               SEQUENCE LISTING

<110> Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas, O.A., M.P. (CIEMAT)
      Consorcio Centro de Investigación Biomédica en Red, M.P. (CIBER)
      Fundación Instituto de Investigación Sanitaria Fundación
      Jiménez Díaz (FIIS-FJD)

<120> IN VIVO LENTIVIRAL GENE THERAPY FOR THE TREATMENT OF PRIMARY HYPEROXALURIA TYPE 1

<130> 907 023

<150> EP21 382 363.6
<151> 2021-04-26

<160> 52

<170> BiSSAP 1.3.6

<210> 1
<211> 30
<212> DNA
<213> Artificial Sequence


<220> 
<223> forward

<400> 1
attggctagc atggtgagca agggcgagga                                     30


<210> 2
<211> 30
<212> DNA
<213> Artificial Sequence


<220> 
<223> Reverse

<400> 2
cattgtcgac ttacttgtac agctcgtcca                                     30


<210> 3
<211> 23
<212> RNA
<213> Artificial Sequence


<220> 
<223> mature human microRNA 142-3p

<400> 3
uguaguguuu ccuacuuuau gga                                            23


<210> 4
<211> 856
<212> DNA
<213> Artificial Sequence


<220> 
<223> 5’ LTR

<400> 4
tggccattgc atacgttgta tccatatcat aatatgtaca tttatattgg ctcatgtcca      60

acattaccgc catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg     120

tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg     180

cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata     240

gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc     300

cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac     360

ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg     420

cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc     480

aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc     540

aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc     600

gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct     660

cgtttagtga accggggtct ctctggttag accagatctg agcctgggag ctctctggct     720

aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt     780

gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt     840

ggaaaatctc tagcag                                                     856


<210> 5
<211> 673
<212> DNA
<213> Artificial Sequence


<220> 
<223> Chimeric CMV promoter

<400> 5
tggccattgc atacgttgta tccatatcat aatatgtaca tttatattgg ctcatgtcca      60

acattaccgc catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg     120

tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg     180

cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata     240

gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc     300

cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac     360

ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg     420

cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc     480

aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc     540

aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc     600

gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct     660

cgtttagtga acc                                                        673


<210> 6
<211> 380
<212> DNA
<213> Artificial Sequence


<220> 
<223> CMV enhancer

<400> 6
gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc      60

catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca     120

acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga     180

ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc     240

aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct     300

ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat     360

tagtcatcgc tattaccatg                                                 380


<210> 7
<211> 204
<212> DNA
<213> Artificial Sequence


<220> 
<223> CMV promoter

<400> 7
gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt     60

ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac    120

tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg    180

tgggaggtct atataagcag agct                                           204


<210> 8
<211> 182
<212> DNA
<213> Artificial Sequence


<220> 
<223> RU5

<400> 8
gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca     60

ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg    120

tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc    180

ag                                                                   182


<210> 9
<211> 154
<212> DNA
<213> Artificial Sequence


<220> 
<223> PBS SL23 SL123

<400> 9
tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg     60

actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca    120

aaaattttga ctagcggagg ctagaaggag agag                                154


<210> 10
<211> 126
<212> DNA
<213> Artificial Sequence


<220> 
<223> packaging signal

<400> 10
ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga     60

ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag    120

agcgtc                                                               126


<210> 11
<211> 365
<212> DNA
<213> Artificial Sequence


<220> 
<223> SL4mgag

<400> 11
atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc      60

ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg     120

agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa     180

tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata     240

atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag     300

ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg     360

ctgat                                                                 365


<210> 12
<211> 146
<212> DNA
<213> Artificial Sequence


<220> 
<223> DenvRF1

<400> 12
tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata     60

aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc    120

agagagaaaa aagagcagtg ggaata                                         146


<210> 13
<211> 234
<212> DNA
<213> Artificial Sequence


<220> 
<223> RRE

<400> 13
aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat     60

gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt    120

gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca    180

gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcct          234


<210> 14
<211> 468
<212> DNA
<213> Artificial Sequence


<220> 
<223> DenvRF2

<400> 14
gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa      60

taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa     120

caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa     180

tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac     240

aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag     300

aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc     360

gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga     420

aggtggagag agagacagag acagatccat tcgattagtg aacggatc                  468


<210> 15
<211> 118
<212> DNA
<213> Artificial Sequence


<220> 
<223> cPPT

<400> 15
ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat     60

agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaatttt      118


<210> 16
<211> 553
<212> DNA
<213> Artificial Sequence


<220> 
<223> ET Promoter

<400> 16
cgcgagttaa taattaccag cgcgggccaa ataaataatc cgcgaggggc aggtgacgtt      60

