                         SEQUENCE LISTING

<110>  The Trustees of the University of Pennsylvania
       Mason, Nicola J.
       Siegel, Donald L.
       Chester, Nicholas
 
<120>  Canine Monoclonal Antibodies Against Canine Cytotoxic T 
       Lymphocyte Associated Protein 4 (CTLA-4)

<130>  046483-7312WO1(02862)

<150>  U.S. Provisional Patent Application No. 63/177,692
<151>  2021-04-21

<160>  77    

<170>  PatentIn version 3.5

<210>  1
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1 HCDR1

<400>  1

Gly Phe Ser Phe Ser Ser Tyr Ala 
1               5               


<210>  2
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1 HCDR2

<400>  2

Ile Asn Ser Gly Gly Ser Ser Thr 
1               5               


<210>  3
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1 HCDR3

<400>  3

Ala Ile Ser Asn Trp Ala Tyr 
1               5           


<210>  4
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1 LCDR1

<400>  4

Ser Ser Asp Ile Gly Lys Ser Tyr 
1               5               


<210>  5
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1 LCDR3

<400>  5

Ser Ser Trp Asp Trp Ser Leu His Thr Tyr Val 
1               5                   10      


<210>  6
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1mut2 VH

<400>  6

Glu Val Gln Leu Val Glu Thr Gly Gly Asp Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Phe Ser Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Asn Trp Val Arg Gln Ala Pro Glu Lys Gly Leu Gln Leu Val 
        35                  40                  45              


Gly Gly Ile Asn Ser Gly Gly Ser Ser Thr Tyr Tyr Thr Asp Ala Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ile Ser Asn Trp Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val 
            100                 105                 110         


Ser Ser 
        


<210>  7
<211>  342
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A1mut2 VH

<400>  7
gaggtgcagc tggtggagac cgggggagac ctggtgaagc ctggcgggtc cctgagattg       60

tcctgtgtgg cctctggatt ctccttcagc agttatgcca tgaactgggt ccgccaggct      120

cctgagaagg ggctgcagct ggtgggcggt attaatagcg gtggaagtag tacatattac      180

accgacgctg tgaagggccg cttcaccatc tccagagaca acgccaagaa cacagtgtat      240

ttacagatga atagcctgag agccgaggac acggccgtgt attactgtgc gattagtaat      300

tgggcctact ggggccaggg aaccctggtc accgtctcct ca                         342


<210>  8
<211>  110
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1 VL

<400>  8

Gln Ala Val Leu Asn Gln Pro Ala Ser Val Ser Gly Ser Leu Gly Gln 
1               5                   10                  15      


Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asp Ile Gly Lys Ser 
            20                  25                  30          


Tyr Val Ala Trp Tyr Gln Gln Leu Pro Gly Thr Gly Pro Arg Thr Leu 
        35                  40                  45              


Ile Asn Val Asp Gly Asn Arg Ala Ser Gly Val Pro Asp Arg Phe Ser 
    50                  55                  60                  


Val Ser Arg Ser Gly Asn Thr Ala Thr Leu Thr Ile Ser Gly Leu Gln 
65                  70                  75                  80  


Ala Glu Asp Glu Ala Asp Tyr His Cys Ser Ser Trp Asp Trp Ser Leu 
                85                  90                  95      


His Thr Tyr Val Phe Gly Ser Gly Thr Gln Leu Thr Ile Leu 
            100                 105                 110 


<210>  9
<211>  330
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A1 VL

<400>  9
caggctgtgc tgaatcagcc ggcctcagtg tccgggtccc tgggccagag ggtcaccatc       60

tcctgcactg gaagcagctc cgacatcggt aaaagttatg tggcctggta ccagcagctc      120

ccgggaacag gccccagaac cctcatcaat gttgatggta accgagcctc aggggtcccc      180

gatcgattct ctgtctccag gtcaggcaac acagccaccc tgaccatctc cgggctccag      240

gctgaggatg aggctgatta tcactgctca tcctgggact ggagtctcca tacttacgtg      300

ttcggctcag ggacccagct gaccatcctc                                       330


<210>  10
<211>  465
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1mut2 heavy chain full-length cIgG1

<400>  10

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser Glu Val Gln Leu Val Glu Thr Gly Gly Asp Leu Val 
            20                  25                  30          


Lys Pro Gly Gly Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Phe Ser 
        35                  40                  45              


Phe Ser Ser Tyr Ala Met Asn Trp Val Arg Gln Ala Pro Glu Lys Gly 
    50                  55                  60                  


Leu Gln Leu Val Gly Gly Ile Asn Ser Gly Gly Ser Ser Thr Tyr Tyr 
65                  70                  75                  80  


Thr Asp Ala Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                85                  90                  95      


Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Ile Ser Asn Trp Ala Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ser Ala Ser Thr Thr Ala Pro Ser Val Phe Pro 
    130                 135                 140                 


Leu Ala Pro Ser Cys Gly Ser Thr Ser Gly Ser Thr Val Ala Leu Ala 
145                 150                 155                 160 


Cys Leu Val Ser Gly Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
                165                 170                 175     


Ser Gly Ser Leu Thr Ser Gly Val His Thr Phe Pro Ser Val Leu Gln 
            180                 185                 190         


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Met Val Thr Val Pro Ser Ser 
        195                 200                 205             


Arg Trp Pro Ser Glu Thr Phe Thr Cys Asn Val Val His Pro Ala Ser 
    210                 215                 220                 


Asn Thr Lys Val Asp Lys Pro Val Phe Asn Glu Cys Arg Cys Thr Asp 
225                 230                 235                 240 


Thr Pro Pro Cys Pro Val Pro Glu Pro Leu Gly Gly Pro Ser Val Leu 
                245                 250                 255     


Ile Phe Pro Pro Lys Pro Lys Asp Ile Leu Arg Ile Thr Arg Thr Pro 
            260                 265                 270         


Glu Val Thr Cys Val Val Leu Asp Leu Gly Arg Glu Asp Pro Glu Val 
        275                 280                 285             


Gln Ile Ser Trp Phe Val Asp Gly Lys Glu Val His Thr Ala Lys Thr 
    290                 295                 300                 


Gln Ser Arg Glu Gln Gln Phe Asn Gly Thr Tyr Arg Val Val Ser Val 
305                 310                 315                 320 


Leu Pro Ile Glu His Gln Asp Trp Leu Thr Gly Lys Glu Phe Lys Cys 
                325                 330                 335     


Arg Val Asn His Ile Asp Leu Pro Ser Pro Ile Glu Arg Thr Ile Ser 
            340                 345                 350         


Lys Ala Arg Gly Arg Ala His Lys Pro Ser Val Tyr Val Leu Pro Pro 
        355                 360                 365             


Ser Pro Lys Glu Leu Ser Ser Ser Asp Thr Val Ser Val Thr Cys Leu 
    370                 375                 380                 


Ile Lys Asp Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Ser Asn 
385                 390                 395                 400 


