                         SEQUENCE LISTING

<110>  Northwestern University
       Fang, Deyu
       Montauti, Elena
       Yan, Ming
       Gao, Biexue
       Tang, Amy
       Liu, Huiping
 
<120>  INHIBITORS OF UBIQUITIN SPECIFIC PEPTIDASE 22 (USP22) AND USES 
       THEREOF FOR TREATING DISEASES AND DISORDERS

<130>  702581.02132

<150>  63/201,330
<151>  2021-04-23

<160>  50    

<170>  PatentIn version 3.5

<210>  1
<211>  525
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Val Ser Arg Pro Glu Pro Glu Gly Glu Ala Met Asp Ala Glu Leu 
1               5                   10                  15      


Ala Val Ala Pro Pro Gly Cys Ser His Leu Gly Ser Phe Lys Val Asp 
            20                  25                  30          


Asn Trp Lys Gln Asn Leu Arg Ala Ile Tyr Gln Cys Phe Val Trp Ser 
        35                  40                  45              


Gly Thr Ala Glu Ala Arg Lys Arg Lys Ala Lys Ser Cys Ile Cys His 
    50                  55                  60                  


Val Cys Gly Val His Leu Asn Arg Leu His Ser Cys Leu Tyr Cys Val 
65                  70                  75                  80  


Phe Phe Gly Cys Phe Thr Lys Lys His Ile His Glu His Ala Lys Ala 
                85                  90                  95      


Lys Arg His Asn Leu Ala Ile Asp Leu Met Tyr Gly Gly Ile Tyr Cys 
            100                 105                 110         


Phe Leu Cys Gln Asp Tyr Ile Tyr Asp Lys Asp Met Glu Ile Ile Ala 
        115                 120                 125             


Lys Glu Glu Gln Arg Lys Ala Trp Lys Met Gln Gly Val Gly Glu Lys 
    130                 135                 140                 


Phe Ser Thr Trp Glu Pro Thr Lys Arg Glu Leu Glu Leu Leu Lys His 
145                 150                 155                 160 


Asn Pro Lys Arg Arg Lys Ile Thr Ser Asn Cys Thr Ile Gly Leu Arg 
                165                 170                 175     


Gly Leu Ile Asn Leu Gly Asn Thr Cys Phe Met Asn Cys Ile Val Gln 
            180                 185                 190         


Ala Leu Thr His Thr Pro Leu Leu Arg Asp Phe Phe Leu Ser Asp Arg 
        195                 200                 205             


His Arg Cys Glu Met Gln Ser Pro Ser Ser Cys Leu Val Cys Glu Met 
    210                 215                 220                 


Ser Ser Leu Phe Gln Glu Phe Tyr Ser Gly His Arg Ser Pro His Ile 
225                 230                 235                 240 


Pro Tyr Lys Leu Leu His Leu Val Trp Thr His Ala Arg His Leu Ala 
                245                 250                 255     


Gly Tyr Glu Gln Gln Asp Ala His Glu Phe Leu Ile Ala Ala Leu Asp 
            260                 265                 270         


Val Leu His Arg His Cys Lys Gly Asp Asp Asn Gly Lys Lys Ala Asn 
        275                 280                 285             


Asn Pro Asn His Cys Asn Cys Ile Ile Asp Gln Ile Phe Thr Gly Gly 
    290                 295                 300                 


Leu Gln Ser Asp Val Thr Cys Gln Val Cys His Gly Val Ser Thr Thr 
305                 310                 315                 320 


Ile Asp Pro Phe Trp Asp Ile Ser Leu Asp Leu Pro Gly Ser Ser Thr 
                325                 330                 335     


Pro Phe Trp Pro Leu Ser Pro Gly Ser Glu Gly Asn Val Val Asn Gly 
            340                 345                 350         


Glu Ser His Val Ser Gly Thr Thr Thr Leu Thr Asp Cys Leu Arg Arg 
        355                 360                 365             


Phe Thr Arg Pro Glu His Leu Gly Ser Ser Ala Lys Ile Lys Cys Ser 
    370                 375                 380                 


Gly Cys His Ser Tyr Gln Glu Ser Thr Lys Gln Leu Thr Met Lys Lys 
385                 390                 395                 400 


Leu Pro Ile Val Ala Cys Phe His Leu Lys Arg Phe Glu His Ser Ala 
                405                 410                 415     


Lys Leu Arg Arg Lys Ile Thr Thr Tyr Val Ser Phe Pro Leu Glu Leu 
            420                 425                 430         


Asp Met Thr Pro Phe Met Ala Ser Ser Lys Glu Ser Arg Met Asn Gly 
        435                 440                 445             


Gln Tyr Gln Gln Pro Thr Asp Ser Leu Asn Asn Asp Asn Lys Tyr Ser 
    450                 455                 460                 