tgcccagcgc gcgctggtaa ttattaacct cgcgaatatt gattcgaggc cgcgattgcc     120

gcaatcgcga ggggcaggtg acctttgccc agcgcgcgtt cgccccgccc cggacggtat     180

cgataagctt aggagcttgg gctgcaggtc gagggcactg ggaggatgtt gagtaagatg     240

gaaaactact gatgaccctt gcagagacag agtattagga catgtttgaa caggggccgg     300

gcgatcagca ggtagctcta gaggatcccc gtctgtctgc acatttcgta gagcgagtgt     360

tccgatactc taatctccct aggcaaggtt catatttgtg taggttactt attctccttt     420

tgttgactaa gtcaataatc agaatcagca ggtttggagt cagcttggca gggatcagca     480

gcctgggttg gaaggagggg gtataaaagc cccttcacca ggagaagccg tcacacagat     540

ccacaagctc ctg                                                        553


<210> 17
<211> 158
<212> DNA
<213> Artificial Sequence


<220> 
<223> Synthetic enhancer

<400> 17
cgcgagttaa taattaccag cgcgggccaa ataaataatc cgcgaggggc aggtgacgtt     60

tgcccagcgc gcgctggtaa ttattaacct cgcgaatatt gattcgaggc cgcgattgcc    120

gcaatcgcga ggggcaggtg acctttgccc agcgcgcg                            158


<210> 18
<211> 100
<212> DNA
<213> Artificial Sequence


<220> 
<223> mTTR enhancer

<400> 18
cactgggagg atgttgagta agatggaaaa ctactgatga cccttgcaga gacagagtat     60

taggacatgt ttgaacaggg gccgggcgat cagcaggtag                          100


<210> 19
<211> 202
<212> DNA
<213> Artificial Sequence


<220> 
<223> mTTR promoter

<400> 19
gtctgtctgc acatttcgta gagcgagtgt tccgatactc taatctccct aggcaaggtt     60

catatttgtg taggttactt attctccttt tgttgactaa gtcaataatc agaatcagca    120

ggtttggagt cagcttggca gggatcagca gcctgggttg gaaggagggg gtataaaagc    180

cccttcacca ggagaagccg tc                                             202


<210> 20
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> mTTR 5’ UT

<400> 20
acacagatcc acaagctcct g                                              21


<210> 21
<211> 501
<212> DNA
<213> Artificial Sequence


<220> 
<223> Mutated WPRE

<400> 21
caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct      60

tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg     120

gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg     180

cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt     240

tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt     300

gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg     360

ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc     420

tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat     480

ccagcggacc ttccttcccg c                                               501


<210> 22
<211> 23
<212> DNA
<213> Artificial Sequence


<220> 
<223> miRNA-142-3pT

<400> 22
tccataaagt aggaaacact aca                                            23


<210> 23
<211> 234
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3’ LTR: DR3RU5

<400> 23
tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc     60

tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta    120

agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact    180

ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agca          234


<210> 24
<211> 53
<212> DNA
<213> Artificial Sequence


<220> 
<223> ΔU3

<400> 24
tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt act            53


<210> 25
<211> 182
<212> DNA
<213> Artificial Sequence


<220> 
<223> RU5

<400> 25
gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca     60

ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg    120

tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc    180

ag                                                                   182


<210> 26
<211> 4769
<212> DNA
<213> Artificial Sequence


<220> 
<223> LV.ET.eGFP.142-3pT sequence

<400> 26
tggccattgc atacgttgta tccatatcat aatatgtaca tttatattgg ctcatgtcca       60

