                         SEQUENCE LISTING

<110>  Monsanto Technology LLC
 
<120>  PLANT REGULATORY ELEMENTS AND USES THEREOF

<130>  MONS:492WO

<150>  US 63/182,288
<151>  2021-04-30

<150>  US 63/295,061
<151>  2021-12-30

<160>  33    

<170>  PatentIn version 3.5

<210>  1
<211>  500
<212>  DNA
<213>  Artificial

<220>
<223>  DNA sequence of the synthetic snRNA promoter, P-GSP2262.

<400>  1
gttattgtta tatagcacgt tggaggcaga atcttcaacg tcttgagtgc aggtcttctg       60

aataaatctt ttttctcgac caagccgttg tgcaaaggtc aaaaaaggtg tctagcggct      120

gtttaaagaa aaggaatcaa aggaggaacg attgctgctg cttgctttgc tttctatcgc      180

caggattgac agttcgctga actgttggcc aagattacag aatctgtgaa gagtttattc      240

acgattaatc taggaggaag cgcgctttgt tggctgtagg cccaaagcag caatcaacag      300

agtgtgtgag ggccagctgc tggaaggttc ggttttttca acaggcccac acgcgcgggc      360

tgggctgggc tgggctggcc acgttccttc ggtagcagtt cggtggagca acgaggacgg      420

tggaatcgaa gtttagtacc acctcggctg aagatacgga gaaagaccag tttataacct      480

gcctctggag cctgctgctc                                                  500


<210>  2
<211>  500
<212>  DNA
<213>  Artificial

<220>
<223>  DNA sequence of the synthetic snRNA promoter, P-GSP2268.

<400>  2
ctcgaggagg tcgcagcggc gatttgttgc caacgtaacc ttcagctcgc gaggcaaaaa       60

ttctttttct tcgccgctca acacggaatc gaagaccacc tcgctgtttc tcgattttac      120

cttgtttcac tcagagtgag ctgagtgaga aaaccaataa taacggtgaa tcagcagcaa      180

gttcaaattg tattgcgttt tcttataagg aaacagcact acttactttt tagtaccctc      240

gttcaagaaa gaaggcccaa acgcaagact gggtcgctga gcaacagagg acgcggtggg      300

cgtgtgtgca aggcctctgc tgaagaagac aggaaggttg ggctactact gctaaaagat      360

aaagcccacg gcccagaagc ccaagatcgg ttcggtgctg ggagcgcgcg cgcttagcga      420

gcagccagta ggtttagtac cacatcggct gctcacaaag cgaaagacca gtttataagg      480

tgacctgctc actcactctc                                                  500


<210>  3
<211>  500
<212>  DNA
<213>  Artificial

<220>
<223>  DNA sequence of the synthetic snRNA promoter, P-GSP2269.

<400>  3
ttgtggaatc gaggagctgc tcaaagttca acgtaacctt tcagaagctg aattacaaaa       60

gaccacctcg acttctcacg gagtaccagc tgtgagctta ctctttagac tttagtggtg      120

tccaatcgac ttttttaaag aagatctggg tgtgcagttg ttctagacca gccgtacact      180

gagcaagagt ttttctttta gtaacagagt gcaaggttag tacagtcaac ggcaacagtt      240

taagtgagct taaagtcaag cgctggtgag gcccaacaag cgtcaacagc aacgagaggc      300

tctctgctgg gccagcaaaa actggaaagc gggccgaaga aacgcggctg gcccacctct      360

tcgctggctt tttattgaag cccagctctg ggtgggagcg caagcgctta cgagcgttgg      420

ccacgagcag agtttagtac cacatcggct gcgtagagcc agctagacca ctttataacg      480

caagctgcag cttgatcctc                                                  500


<210>  4
<211>  500
<212>  DNA
<213>  Artificial

<220>
<223>  DNA sequence of the synthetic snRNA promoter, P-GSP2272.

<400>  4
tatagacgat atcacgctgc tgccgtcaac agaaggtcgg tgaagctgaa aatttcgaac       60

ggtgtgtgct agtagttttc ttttttagca agaccacctc ttgttagctt cttttcaaaa      120

aggaactgag ctgacttgat actagtacgg aataacggaa taagctaatt tcagacggtg      180

tctgcaaacg tttttttagt accagcagtg tcgctgaagt gaagtgtgtg tccttcttct      240

acttgcaccc agctgaggcc caaagtgttc gcgtacacaa ggcgagaggc aaacgaggct      300

gggtgggccc acaaagaaag ccacttgctg cttgtcaagc taaagcccgt gctggctggc      360

tggctggccc acaagatata cagaagcgct ctggagcgcg gcgcttctgg aacgagcgct      420

aggcaatcga agtttagtac cacatcggct gctcaagcta gcgaacacca gcttataagc      480

ttaactccga gcctctgctc                                                  500


<210>  5
<211>  500
<212>  DNA
<213>  Artificial

<220>
<223>  DNA sequence of the synthetic snRNA promoter, P-GSP2273.

<400>  5
tctatttcaa gctgaactgc tgccctgctc ggtgttcagc agaagctcgg ccgtaaaagg       60

gaatcttggt tgtgctaaaa gtacttctgt actagttact cgcagctgac gcgcgcactg      120

aacgtacttg tgtttagtaa tttcgaaaaa ttcagatcaa aagccgtatt ctacgtaacg      180

attggggcag actgcagtac cagcactgac gacagcactt accagctctg tgaaacttca      240

cgaaacgcaa tcggaaaacg aggtgggctg tgttacacag ctgggcctgt gtcaacagca      300

acgaagccca cccaacagag gattgggcct cgatcaagcg gcctcacaac gtgcggacac      360

gcggtgcgag gccactctgt cagcagaagc ccggctggga gcaacgagca acttgtgcgc      420

tgcgctgaag gtttagtacc acatcggttg cgtaacgacc agcttaccac cttataagct      480

taaaccggag cgctgctttc                                                  500


<210>  6
<211>  280
<212>  DNA
<213>  Artificial

<220>
<223>  DNA sequence of the truncation variant synthetic snRNA promoter 
       P-GSP2262_TR, derived from P-GSP2262.

