                         SEQUENCE LISTING

<110>  Board of Regents of the University of Texas System
       Gray, Steven J
       Chen, Xin
 
<120>  OPTIMIZED AP4M1 POLYNUCLEOTIDES AND EXPRESSION CASSETTES AND 
       THEIR USE

<130>  426871-000264

<150>  US 63/177,559
<151>  2021-04-21

<160>  41    

<170>  PatentIn version 3.5

<210>  1
<211>  2309
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hAP4M1 opt vector

<400>  1
ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggggtt cggtacccgc      120

cggcggggcg gagttagggc ggagccaatc agcgtgcgcc gttccgaaag ttgcctttta      180

tggctgggcg gagaatgggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg      240

cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtc gcgcgcgctg      300

tgatcgtcac ttggtaagtc actgactgtc tatgcctggg aaagggtggg caggagatgg      360

ggcagtgcag gaaaagtggc actatgaacc ctgcagccct aggaatgcat ctagacaatt      420

gtactaacct tcttctcttt cctctcctga cagcgtcgac gccaccatga tctcccagtt      480

cttcatcctg tcatcgaagg gcgaccctct gatctacaag gatttccgcg gagactccgg      540

aggacgagat gtggcggaac tcttctaccg gaagctcact ggcctgccgg gagatgagtc      600

cccggtcgtc atgcaccatc acggccgcca tttcatccat attcgccact ctgggctgta      660

cctggtggtc acaaccagcg aaaacgtcag cccgtttagc ctcctggagt tacttagccg      720

cttggccact ctcctgggag actattgtgg ctccctgggc gaaggaacta tctccagaaa      780

cgtggccctg gtgtacgaac tcctcgacga agtgctggac tacggctacg tgcagaccac      840

ctcgaccgag atgctgcgga acttcattca gaccgaggct gtggtgtcga agccttttag      900

cctcttcgac ctctcctctg tgggattgtt tggtgccgaa actcagcagt ccaaggtcgc      960

cccaagctca gccgcctcaa gacctgtgct gagctcgaga tcagatcaga gccagaagaa     1020

cgaggtgttc ctggacgtgg tggaacggct tagcgtcctg atcgcctcca acgggtcgct     1080

gctgaaggtc gacgtccagg gagaaatccg gctgaagtca ttcctcccat cgggctcaga     1140

aatgcggatc ggcctgactg aggagttctg cgtgggaaag agcgaactga gaggatacgg     1200

ccccggcatt agagtggacg aagtctcctt ccattcctcc gtgaacctgg acgagttcga     1260

gtcccaccgc atcctgcggc tccaaccgcc acagggggaa ctgaccgtga tgcgctacca     1320

gctgtccgac gatctgccga gcccccttcc tttccgcttg ttcccgtccg tgcaatggga     1380

taggggctcc ggaaggctcc aagtgtacct taagctgcgc tgcgatctgc tctcgaaaag     1440

ccaggcgctg aacgtgcgcc tgcaccttcc tctgccgagg ggagtggtgt ccctgtccca     1500

agagctgtcc tcgcccgaac agaaggccga gctggcagag ggtgctctcc gctgggacct     1560

acccagagtg cagggaggct cacagctgag cggactgttc cagatggacg tgcccggacc     1620

tcctggaccc ccatcccacg gtctgtccac gtccgcctca cctctgggtc tggggcctgc     1680

aagcttgagc ttcgaacttc cgcggcatac ttgctccggt ctgcaagtcc ggttcctccg     1740

gctggcgttc aggccgtgcg gcaatgccaa cccgcacaaa tgggtccggc acctgtcgca     1800

ctccgacgct tacgtgattc ggatttgata agcgatcgcc agaacctcga ggaccacggc     1860

gcgcctttgc ggatccctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct     1920

cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg     1980

aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc     2040

aggacagcaa gggggaggat tgggaagaca acagcaggca tgctggggat gcggtgggct     2100

ctatggcttc tgaggcggaa agaaccagct ttggacgcgt aggaacccct agtgatggag     2160

ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc     2220

cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctggcgtaat     2280

agcgaagagg cccgcaccga tcgcccttc                                       2309


<210>  2
<211>  106
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' ITR

<400>  2
ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtgg                     106