acattaccgc catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg      120

tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg      180

cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata      240

gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc      300

cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac      360

ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg      420

cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc      480

aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc      540

aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc      600

gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct      660

cgtttagtga accggggtct ctctggttag accagatctg agcctgggag ctctctggct      720

aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt      780

gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt      840

ggaaaatctc tagcagtggc gcccgaacag ggacctgaaa gcgaaaggga aaccagagct      900

ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact      960

ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag     1020

cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg     1080

gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt     1140

cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct     1200

acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac     1260

cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat     1320

agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac     1380

ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa     1440

aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa     1500

aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta     1560

tgggcgcagc ctcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc     1620

agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag     1680

tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc     1740

aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt     1800

ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg     1860

agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc     1920

aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt     1980

ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag     2040

gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc     2100

agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc     2160

ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga     2220

acggatctcg acggtatcgg ttaactttta aaagaaaagg ggggattggg gggtacagtg     2280

caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac     2340

aaattacaaa aattcaaaat tttatcgatc acgagactag cctcgatcga ggtcaattca     2400

cgcgagttaa taattaccag cgcgggccaa ataaataatc cgcgaggggc aggtgacgtt     2460

tgcccagcgc gcgctggtaa ttattaacct cgcgaatatt gattcgaggc cgcgattgcc     2520

gcaatcgcga ggggcaggtg acctttgccc agcgcgcgtt cgccccgccc cggacggtat     2580

cgataagctt aggagcttgg gctgcaggtc gagggcactg ggaggatgtt gagtaagatg     2640

gaaaactact gatgaccctt gcagagacag agtattagga catgtttgaa caggggccgg     2700

gcgatcagca ggtagctcta gaggatcccc gtctgtctgc acatttcgta gagcgagtgt     2760

tccgatactc taatctccct aggcaaggtt catatttgtg taggttactt attctccttt     2820

tgttgactaa gtcaataatc agaatcagca ggtttggagt cagcttggca gggatcagca     2880

gcctgggttg gaaggagggg gtataaaagc cccttcacca ggagaagccg tcacacagat     2940

ccacaagctc ctgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc     3000

atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc     3060

gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg     3120

cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc     3180

taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc     3240

caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag     3300

ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac     3360

ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg     3420

gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac     3480

ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg     3540

ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag     3600

aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg     3660

gacgagctgt acaagtaaag cggcctcgac gggcccgcgg aatttcgaca atcaacctct     3720

ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct     3780

atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat     3840

tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt     3900

caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat     3960

tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc     4020

ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga     4080

caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc     4140

cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga     4200

ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc     4260

tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattcgag ctccaccgcg     4320

gtggcggccg ctctagagtc gactccataa agtaggaaac actacacgat tccataaagt     4380

aggaaacact acaaccggtt ccataaagta ggaaacacta catcactcca taaagtagga     4440

aacactacac tcgagggggg gcccggtacc tttaagacca atgacttaca aggcagctgt     4500

agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg     4560

aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg     4620

ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt     4680

gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc     4740

cttttagtca gtgtggaaaa tctctagca                                       4769


<210> 27
<211> 29
<212> DNA
<213> Artificial Sequence


<220> 
<223> Forward

<400> 27
ctagctagca tggcctctca caagctgct                                      29


<210> 28
<211> 29
<212> DNA
<213> Artificial Sequence


<220> 
<223> Reverse

<400> 28
caagtcgact cacagcttct tcttggggc                                      29


<210> 29
<211> 1179
<212> DNA
<213> Artificial Sequence


<220> 
<223> AGXT-RHEAM

<400> 29
atggcctctc acaagctgct ggtgaccccc cccaaggccc tgctcaagcc cctctccatc      60

