                         SEQUENCE LISTING

<110>  Moraes, Carlos
       Smith, James J.
       Jantz, Derek
       Tomberlin, Ginger
       Morris, John
       Shoop, Wendy
 
<120>  ENGINEERED MEGANUCLEASES THAT TARGET HUMAN MITOCHONDRIAL GENOMES

<130>  P89339 1150WO (0137.9)

<150>  US 63/178,269
<151>  2021-04-22

<160>  34    

<170>  PatentIn version 3.5

<210>  1
<211>  163
<212>  PRT
<213>  Chlamydomonas reinhardtii

<400>  1

Met Asn Thr Lys Tyr Asn Lys Glu Phe Leu Leu Tyr Leu Ala Gly Phe 
1               5                   10                  15      


Val Asp Gly Asp Gly Ser Ile Ile Ala Gln Ile Lys Pro Asn Gln Ser 
            20                  25                  30          


Tyr Lys Phe Lys His Gln Leu Ser Leu Ala Phe Gln Val Thr Gln Lys 
        35                  40                  45              


Thr Gln Arg Arg Trp Phe Leu Asp Lys Leu Val Asp Glu Ile Gly Val 
    50                  55                  60                  


Gly Tyr Val Arg Asp Arg Gly Ser Val Ser Asp Tyr Ile Leu Ser Glu 
65                  70                  75                  80  


Ile Lys Pro Leu His Asn Phe Leu Thr Gln Leu Gln Pro Phe Leu Lys 
                85                  90                  95      


Leu Lys Gln Lys Gln Ala Asn Leu Val Leu Lys Ile Ile Trp Arg Leu 
            100                 105                 110         


Pro Ser Ala Lys Glu Ser Pro Asp Lys Phe Leu Glu Val Cys Thr Trp 
        115                 120                 125             


Val Asp Gln Ile Ala Ala Leu Asn Asp Ser Lys Thr Arg Lys Thr Thr 
    130                 135                 140                 


Ser Glu Thr Val Arg Ala Val Leu Asp Ser Leu Ser Glu Lys Lys Lys 
145                 150                 155                 160 


Ser Ser Pro 
            


<210>  2
<211>  354
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  2

Met Asn Thr Lys Tyr Asn Lys Glu Phe Leu Leu Tyr Leu Ala Gly Phe 
1               5                   10                  15      


Val Asp Gly Asp Gly Ser Ile Ile Ala Gln Ile Lys Pro Asn Gln Ser 
            20                  25                  30          


Tyr Lys Phe Lys His Gln Leu Ser Leu Ala Phe Gln Val Thr Gln Lys 
        35                  40                  45              


Thr Gln Arg Arg Trp Phe Leu Asp Lys Leu Val Asp Glu Ile Gly Val 
    50                  55                  60                  


Gly Tyr Val Arg Asp Arg Gly Ser Val Ser Asp Tyr Ile Leu Ser Glu 
65                  70                  75                  80  


Ile Lys Pro Leu His Asn Phe Leu Thr Gln Leu Gln Pro Phe Leu Lys 
                85                  90                  95      


Leu Lys Gln Lys Gln Ala Asn Leu Val Leu Lys Ile Ile Glu Gln Leu 
            100                 105                 110         


Pro Ser Ala Lys Glu Ser Pro Asp Lys Phe Leu Glu Val Cys Thr Trp 
        115                 120                 125             


Val Asp Gln Ile Ala Ala Leu Asn Asp Ser Lys Thr Arg Lys Thr Thr 
    130                 135                 140                 


Ser Glu Thr Val Arg Ala Val Leu Asp Ser Leu Pro Gly Ser Val Gly 
145                 150                 155                 160 


Gly Leu Ser Pro Ser Gln Ala Ser Ser Ala Ala Ser Ser Ala Ser Ser 
                165                 170                 175     


Ser Pro Gly Ser Gly Ile Ser Glu Ala Leu Arg Ala Gly Ala Gly Ser 
            180                 185                 190         


Gly Thr Gly Tyr Asn Lys Glu Phe Leu Leu Tyr Leu Ala Gly Phe Val 
        195                 200                 205             


Asp Gly Asp Gly Ser Ile Ile Ala Gln Ile Lys Pro Asn Gln Ser Tyr 
    210                 215                 220                 


Lys Phe Lys His Gln Leu Ser Leu Ala Phe Gln Val Thr Gln Lys Thr 
225                 230                 235                 240 


Gln Arg Arg Trp Phe Leu Asp Lys Leu Val Asp Glu Ile Gly Val Gly 
                245                 250                 255     


Tyr Val Arg Asp Arg Gly Ser Val Ser Asp Tyr Ile Leu Ser Glu Ile 
            260                 265                 270         


Lys Pro Leu His Asn Phe Leu Thr Gln Leu Gln Pro Phe Leu Lys Leu 
        275                 280                 285             


Lys Gln Lys Gln Ala Asn Leu Val Leu Lys Ile Ile Glu Gln Leu Pro 
    290                 295                 300                 