Gly Gln Gln Glu Pro Glu Arg Lys His Arg Met Thr Pro Pro Gln Leu 
                405                 410                 415     


Asp Glu Asp Gly Ser Tyr Phe Leu Tyr Ser Lys Leu Ser Val Asp Lys 
            420                 425                 430         


Ser Arg Trp Gln Gln Gly Asp Pro Phe Thr Cys Ala Val Met His Glu 
        435                 440                 445             


Thr Leu Gln Asn His Tyr Thr Asp Leu Ser Leu Ser His Ser Pro Gly 
    450                 455                 460                 


Lys 
465 


<210>  11
<211>  1395
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A1mut2 heavy chain full-length cIgG1

<400>  11
atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg       60

gaggtgcagc tggtggagac cgggggagac ctggtgaagc ctggcgggtc cctgagattg      120

tcctgtgtgg cctctggatt ctccttcagc agttatgcca tgaactgggt ccgccaggct      180

cctgagaagg ggctgcagct ggtgggcggt attaatagcg gtggaagtag tacatattac      240

accgacgctg tgaagggccg cttcaccatc tccagagaca acgccaagaa cacagtgtat      300

ttacagatga atagcctgag agccgaggac acggccgtgt attactgtgc gattagtaat      360

tgggcctact ggggccaggg aaccctggtc accgtctcct cagctagcac cacggccccc      420

tcggttttcc cactggcccc cagctgcggg tccacttccg gctccacggt ggccctggcc      480

tgcctggtgt caggctactt ccccgagcct gtaactgtgt cctggaactc cggctccttg      540

accagcggtg tgcacacctt cccgtccgtc ctgcagtcct cagggctcta ctccctcagc      600

agcatggtga cagtgccctc cagcagatgg cccagtgaga ccttcacctg caacgtggtc      660

cacccggcca gcaacactaa agtagacaag ccagtgttca atgaatgcag atgcactgat      720

acacccccat gcccagtccc tgaacctctg ggagggcctt cggtcctcat ctttcccccg      780

aaacccaagg acatcctcag gattacccga acacccgagg tcacctgtgt ggtgttagat      840

ctgggccgtg aggaccctga ggtgcagatc agctggttcg tggatggtaa ggaggtgcac      900

acagccaaga cgcagtctcg tgagcagcag ttcaacggca cctaccgtgt ggtcagcgtc      960

ctccccattg agcaccagga ctggctcaca gggaaggagt tcaagtgcag agtcaaccac     1020

atagacctcc catctcccat cgagaggacc atctctaagg ccagagggag ggcccataag     1080

cccagtgtgt atgtcctgcc accatcccca aaggagttgt catccagtga cacagtcagc     1140

gtcacctgcc tgataaaaga cttctaccca cctgacattg atgtggagtg gcagagcaat     1200

ggacagcagg agcctgagag gaagcaccgc atgaccccgc cccagctgga cgaggacggg     1260

tcctacttcc tgtacagcaa gctctctgtg gacaagagcc gctggcagca gggagacccc     1320

ttcacatgtg cggtgatgca tgaaactcta cagaaccact acacagatct atccctctcc     1380

cattctccgg gtaaa                                                      1395


<210>  12
<211>  469
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1mut2 heavy chain full-length cIgG2

<400>  12

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser Glu Val Gln Leu Val Glu Thr Gly Gly Asp Leu Val 
            20                  25                  30          


Lys Pro Gly Gly Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Phe Ser 
        35                  40                  45              


Phe Ser Ser Tyr Ala Met Asn Trp Val Arg Gln Ala Pro Glu Lys Gly 
    50                  55                  60                  


Leu Gln Leu Val Gly Gly Ile Asn Ser Gly Gly Ser Ser Thr Tyr Tyr 
65                  70                  75                  80  


Thr Asp Ala Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                85                  90                  95      


Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Ile Ser Asn Trp Ala Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ser Ala Ser Thr Thr Ala Pro Ser Val Phe Pro 
    130                 135                 140                 


Leu Ala Pro Ser Cys Gly Ser Thr Ser Gly Ser Thr Val Ala Leu Ala 
145                 150                 155                 160 


Cys Leu Val Ser Gly Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
                165                 170                 175     


Ser Gly Ser Leu Thr Ser Gly Val His Thr Phe Pro Ser Val Leu Gln 
            180                 185                 190         


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Met Val Thr Val Pro Ser Ser 
        195                 200                 205             


Arg Trp Pro Ser Glu Thr Phe Thr Cys Asn Val Ala His Pro Ala Ser 
    210                 215                 220                 


Lys Thr Lys Val Asp Lys Pro Val Pro Lys Arg Glu Asn Gly Arg Val 
225                 230                 235                 240 


Pro Arg Pro Pro Asp Cys Pro Lys Cys Pro Ala Pro Glu Met Leu Gly 
                245                 250                 255     


Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Leu 
            260                 265                 270         


Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Leu Asp Pro 
        275                 280                 285             


Glu Asp Pro Glu Val Gln Ile Ser Trp Phe Val Asp Gly Lys Gln Met 
    290                 295                 300                 


Gln Thr Ala Lys Thr Gln Pro Arg Glu Glu Gln Phe Asn Gly Thr Tyr 
305                 310                 315                 320 


Arg Val Val Ser Val Leu Pro Ile Gly His Gln Asp Trp Leu Lys Gly 
                325                 330                 335     


Lys Gln Phe Thr Cys Lys Val Asn Asn Lys Ala Leu Pro Ser Pro Ile 
            340                 345                 350         


Glu Arg Thr Ile Ser Lys Ala Arg Gly Gln Ala His Gln Pro Ser Val 
        355                 360                 365             


Tyr Val Leu Pro Pro Ser Arg Glu Glu Leu Ser Lys Asn Thr Val Ser 
    370                 375                 380                 


Leu Thr Cys Leu Ile Lys Asp Phe Phe Pro Pro Asp Ile Asp Val Glu 
385                 390                 395                 400 


Trp Gln Ser Asn Gly Gln Gln Glu Pro Glu Ser Lys Tyr Arg Thr Thr 
                405                 410                 415     


Pro Pro Gln Leu Asp Glu Asp Gly Ser Tyr Phe Leu Tyr Ser Lys Leu 
            420                 425                 430         


Ser Val Asp Lys Ser Arg Trp Gln Arg Gly Asp Thr Phe Ile Cys Ala 
        435                 440                 445             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Glu Ser Leu Ser 
    450                 455                 460                 


His Ser Pro Gly Lys 
465                 


<210>  13
<211>  1407
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A1mut2 heavy chain full-length cIgG2

