                         SEQUENCE LISTING

<110>  Massachusetts Institute of Technology, Nanyang 
       Technological University and National University of Singapore
       Nanyang Technological University
       National University of Singapore
 
<120>  Methods of Detecting Bacterial/Fungal Contamination In A 
       Therapeutic Product

<130>  S61016451

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  213
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C. albicans ATCC 10231

<400>  1

Met Lys Lys Thr Ala Leu Ile Val Val Asp Leu Gln Glu Asp Phe Leu 
1               5                   10                  15      


Pro Pro Asn Gly Ser Leu Ala Ile Lys Asn Gly Arg Ser Val Ile Pro 
            20                  25                  30          


Lys Ile Asn Gln Leu Leu Pro Ser Gln Asp Asn His Ser Lys Phe Asp 
        35                  40                  45              


Trp Ser Leu Ile Val Ala Thr Gln Asp Trp His Pro Pro Asn His Thr 
    50                  55                  60                  


Ser Phe Ala Ser Gln His Glu Asn Val Ala Pro Phe Thr Glu Ile Glu 
65                  70                  75                  80  


Phe Ile His Pro Glu Lys Lys Leu Asp Pro Lys Thr Asn Gln Pro Ile 
                85                  90                  95      


Val Met Asn Gln Ile Val Trp Pro Asp His Cys Val Gln Gly Thr Lys 
            100                 105                 110         


Gly Ala Gln Leu Glu Pro Leu Phe Ala Asn Gln Phe Glu Lys Leu Thr 
        115                 120                 125             


Lys Gln Asp Asp Asn Asn Thr Ala Pro Cys Lys Ile Val Lys Lys Gly 
    130                 135                 140                 


Tyr Leu Pro Asp Arg Glu Tyr Tyr Ser Cys Phe Gln Asp Cys Trp Gly 
145                 150                 155                 160 


Leu His His Thr Glu Leu Ile Asp Leu Leu His Glu Tyr Asp Ile Glu 
                165                 170                 175     


Asn Val Val Phe Val Gly Leu Ala Tyr Asp Phe Cys Val Leu Arg Ser 
            180                 185                 190         


Ala Ile Asp Ser Ala Lys Asn Gly Phe Lys Thr Phe Val Leu Lys Asn 
        195                 200                 205             


Tyr Cys Glu Ser Val 
    210             


<210>  2
<211>  186
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  S. areus ATCC 6538

<400>  2

Met Thr His Arg Ala Leu Leu Val Val Asp Tyr Ser Tyr Asp Phe Ile 
1               5                   10                  15      


Ala Asp Asp Gly Leu Leu Thr Cys Gly Lys Pro Gly Gln Asn Ile Glu 
            20                  25                  30          


Asp Phe Ile Val Ser Arg Ile Asn Asp Phe Asn Tyr Tyr Gln Asp His 
        35                  40                  45              


Ile Phe Phe Leu Met Asp Leu His Tyr Leu His Asp Ile His His Pro 
    50                  55                  60                  


Glu Ser Lys Leu Phe Pro Pro His Asn Ile Val Asp Thr Ser Gly Arg 
65                  70                  75                  80  


Glu Leu Tyr Gly Lys Val Gly Lys Leu Tyr Glu Thr Ile Lys Ala Gln 
                85                  90                  95      


Pro Asn Val His Phe Ile Asp Lys Thr Arg Tyr Asp Ser Phe Phe Gly 
            100                 105                 110         


Thr Pro Leu Asp Ser Leu Leu Arg Glu Arg Ser Ile Asn Gln Val Glu 
        115                 120                 125             


Ile Val Gly Val Cys Thr Asp Ile Cys Val Leu His Thr Ala Ile Ser 
    130                 135                 140                 


Ala Tyr Asn Leu Gly Tyr Lys Ile Ser Val Pro Ala Glu Gly Val Ala 
145                 150                 155                 160 


Ser Phe Asn Gln Lys Gly His Glu Trp Ala Leu Ala His Phe Lys Asn 
                165                 170                 175     


Ser Leu Gly Ala Glu Val Glu Gln His Val 
            180                 185     


<210>  3
<211>  183
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B. subtilis ATCC 6633

<400>  3

Met Lys Lys Ala Leu Ile Cys Ile Asp Tyr Thr Asn Asp Phe Val Ala 
1               5                   10                  15      


Ser Asp Gly Lys Leu Thr Cys Gly Glu Pro Gly Arg Met Ile Glu Glu 
            20                  25                  30          


