                         SEQUENCE LISTING

<110>  BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITY
 
<120>  SYNTHESIS OF 3-HYDROXYPROPIONIC ACID VIA HYDRATION OF 
       ACETYLENECARBOXYLIC ACID

<130>  TEC2021-0095-01PCT // 6550-000410-WO-POA

<160>  93    

<170>  PatentIn version 3.5

<210>  1
<211>  447
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cg10062 (NCBI: MZ369159) from Corynebacterium glutamicum 
       codon-optimized for Escherichia coli

<400>  1
atgccgacct acacctgctg gagccaacgc attcgtatta gccgtgaagc gaagcaacgc       60

atcgcggaag cgattaccga cgcgcaccat gaactggcgc acgcgccgaa gtacctggtg      120

caggttattt tcaacgaagt ggagccggac agctatttta tcgcggcgca gagcgcgagc      180

gagaaccaca tttgggttca agcgaccatc cgtagcggcc gtaccgaaaa gcagaaagag      240

gaactgctgc tgcgtctgac ccaagagatc gcgctgattc tgggtatccc gaacgaggaa      300

gtgtgggttt acattaccga aatcccgggt agcaacatga ccgaatatgg ccgtctgctg      360

atggagccgg gcgaggaaga gaaatggttc aacagcctgc cggagggcct gcgtgagcgt      420

ctgaccgaac tggagggtag cagcgaa                                          447


<210>  2
<211>  447
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cg10062(E114Q) from Corynebacterium glutamicum codon-optimized 
       for Escherichia coli

<400>  2
atgccgacct acacctgctg gagccaacgc attcgtatta gccgtgaagc gaagcaacgc       60

atcgcggaag cgattaccga cgcgcaccat gaactggcgc acgcgccgaa gtacctggtg      120

caggttattt tcaacgaagt ggagccggac agctatttta tcgcggcgca gagcgcgagc      180

gagaaccaca tttgggttca agcgaccatc cgtagcggcc gtaccgaaaa gcagaaagag      240

gaactgctgc tgcgtctgac ccaagagatc gcgctgattc tgggtatccc gaacgaggaa      300

gtgtgggttt acattaccga aatcccgggt agcaacatga cccagtatgg ccgtctgctg      360

atggagccgg gcgaggaaga gaaatggttc aacagcctgc cggagggcct gcgtgagcgt      420

ctgaccgaac tggagggtag cagcgaa                                          447


<210>  3
<211>  447
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cg10062(E114D) from Corynebacterium glutamicum codon-optimized 
       for Escherichia coli

<400>  3
atgccgacct acacctgctg gagccaacgc attcgtatta gccgtgaagc gaagcaacgc       60

atcgcggaag cgattaccga cgcgcaccat gaactggcgc acgcgccgaa gtacctggtg      120

caggttattt tcaacgaagt ggagccggac agctatttta tcgcggcgca gagcgcgagc      180

gagaaccaca tttgggttca agcgaccatc cgtagcggcc gtaccgaaaa gcagaaagag      240

gaactgctgc tgcgtctgac ccaagagatc gcgctgattc tgggtatccc gaacgaggaa      300

gtgtgggttt acattaccga aatcccgggt agcaacatga ccgattatgg ccgtctgctg      360

atggagccgg gcgaggaaga gaaatggttc aacagcctgc cggagggcct gcgtgagcgt      420

ctgaccgaac tggagggtag cagcgaa                                          447


<210>  4
<211>  447
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cg10062(E114N) from Corynebacterium glutamicum codon-optimized 
       for Escherichia coli

<400>  4
atgccgacct acacctgctg gagccaacgc attcgtatta gccgtgaagc gaagcaacgc       60

atcgcggaag cgattaccga cgcgcaccat gaactggcgc acgcgccgaa gtacctggtg      120

caggttattt tcaacgaagt ggagccggac agctatttta tcgcggcgca gagcgcgagc      180

gagaaccaca tttgggttca agcgaccatc cgtagcggcc gtaccgaaaa gcagaaagag      240

gaactgctgc tgcgtctgac ccaagagatc gcgctgattc tgggtatccc gaacgaggaa      300

gtgtgggttt acattaccga aatcccgggt agcaacatga ccaactatgg ccgtctgctg      360

atggagccgg gcgaggaaga gaaatggttc aacagcctgc cggagggcct gcgtgagcgt      420

ctgaccgaac tggagggtag cagcgaa                                          447


<210>  5
<211>  447
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cg10062(H28A) from Corynebacterium glutamicum codon-optimized for
       Escherichia coli

<400>  5
atgccgacct acacctgctg gagccaacgc attcgtatta gccgtgaagc gaagcaacgc       60

atcgcggaag cgattaccga cgcggcgcat gaactggcgc acgcgccgaa gtacctggtg      120

caggttattt tcaacgaagt ggagccggac agctatttta tcgcggcgca gagcgcgagc      180

gagaaccaca tttgggttca agcgaccatc cgtagcggcc gtaccgaaaa gcagaaagag      240

gaactgctgc tgcgtctgac ccaagagatc gcgctgattc tgggtatccc gaacgaggaa      300

gtgtgggttt acattaccga aatcccgggt agcaacatga ccgaatatgg ccgtctgctg      360

atggagccgg gcgaggaaga gaaatggttc aacagcctgc cggagggcct gcgtgagcgt      420

ctgaccgaac tggagggtag cagcgaa                                          447


<210>  6
<211>  447
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cg10062(R70A) from Corynebacterium glutamicum codon-optimized for
       Escherichia coli

<400>  6
atgccgacct acacctgctg gagccaacgc attcgtatta gccgtgaagc gaagcaacgc       60

atcgcggaag cgattaccga cgcgcaccat gaactggcgc acgcgccgaa gtacctggtg      120

caggttattt tcaacgaagt ggagccggac agctatttta tcgcggcgca gagcgcgagc      180

gagaaccaca tttgggttca agcgaccatc gcgagcggcc gtaccgaaaa gcagaaagag      240

gaactgctgc tgcgtctgac ccaagagatc gcgctgattc tgggtatccc gaacgaggaa      300

gtgtgggttt acattaccga aatcccgggt agcaacatga ccgaatatgg ccgtctgctg      360

atggagccgg gcgaggaaga gaaatggttc aacagcctgc cggagggcct gcgtgagcgt      420

ctgaccgaac tggagggtag cagcgaa                                          447


<210>  7
<211>  447
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cg10062(R70K) from Corynebacterium glutamicum codon-optimized for
       Escherichia coli

<400>  7
atgccgacct acacctgctg gagccaacgc attcgtatta gccgtgaagc gaagcaacgc       60

atcgcggaag cgattaccga cgcgcaccat gaactggcgc acgcgccgaa gtacctggtg      120

caggttattt tcaacgaagt ggagccggac agctatttta tcgcggcgca gagcgcgagc      180

gagaaccaca tttgggttca agcgaccatc aaaagcggcc gtaccgaaaa gcagaaagag      240

gaactgctgc tgcgtctgac ccaagagatc gcgctgattc tgggtatccc gaacgaggaa      300

gtgtgggttt acattaccga aatcccgggt agcaacatga ccgaatatgg ccgtctgctg      360

atggagccgg gcgaggaaga gaaatggttc aacagcctgc cggagggcct gcgtgagcgt      420

ctgaccgaac tggagggtag cagcgaa                                          447


<210>  8
<211>  447
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cg10062(R73A) from Corynebacterium glutamicum codon-optimized for
       Escherichia coli

<400>  8
atgccgacct acacctgctg gagccaacgc attcgtatta gccgtgaagc gaagcaacgc       60

atcgcggaag cgattaccga cgcgcaccat gaactggcgc acgcgccgaa gtacctggtg      120

caggttattt tcaacgaagt ggagccggac agctatttta tcgcggcgca gagcgcgagc      180

gagaaccaca tttgggttca agcgaccatc cgtagcggcg cgaccgaaaa gcagaaagag      240

gaactgctgc tgcgtctgac ccaagagatc gcgctgattc tgggtatccc gaacgaggaa      300

gtgtgggttt acattaccga aatcccgggt agcaacatga ccgaatatgg ccgtctgctg      360

atggagccgg gcgaggaaga gaaatggttc aacagcctgc cggagggcct gcgtgagcgt      420

ctgaccgaac tggagggtag cagcgaa                                          447


<210>  9
<211>  447
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cg10062(R73K) from Corynebacterium glutamicum codon-optimized for
       Escherichia coli

<400>  9
atgccgacct acacctgctg gagccaacgc attcgtatta gccgtgaagc gaagcaacgc       60

atcgcggaag cgattaccga cgcgcaccat gaactggcgc acgcgccgaa gtacctggtg      120

caggttattt tcaacgaagt ggagccggac agctatttta tcgcggcgca gagcgcgagc      180

gagaaccaca tttgggttca agcgaccatc cgtagcggca aaaccgaaaa gcagaaagag      240

gaactgctgc tgcgtctgac ccaagagatc gcgctgattc tgggtatccc gaacgaggaa      300

gtgtgggttt acattaccga aatcccgggt agcaacatga ccgaatatgg ccgtctgctg      360

atggagccgg gcgaggaaga gaaatggttc aacagcctgc cggagggcct gcgtgagcgt      420

ctgaccgaac tggagggtag cagcgaa                                          447


<210>  10
<211>  447
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cg10062(Y103A) from Corynebacterium glutamicum codon-optimized 
       for Escherichia coli

<400>  10
atgccgacct acacctgctg gagccaacgc attcgtatta gccgtgaagc gaagcaacgc       60

atcgcggaag cgattaccga cgcgcaccat gaactggcgc acgcgccgaa gtacctggtg      120

caggttattt tcaacgaagt ggagccggac agctatttta tcgcggcgca gagcgcgagc      180

gagaaccaca tttgggttca agcgaccatc cgtagcggcc gtaccgaaaa gcagaaagag      240

gaactgctgc tgcgtctgac ccaagagatc gcgctgattc tgggtatccc gaacgaggaa      300

gtgtgggttg cgattaccga aatcccgggt agcaacatga ccgaatatgg ccgtctgctg      360

atggagccgg gcgaggaaga gaaatggttc aacagcctgc cggagggcct gcgtgagcgt      420

ctgaccgaac tggagggtag cagcgaa                                          447


<210>  11
<211>  447
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cg10062(Y103F) from Corynebacterium glutamicum codon-optimized 
       for Escherichia coli

<400>  11
atgccgacct acacctgctg gagccaacgc attcgtatta gccgtgaagc gaagcaacgc       60

atcgcggaag cgattaccga cgcgcaccat gaactggcgc acgcgccgaa gtacctggtg      120

caggttattt tcaacgaagt ggagccggac agctatttta tcgcggcgca gagcgcgagc      180

gagaaccaca tttgggttca agcgaccatc cgtagcggcc gtaccgaaaa gcagaaagag      240

gaactgctgc tgcgtctgac ccaagagatc gcgctgattc tgggtatccc gaacgaggaa      300

gtgtgggttt ttattaccga aatcccgggt agcaacatga ccgaatatgg ccgtctgctg      360

atggagccgg gcgaggaaga gaaatggttc aacagcctgc cggagggcct gcgtgagcgt      420

ctgaccgaac tggagggtag cagcgaa                                          447


<210>  12
<211>  447
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cg10062(E114A) from Corynebacterium glutamicum codon-optimized 
       for Escherichia coli

<400>  12
atgccgacct acacctgctg gagccaacgc attcgtatta gccgtgaagc gaagcaacgc       60

atcgcggaag cgattaccga cgcgcaccat gaactggcgc acgcgccgaa gtacctggtg      120

caggttattt tcaacgaagt ggagccggac agctatttta tcgcggcgca gagcgcgagc      180

gagaaccaca tttgggttca agcgaccatc cgtagcggcc gtaccgaaaa gcagaaagag      240

gaactgctgc tgcgtctgac ccaagagatc gcgctgattc tgggtatccc gaacgaggaa      300

gtgtgggttt acattaccga aatcccgggt agcaacatga ccgcgtatgg ccgtctgctg      360

atggagccgg gcgaggaaga gaaatggttc aacagcctgc cggagggcct gcgtgagcgt      420

ctgaccgaac tggagggtag cagcgaa                                          447


<210>  13
<211>  447
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cg10062(E114D-Y103F) from Corynebacterium glutamicum 
       codon-optimized for Escherichia coli

<400>  13
atgccgacct acacctgctg gagccaacgc attcgtatta gccgtgaagc gaagcaacgc       60

atcgcggaag cgattaccga cgcgcaccat gaactggcgc acgcgccgaa gtacctggtg      120

caggttattt tcaacgaagt ggagccggac agctatttta tcgcggcgca gagcgcgagc      180

gagaaccaca tttgggttca agcgaccatc cgtagcggcc gtaccgaaaa gcagaaagag      240

gaactgctgc tgcgtctgac ccaagagatc gcgctgattc tgggtatccc gaacgaggaa      300

gtgtgggttt ttattaccga aatcccgggt agcaacatga ccgattatgg ccgtctgctg      360

atggagccgg gcgaggaaga gaaatggttc aacagcctgc cggagggcct gcgtgagcgt      420

ctgaccgaac tggagggtag cagcgaa                                          447


<210>  14
<211>  450
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cis-CaaD from Coryneform sp. codon-optimized for expression in 
       Escherichia coli