<210>  3
<211>  164
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  JeT promotor

<400>  3
gggcggagtt agggcggagc caatcagcgt gcgccgttcc gaaagttgcc ttttatggct       60

gggcggagaa tgggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag      120

ctagttccgt cgcagccggg atttgggtcg cggttcttgt ttgt                       164


<210>  4
<211>  156
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Intron

<400>  4
ctgtgatcgt cacttggtaa gtcactgact gtctatgcct gggaaagggt gggcaggaga       60

tggggcagtg caggaaaagt ggcactatga accctgcagc cctaggaatg catctagaca      120

attgtactaa ccttcttctc tttcctctcc tgacag                                156


<210>  5
<211>  1365
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hAP4M1opt

<400>  5
atgatctccc agttcttcat cctgtcatcg aagggcgacc ctctgatcta caaggatttc       60

cgcggagact ccggaggacg agatgtggcg gaactcttct accggaagct cactggcctg      120

ccgggagatg agtccccggt cgtcatgcac catcacggcc gccatttcat ccatattcgc      180

cactctgggc tgtacctggt ggtcacaacc agcgaaaacg tcagcccgtt tagcctcctg      240

gagttactta gccgcttggc cactctcctg ggagactatt gtggctccct gggcgaagga      300

actatctcca gaaacgtggc cctggtgtac gaactcctcg acgaagtgct ggactacggc      360

tacgtgcaga ccacctcgac cgagatgctg cggaacttca ttcagaccga ggctgtggtg      420

tcgaagcctt ttagcctctt cgacctctcc tctgtgggat tgtttggtgc cgaaactcag      480

cagtccaagg tcgccccaag ctcagccgcc tcaagacctg tgctgagctc gagatcagat      540

cagagccaga agaacgaggt gttcctggac gtggtggaac ggcttagcgt cctgatcgcc      600

tccaacgggt cgctgctgaa ggtcgacgtc cagggagaaa tccggctgaa gtcattcctc      660

ccatcgggct cagaaatgcg gatcggcctg actgaggagt tctgcgtggg aaagagcgaa      720

ctgagaggat acggccccgg cattagagtg gacgaagtct ccttccattc ctccgtgaac      780

ctggacgagt tcgagtccca ccgcatcctg cggctccaac cgccacaggg ggaactgacc      840

gtgatgcgct accagctgtc cgacgatctg ccgagccccc ttcctttccg cttgttcccg      900

tccgtgcaat gggatagggg ctccggaagg ctccaagtgt accttaagct gcgctgcgat      960

ctgctctcga aaagccaggc gctgaacgtg cgcctgcacc ttcctctgcc gaggggagtg     1020

gtgtccctgt cccaagagct gtcctcgccc gaacagaagg ccgagctggc agagggtgct     1080

ctccgctggg acctacccag agtgcaggga ggctcacagc tgagcggact gttccagatg     1140

gacgtgcccg gacctcctgg acccccatcc cacggtctgt ccacgtccgc ctcacctctg     1200

ggtctggggc ctgcaagctt gagcttcgaa cttccgcggc atacttgctc cggtctgcaa     1260

gtccggttcc tccggctggc gttcaggccg tgcggcaatg ccaacccgca caaatgggtc     1320

cggcacctgt cgcactccga cgcttacgtg attcggattt gataa                     1365


<210>  6
<211>  254
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BGHpA

<400>  6
ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt       60

gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca      120

ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga      180

ggattgggaa gacaacagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc      240

ggaaagaacc agct                                                        254


<210>  7
<211>  169
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3'-ITR

<400>  7
aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg       60

ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc      120

gagcgcgcag ctggcgtaat agcgaagagg cccgcaccga tcgcccttc                  169


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FGEN-AP4M1 WT

<400>  8
tagaagccag ccatagtggt                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RGEN-AP4M1 WT

<400>  9
gagcaagtgt gtggacctga                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FGEN_AP4M1 mut

<400>  10
cggtcgctac cattaccagt                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RGEN-AP4M1 mut

<400>  11
tgtttcagtg tcccatctgc                                                   20


<210>  12
<211>  106
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of a mutant AAV2 ITR

<400>  12
ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtgg                     106


