                         SEQUENCE LISTING

<110>  ALETHIA BIOTHERAPEUTICS INC.
 
<120>  TUMOR INFILTRATING LYMPHOCYTES THERAPY

<130>  11504-OO7-PCT

<140>  
<141>  

<160>  41    

<170>  PatentIn version 3.5

<210>  1
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  16B5 CDRL1

<400>  1

Lys Ser Ser Gln Ser Leu Leu Asn Ser Arg Thr Arg Lys Asn Tyr Leu 
1               5                   10                  15      


Ala 
    


<210>  2
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  16B5 CDRL2

<400>  2

Trp Ala Ser Thr Arg Glu Ser 
1               5           


<210>  3
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  16B5 CDRL3

<400>  3

Lys Gln Ser Tyr Asn Leu Trp Thr 
1               5               


<210>  4
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  16B5 CDRH1-V1

<400>  4

Gly Phe Asn Ile Lys Asp Ile Tyr Met His 
1               5                   10  


<210>  5
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  16B5 CDRH2-V1

<400>  5

Arg Ile Asp Pro Ala Tyr Gly Asn Thr Lys Tyr Asp Pro Lys Phe Gln 
1               5                   10                  15      


Gly 
    


<210>  6
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  16B5 CDRH3-V1

<400>  6

Arg Tyr Asp Thr Ala Met Asp Tyr 
1               5               


<210>  7
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Murine 16B5 VL

<400>  7

Asp Ile Val Met Ser Gln Ser Pro Ser Ser Leu Ala Val Ser Ala Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Ser Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser 
            20                  25                  30          


Arg Thr Arg Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Ser Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Val Gln Ala Glu Asp Leu Ala Val Tyr Tyr Cys Lys Gln 
                85                  90                  95      


Ser Tyr Asn Leu Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Phe Lys 
            100                 105                 110         


<210>  8
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Murine 16B5 VH

<400>  8

Glu Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Arg Leu Ser Cys Thr Thr Ser Gly Phe Asn Ile Lys Asp Ile 
            20                  25                  30          


Tyr Met His Trp Val Lys Gln Arg Pro Glu Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Arg Ile Asp Pro Ala Tyr Gly Asn Thr Lys Tyr Asp Pro Lys Phe 
    50                  55                  60                  


Gln Gly Lys Ala Thr Ile Thr Ala Asp Thr Ser Ser Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Tyr Asp Thr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  9
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized 16B5 VL

<400>  9

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser 
            20                  25                  30          


Arg Thr Arg Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Lys Gln 
                85                  90                  95      


Ser Tyr Asn Leu Trp Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


<210>  10
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized 16B5 VH

<400>  10

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Thr Val Lys Ile Ser Cys Lys Val Ser Gly Phe Asn Ile Lys Asp Ile 
            20                  25                  30          


Tyr Met His Trp Val Gln Gln Ala Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Arg Ile Asp Pro Ala Tyr Gly Asn Thr Lys Tyr Asp Pro Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Thr Ser Thr Asp Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Tyr Asp Thr Ala Met Asp Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  11
<211>  217
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized 16B5 light chain

<400>  11

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser 
            20                  25                  30          


Arg Thr Arg Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Lys Gln 
                85                  90                  95      


Ser Tyr Asn Leu Trp Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu 
        115                 120                 125             


Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro 
    130                 135                 140                 


Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly 
145                 150                 155                 160 


Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr 
                165                 170                 175     


Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His 
            180                 185                 190         


Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val 
        195                 200                 205             


Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215         


<210>  12
<211>  443
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized 16B5 heavy chain

<400>  12

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Thr Val Lys Ile Ser Cys Lys Val Ser Gly Phe Asn Ile Lys Asp Ile 
            20                  25                  30          


Tyr Met His Trp Val Gln Gln Ala Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Arg Ile Asp Pro Ala Tyr Gly Asn Thr Lys Tyr Asp Pro Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Thr Ser Thr Asp Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Tyr Asp Thr Ala Met Asp Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu 
        115                 120                 125             


Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys 
    130                 135                 140                 


Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser 
145                 150                 155                 160 


Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser 
                165                 170                 175     


Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Asn 
            180                 185                 190         


Phe Gly Thr Gln Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn 
        195                 200                 205             


Thr Lys Val Asp Lys Thr Val Glu Arg Lys Cys Cys Val Glu Cys Pro 
    210                 215                 220                 


Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro 
225                 230                 235                 240 


Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr 
                245                 250                 255     


Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Gln Phe Asn 
            260                 265                 270         


Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg 
        275                 280                 285             


Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val Ser Val Leu Thr Val 
    290                 295                 300                 


Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser 
305                 310                 315                 320 


Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys 
                325                 330                 335     


Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu 
            340                 345                 350         


Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe 
        355                 360                 365             


Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 
    370                 375                 380                 


Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp Ser Asp Gly Ser Phe 
385                 390                 395                 400 


Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly 
                405                 410                 415     


Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr 
            420                 425                 430         


Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        435                 440             


<210>  13
<211>  720
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Humanized 16B5 light chain nucleic acid

<400>  13
atggtgctgc agacccaggt gttcatctcc ctgctgctgt ggatctccgg cgcctacggc       60

gacatcgtga tgacccagtc ccccgactcc ctggccgtgt ccctgggcga gagagccacc      120

atcaactgca agtcctccca gtccctgctg aactcccgga cccggaagaa ctacctggcc      180

tggtatcagc agaagcctgg ccagcctcct aagctgctga tctactgggc ctccacccgg      240

gagtccggcg tgcctgaccg gttctccggc tccggcagcg gcaccgactt caccctgacc      300

atcagctccc tgcaggccga ggacgtggcc gtgtactact gcaagcagtc ctacaacctg      360

tggaccttcg gccagggcac caagctggag atcaagcgga ctgtggctgc accatctgtc      420

ttcatcttcc cgccatctga tgagcagttg aaatctggaa ctgcctctgt tgtgtgcctg      480

ctgaataact tctatcccag agaggccaaa gtacagtgga aggtggataa cgccctccaa      540

tcgggtaact cccaggagag tgtcacagag caggacagca aggacagcac ctacagcctc      600

agcagcaccc tgacgctgag caaagcagac tacgagaaac acaaagtcta cgcctgcgaa      660

gtcacccatc agggcctgag ctcgcccgtc acaaagagct tcaacagggg agagtgttag      720


<210>  14
<211>  1389
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Humanized 16B5 heavy chain nucleic acid

<400>  14
atggactgga cctggcggat cctgttcctg gtggccgctg ctaccggcac ccacgcccag       60