<400>  14
atgccggttt acatggttta cgttagccag gaccgtctga ccccgagcgc gaagcacgcg       60

gttgcgaagg cgattaccga tgcgcaccgt ggtctgaccg gcacccagca cttcctggcg      120

caagtgaact ttcaggagca accggcgggt aacgtgttcc tgggtggcgt tcagcaaggt      180

ggcgacacca tctttgttca tggtctgcac cgtgagggcc gtagcgcgga tctgaagggc      240

cagctggcgc aacgtattgt tgacgatgtg agcgttgcgg cggaaatcga ccgtaaacac      300

atttgggtgt acttcggcga gatgccggcg cagcaaatgg ttgaatatgg ccgtttcctg      360

ccgcagccgg gtcatgaggg tgaatggttt gacaacctga gcagcgatga acgtgcgttt      420

atggagacca atgttgatgt gagccgtacc                                       450


<210>  15
<211>  1008
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered phosphite dehydrogenase (PTDH) from Pseudomonas 
       stutzeri

<400>  15
atgctgccga aactcgttat aactcaccga gtacacgaag agatcctgca actgctggcg       60

ccacattgcg agctgataac caaccagacc gacagcacgc tgacgcgcga ggaaattctg      120

cgccgctgtc gcgatgctca ggcgatgatg gcgttcatgc ccgatcgggt cgatgcagac      180

tttcttcaag cctgccctga gctgcgtgta atcggctgcg cgctcaaggg cttcgacaat      240

ttcgatgtgg acgcctgtac tgcccgcggg gtctggctga ccttcgtgcc tgatctgttg      300

acggtcccga ctgccgagct ggcgatcgga ctggcggtgg ggctggggcg gcatctgcgg      360

gcagcagatg cgttcgtccg ctctggcaag ttccggggct ggcaaccacg gttctacggc      420

acggggctgg ataacgctac ggtcggcttc cttggcatgg gcgccatcgg actggccatg      480

gctgatcgct tgcagggatg gggcgcgacc ctgcagtacc acgcggcgaa ggctctggat      540

acacaaaccg agcaacggct cggcctgcgc caggtggcgt gcagcgaact cttcgccagc      600

tcggacttca tcctgctggc gcttcccttg aatgccgata ccctgcatct ggtcaacgcc      660

gagctgcttg ccctcgtacg gccgggcgct ctgcttgtaa acccctgtcg tggctcggta      720

gtggatgaag ccgccgtgct cgcggcgctt gagcgaggcc agctcggcgg gtatgcggcg      780

gatgtattcg aaatggaaga ctgggctcgc gcggaccggc cgcagcagat cgatcctgcg      840

ctgctcgcgc atccgaatac gctgttcact ccgcacatag ggtcggcagt gcgcgcggtg      900

cgcctggaga ttgaacgttg tgcagcgcag aacatcctcc aggcattggc aggtgagcgc      960

ccaatcaacg ctgtgaaccg tctgcccaag gccaatcctg ccgcagac                  1008


<210>  16
<211>  411
<212>  DNA
<213>  Coryneform sp. FG41

<400>  16
atgcccttaa tccgtataga tcttaccagt gatcgttcga gagagcaacg gcgggcgatt       60

gctgatgcag tccatgacgc tttagtagaa gttttagcga ttccggctcg tgatcgcttc      120

cagatactga ctgcgcacga tccctctgat attatagccg aagatgctgg acttggcttt      180

cagcggtccc ccagtgtagt catcatacac gtctttacac aggcaggtag aactattgaa      240

acgaaacaga gagtatttgc agcgataaca gaaagtctgg ctccaatcgg tgttgcagga      300

tctgatgttt ttatcgcaat caccgaaaat gcaccccatg actggagctt tgggtttggc      360

agtgcacaat atgtcacggg tgaacttgcg attccagcca ctggtgcggc t               411


<210>  17
<211>  744
<212>  DNA
<213>  Escherichia coli

<400>  17
atgatcgttt tagtaactgg agcaacggca ggttttggtg aatgcattac tcgtcgtttt       60

attcaacaag ggcataaagt tatcgccact ggccgtcgcc aggaacggtt gcaggagtta      120

aaagacgaac tgggagataa tctgtatatc gcccaactgg acgttcgcaa ccgcgccgct      180

attgaagaga tgctggcatc gcttcctgcc gagtggtgca atattgatat cctggtaaat      240

aatgccggcc tggcgttggg catggagcct gcgcataaag ccagcgttga agactgggaa      300

acgatgattg ataccaacaa caaaggcctg gtatatatga cgcgcgccgt cttaccgggt      360

atggttgaac gtaatcatgg tcatattatt aacattggct caacggcagg tagctggccg      420

tatgccggtg gtaacgttta cggtgcgacg aaagcgtttg ttcgtcagtt tagcctgaat      480

ctgcgtacgg atctgcatgg tacggcggtg cgcgtcaccg acatcgaacc gggtctggtg      540

ggtggtaccg agttttccaa tgtccgcttt aaaggcgatg acggtaaagc agaaaaaacc      600

tatcaaaata ccgttgcatt gacgccagaa gatgtcagcg aagccgtctg gtgggtgtca      660

acgctgcctg ctcacgtcaa tatcaatacc ctggaaatga tgccggttac ccaaagctat      720

gccggactga atgtccaccg tcag                                             744


<210>  18
<211>  885
<212>  DNA
<213>  Pseudomonas putida KT2440

<400>  18
atgcgtatcg cattcatcgg cctgggcaac atgggcgcgc ccatggcccg caacctgatc       60

aaggccgggc atcagctgaa cctgttcgac ctgaacaagg ccgtgctggc cgagctggca      120

gaactgggcg ggcagatcag cccctcgccc aaggacgcgg cggccaacag cgagctggtg      180

atcaccatgc tgccggccgc agcccatgtg cgtagcgtgt acttgaacga ggacggcgta      240

ctggccggta ttcgtcctgg cacgccgacc gttgactgca gcaccatcga cccgcagacc      300

gcacgtgacg tgtccaaggc cgcagcggca aagggcgtgg acatggggga tgcgccggtt      360

tccggtggta ctggcggcgc ggcggccggc accctgacgt tcatggtcgg cgccagtacc      420

gagttgttcg ccagcctcaa gccggtactg gagcagatgg gccgcaacat cgtgcactgc      480

ggggaagtcg gtaccggcca gatcgccaag atctgcaaca acctgctgct cggcatttcg      540

atgatcggcg tgtccgaggc catggccctg ggtaacgcgc tgggtatcga taccaaggtg      600

ctggccggca tcatcaacag ttcgaccggg cgttgctgga gctcggacac ctacaacccg      660

tggccgggca tcatcgaaac cgcacctgca tcgcgtggct acaccggtgg ctttggcgcc      720

gaactcatgc tcaaggacct ggggttggcc accgaagcgg cacgccaggc acaccaaccg      780

gtgattctcg gtgccgtggc ccagcagctg taccaggcca tgagcctgcg aggcgagggt      840

ggcaaggact tctcggccat cgtcgagggt tatcgcaaga aggat                      885


<210>  19
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PTDH N-terminal His6-tag from pET-15b vector

<400>  19
atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat       60


<210>  20
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEV protease recognition site and C-terminal His6-tag

<400>  20
gagaacctgt attttcaagg cctcgagcac caccaccacc accac                       45


<210>  21
<211>  148
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cg10062 from Corynebacterium glutamicum codon-optimized for 
       Escherichia coli

<400>  21

Pro Thr Tyr Thr Cys Trp Ser Gln Arg Ile Arg Ile Ser Arg Glu Ala 
1               5                   10                  15      


Lys Gln Arg Ile Ala Glu Ala Ile Thr Asp Ala His His Glu Leu Ala 
            20                  25                  30          


His Ala Pro Lys Tyr Leu Val Gln Val Ile Phe Asn Glu Val Glu Pro 
        35                  40                  45              


Asp Ser Tyr Phe Ile Ala Ala Gln Ser Ala Ser Glu Asn His Ile Trp 
    50                  55                  60                  


Val Gln Ala Thr Ile Arg Ser Gly Arg Thr Glu Lys Gln Lys Glu Glu 
65                  70                  75                  80  


Leu Leu Leu Arg Leu Thr Gln Glu Ile Ala Leu Ile Leu Gly Ile Pro 
                85                  90                  95      


Asn Glu Glu Val Trp Val Tyr Ile Thr Glu Ile Pro Gly Ser Asn Met 
            100                 105                 110         


Thr Glu Tyr Gly Arg Leu Leu Met Glu Pro Gly Glu Glu Glu Lys Trp 
        115                 120                 125             


Phe Asn Ser Leu Pro Glu Gly Leu Arg Glu Arg Leu Thr Glu Leu Glu 
    130                 135                 140                 


Gly Ser Ser Glu 
145             


<210>  22
<211>  148
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cg10062(E114Q) from Corynebacterium glutamicum codon-optimized 
       for Escherichia coli

<400>  22

Pro Thr Tyr Thr Cys Trp Ser Gln Arg Ile Arg Ile Ser Arg Glu Ala 
1               5                   10                  15      


Lys Gln Arg Ile Ala Glu Ala Ile Thr Asp Ala His His Glu Leu Ala 
            20                  25                  30          


His Ala Pro Lys Tyr Leu Val Gln Val Ile Phe Asn Glu Val Glu Pro 
        35                  40                  45              


Asp Ser Tyr Phe Ile Ala Ala Gln Ser Ala Ser Glu Asn His Ile Trp 
    50                  55                  60                  


Val Gln Ala Thr Ile Arg Ser Gly Arg Thr Glu Lys Gln Lys Glu Glu 
65                  70                  75                  80  


Leu Leu Leu Arg Leu Thr Gln Glu Ile Ala Leu Ile Leu Gly Ile Pro 
                85                  90                  95      


Asn Glu Glu Val Trp Val Tyr Ile Thr Glu Ile Pro Gly Ser Asn Met 
            100                 105                 110         


Thr Gln Tyr Gly Arg Leu Leu Met Glu Pro Gly Glu Glu Glu Lys Trp 
        115                 120                 125             


Phe Asn Ser Leu Pro Glu Gly Leu Arg Glu Arg Leu Thr Glu Leu Glu 
    130                 135                 140                 


Gly Ser Ser Glu 
145             


<210>  23
<211>  148
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cg10062(E114D) from Corynebacterium glutamicum codon-optimized 
       for Escherichia coli

<400>  23

Pro Thr Tyr Thr Cys Trp Ser Gln Arg Ile Arg Ile Ser Arg Glu Ala 
1               5                   10                  15      


Lys Gln Arg Ile Ala Glu Ala Ile Thr Asp Ala His His Glu Leu Ala 
            20                  25                  30          


His Ala Pro Lys Tyr Leu Val Gln Val Ile Phe Asn Glu Val Glu Pro 
        35                  40                  45              


Asp Ser Tyr Phe Ile Ala Ala Gln Ser Ala Ser Glu Asn His Ile Trp 
    50                  55                  60                  


Val Gln Ala Thr Ile Arg Ser Gly Arg Thr Glu Lys Gln Lys Glu Glu 
65                  70                  75                  80  


Leu Leu Leu Arg Leu Thr Gln Glu Ile Ala Leu Ile Leu Gly Ile Pro 
                85                  90                  95      


Asn Glu Glu Val Trp Val Tyr Ile Thr Glu Ile Pro Gly Ser Asn Met 
            100                 105                 110         


Thr Asp Tyr Gly Arg Leu Leu Met Glu Pro Gly Glu Glu Glu Lys Trp 
        115                 120                 125             


Phe Asn Ser Leu Pro Glu Gly Leu Arg Glu Arg Leu Thr Glu Leu Glu 
    130                 135                 140                 


Gly Ser Ser Glu 
145             


<210>  24
<211>  148
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cg10062(E114N) from Corynebacterium glutamicum codon-optimized 
       for Escherichia coli

<400>  24

Pro Thr Tyr Thr Cys Trp Ser Gln Arg Ile Arg Ile Ser Arg Glu Ala 
1               5                   10                  15      


Lys Gln Arg Ile Ala Glu Ala Ile Thr Asp Ala His His Glu Leu Ala 
            20                  25                  30          


His Ala Pro Lys Tyr Leu Val Gln Val Ile Phe Asn Glu Val Glu Pro 
        35                  40                  45              


Asp Ser Tyr Phe Ile Ala Ala Gln Ser Ala Ser Glu Asn His Ile Trp 
    50                  55                  60                  


Val Gln Ala Thr Ile Arg Ser Gly Arg Thr Glu Lys Gln Lys Glu Glu 
65                  70                  75                  80  


Leu Leu Leu Arg Leu Thr Gln Glu Ile Ala Leu Ile Leu Gly Ile Pro 
                85                  90                  95      


Asn Glu Glu Val Trp Val Tyr Ile Thr Glu Ile Pro Gly Ser Asn Met 
            100                 105                 110         


Thr Asn Tyr Gly Arg Leu Leu Met Glu Pro Gly Glu Glu Glu Lys Trp 
        115                 120                 125             


Phe Asn Ser Leu Pro Glu Gly Leu Arg Glu Arg Leu Thr Glu Leu Glu 
    130                 135                 140                 


Gly Ser Ser Glu 
145             


<210>  25
<211>  148
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cg10062(H28A) from Corynebacterium glutamicum codon-optimized for
       Escherichia coli

<400>  25

Pro Thr Tyr Thr Cys Trp Ser Gln Arg Ile Arg Ile Ser Arg Glu Ala 
1               5                   10                  15      


Lys Gln Arg Ile Ala Glu Ala Ile Thr Asp Ala Ala His Glu Leu Ala 
            20                  25                  30          


His Ala Pro Lys Tyr Leu Val Gln Val Ile Phe Asn Glu Val Glu Pro 
        35                  40                  45              


Asp Ser Tyr Phe Ile Ala Ala Gln Ser Ala Ser Glu Asn His Ile Trp 
    50                  55                  60                  