<400>  13
atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg       60

gaggtgcagc tggtggagac cgggggagac ctggtgaagc ctggcgggtc cctgagattg      120

tcctgtgtgg cctctggatt ctccttcagc agttatgcca tgaactgggt ccgccaggct      180

cctgagaagg ggctgcagct ggtgggcggt attaatagcg gtggaagtag tacatattac      240

accgacgctg tgaagggccg cttcaccatc tccagagaca acgccaagaa cacagtgtat      300

ttacagatga atagcctgag agccgaggac acggccgtgt attactgtgc gattagtaat      360

tgggcctact ggggccaggg aaccctggtc accgtctcct cagctagcac cacggccccc      420

tcggttttcc cactggcccc cagctgcggg tccacttccg gctccacggt ggccctggcc      480

tgcctggtgt caggctactt ccccgagcct gtaactgtgt cctggaactc cggctccttg      540

accagcggtg tgcacacctt cccgtccgtc ctgcagtcct cagggctcta ctccctcagc      600

agcatggtga cagtgccctc cagcaggtgg cccagcgaga ccttcacctg caacgtggcc      660

cacccggcca gcaaaactaa agtagacaag ccagtgccca aaagagaaaa tggaagagtt      720

cctcgcccac ctgattgtcc caaatgccca gcccctgaaa tgctgggagg gccttcggtc      780

ttcatctttc ccccgaaacc caaggacacc ctcttgattg cccgaacacc tgaggtcaca      840

tgtgtggtgg tggatctgga cccagaagac cctgaggtgc agatcagctg gttcgtggac      900

ggtaagcaga tgcaaacagc caagactcag cctcgtgagg agcagttcaa tggcacctac      960

cgtgtggtca gtgtcctccc cattgggcac caggactggc tcaaggggaa gcagttcacg     1020

tgcaaagtca acaacaaagc cctcccatcc ccgatcgaga ggaccatctc caaggccaga     1080

gggcaggccc atcagcccag tgtgtatgtc ctgccgccat cccgggagga gttgagcaag     1140

aacacagtca gcttgacatg cctgatcaaa gacttcttcc cacctgacat tgatgtggag     1200

tggcagagca atggacagca ggagcctgag agcaagtacc gcacgacccc gccccagctg     1260

gacgaggacg ggtcctactt cctgtacagc aagctctctg tggacaagag ccgctggcag     1320

cggggagaca ccttcatatg tgcggtgatg catgaagctc tacacaacca ctacacacag     1380

gaatccctct cccattctcc gggtaaa                                         1407


<210>  14
<211>  467
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1mut2 heavy chain full-length cIgG3

<400>  14

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser Glu Val Gln Leu Val Glu Thr Gly Gly Asp Leu Val 
            20                  25                  30          


Lys Pro Gly Gly Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Phe Ser 
        35                  40                  45              


Phe Ser Ser Tyr Ala Met Asn Trp Val Arg Gln Ala Pro Glu Lys Gly 
    50                  55                  60                  


Leu Gln Leu Val Gly Gly Ile Asn Ser Gly Gly Ser Ser Thr Tyr Tyr 
65                  70                  75                  80  


Thr Asp Ala Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                85                  90                  95      


Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Ile Ser Asn Trp Ala Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ser Ala Ser Thr Thr Ala Pro Ser Val Phe Pro 
    130                 135                 140                 


Leu Ala Pro Ser Cys Gly Ser Gln Ser Gly Ser Thr Val Ala Leu Ala 
145                 150                 155                 160 


Cys Leu Val Ser Gly Tyr Ile Pro Glu Pro Val Thr Val Ser Trp Asn 
                165                 170                 175     


Ser Gly Ser Leu Thr Ser Gly Val His Thr Phe Pro Ser Ile Leu Gln 
            180                 185                 190         


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Met Val Thr Val Pro Ser Ser 
        195                 200                 205             


Arg Trp Pro Ser Glu Thr Phe Thr Cys Asn Val Ala His Pro Ala Thr 
    210                 215                 220                 


Asn Thr Lys Val Asp Lys Pro Val Val Lys Glu Cys Glu Cys Lys Cys 
225                 230                 235                 240 


Asn Cys Asn Asn Cys Pro Cys Pro Gly Cys Gly Leu Leu Gly Gly Pro 
                245                 250                 255     


Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Ile Leu Val Thr Ala 
            260                 265                 270         


Arg Thr Pro Thr Val Thr Cys Val Val Val Asp Leu Asp Pro Glu Asn 
        275                 280                 285             


Pro Glu Val Gln Ile Ser Trp Phe Val Asp Ser Lys Gln Val Gln Thr 
    290                 295                 300                 


Ala Asn Thr Gln Pro Arg Glu Glu Gln Ser Asn Gly Thr Tyr Arg Val 
305                 310                 315                 320 


Val Ser Val Leu Pro Ile Gly His Gln Asp Trp Leu Ser Gly Lys Gln 
                325                 330                 335     


Phe Lys Cys Lys Val Asn Asn Lys Ala Leu Pro Ser Pro Ile Glu Glu 
            340                 345                 350         


Ile Ile Ser Lys Thr Pro Gly Gln Ala His Gln Pro Asn Val Tyr Val 
        355                 360                 365             


Leu Pro Pro Ser Arg Asp Glu Met Ser Lys Asn Thr Val Thr Leu Thr 
    370                 375                 380                 


Cys Leu Val Lys Asp Phe Phe Pro Pro Glu Ile Asp Val Glu Trp Gln 
385                 390                 395                 400 


Ser Asn Gly Gln Gln Glu Pro Glu Ser Lys Tyr Arg Met Thr Pro Pro 
                405                 410                 415     


Gln Leu Asp Glu Asp Gly Ser Tyr Phe Leu Tyr Ser Lys Leu Ser Val 
            420                 425                 430         


Asp Lys Ser Arg Trp Gln Arg Gly Asp Thr Phe Ile Cys Ala Val Met 
        435                 440                 445             


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser His Ser 
    450                 455                 460                 


Pro Gly Lys 
465         


<210>  15
<211>  1401
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A1mut2 heavy chain full-length cIgG3