<400>  6
aatctgtgaa gagtttattc acgattaatc taggaggaag cgcgctttgt tggctgtagg       60

cccaaagcag caatcaacag agtgtgtgag ggccagctgc tggaaggttc ggttttttca      120

acaggcccac acgcgcgggc tgggctgggc tgggctggcc acgttccttc ggtagcagtt      180

cggtggagca acgaggacgg tggaatcgaa gtttagtacc acctcggctg aagatacgga      240

gaaagaccag tttataacct gcctctggag cctgctgctc                            280


<210>  7
<211>  300
<212>  DNA
<213>  Artificial

<220>
<223>  DNA sequence of the truncation variant synthetic snRNA promoter 
       P-GSP2268_TR, derived from P-GSP2268.

<400>  7
tcttataagg aaacagcact acttactttt tagtaccctc gttcaagaaa gaaggcccaa       60

acgcaagact gggtcgctga gcaacagagg acgcggtggg cgtgtgtgca aggcctctgc      120

tgaagaagac aggaaggttg ggctactact gctaaaagat aaagcccacg gcccagaagc      180

ccaagatcgg ttcggtgctg ggagcgcgcg cgcttagcga gcagccagta ggtttagtac      240

cacatcggct gctcacaaag cgaaagacca gtttataagg tgacctgctc actcactctc      300


<210>  8
<211>  300
<212>  DNA
<213>  Artificial

<220>
<223>  DNA sequence of the truncation variant synthetic snRNA promoter 
       P-GSP2269_TR, derived from P-GSP2269.

<400>  8
gtaacagagt gcaaggttag tacagtcaac ggcaacagtt taagtgagct taaagtcaag       60

cgctggtgag gcccaacaag cgtcaacagc aacgagaggc tctctgctgg gccagcaaaa      120

actggaaagc gggccgaaga aacgcggctg gcccacctct tcgctggctt tttattgaag      180

cccagctctg ggtgggagcg caagcgctta cgagcgttgg ccacgagcag agtttagtac      240

cacatcggct gcgtagagcc agctagacca ctttataacg caagctgcag cttgatcctc      300


<210>  9
<211>  288
<212>  DNA
<213>  Artificial

<220>
<223>  DNA sequence of the truncation variant synthetic snRNA promoter 
       P-GSP2272_TR, derived from P-GSP2272.

<400>  9
gctgaagtga agtgtgtgtc cttcttctac ttgcacccag ctgaggccca aagtgttcgc       60

gtacacaagg cgagaggcaa acgaggctgg gtgggcccac aaagaaagcc acttgctgct      120

tgtcaagcta aagcccgtgc tggctggctg gctggcccac aagatataca gaagcgctct      180

ggagcgcggc gcttctggaa cgagcgctag gcaatcgaag tttagtacca catcggctgc      240

tcaagctagc gaacaccagc ttataagctt aactccgagc ctctgctc                   288


<210>  10
<211>  282
<212>  DNA
<213>  Artificial

<220>
<223>  DNA sequence of the truncation variant synthetic snRNA promoter 
       P-GSP2273_TR, derived from P-GSP2273.

<400>  10
ttaccagctc tgtgaaactt cacgaaacgc aatcggaaaa cgaggtgggc tgtgttacac       60

agctgggcct gtgtcaacag caacgaagcc cacccaacag aggattgggc ctcgatcaag      120

cggcctcaca acgtgcggac acgcggtgcg aggccactct gtcagcagaa gcccggctgg      180

gagcaacgag caacttgtgc gctgcgctga aggtttagta ccacatcggt tgcgtaacga      240

ccagcttacc accttataag cttaaaccgg agcgctgctt tc                         282


<210>  11
<211>  2008
<212>  DNA
<213>  Zea mays


<220>
<221>  misc_feature
<222>  (1)..(2008)
<223>  DNA sequence of an EXP, EXP-Zm.UbqM1:1:9 comprised of a promoter,
       leader, and intron derived from a Zea may Mexicana Ubiquitin 
       gene.

<400>  11
gtcgtgcccc tctctagaga taaagagcat tgcatgtcta aagtataaaa aattaccaca       60

tatttttttg tcacacttat ttgaagtgta gtttatctat ctctatacat atatttaaac      120

ttcactctac aaataatata gtctataata ctaaaataat attagtgttt tagaggatca      180

tataaataaa ctgctagaca tggtctaaag gataattgaa tattttgaca atctacagtt      240

ttatcttttt agtgtgcatg tgatctctct gttttttttg caaatagctt gacctatata      300

atacttcatc cattttatta gtacatccat ttaggattta gggttgatgg tttctataga      360

ctaattttta gtacatccat tttattcttt ttagtctcta aattttttaa aactaaaact      420

ctattttagt tttttattta ataatttaga tataaaatga aataaaataa attgactaca      480

aataaaacaa atacccttta agaaataaaa aaactaagca aacatttttc ttgtttcgag      540

tagataatga caggctgttc aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc      600

agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtag ctgcctctgg      660

acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca tccagaaatt      720

gcgtggcgga gcggcagacg tgaggcggca cggcaggcgg cctcttcctc ctctcacggc      780

accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc ctcgcccgcc      840

gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttcg ttcggagcgc      900

acacacacgc aaccagatct cccccaaatc cagccgtcgg cacctccgct tcaaggtacg      960

ccgctcatcc tccccccccc cctctctcta ccttctctag atcggcgatc cggtccatgg     1020

ttagggcccg gtagttctac ttctgttcat gtttgtgtta gagcaaacat gttcatgttc     1080

atgtttgtga tgatgtggtc tggttgggcg gtcgttctag atcggagtag gatactgttt     1140

caagctacct ggtggattta ttaattttgt atctgtatgt gtgtgccata catcttcata     1200

gttacgagtt taagatgatg gatggaaata tcgatctagg ataggtatac atgttgatgc     1260

gggttttact gatgcatata cagagatgct ttttttctcg cttggttgtg atgatatggt     1320

ctggttgggc ggtcgttcta gatcggagta gaatactgtt tcaaactacc tggtggattt     1380

attaaaggat aaagggtcgt tctagatcgg agtagaatac tgtttcaaac tacctggtgg     1440

atttattaaa ggatctgtat gtatgtgcct acatcttcat agttacgagt ttaagatgat     1500

ggatggaaat atcgatctag gataggtata catgttgatg cgggttttac tgatgcatat     1560

acagagatgc tttttttcgc ttggttgtga tgatgtggtc tggttgggcg gtcgttctag     1620

atcggagtag aatactgttt caaactacct ggtggattta ttaattttgt atctttatgt     1680

gtgtgccata catcttcata gttacgagtt taagatgatg gatggaaata ttgatctagg     1740

ataggtatac atgttgatgt gggttttact gatgcatata catgatggca tatgcggcat     1800

ctattcatat gctctaacct tgagtaccta tctattataa taaacaagta tgttttataa     1860

ttattttgat cttgatatac ttggatgatg gcatatgcag cagctatatg tggatttttt     1920

agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcc gatgctcacc     1980

ctgttgtttg gtgatacttc tgcaggtc                                        2008


<210>  12
<211>  3750
<212>  DNA
<213>  Artificial

<220>
<223>  DNA sequence encoding a nuclear targeted Cas12a protein, 
       Cas12a_NLS.

<400>  12
atggcgggat ctaagaagag aagaattaaa caagattcga agctcgagaa gttcaccaac       60