Val Gln Ala Thr Ile Arg Ser Gly Arg Thr Glu Lys Gln Lys Glu Glu 
65                  70                  75                  80  


Leu Leu Leu Arg Leu Thr Gln Glu Ile Ala Leu Ile Leu Gly Ile Pro 
                85                  90                  95      


Asn Glu Glu Val Trp Val Tyr Ile Thr Glu Ile Pro Gly Ser Asn Met 
            100                 105                 110         


Thr Glu Tyr Gly Arg Leu Leu Met Glu Pro Gly Glu Glu Glu Lys Trp 
        115                 120                 125             


Phe Asn Ser Leu Pro Glu Gly Leu Arg Glu Arg Leu Thr Glu Leu Glu 
    130                 135                 140                 


Gly Ser Ser Glu 
145             


<210>  26
<211>  148
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cg10062(R70A) from Corynebacterium glutamicum codon-optimized for
       Escherichia coli

<400>  26

Pro Thr Tyr Thr Cys Trp Ser Gln Arg Ile Arg Ile Ser Arg Glu Ala 
1               5                   10                  15      


Lys Gln Arg Ile Ala Glu Ala Ile Thr Asp Ala His His Glu Leu Ala 
            20                  25                  30          


His Ala Pro Lys Tyr Leu Val Gln Val Ile Phe Asn Glu Val Glu Pro 
        35                  40                  45              


Asp Ser Tyr Phe Ile Ala Ala Gln Ser Ala Ser Glu Asn His Ile Trp 
    50                  55                  60                  


Val Gln Ala Thr Ile Ala Ser Gly Arg Thr Glu Lys Gln Lys Glu Glu 
65                  70                  75                  80  


Leu Leu Leu Arg Leu Thr Gln Glu Ile Ala Leu Ile Leu Gly Ile Pro 
                85                  90                  95      


Asn Glu Glu Val Trp Val Tyr Ile Thr Glu Ile Pro Gly Ser Asn Met 
            100                 105                 110         


Thr Glu Tyr Gly Arg Leu Leu Met Glu Pro Gly Glu Glu Glu Lys Trp 
        115                 120                 125             


Phe Asn Ser Leu Pro Glu Gly Leu Arg Glu Arg Leu Thr Glu Leu Glu 
    130                 135                 140                 


Gly Ser Ser Glu 
145             


<210>  27
<211>  148
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cg10062(R70K) from Corynebacterium glutamicum codon-optimized for
       Escherichia coli

<400>  27

Pro Thr Tyr Thr Cys Trp Ser Gln Arg Ile Arg Ile Ser Arg Glu Ala 
1               5                   10                  15      


Lys Gln Arg Ile Ala Glu Ala Ile Thr Asp Ala His His Glu Leu Ala 
            20                  25                  30          


His Ala Pro Lys Tyr Leu Val Gln Val Ile Phe Asn Glu Val Glu Pro 
        35                  40                  45              


Asp Ser Tyr Phe Ile Ala Ala Gln Ser Ala Ser Glu Asn His Ile Trp 
    50                  55                  60                  


Val Gln Ala Thr Ile Lys Ser Gly Arg Thr Glu Lys Gln Lys Glu Glu 
65                  70                  75                  80  


Leu Leu Leu Arg Leu Thr Gln Glu Ile Ala Leu Ile Leu Gly Ile Pro 
                85                  90                  95      


Asn Glu Glu Val Trp Val Tyr Ile Thr Glu Ile Pro Gly Ser Asn Met 
            100                 105                 110         


Thr Glu Tyr Gly Arg Leu Leu Met Glu Pro Gly Glu Glu Glu Lys Trp 
        115                 120                 125             


Phe Asn Ser Leu Pro Glu Gly Leu Arg Glu Arg Leu Thr Glu Leu Glu 
    130                 135                 140                 


Gly Ser Ser Glu 
145             


<210>  28
<211>  148
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cg10062(R73A) from Corynebacterium glutamicum codon-optimized for
       Escherichia coli

<400>  28

Pro Thr Tyr Thr Cys Trp Ser Gln Arg Ile Arg Ile Ser Arg Glu Ala 
1               5                   10                  15      


Lys Gln Arg Ile Ala Glu Ala Ile Thr Asp Ala His His Glu Leu Ala 
            20                  25                  30          


His Ala Pro Lys Tyr Leu Val Gln Val Ile Phe Asn Glu Val Glu Pro 
        35                  40                  45              


Asp Ser Tyr Phe Ile Ala Ala Gln Ser Ala Ser Glu Asn His Ile Trp 
    50                  55                  60                  


Val Gln Ala Thr Ile Arg Ser Gly Ala Thr Glu Lys Gln Lys Glu Glu 
65                  70                  75                  80  


Leu Leu Leu Arg Leu Thr Gln Glu Ile Ala Leu Ile Leu Gly Ile Pro 
                85                  90                  95      


Asn Glu Glu Val Trp Val Tyr Ile Thr Glu Ile Pro Gly Ser Asn Met 
            100                 105                 110         


Thr Glu Tyr Gly Arg Leu Leu Met Glu Pro Gly Glu Glu Glu Lys Trp 
        115                 120                 125             


Phe Asn Ser Leu Pro Glu Gly Leu Arg Glu Arg Leu Thr Glu Leu Glu 
    130                 135                 140                 


Gly Ser Ser Glu 
145             


<210>  29
<211>  148
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cg10062(R73K) from Corynebacterium glutamicum codon-optimized for
       Escherichia coli

<400>  29

Pro Thr Tyr Thr Cys Trp Ser Gln Arg Ile Arg Ile Ser Arg Glu Ala 
1               5                   10                  15      


Lys Gln Arg Ile Ala Glu Ala Ile Thr Asp Ala His His Glu Leu Ala 
            20                  25                  30          


His Ala Pro Lys Tyr Leu Val Gln Val Ile Phe Asn Glu Val Glu Pro 
        35                  40                  45              


Asp Ser Tyr Phe Ile Ala Ala Gln Ser Ala Ser Glu Asn His Ile Trp 
    50                  55                  60                  


Val Gln Ala Thr Ile Arg Ser Gly Lys Thr Glu Lys Gln Lys Glu Glu 
65                  70                  75                  80  


Leu Leu Leu Arg Leu Thr Gln Glu Ile Ala Leu Ile Leu Gly Ile Pro 
                85                  90                  95      


Asn Glu Glu Val Trp Val Tyr Ile Thr Glu Ile Pro Gly Ser Asn Met 
            100                 105                 110         


Thr Glu Tyr Gly Arg Leu Leu Met Glu Pro Gly Glu Glu Glu Lys Trp 
        115                 120                 125             


Phe Asn Ser Leu Pro Glu Gly Leu Arg Glu Arg Leu Thr Glu Leu Glu 
    130                 135                 140                 


Gly Ser Ser Glu 
145             


<210>  30
<211>  148
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cg10062(Y103A) from Corynebacterium glutamicum codon-optimized 
       for Escherichia coli

<400>  30

Pro Thr Tyr Thr Cys Trp Ser Gln Arg Ile Arg Ile Ser Arg Glu Ala 
1               5                   10                  15      


Lys Gln Arg Ile Ala Glu Ala Ile Thr Asp Ala His His Glu Leu Ala 
            20                  25                  30          


His Ala Pro Lys Tyr Leu Val Gln Val Ile Phe Asn Glu Val Glu Pro 
        35                  40                  45              


Asp Ser Tyr Phe Ile Ala Ala Gln Ser Ala Ser Glu Asn His Ile Trp 
    50                  55                  60                  


Val Gln Ala Thr Ile Arg Ser Gly Arg Thr Glu Lys Gln Lys Glu Glu 
65                  70                  75                  80  


Leu Leu Leu Arg Leu Thr Gln Glu Ile Ala Leu Ile Leu Gly Ile Pro 
                85                  90                  95      


Asn Glu Glu Val Trp Val Ala Ile Thr Glu Ile Pro Gly Ser Asn Met 
            100                 105                 110         


Thr Glu Tyr Gly Arg Leu Leu Met Glu Pro Gly Glu Glu Glu Lys Trp 
        115                 120                 125             


Phe Asn Ser Leu Pro Glu Gly Leu Arg Glu Arg Leu Thr Glu Leu Glu 
    130                 135                 140                 


Gly Ser Ser Glu 
145             


<210>  31
<211>  148
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cg10062(Y103F) from Corynebacterium glutamicum codon-optimized 
       for Escherichia coli

<400>  31

Pro Thr Tyr Thr Cys Trp Ser Gln Arg Ile Arg Ile Ser Arg Glu Ala 
1               5                   10                  15      


Lys Gln Arg Ile Ala Glu Ala Ile Thr Asp Ala His His Glu Leu Ala 
            20                  25                  30          


His Ala Pro Lys Tyr Leu Val Gln Val Ile Phe Asn Glu Val Glu Pro 
        35                  40                  45              


Asp Ser Tyr Phe Ile Ala Ala Gln Ser Ala Ser Glu Asn His Ile Trp 
    50                  55                  60                  


Val Gln Ala Thr Ile Arg Ser Gly Arg Thr Glu Lys Gln Lys Glu Glu 
65                  70                  75                  80  


Leu Leu Leu Arg Leu Thr Gln Glu Ile Ala Leu Ile Leu Gly Ile Pro 
                85                  90                  95      


Asn Glu Glu Val Trp Val Phe Ile Thr Glu Ile Pro Gly Ser Asn Met 
            100                 105                 110         


Thr Glu Tyr Gly Arg Leu Leu Met Glu Pro Gly Glu Glu Glu Lys Trp 
        115                 120                 125             


Phe Asn Ser Leu Pro Glu Gly Leu Arg Glu Arg Leu Thr Glu Leu Glu 
    130                 135                 140                 


Gly Ser Ser Glu 
145             


<210>  32
<211>  148
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cg10062(E114A) from Corynebacterium glutamicum codon-optimized 
       for Escherichia coli

<400>  32

Pro Thr Tyr Thr Cys Trp Ser Gln Arg Ile Arg Ile Ser Arg Glu Ala 
1               5                   10                  15      


Lys Gln Arg Ile Ala Glu Ala Ile Thr Asp Ala His His Glu Leu Ala 
            20                  25                  30          


His Ala Pro Lys Tyr Leu Val Gln Val Ile Phe Asn Glu Val Glu Pro 
        35                  40                  45              


Asp Ser Tyr Phe Ile Ala Ala Gln Ser Ala Ser Glu Asn His Ile Trp 
    50                  55                  60                  


Val Gln Ala Thr Ile Arg Ser Gly Arg Thr Glu Lys Gln Lys Glu Glu 
65                  70                  75                  80  


Leu Leu Leu Arg Leu Thr Gln Glu Ile Ala Leu Ile Leu Gly Ile Pro 
                85                  90                  95      


Asn Glu Glu Val Trp Val Tyr Ile Thr Glu Ile Pro Gly Ser Asn Met 
            100                 105                 110         


Thr Ala Tyr Gly Arg Leu Leu Met Glu Pro Gly Glu Glu Glu Lys Trp 
        115                 120                 125             


Phe Asn Ser Leu Pro Glu Gly Leu Arg Glu Arg Leu Thr Glu Leu Glu 
    130                 135                 140                 


Gly Ser Ser Glu 
145             


<210>  33
<211>  148
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cg10062(E114D-Y103F) from Corynebacterium glutamicum 
       codon-optimized for Escherichia coli

<400>  33

Pro Thr Tyr Thr Cys Trp Ser Gln Arg Ile Arg Ile Ser Arg Glu Ala 
1               5                   10                  15      


Lys Gln Arg Ile Ala Glu Ala Ile Thr Asp Ala His His Glu Leu Ala 
            20                  25                  30          


His Ala Pro Lys Tyr Leu Val Gln Val Ile Phe Asn Glu Val Glu Pro 
        35                  40                  45              


Asp Ser Tyr Phe Ile Ala Ala Gln Ser Ala Ser Glu Asn His Ile Trp 
    50                  55                  60                  


Val Gln Ala Thr Ile Arg Ser Gly Arg Thr Glu Lys Gln Lys Glu Glu 
65                  70                  75                  80  


Leu Leu Leu Arg Leu Thr Gln Glu Ile Ala Leu Ile Leu Gly Ile Pro 
                85                  90                  95      


Asn Glu Glu Val Trp Val Phe Ile Thr Glu Ile Pro Gly Ser Asn Met 
            100                 105                 110         


Thr Asp Tyr Gly Arg Leu Leu Met Glu Pro Gly Glu Glu Glu Lys Trp 
        115                 120                 125             


Phe Asn Ser Leu Pro Glu Gly Leu Arg Glu Arg Leu Thr Glu Leu Glu 
    130                 135                 140                 


Gly Ser Ser Glu 
145             


<210>  34
<211>  149
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cis-CaaD from Coryneform sp. codon-optimized for expression in 
       Escherichia coli

<400>  34

Pro Val Tyr Met Val Tyr Val Ser Gln Asp Arg Leu Thr Pro Ser Ala 
1               5                   10                  15      


Lys His Ala Val Ala Lys Ala Ile Thr Asp Ala His Arg Gly Leu Thr 
            20                  25                  30          


Gly Thr Gln His Phe Leu Ala Gln Val Asn Phe Gln Glu Gln Pro Ala 
        35                  40                  45              


Gly Asn Val Phe Leu Gly Gly Val Gln Gln Gly Gly Asp Thr Ile Phe 
    50                  55                  60                  


Val His Gly Leu His Arg Glu Gly Arg Ser Ala Asp Leu Lys Gly Gln 
65                  70                  75                  80  


Leu Ala Gln Arg Ile Val Asp Asp Val Ser Val Ala Ala Glu Ile Asp 
                85                  90                  95      