Leu Phe Ala Val Val Asn His Gln Gly Thr Leu Glu Ser Gly His Tyr 
465                 470                 475                 480 


Thr Ser Phe Ile Arg Gln His Lys Asp Gln Trp Phe Lys Cys Asp Asp 
                485                 490                 495     


Ala Ile Ile Thr Lys Ala Ser Ile Lys Asp Val Leu Asp Ser Glu Gly 
            500                 505                 510         


Tyr Leu Leu Phe Tyr His Lys Gln Phe Leu Glu Tyr Glu 
        515                 520                 525 


<210>  2
<211>  513
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Ala Pro Gly Trp Pro Ser Leu Ser Ala Gly Ser Arg Gln Glu Ala 
1               5                   10                  15      


Pro Gln Leu Ala Ala Gly Gly Ser Ala Tyr Gln Ala Val Gly Arg Gln 
            20                  25                  30          


Phe Gln Pro Arg Ala Thr Ala Leu Gln Gly Pro Ser Gln Ala Lys Ser 
        35                  40                  45              


Cys Ile Cys His Val Cys Gly Val His Leu Asn Arg Leu His Ser Cys 
    50                  55                  60                  


Leu Tyr Cys Val Phe Phe Gly Cys Phe Thr Lys Lys His Ile His Glu 
65                  70                  75                  80  


His Ala Lys Ala Lys Arg His Asn Leu Ala Ile Asp Leu Met Tyr Gly 
                85                  90                  95      


Gly Ile Tyr Cys Phe Leu Cys Gln Asp Tyr Ile Tyr Asp Lys Asp Met 
            100                 105                 110         


Glu Ile Ile Ala Lys Glu Glu Gln Arg Lys Ala Trp Lys Met Gln Gly 
        115                 120                 125             


Val Gly Glu Lys Phe Ser Thr Trp Glu Pro Thr Lys Arg Glu Leu Glu 
    130                 135                 140                 


Leu Leu Lys His Asn Pro Lys Arg Arg Lys Ile Thr Ser Asn Cys Thr 
145                 150                 155                 160 


Ile Gly Leu Arg Gly Leu Ile Asn Leu Gly Asn Thr Cys Phe Met Asn 
                165                 170                 175     


Cys Ile Val Gln Ala Leu Thr His Thr Pro Leu Leu Arg Asp Phe Phe 
            180                 185                 190         


Leu Ser Asp Arg His Arg Cys Glu Met Gln Ser Pro Ser Ser Cys Leu 
        195                 200                 205             


Val Cys Glu Met Ser Ser Leu Phe Gln Glu Phe Tyr Ser Gly His Arg 
    210                 215                 220                 


Ser Pro His Ile Pro Tyr Lys Leu Leu His Leu Val Trp Thr His Ala 
225                 230                 235                 240 


Arg His Leu Ala Gly Tyr Glu Gln Gln Asp Ala His Glu Phe Leu Ile 
                245                 250                 255     


Ala Ala Leu Asp Val Leu His Arg His Cys Lys Gly Asp Asp Asn Gly 
            260                 265                 270         


Lys Lys Ala Asn Asn Pro Asn His Cys Asn Cys Ile Ile Asp Gln Ile 
        275                 280                 285             


Phe Thr Gly Gly Leu Gln Ser Asp Val Thr Cys Gln Val Cys His Gly 
    290                 295                 300                 


Val Ser Thr Thr Ile Asp Pro Phe Trp Asp Ile Ser Leu Asp Leu Pro 
305                 310                 315                 320 


Gly Ser Ser Thr Pro Phe Trp Pro Leu Ser Pro Gly Ser Glu Gly Asn 
                325                 330                 335     


Val Val Asn Gly Glu Ser His Val Ser Gly Thr Thr Thr Leu Thr Asp 
            340                 345                 350         


Cys Leu Arg Arg Phe Thr Arg Pro Glu His Leu Gly Ser Ser Ala Lys 
        355                 360                 365             


Ile Lys Cys Ser Gly Cys His Ser Tyr Gln Glu Ser Thr Lys Gln Leu 
    370                 375                 380                 


Thr Met Lys Lys Leu Pro Ile Val Ala Cys Phe His Leu Lys Arg Phe 
385                 390                 395                 400 


Glu His Ser Ala Lys Leu Arg Arg Lys Ile Thr Thr Tyr Val Ser Phe 
                405                 410                 415     


Pro Leu Glu Leu Asp Met Thr Pro Phe Met Ala Ser Ser Lys Glu Ser 
            420                 425                 430         


Arg Met Asn Gly Gln Tyr Gln Gln Pro Thr Asp Ser Leu Asn Asn Asp 
        435                 440                 445             


Asn Lys Tyr Ser Leu Phe Ala Val Val Asn His Gln Gly Thr Leu Glu 
    450                 455                 460                 