tgctactcgc tgagcaagac gctgcggttc aaggcgatcc ccgtcgggaa gacccaggag      120

aacatcgaca acaagcggct cctggtcgag gacgagaagc gcgccgagga ctacaagggc      180

gtcaagaagc tgctggaccg gtactacctc tccttcatca acgacgtcct gcactcgatc      240

aagctcaaga acctgaacaa ctacatctcg ctgttccgca agaagacacg gaccgagaag      300

gagaacaagg agctcgagaa cctcgagatc aacctgcgca aggagatcgc gaaggcgttc      360

aagggcaacg aggggtacaa gagcctgttc aagaaagaca tcatcgagac catcctgccg      420

gagttcctgg acgacaagga cgagatcgcg ctggtgaact cgttcaacgg gttcaccacg      480

gccttcaccg ggtttttcga caaccgggag aacatgttca gcgaggaggc caagtcgacc      540

agcatcgcct tccggtgcat caacgagaac ctcacccgct acatcagcaa catggacatc      600

ttcgagaagg tggacgccat cttcgacaag cacgaggtcc aggagatcaa ggaaaagatc      660

ctgaactcgg actacgacgt ggaagacttc tttgagggcg agttcttcaa cttcgtcctc      720

acccaggagg gcatcgacgt ctacaacgcc atcatcggcg gcttcgtgac ggagagcggc      780

gagaagatca agggcctcaa cgagtacatc aacctctaca accagaagac taagcagaag      840

ctcccgaagt tcaagccgct gtacaagcaa gtcctgagcg accgggagtc cctctcgttc      900

tacggcgagg gctacacgag cgacgaggag gtgctggagg tgttccgcaa cacgctgaac      960

aagaacagcg agatcttcag ctcgatcaag aaactcgaga agctgttcaa gaacttcgac     1020

gagtacagca gcgccggcat cttcgtcaag aacgggcccg cgatcagcac catcagcaag     1080

gacatcttcg gggagtggaa cgtgatccgc gacaagtgga acgccgagta cgacgacatc     1140

cacctcaaga aaaaggcggt ggtcacggag aagtacgagg acgaccgccg gaagtccttc     1200

aagaaaatcg ggagcttcag cctcgagcag ctccaggagt acgcggacgc cgacctgagc     1260

gtggtggaga agctcaagga gatcatcatc cagaaggtcg acgagatcta caaggtctac     1320

ggctcgagcg agaagctgtt cgacgcggac ttcgtgctgg agaagtccct caagaagaac     1380

gacgccgtgg tggccatcat gaaggatctg ctcgacagcg tgaagtcgtt cgagaactac     1440

atcaaggcat tctttgggga gggcaaggag acgaaccggg acgagtcctt ctacggggac     1500

ttcgtgctcg cgtacgacat cctcctgaag gtcgaccaca tctacgacgc gatccggaac     1560

tacgtcacgc agaagcccta cagcaaggac aagttcaagc tctacttcca gaacccgcag     1620

ttcatgggcg ggtgggacaa ggacaaggag accgactacc gggccacgat cctgcggtac     1680

gggtccaagt actacctcgc catcatggac aagaagtacg ccaagtgcct ccagaagatt     1740

gacaaggacg acgtgaacgg gaactacgag aagatcaact acaagctcct cccggggccc     1800

aacaagatgc tgccgaaggt gttcttcagc aagaagtgga tggcctacta caacccctcg     1860

gaggacatcc agaagatata caagaacggc acgttcaaaa agggggacat gttcaacctg     1920

aacgactgcc acaagctgat cgactttttc aaggacagca tcagccgcta cccgaagtgg     1980

tcgaacgcct acgacttcaa cttctcggag acggagaagt acaaggacat tgcgggcttc     2040

taccgggagg tggaggagca gggctacaag gtctccttcg agagcgcctc caagaaagag     2100

gtggacaagc tcgtggagga gggcaagctg tacatgttcc agatctacaa caaggacttc     2160

tcggacaagt cgcacggcac cccgaacctc cacacgatgt acttcaagct gctgttcgac     2220

gagaacaacc acgggcagat ccgcctcagc ggcggggcgg agctgttcat gcgccgcgcg     2280

tccctcaaga aggaggagct ggtcgtgcac cccgccaact ccccgatcgc gaacaagaac     2340

cccgacaacc ccaagaagac aaccaccctc tcgtacgacg tctacaagga caagcggttc     2400

tcggaggacc agtacgagct gcacatcccg atcgccatca acaagtgccc caagaacatc     2460

ttcaagatca acaccgaggt gcgggtgctg ctcaagcacg acgacaaccc ctacgtcatc     2520

gggatcgacc gcggcgagcg gaacctgctc tacatcgtgg tcgtggacgg gaaggggaac     2580

atcgtggagc agtacagcct gaacgagatc atcaacaact tcaacggcat ccgcatcaag     2640

acggactacc acagcctcct ggacaagaag gagaaggagc ggttcgaggc gcggcagaac     2700

tggacctcca tcgagaacat caaggagctg aaggccggct acatcagcca ggtcgtgcac     2760

aagatctgcg agctcgtgga gaagtacgac gcggtgatcg cgctggagga cttgaacagc     2820

gggttcaaga actcccgggt caaggtcgag aagcaggtct accagaagtt cgagaagatg     2880

ctgatcgaca agctcaacta catggtggac aagaagtcca acccctgcgc caccggcggc     2940

gccctcaagg gctaccagat caccaacaag ttcgagtcct tcaagtcgat gtctacgcag     3000

aacgggttca ttttctacat cccggcgtgg ctcaccagca agatcgaccc gagcacgggc     3060

ttcgtcaacc tcctgaagac caagtacacc agcatcgcgg acagcaagaa gttcatctcc     3120

tcgttcgacc gcatcatgta cgtccccgag gaagacctgt tcgagttcgc cctcgactac     3180

aagaacttct cccggacgga cgccgactac atcaaaaagt ggaagctcta cagctacggc     3240

aaccggatcc gcatcttccg caaccccaag aagaacaatg tgttcgactg ggaggaggtg     3300

tgcctgacga gcgcctacaa ggagctcttc aacaagtacg gcatcaacta ccagcaaggg     3360

gacatccgcg cgctgctctg cgagcagtcc gacaaggcgt tctactcgtc gttcatggcc     3420

ctgatgagcc tcatgctcca gatgcgcaac agcatcaccg gccggacgga cgtggacttc     3480

ctgatcagcc cggtcaagaa cagcgacggc attttctacg acagccggaa ctacgaggcc     3540

caggagaacg ccatcctccc caagaacgcc gacgcgaacg gcgcctacaa catcgcgcgg     3600

aaggtgctgt gggccatcgg ccagtttaaa aaggcggagg acgagaagct ggacaaggtc     3660

aagatcgcca tcagcaacaa ggagtggctc gagtacgcgc agacgagcgt gaagcacgga     3720

tctaagaaga gaagaattaa acaagattga                                      3750


<210>  13
<211>  298
<212>  DNA
<213>  Zea mays


<220>
<221>  misc_feature
<222>  (1)..(298)
<223>  DNA sequence of the 3' UTR, T-Os.LTP:2.

<400>  13
taatcgatcc tccgatccct taattaccat accattacac catgcatcaa tatccatata       60

tatataaacc ctttcgcacg tacttatact atgttttgtc atacatatat atgtgtcgaa      120

cgatcgatct atcactgata tgatatgatt gatccatcag cctgatctct gtatcttgtt      180

atttgtatac cgtcaaataa aagtttcttc cacttgtgtt aataattagc tactctcatc      240

tcatgaaccc tatatataac tagtttaatt tgctgtcaat tgaacatgat gatcgatg        298


<210>  14
<211>  23
<212>  DNA
<213>  Zea mays


<220>
<221>  misc_feature
<222>  (1)..(23)
<223>  DNA sequence of a guide RNA spacer, NR-Zm.Brm3_2691.

<400>  14
cggcagcgcg tcgtagcagt tct                                               23


<210>  15
<211>  70
<212>  DNA
<213>  Artificial

<220>
<223>  DNA sequence of a guide RNA, gRNA-Zm.Brm3_2691.

<400>  15
gaatttctac taagtgtaga tcggcagcgc gtcgtagcag ttctaatttc tactaagtgt       60

agattttttt                                                              70


<210>  16
<211>  23
<212>  DNA
<213>  Zea mays


<220>
<221>  misc_feature
<222>  (1)..(23)
<223>  DNA sequence of a guide RNA spacer, NR-Zm.Brm3_3170.

<400>  16
gaatccacga catgcaagag ctc                                               23


<210>  17
<211>  70
<212>  DNA
<213>  Artificial

<220>
<223>  DNA sequence of a guide RNA, gRNA-Zm.Brm3_3170.

<400>  17
gaatttctac taagtgtaga tgaatccacg acatgcaaga gctcaatttc tactaagtgt       60

agattttttt                                                              70


<210>  18
<211>  3955
<212>  DNA
<213>  Zea mays


<220>
<221>  misc_feature
<222>  (1)..(3955)
<223>  DNA sequence of the Zea mays Brown midrib 3 (Brm3) genomic region
       targeted for genome editing.

<400>  18
ataaaaagtt gtagatctta aaaagttatt aaactttata gttagttagc aactttttcg       60