Arg Lys His Ile Trp Val Tyr Phe Gly Glu Met Pro Ala Gln Gln Met 
            100                 105                 110         


Val Glu Tyr Gly Arg Phe Leu Pro Gln Pro Gly His Glu Gly Glu Trp 
        115                 120                 125             


Phe Asp Asn Leu Ser Ser Asp Glu Arg Ala Phe Met Glu Thr Asn Val 
    130                 135                 140                 


Asp Val Ser Arg Thr 
145                 


<210>  35
<211>  336
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered phosphite dehydrogenase (PTDH) from Pseudomonas 
       stutzeri

<400>  35

Met Leu Pro Lys Leu Val Ile Thr His Arg Val His Glu Glu Ile Leu 
1               5                   10                  15      


Gln Leu Leu Ala Pro His Cys Glu Leu Ile Thr Asn Gln Thr Asp Ser 
            20                  25                  30          


Thr Leu Thr Arg Glu Glu Ile Leu Arg Arg Cys Arg Asp Ala Gln Ala 
        35                  40                  45              


Met Met Ala Phe Met Pro Asp Arg Val Asp Ala Asp Phe Leu Gln Ala 
    50                  55                  60                  


Cys Pro Glu Leu Arg Val Ile Gly Cys Ala Leu Lys Gly Phe Asp Asn 
65                  70                  75                  80  


Phe Asp Val Asp Ala Cys Thr Ala Arg Gly Val Trp Leu Thr Phe Val 
                85                  90                  95      


Pro Asp Leu Leu Thr Val Pro Thr Ala Glu Leu Ala Ile Gly Leu Ala 
            100                 105                 110         


Val Gly Leu Gly Arg His Leu Arg Ala Ala Asp Ala Phe Val Arg Ser 
        115                 120                 125             


Gly Lys Phe Arg Gly Trp Gln Pro Arg Phe Tyr Gly Thr Gly Leu Asp 
    130                 135                 140                 


Asn Ala Thr Val Gly Phe Leu Gly Met Gly Ala Ile Gly Leu Ala Met 
145                 150                 155                 160 


Ala Asp Arg Leu Gln Gly Trp Gly Ala Thr Leu Gln Tyr His Ala Ala 
                165                 170                 175     


Lys Ala Leu Asp Thr Gln Thr Glu Gln Arg Leu Gly Leu Arg Gln Val 
            180                 185                 190         


Ala Cys Ser Glu Leu Phe Ala Ser Ser Asp Phe Ile Leu Leu Ala Leu 
        195                 200                 205             


Pro Leu Asn Ala Asp Thr Leu His Leu Val Asn Ala Glu Leu Leu Ala 
    210                 215                 220                 


Leu Val Arg Pro Gly Ala Leu Leu Val Asn Pro Cys Arg Gly Ser Val 
225                 230                 235                 240 


Val Asp Glu Ala Ala Val Leu Ala Ala Leu Glu Arg Gly Gln Leu Gly 
                245                 250                 255     


Gly Tyr Ala Ala Asp Val Phe Glu Met Glu Asp Trp Ala Arg Ala Asp 
            260                 265                 270         


Arg Pro Gln Gln Ile Asp Pro Ala Leu Leu Ala His Pro Asn Thr Leu 
        275                 280                 285             


Phe Thr Pro His Ile Gly Ser Ala Val Arg Ala Val Arg Leu Glu Ile 
    290                 295                 300                 


Glu Arg Cys Ala Ala Gln Asn Ile Leu Gln Ala Leu Ala Gly Glu Arg 
305                 310                 315                 320 


Pro Ile Asn Ala Val Asn Arg Leu Pro Lys Ala Asn Pro Ala Ala Asp 
                325                 330                 335     


<210>  36
<211>  136
<212>  PRT
<213>  Coryneform sp. FG41

<400>  36

Pro Leu Ile Arg Ile Asp Leu Thr Ser Asp Arg Ser Arg Glu Gln Arg 
1               5                   10                  15      


Arg Ala Ile Ala Asp Ala Val His Asp Ala Leu Val Glu Val Leu Ala 
            20                  25                  30          


Ile Pro Ala Arg Asp Arg Phe Gln Ile Leu Thr Ala His Asp Pro Ser 
        35                  40                  45              


Asp Ile Ile Ala Glu Asp Ala Gly Leu Gly Phe Gln Arg Ser Pro Ser 
    50                  55                  60                  


Val Val Ile Ile His Val Phe Thr Gln Ala Gly Arg Thr Ile Glu Thr 
65                  70                  75                  80  


Lys Gln Arg Val Phe Ala Ala Ile Thr Glu Ser Leu Ala Pro Ile Gly 
                85                  90                  95      


Val Ala Gly Ser Asp Val Phe Ile Ala Ile Thr Glu Asn Ala Pro His 
            100                 105                 110         


Asp Trp Ser Phe Gly Phe Gly Ser Ala Gln Tyr Val Thr Gly Glu Leu 
        115                 120                 125             


Ala Ile Pro Ala Thr Gly Ala Ala 
    130                 135     


<210>  37
<211>  248
<212>  PRT
<213>  Escherichia coli

<400>  37

Met Ile Val Leu Val Thr Gly Ala Thr Ala Gly Phe Gly Glu Cys Ile 
1               5                   10                  15      


Thr Arg Arg Phe Ile Gln Gln Gly His Lys Val Ile Ala Thr Gly Arg 
            20                  25                  30          


Arg Gln Glu Arg Leu Gln Glu Leu Lys Asp Glu Leu Gly Asp Asn Leu 
        35                  40                  45              


Tyr Ile Ala Gln Leu Asp Val Arg Asn Arg Ala Ala Ile Glu Glu Met 
    50                  55                  60                  


Leu Ala Ser Leu Pro Ala Glu Trp Cys Asn Ile Asp Ile Leu Val Asn 
65                  70                  75                  80  


Asn Ala Gly Leu Ala Leu Gly Met Glu Pro Ala His Lys Ala Ser Val 
                85                  90                  95      


Glu Asp Trp Glu Thr Met Ile Asp Thr Asn Asn Lys Gly Leu Val Tyr 
            100                 105                 110         


Met Thr Arg Ala Val Leu Pro Gly Met Val Glu Arg Asn His Gly His 
        115                 120                 125             


Ile Ile Asn Ile Gly Ser Thr Ala Gly Ser Trp Pro Tyr Ala Gly Gly 
    130                 135                 140                 


Asn Val Tyr Gly Ala Thr Lys Ala Phe Val Arg Gln Phe Ser Leu Asn 
145                 150                 155                 160 


Leu Arg Thr Asp Leu His Gly Thr Ala Val Arg Val Thr Asp Ile Glu 
                165                 170                 175     


Pro Gly Leu Val Gly Gly Thr Glu Phe Ser Asn Val Arg Phe Lys Gly 
            180                 185                 190         


Asp Asp Gly Lys Ala Glu Lys Thr Tyr Gln Asn Thr Val Ala Leu Thr 
        195                 200                 205             


Pro Glu Asp Val Ser Glu Ala Val Trp Trp Val Ser Thr Leu Pro Ala 
    210                 215                 220                 


His Val Asn Ile Asn Thr Leu Glu Met Met Pro Val Thr Gln Ser Tyr 
225                 230                 235                 240 


Ala Gly Leu Asn Val His Arg Gln 
                245             


<210>  38
<211>  295
<212>  PRT
<213>  Pseudomonas putida KT2440

<400>  38

Met Arg Ile Ala Phe Ile Gly Leu Gly Asn Met Gly Ala Pro Met Ala 
1               5                   10                  15      


Arg Asn Leu Ile Lys Ala Gly His Gln Leu Asn Leu Phe Asp Leu Asn 
            20                  25                  30          


Lys Ala Val Leu Ala Glu Leu Ala Glu Leu Gly Gly Gln Ile Ser Pro 
        35                  40                  45              


Ser Pro Lys Asp Ala Ala Ala Asn Ser Glu Leu Val Ile Thr Met Leu 
    50                  55                  60                  


Pro Ala Ala Ala His Val Arg Ser Val Tyr Leu Asn Glu Asp Gly Val 
65                  70                  75                  80  


Leu Ala Gly Ile Arg Pro Gly Thr Pro Thr Val Asp Cys Ser Thr Ile 
                85                  90                  95      


Asp Pro Gln Thr Ala Arg Asp Val Ser Lys Ala Ala Ala Ala Lys Gly 
            100                 105                 110         


Val Asp Met Gly Asp Ala Pro Val Ser Gly Gly Thr Gly Gly Ala Ala 
        115                 120                 125             


Ala Gly Thr Leu Thr Phe Met Val Gly Ala Ser Thr Glu Leu Phe Ala 
    130                 135                 140                 


Ser Leu Lys Pro Val Leu Glu Gln Met Gly Arg Asn Ile Val His Cys 
145                 150                 155                 160 


Gly Glu Val Gly Thr Gly Gln Ile Ala Lys Ile Cys Asn Asn Leu Leu 
                165                 170                 175     


Leu Gly Ile Ser Met Ile Gly Val Ser Glu Ala Met Ala Leu Gly Asn 
            180                 185                 190         


Ala Leu Gly Ile Asp Thr Lys Val Leu Ala Gly Ile Ile Asn Ser Ser 
        195                 200                 205             


Thr Gly Arg Cys Trp Ser Ser Asp Thr Tyr Asn Pro Trp Pro Gly Ile 
    210                 215                 220                 


Ile Glu Thr Ala Pro Ala Ser Arg Gly Tyr Thr Gly Gly Phe Gly Ala 
225                 230                 235                 240 


Glu Leu Met Leu Lys Asp Leu Gly Leu Ala Thr Glu Ala Ala Arg Gln 
                245                 250                 255     


Ala His Gln Pro Val Ile Leu Gly Ala Val Ala Gln Gln Leu Tyr Gln 
            260                 265                 270         


Ala Met Ser Leu Arg Gly Glu Gly Gly Lys Asp Phe Ser Ala Ile Val 
        275                 280                 285             


Glu Gly Tyr Arg Lys Lys Asp 
    290                 295 


<210>  39
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PTDH N-terminal His6-tag from pET-15b vector

<400>  39

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His 
            20  


<210>  40
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TEV protease recognition site and C-terminal His6-tag

<400>  40

Glu Asn Leu Tyr Phe Gln Gly Leu Glu His His His His His His 
1               5                   10                  15  


<210>  41
<211>  492
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cg10062 (NCBI: MZ369159) from Corynebacterium glutamicum 
       codon-optimized for Escherichia coli plus TEV protease 
       recognition site and C-terminal His6-tag

<400>  41
atgccgacct acacctgctg gagccaacgc attcgtatta gccgtgaagc gaagcaacgc       60

atcgcggaag cgattaccga cgcgcaccat gaactggcgc acgcgccgaa gtacctggtg      120

caggttattt tcaacgaagt ggagccggac agctatttta tcgcggcgca gagcgcgagc      180

gagaaccaca tttgggttca agcgaccatc cgtagcggcc gtaccgaaaa gcagaaagag      240

gaactgctgc tgcgtctgac ccaagagatc gcgctgattc tgggtatccc gaacgaggaa      300

gtgtgggttt acattaccga aatcccgggt agcaacatga ccgaatatgg ccgtctgctg      360

atggagccgg gcgaggaaga gaaatggttc aacagcctgc cggagggcct gcgtgagcgt      420

ctgaccgaac tggagggtag cagcgaagag aacctgtatt ttcaaggcct cgagcaccac      480

caccaccacc ac                                                          492


<210>  42
<211>  492
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cg10062(E114Q) from Corynebacterium glutamicum codon-optimized 
       for Escherichia coli plus TEV protease recognition site and 
       C-terminal His6-tag

<400>  42
atgccgacct acacctgctg gagccaacgc attcgtatta gccgtgaagc gaagcaacgc       60

atcgcggaag cgattaccga cgcgcaccat gaactggcgc acgcgccgaa gtacctggtg      120

caggttattt tcaacgaagt ggagccggac agctatttta tcgcggcgca gagcgcgagc      180

gagaaccaca tttgggttca agcgaccatc cgtagcggcc gtaccgaaaa gcagaaagag      240

gaactgctgc tgcgtctgac ccaagagatc gcgctgattc tgggtatccc gaacgaggaa      300

gtgtgggttt acattaccga aatcccgggt agcaacatga cccagtatgg ccgtctgctg      360

atggagccgg gcgaggaaga gaaatggttc aacagcctgc cggagggcct gcgtgagcgt      420

ctgaccgaac tggagggtag cagcgaagag aacctgtatt ttcaaggcct cgagcaccac      480

caccaccacc ac                                                          492


<210>  43
<211>  492
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cg10062(E114D) from Corynebacterium glutamicum codon-optimized 
       for Escherichia coli plus TEV protease recognition site and 
       C-terminal His6-tag

<400>  43
atgccgacct acacctgctg gagccaacgc attcgtatta gccgtgaagc gaagcaacgc       60

atcgcggaag cgattaccga cgcgcaccat gaactggcgc acgcgccgaa gtacctggtg      120

caggttattt tcaacgaagt ggagccggac agctatttta tcgcggcgca gagcgcgagc      180

gagaaccaca tttgggttca agcgaccatc cgtagcggcc gtaccgaaaa gcagaaagag      240

gaactgctgc tgcgtctgac ccaagagatc gcgctgattc tgggtatccc gaacgaggaa      300

gtgtgggttt acattaccga aatcccgggt agcaacatga ccgattatgg ccgtctgctg      360

atggagccgg gcgaggaaga gaaatggttc aacagcctgc cggagggcct gcgtgagcgt      420

ctgaccgaac tggagggtag cagcgaagag aacctgtatt ttcaaggcct cgagcaccac      480

caccaccacc ac                                                          492


<210>  44
<211>  492
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cg10062(E114N) from Corynebacterium glutamicum codon-optimized 
       for Escherichia coli plus TEV protease recognition site and 
       C-terminal His6-tag