Ser Gly His Tyr Thr Ser Phe Ile Arg Gln His Lys Asp Gln Trp Phe 
465                 470                 475                 480 


Lys Cys Asp Asp Ala Ile Ile Thr Lys Ala Ser Ile Lys Asp Val Leu 
                485                 490                 495     


Asp Ser Glu Gly Tyr Leu Leu Phe Tyr His Lys Gln Phe Leu Glu Tyr 
            500                 505                 510         


Glu 
    


<210>  3
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP22Chp1F

<400>  3
tgtattcttg ccacgcccaa                                                   20


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP22Chp1R

<400>  4
tcctagtgtg ggcgtttctg                                                   20


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP22Chp2F

<400>  5
attgcggtac ccaacacagt                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP22Chp2R

<400>  6
gcgtctgcga gttctctgaa                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP22Chp3F

<400>  7
ttcagagaac tcgcagacgc                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP22Chp3R

<400>  8
gcgtgctgag gattgggtaa                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP22Chp4F

<400>  9
ttacccaatc ctcagcacgc                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP22Chp4R

<400>  10
attggtggtt tgccggtcta                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP22Chp5F

<400>  11
cttagaccgg caaaccacca                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP22Chp5R

<400>  12
ggctccagag aaaagccgaa                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP22Chp6F

<400>  13
tcttagaccg gcaaaccacc                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP22Chp6R

<400>  14
tgtccgcggg aaaggataac                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP22Chp7F

<400>  15
tcccacctgt gttggattgc                                                   20


<210>  16
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP22Chp7R

<400>  16
gggcttccca agacaatgac t                                                 21


<210>  17
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP22Chp8F

<400>  17
aagccaaggg cttcccaag                                                    19


<210>  18
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP22Chp8R

<400>  18
actcagggca tattgtgagg g                                                 21


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP22Chp9F

<400>  19
tgtcggcaat ttttctcggc                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP22Chp9R

<400>  20
cccatgatgt ggagcagtga                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP21Pro1F

<400>  21
tgcatcggct aggaatggtc                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP21Pro1R

<400>  22
accaatcagg tcaccaagcc                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP21Pro2F

<400>  23
aggcttggtg acctgattgg                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP21Pro2R

<400>  24
gcttgttccg cagattccac                                                   20


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP21Pro3F

<400>  25
agctctcctc tgtcaagcct                                                   20


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP21Pro3R

<400>  26
aacgtagagc agcctcttgg                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP21Pro4F

<400>  27
agtggaagtc cccgatctga                                                   20


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP21Pro4R

<400>  28
ggcgtagtcc ttcattggct                                                   20


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP21Pro5F

<400>  29
agccaatgaa ggactacgcc                                                   20


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP21Pro5R

<400>  30
cctccagggc tctacttgga                                                   20


<210>  31
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP21Pro6F

<400>  31
cctggtagcc tgtggttctc                                                   20


<210>  32
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP21Pro6R

<400>  32
ctccgcgttt tgcttgttca                                                   20


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP21Pro7F

<400>  33
ggatctcccc acccttaggt                                                   20


<210>  34
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP21Pro7R

<400>  34
ggaagcaaga gggatgcagt                                                   20


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- Usp7F

<400>  35
aagtctcaag gttataggga                                                   20


<210>  36
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- Usp7R

<400>  36
ccatgcttgt ctgggtatag tgt                                               23


<210>  37
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP21F

<400>  37
tgcatgaaga acctgagttg a                                                 21


<210>  38
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP21R

<400>  38
acaggtccac aatcttgctg t                                                 21


<210>  39
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP22ex2 1F

<400>  39
gcttcaaggt ggacaactgg                                                   20


<210>  40
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- USP22ex2 1R

<400>  40
acatggcaga cacaggactt                                                   20


<210>  41
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- hUSP22F 1

<400>  41
ggaaaatgca aggcgttgga g                                                 21


<210>  42
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- hUSP22R_ 1

<400>  42
gtgcagttgg aggtgatctt t                                                 21


<210>  43
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- hUSP22F 2

<400>  43
ctttccccgt ttaccacgtt g                                                 21


<210>  44
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- hUSP22R_ 2

<400>  44
ctttccccgt ttaccacgtt g                                                 21


<210>  45
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- hUSP21F 1

<400>  45
gccacccact ttgagacgta g                                                 21


<210>  46
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- hUSP21R 1

<400>  46
tccgtatgct gaacagggta g                                                 21


<210>  47
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- hUSP7F

<400>  47
ccctccgtgt tttgtgcga                                                    19


<210>  48
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- hUSP7R

<400>  48
agaccatgac gtggaatcag a                                                 21


<210>  49
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- h18S F

<400>  49
gaggatgagg tggaacgtgt                                                   20


<210>  50
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- h18S R

<400>  50
agaagtgacg cagccctcta                                                   20