Ala Ile Val Asn Leu Thr Glu Glu Phe Ile Thr Asn Gly Asp Tyr Val 
        35                  40                  45              


Val Leu Ala Val Asp Ser His Asp Glu Gly Asp Gln Tyr His Pro Glu 
    50                  55                  60                  


Thr Arg Leu Phe Pro Pro His Asn Ile Lys Gly Thr Asp Gly Lys Asp 
65                  70                  75                  80  


Leu Tyr Gly Lys Leu Leu Pro Leu Tyr Gln Lys His Glu His Glu Pro 
                85                  90                  95      


Asn Val Tyr Tyr Met Glu Lys Thr Arg Tyr Ser Ala Phe Ala Gly Thr 
            100                 105                 110         


Asp Leu Glu Leu Lys Leu Arg Glu Arg Gln Ile Asp Glu Leu His Leu 
        115                 120                 125             


Ala Gly Val Cys Thr Asp Ile Cys Val Leu His Thr Ala Val Asp Ala 
    130                 135                 140                 


Tyr Asn Lys Gly Phe Arg Ile Val Val His Lys Gln Ala Val Ala Ser 
145                 150                 155                 160 


Phe Asn Gln Glu Gly His Thr Trp Ala Leu Ser His Phe Ala Asn Ser 
                165                 170                 175     


Ile Gly Ala Gln Val Ala Glu 
            180             


<210>  4
<211>  218
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P. aeruginosa ATCC 9027

<400>  4

Met Asn Arg Pro Lys Ile Ala Ser Phe Asp Val Asp Ala Gln Lys Gly 
1               5                   10                  15      


Phe Thr Pro Leu Cys Pro Asp Glu Leu Pro Val Pro Gly Gly Asp Ala 
            20                  25                  30          


Ile Gly Ala Ala Leu Asn Leu Leu Ala Ser Arg Ala Ala Leu Arg Leu 
        35                  40                  45              


Gly Ser Lys Asp Ala His Ser Pro Lys Ala Ala Trp Val Val Glu Ser 
    50                  55                  60                  


Ala Ala Gln Met Leu Arg Pro Leu Ala Tyr Pro Asp Ala Asp Leu Thr 
65                  70                  75                  80  


Trp Val Ala His Cys Val Pro Gly Thr Pro Gly Phe Glu Leu Leu Asp 
                85                  90                  95      


Glu Leu Pro Arg Pro Ile Asp Tyr Asp Phe Phe Val Trp Lys Gly Val 
            100                 105                 110         


Glu Pro Asp Leu His Pro Tyr Gly Ala Cys Tyr His Asp Leu Ala Glu 
        115                 120                 125             


Arg Arg Ser Thr Gly Val Ile Glu Tyr Leu Arg Gln Asn Gly Val Ser 
    130                 135                 140                 


His Val Leu Val Gly Gly Leu Ala Leu Asp Tyr Cys Val Lys Asn Thr 
145                 150                 155                 160 


Ala Leu Gln Leu Arg Arg Ala Gly Phe Glu Val Leu Leu Tyr Leu Pro 
                165                 170                 175     


Ala Cys Arg Ala Ile Ala Glu Asp Thr Ala Gln Arg Ala Cys Asp Glu 
            180                 185                 190         


Met Arg Asp Ala Gly Val Ile Leu Cys Ala Asp Leu Glu Arg Leu Asp 
        195                 200                 205             


Ser Ala Leu Ala Gly Ala Asp Lys Glu Lys 
    210                 215             


<210>  5
<211>  224
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A. brasiliensis ATCC 16404

<400>  5

Met Lys Ala Ala Leu Ile Ile Val Asp Met Gln Glu Asp Phe Cys Pro 
1               5                   10                  15      


Gln Asn Gly Ser Leu Ala Val Gln Glu Ala Arg Asp Leu Ala Pro Thr 
            20                  25                  30          


Ile Asn Thr Leu Leu Ala Thr Pro Gly Phe Thr Leu Arg Ile Ala Ser 
        35                  40                  45              


Gln Asp Ser His Pro Pro Asn His Ile Ser Phe Ala Pro Asn His Pro 
    50                  55                  60                  


Pro Pro Asn Asn Leu Pro Phe Glu His Phe Ile Glu Met Ser Asn Pro 
65                  70                  75                  80  


Ala Ala Gly Lys Glu Thr Glu Thr Lys Pro Gln Arg Leu Trp Pro Val 
                85                  90                  95      