<400>  44
atgccgacct acacctgctg gagccaacgc attcgtatta gccgtgaagc gaagcaacgc       60

atcgcggaag cgattaccga cgcgcaccat gaactggcgc acgcgccgaa gtacctggtg      120

caggttattt tcaacgaagt ggagccggac agctatttta tcgcggcgca gagcgcgagc      180

gagaaccaca tttgggttca agcgaccatc cgtagcggcc gtaccgaaaa gcagaaagag      240

gaactgctgc tgcgtctgac ccaagagatc gcgctgattc tgggtatccc gaacgaggaa      300

gtgtgggttt acattaccga aatcccgggt agcaacatga ccaactatgg ccgtctgctg      360

atggagccgg gcgaggaaga gaaatggttc aacagcctgc cggagggcct gcgtgagcgt      420

ctgaccgaac tggagggtag cagcgaagag aacctgtatt ttcaaggcct cgagcaccac      480

caccaccacc ac                                                          492


<210>  45
<211>  492
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cg10062(H28A) from Corynebacterium glutamicum codon-optimized for
       Escherichia coli plus TEV protease recognition site and 
       C-terminal His6-tag

<400>  45
atgccgacct acacctgctg gagccaacgc attcgtatta gccgtgaagc gaagcaacgc       60

atcgcggaag cgattaccga cgcggcgcat gaactggcgc acgcgccgaa gtacctggtg      120

caggttattt tcaacgaagt ggagccggac agctatttta tcgcggcgca gagcgcgagc      180

gagaaccaca tttgggttca agcgaccatc cgtagcggcc gtaccgaaaa gcagaaagag      240

gaactgctgc tgcgtctgac ccaagagatc gcgctgattc tgggtatccc gaacgaggaa      300

gtgtgggttt acattaccga aatcccgggt agcaacatga ccgaatatgg ccgtctgctg      360

atggagccgg gcgaggaaga gaaatggttc aacagcctgc cggagggcct gcgtgagcgt      420

ctgaccgaac tggagggtag cagcgaagag aacctgtatt ttcaaggcct cgagcaccac      480

caccaccacc ac                                                          492


<210>  46
<211>  492
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cg10062(R70A) from Corynebacterium glutamicum codon-optimized for
       Escherichia coli plus TEV protease recognition site and 
       C-terminal His6-tag

<400>  46
atgccgacct acacctgctg gagccaacgc attcgtatta gccgtgaagc gaagcaacgc       60

atcgcggaag cgattaccga cgcgcaccat gaactggcgc acgcgccgaa gtacctggtg      120

caggttattt tcaacgaagt ggagccggac agctatttta tcgcggcgca gagcgcgagc      180

gagaaccaca tttgggttca agcgaccatc gcgagcggcc gtaccgaaaa gcagaaagag      240

gaactgctgc tgcgtctgac ccaagagatc gcgctgattc tgggtatccc gaacgaggaa      300

gtgtgggttt acattaccga aatcccgggt agcaacatga ccgaatatgg ccgtctgctg      360

atggagccgg gcgaggaaga gaaatggttc aacagcctgc cggagggcct gcgtgagcgt      420

ctgaccgaac tggagggtag cagcgaagag aacctgtatt ttcaaggcct cgagcaccac      480

caccaccacc ac                                                          492


<210>  47
<211>  492
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cg10062(R70K) from Corynebacterium glutamicum codon-optimized for
       Escherichia coli plus TEV protease recognition site and 
       C-terminal His6-tag

<400>  47
atgccgacct acacctgctg gagccaacgc attcgtatta gccgtgaagc gaagcaacgc       60

atcgcggaag cgattaccga cgcgcaccat gaactggcgc acgcgccgaa gtacctggtg      120

caggttattt tcaacgaagt ggagccggac agctatttta tcgcggcgca gagcgcgagc      180

gagaaccaca tttgggttca agcgaccatc aaaagcggcc gtaccgaaaa gcagaaagag      240

gaactgctgc tgcgtctgac ccaagagatc gcgctgattc tgggtatccc gaacgaggaa      300

gtgtgggttt acattaccga aatcccgggt agcaacatga ccgaatatgg ccgtctgctg      360

atggagccgg gcgaggaaga gaaatggttc aacagcctgc cggagggcct gcgtgagcgt      420

ctgaccgaac tggagggtag cagcgaagag aacctgtatt ttcaaggcct cgagcaccac      480

caccaccacc ac                                                          492


<210>  48
<211>  492
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cg10062(R73A) from Corynebacterium glutamicum codon-optimized for
       Escherichia coli plus TEV protease recognition site and 
       C-terminal His6-tag

<400>  48
atgccgacct acacctgctg gagccaacgc attcgtatta gccgtgaagc gaagcaacgc       60

atcgcggaag cgattaccga cgcgcaccat gaactggcgc acgcgccgaa gtacctggtg      120

caggttattt tcaacgaagt ggagccggac agctatttta tcgcggcgca gagcgcgagc      180

gagaaccaca tttgggttca agcgaccatc cgtagcggcg cgaccgaaaa gcagaaagag      240

gaactgctgc tgcgtctgac ccaagagatc gcgctgattc tgggtatccc gaacgaggaa      300

gtgtgggttt acattaccga aatcccgggt agcaacatga ccgaatatgg ccgtctgctg      360

atggagccgg gcgaggaaga gaaatggttc aacagcctgc cggagggcct gcgtgagcgt      420

ctgaccgaac tggagggtag cagcgaagag aacctgtatt ttcaaggcct cgagcaccac      480

caccaccacc ac                                                          492


<210>  49
<211>  492
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cg10062(R73K) from Corynebacterium glutamicum codon-optimized for
       Escherichia coli plus TEV protease recognition site and 
       C-terminal His6-tag

<400>  49
atgccgacct acacctgctg gagccaacgc attcgtatta gccgtgaagc gaagcaacgc       60

atcgcggaag cgattaccga cgcgcaccat gaactggcgc acgcgccgaa gtacctggtg      120

caggttattt tcaacgaagt ggagccggac agctatttta tcgcggcgca gagcgcgagc      180

gagaaccaca tttgggttca agcgaccatc cgtagcggca aaaccgaaaa gcagaaagag      240

gaactgctgc tgcgtctgac ccaagagatc gcgctgattc tgggtatccc gaacgaggaa      300

gtgtgggttt acattaccga aatcccgggt agcaacatga ccgaatatgg ccgtctgctg      360

atggagccgg gcgaggaaga gaaatggttc aacagcctgc cggagggcct gcgtgagcgt      420

ctgaccgaac tggagggtag cagcgaagag aacctgtatt ttcaaggcct cgagcaccac      480

caccaccacc ac                                                          492


<210>  50
<211>  492
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cg10062(Y103A) from Corynebacterium glutamicum codon-optimized 
       for Escherichia coli plus TEV protease recognition site and 
       C-terminal His6-tag

<400>  50
atgccgacct acacctgctg gagccaacgc attcgtatta gccgtgaagc gaagcaacgc       60

atcgcggaag cgattaccga cgcgcaccat gaactggcgc acgcgccgaa gtacctggtg      120

caggttattt tcaacgaagt ggagccggac agctatttta tcgcggcgca gagcgcgagc      180

gagaaccaca tttgggttca agcgaccatc cgtagcggcc gtaccgaaaa gcagaaagag      240

gaactgctgc tgcgtctgac ccaagagatc gcgctgattc tgggtatccc gaacgaggaa      300

gtgtgggttg cgattaccga aatcccgggt agcaacatga ccgaatatgg ccgtctgctg      360

atggagccgg gcgaggaaga gaaatggttc aacagcctgc cggagggcct gcgtgagcgt      420

ctgaccgaac tggagggtag cagcgaagag aacctgtatt ttcaaggcct cgagcaccac      480

caccaccacc ac                                                          492


<210>  51
<211>  492
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cg10062(Y103F) from Corynebacterium glutamicum codon-optimized 
       for Escherichia coli plus TEV protease recognition site and 
       C-terminal His6-tag

<400>  51
atgccgacct acacctgctg gagccaacgc attcgtatta gccgtgaagc gaagcaacgc       60

atcgcggaag cgattaccga cgcgcaccat gaactggcgc acgcgccgaa gtacctggtg      120

caggttattt tcaacgaagt ggagccggac agctatttta tcgcggcgca gagcgcgagc      180

gagaaccaca tttgggttca agcgaccatc cgtagcggcc gtaccgaaaa gcagaaagag      240

gaactgctgc tgcgtctgac ccaagagatc gcgctgattc tgggtatccc gaacgaggaa      300

gtgtgggttt ttattaccga aatcccgggt agcaacatga ccgaatatgg ccgtctgctg      360

atggagccgg gcgaggaaga gaaatggttc aacagcctgc cggagggcct gcgtgagcgt      420

ctgaccgaac tggagggtag cagcgaagag aacctgtatt ttcaaggcct cgagcaccac      480

caccaccacc ac                                                          492


<210>  52
<211>  492
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cg10062(E114A) from Corynebacterium glutamicum codon-optimized 
       for Escherichia coli plus TEV protease recognition site and 
       C-terminal His6-tag

<400>  52
atgccgacct acacctgctg gagccaacgc attcgtatta gccgtgaagc gaagcaacgc       60

atcgcggaag cgattaccga cgcgcaccat gaactggcgc acgcgccgaa gtacctggtg      120

caggttattt tcaacgaagt ggagccggac agctatttta tcgcggcgca gagcgcgagc      180

gagaaccaca tttgggttca agcgaccatc cgtagcggcc gtaccgaaaa gcagaaagag      240

gaactgctgc tgcgtctgac ccaagagatc gcgctgattc tgggtatccc gaacgaggaa      300

gtgtgggttt acattaccga aatcccgggt agcaacatga ccgcgtatgg ccgtctgctg      360

atggagccgg gcgaggaaga gaaatggttc aacagcctgc cggagggcct gcgtgagcgt      420

ctgaccgaac tggagggtag cagcgaagag aacctgtatt ttcaaggcct cgagcaccac      480

caccaccacc ac                                                          492


<210>  53
<211>  492
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cg10062(E114D-Y103F) from Corynebacterium glutamicum 
       codon-optimized for Escherichia coli plus TEV protease 
       recognition site and C-terminal His6-tag

<400>  53
atgccgacct acacctgctg gagccaacgc attcgtatta gccgtgaagc gaagcaacgc       60

atcgcggaag cgattaccga cgcgcaccat gaactggcgc acgcgccgaa gtacctggtg      120

caggttattt tcaacgaagt ggagccggac agctatttta tcgcggcgca gagcgcgagc      180

gagaaccaca tttgggttca agcgaccatc cgtagcggcc gtaccgaaaa gcagaaagag      240

gaactgctgc tgcgtctgac ccaagagatc gcgctgattc tgggtatccc gaacgaggaa      300

gtgtgggttt ttattaccga aatcccgggt agcaacatga ccgattatgg ccgtctgctg      360

atggagccgg gcgaggaaga gaaatggttc aacagcctgc cggagggcct gcgtgagcgt      420

ctgaccgaac tggagggtag cagcgaagag aacctgtatt ttcaaggcct cgagcaccac      480

caccaccacc ac                                                          492


<210>  54
<211>  495
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cis-CaaD from Coryneform sp. codon-optimized for Escherichia coli
       plus TEV protease recognition site and C-terminal His6-tag

<400>  54
atgccggttt acatggttta cgttagccag gaccgtctga ccccgagcgc gaagcacgcg       60

gttgcgaagg cgattaccga tgcgcaccgt ggtctgaccg gcacccagca cttcctggcg      120

caagtgaact ttcaggagca accggcgggt aacgtgttcc tgggtggcgt tcagcaaggt      180

ggcgacacca tctttgttca tggtctgcac cgtgagggcc gtagcgcgga tctgaagggc      240

cagctggcgc aacgtattgt tgacgatgtg agcgttgcgg cggaaatcga ccgtaaacac      300

atttgggtgt acttcggcga gatgccggcg cagcaaatgg ttgaatatgg ccgtttcctg      360

ccgcagccgg gtcatgaggg tgaatggttt gacaacctga gcagcgatga acgtgcgttt      420

atggagacca atgttgatgt gagccgtacc gagaacctgt attttcaagg cctcgagcac      480

caccaccacc accac                                                       495


<210>  55
<211>  1068
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered phosphite dehydrogenase (PTDH) from Pseudomonas 
       stutzeri plus N-terminal His6-tag from pET-15b vector