Ser Ala Lys Glu Ser Pro Asp Lys Phe Leu Glu Val Cys Thr Trp Val 
305                 310                 315                 320 


Asp Gln Ile Ala Ala Leu Asn Asp Ser Lys Thr Arg Lys Thr Thr Ser 
                325                 330                 335     


Glu Thr Val Arg Ala Val Leu Asp Ser Leu Ser Glu Lys Lys Lys Ser 
            340                 345                 350         


Ser Pro 
        


<210>  3
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  3
gataaggatt gtaagacttc atcc                                              24


<210>  4
<211>  354
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  4

Met Asn Thr Lys Tyr Asn Lys Glu Phe Leu Leu Tyr Leu Ala Gly Phe 
1               5                   10                  15      


Val Asp Gly Asp Gly Ser Ile Tyr Ala Thr Ile Met Pro Asn Gln Arg 
            20                  25                  30          


Ala Lys Phe Arg His Val Leu Arg Leu His Phe Asn Val Ser Gln Lys 
        35                  40                  45              


Thr Gln Arg Arg Trp Phe Leu Asp Lys Leu Val Asp Glu Ile Gly Val 
    50                  55                  60                  


Gly Tyr Val Arg Asp Arg Gly Ser Val Ser Asp Tyr Val Leu Ser Glu 
65                  70                  75                  80  


Ile Lys Pro Leu His Asn Phe Leu Thr Gln Leu Gln Pro Phe Leu Lys 
                85                  90                  95      


Leu Lys Gln Lys Gln Ala Asn Leu Val Leu Lys Ile Ile Glu Gln Leu 
            100                 105                 110         


Pro Ser Ala Lys Glu Ser Pro Asp Lys Phe Leu Glu Val Cys Thr Trp 
        115                 120                 125             


Val Asp Gln Ile Ala Ala Leu Asn Asp Ser Lys Thr Arg Lys Thr Thr 
    130                 135                 140                 


Ser Glu Thr Val Arg Ala Val Leu Asp Ser Leu Pro Gly Ser Val Gly 
145                 150                 155                 160 


Gly Leu Ser Pro Ser Gln Ala Ser Ser Ala Ala Ser Ser Ala Ser Ser 
                165                 170                 175     


Ser Pro Gly Ser Gly Ile Ser Glu Ala Leu Arg Ala Gly Ala Gly Ser 
            180                 185                 190         


Gly Thr Gly Tyr Asn Lys Glu Phe Leu Leu Tyr Leu Ala Gly Phe Val 
        195                 200                 205             


Asp Gly Asp Gly Ser Ile Thr Ala Thr Ile Thr Pro Cys Gln Arg Val 
    210                 215                 220                 


Lys Phe Lys His Tyr Leu Gln Leu Arg Phe Asn Val Ser Gln Lys Thr 
225                 230                 235                 240 


Gln Arg Arg Trp Phe Leu Asp Lys Leu Val Asp Glu Ile Gly Val Gly 
                245                 250                 255     


Tyr Val Gln Asp Leu Gly Ser Val Ser Glu Tyr Arg Leu Ser Gln Ile 
            260                 265                 270         


Lys Pro Leu His Asn Phe Leu Thr Gln Leu Gln Pro Phe Leu Lys Leu 
        275                 280                 285             


Lys Gln Lys Gln Ala Asn Leu Val Leu Lys Ile Ile Glu Gln Leu Pro 
    290                 295                 300                 


Ser Ala Lys Glu Ser Pro Asp Lys Phe Leu Glu Val Cys Thr Trp Val 
305                 310                 315                 320 


Asp Gln Ile Ala Ala Leu Asn Asp Ser Lys Thr Arg Lys Thr Thr Ser 
                325                 330                 335     


Glu Thr Val Arg Ala Val Leu Asp Ser Leu Ser Glu Lys Lys Lys Ser 
            340                 345                 350         


Ser Pro 
        


<210>  5
<211>  1062
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  5
atgaatacaa aatataataa agagttctta ctctacttag cagggtttgt agacggtgac       60