<210>  13
<211>  169
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of a wildtype AAV2 ITR

<400>  13
aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg       60

ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc      120

gagcgcgcag ctggcgtaat agcgaagagg cccgcaccga tcgcccttc                  169


<210>  14
<211>  130
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of a 5' ITR

<400>  14
ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact      120

aggggttcct                                                             130


<210>  15
<211>  130
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of a 3' ITR

<400>  15
aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg       60

ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc      120

gagcgcgcag                                                             130


<210>  16
<211>  168
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of a 5' ITR

<400>  16
gggggggggg gggggggttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg       60

ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag      120

cgcgcagaga gggagtggcc aactccatca ctaggggttc ctagatct                   168


<210>  17
<211>  168
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of a 5' ITR

<400>  17
agatctagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca       60

ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga      120

gcgagcgagc gcgcagagag ggagtggcca accccccccc cccccccc                   168


<210>  18
<211>  170
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of a 3' ITR

<400>  18
aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg       60

ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc      120

gagcgcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc                 170


<210>  19
<211>  104
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of a 5' ITR

<400>  19
tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc cgggcgtcgg gcgacctttg       60

gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg agtg                       104


<210>  20
<211>  106
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of a 3' ITR

<400>  20
ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac       60

gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcca                     106


<210>  21
<211>  225
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of an MeP229 promoter

<400>  21
caattgaggg cgtcaccgct aaggctccgc cccagcctgg gctccacaac caatgaaggg       60

taatctcgac aaagagcaag gggtggggcg cgggcgcgca ggtgcagcag cacacaggct      120

ggtcgggagg gcggggcgcg acgtctgccg tgcggggtcc cggcatcggt tgcgcgcgcg      180

ctccctcctc tcggagagag ggctgtggta aaacccgtcc ggaaa                      225


<210>  22
<211>  241
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of a U6 promoter

<400>  22
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag       60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga      120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat      180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga      240

c                                                                      241


<210>  23
<211>  448
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of a Synapsin promoter 1

<400>  23
agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga       60

ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga      120

gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg      180

gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc      240

gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg      300

cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg      360

cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg      420

aggagtcgtg tcgtgcctga gagcgcag                                         448


<210>  24
<211>  469
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of a Synapsin promoter 2

<400>  24
ctgcagaggg ccctgcgtat gagtgcaagt gggttttagg accaggatga ggcggggtgg       60

gggtgcctac ctgacgaccg accccgaccc actggacaag cacccaaccc ccattcccca      120

aattgcgcat cccctatcag agagggggag gggaaacagg atgcggcgag gcgcgtgcgc      180

actgccagct tcagcaccgc ggacagtgcc ttcgcccccg cctggcggcg cgcgccaccg      240

ccgcctcagc actgaaggcg cgctgacgtc actcgccggt cccccgcaaa ctccccttcc      300

cggccacctt ggtcgcgtcc gcgccgccgc cggcccagcc ggaccgcacc acgcgaggcg      360

cgagataggg gggcacgggc gcgaccatct gcgctgcggc gccggcgact cagcgctgcc      420

tcagtctgcg gtgggcagcg gaggagtcgt gtcgtgcctg agagcgcag                  469


<210>  25
<211>  302
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of a CBA promoter

<400>  25
acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc       60

ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg      120

cagcgatggg ggcggggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg      180

ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa      240

agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc      300

gg                                                                     302


<210>  26
<211>  332
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of a CBh promoter

<400>  26
ggtaccgggc ggagttaggg cggagccaat cagcgtgcgc cgttccgaaa gttgcctttt       60

atggctgggc ggagaatggg cggtgaacgc cgatgattat ataaggacgc gccgggtgtg      120

gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt ggatccctgt      180

gatcgtcact tggtaagtca ctgactgtct atgcctggga aagggtgggc aggagatggg      240

gcagtgcagg aaaagtggca ctatgaaccc tgcagcccta ggaatgcatc tagacaattg      300

tactaacctt cttctctttc ctctcctgac ag                                    332


<210>  27
<211>  449
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of a hSyn promoter