<400>  55
atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat       60

atgctgccga aactcgttat aactcaccga gtacacgaag agatcctgca actgctggcg      120

ccacattgcg agctgataac caaccagacc gacagcacgc tgacgcgcga ggaaattctg      180

cgccgctgtc gcgatgctca ggcgatgatg gcgttcatgc ccgatcgggt cgatgcagac      240

tttcttcaag cctgccctga gctgcgtgta atcggctgcg cgctcaaggg cttcgacaat      300

ttcgatgtgg acgcctgtac tgcccgcggg gtctggctga ccttcgtgcc tgatctgttg      360

acggtcccga ctgccgagct ggcgatcgga ctggcggtgg ggctggggcg gcatctgcgg      420

gcagcagatg cgttcgtccg ctctggcaag ttccggggct ggcaaccacg gttctacggc      480

acggggctgg ataacgctac ggtcggcttc cttggcatgg gcgccatcgg actggccatg      540

gctgatcgct tgcagggatg gggcgcgacc ctgcagtacc acgcggcgaa ggctctggat      600

acacaaaccg agcaacggct cggcctgcgc caggtggcgt gcagcgaact cttcgccagc      660

tcggacttca tcctgctggc gcttcccttg aatgccgata ccctgcatct ggtcaacgcc      720

gagctgcttg ccctcgtacg gccgggcgct ctgcttgtaa acccctgtcg tggctcggta      780

gtggatgaag ccgccgtgct cgcggcgctt gagcgaggcc agctcggcgg gtatgcggcg      840

gatgtattcg aaatggaaga ctgggctcgc gcggaccggc cgcagcagat cgatcctgcg      900

ctgctcgcgc atccgaatac gctgttcact ccgcacatag ggtcggcagt gcgcgcggtg      960

cgcctggaga ttgaacgttg tgcagcgcag aacatcctcc aggcattggc aggtgagcgc     1020

ccaatcaacg ctgtgaaccg tctgcccaag gccaatcctg ccgcagac                  1068


<210>  56
<211>  456
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Malonate semialdehyde decarboxylase (MSAD) from Coryneform sp. 
       FG41 (NCBI: MZ369160) plus TEV protease recognition site and 
       C-terminal His6-tag

<400>  56
atgcccttaa tccgtataga tcttaccagt gatcgttcga gagagcaacg gcgggcgatt       60

gctgatgcag tccatgacgc tttagtagaa gttttagcga ttccggctcg tgatcgcttc      120

cagatactga ctgcgcacga tccctctgat attatagccg aagatgctgg acttggcttt      180

cagcggtccc ccagtgtagt catcatacac gtctttacac aggcaggtag aactattgaa      240

acgaaacaga gagtatttgc agcgataaca gaaagtctgg ctccaatcgg tgttgcagga      300

tctgatgttt ttatcgcaat caccgaaaat gcaccccatg actggagctt tgggtttggc      360

agtgcacaat atgtcacggg tgaacttgcg attccagcca ctggtgcggc tgagaacctg      420

tattttcaag gcctcgagca ccaccaccac caccac                                456


<210>  57
<211>  789
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YdfG from Escherichia coli plus TEV protease recognition site and
       C-terminal His6-tag

<400>  57
atgatcgttt tagtaactgg agcaacggca ggttttggtg aatgcattac tcgtcgtttt       60

attcaacaag ggcataaagt tatcgccact ggccgtcgcc aggaacggtt gcaggagtta      120

aaagacgaac tgggagataa tctgtatatc gcccaactgg acgttcgcaa ccgcgccgct      180

attgaagaga tgctggcatc gcttcctgcc gagtggtgca atattgatat cctggtaaat      240

aatgccggcc tggcgttggg catggagcct gcgcataaag ccagcgttga agactgggaa      300

acgatgattg ataccaacaa caaaggcctg gtatatatga cgcgcgccgt cttaccgggt      360

atggttgaac gtaatcatgg tcatattatt aacattggct caacggcagg tagctggccg      420

tatgccggtg gtaacgttta cggtgcgacg aaagcgtttg ttcgtcagtt tagcctgaat      480

ctgcgtacgg atctgcatgg tacggcggtg cgcgtcaccg acatcgaacc gggtctggtg      540

ggtggtaccg agttttccaa tgtccgcttt aaaggcgatg acggtaaagc agaaaaaacc      600

tatcaaaata ccgttgcatt gacgccagaa gatgtcagcg aagccgtctg gtgggtgtca      660

acgctgcctg ctcacgtcaa tatcaatacc ctggaaatga tgccggttac ccaaagctat      720

gccggactga atgtccaccg tcaggagaac ctgtattttc aaggcctcga gcaccaccac      780

caccaccac                                                              789


<210>  58
<211>  930
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MmsB from Pseudomonas putida KT2440 plus TEV protease recognition
       site and C-terminal His6-tag

<400>  58
atgcgtatcg cattcatcgg cctgggcaac atgggcgcgc ccatggcccg caacctgatc       60

aaggccgggc atcagctgaa cctgttcgac ctgaacaagg ccgtgctggc cgagctggca      120

gaactgggcg ggcagatcag cccctcgccc aaggacgcgg cggccaacag cgagctggtg      180

atcaccatgc tgccggccgc agcccatgtg cgtagcgtgt acttgaacga ggacggcgta      240

ctggccggta ttcgtcctgg cacgccgacc gttgactgca gcaccatcga cccgcagacc      300

gcacgtgacg tgtccaaggc cgcagcggca aagggcgtgg acatggggga tgcgccggtt      360

tccggtggta ctggcggcgc ggcggccggc accctgacgt tcatggtcgg cgccagtacc      420

gagttgttcg ccagcctcaa gccggtactg gagcagatgg gccgcaacat cgtgcactgc      480

ggggaagtcg gtaccggcca gatcgccaag atctgcaaca acctgctgct cggcatttcg      540

atgatcggcg tgtccgaggc catggccctg ggtaacgcgc tgggtatcga taccaaggtg      600

ctggccggca tcatcaacag ttcgaccggg cgttgctgga gctcggacac ctacaacccg      660

tggccgggca tcatcgaaac cgcacctgca tcgcgtggct acaccggtgg ctttggcgcc      720

gaactcatgc tcaaggacct ggggttggcc accgaagcgg cacgccaggc acaccaaccg      780

gtgattctcg gtgccgtggc ccagcagctg taccaggcca tgagcctgcg aggcgagggt      840

ggcaaggact tctcggccat cgtcgagggt tatcgcaaga aggatgagaa cctgtatttt      900

caaggcctcg agcaccacca ccaccaccac                                       930


<210>  59
<211>  163
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cg10062 from Corynebacterium glutamicum codon-optimized for 
       Escherichia coli plus TEV protease recognition site and 
       C-terminal His6-tag

<400>  59

Pro Thr Tyr Thr Cys Trp Ser Gln Arg Ile Arg Ile Ser Arg Glu Ala 
1               5                   10                  15      


Lys Gln Arg Ile Ala Glu Ala Ile Thr Asp Ala His His Glu Leu Ala 
            20                  25                  30          


His Ala Pro Lys Tyr Leu Val Gln Val Ile Phe Asn Glu Val Glu Pro 
        35                  40                  45              


Asp Ser Tyr Phe Ile Ala Ala Gln Ser Ala Ser Glu Asn His Ile Trp 
    50                  55                  60                  


Val Gln Ala Thr Ile Arg Ser Gly Arg Thr Glu Lys Gln Lys Glu Glu 
65                  70                  75                  80  


Leu Leu Leu Arg Leu Thr Gln Glu Ile Ala Leu Ile Leu Gly Ile Pro 
                85                  90                  95      


Asn Glu Glu Val Trp Val Tyr Ile Thr Glu Ile Pro Gly Ser Asn Met 
            100                 105                 110         


Thr Glu Tyr Gly Arg Leu Leu Met Glu Pro Gly Glu Glu Glu Lys Trp 
        115                 120                 125             


Phe Asn Ser Leu Pro Glu Gly Leu Arg Glu Arg Leu Thr Glu Leu Glu 
    130                 135                 140                 


Gly Ser Ser Glu Glu Asn Leu Tyr Phe Gln Gly Leu Glu His His His 
145                 150                 155                 160 


His His His 
            


<210>  60
<211>  163
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cg10062(E114Q) from Corynebacterium glutamicum codon-optimized 
       for Escherichia coli plus TEV protease recognition site and 
       C-terminal His6-tag

<400>  60

Pro Thr Tyr Thr Cys Trp Ser Gln Arg Ile Arg Ile Ser Arg Glu Ala 
1               5                   10                  15      


Lys Gln Arg Ile Ala Glu Ala Ile Thr Asp Ala His His Glu Leu Ala 
            20                  25                  30          


His Ala Pro Lys Tyr Leu Val Gln Val Ile Phe Asn Glu Val Glu Pro 
        35                  40                  45              


Asp Ser Tyr Phe Ile Ala Ala Gln Ser Ala Ser Glu Asn His Ile Trp 
    50                  55                  60                  


Val Gln Ala Thr Ile Arg Ser Gly Arg Thr Glu Lys Gln Lys Glu Glu 
65                  70                  75                  80  


Leu Leu Leu Arg Leu Thr Gln Glu Ile Ala Leu Ile Leu Gly Ile Pro 
                85                  90                  95      


Asn Glu Glu Val Trp Val Tyr Ile Thr Glu Ile Pro Gly Ser Asn Met 
            100                 105                 110         


Thr Gln Tyr Gly Arg Leu Leu Met Glu Pro Gly Glu Glu Glu Lys Trp 
        115                 120                 125             


Phe Asn Ser Leu Pro Glu Gly Leu Arg Glu Arg Leu Thr Glu Leu Glu 
    130                 135                 140                 


Gly Ser Ser Glu Glu Asn Leu Tyr Phe Gln Gly Leu Glu His His His 
145                 150                 155                 160 


His His His 
            


<210>  61
<211>  163
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cg10062(E114D) from Corynebacterium glutamicum codon-optimized 
       for Escherichia coli plus TEV protease recognition site and 
       C-terminal His6-tag

<400>  61

Pro Thr Tyr Thr Cys Trp Ser Gln Arg Ile Arg Ile Ser Arg Glu Ala 
1               5                   10                  15      


Lys Gln Arg Ile Ala Glu Ala Ile Thr Asp Ala His His Glu Leu Ala 
            20                  25                  30          


His Ala Pro Lys Tyr Leu Val Gln Val Ile Phe Asn Glu Val Glu Pro 
        35                  40                  45              


Asp Ser Tyr Phe Ile Ala Ala Gln Ser Ala Ser Glu Asn His Ile Trp 
    50                  55                  60                  


Val Gln Ala Thr Ile Arg Ser Gly Arg Thr Glu Lys Gln Lys Glu Glu 
65                  70                  75                  80  


Leu Leu Leu Arg Leu Thr Gln Glu Ile Ala Leu Ile Leu Gly Ile Pro 
                85                  90                  95      


Asn Glu Glu Val Trp Val Tyr Ile Thr Glu Ile Pro Gly Ser Asn Met 
            100                 105                 110         


Thr Asp Tyr Gly Arg Leu Leu Met Glu Pro Gly Glu Glu Glu Lys Trp 
        115                 120                 125             


Phe Asn Ser Leu Pro Glu Gly Leu Arg Glu Arg Leu Thr Glu Leu Glu 
    130                 135                 140                 


Gly Ser Ser Glu Glu Asn Leu Tyr Phe Gln Gly Leu Glu His His His 
145                 150                 155                 160 


His His His 
            


<210>  62
<211>  163
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cg10062(E114N) from Corynebacterium glutamicum codon-optimized 
       for Escherichia coli plus TEV protease recognition site and 
       C-terminal His6-tag

<400>  62

Pro Thr Tyr Thr Cys Trp Ser Gln Arg Ile Arg Ile Ser Arg Glu Ala 
1               5                   10                  15      


Lys Gln Arg Ile Ala Glu Ala Ile Thr Asp Ala His His Glu Leu Ala 
            20                  25                  30          


His Ala Pro Lys Tyr Leu Val Gln Val Ile Phe Asn Glu Val Glu Pro 
        35                  40                  45              


Asp Ser Tyr Phe Ile Ala Ala Gln Ser Ala Ser Glu Asn His Ile Trp 
    50                  55                  60                  


Val Gln Ala Thr Ile Arg Ser Gly Arg Thr Glu Lys Gln Lys Glu Glu 
65                  70                  75                  80  


Leu Leu Leu Arg Leu Thr Gln Glu Ile Ala Leu Ile Leu Gly Ile Pro 
                85                  90                  95      


Asn Glu Glu Val Trp Val Tyr Ile Thr Glu Ile Pro Gly Ser Asn Met 
            100                 105                 110         


Thr Asn Tyr Gly Arg Leu Leu Met Glu Pro Gly Glu Glu Glu Lys Trp 
        115                 120                 125             


Phe Asn Ser Leu Pro Glu Gly Leu Arg Glu Arg Leu Thr Glu Leu Glu 
    130                 135                 140                 


Gly Ser Ser Glu Glu Asn Leu Tyr Phe Gln Gly Leu Glu His His His 
145                 150                 155                 160 


His His His 
            


<210>  63
<211>  163
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cg10062(H28A) from Corynebacterium glutamicum codon-optimized for
       Escherichia coli plus TEV protease recognition site and 
       C-terminal His6-tag

<400>  63

Pro Thr Tyr Thr Cys Trp Ser Gln Arg Ile Arg Ile Ser Arg Glu Ala 
1               5                   10                  15      


Lys Gln Arg Ile Ala Glu Ala Ile Thr Asp Ala Ala His Glu Leu Ala 
            20                  25                  30          


His Ala Pro Lys Tyr Leu Val Gln Val Ile Phe Asn Glu Val Glu Pro 
        35                  40                  45              


Asp Ser Tyr Phe Ile Ala Ala Gln Ser Ala Ser Glu Asn His Ile Trp 
    50                  55                  60                  


Val Gln Ala Thr Ile Arg Ser Gly Arg Thr Glu Lys Gln Lys Glu Glu 
65                  70                  75                  80  


Leu Leu Leu Arg Leu Thr Gln Glu Ile Ala Leu Ile Leu Gly Ile Pro 
                85                  90                  95      


Asn Glu Glu Val Trp Val Tyr Ile Thr Glu Ile Pro Gly Ser Asn Met 
            100                 105                 110         


Thr Glu Tyr Gly Arg Leu Leu Met Glu Pro Gly Glu Glu Glu Lys Trp 
        115                 120                 125             