ggttccatct atgccactat catgcctaat caacgggcta agttcaggca cgttctgcgg      120

ctccatttca atgtctctca gaagacacag cgccgttggt tcctcgacaa gctggtggac      180

gagatcggtg tgggttacgt gcgggaccgt ggcagcgtct ccgattacgt gctgtccgag      240

atcaagcctt tgcataattt tttaacacaa ctacaacctt ttctaaaact aaaacaaaaa      300

caagcaaatt tagttttaaa aattattgaa caacttccgt cagcaaaaga atccccggac      360

aaattcttag aagtttgtac atgggtggat caaattgcag ctctgaatga ttcgaagacg      420

cgtaaaacaa cttctgaaac cgttcgtgct gtgctagaca gtttaccagg atccgtggga      480

ggtctatcgc catctcaggc atccagcgcc gcatcctcgg cttcctcaag cccgggttca      540

gggatctccg aagcactcag agctggagca ggttccggca ctggatacaa caaggaattc      600

ctgctctacc tggcgggctt cgtcgacggg gacggctcca tcacggccac tatcacgcct      660

tgtcaacgtg tgaagttcaa gcactatctg cagctccgtt tcaatgtctc tcagaagaca      720

cagcgccgtt ggttcctcga caagctggtg gacgagatcg gtgtgggtta cgtgcaggac      780

ctgggcagcg tctccgagta ccgtctgtcc cagatcaagc ctctgcacaa cttcctgacc      840

cagctccagc ccttcctgaa gctcaagcag aagcaggcca acctcgtgct gaagatcatc      900

gagcagctgc cctccgccaa ggaatccccg gacaagttcc tggaggtgtg cacctgggtg      960

gaccagatcg ccgctctgaa cgactccaag acccgcaaga ccacttccga aaccgtccgc     1020

gccgttctag acagtctctc cgagaagaag aagtcgtccc cc                        1062


<210>  6
<211>  35
<212>  PRT
<213>  Homo sapiens


<400>  6

Met Ser Val Leu Thr Pro Leu Leu Leu Arg Gly Leu Thr Gly Ser Ala 
1               5                   10                  15      


Arg Arg Leu Pro Val Pro Arg Ala Lys Ile His Ser Leu Pro Pro Glu 
            20                  25                  30          


Gly Lys Leu 
        35  


<210>  7
<211>  63
<212>  PRT
<213>  Neurospora crassa


<400>  7

Met Ala Ser Thr Arg Val Leu Ala Ser Arg Leu Ala Ser Gln Met Ala 
1               5                   10                  15      


Ala Ser Ala Lys Val Ala Arg Pro Ala Val Arg Val Ala Gln Val Ser 
            20                  25                  30          


Lys Arg Thr Ile Gln Thr Gly Ser Pro Leu Gln Thr Leu Lys Arg Thr 
        35                  40                  45              


Gln Met Thr Ser Ile Val Asn Ala Thr Thr Arg Gln Ala Phe Gln 
    50                  55                  60              


<210>  8
<211>  98
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  8

Met Ser Val Leu Thr Pro Leu Leu Leu Arg Gly Leu Thr Gly Ser Ala 
1               5                   10                  15      


Arg Arg Leu Pro Val Pro Arg Ala Lys Ile His Ser Leu Pro Pro Glu 
            20                  25                  30          


Gly Lys Leu Met Ala Ser Thr Arg Val Leu Ala Ser Arg Leu Ala Ser 
        35                  40                  45              


Gln Met Ala Ala Ser Ala Lys Val Ala Arg Pro Ala Val Arg Val Ala 
    50                  55                  60                  


Gln Val Ser Lys Arg Thr Ile Gln Thr Gly Ser Pro Leu Gln Thr Leu 
65                  70                  75                  80  


Lys Arg Thr Gln Met Thr Ser Ile Val Asn Ala Thr Thr Arg Gln Ala 
                85                  90                  95      


Phe Gln 
        


<210>  9
<211>  18
<212>  PRT
<213>  Mouse parovirus NS2


<400>  9

Val Asp Glu Met Thr Lys Lys Phe Gly Thr Leu Thr Ile His Asp Thr 
1               5                   10                  15      


Glu Lys 
        


<210>  10
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  10

Leu Gly Ala Gly Leu Gly Ala Leu Gly Leu 
1               5                   10  


<210>  11
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  11
ctattcctaa cattctgaag tagg                                              24


<210>  12
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  12
ccaccctagc tatcataagc aca                                               23


<210>  13
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  13
aagcaattga tttgcattca atagatgtag gatgaagtcc tgca                        44


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  14
gcctgaccca tagccataat                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  15
cggctgcgta ttctacgtta                                                   20


<210>  16
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  16
tctcaaccct agcagaaaca accgg                                             25


<210>  17
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  17
cccatgacta ccatcagcaa tag                                               23


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  18
tggaatcgga ccagtaggaa                                                   20


<210>  19
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  19
agtgctgaac tggtgtaggg c                                                 21


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  20
gccgctagag gtgaaattct                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  21
tcggaactac gacggtatct                                                   20


<210>  22
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  22
aagacggacc agagcgaaag cat                                               23


<210>  23
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  23
gacttcatcc tacatctatt g                                                 21


<210>  24
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  24
gtaaggatag taagtcttcc aa                                                22


<210>  25
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  25
tcaaggatag cacgtctcca ac                                                22


<210>  26
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  26
ttaaggatgg taagacagta tc                                                22


<210>  27
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  27
ctaaggttag taagtattca ac                                                22


<210>  28
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  28
ttcaggatgg cacgtcttca tc                                                22


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  29
agccccgggt actccttgtt                                                   20


<210>  30
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  30
ttccttgcag gaacagag                                                     18


<210>  31
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  31
ctgcttgacc acccatt                                                      17


<210>  32
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  32
ccagcaggcc aggtacacc                                                    19


<210>  33
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  33
accgccaagg atgcac                                                       16


<210>  34
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  34
gcgggtggga atggag                                                       16


