Sequence Listing 
<110>University of the Philippines Manila

<120>Antipeptide immunoglobulins recognizing nonglycosylatedepitopes within the amino-terminal extramembranous domain5 of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) membrane glycoprotein (M protein) and cognateoligopeptide-based immunogenic constructs, methods forproducing and utilizing the same, and systems relatingthereto for the detection of antigens comprising said10 epitopes

<130>01-00004

<160>415 

<170>PatentIn version 3.5

<210>1
<211>22220 
<212>PRT
<213>Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

<300> 25 
<301>Wu F, Zhao S, Yu B, Chen YM, Wang W, Song ZG, Hu Y, TaoZW, Tian JH, Pei YY, Yuan ML, Zhang YL, Dai FH, Liu Y,Wang QM, Zheng JJ, Xu L, Holmes EC, Zhang YZ
<302>A new coronavirus associated with human respiratorydisease in China30 
<303>Nature
<304>579
<305>7798
<306>265-269
<307>2020-02-0335 
<313>(1)..(222)

<400>1

Met Ala Asp Ser Asn Gly Thr Ile Thr Val Glu Glu Leu Lys Lys Leu 40 1                  5                       10                     15 


Leu Glu Gln Trp Asn Leu Val Ile Gly Phe Leu Phe Leu Thr Trp Ile 
 20                     25                     30 45 


Cys Leu Leu Gln Phe Ala Tyr Ala Asn Arg Asn Arg Phe Leu Tyr Ile 
    35           40                               45 
50 
 
Ile Lys Leu Ile Phe Leu Trp Leu Leu Trp Pro Val Thr Leu Ala Cys 
     50                     55                      60 
 
 5 
Phe Val Leu Ala Ala Val Tyr Arg Ile Asn Trp Ile Thr Gly Gly Ile 
65                      70                     75                     80 
 
 
Ala Ile Ala Met Ala Cys Leu Val Gly Leu Met Trp Leu Ser Tyr Phe 10 
                   85                      90                     95 
 
 
Ile Ala Ser Phe Arg Leu Phe Ala Arg Thr Arg Ser Met Trp Ser Phe 
              100                    105                    110 15 
 
 
Asn Pro Glu Thr Asn Ile Leu Leu Asn Val Pro Leu His Gly Thr Ile 
         115                     120                    125 
 20 
 
Leu Thr Arg Pro Leu Leu Glu Ser Glu Leu Val Ile Gly Ala Val Ile  
     130                    135                    140 
 
 25 
Leu Arg Gly His Leu Arg Ile Ala Gly His His Leu Gly Arg Cys Asp 
145                    150                    155                     160 
 
 
Ile Lys Asp Leu Pro Lys Glu Ile Thr Val Ala Thr Ser Arg Thr Leu  30 
                   165                    170                    175 
 
 
Ser Tyr Tyr Lys Leu Gly Ala Ser Gln Arg Val Ala Gly Asp Ser Gly 
              180                     185                   190 35 
  
 
Phe Ala Ala Tyr Ser Arg Tyr Arg Ile Gly Asn Tyr Lys Leu Asn Thr 
          195                    200                    205 
 40 
 
Asp His Ser Ser Ser Ser Asp Asn Ile Ala Leu Leu Val Gln  
     210                    215                    220 
 
 45 
<210>  2 
<211>  20 
<212>  PRT 
<213>  Artificial Sequence 
 50 
<220> 
<223>  SARS-CoV-2 membrane glycoprotein sequence residues 1-19 with C-terminal Cys residue, amidated 
 
<220> 
<221>  MOD_RES 5 
<222>  (20)..(20) 
<223>  AMIDATION 
 
<400>  2 
 10 
Met Ala Asp Ser Asn Gly Thr Ile Thr Val Glu Glu Leu Lys Lys Leu 
1                  5                       10                     15 
 
Leu Glu Gln Cys 
               20 15 
 
<210>  3 
<211>  20 
<212>  PRT 
<213>  Artificial Sequence 20 
 
<220> 
<223>  SARS-CoV-2 membrane glycoprotein sequence residues 2-19 with oxidized flanking Cys residues linked via interchain disulfide bonds, amidated 25 
 
<220> 
<221>  DISULFID 
<222>  (1)..(20) 
<223>  interchain disulfide bond 30 
 
<220> 
<221>  MOD_RES 
<222>  (20)..(20) 
<223>  AMIDATION 35 
 
<400>  3 
 
Cys Ala Asp Ser Asn Gly Thr Ile Thr Val Glu Glu Leu Lys Lys Leu 
1                  5                       10                     15 40 
 
Leu Glu Gln Cys 
               20 
 
<210>  4 45 
<211>  15 
<212>  PRT 
<213>  Artificial Sequence 
 
<220> 50 
<223>  SARS-CoV-2 membrane glycoprotein sequence residues 7-19 with oxidized flanking Cys residues linked via interchain disulfide bonds, amidated 
 
 
<220> 5 
<221>  DISULFID 
<222>  (1)..(15) 
<223>  interchain disulfide bond 
 
<220> 10 
<221>  MOD_RES 
<222>  (15)..(15) 
<223>  AMIDATION 
 
<400>  4 15 
 
Cys Thr Ile Thr Val Glu Glu Leu Lys Lys Leu Leu Glu Gln Cys 
1                  5                      10                     15 
 