His Cys Val Glu Gly Thr Lys Gly Ala Glu Ile Ile Pro Glu Ile Asp 
            100                 105                 110         


Thr Lys Asn Ile Asp Leu Tyr Val Arg Lys Gly Met Asp Ala Arg Val 
        115                 120                 125             


Glu Met Tyr Ser Ala Phe Ala Asp Ala Phe Gly Asn Leu Asp Ala Glu 
    130                 135                 140                 


Val Asn Arg Ser Ser Val Asp Val His Leu Lys Gly Ala Leu Glu Asp 
145                 150                 155                 160 


Lys Gly Ile Thr Asp Val Phe Cys Val Gly Val Ala Gly Asp Tyr Cys 
                165                 170                 175     


Val Lys Phe Thr Ala Ile Asp Ala Ala Arg Ala Gly Leu Arg Ser Tyr 
            180                 185                 190         


Phe Val Glu Asp Ala Val Arg Cys Val Asp Pro Gly Gln Gly Trp Glu 
        195                 200                 205             


Glu Ala Lys Arg Glu Leu Lys Glu Ala Gly Val Val Ile Val Arg Ser 
    210                 215                 220                 


<210>  6
<211>  186
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C. sporogenes ATCC 15579

<400>  6

Met Phe Lys Ser Ile Leu Ile Ile Asp Cys Gln Asn Asp Phe Ile Ser 
1               5                   10                  15      


Gly Ser Leu Ala Cys Asp Asn Gly Glu Leu Ala Val Lys Asn Ile Ile 
            20                  25                  30          


Asn Leu Leu Asn Ile Asn Lys Asp Leu Lys Val Phe Tyr Ser Leu Asp 
        35                  40                  45              


Trp His Ser Glu Asn Asn Lys Ser Phe Lys Ile Asn Gly Gly Ile Trp 
    50                  55                  60                  


Pro Val His Cys Val Ala Asp Arg Phe Gly Ala Lys Leu Glu Lys Ser 
65                  70                  75                  80  


Phe Tyr Lys Glu Leu Gln His Lys Asn His Ser Pro Asn Asn Lys Lys 
                85                  90                  95      


Ile Leu Phe Ser Lys Gly Lys Asn Asp Tyr Ile Glu Glu Tyr Ser Ala 
            100                 105                 110         


Tyr Glu Gly Glu Asn Ile Leu Gly Asn Lys Ile Asn Glu Val Leu Asp 
        115                 120                 125             


Lys Asp Val Ile Val Cys Gly Ile Ala Ser Glu Phe Cys Val Lys Glu 
    130                 135                 140                 


Thr Val Glu Asp Leu Ile Arg Asn Gly Phe Asn Val Glu Leu Tyr Val 
145                 150                 155                 160 


Asp Gly Val Gly Tyr Val Asn Lys Lys Glu His Glu Lys Thr Leu Asn 
                165                 170                 175     


Asn Leu Arg Lys Gln Gly Ala Lys Phe Ile 
            180                 185     


<210>  7
<211>  213
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PNCA_ECOLI

<400>  7

Met Pro Pro Arg Ala Leu Leu Leu Val Asp Leu Gln Asn Asp Phe Cys 
1               5                   10                  15      


Ala Gly Gly Ala Leu Ala Val Pro Glu Gly Asp Ser Thr Val Asp Val 
            20                  25                  30          


Ala Asn Arg Leu Ile Asp Trp Cys Gln Ser Arg Gly Glu Ala Val Ile 
        35                  40                  45              


Ala Ser Gln Asp Trp His Pro Ala Asn His Gly Ser Phe Ala Ser Gln 
    50                  55                  60                  


His Gly Val Glu Pro Tyr Thr Pro Gly Gln Leu Asp Gly Leu Pro Gln 
65                  70                  75                  80  


Thr Phe Trp Pro Asp His Cys Val Gln Asn Ser Glu Gly Ala Gln Leu 
                85                  90                  95      


His Pro Leu Leu His Gln Lys Ala Ile Ala Ala Val Phe His Lys Gly 
            100                 105                 110         


Glu Asn Pro Leu Val Asp Ser Tyr Ser Ala Phe Phe Asp Asn Gly Arg 
        115                 120                 125             


Arg Gln Lys Thr Ser Leu Asp Asp Trp Leu Arg Asp His Glu Ile Asp 
    130                 135                 140                 