<400>  15
atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg       60

gaggtgcagc tggtggagac cgggggagac ctggtgaagc ctggcgggtc cctgagattg      120

tcctgtgtgg cctctggatt ctccttcagc agttatgcca tgaactgggt ccgccaggct      180

cctgagaagg ggctgcagct ggtgggcggt attaatagcg gtggaagtag tacatattac      240

accgacgctg tgaagggccg cttcaccatc tccagagaca acgccaagaa cacagtgtat      300

ttacagatga atagcctgag agccgaggac acggccgtgt attactgtgc gattagtaat      360

tgggcctact ggggccaggg aaccctggtc accgtctcct cagctagcac cacggccccc      420

tcggttttcc cactggcccc cagctgtggg tcccaatccg gctccacggt ggccctggcc      480

tgcctggtgt caggctacat ccccgagcct gtaactgtgt cctggaactc cggctccttg      540

accagcggtg tgcacacctt cccgtccatc ctgcagtcct cagggctcta ctccctcagc      600

agcatggtga cagtgccctc cagcaggtgg cccagcgaga ccttcacctg caatgtggcc      660

cacccggcca ccaacactaa agtagacaag ccagtggtca aagaatgcga gtgcaagtgt      720

aactgtaaca actgcccatg cccaggttgt ggcctgctgg gagggccttc ggtcttcatc      780

tttcccccaa aacccaagga catcctcgtg actgcccgga cacccacagt cacttgtgtg      840

gtggtggatc tggacccaga aaaccctgag gtgcagatca gctggttcgt ggatagtaag      900

caggtgcaaa cagccaacac gcagcctcgt gaggagcagt ccaatggcac ctaccgtgtg      960

gtcagtgtcc tccccattgg gcaccaggac tggctttcag ggaagcagtt caagtgcaaa     1020

gtcaacaaca aagccctccc atcccccatt gaggagatca tctccaagac cccagggcag     1080

gcccatcagc ctaatgtgta tgtcctgccg ccatcgcggg atgagatgag caagaatacg     1140

gtcaccctga cctgtctggt caaagacttc ttcccacctg agattgatgt ggagtggcag     1200

agcaatggac agcaggagcc tgagagcaag taccgcatga ccccgcccca gctggatgag     1260

gatgggtcct acttcctata cagcaagctc tctgtggaca agagccgctg gcagcgggga     1320

gacaccttca tatgtgcggt gatgcatgaa gctctacaca accactacac acagaaatcc     1380

ctctcccatt ctccgggtaa a                                               1401


<210>  16
<211>  465
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1mut2 heavy chain full-length cIgG4

<400>  16

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser Glu Val Gln Leu Val Glu Thr Gly Gly Asp Leu Val 
            20                  25                  30          


Lys Pro Gly Gly Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Phe Ser 
        35                  40                  45              


Phe Ser Ser Tyr Ala Met Asn Trp Val Arg Gln Ala Pro Glu Lys Gly 
    50                  55                  60                  


Leu Gln Leu Val Gly Gly Ile Asn Ser Gly Gly Ser Ser Thr Tyr Tyr 
65                  70                  75                  80  


Thr Asp Ala Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                85                  90                  95      


Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Ile Ser Asn Trp Ala Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ser Ala Ser Thr Thr Ala Pro Ser Val Phe Pro 
    130                 135                 140                 


Leu Ala Pro Ser Cys Gly Ser Thr Ser Gly Ser Thr Val Ala Leu Ala 
145                 150                 155                 160 


Cys Leu Val Ser Gly Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
                165                 170                 175     


Ser Gly Ser Leu Thr Ser Gly Val His Thr Phe Pro Ser Val Leu Gln 
            180                 185                 190         


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Met Val Thr Val Pro Ser Ser 
        195                 200                 205             


Arg Trp Pro Ser Glu Thr Phe Thr Cys Asn Val Val His Pro Ala Ser 
    210                 215                 220                 


Asn Thr Lys Val Asp Lys Pro Val Pro Lys Glu Ser Thr Cys Lys Cys 
225                 230                 235                 240 


Ile Ser Pro Cys Pro Val Pro Glu Ser Leu Gly Gly Pro Ser Val Phe 
                245                 250                 255     


Ile Phe Pro Pro Lys Pro Lys Asp Ile Leu Arg Ile Thr Arg Thr Pro 
            260                 265                 270         


Glu Ile Thr Cys Val Val Leu Asp Leu Gly Arg Glu Asp Pro Glu Val 
        275                 280                 285             


Gln Ile Ser Trp Phe Val Asp Gly Lys Glu Val His Thr Ala Lys Thr 
    290                 295                 300                 


Gln Pro Arg Glu Gln Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val 
305                 310                 315                 320 


Leu Pro Ile Glu His Gln Asp Trp Leu Thr Gly Lys Glu Phe Lys Cys 
                325                 330                 335     


Arg Val Asn His Ile Gly Leu Pro Ser Pro Ile Glu Arg Thr Ile Ser 
            340                 345                 350         


Lys Ala Arg Gly Gln Ala His Gln Pro Ser Val Tyr Val Leu Pro Pro 
        355                 360                 365             


Ser Pro Lys Glu Leu Ser Ser Ser Asp Thr Val Thr Leu Thr Cys Leu 
    370                 375                 380                 


Ile Lys Asp Phe Phe Pro Pro Glu Ile Asp Val Glu Trp Gln Ser Asn 
385                 390                 395                 400 


Gly Gln Pro Glu Pro Glu Ser Lys Tyr His Thr Thr Ala Pro Gln Leu 
                405                 410                 415     


Asp Glu Asp Gly Ser Tyr Phe Leu Tyr Ser Lys Leu Ser Val Asp Lys 
            420                 425                 430         


Ser Arg Trp Gln Gln Gly Asp Pro Phe Thr Cys Ala Val Met His Glu 
        435                 440                 445             


Ala Leu Gln Asn His Tyr Thr Asp Leu Ser Leu Ser His Ser Pro Gly 
    450                 455                 460                 


Lys 
465 


<210>  17
<211>  1395
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A1mut2 heavy chain full-length cIgG4

<400>  17
atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg       60

gaggtgcagc tggtggagac cgggggagac ctggtgaagc ctggcgggtc cctgagattg      120

tcctgtgtgg cctctggatt ctccttcagc agttatgcca tgaactgggt ccgccaggct      180

cctgagaagg ggctgcagct ggtgggcggt attaatagcg gtggaagtag tacatattac      240

accgacgctg tgaagggccg cttcaccatc tccagagaca acgccaagaa cacagtgtat      300

ttacagatga atagcctgag agccgaggac acggccgtgt attactgtgc gattagtaat      360

tgggcctact ggggccaggg aaccctggtc accgtctcct cagctagcac cacggccccc      420

tcggttttcc cactggcccc cagctgcggg tccacttccg gctccacggt ggccctagcc      480

tgcctggtgt caggctactt ccccgagcct gtaactgtgt cctggaactc cggctccttg      540

accagcggtg tgcacacctt cccgtccgtc ctgcagtcct cagggctcta ctccctcagc      600

agcatggtga cagtgccctc cagcaggtgg cccagcgaga ccttcacctg caacgtggtc      660

cacccggcca gcaacactaa agtagacaag ccagtgccca aagagtccac ctgcaagtgt      720

atatccccat gcccagtccc tgaatcactg ggagggcctt cggtcttcat ctttcccccg      780

aaacccaagg acatcctcag gattacccga acacccgaga tcacctgtgt ggtgttagat      840

ctgggccgtg aggaccctga ggtgcagatc agctggttcg tggatggtaa ggaggtgcac      900

acagccaaga cgcagcctcg tgagcagcag ttcaacagca cctaccgtgt ggtcagcgtc      960

ctccccattg agcaccagga ctggctcacc ggaaaggagt tcaagtgcag agtcaaccac     1020

ataggcctcc cgtcccccat cgagaggacc atctccaaag ccagagggca agcccatcag     1080

cccagtgtgt atgtcctgcc accatcccca aaggagttgt catccagtga cacggtcacc     1140

ctgacctgcc tgatcaaaga cttcttccca cctgagattg atgtggagtg gcagagcaat     1200

ggacagccag agcctgagag caagtaccac acgactgcac cccagctgga cgaggacggg     1260

tcctacttcc tgtacagcaa gctctctgtg gacaagagcc gctggcagca gggagacccc     1320

ttcacatgtg cggtgatgca tgaagctcta cagaaccact acacagatct atccctctcc     1380

cattctccgg gtaaa                                                      1395


<210>  18
<211>  236
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1mut2 lambda light chain full-length