gtgcagctgg tgcagtctgg cgccgaggtg aagaagcctg gcgccaccgt caagatcagc      120

tgcaaggtgt ccggcttcaa catcaaggac atctacatgc actgggtgca gcaggctcca      180

ggcaagggac tggagtggat gggccggatc gaccctgcct acggcaacac caagtacgac      240

cctaagttcc agggccgggt gaccatcacc gccgacacct ccaccgacac cgcctacatg      300

gaactgtcct ccctgcggtc cgaggacacc gccgtgtact actgcgcccg gagatacgac      360

accgccatgg attactgggg ccagggcacc ctggtgaccg tgtcctccgc ttccaccaag      420

ggcccatcgg tcttccccct ggcgccctgc tccaggagca cctccgagag cacagcggcc      480

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc      540

gctctgacca gcggcgtgca caccttccca gctgtcctac agtcctcagg actctactcc      600

ctcagcagcg tggtgaccgt gccctccagc aacttcggca cccagaccta cacctgcaac      660

gtagatcaca agcccagcaa caccaaggtg gacaagacag ttgagcgcaa atgttgtgtc      720

gagtgcccac cgtgcccagc accacctgtg gcaggaccgt cagtcttcct cttcccccca      780

aaacccaagg acaccctcat gatctcccgg acccctgagg tcacgtgcgt ggtggtggac      840

gtgagccacg aagaccccga ggtccagttc aactggtacg tggacggcgt ggaggtgcat      900

aatgccaaga caaagccacg ggaggagcag ttcaacagca cgttccgtgt ggtcagcgtc      960

ctcaccgttg tgcaccagga ctggctgaac ggcaaggagt acaagtgcaa ggtctccaac     1020

aaaggcctcc cagcccccat cgagaaaacc atctccaaaa ccaaagggca gccccgagaa     1080

ccacaggtgt acaccctgcc cccatcccgg gaggagatga ccaagaacca ggtcagcctg     1140

acctgcctgg tcaaaggctt ctaccccagc gacatcgccg tggagtggga gagcaatggg     1200

cagccggaga acaactacaa gaccacacct cccatgctgg actccgacgg ctccttcttc     1260

ctctacagca agctcaccgt ggacaagagc aggtggcagc aggggaacgt cttctcatgc     1320

tccgtgatgc atgaggctct gcacaaccac tacacgcaga agagcctctc cctgtctccg     1380

ggtaaatga                                                             1389


<210>  15
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  21B12 CDRL1

<400>  15

Lys Ser Ser Gln Ser Leu Leu Tyr Ser Ser Asn Gln Lys Asn Tyr Leu 
1               5                   10                  15      


Ala 
    


<210>  16
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  21B12 CDRL2

<400>  16

Trp Ala Ser Thr Arg Glu Ser 
1               5           


<210>  17
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  21B12 CDRL3

<400>  17

Gln Gln Tyr Tyr Ile Tyr Pro Arg Thr 
1               5                   


<210>  18
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  21B12 CDRH1-v1

<400>  18

Gly Tyr Thr Phe Thr Asn Tyr Gly Met His 
1               5                   10  


<210>  19
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  21B12 CDRH2-v1

<400>  19

Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr Ala Asp Asp Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  20
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  21B12 CDRH3-v1

<400>  20

Asp Gly Phe Leu Tyr Phe Phe Asp Tyr 
1               5                   


<210>  21
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Murine 21B12 VL

<400>  21

Asp Ile Val Met Ser Gln Ser Pro Ser Ser Leu Ala Val Ser Val Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Ser Cys Lys Ser Ser Gln Ser Leu Leu Tyr Ser 
            20                  25                  30          


Ser Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Arg Pro Gly Gln 
        35                  40                  45              


Ser Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Val Lys Ala Glu Asp Leu Ala Val Tyr Tyr Cys Gln Gln 
                85                  90                  95      


Tyr Tyr Ile Tyr Pro Arg Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
            100                 105                 110         


Lys 
    


<210>  22
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Murine 21B12 VH

<400>  22

Gln Ile Gln Leu Val Gln Ser Gly Pro Glu Leu Lys Lys Pro Gly Glu 
1               5                   10                  15      


Thr Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Gly Met His Trp Val Lys Gln Ala Pro Gly Lys Gly Leu Lys Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr Ala Asp Asp Phe 
    50                  55                  60                  


Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Ala Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Ile Asn Asn Leu Lys Asn Glu Asp Thr Ala Thr Tyr Phe Cys 
                85                  90                  95      


Ala Arg Asp Gly Phe Leu Tyr Phe Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Thr Leu Thr Val Ser Ser 
        115             


<210>  23
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized 21B12 VL

<400>  23

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Leu Leu Tyr Ser 
            20                  25                  30          


Ser Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln 
                85                  90                  95      


Tyr Tyr Ile Tyr Pro Arg Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile 
            100                 105                 110         