<400>  27
agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga       60

ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga      120

gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg      180

gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc      240

gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg      300

cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg      360

cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg      420

aggagtcgtg tcgtgcctga gagcgcagt                                        449


<210>  28
<211>  223
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of a MeCP2 promoter

<400>  28
caattgaggg cgtcaccgct aaggctccgc cccagcctgg gctccacaac caatgaaggg       60

taatctcgac aaagagcaag gggtggggcg cgggcgcgca ggtgcagcag cacacaggct      120

ggtcgggagg gcggggcgcg acgtctgccg tgcggggtcc cggcatcggt tgcgcgcgcg      180

ctccctcctc tcggagagag ggctgtggta aaacccgtcc gga                        223


<210>  29
<211>  332
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of a JeT+I promoter

<400>  29
ggtaccgggc ggagttaggg cggagccaat cagcgtgcgc cgttccgaaa gttgcctttt       60

atggctgggc ggagaatggg cggtgaacgc cgatgattat ataaggacgc gccgggtgtg      120

gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt ggatccctgt      180

gatcgtcact tggtaagtca ctgactgtct atgcctggga aagggtgggc aggagatggg      240

gcagtgcagg aaaagtggca ctatgaaccc tgcagcccta ggaatgcatc tagacaattg      300

tactaacctt cttctctttc ctctcctgac ag                                    332


<210>  30
<211>  257
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of a CMV sequence

<400>  30
tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac       60

gtcaatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta      120

aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt      180

caatgacggt aaatggcccg cctggcattg tgcccagtac atgaccttat gggactttcc      240

tacttggcag tacatct                                                     257


<210>  31
<211>  93
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of a CBA Exon 1

<400>  31
ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg       60

ccccggctct gactgaccgc gttactccca cag                                    93


<210>  32
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of a CBA Intron 1

<400>  32
gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agc                         43


<210>  33
<211>  92
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of an MVM Intron

<400>  33
aagaggtaag ggtttaaggg atggttggtt ggtggggtat taatgtttaa ttacctggag       60

cacctgcctg aaatcacttt ttttcaggtt gg                                     92


<210>  34
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of SpA

<400>  34
gcggccgcct cgagaataaa gagctcagat gcatcgatca gagtgtgttg gttttttgtg       60

tg                                                                      62


<210>  35
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of a Kozak sequence

<400>  35
gccaccatgg                                                              10


<210>  36
<211>  586
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of a WPRE

<400>  36
tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc       60

ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat      120

ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg      180

gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg      240

ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat      300

tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt      360

gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc      420

ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa      480

tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg      540

ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccg                     586


<210>  37
<211>  816
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of a kanamycin resistance gene

<400>  37
atgagccata ttcaacggga aacgtcttgc tctaggccgc gattaaattc caacatggat       60

gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc      120

tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc      180

gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct      240

cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg      300

atccctggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt      360

gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct      420

tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg      480

gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa      540

gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca      600

cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc      660

ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct      720

ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa      780

ttgcagtttc atttgatgct cgatgagttt ttctaa                                816


<210>  38
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of a resistance gene promoter

<400>  38
ttcaaatatg tatccgctca tgagacaat                                         29


<210>  39
<211>  328
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of a UsP promoter

<400>  39
gggcggagtt agggcggagc caatcagcgt gcgccgttcc gaaagttgcc ttttatggct       60

gggcggagaa tgggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag      120

ctagttccgt cgcagccggg atttgggtcg cggttcttgt ttgtcgcgcg cgctgtgatc      180

gtcacttggt aagtcactga ctgtctatgc ctgggaaagg gtgggcagga gatggggcag      240

tgcaggaaaa gtggcactat gaaccctgca gccctaggaa tgcatctaga caattgtact      300

aaccttcttc tctttcctct cctgacag                                         328


<210>  40
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hAP4M1opt

<400>  40
atgatctccc agttcttcat cctgtcatcg aagggcgacc ctctgatcta caaggatttc       60