Glu Leu Ile Val Met Gly Leu Ala Thr Asp Tyr Cys Val Lys Phe Thr 
145                 150                 155                 160 


Val Leu Asp Ala Leu Gln Leu Gly Tyr Lys Val Asn Val Ile Thr Asp 
                165                 170                 175     


Gly Cys Arg Gly Val Asn Ile Gln Pro Gln Asp Ser Ala His Ala Phe 
            180                 185                 190         


Met Glu Met Ser Ala Ala Gly Ala Thr Leu Tyr Thr Leu Ala Asp Trp 
        195                 200                 205             


Glu Glu Thr Gln Gly 
    210             


<210>  8
<211>  186
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PNCA_MYCTU

<400>  8

Met Arg Ala Leu Ile Ile Val Asp Val Gln Asn Asp Phe Cys Glu Gly 
1               5                   10                  15      


Gly Ser Leu Ala Val Thr Gly Gly Ala Ala Leu Ala Arg Ala Ile Ser 
            20                  25                  30          


Asp Tyr Leu Ala Glu Ala Ala Asp Tyr His His Val Val Ala Thr Lys 
        35                  40                  45              


Asp Phe His Ile Asp Pro Gly Asp His Phe Ser Gly Thr Pro Asp Tyr 
    50                  55                  60                  


Ser Ser Ser Trp Pro Pro His Cys Val Ser Gly Thr Pro Gly Ala Asp 
65                  70                  75                  80  


Phe His Pro Ser Leu Asp Thr Ser Ala Ile Glu Ala Val Phe Tyr Lys 
                85                  90                  95      


Gly Ala Tyr Thr Gly Ala Tyr Ser Gly Phe Glu Gly Val Asp Glu Asn 
            100                 105                 110         


Gly Thr Pro Leu Leu Asn Trp Leu Arg Gln Arg Gly Val Asp Glu Val 
        115                 120                 125             


Asp Val Val Gly Ile Ala Thr Asp His Cys Val Arg Gln Thr Ala Glu 
    130                 135                 140                 


Asp Ala Val Arg Asn Gly Leu Ala Thr Arg Val Leu Val Asp Leu Thr 
145                 150                 155                 160 


Ala Gly Val Ser Ala Asp Thr Thr Val Ala Ala Leu Glu Glu Met Arg 
                165                 170                 175     


Thr Ala Ser Val Glu Leu Val Cys Ser Ser 
            180                 185     


<210>  9
<211>  216
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PNC1_YEAST

<400>  9

Met Lys Thr Leu Ile Val Val Asp Met Gln Asn Asp Phe Ile Ser Pro 
1               5                   10                  15      


Leu Gly Ser Leu Thr Val Pro Lys Gly Glu Glu Leu Ile Asn Pro Ile 
            20                  25                  30          


Ser Asp Leu Met Gln Asp Ala Asp Arg Asp Trp His Arg Ile Val Val 
        35                  40                  45              


Thr Arg Asp Trp His Pro Ser Arg His Ile Ser Phe Ala Lys Asn His 
    50                  55                  60                  


Lys Asp Lys Glu Pro Tyr Ser Thr Tyr Thr Tyr His Ser Pro Arg Pro 
65                  70                  75                  80  


Gly Asp Asp Ser Thr Gln Glu Gly Ile Leu Trp Pro Val His Cys Val 
                85                  90                  95      


Lys Asn Thr Trp Gly Ser Gln Leu Val Asp Gln Ile Met Asp Gln Val 
            100                 105                 110         


Val Thr Lys His Ile Lys Ile Val Asp Lys Gly Phe Leu Thr Asp Arg 
        115                 120                 125             


Glu Tyr Tyr Ser Ala Phe His Asp Ile Trp Asn Phe His Lys Thr Asp 
    130                 135                 140                 


Met Asn Lys Tyr Leu Glu Lys His His Thr Asp Glu Val Tyr Ile Val 
145                 150                 155                 160 


Gly Val Ala Leu Glu Tyr Cys Val Lys Ala Thr Ala Ile Ser Ala Ala 
                165                 170                 175     


Glu Leu Gly Tyr Lys Thr Thr Val Leu Leu Asp Tyr Thr Arg Pro Ile 
            180                 185                 190         


Ser Asp Asp Pro Glu Val Ile Asn Lys Val Lys Glu Glu Leu Lys Ala 
        195                 200                 205             


His Asn Ile Asn Val Val Asp Lys 
    210                 215     