Lys 
    


<210>  24
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized 21B12 VH

<400>  24

Gln Val Gln Leu Val Gln Ser Gly Ser Glu Leu Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr Ala Asp Asp Phe 
    50                  55                  60                  


Lys Gly Arg Phe Val Phe Ser Leu Asp Thr Ser Val Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Ile Ser Ser Leu Lys Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Asp Gly Phe Leu Tyr Phe Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  25
<211>  220
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized 21B12 light chain

<400>  25

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Leu Leu Tyr Ser 
            20                  25                  30          


Ser Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln 
                85                  90                  95      


Tyr Tyr Ile Tyr Pro Arg Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile 
            100                 105                 110         


Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp 
        115                 120                 125             


Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn 
    130                 135                 140                 


Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu 
145                 150                 155                 160 


Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp 
                165                 170                 175     


Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr 
            180                 185                 190         


Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser 
        195                 200                 205             


Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215                 220 


<210>  26
<211>  444
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized 21B12 heavy chain

<400>  26

Gln Val Gln Leu Val Gln Ser Gly Ser Glu Leu Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr Ala Asp Asp Phe 
    50                  55                  60                  


Lys Gly Arg Phe Val Phe Ser Leu Asp Thr Ser Val Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Ile Ser Ser Leu Lys Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Asp Gly Phe Leu Tyr Phe Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Asn Phe Gly Thr Gln Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Thr Val Glu Arg Lys Cys Cys Val Glu Cys 
    210                 215                 220                 


Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe 
225                 230                 235                 240 


Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val 
                245                 250                 255     


Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Gln Phe 
            260                 265                 270         


Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro 
        275                 280                 285             


Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val Ser Val Leu Thr 
    290                 295                 300                 


Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val 
305                 310                 315                 320 


Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr 
                325                 330                 335     


Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg 
            340                 345                 350         


Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly 
        355                 360                 365             


Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro 
    370                 375                 380                 


Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp Ser Asp Gly Ser 
385                 390                 395                 400 


Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln 
                405                 410                 415     


Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His 
            420                 425                 430         


Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        435                 440                 


<210>  27
<211>  723
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Humanized 21B12 light chain nucleic acid

<400>  27
atggtgctgc agacccaggt gttcatctcc ctgctgctgt ggatctctgg cgcctacggc       60

gacatcgtga tgacccagtc ccccgactct ctggctgtgt ccctgggcga gcgggccacc      120

atcaactgca agtcctccca gtccctgctg tactcctcca accagaagaa ctacctggcc      180

tggtatcagc agaagcctgg ccagcctcct aagctgctga tctactgggc ctccacccgg      240

gaatctggcg tgcctgaccg gttctccggc tctggctccg gcaccgactt caccctgacc      300

atcagctccc tgcaggccga ggacgtggcc gtgtactact gccagcagta ctacatctac      360

cctcggacct tcggccaggg caccaagctg gaaatcaagc ggaccgtggc cgctccttcc      420

gtgttcatct tccccccttc cgacgagcag ctgaagtccg gcaccgcctc tgtggtgtgc      480

ctgctgaaca acttctaccc ccgggaggcc aaggtgcagt ggaaggtgga caacgccctg      540

cagtccggca actcccagga atccgtcacc gagcaggact ccaaggactc tacctactcc      600

ctgtcctcca ccctgaccct gtccaaggcc gactacgaga agcacaaggt gtacgcctgc      660

gaagtgaccc accagggcct gtcctctccc gtgaccaagt ccttcaaccg gggcgagtgc      720

tga                                                                    723


<210>  28
<211>  1392
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Humanized 21B12 heavy chain nucleic acid