Phe Asn Ser Leu Pro Glu Gly Leu Arg Glu Arg Leu Thr Glu Leu Glu 
    130                 135                 140                 


Gly Ser Ser Glu Glu Asn Leu Tyr Phe Gln Gly Leu Glu His His His 
145                 150                 155                 160 


His His His 
            


<210>  64
<211>  163
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cg10062(R70A) from Corynebacterium glutamicum codon-optimized for
       Escherichia coli plus TEV protease recognition site and 
       C-terminal His6-tag

<400>  64

Pro Thr Tyr Thr Cys Trp Ser Gln Arg Ile Arg Ile Ser Arg Glu Ala 
1               5                   10                  15      


Lys Gln Arg Ile Ala Glu Ala Ile Thr Asp Ala His His Glu Leu Ala 
            20                  25                  30          


His Ala Pro Lys Tyr Leu Val Gln Val Ile Phe Asn Glu Val Glu Pro 
        35                  40                  45              


Asp Ser Tyr Phe Ile Ala Ala Gln Ser Ala Ser Glu Asn His Ile Trp 
    50                  55                  60                  


Val Gln Ala Thr Ile Ala Ser Gly Arg Thr Glu Lys Gln Lys Glu Glu 
65                  70                  75                  80  


Leu Leu Leu Arg Leu Thr Gln Glu Ile Ala Leu Ile Leu Gly Ile Pro 
                85                  90                  95      


Asn Glu Glu Val Trp Val Tyr Ile Thr Glu Ile Pro Gly Ser Asn Met 
            100                 105                 110         


Thr Glu Tyr Gly Arg Leu Leu Met Glu Pro Gly Glu Glu Glu Lys Trp 
        115                 120                 125             


Phe Asn Ser Leu Pro Glu Gly Leu Arg Glu Arg Leu Thr Glu Leu Glu 
    130                 135                 140                 


Gly Ser Ser Glu Glu Asn Leu Tyr Phe Gln Gly Leu Glu His His His 
145                 150                 155                 160 


His His His 
            


<210>  65
<211>  163
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cg10062(R70K) from Corynebacterium glutamicum codon-optimized for
       Escherichia coli plus TEV protease recognition site and 
       C-terminal His6-tag

<400>  65

Pro Thr Tyr Thr Cys Trp Ser Gln Arg Ile Arg Ile Ser Arg Glu Ala 
1               5                   10                  15      


Lys Gln Arg Ile Ala Glu Ala Ile Thr Asp Ala His His Glu Leu Ala 
            20                  25                  30          


His Ala Pro Lys Tyr Leu Val Gln Val Ile Phe Asn Glu Val Glu Pro 
        35                  40                  45              


Asp Ser Tyr Phe Ile Ala Ala Gln Ser Ala Ser Glu Asn His Ile Trp 
    50                  55                  60                  


Val Gln Ala Thr Ile Lys Ser Gly Arg Thr Glu Lys Gln Lys Glu Glu 
65                  70                  75                  80  


Leu Leu Leu Arg Leu Thr Gln Glu Ile Ala Leu Ile Leu Gly Ile Pro 
                85                  90                  95      


Asn Glu Glu Val Trp Val Tyr Ile Thr Glu Ile Pro Gly Ser Asn Met 
            100                 105                 110         


Thr Glu Tyr Gly Arg Leu Leu Met Glu Pro Gly Glu Glu Glu Lys Trp 
        115                 120                 125             


Phe Asn Ser Leu Pro Glu Gly Leu Arg Glu Arg Leu Thr Glu Leu Glu 
    130                 135                 140                 


Gly Ser Ser Glu Glu Asn Leu Tyr Phe Gln Gly Leu Glu His His His 
145                 150                 155                 160 


His His His 
            


<210>  66
<211>  163
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cg10062(R73A) from Corynebacterium glutamicum codon-optimized for
       Escherichia coli plus TEV protease recognition site and 
       C-terminal His6-tag

<400>  66

Pro Thr Tyr Thr Cys Trp Ser Gln Arg Ile Arg Ile Ser Arg Glu Ala 
1               5                   10                  15      


Lys Gln Arg Ile Ala Glu Ala Ile Thr Asp Ala His His Glu Leu Ala 
            20                  25                  30          


His Ala Pro Lys Tyr Leu Val Gln Val Ile Phe Asn Glu Val Glu Pro 
        35                  40                  45              


Asp Ser Tyr Phe Ile Ala Ala Gln Ser Ala Ser Glu Asn His Ile Trp 
    50                  55                  60                  


Val Gln Ala Thr Ile Arg Ser Gly Ala Thr Glu Lys Gln Lys Glu Glu 
65                  70                  75                  80  


Leu Leu Leu Arg Leu Thr Gln Glu Ile Ala Leu Ile Leu Gly Ile Pro 
                85                  90                  95      


Asn Glu Glu Val Trp Val Tyr Ile Thr Glu Ile Pro Gly Ser Asn Met 
            100                 105                 110         


Thr Glu Tyr Gly Arg Leu Leu Met Glu Pro Gly Glu Glu Glu Lys Trp 
        115                 120                 125             


Phe Asn Ser Leu Pro Glu Gly Leu Arg Glu Arg Leu Thr Glu Leu Glu 
    130                 135                 140                 


Gly Ser Ser Glu Glu Asn Leu Tyr Phe Gln Gly Leu Glu His His His 
145                 150                 155                 160 


His His His 
            


<210>  67
<211>  163
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cg10062(R73K) from Corynebacterium glutamicum codon-optimized for
       Escherichia coli plus TEV protease recognition site and 
       C-terminal His6-tag

<400>  67

Pro Thr Tyr Thr Cys Trp Ser Gln Arg Ile Arg Ile Ser Arg Glu Ala 
1               5                   10                  15      


Lys Gln Arg Ile Ala Glu Ala Ile Thr Asp Ala His His Glu Leu Ala 
            20                  25                  30          


His Ala Pro Lys Tyr Leu Val Gln Val Ile Phe Asn Glu Val Glu Pro 
        35                  40                  45              


Asp Ser Tyr Phe Ile Ala Ala Gln Ser Ala Ser Glu Asn His Ile Trp 
    50                  55                  60                  


Val Gln Ala Thr Ile Arg Ser Gly Lys Thr Glu Lys Gln Lys Glu Glu 
65                  70                  75                  80  


Leu Leu Leu Arg Leu Thr Gln Glu Ile Ala Leu Ile Leu Gly Ile Pro 
                85                  90                  95      


Asn Glu Glu Val Trp Val Tyr Ile Thr Glu Ile Pro Gly Ser Asn Met 
            100                 105                 110         


Thr Glu Tyr Gly Arg Leu Leu Met Glu Pro Gly Glu Glu Glu Lys Trp 
        115                 120                 125             


Phe Asn Ser Leu Pro Glu Gly Leu Arg Glu Arg Leu Thr Glu Leu Glu 
    130                 135                 140                 


Gly Ser Ser Glu Glu Asn Leu Tyr Phe Gln Gly Leu Glu His His His 
145                 150                 155                 160 


His His His 
            


<210>  68
<211>  163
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cg10062(Y103A) from Corynebacterium glutamicum codon-optimized 
       for Escherichia coli plus TEV protease recognition site and 
       C-terminal His6-tag

<400>  68

Pro Thr Tyr Thr Cys Trp Ser Gln Arg Ile Arg Ile Ser Arg Glu Ala 
1               5                   10                  15      


Lys Gln Arg Ile Ala Glu Ala Ile Thr Asp Ala His His Glu Leu Ala 
            20                  25                  30          


His Ala Pro Lys Tyr Leu Val Gln Val Ile Phe Asn Glu Val Glu Pro 
        35                  40                  45              


Asp Ser Tyr Phe Ile Ala Ala Gln Ser Ala Ser Glu Asn His Ile Trp 
    50                  55                  60                  


Val Gln Ala Thr Ile Arg Ser Gly Arg Thr Glu Lys Gln Lys Glu Glu 
65                  70                  75                  80  


Leu Leu Leu Arg Leu Thr Gln Glu Ile Ala Leu Ile Leu Gly Ile Pro 
                85                  90                  95      


Asn Glu Glu Val Trp Val Ala Ile Thr Glu Ile Pro Gly Ser Asn Met 
            100                 105                 110         


Thr Glu Tyr Gly Arg Leu Leu Met Glu Pro Gly Glu Glu Glu Lys Trp 
        115                 120                 125             


Phe Asn Ser Leu Pro Glu Gly Leu Arg Glu Arg Leu Thr Glu Leu Glu 
    130                 135                 140                 


Gly Ser Ser Glu Glu Asn Leu Tyr Phe Gln Gly Leu Glu His His His 
145                 150                 155                 160 


His His His 
            


<210>  69
<211>  163
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cg10062(Y103F) from Corynebacterium glutamicum codon-optimized 
       for Escherichia coli plus TEV protease recognition site and 
       C-terminal His6-tag

<400>  69

Pro Thr Tyr Thr Cys Trp Ser Gln Arg Ile Arg Ile Ser Arg Glu Ala 
1               5                   10                  15      


Lys Gln Arg Ile Ala Glu Ala Ile Thr Asp Ala His His Glu Leu Ala 
            20                  25                  30          


His Ala Pro Lys Tyr Leu Val Gln Val Ile Phe Asn Glu Val Glu Pro 
        35                  40                  45              


Asp Ser Tyr Phe Ile Ala Ala Gln Ser Ala Ser Glu Asn His Ile Trp 
    50                  55                  60                  


Val Gln Ala Thr Ile Arg Ser Gly Arg Thr Glu Lys Gln Lys Glu Glu 
65                  70                  75                  80  


Leu Leu Leu Arg Leu Thr Gln Glu Ile Ala Leu Ile Leu Gly Ile Pro 
                85                  90                  95      


Asn Glu Glu Val Trp Val Phe Ile Thr Glu Ile Pro Gly Ser Asn Met 
            100                 105                 110         


Thr Glu Tyr Gly Arg Leu Leu Met Glu Pro Gly Glu Glu Glu Lys Trp 
        115                 120                 125             


Phe Asn Ser Leu Pro Glu Gly Leu Arg Glu Arg Leu Thr Glu Leu Glu 
    130                 135                 140                 


Gly Ser Ser Glu Glu Asn Leu Tyr Phe Gln Gly Leu Glu His His His 
145                 150                 155                 160 


His His His 
            


<210>  70
<211>  163
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cg10062(E114A) from Corynebacterium glutamicum codon-optimized 
       for Escherichia coli plus TEV protease recognition site and 
       C-terminal His6-tag

<400>  70

Pro Thr Tyr Thr Cys Trp Ser Gln Arg Ile Arg Ile Ser Arg Glu Ala 
1               5                   10                  15      


Lys Gln Arg Ile Ala Glu Ala Ile Thr Asp Ala His His Glu Leu Ala 
            20                  25                  30          


His Ala Pro Lys Tyr Leu Val Gln Val Ile Phe Asn Glu Val Glu Pro 
        35                  40                  45              


Asp Ser Tyr Phe Ile Ala Ala Gln Ser Ala Ser Glu Asn His Ile Trp 
    50                  55                  60                  


Val Gln Ala Thr Ile Arg Ser Gly Arg Thr Glu Lys Gln Lys Glu Glu 
65                  70                  75                  80  


Leu Leu Leu Arg Leu Thr Gln Glu Ile Ala Leu Ile Leu Gly Ile Pro 
                85                  90                  95      


Asn Glu Glu Val Trp Val Tyr Ile Thr Glu Ile Pro Gly Ser Asn Met 
            100                 105                 110         


Thr Ala Tyr Gly Arg Leu Leu Met Glu Pro Gly Glu Glu Glu Lys Trp 
        115                 120                 125             


Phe Asn Ser Leu Pro Glu Gly Leu Arg Glu Arg Leu Thr Glu Leu Glu 
    130                 135                 140                 


Gly Ser Ser Glu Glu Asn Leu Tyr Phe Gln Gly Leu Glu His His His 
145                 150                 155                 160 


His His His 
            


<210>  71
<211>  163
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cg10062(E114D-Y103F) from Corynebacterium glutamicum 
       codon-optimized for Escherichia coli plus TEV protease 
       recognition site and C-terminal His6-tag

<400>  71

Pro Thr Tyr Thr Cys Trp Ser Gln Arg Ile Arg Ile Ser Arg Glu Ala 
1               5                   10                  15      


Lys Gln Arg Ile Ala Glu Ala Ile Thr Asp Ala His His Glu Leu Ala 
            20                  25                  30          


His Ala Pro Lys Tyr Leu Val Gln Val Ile Phe Asn Glu Val Glu Pro 
        35                  40                  45              


Asp Ser Tyr Phe Ile Ala Ala Gln Ser Ala Ser Glu Asn His Ile Trp 
    50                  55                  60                  


Val Gln Ala Thr Ile Arg Ser Gly Arg Thr Glu Lys Gln Lys Glu Glu 
65                  70                  75                  80  


Leu Leu Leu Arg Leu Thr Gln Glu Ile Ala Leu Ile Leu Gly Ile Pro 
                85                  90                  95      


Asn Glu Glu Val Trp Val Phe Ile Thr Glu Ile Pro Gly Ser Asn Met 
            100                 105                 110         


Thr Asp Tyr Gly Arg Leu Leu Met Glu Pro Gly Glu Glu Glu Lys Trp 
        115                 120                 125             


Phe Asn Ser Leu Pro Glu Gly Leu Arg Glu Arg Leu Thr Glu Leu Glu 
    130                 135                 140                 


Gly Ser Ser Glu Glu Asn Leu Tyr Phe Gln Gly Leu Glu His His His 
145                 150                 155                 160 