<400>  18

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser Gln Ala Val Leu Asn Gln Pro Ala Ser Val Ser Gly 
            20                  25                  30          


Ser Leu Gly Gln Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asp 
        35                  40                  45              


Ile Gly Lys Ser Tyr Val Ala Trp Tyr Gln Gln Leu Pro Gly Thr Gly 
    50                  55                  60                  


Pro Arg Thr Leu Ile Asn Val Asp Gly Asn Arg Ala Ser Gly Val Pro 
65                  70                  75                  80  


Asp Arg Phe Ser Val Ser Arg Ser Gly Asn Thr Ala Thr Leu Thr Ile 
                85                  90                  95      


Ser Gly Leu Gln Ala Glu Asp Glu Ala Asp Tyr His Cys Ser Ser Trp 
            100                 105                 110         


Asp Trp Ser Leu His Thr Tyr Val Phe Gly Ser Gly Thr Gln Leu Thr 
        115                 120                 125             


Ile Leu Gly Gln Pro Lys Ala Ser Pro Ser Val Thr Leu Phe Pro Pro 
    130                 135                 140                 


Ser Ser Glu Glu Leu Gly Ala Asn Lys Ala Thr Leu Val Cys Leu Ile 
145                 150                 155                 160 


Ser Asp Phe Tyr Pro Ser Gly Val Thr Val Ala Trp Lys Ala Asp Gly 
                165                 170                 175     


Ser Pro Ile Thr Gln Gly Val Glu Thr Thr Lys Pro Ser Lys Gln Ser 
            180                 185                 190         


Asn Asn Lys Tyr Ala Ala Ser Ser Tyr Leu Ser Leu Thr Pro Asp Lys 
        195                 200                 205             


Trp Lys Ser His Ser Ser Phe Ser Cys Leu Val Thr His Glu Gly Ser 
    210                 215                 220                 


Thr Val Glu Lys Lys Val Ala Pro Ala Glu Cys Ser 
225                 230                 235     


<210>  19
<211>  709
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A1mut2 lambda light chain full-length

<400>  19
atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg       60

caggctgtgc tgaatcagcc ggcctcagtg tccgggtccc tgggccagag ggtcaccatc      120

tcctgcactg gaagcagctc cgacatcggt aaaagttatg tggcctggta ccagcagctc      180

ccgggaacag gccccagaac cctcatcaat gttgatggta accgagcctc aggggtcccc      240

gatcgattct ctgtctccag gtcaggcaac acagccaccc tgaccatctc cgggctccag      300

gctgaggatg aggctgatta tcactgctca tcctgggact ggagtctcca tacttacgtg      360

ttcggctcag ggacccagct gaccatccta ggtcagccca aggcctcccc ctcggtcaca      420

ctcttcccgc cctcctctga ggagctcggc gccaacaagg ccaccctggt gtgcctcatc      480

agcgacttct accccagcgg cgtgacggtg gcctggaagg cagacggcag ccccatcacc      540

cagggcgtgg agaccaccaa gccctccaag cagagcaaca acaagtacgc ggccagcagc      600

tacctgagcc tgacgcctga caagtggaaa tctcacagca gcttcagctg cctggtcacg      660

cacgagggga gcaccgtgga gaagaaggtg gcccccgcag agtgctctt                  709


<210>  20
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  D5 HCDR1

<400>  20

Gly Phe Thr Phe Ser Ser Tyr Ser 
1               5               


<210>  21
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  D5 HCDR2

<400>  21

Ile Asn Ser Gly Gly Ser Ser Thr 
1               5               


<210>  22
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  D5 HCDR3

<400>  22

Val Ile Ser Asn Trp Ser Tyr 
1               5           


<210>  23
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  D5 LCDR1

<400>  23

Ala Thr Asn Val Gly Gly Gly Tyr Asp 
1               5                   


<210>  24
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  D5 LCDR3

<400>  24

Ser Ser Trp Asp Asn Ser Leu Thr Ala Tyr Val 
1               5                   10      


<210>  25
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  D5 VH

<400>  25

Glu Val Gln Leu Val Glu Thr Gly Gly Asp Leu Met Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ser Met Ser Trp Val Arg Gln Ala Pro Glu Lys Gly Leu Gln Leu Val 
        35                  40                  45              


Ala Gly Ile Asn Ser Gly Gly Ser Ser Thr Tyr Tyr Thr Asp Ala Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Asp Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Val Ile Ser Asn Trp Ser Tyr Trp Gly Gln Gly Thr Leu Val Thr Val 
            100                 105                 110         


Ser Ser 
        


<210>  26
<211>  342
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  D5 VH

<400>  26
gaggtgcagc tggtggagac tgggggagac ctgatgaagc ctggggggtc cctgagactg       60

tcctgtgtgg cctctggatt caccttcagt agctacagta tgagttgggt ccgccaggct      120

cctgagaagg ggctgcagtt ggtcgcaggt attaacagcg gtggaagtag cacatactac      180

acagacgctg tgaagggccg attcaccatc tccagagaca acgccaagaa cacgctgtat      240

ctgcagatga acagtctgag agatgaagac acggcagtct attattgtgt gatcagtaat      300

tggtcctact ggggccaggg aaccctggtc accgtctcct ca                         342


<210>  27
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  D5 VL

<400>  27

Gln Pro Val Leu Thr Gln Pro Pro Ser Val Ser Ala Ala Leu Gly Gln 
1               5                   10                  15      


Arg Val Thr Ile Ser Cys Thr Gly Thr Ala Thr Asn Val Gly Gly Gly 
            20                  25                  30          


Tyr Asp Val Gln Trp Tyr Gln Gln Phe Pro Gly Arg Pro Pro Lys Thr 
        35                  40                  45              


Ile Ile Tyr Gly Asn Tyr Asn Arg Pro Ser Gly Val Pro Asp Arg Phe 
    50                  55                  60                  


Ser Ala Ser Thr Ser Gly Thr Thr Ala Thr Leu Thr Ile Ser Gly Leu 
65                  70                  75                  80  


Gln Ala Glu Asp Glu Ala Asn Tyr Tyr Cys Ser Ser Trp Asp Asn Ser 
                85                  90                  95      


Leu Thr Ala Tyr Val Phe Gly Ser Gly Thr Gln Leu Thr Ile Leu 
            100                 105                 110     


<210>  28
<211>  333
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  D5 VL