<400>  28
atggactgga cctggcggat cctgtttctg gtggccgctg ctaccggcac acacgcccag       60

gtgcagctgg tgcagtccgg ctccgagctg aagaaacctg gcgcctccgt gaaggtgtcc      120

tgcaaggcct ccggctacac cttcaccaac tacggcatgc actgggtgcg ccaggcacct      180

ggacagggac tggaatggat gggctggatc aacacctaca ccggcgagcc tacctacgcc      240

gacgacttca agggcagatt cgtgttctcc ctggacacct ccgtgtccac cgcctacctg      300

cagatctcct ccctgaaggc cgaggacacc gccgtgtact actgcgccag ggacggcttc      360

ctgtacttct tcgactactg gggccagggc accctggtga ccgtgtcctc tgcctccacc      420

aagggccctt ccgtgttccc tctggcccct tgctcccggt ccacctctga gtctaccgcc      480

gctctgggct gcctggtgaa ggactacttc cctgagcctg tgacagtgtc ctggaactct      540

ggcgccctga cctctggcgt gcacaccttc cctgccgtgc tgcagtcctc cggcctgtac      600

tccctgtcct ccgtggtgac agtgccttcc tccaacttcg gcacccagac ctacacctgc      660

aacgtggacc acaagccttc caacaccaag gtggacaaga ccgtggagcg gaagtgctgc      720

gtggagtgcc ctccttgtcc tgctcctcct gtggctggcc ctagcgtgtt cctgttccct      780

cctaagccta aggacaccct gatgatctcc cggacccctg aagtgacctg cgtggtggtg      840

gacgtgtccc acgaggaccc tgaggtgcag ttcaattggt acgtggacgg cgtggaggtg      900

cacaacgcca agaccaagcc tcgggaggaa cagttcaact ccaccttccg ggtggtgtcc      960

gtgctgaccg tggtgcacca ggactggctg aacggcaaag aatacaagtg caaggtgtcc     1020

aacaagggcc tgcctgcccc tatcgaaaag accatctcta agaccaaggg ccagcctcgc     1080

gagcctcagg tgtacaccct gcctccctcc cgcgaggaaa tgaccaagaa ccaggtgtcc     1140

ctgacctgtc tggtgaaggg cttctaccct tccgatatcg ccgtggagtg ggagtctaac     1200

ggccagcctg agaacaacta caagaccacc cctcctatgc tggactccga cggctccttc     1260

ttcctgtaca gcaagctgac agtggacaag tcccggtggc agcagggcaa cgtgttctcc     1320

tgctccgtga tgcacgaggc cctgcacaac cactacaccc agaagtccct gtccctgtct     1380

cctggcaagt ga                                                         1392


<210>  29
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  20E11 variable light chain

<400>  29

Asp Ile Val Leu Thr Leu Ser Pro Ala Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Gln Ser Val Asn Ser Ser 
            20                  25                  30          


Asn Tyr Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Lys Tyr Ala Ser Asn Leu Glu Ser Gly Val Pro Ala 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr His Phe Thr Leu Asn Ile His 
65                  70                  75                  80  


Pro Val Glu Glu Glu Asp Thr Ala Thr Tyr Tyr Cys Gln His Ser Trp 
                85                  90                  95      


Glu Ile Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110     


<210>  30
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  20E11 variable heavy chain

<400>  30

Gln Ile Gln Leu Val Gln Ser Gly Pro Glu Leu Lys Lys Pro Gly Glu 
1               5                   10                  15      


Thr Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Ser Met His Trp Val Lys Gln Ala Pro Gly Lys Gly Leu Lys Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Thr Glu Thr Gly Glu Pro Thr Tyr Ala Asp Asp Phe 
    50                  55                  60                  


Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Ala Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Ile Asn Asn Leu Lys Asn Glu Asp Thr Ala Thr Tyr Phe Cys 
                85                  90                  95      


Ala Arg Thr Gly Ser Ser Gly Tyr Phe Asp Cys Trp Gly Gln Gly Thr 
            100                 105                 110         


Thr Leu Thr Val Ser Ser 
        115             


<210>  31
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  11E2 variable light chain Gr1

<400>  31

Glu Asn Val Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 
            20                  25                  30          