cgcggagact ccggaggacg agatgtggcg gaactcttct accggaagct cactggcctg      120

ccgggagatg agtccccggt cgtcatgcac catcacggcc gccatttcat ccatattcgc      180

cactctgggc tgtacctggt ggtcacaacc agcgaaaacg tcagcccgtt tagcctcctg      240

gagttactta gccgcttggc cactctcctg ggagactatt gtggctccct gggcgaagga      300

actatctcca gaaacgtggc cctggtgtac gaactcctcg acgaagtgct ggactacggc      360

tacgtgcaga ccacctcgac cgagatgctg cggaacttca ttcagaccga ggctgtggtg      420

tcgaagcctt ttagcctctt cgacctctcc tctgtgggat tgtttggtgc cgaaactcag      480

cagtccaagg tcgccccaag ctcagccgcc tcaagacctg tgctgagctc gagatcagat      540

cagagccaga agaacgaggt gttcctggac gtggtggaac ggcttagcgt cctgatcgcc      600

tccaacgggt cgctgctgaa ggtcgacgtc cagggagaaa tccggctgaa gtcattcctc      660

ccatcgggct cagaaatgcg gatcggcctg actgaggagt tctgcgtggg aaagagcgaa      720

ctgagaggat acggccccgg cattagagtg gacgaagtct ccttccattc ctccgtgaac      780

ctggacgagt tcgagtccca ccgcatcctg cggctccaac cgccacaggg ggaactgacc      840

gtgatgcgct accagctgtc cgacgatctg ccgagccccc ttcctttccg cttgttcccg      900

tccgtgcaat gggatagggg ctccggaagg ctccaagtgt accttaagct gcgctgcgat      960

ctgctctcga aaagccaggc gctgaacgtg cgcctgcacc ttcctctgcc gaggggagtg     1020

gtgtccctgt cccaagagct gtcctcgccc gaacagaagg ccgagctggc agagggtgct     1080

ctccgctggg acctacccag agtgcaggga ggctcacagc tgagcggact gttccagatg     1140

gacgtgcccg gacctcctgg acccccatcc cacggtctgt ccacgtccgc ctcacctctg     1200

ggtctggggc ctgcaagctt gagcttcgaa cttccgcggc atacttgctc cggtctgcaa     1260

gtccggttcc tccggctggc gttcaggccg tgcggcaatg ccaacccgca caaatgggtc     1320

cggcacctgt cgcactccga cgcttacgtg attcggattt ga                        1362


<210>  41
<211>  5819
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hAP4M1 opt vector

<400>  41
ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggggtt cggtacccgc      120

cggcggggcg gagttagggc ggagccaatc agcgtgcgcc gttccgaaag ttgcctttta      180

tggctgggcg gagaatgggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg      240

cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtc gcgcgcgctg      300

tgatcgtcac ttggtaagtc actgactgtc tatgcctggg aaagggtggg caggagatgg      360

ggcagtgcag gaaaagtggc actatgaacc ctgcagccct aggaatgcat ctagacaatt      420

gtactaacct tcttctcttt cctctcctga cagcgtcgac gccaccatga tctcccagtt      480

cttcatcctg tcatcgaagg gcgaccctct gatctacaag gatttccgcg gagactccgg      540

aggacgagat gtggcggaac tcttctaccg gaagctcact ggcctgccgg gagatgagtc      600

cccggtcgtc atgcaccatc acggccgcca tttcatccat attcgccact ctgggctgta      660

cctggtggtc acaaccagcg aaaacgtcag cccgtttagc ctcctggagt tacttagccg      720

cttggccact ctcctgggag actattgtgg ctccctgggc gaaggaacta tctccagaaa      780

cgtggccctg gtgtacgaac tcctcgacga agtgctggac tacggctacg tgcagaccac      840

ctcgaccgag atgctgcgga acttcattca gaccgaggct gtggtgtcga agccttttag      900

cctcttcgac ctctcctctg tgggattgtt tggtgccgaa actcagcagt ccaaggtcgc      960

cccaagctca gccgcctcaa gacctgtgct gagctcgaga tcagatcaga gccagaagaa     1020

cgaggtgttc ctggacgtgg tggaacggct tagcgtcctg atcgcctcca acgggtcgct     1080

gctgaaggtc gacgtccagg gagaaatccg gctgaagtca ttcctcccat cgggctcaga     1140

aatgcggatc ggcctgactg aggagttctg cgtgggaaag agcgaactga gaggatacgg     1200

ccccggcatt agagtggacg aagtctcctt ccattcctcc gtgaacctgg acgagttcga     1260

gtcccaccgc atcctgcggc tccaaccgcc acagggggaa ctgaccgtga tgcgctacca     1320

gctgtccgac gatctgccga gcccccttcc tttccgcttg ttcccgtccg tgcaatggga     1380

taggggctcc ggaaggctcc aagtgtacct taagctgcgc tgcgatctgc tctcgaaaag     1440

ccaggcgctg aacgtgcgcc tgcaccttcc tctgccgagg ggagtggtgt ccctgtccca     1500

agagctgtcc tcgcccgaac agaaggccga gctggcagag ggtgctctcc gctgggacct     1560

acccagagtg cagggaggct cacagctgag cggactgttc cagatggacg tgcccggacc     1620

tcctggaccc ccatcccacg gtctgtccac gtccgcctca cctctgggtc tggggcctgc     1680

aagcttgagc ttcgaacttc cgcggcatac ttgctccggt ctgcaagtcc ggttcctccg     1740

gctggcgttc aggccgtgcg gcaatgccaa cccgcacaaa tgggtccggc acctgtcgca     1800

ctccgacgct tacgtgattc ggatttgata agcgatcgcc agaacctcga ggaccacggc     1860

gcgcctttgc ggatccctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct     1920

cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg     1980

aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc     2040

aggacagcaa gggggaggat tgggaagaca acagcaggca tgctggggat gcggtgggct     2100

ctatggcttc tgaggcggaa agaaccagct ttggacgcgt aggaacccct agtgatggag     2160

ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc     2220

cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctggcgtaat     2280

agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg     2340

cgattccgtt gcaatggctg gcggtaatat tgttctggat attaccagca aggccgatag     2400

tttgagttct tctactcagg caagtgatgt tattactaat caaagaagta ttgcgacaac     2460

ggttaatttg cgtgatggac agactctttt actcggtggc ctcactgatt ataaaaacac     2520

ttctcaggat tctggcgtac cgttcctgtc taaaatccct ttaatcggcc tcctgtttag     2580

ctcccgctct gattctaacg aggaaagcac gttatacgtg ctcgtcaaag caaccatagt     2640

acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg     2700

ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca     2760

cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta     2820

gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc     2880

catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg     2940

gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat     3000

aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta     3060

acgcgaattt taacaaaata ttaacgctta caatttaaat atttgcttat acaatcttcc     3120

tgtttttggg gcttttctga ttatcaaccg gggtacatat gattgacatg ctagttttac     3180

gattaccgtt catcgattct cttgtttgct ccagactctc aggcaatgac ctgatagcct     3240

ttgtagagac ctctcaaaaa tagctaccct ctccggcatg aatttatcag ctagaacggt     3300

tgaatatcat attgatggtg atttgactgt ctccggcctt tctcacccgt ttgaatcttt     3360

acctacacat tactcaggca ttgcatttaa aatatatgag ggttctaaaa atttttatcc     3420

ttgcgttgaa ataaaggctt ctcccgcaaa agtattacag ggtcataatg tttttggtac     3480

aaccgattta gctttatgct ctgaggcttt attgcttaat tttgctaatt ctttgccttg     3540

cctgtatgat ttattggatg ttggaatcgc ctgatgcggt attttctcct tacgcatctg     3600

tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag     3660

ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc     3720

ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt     3780

tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag     3840

gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg     3900

cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga     3960

caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag ccatattcaa     4020

cgggaaacgt cttgctctag gccgcgatta aattccaaca tggatgctga tttatatggg     4080

tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg attgtatggg     4140

aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt     4200

acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag     4260

cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc tggcaaaaca     4320

gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca     4380

gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc     4440

gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat     4500

tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt     4560

ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt     4620

tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga     4680

taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa     4740

cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg     4800

atgctcgatg agtttttcta ataactgtca gaccaagttt actcatatat actttagatt     4860

gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc     4920

atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag     4980

atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa     5040

aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg     5100

aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag     5160

ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg     5220

ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga     5280

tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc     5340

ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc     5400

acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga     5460

gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt     5520

cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg     5580

aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac     5640

atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga     5700

gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg     5760

gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcag      5819