<400>  28
cagcctgtgc tcactcagcc gccctctgtg tctgcggccc tgggacagag ggtcaccatc       60

tcctgcactg gaactgcgac caacgtcggc ggcggttatg atgtacaatg gtaccagcag      120

tttccaggaa gaccccctaa aactatcatt tacggtaatt acaatcgccc ctcgggggtc      180

ccagatcgat tctctgcctc cacgtcaggc accacagcca ccctgaccat ctctgggctc      240

caggctgagg atgaggctaa ttattactgc tcatcgtggg acaacagtct cactgcttac      300

gttttcggct cagggaccca gctgaccatc ctc                                   333


<210>  29
<211>  277
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  D5 scFv

<400>  29

Gln Pro Val Leu Thr Gln Pro Pro Ser Val Ser Ala Ala Leu Gly Gln 
1               5                   10                  15      


Arg Val Thr Ile Ser Cys Thr Gly Thr Ala Thr Asn Val Gly Gly Gly 
            20                  25                  30          


Tyr Asp Val Gln Trp Tyr Gln Gln Phe Pro Gly Arg Pro Pro Lys Thr 
        35                  40                  45              


Ile Ile Tyr Gly Asn Tyr Asn Arg Pro Ser Gly Val Pro Asp Arg Phe 
    50                  55                  60                  


Ser Ala Ser Thr Ser Gly Thr Thr Ala Thr Leu Thr Ile Ser Gly Leu 
65                  70                  75                  80  


Gln Ala Glu Asp Glu Ala Asn Tyr Tyr Cys Ser Ser Trp Asp Asn Ser 
                85                  90                  95      


Leu Thr Ala Tyr Val Phe Gly Ser Gly Thr Gln Leu Thr Ile Leu Gly 
            100                 105                 110         


Gly Gly Ser Ser Arg Ser Ser Ser Ser Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             


Gly Gly Glu Val Gln Leu Val Glu Thr Gly Gly Asp Leu Met Lys Pro 
    130                 135                 140                 


Gly Gly Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Phe Thr Phe Ser 
145                 150                 155                 160 


Ser Tyr Ser Met Ser Trp Val Arg Gln Ala Pro Glu Lys Gly Leu Gln 
                165                 170                 175     


Leu Val Ala Gly Ile Asn Ser Gly Gly Ser Ser Thr Tyr Tyr Thr Asp 
            180                 185                 190         


Ala Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr 
        195                 200                 205             


Leu Tyr Leu Gln Met Asn Ser Leu Arg Asp Glu Asp Thr Ala Val Tyr 
    210                 215                 220                 


Tyr Cys Val Ile Ser Asn Trp Ser Tyr Trp Gly Gln Gly Thr Leu Val 
225                 230                 235                 240 


Thr Val Ser Ser Glu Ser Pro Ser Pro Pro Asn Leu Thr Ser Gly Gln 
                245                 250                 255     


Ala Gly Gln His His His His His His Gly Ala Tyr Pro Tyr Asp Val 
            260                 265                 270         


Pro Asp Tyr Ala Ser 
        275         


<210>  30
<211>  831
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  D5 scFv

<400>  30
cagcctgtgc tcactcagcc gccctctgtg tctgcggccc tgggacagag ggtcaccatc       60

tcctgcactg gaactgcgac caacgtcggc ggcggttatg atgtacaatg gtaccagcag      120

tttccaggaa gaccccctaa aactatcatt tacggtaatt acaatcgccc ctcgggggtc      180

ccagatcgat tctctgcctc cacgtcaggc accacagcca ccctgaccat ctctgggctc      240

caggctgagg atgaggctaa ttattactgc tcatcgtggg acaacagtct cactgcttac      300

gttttcggct cagggaccca gctgaccatc ctcggcggtg gttcctctag atcttcctcc      360

tctggtggcg gtggctcggg cggtggtggg gaggtgcagc tggtggagac tgggggagac      420

ctgatgaagc ctggggggtc cctgagactg tcctgtgtgg cctctggatt caccttcagt      480

agctacagta tgagttgggt ccgccaggct cctgagaagg ggctgcagtt ggtcgcaggt      540

attaacagcg gtggaagtag cacatactac acagacgctg tgaagggccg attcaccatc      600

tccagagaca acgccaagaa cacgctgtat ctgcagatga acagtctgag agatgaagac      660

acggcagtct attattgtgt gatcagtaat tggtcctact ggggccaggg aaccctggtc      720

accgtctcct cagagagtcc atcccctcca aacctcacta gtggccaggc cggccagcac      780

catcaccatc accatggcgc atacccgtac gacgttccgg actacgcttc t               831


<210>  31
<211>  465
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  D5 heavy chain full-length cIgG1

<400>  31

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser Glu Val Gln Leu Val Glu Thr Gly Gly Asp Leu Met 
            20                  25                  30          


Lys Pro Gly Gly Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Phe Thr 
        35                  40                  45              


Phe Ser Ser Tyr Ser Met Ser Trp Val Arg Gln Ala Pro Glu Lys Gly 
    50                  55                  60                  


Leu Gln Leu Val Ala Gly Ile Asn Ser Gly Gly Ser Ser Thr Tyr Tyr 
65                  70                  75                  80  


Thr Asp Ala Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                85                  90                  95      


Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Asp Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Val Ile Ser Asn Trp Ser Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ser Ala Ser Thr Thr Ala Pro Ser Val Phe Pro 
    130                 135                 140                 


Leu Ala Pro Ser Cys Gly Ser Thr Ser Gly Ser Thr Val Ala Leu Ala 
145                 150                 155                 160 


Cys Leu Val Ser Gly Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
                165                 170                 175     


Ser Gly Ser Leu Thr Ser Gly Val His Thr Phe Pro Ser Val Leu Gln 
            180                 185                 190         


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Met Val Thr Val Pro Ser Ser 
        195                 200                 205             


Arg Trp Pro Ser Glu Thr Phe Thr Cys Asn Val Val His Pro Ala Ser 
    210                 215                 220                 


Asn Thr Lys Val Asp Lys Pro Val Phe Asn Glu Cys Arg Cys Thr Asp 
225                 230                 235                 240 


Thr Pro Pro Cys Pro Val Pro Glu Pro Leu Gly Gly Pro Ser Val Leu 
                245                 250                 255     


Ile Phe Pro Pro Lys Pro Lys Asp Ile Leu Arg Ile Thr Arg Thr Pro 
            260                 265                 270         


Glu Val Thr Cys Val Val Leu Asp Leu Gly Arg Glu Asp Pro Glu Val 
        275                 280                 285             


Gln Ile Ser Trp Phe Val Asp Gly Lys Glu Val His Thr Ala Lys Thr 
    290                 295                 300                 


Gln Ser Arg Glu Gln Gln Phe Asn Gly Thr Tyr Arg Val Val Ser Val 
305                 310                 315                 320 


Leu Pro Ile Glu His Gln Asp Trp Leu Thr Gly Lys Glu Phe Lys Cys 
                325                 330                 335     


Arg Val Asn His Ile Asp Leu Pro Ser Pro Ile Glu Arg Thr Ile Ser 
            340                 345                 350         