cccaaccgtc tcctgctggg gcctggtcct tccaacctgc ctcctcgcat catggcagcc     120

ggggggctgc agatgatcgg gcacatgagc aaggaaatgt accagatcat ggacgagatc     180

aaggaaggca tccagtacgt gttccagacc aggaacccac tcacactggt catctccggc     240

tcgggacact gtgccctgga ggccgccctg gtcaatgtgc tggagcctgg ggactccttc     300

ctggttgggg ccaatggcat ttgggggcag cgagccgcgg acatcgggga gcgcatagga     360

gcccgagtgc acccgatgac caaggacccc ggaggccact acacactgca ggaggtggag     420

gagggcctgg cccagcacaa gccagtgctg ctgttcttaa cccacgggga gtcgtccacc     480

ggcgtgctgc agccccttga tggcttcggg gaactctgcc acaggtacaa gtgcctgctc     540

ctggtggatt cggtggcatc cctgggcggg accccccttt acatggaccg gcaaggcatc     600

gacatcctgt actcgggctc ccagaaggcc ctgaacgccc ctccagggac ctcgctcatc     660

tccttcagtg acaaggccaa aaagaagatg tactcccgca agacgaagcc cttctccttc     720

tacctggaca tcaagtggct ggccaacttc tggggctgtg acgaccagcc caggatgtac     780

catcacacaa tccccgtcat cagcctgtac agcctgagag agagcctggc cctcattgcg     840

gaacagggcc tggagaacag ctggcgccag caccgcgagg ccgcggcgta tctgcatggg     900

cgcctgcagg cactggggct gcagctcttc gtgaaggacc cggcgctccg gcttcccaca     960

gtcaccactg tggctgtacc cgctggctat gactggagag acatcgtcag ctacgtcatg    1020

gaccacttcg acattgagat catgggtggc cttgggccct ccacggggaa ggtgctgcgg    1080

atcggcctgc tgggctgcaa tgccacccgc gagaatgtgg accgcgtgac ggaggccctg    1140

agggcggccc tgcagcactg ccccaagaag aagctgtga                           1179


<210> 30
<211> 5091
<212> DNA
<213> Artificial Sequence


<220> 
<223> LV.ET.AGXT-RHEAM.142-3pT

<400> 30
tggccattgc atacgttgta tccatatcat aatatgtaca tttatattgg ctcatgtcca       60