tttaaattcg tttagtgcta caaacaatcg atttatattc ggtttgtttt aatatgcgga      120

caacaaacct ataatctaaa cataagttaa agttagtgat gggtaaagtg gtagagaagt      180

gaggttagag tttcgattcg cgaaaggtcc tacaacaaac ctaaaatctt tttttgctat      240

ttattttaaa acatggttta tatttccaaa aaaatgtatt ggcggttttt aactcaggcg      300

ccaacaaaaa ttgatttcca cggcagctgc caccgtcgtt atcgctgacc aacccggctg      360

gtcgcctgtc tgctccatcc atgcatgtta caactatgca gatgcagccg aaacaaacac      420

tggctagaaa ggcagccaac gggcctactg tcattcgctc cggcatccta ctggtgggcc      480

cagttgcacc ggccgatgac cagttcatca tttttctcga cgaatttgtg cacagatcga      540

atttactaaa aattcttcgc acgtggcaaa accaggggaa aaatcgagaa ctagtcgggt      600

ttttttatat tccctgatag aatagtccct gctattcatc catgaaaacc aaacacgtac      660

actacgtcac cgtcatggat gggagcgagt gaactgatga ttttttcccc accccgcacg      720

caacagcatg ggtgacaaca accactcccg ctgcggttgg gcgagcacat ctctacgcac      780

ttgacactca cgcaaaccta acgcatacta gagtaatcat cgccaccaac tatcggcgac      840

agaaacgatg ggccccgctt ctcttaatca cggtgcttga attagtgcgc gcatagtagt      900

gaaaataata gtgaaaaata agcagtgcgt gttttggtgt ggtggttggt gagccgtccg      960

gcccaataaa aaccctcgca ccacctcgtc cctcttcgtc gcatcgcacg ccatcagcta     1020

gcagctagcg cgctcctcga gcccagcaga gaaaggccgg cagcgctaat cgtaatagcc     1080

atgggctcca ccgccggcga cgtggccgcg gtggtggacg aggaggcgtg catgtacgcg     1140

atgcagctgg cgtcgtcgtc catcctgccc atgacgctga agaacgccat cgagctgggc     1200

ctgctggagg tgctgcagaa ggaggccggc ggcggcaagg cggcgctggc gcccgaggag     1260

gtggtggcgc ggatgcccgc ggcgcccggc gaccccgccg ccgcggcggc catggtggac     1320

cgcatgctcc gcctgctcgc ctcctacgac gtcgtccggt gccagatgga ggaccgggac     1380

ggccggtacg agcgccgcta ctccgccgcg cccgtctgca agtggctcac ccccaacgag     1440

gacggcgtgt ccatggccgc cctcgcgctc atgaaccagg acaaggtcct catggagagc     1500

tggtgagtag tagccgcatc gcatcaacca ccttctacct atctatatcc atcacttgtt     1560

gctgctggcg tgcgcggcat gcatgatgac gagctcgctc atcattggtg ctactagtga     1620

tttatttcgt ccagtaaaat taattaaggt gcgctgctac tctactggct gcggctagca     1680

caaggctgga aatagttgtt acttgttata cacgatataa tatttctcta gaacaaaaag     1740

attttttttt ttataaaaag caagcaagaa agaaagtgag tgacttcatg tttttcctaa     1800

aaaaaagtta ggagtgggat ggaaaagtca gcaaggacca cttgtttgtt gtccactatc     1860

catccagtgg gtgagacttt tttgcgagac ggagcactat attattggcc gagtcctttt     1920

tctgtatccg caaaacggca gccgtcgatc gccggacgga tcgacggctc acatgagtgt     1980

cgagtccaat tccaaccacg agggcggcaa ggaaaaccat ccgtgctggt ctggactttt     2040

tgccaaactc cattcagcca ttcgccgact gaaggtgaat cttcagacag ccagattgtt     2100

tggtgtctag tgtgtgcgaa gatggcgtag aaaagactga gagacagttg gctcacacag     2160

acaagtgaca actgactata gtatctgcct gcctggctga tgctgataga gatggggact     2220

cttgtcctgt ctgtttcttg tatgcgctga tctgattctg atcactgcca ctctgccagg     2280

tactatctca aggacgcggt gctggacggc ggcatcccgt tcaacaaggc gtacgggatg     2340

acggcgttcg agtaccacgg gacggacccg cgcttcaacc gcgtgttcaa cgagggcatg     2400

aagaaccact cggtgatcat caccaagaag ctgctggact tctacacggg cttcgagggc     2460

gtgtcgacgc tggtggacgt gggcggcggc gtgggcgcca cgctgcacgc catcacgtcc     2520

cgccacccgc acatctccgg ggtcaacttc gacctgccgc acgtcatctc cgaggcgccg     2580

ccgttccccg gcgtgcgcca cgtgggcggg gacatgttcg cgtccgtgcc cgccggcgac     2640

gccatcctca tgaagtggat cctccacgac tggagcgacg cgcactgcgc cacgctgctc     2700

aagaactgct acgacgcgct gccggaaaat ggcaaggtca tcgtcgtcga gtgcgtgctg     2760

ccggtcaaca cggaggccac ccccaaggcg cagggcgtgt tccacgtcga catgatcatg     2820

ctcgcgcaca acccaggcgg caaggagcgg tacgagcgcg agttccgcga gctcgccaag     2880

ggcgccggct tctccgggtt caaggccacc tacatctacg ccaacgcctg ggccatcgag     2940

ttcatcaagt gaatacggct accaccgtcg ccgcgatgag atgcatggct gccacatgca     3000

tgcttgcttg cttggtcctc gtatcgtacg tcgccgtcgt cgtcttcttc tggttgcgct     3060

gctaccttgc tgctctcgcc ctcgcgtatg catgtacttt tgcttaattt tctttcttca     3120

tatcatgcac tctggctggc ctagactgcc cccgatctat gctggccggt acgtcttgtc     3180

gagctcttgc atgtcgtgga ttctaaattc ttcttctgcg tcgaattgtc tctgccatgt     3240

gcgagtaata acaatcaagg ttatacttac catacaatta catggcggtt taattgctct     3300

cttttaattt ggtgaagaat cgaatcgatt ctactgaatt cttctctgtc gtttaatgaa     3360

atttcctttg tcttttggat tatataatat aacacagaca ctcacaaaga cactcacaac     3420

gtgcgcacac tcattcctat aaacgcacac gcgcaaatcc tactcctatc aagtatcaac     3480

actttcaaga cttgaactgg tcataccgga gaaaatggac tttcgttcga ggacataatt     3540

acgtgttgct ttagaaccaa taccaactca atcatcaaca tcacttttta actgttagta     3600

tcggtttgtg tcttcaataa gtactaatgt taagtcatta acaccggttg aatacaccaa     3660

ctgccgaaga ttgttcgaca gtgaatatac gaggtatgtc tatgaatgaa aacggacgga     3720

aacggtcgga aaaatcaatc tcccatttcg gtttccattt tttatcaaaa acaggagcgg     3780

gagcgatata gcaagaaata gaaaaggtag tgggatatac ggttacacga aaacggatta     3840

aaacaaacgg atatgcaacg aaaatggatg ctaatcggaa actttggctg gaatatgcaa     3900

tattttataa tcactcaacg actacaatat taagagttta gaatactata aatca          3955


<210>  19
<211>  1249
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of Cas12a_NLS encoded by SEQ ID NO:12.