Lys Ala Arg Gly Arg Ala His Lys Pro Ser Val Tyr Val Leu Pro Pro 
        355                 360                 365             


Ser Pro Lys Glu Leu Ser Ser Ser Asp Thr Val Ser Val Thr Cys Leu 
    370                 375                 380                 


Ile Lys Asp Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Ser Asn 
385                 390                 395                 400 


Gly Gln Gln Glu Pro Glu Arg Lys His Arg Met Thr Pro Pro Gln Leu 
                405                 410                 415     


Asp Glu Asp Gly Ser Tyr Phe Leu Tyr Ser Lys Leu Ser Val Asp Lys 
            420                 425                 430         


Ser Arg Trp Gln Gln Gly Asp Pro Phe Thr Cys Ala Val Met His Glu 
        435                 440                 445             


Thr Leu Gln Asn His Tyr Thr Asp Leu Ser Leu Ser His Ser Pro Gly 
    450                 455                 460                 


Lys 
465 


<210>  32
<211>  1395
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  D5 heavy chain full-length cIgG1

<400>  32
atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg       60

gaggtgcagc tggtggagac tgggggagac ctgatgaagc ctggggggtc cctgagactg      120

tcctgtgtgg cctctggatt caccttcagt agctacagta tgagttgggt ccgccaggct      180

cctgagaagg ggctgcagtt ggtcgcaggt attaacagcg gtggaagtag cacatactac      240

acagacgctg tgaagggccg attcaccatc tccagagaca acgccaagaa cacgctgtat      300

ctgcagatga acagtctgag agatgaagac acggcagtct attattgtgt gatcagtaat      360

tggtcctact ggggccaggg aaccctggtc accgtctcct cagctagcac cacggccccc      420

tcggttttcc cactggcccc cagctgcggg tccacttccg gctccacggt ggccctggcc      480

tgcctggtgt caggctactt ccccgagcct gtaactgtgt cctggaactc cggctccttg      540

accagcggtg tgcacacctt cccgtccgtc ctgcagtcct cagggctcta ctccctcagc      600

agcatggtga cagtgccctc cagcagatgg cccagtgaga ccttcacctg caacgtggtc      660

cacccggcca gcaacactaa agtagacaag ccagtgttca atgaatgcag atgcactgat      720

acacccccat gcccagtccc tgaacctctg ggagggcctt cggtcctcat ctttcccccg      780

aaacccaagg acatcctcag gattacccga acacccgagg tcacctgtgt ggtgttagat      840

ctgggccgtg aggaccctga ggtgcagatc agctggttcg tggatggtaa ggaggtgcac      900

acagccaaga cgcagtctcg tgagcagcag ttcaacggca cctaccgtgt ggtcagcgtc      960

ctccccattg agcaccagga ctggctcaca gggaaggagt tcaagtgcag agtcaaccac     1020

atagacctcc catctcccat cgagaggacc atctctaagg ccagagggag ggcccataag     1080

cccagtgtgt atgtcctgcc accatcccca aaggagttgt catccagtga cacagtcagc     1140

gtcacctgcc tgataaaaga cttctaccca cctgacattg atgtggagtg gcagagcaat     1200

ggacagcagg agcctgagag gaagcaccgc atgaccccgc cccagctgga cgaggacggg     1260

tcctacttcc tgtacagcaa gctctctgtg gacaagagcc gctggcagca gggagacccc     1320

ttcacatgtg cggtgatgca tgaaactcta cagaaccact acacagatct atccctctcc     1380

cattctccgg gtaaa                                                      1395


<210>  33
<211>  237
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  D5 lambda light chain full-length

<400>  33

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser Gln Pro Val Leu Thr Gln Pro Pro Ser Val Ser Ala 
            20                  25                  30          


Ala Leu Gly Gln Arg Val Thr Ile Ser Cys Thr Gly Thr Ala Thr Asn 
        35                  40                  45              


Val Gly Gly Gly Tyr Asp Val Gln Trp Tyr Gln Gln Phe Pro Gly Arg 
    50                  55                  60                  


Pro Pro Lys Thr Ile Ile Tyr Gly Asn Tyr Asn Arg Pro Ser Gly Val 
65                  70                  75                  80  


Pro Asp Arg Phe Ser Ala Ser Thr Ser Gly Thr Thr Ala Thr Leu Thr 
                85                  90                  95      


Ile Ser Gly Leu Gln Ala Glu Asp Glu Ala Asn Tyr Tyr Cys Ser Ser 
            100                 105                 110         


Trp Asp Asn Ser Leu Thr Ala Tyr Val Phe Gly Ser Gly Thr Gln Leu 
        115                 120                 125             


Thr Ile Leu Gly Gln Pro Lys Ala Ser Pro Ser Val Thr Leu Phe Pro 
    130                 135                 140                 


Pro Ser Ser Glu Glu Leu Gly Ala Asn Lys Ala Thr Leu Val Cys Leu 
145                 150                 155                 160 


Ile Ser Asp Phe Tyr Pro Ser Gly Val Thr Val Ala Trp Lys Ala Asp 
                165                 170                 175     


Gly Ser Pro Ile Thr Gln Gly Val Glu Thr Thr Lys Pro Ser Lys Gln 
            180                 185                 190         


Ser Asn Asn Lys Tyr Ala Ala Ser Ser Tyr Leu Ser Leu Thr Pro Asp 
        195                 200                 205             


Lys Trp Lys Ser His Ser Ser Phe Ser Cys Leu Val Thr His Glu Gly 
    210                 215                 220                 


Ser Thr Val Glu Lys Lys Val Ala Pro Ala Glu Cys Ser 
225                 230                 235         


<210>  34
<211>  712
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  D5 lambda light chain full-length

<400>  34
atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg       60

cagcctgtgc tcactcagcc gccctctgtg tctgcggccc tgggacagag ggtcaccatc      120

tcctgcactg gaactgcgac caacgtcggc ggcggttatg atgtacaatg gtaccagcag      180

tttccaggaa gaccccctaa aactatcatt tacggtaatt acaatcgccc ctcgggggtc      240

ccagatcgat tctctgcctc cacgtcaggc accacagcca ccctgaccat ctctgggctc      300

caggctgagg atgaggctaa ttattactgc tcatcgtggg acaacagtct cactgcttac      360

gttttcggct cagggaccca gctgaccatc ctcggtcagc ccaaggcctc cccctcggtc      420

acactcttcc cgccctcctc tgaggagctc ggcgccaaca aggccaccct ggtgtgcctc      480

atcagcgact tctaccccag cggcgtgacg gtggcctgga aggcagacgg cagccccatc      540

acccagggcg tggagaccac caagccctcc aagcagagca acaacaagta cgcggccagc      600

agctacctga gcctgacgcc tgacaagtgg aaatctcaca gcagcttcag ctgcctggtc      660

acgcacgagg ggagcaccgt ggagaagaag gtggcccccg cagagtgctc tt              712


<210>  35
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B10 HCDR1

<400>  35

Gly Phe Thr Phe Ser Asp Tyr Pro 
1               5               


<210>  36
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B10 HCDR2

<400>  36

Ile Asn Ser Gly Gly Ser Ala Thr 
1               5               


<210>  37
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B10 HCDR3

<400>  37

Ala Thr Ser Asn Phe Gln Tyr 
1               5           


<210>  38
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B10 LCDR1

<400>  38

Asn Thr Asn Ile Gly Ser Pro Tyr Asp 
1               5                   


<210>  39
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B10 LCDR3

<400>  39

Gln Ser Tyr Asp Asp Asn Val Asp Gly Tyr Val 
1               5                   10      