acattaccgc catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg      120

tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg      180

cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata      240

gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc      300

cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac      360

ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg      420

cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc      480

aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc      540

aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc      600

gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct      660

cgtttagtga accggggtct ctctggttag accagatctg agcctgggag ctctctggct      720

aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt      780

gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt      840

ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga      900

gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg      960

actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga     1020

gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc     1080

agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg     1140

attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca     1200

gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc     1260

aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa     1320

gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca     1380

gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag     1440

taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag     1500

aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca     1560

ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag     1620

tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca     1680

cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg     1740

atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc     1800

cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga     1860

tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat     1920

cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt     1980

tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag     2040

taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta     2100

ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca     2160

ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag     2220

tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca     2280

gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa     2340

aacaaattac aaaaattcaa aattttatcg atcacgagac tagcctcgag cacgcgagtt     2400

aataattacc agcgcgggcc aaataaataa tccgcgaggg gcaggtgacg tttgcccagc     2460

gcgcgctggt aattattaac ctcgcgaata ttgattcgag gccgcgattg ccgcaatcgc     2520

gaggggcagg tgacctttgc ccagcgcgcg ttcgccccgc cccggacggt atcgataagc     2580

ttaggagctt gggctgcagg tcgagggcac tgggaggatg ttgagtaaga tggaaaacta     2640

ctgatgaccc ttgcagagac agagtattag gacatgtttg aacaggggcc gggcgatcag     2700

caggtagctc tagaggatcc ccgtctgtct gcacatttcg tagagcgagt gttccgatac     2760

tctaatctcc ctaggcaagg ttcatatttg tgtaggttac ttattctcct tttgttgact     2820

aagtcaataa tcagaatcag caggtttgga gtcagcttgg cagggatcag cagcctgggt     2880

tggaaggagg gggtataaaa gccccttcac caggagaagc cgtcacacag atccacaagc     2940

tcctggctag ctagcatggc ctctcacaag ctgctggtga ccccccccaa ggccctgctc     3000

aagcccctct ccatccccaa ccgtctcctg ctggggcctg gtccttccaa cctgcctcct     3060

cgcatcatgg cagccggggg gctgcagatg atcgggcaca tgagcaagga aatgtaccag     3120

atcatggacg agatcaagga aggcatccag tacgtgttcc agaccaggaa cccactcaca     3180

ctggtcatct ccggctcggg acactgtgcc ctggaggccg ccctggtcaa tgtgctggag     3240

cctggggact ccttcctggt tggggccaat ggcatttggg ggcagcgagc cgcggacatc     3300

ggggagcgca taggagcccg agtgcacccg atgaccaagg accccggagg ccactacaca     3360

ctgcaggagg tggaggaggg cctggcccag cacaagccag tgctgctgtt cttaacccac     3420

ggggagtcgt ccaccggcgt gctgcagccc cttgatggct tcggggaact ctgccacagg     3480

tacaagtgcc tgctcctggt ggattcggtg gcatccctgg gcgggacccc cctttacatg     3540

gaccggcaag gcatcgacat cctgtactcg ggctcccaga aggccctgaa cgcccctcca     3600

gggacctcgc tcatctcctt cagtgacaag gccaaaaaga agatgtactc ccgcaagacg     3660

aagcccttct ccttctacct ggacatcaag tggctggcca acttctgggg ctgtgacgac     3720

cagcccagga tgtaccatca cacaatcccc gtcatcagcc tgtacagcct gagagagagc     3780

ctggccctca ttgcggaaca gggcctggag aacagctggc gccagcaccg cgaggccgcg     3840

gcgtatctgc atgggcgcct gcaggcactg gggctgcagc tcttcgtgaa ggacccggcg     3900

ctccggcttc ccacagtcac cactgtggct gtacccgctg gctatgactg gagagacatc     3960

gtcagctacg tcatggacca cttcgacatt gagatcatgg gtggccttgg gccctccacg     4020

gggaaggtgc tgcggatcgg cctgctgggc tgcaatgcca cccgcgagaa tgtggaccgc     4080

gtgacggagg ccctgagggc ggccctgcag cactgcccca agaagaagct gtgagtcgac     4140

aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct     4200

ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt     4260

atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg     4320

tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact     4380

ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct     4440

attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg     4500

ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc     4560

gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc     4620

aatccagcgg accttccttc ccgcggcctg ctgccggctc tagataatcc ataaagtagg     4680

aaacactaca cgattccata aagtaggaaa cactacaacc ggttccataa agtaggaaac     4740

actacatcac tccataaagt aggaaacact acacccggtc gagctcggta cctttaagac     4800

caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg     4860

aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct     4920

ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc     4980

ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg     5040

gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc a              5091


<210> 31
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Forward

<400> 31
caggactcgg cttgctgaag                                                20


<210> 32
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Reverse

<400> 32
tcccccgctt aatactgacg                                                20


<210> 33
<211> 19
<212> DNA
<213> Artificial Sequence


<220> 
<223> probe

<400> 33
cgcacggcaa gaggcgagg                                                 19


<210> 34
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Forward

<400> 34
gctgtcatct cttgtgggct g                                              21


<210> 35
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Reverse