His His His 
            


<210>  72
<211>  164
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cis-CaaD from Coryneform sp. codon-optimized for Escherichia coli
       plus TEV protease recognition site and C-terminal His6-tag

<400>  72

Pro Val Tyr Met Val Tyr Val Ser Gln Asp Arg Leu Thr Pro Ser Ala 
1               5                   10                  15      


Lys His Ala Val Ala Lys Ala Ile Thr Asp Ala His Arg Gly Leu Thr 
            20                  25                  30          


Gly Thr Gln His Phe Leu Ala Gln Val Asn Phe Gln Glu Gln Pro Ala 
        35                  40                  45              


Gly Asn Val Phe Leu Gly Gly Val Gln Gln Gly Gly Asp Thr Ile Phe 
    50                  55                  60                  


Val His Gly Leu His Arg Glu Gly Arg Ser Ala Asp Leu Lys Gly Gln 
65                  70                  75                  80  


Leu Ala Gln Arg Ile Val Asp Asp Val Ser Val Ala Ala Glu Ile Asp 
                85                  90                  95      


Arg Lys His Ile Trp Val Tyr Phe Gly Glu Met Pro Ala Gln Gln Met 
            100                 105                 110         


Val Glu Tyr Gly Arg Phe Leu Pro Gln Pro Gly His Glu Gly Glu Trp 
        115                 120                 125             


Phe Asp Asn Leu Ser Ser Asp Glu Arg Ala Phe Met Glu Thr Asn Val 
    130                 135                 140                 


Asp Val Ser Arg Thr Glu Asn Leu Tyr Phe Gln Gly Leu Glu His His 
145                 150                 155                 160 


His His His His 
                


<210>  73
<211>  356
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered phosphite dehydrogenase (PTDH) from Pseudomonas 
       stutzeri plus N-terminal His6-tag from pET-15b vector

<400>  73

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Leu Pro Lys Leu Val Ile Thr His Arg Val His 
            20                  25                  30          


Glu Glu Ile Leu Gln Leu Leu Ala Pro His Cys Glu Leu Ile Thr Asn 
        35                  40                  45              


Gln Thr Asp Ser Thr Leu Thr Arg Glu Glu Ile Leu Arg Arg Cys Arg 
    50                  55                  60                  


Asp Ala Gln Ala Met Met Ala Phe Met Pro Asp Arg Val Asp Ala Asp 
65                  70                  75                  80  


Phe Leu Gln Ala Cys Pro Glu Leu Arg Val Ile Gly Cys Ala Leu Lys 
                85                  90                  95      


Gly Phe Asp Asn Phe Asp Val Asp Ala Cys Thr Ala Arg Gly Val Trp 
            100                 105                 110         


Leu Thr Phe Val Pro Asp Leu Leu Thr Val Pro Thr Ala Glu Leu Ala 
        115                 120                 125             


Ile Gly Leu Ala Val Gly Leu Gly Arg His Leu Arg Ala Ala Asp Ala 
    130                 135                 140                 


Phe Val Arg Ser Gly Lys Phe Arg Gly Trp Gln Pro Arg Phe Tyr Gly 
145                 150                 155                 160 


Thr Gly Leu Asp Asn Ala Thr Val Gly Phe Leu Gly Met Gly Ala Ile 
                165                 170                 175     


Gly Leu Ala Met Ala Asp Arg Leu Gln Gly Trp Gly Ala Thr Leu Gln 
            180                 185                 190         


Tyr His Ala Ala Lys Ala Leu Asp Thr Gln Thr Glu Gln Arg Leu Gly 
        195                 200                 205             


Leu Arg Gln Val Ala Cys Ser Glu Leu Phe Ala Ser Ser Asp Phe Ile 
    210                 215                 220                 


Leu Leu Ala Leu Pro Leu Asn Ala Asp Thr Leu His Leu Val Asn Ala 
225                 230                 235                 240 


Glu Leu Leu Ala Leu Val Arg Pro Gly Ala Leu Leu Val Asn Pro Cys 
                245                 250                 255     


Arg Gly Ser Val Val Asp Glu Ala Ala Val Leu Ala Ala Leu Glu Arg 
            260                 265                 270         


Gly Gln Leu Gly Gly Tyr Ala Ala Asp Val Phe Glu Met Glu Asp Trp 
        275                 280                 285             


Ala Arg Ala Asp Arg Pro Gln Gln Ile Asp Pro Ala Leu Leu Ala His 
    290                 295                 300                 


Pro Asn Thr Leu Phe Thr Pro His Ile Gly Ser Ala Val Arg Ala Val 
305                 310                 315                 320 


Arg Leu Glu Ile Glu Arg Cys Ala Ala Gln Asn Ile Leu Gln Ala Leu 
                325                 330                 335     


Ala Gly Glu Arg Pro Ile Asn Ala Val Asn Arg Leu Pro Lys Ala Asn 
            340                 345                 350         


Pro Ala Ala Asp 
        355     


<210>  74
<211>  151
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Malonate semialdehyde decarboxylase (MSAD) from Coryneform sp. 
       FG41 plus TEV protease recognition site and C-terminal His6-tag

<400>  74

Pro Leu Ile Arg Ile Asp Leu Thr Ser Asp Arg Ser Arg Glu Gln Arg 
1               5                   10                  15      


Arg Ala Ile Ala Asp Ala Val His Asp Ala Leu Val Glu Val Leu Ala 
            20                  25                  30          


Ile Pro Ala Arg Asp Arg Phe Gln Ile Leu Thr Ala His Asp Pro Ser 
        35                  40                  45              


Asp Ile Ile Ala Glu Asp Ala Gly Leu Gly Phe Gln Arg Ser Pro Ser 
    50                  55                  60                  


Val Val Ile Ile His Val Phe Thr Gln Ala Gly Arg Thr Ile Glu Thr 
65                  70                  75                  80  


Lys Gln Arg Val Phe Ala Ala Ile Thr Glu Ser Leu Ala Pro Ile Gly 
                85                  90                  95      


Val Ala Gly Ser Asp Val Phe Ile Ala Ile Thr Glu Asn Ala Pro His 
            100                 105                 110         


Asp Trp Ser Phe Gly Phe Gly Ser Ala Gln Tyr Val Thr Gly Glu Leu 
        115                 120                 125             


Ala Ile Pro Ala Thr Gly Ala Ala Glu Asn Leu Tyr Phe Gln Gly Leu 
    130                 135                 140                 


Glu His His His His His His 
145                 150     


<210>  75
<211>  263
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  YdfG from Escherichia coli plus TEV protease recognition site and
       C-terminal His6-tag

<400>  75

Met Ile Val Leu Val Thr Gly Ala Thr Ala Gly Phe Gly Glu Cys Ile 
1               5                   10                  15      


Thr Arg Arg Phe Ile Gln Gln Gly His Lys Val Ile Ala Thr Gly Arg 
            20                  25                  30          


Arg Gln Glu Arg Leu Gln Glu Leu Lys Asp Glu Leu Gly Asp Asn Leu 
        35                  40                  45              


Tyr Ile Ala Gln Leu Asp Val Arg Asn Arg Ala Ala Ile Glu Glu Met 
    50                  55                  60                  


Leu Ala Ser Leu Pro Ala Glu Trp Cys Asn Ile Asp Ile Leu Val Asn 
65                  70                  75                  80  


Asn Ala Gly Leu Ala Leu Gly Met Glu Pro Ala His Lys Ala Ser Val 
                85                  90                  95      


Glu Asp Trp Glu Thr Met Ile Asp Thr Asn Asn Lys Gly Leu Val Tyr 
            100                 105                 110         


Met Thr Arg Ala Val Leu Pro Gly Met Val Glu Arg Asn His Gly His 
        115                 120                 125             


Ile Ile Asn Ile Gly Ser Thr Ala Gly Ser Trp Pro Tyr Ala Gly Gly 
    130                 135                 140                 


Asn Val Tyr Gly Ala Thr Lys Ala Phe Val Arg Gln Phe Ser Leu Asn 
145                 150                 155                 160 


Leu Arg Thr Asp Leu His Gly Thr Ala Val Arg Val Thr Asp Ile Glu 
                165                 170                 175     


Pro Gly Leu Val Gly Gly Thr Glu Phe Ser Asn Val Arg Phe Lys Gly 
            180                 185                 190         


Asp Asp Gly Lys Ala Glu Lys Thr Tyr Gln Asn Thr Val Ala Leu Thr 
        195                 200                 205             


Pro Glu Asp Val Ser Glu Ala Val Trp Trp Val Ser Thr Leu Pro Ala 
    210                 215                 220                 


His Val Asn Ile Asn Thr Leu Glu Met Met Pro Val Thr Gln Ser Tyr 
225                 230                 235                 240 


Ala Gly Leu Asn Val His Arg Gln Glu Asn Leu Tyr Phe Gln Gly Leu 
                245                 250                 255     


Glu His His His His His His 
            260             


<210>  76
<211>  310
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MmsB from Pseudomonas putida KT2440 plus TEV protease recognition
       site and C-terminal His6-tag

<400>  76

Met Arg Ile Ala Phe Ile Gly Leu Gly Asn Met Gly Ala Pro Met Ala 
1               5                   10                  15      


Arg Asn Leu Ile Lys Ala Gly His Gln Leu Asn Leu Phe Asp Leu Asn 
            20                  25                  30          


Lys Ala Val Leu Ala Glu Leu Ala Glu Leu Gly Gly Gln Ile Ser Pro 
        35                  40                  45              


Ser Pro Lys Asp Ala Ala Ala Asn Ser Glu Leu Val Ile Thr Met Leu 
    50                  55                  60                  


Pro Ala Ala Ala His Val Arg Ser Val Tyr Leu Asn Glu Asp Gly Val 
65                  70                  75                  80  


Leu Ala Gly Ile Arg Pro Gly Thr Pro Thr Val Asp Cys Ser Thr Ile 
                85                  90                  95      


Asp Pro Gln Thr Ala Arg Asp Val Ser Lys Ala Ala Ala Ala Lys Gly 
            100                 105                 110         


Val Asp Met Gly Asp Ala Pro Val Ser Gly Gly Thr Gly Gly Ala Ala 
        115                 120                 125             


Ala Gly Thr Leu Thr Phe Met Val Gly Ala Ser Thr Glu Leu Phe Ala 
    130                 135                 140                 


Ser Leu Lys Pro Val Leu Glu Gln Met Gly Arg Asn Ile Val His Cys 
145                 150                 155                 160 


Gly Glu Val Gly Thr Gly Gln Ile Ala Lys Ile Cys Asn Asn Leu Leu 
                165                 170                 175     


Leu Gly Ile Ser Met Ile Gly Val Ser Glu Ala Met Ala Leu Gly Asn 
            180                 185                 190         


Ala Leu Gly Ile Asp Thr Lys Val Leu Ala Gly Ile Ile Asn Ser Ser 
        195                 200                 205             


Thr Gly Arg Cys Trp Ser Ser Asp Thr Tyr Asn Pro Trp Pro Gly Ile 
    210                 215                 220                 


Ile Glu Thr Ala Pro Ala Ser Arg Gly Tyr Thr Gly Gly Phe Gly Ala 
225                 230                 235                 240 


Glu Leu Met Leu Lys Asp Leu Gly Leu Ala Thr Glu Ala Ala Arg Gln 
                245                 250                 255     


Ala His Gln Pro Val Ile Leu Gly Ala Val Ala Gln Gln Leu Tyr Gln 
            260                 265                 270         


Ala Met Ser Leu Arg Gly Glu Gly Gly Lys Asp Phe Ser Ala Ile Val 
        275                 280                 285             


Glu Gly Tyr Arg Lys Lys Asp Glu Asn Leu Tyr Phe Gln Gly Leu Glu 
    290                 295                 300                 


His His His His His His 
305                 310 


<210>  77
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AS001 (F) Primer

<400>  77
caacatgacc cagtatggcc gtc                                               23


<210>  78
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AS002 (R) Primer

<400>  78
ctacccggga tttcggta                                                     18


<210>  79
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AS003 (F) Primer

<400>  79
caacatgacc gattatggcc gtc                                               23


<210>  80
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AS012 (F) Primer

<400>  80
taccgacgcg gcgcatgaac tggcgcacg                                         29


<210>  81
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AS013 (R) Primer

<400>  81
atcgcttccg cgatgcgt                                                     18


<210>  82
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AS014 (F) Primer

<400>  82
agcgaccatc gcgagcggcc gtac                                              24


<210>  83
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AS015 (R) Primer

<400>  83
tgaacccaaa tgtggttc                                                     18


<210>  84
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AS016 (F) Primer

<400>  84
agcgaccatc aaaagcggcc gta                                               23


<210>  85
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AS017 (R) Primer

<400>  85
tgaacccaaa tgtggttctc                                                   20


<210>  86
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AS018 (F) Primer

<400>  86
ccgtagcggc gcgaccgaaa agc                                               23


<210>  87
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AS019 (R) Primer

<400>  87
atggtcgctt gaacccaa                                                     18


<210>  88
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AS020 (F) Primer

<400>  88
ccgtagcggc aaaaccgaaa agc                                               23


<210>  89
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AS021 (F) Primer

<400>  89
agtgtgggtt gcgattaccg aaatcccggg                                        30


<210>  90
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AS022 (R) Primer

<400>  90
tcctcgttcg ggataccc                                                     18


<210>  91
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AS023 (F) Primer

<400>  91
agtgtgggtt tttattaccg aaatccc                                           27


<210>  92
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AS024 (F) Primer

<400>  92
caacatgacc gcgtatggcc gtctg                                             25


<210>  93
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AS026 (F) Primer

<400>  93
caacatgacc aactatggcc gtctg                                             25