His Trp Tyr Gln Gln Lys Ser Ser Thr Ser Pro Lys Leu Trp Ile Tyr 
        35                  40                  45              


Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Gly Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Asn Ser Tyr Ser Leu Thr Ile Ser Ser Met Glu Ala Glu 
65                  70                  75                  80  


Asp Val Ala Thr Tyr Tyr Cys Phe Gln Gly Ser Gly Tyr Pro Phe Thr 
                85                  90                  95      


Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys 
            100                 105     


<210>  32
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  11E2 variable light chain Gr2

<400>  32

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Gly Lys Val Thr Ile Thr Cys Lys Ala Ser Gln Asp Ile Asn Lys Tyr 
            20                  25                  30          


Ile Ala Trp Tyr Gln His Lys Pro Gly Lys Gly Pro Arg Leu Leu Ile 
        35                  40                  45              


His Tyr Thr Ser Thr Leu Gln Pro Gly Ile Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Arg Asp Tyr Ser Phe Ser Ile Ser Asn Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Tyr Cys Leu Gln Tyr Asp Asn Leu Leu Arg 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  33
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  11E2 variable heavy chain

<400>  33

Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Glu Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Gly Tyr 
            20                  25                  30          


Asn Met Asn Trp Val Lys Gln Asn Asn Gly Lys Ser Leu Glu Trp Ile 
        35                  40                  45              


Gly Asn Ile Asp Pro Tyr Tyr Gly Thr Pro Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Lys Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Leu Asn Ser Leu Leu Arg Leu Asn Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Ser Val Thr Val Ser Ser 
        115                 120 


<210>  34
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  16C11 variable heavy chain

<400>  34

Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Gly Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Gly Tyr 
            20                  25                  30          


Asn Met Tyr Trp Val Lys Gln Ser His Arg Lys Ser Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asp Pro Tyr Asn Gly Asp Thr Ser Tyr Asn Gln Lys Ser 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Arg Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met His Leu Asn Ser Leu Thr Ser Glu Asp Ser Gly Ile Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Ala Tyr Gly Ser Ser Tyr Ala Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Ala Val Ser Ala 
        115             


<210>  35
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  16B5 CDRH1-v2

<400>  35

Gly Phe Asn Ile Lys Asp Ile Tyr 
1               5               


<210>  36
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  16B5 CDRH2-v2

<400>  36

Ile Asp Pro Ala Tyr Gly Asn Thr 
1               5               


<210>  37
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  16B5 CDRH3-v2

<400>  37

Ala Arg Arg Tyr Asp Thr Ala Met Asp Tyr 
1               5                   10  


<210>  38
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  21B12 CDRH1-v2

<400>  38

Gly Tyr Thr Phe Thr Asn Tyr Gly 
1               5               


<210>  39
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  21B12 CDRH2-v2

<400>  39

Ile Asn Thr Tyr Thr Gly Glu Pro 
1               5               


<210>  40
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  21B12 CDRH3-v2

<400>  40

Asp Gly Phe Leu Tyr Phe Phe Asp Tyr 
1               5                   


<210>  41
<211>  75
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acids 421-443 of C-terminal portion of beta subunit of 
       human clusterin

<400>  41

Leu Thr Gln Gly Glu Asp Gln Tyr Tyr Leu Arg Val Thr Thr Val Ala 
1               5                   10                  15      


Ser His Thr Ser Asp Ser Asp Val Pro Ser Gly Val Thr Glu Val Val 
            20                  25                  30          


Val Lys Leu Phe Asp Ser Asp Pro Ile Thr Val Thr Val Pro Val Glu 
        35                  40                  45              


Val Ser Arg Lys Asn Pro Lys Phe Met Glu Thr Val Ala Glu Lys Ala 
    50                  55                  60                  


Leu Gln Glu Tyr Arg Lys Lys His Arg Glu Glu 
65                  70                  75  