<400> 35
actcatggga gctgctggtt c                                              21


<210> 36
<211> 26
<212> DNA
<213> Artificial Sequence


<220> 
<223> probe

<400> 36
cctgtcatgc ccacacaaat ctctcc                                         26


<210> 37
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Forward

<400> 37
caggactcgg cttgctgaag                                                20


<210> 38
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Reverse

<400> 38
tcccccgctt aatactgacg                                                20


<210> 39
<211> 19
<212> DNA
<213> Artificial Sequence


<220> 
<223> probe

<400> 39
cgcacggcaa gaggcgagg                                                 19


<210> 40
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> forward

<400> 40
aaaacgagca gtgacgtgag c                                              21


<210> 41
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Reverse

<400> 41
ttcagtcatg ctgctagcgc                                                20


<210> 42
<211> 25
<212> DNA
<213> Artificial Sequence


<220> 
<223> probe

<400> 42
tgcacggaag cgtctcgtct cagtc                                          25


<210> 43
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Reverse

<400> 43
gtcttgcggg agtacatctt                                                20


<210> 44
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Forward

<400> 44
caaggcatcg acatcctgta                                                20


<210> 45
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Reverse

<400> 45
gatgggaatt ccaggagtca                                                20


<210> 46
<211> 19
<212> DNA
<213> Artificial Sequence


<220> 
<223> Forward

<400> 46
gggagaatca tggaccaga                                                 19


<210> 47
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Reverse

<400> 47
ctaaggccaa ccgtgaaaag                                                20


<210> 48
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Forward

<400> 48
accagaggca tacagggaca                                                20


<210> 49
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> Reverse

<400> 49
gacaaagcca gtctccttct ac                                             22


<210> 50
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> Forward

<400> 50
gtgacaggtg tggtatgatg aa                                             22


<210> 51
<211> 720
<212> DNA
<213> Artificial Sequence


<220> 
<223> eGFP sequence

<400> 51
tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg      60

gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg     120

gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc     180

tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc     240

agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct     300

tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg     360

tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca     420

agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg     480

gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg     540

accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact     600

acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc     660

tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa     720


<210> 52
<211> 392
<212> PRT
<213> Artificial Sequence


<220> 
<223> AGXT-RHEAM. Amino acid sequence

<400> 52
Met Ala Ser His Lys Leu Leu Val Thr Pro Pro Lys Ala Leu Leu Lys 
1               5                   10                  15      
Pro Leu Ser Ile Pro Asn Arg Leu Leu Leu Gly Pro Gly Pro Ser Asn 
            20                  25                  30          
Leu Pro Pro Arg Ile Met Ala Ala Gly Gly Leu Gln Met Ile Gly His 
        35                  40                  45              
Met Ser Lys Glu Met Tyr Gln Ile Met Asp Glu Ile Lys Glu Gly Ile 
    50                  55                  60                  
Gln Tyr Val Phe Gln Thr Arg Asn Pro Leu Thr Leu Val Ile Ser Gly 
65                  70                  75                  80  
Ser Gly His Cys Ala Leu Glu Ala Ala Leu Val Asn Val Leu Glu Pro 
                85                  90                  95      
Gly Asp Ser Phe Leu Val Gly Ala Asn Gly Ile Trp Gly Gln Arg Ala 
            100                 105                 110         
Ala Asp Ile Gly Glu Arg Ile Gly Ala Arg Val His Pro Met Thr Lys 
        115                 120                 125             
Asp Pro Gly Gly His Tyr Thr Leu Gln Glu Val Glu Glu Gly Leu Ala 
    130                 135                 140                 
Gln His Lys Pro Val Leu Leu Phe Leu Thr His Gly Glu Ser Ser Thr 
145                 150                 155                 160 
Gly Val Leu Gln Pro Leu Asp Gly Phe Gly Glu Leu Cys His Arg Tyr 
                165                 170                 175     
Lys Cys Leu Leu Leu Val Asp Ser Val Ala Ser Leu Gly Gly Thr Pro 
            180                 185                 190         
Leu Tyr Met Asp Arg Gln Gly Ile Asp Ile Leu Tyr Ser Gly Ser Gln 
        195                 200                 205             
Lys Ala Leu Asn Ala Pro Pro Gly Thr Ser Leu Ile Ser Phe Ser Asp 
    210                 215                 220                 
Lys Ala Lys Lys Lys Met Tyr Ser Arg Lys Thr Lys Pro Phe Ser Phe 
225                 230                 235                 240 
Tyr Leu Asp Ile Lys Trp Leu Ala Asn Phe Trp Gly Cys Asp Asp Gln 
                245                 250                 255     
Pro Arg Met Tyr His His Thr Ile Pro Val Ile Ser Leu Tyr Ser Leu 
            260                 265                 270         
Arg Glu Ser Leu Ala Leu Ile Ala Glu Gln Gly Leu Glu Asn Ser Trp 
        275                 280                 285             
Arg Gln His Arg Glu Ala Ala Ala Tyr Leu His Gly Arg Leu Gln Ala 
    290                 295                 300                 
Leu Gly Leu Gln Leu Phe Val Lys Asp Pro Ala Leu Arg Leu Pro Thr 
305                 310                 315                 320 
Val Thr Thr Val Ala Val Pro Ala Gly Tyr Asp Trp Arg Asp Ile Val 
                325                 330                 335     
Ser Tyr Val Met Asp His Phe Asp Ile Glu Ile Met Gly Gly Leu Gly 
            340                 345                 350         
Pro Ser Thr Gly Lys Val Leu Arg Ile Gly Leu Leu Gly Cys Asn Ala 
        355                 360                 365             
Thr Arg Glu Asn Val Asp Arg Val Thr Glu Ala Leu Arg Ala Ala Leu 
    370                 375                 380                 
Gln His Cys Pro Lys Lys Lys Leu 
385                 390         

