataa taatttagta tttgtatctc ttatagaaat      360

ccaagcctat catgtaaaat gtagctagta ttaaaaagaa cagattatct gtcttttatc      420

gcacattaag cctctatagt tactaggaaa tattatatgc aaattaaccg gggcagggga      480

gtagccgagc ttctcccaca agtctgtgcg agggggccgg cgcgggccta gagatggcgg      540

cgtcggatcg agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct      600

gttagagaga taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg      660

tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg      720

gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg      780

tggaaaggac gaaacaccgt gcccctcctt tccgtttcag agctaggcca gcatgaggat      840

cacccatgcc tgcagggcct agcaagttga aataaggcta gtccgttatc aacttggcca      900

gcatgaggat cacccatgcc tgcagggcca agtggcaccg agtcggtgct tttttt          956


<210>  55
<211>  1107
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary DNA sequence encoding multiplex sgRNAs

<400>  55
aaaaaaagca ccgactcggt gccacttggc cctgcaggca tgggtgatcc tcatgctggc       60

caagttgata acggactagc cttatttcaa cttgctaggc cctgcaggca tgggtgatcc      120

tcatgctggc ctagctctga aactgccctt tattcaatgc accagccggg aatcgaaccc      180

gggtctgtac cgtggcaggg tactattcta ccactagacc actggtgctt tgttgcaccg      240

actcggtgcc acttggccct gcaggcatgg gtgatcctca tgctggccaa gttgataacg      300

gactagcctt atttcaactt gctaggccct gcaggcatgg gtgatcctca tgctggccta      360

gctctgaaac gtcgtgcgtg ctggcaaaca aggcttttct ccaagggata tttatagtct      420

caaaacacac aattacttta cagttagggt gagtttcctt ttgtgctgtt ttttaaaata      480

ataatttagt atttgtatct cttatagaaa tccaagccta tcatgtaaaa tgtagctagt      540

attaaaaaga acagattatc tgtcttttat cgcacattaa gcctctatag ttactaggaa      600

atattatatg caaattaacc ggggcagggg agtagccgag cttctcccac aagtctgtgc      660

gagggggccg gcgcgggcct agagatggcg gcgtcggatc gagggcctat ttcccatgat      720

tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac      780

tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta      840

gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa      900

gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg tgcccctcct      960

ttccgtttca gagctaggcc agcatgagga tcacccatgc ctgcagggcc tagcaagttg     1020

aaataaggct agtccgttat caacttggcc agcatgagga tcacccatgc ctgcagggcc     1080

aagtggcacc gagtcggtgc ttttttt                                         1107


<210>  56
<211>  174
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequencing product

<400>  56
acctagtgtg cctagagggg tgtgacacac attttcggac aatttgaata aagggcacgg       60

tgcgtgcgcg cggtgactat tccagcttct ggcttccagc acgcacgact ggttccggga      120

ttctcgcacc gcgcaccgca cggagccggc tgctgcgggc tgggagggcg ccta            174


<210>  57
<211>  671
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequencing product

<400>  57
gttttgagac tataaatatc ccttggagaa aagccttgtt tgttgaataa agggcagttt       60

cagagctagg ccagcatgag gatcacccat gcctgcaggg cctagcaagt tgaaataagg      120

ctagtccgtt atcaacttgg ccagcatgag gatcacccat gcctgcaggg ccaagtggca      180

ccgagtcggt gctttttttg agggcctatt tcccatgatt ccttcatatt tgcatatacg      240

atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa agatattagt      300

acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg      360

ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt      420

atatatcttg tggaaaggac gaaacaccgc aaagcggcag gaggtttcag agctaggcca      480

gcatgaggat cacccatgcc tgcagggcct agcaagttga aataaggcta gtccgttatc      540

aacttggcca gcatgaggat cacccatgcc tgcagggcca agtggcaccg agtcggtgct      600

ttttttgttt tagagctagc gaattcggct ccggtgcccg tcagtgggca gagcgcacat      660

cgcccacagt c                                                           671


<210>  58
<211>  219
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequencing product

<400>  58
gagactataa atatcccttg gagaaaagcc ttgtttgttg aataaagggc agtttcagag       60

ctaggccagc atgaggatca cccatgcctg cagggcctag caagttgaaa taaggctagt      120

ccgttatcaa cttgggccaa catgaggatc acccatgtct gcagggccca agtggcaccg      180

agtcggtgct ttttttgttt tagagctagc gaattcggc                             219


<210>  59
<211>  469
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequencing product

<400>  59
atcaacccgc tccaaggaat cgcgggccca gtgtcactag gcgggaacac ccagcgcgcg       60

tgcgccctgg caggaagatg gctgtgaggg acaggggagt ggcgccctgc aatatttgca      120

tgtcgctatg tgttctggga aatcaccata aacgtgaaat gtctttggat ttgggaatct      180

tataagttct gtatgagacc actctttccc aagagttggt agagtgtttc agagctaggc      240

cagcatgagg atcacccatg cctgcagggc ctagcaagtt gaaataaggc tagtccgtta      300

tcaacttggc cagcatgagg atcacccatg cctgcagggc caagtggcac cgagtcggtg      360

ctttttttct agcgcggccg cagtatgata cacttgatga agccgaattc tgcagatatc      420

catcacactg gcggccgctc gagcatgcat ctagagggcc caattcgcc                  469


<210>  60
<211>  423
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequencing product

<400>  60
gtggaaagga cgaaacaccg ttgaataaag ggcagtttca gagctaggcc agcatgagga       60

tcacccatgc ctgcagggcc tagcaagttg aaataaggct agtccgttat caacttggcc      120

agcatgagga tcacccatgc ctgcagggcc aagtggcacc gagtcggtgc aacaaagcac      180

cagtggtcta gtggtagaat agtaccctgc cacggtacag acccgggttc gattcccggc      240

tggtgcacaa agcggcagga ggtttcagag ctagggccaa catgaggatc acccatgtct      300

gcagggccct agcaagttga aataaggcta gtccgttatc aacttgggcc aacatgagga      360

tcacccatgt ctgcagggcc caagtggcac cgagtcggtg ctttttttaa gcttggcttg      420

aat                                                                    423


<210>  61
<211>  244
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequencing product

<400>  61
atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga       60

cgaaacaccg ttgaataaag ggcagtttca gagctaggcc agcatgagga tcacccatgc      120

ctgcagggcc tagcaagttg aaataaggct agtccgttat caacttgggc caacatgagg      180

atcacccatg tctgcagggc ccaagtggca ccgagtcggt gcttttttta agcttggctt      240

gaat                                                                   244