<400>  19

Met Ala Gly Ser Lys Lys Arg Arg Ile Lys Gln Asp Ser Lys Leu Glu 
1               5                   10                  15      


Lys Phe Thr Asn Cys Tyr Ser Leu Ser Lys Thr Leu Arg Phe Lys Ala 
            20                  25                  30          


Ile Pro Val Gly Lys Thr Gln Glu Asn Ile Asp Asn Lys Arg Leu Leu 
        35                  40                  45              


Val Glu Asp Glu Lys Arg Ala Glu Asp Tyr Lys Gly Val Lys Lys Leu 
    50                  55                  60                  


Leu Asp Arg Tyr Tyr Leu Ser Phe Ile Asn Asp Val Leu His Ser Ile 
65                  70                  75                  80  


Lys Leu Lys Asn Leu Asn Asn Tyr Ile Ser Leu Phe Arg Lys Lys Thr 
                85                  90                  95      


Arg Thr Glu Lys Glu Asn Lys Glu Leu Glu Asn Leu Glu Ile Asn Leu 
            100                 105                 110         


Arg Lys Glu Ile Ala Lys Ala Phe Lys Gly Asn Glu Gly Tyr Lys Ser 
        115                 120                 125             


Leu Phe Lys Lys Asp Ile Ile Glu Thr Ile Leu Pro Glu Phe Leu Asp 
    130                 135                 140                 


Asp Lys Asp Glu Ile Ala Leu Val Asn Ser Phe Asn Gly Phe Thr Thr 
145                 150                 155                 160 


Ala Phe Thr Gly Phe Phe Asp Asn Arg Glu Asn Met Phe Ser Glu Glu 
                165                 170                 175     


Ala Lys Ser Thr Ser Ile Ala Phe Arg Cys Ile Asn Glu Asn Leu Thr 
            180                 185                 190         


Arg Tyr Ile Ser Asn Met Asp Ile Phe Glu Lys Val Asp Ala Ile Phe 
        195                 200                 205             


Asp Lys His Glu Val Gln Glu Ile Lys Glu Lys Ile Leu Asn Ser Asp 
    210                 215                 220                 


Tyr Asp Val Glu Asp Phe Phe Glu Gly Glu Phe Phe Asn Phe Val Leu 
225                 230                 235                 240 


Thr Gln Glu Gly Ile Asp Val Tyr Asn Ala Ile Ile Gly Gly Phe Val 
                245                 250                 255     


Thr Glu Ser Gly Glu Lys Ile Lys Gly Leu Asn Glu Tyr Ile Asn Leu 
            260                 265                 270         


Tyr Asn Gln Lys Thr Lys Gln Lys Leu Pro Lys Phe Lys Pro Leu Tyr 
        275                 280                 285             


Lys Gln Val Leu Ser Asp Arg Glu Ser Leu Ser Phe Tyr Gly Glu Gly 
    290                 295                 300                 


Tyr Thr Ser Asp Glu Glu Val Leu Glu Val Phe Arg Asn Thr Leu Asn 
305                 310                 315                 320 


Lys Asn Ser Glu Ile Phe Ser Ser Ile Lys Lys Leu Glu Lys Leu Phe 
                325                 330                 335     


Lys Asn Phe Asp Glu Tyr Ser Ser Ala Gly Ile Phe Val Lys Asn Gly 
            340                 345                 350         


Pro Ala Ile Ser Thr Ile Ser Lys Asp Ile Phe Gly Glu Trp Asn Val 
        355                 360                 365             


Ile Arg Asp Lys Trp Asn Ala Glu Tyr Asp Asp Ile His Leu Lys Lys 
    370                 375                 380                 


Lys Ala Val Val Thr Glu Lys Tyr Glu Asp Asp Arg Arg Lys Ser Phe 
385                 390                 395                 400 


Lys Lys Ile Gly Ser Phe Ser Leu Glu Gln Leu Gln Glu Tyr Ala Asp 
                405                 410                 415     


Ala Asp Leu Ser Val Val Glu Lys Leu Lys Glu Ile Ile Ile Gln Lys 
            420                 425                 430         


Val Asp Glu Ile Tyr Lys Val Tyr Gly Ser Ser Glu Lys Leu Phe Asp 
        435                 440                 445             


Ala Asp Phe Val Leu Glu Lys Ser Leu Lys Lys Asn Asp Ala Val Val 
    450                 455                 460                 


Ala Ile Met Lys Asp Leu Leu Asp Ser Val Lys Ser Phe Glu Asn Tyr 
465                 470                 475                 480 


Ile Lys Ala Phe Phe Gly Glu Gly Lys Glu Thr Asn Arg Asp Glu Ser 
                485                 490                 495     


Phe Tyr Gly Asp Phe Val Leu Ala Tyr Asp Ile Leu Leu Lys Val Asp 
            500                 505                 510         


His Ile Tyr Asp Ala Ile Arg Asn Tyr Val Thr Gln Lys Pro Tyr Ser 
        515                 520                 525             


Lys Asp Lys Phe Lys Leu Tyr Phe Gln Asn Pro Gln Phe Met Gly Gly 
    530                 535                 540                 


Trp Asp Lys Asp Lys Glu Thr Asp Tyr Arg Ala Thr Ile Leu Arg Tyr 
545                 550                 555                 560 


Gly Ser Lys Tyr Tyr Leu Ala Ile Met Asp Lys Lys Tyr Ala Lys Cys 
                565                 570                 575     


Leu Gln Lys Ile Asp Lys Asp Asp Val Asn Gly Asn Tyr Glu Lys Ile 
            580                 585                 590         


Asn Tyr Lys Leu Leu Pro Gly Pro Asn Lys Met Leu Pro Lys Val Phe 
        595                 600                 605             


Phe Ser Lys Lys Trp Met Ala Tyr Tyr Asn Pro Ser Glu Asp Ile Gln 
    610                 615                 620                 


Lys Ile Tyr Lys Asn Gly Thr Phe Lys Lys Gly Asp Met Phe Asn Leu 
625                 630                 635                 640 


Asn Asp Cys His Lys Leu Ile Asp Phe Phe Lys Asp Ser Ile Ser Arg 
                645                 650                 655     


Tyr Pro Lys Trp Ser Asn Ala Tyr Asp Phe Asn Phe Ser Glu Thr Glu 
            660                 665                 670         


Lys Tyr Lys Asp Ile Ala Gly Phe Tyr Arg Glu Val Glu Glu Gln Gly 
        675                 680                 685             


Tyr Lys Val Ser Phe Glu Ser Ala Ser Lys Lys Glu Val Asp Lys Leu 
    690                 695                 700                 


Val Glu Glu Gly Lys Leu Tyr Met Phe Gln Ile Tyr Asn Lys Asp Phe 
705                 710                 715                 720 


Ser Asp Lys Ser His Gly Thr Pro Asn Leu His Thr Met Tyr Phe Lys 
                725                 730                 735     


Leu Leu Phe Asp Glu Asn Asn His Gly Gln Ile Arg Leu Ser Gly Gly 
            740                 745                 750         


Ala Glu Leu Phe Met Arg Arg Ala Ser Leu Lys Lys Glu Glu Leu Val 
        755                 760                 765             


Val His Pro Ala Asn Ser Pro Ile Ala Asn Lys Asn Pro Asp Asn Pro 
    770                 775                 780                 


Lys Lys Thr Thr Thr Leu Ser Tyr Asp Val Tyr Lys Asp Lys Arg Phe 
785                 790                 795                 800 


Ser Glu Asp Gln Tyr Glu Leu His Ile Pro Ile Ala Ile Asn Lys Cys 
                805                 810                 815     


Pro Lys Asn Ile Phe Lys Ile Asn Thr Glu Val Arg Val Leu Leu Lys 
            820                 825                 830         


His Asp Asp Asn Pro Tyr Val Ile Gly Ile Asp Arg Gly Glu Arg Asn 
        835                 840                 845             


Leu Leu Tyr Ile Val Val Val Asp Gly Lys Gly Asn Ile Val Glu Gln 
    850                 855                 860                 


Tyr Ser Leu Asn Glu Ile Ile Asn Asn Phe Asn Gly Ile Arg Ile Lys 
865                 870                 875                 880 


Thr Asp Tyr His Ser Leu Leu Asp Lys Lys Glu Lys Glu Arg Phe Glu 
                885                 890                 895     


Ala Arg Gln Asn Trp Thr Ser Ile Glu Asn Ile Lys Glu Leu Lys Ala 
            900                 905                 910         


Gly Tyr Ile Ser Gln Val Val His Lys Ile Cys Glu Leu Val Glu Lys 
        915                 920                 925             


Tyr Asp Ala Val Ile Ala Leu Glu Asp Leu Asn Ser Gly Phe Lys Asn 
    930                 935                 940                 


Ser Arg Val Lys Val Glu Lys Gln Val Tyr Gln Lys Phe Glu Lys Met 
945                 950                 955                 960 


Leu Ile Asp Lys Leu Asn Tyr Met Val Asp Lys Lys Ser Asn Pro Cys 
                965                 970                 975     


Ala Thr Gly Gly Ala Leu Lys Gly Tyr Gln Ile Thr Asn Lys Phe Glu 
            980                 985                 990         


Ser Phe Lys Ser Met Ser Thr Gln  Asn Gly Phe Ile Phe  Tyr Ile Pro 
        995                 1000                 1005             


Ala Trp  Leu Thr Ser Lys Ile  Asp Pro Ser Thr Gly  Phe Val Asn 
    1010                 1015                 1020             


Leu Leu  Lys Thr Lys Tyr Thr  Ser Ile Ala Asp Ser  Lys Lys Phe 
    1025                 1030                 1035             


Ile Ser  Ser Phe Asp Arg Ile  Met Tyr Val Pro Glu  Glu Asp Leu 
    1040                 1045                 1050             


Phe Glu  Phe Ala Leu Asp Tyr  Lys Asn Phe Ser Arg  Thr Asp Ala 
    1055                 1060                 1065             


Asp Tyr  Ile Lys Lys Trp Lys  Leu Tyr Ser Tyr Gly  Asn Arg Ile 
    1070                 1075                 1080             


Arg Ile  Phe Arg Asn Pro Lys  Lys Asn Asn Val Phe  Asp Trp Glu 
    1085                 1090                 1095             


Glu Val  Cys Leu Thr Ser Ala  Tyr Lys Glu Leu Phe  Asn Lys Tyr 
    1100                 1105                 1110             


Gly Ile  Asn Tyr Gln Gln Gly  Asp Ile Arg Ala Leu  Leu Cys Glu 
    1115                 1120                 1125             


Gln Ser  Asp Lys Ala Phe Tyr  Ser Ser Phe Met Ala  Leu Met Ser 
    1130                 1135                 1140             


Leu Met  Leu Gln Met Arg Asn  Ser Ile Thr Gly Arg  Thr Asp Val 
    1145                 1150                 1155             


Asp Phe  Leu Ile Ser Pro Val  Lys Asn Ser Asp Gly  Ile Phe Tyr 
    1160                 1165                 1170             


Asp Ser  Arg Asn Tyr Glu Ala  Gln Glu Asn Ala Ile  Leu Pro Lys 
    1175                 1180                 1185             


Asn Ala  Asp Ala Asn Gly Ala  Tyr Asn Ile Ala Arg  Lys Val Leu 
    1190                 1195                 1200             


Trp Ala  Ile Gly Gln Phe Lys  Lys Ala Glu Asp Glu  Lys Leu Asp 
    1205                 1210                 1215             


Lys Val  Lys Ile Ala Ile Ser  Asn Lys Glu Trp Leu  Glu Tyr Ala 
    1220                 1225                 1230             


Gln Thr  Ser Val Lys His Gly  Ser Lys Lys Arg Arg  Ile Lys Gln 
    1235                 1240                 1245             


Asp 
    


<210>  20
<211>  23
<212>  DNA
<213>  Zea mays


<220>
<221>  misc_feature
<222>  (1)..(23)
<223>  DNA sequence of a guide RNA spacer, NR-Zm.Bmr3_90.

<400>  20
ggctgcatct gcatagttgt aac                                               23


<210>  21
<211>  23
<212>  DNA
<213>  Zea mays


<220>
<221>  misc_feature
<222>  (1)..(23)
<223>  DNA sequence of a guide RNA spacer, NR-Zm.Bmr3_227.

<400>  21
gtaaattcga tctgtgcaca aat                                               23


<210>  22
<211>  23
<212>  DNA
<213>  Zea mays


<220>
<221>  misc_feature
<222>  (1)..(23)
<223>  DNA sequence of a guide RNA spacer, NR-Zm.Brm3_3279.

<400>  22
gtgaagaatc gaatcgattc tac                                               23


<210>  23
<211>  114
<212>  DNA
<213>  Artificial

<220>
<223>  DNA sequence of a guide RNA, gRNA-Zm.Bmr3_90_3279.

<400>  23
gaatttctac taagtgtaga tggctgcatc tgcatagttg taacaatttc tactaagtgt       60

agatgtgaag aatcgaatcg attctacaat ttctactaag tgtagatttt tttt            114


<210>  24
<211>  114
<212>  DNA
<213>  Artificial

<220>
<223>  DNA sequence of a guide RNA, gRNA-Zm.Bmr3_227_3279.

<400>  24
gaatttctac taagtgtaga tgtaaattcg atctgtgcac aaataatttc tactaagtgt       60

agatgtgaag aatcgaatcg attctacaat ttctactaag tgtagatttt tttt            114


<210>  25
<211>  51
<212>  DNA
<213>  Artificial

<220>
<223>  DNA sequence of a guide RNA, gRNA-Zm.Brm3_2691_2.

<400>  25
gaatttctac taagtgtaga tcggcagcgc gtcgtagcag ttcttttttt t                51


<210>  26
<211>  51
<212>  DNA
<213>  Artificial

<220>
<223>  DNA sequence of a guide RNA, gRNA-Zm.Brm3_3170_2.

<400>  26
gaatttctac taagtgtaga tgaatccacg acatgcaaga gctctttttt t                51


<210>  27
<211>  94
<212>  DNA
<213>  Artificial

<220>
<223>  DNA sequence of a guide RNA, gRNA-Zm.Brm3_2691_3170.

<400>  27
gaatttctac taagtgtaga tcggcagcgc gtcgtagcag ttctaatttc tactaagtgt       60

agatgaatcc acgacatgca agagctcttt tttt                                   94


<210>  28
<211>  476
<212>  DNA
<213>  Zea mays


<220>
<221>  misc_feature
<222>  (1)..(476)
<223>  DNA sequence of the Zea mays Zm7 genomic region targeted for 
       genome editing.

<400>  28
gtcaagagca agcaacgaat atgctgtgct actcctctac gtgcggagac gtgttgtatt       60

ttattctatg agcgttattt actgacgtaa ggtatggttt aatcgacgga ttgctatcct      120

ggtcgggctt aattaaggac gttgaagcct ggtaaggtat actaacttag cgtccttcgg      180

aatgggagtg agttgggagg acttgaaaat gtaagaagaa gaaaacaaag catgtgatgg      240

atgcttctgg atgtgcaagc tcagtcttca ttcttcagca gcgcggaaat tggctctaat      300

aacattggtt tatgttgaca gtgctagcga ggacaatgag tcattcgatg tgaaataata      360

ccatctgttc tttatgatag tctatttagt ataatatgat ggcatgccct cttaggcagt      420

agccggccag catttgaaac atggtacttc gttttattta aaaaataata agttat          476


<210>  29
<211>  23
<212>  DNA
<213>  Zea mays


<220>
<221>  misc_feature
<222>  (1)..(23)
<223>  DNA sequence of a guide RNA spacer, NR-Zm.7.1b.

<400>  29
ctgacgtaag gtatggttta atc                                               23


<210>  30
<211>  51
<212>  DNA
<213>  Artificial

<220>
<223>  DNA sequence of a guide RNA, gRNA-Zm.7.1b.

<400>  30
gaatttctac taagtgtaga tctgacgtaa ggtatggttt aatctttttt t                51


<210>  31
<211>  23
<212>  DNA
<213>  Zea mays


<220>
<221>  misc_feature
<222>  (1)..(23)
<223>  DNA sequence of a guide RNA spacer, NR-Zm.7.1c.

<400>  31
gtataatatg atggcatgcc ctc                                               23


<210>  32
<211>  51
<212>  DNA
<213>  Artificial

<220>
<223>  DNA sequence of a guide RNA, gRNA-Zm.7.1c.

<400>  32
gaatttctac taagtgtaga tgtataatat gatggcatgc cctctttttt t                51


<210>  33
<211>  94
<212>  DNA
<213>  Artificial

<220>
<223>  DNA sequence of a guide RNA, gRNA-7.1c_7.1b.

<400>  33
gaatttctac taagtgtaga tgtataatat gatggcatgc cctcaatttc tactaagtgt       60

agatctgacg taaggtatgg tttaatcttt tttt                                   94