<210>  40
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B10 VH

<400>  40

Glu Val Gln Leu Val Glu Thr Gly Gly Asp Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Phe Thr Phe Ser Asp Tyr 
            20                  25                  30          


Pro Met Asn Trp Val Arg Gln Ala Pro Glu Lys Gly Leu Gln Leu Val 
        35                  40                  45              


Gly Gly Ile Asn Ser Gly Gly Ser Ala Thr Tyr Tyr Thr Asp Ala Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Thr Ser Asn Phe Gln Tyr Trp Gly Gln Gly Thr Leu Val Thr Val 
            100                 105                 110         


Ser Ser 
        


<210>  41
<211>  342
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B10 VH

<400>  41
gaggtgcagc tggtggagac tgggggagat ctggtgaagc ctgggggatc cctgagactc       60

tcttgtgtgg cctctggatt caccttcagt gactacccca tgaactgggt ccgccaggct      120

cctgagaagg ggctgcagtt ggtcggtggt attaacagcg gtggaagtgc tacatactac      180

acagacgctg tgaagggccg attcaccatc tccagagaca acgccaagaa cacagtgtat      240

ctgcagatga acagcctgag agccgaggac acggccatgt attactgtgc aacgtctaat      300

tttcagtact ggggccaggg aaccctggtc accgtctcct ca                         342


<210>  42
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B10 VL

<400>  42

Gln Ala Val Leu Asn Gln Pro Ala Ser Val Ser Ala Ala Leu Gly Gln 
1               5                   10                  15      


Arg Val Thr Ile Ser Cys Asn Thr Asn Ile Gly Ser Pro Tyr Asp Val 
            20                  25                  30          


Gln Trp Tyr Gln Gln Leu Pro Gly Lys Ser Pro Lys Thr Ile Ile Tyr 
        35                  40                  45              


Gly Asn Ser Asn Arg Pro Ser Gly Val Pro Val Arg Phe Ser Gly Ser 
    50                  55                  60                  


Lys Ser Gly Ser Thr Ala Thr Leu Thr Ile Ala Gly Ile Gln Ala Glu 
65                  70                  75                  80  


Asp Glu Ala Asp Tyr Tyr Cys Gln Ser Tyr Asp Asp Asn Val Asp Gly 
                85                  90                  95      


Tyr Val Phe Gly Ser Gly Thr Gln Leu Thr Val Leu 
            100                 105             


<210>  43
<211>  324
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B10 VL

<400>  43
caggctgtgc tgaatcagcc ggcctctgtg tctgcagccc tggggcagag ggtcaccatc       60

tcctgtaaca ccaacatcgg cagtccttat gatgtacaat ggtaccagca gctcccagga      120

aagtccccta aaactatcat ttatggtaat agcaatcgac cctcgggggt cccggttcga      180

ttctctggct ccaagtcagg cagcacagcc accctgacca tcgctgggat ccaggctgag      240

gatgaggctg attattactg ccagtcctat gatgacaacg tcgatggtta cgtgttcggc      300

tcagggaccc aactgaccgt cctt                                             324


<210>  44
<211>  274
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B10 scFv

<400>  44

Gln Ala Val Leu Asn Gln Pro Ala Ser Val Ser Ala Ala Leu Gly Gln 
1               5                   10                  15      


Arg Val Thr Ile Ser Cys Asn Thr Asn Ile Gly Ser Pro Tyr Asp Val 
            20                  25                  30          


Gln Trp Tyr Gln Gln Leu Pro Gly Lys Ser Pro Lys Thr Ile Ile Tyr 
        35                  40                  45              


Gly Asn Ser Asn Arg Pro Ser Gly Val Pro Val Arg Phe Ser Gly Ser 
    50                  55                  60                  


Lys Ser Gly Ser Thr Ala Thr Leu Thr Ile Ala Gly Ile Gln Ala Glu 
65                  70                  75                  80  


Asp Glu Ala Asp Tyr Tyr Cys Gln Ser Tyr Asp Asp Asn Val Asp Gly 
                85                  90                  95      


Tyr Val Phe Gly Ser Gly Thr Gln Leu Thr Val Leu Gly Gly Gly Ser 
            100                 105                 110         


Ser Arg Ser Ser Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Glu 
        115                 120                 125             


Val Gln Leu Val Glu Thr Gly Gly Asp Leu Val Lys Pro Gly Gly Ser 
    130                 135                 140                 


Leu Arg Leu Ser Cys Val Ala Ser Gly Phe Thr Phe Ser Asp Tyr Pro 
145                 150                 155                 160 


Met Asn Trp Val Arg Gln Ala Pro Glu Lys Gly Leu Gln Leu Val Gly 
                165                 170                 175     


Gly Ile Asn Ser Gly Gly Ser Ala Thr Tyr Tyr Thr Asp Ala Val Lys 
            180                 185                 190         


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr Leu 
        195                 200                 205             


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Met Tyr Tyr Cys Ala 
    210                 215                 220                 


Thr Ser Asn Phe Gln Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser 
225                 230                 235                 240 


Ser Glu Ser Pro Ser Pro Pro Asn Leu Thr Ser Gly Gln Ala Gly Gln 
                245                 250                 255     


His His His His His His Gly Ala Tyr Pro Tyr Asp Val Pro Asp Tyr 
            260                 265                 270         


Ala Ser 
        


<210>  45
<211>  822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B10 scFv

<400>  45
caggctgtgc tgaatcagcc ggcctctgtg tctgcagccc tggggcagag ggtcaccatc       60

tcctgtaaca ccaacatcgg cagtccttat gatgtacaat ggtaccagca gctcccagga      120

aagtccccta aaactatcat ttatggtaat agcaatcgac cctcgggggt cccggttcga      180

ttctctggct ccaagtcagg cagcacagcc accctgacca tcgctgggat ccaggctgag      240

gatgaggctg attattactg ccagtcctat gatgacaacg tcgatggtta cgtgttcggc      300

tcagggaccc aactgaccgt ccttggcggt ggttcctcta gatcttcctc ctctggtggc      360

ggtggctcgg gcggtggtgg ggaggtgcag ctggtggaga ctgggggaga tctggtgaag      420

cctgggggat ccctgagact ctcttgtgtg gcctctggat tcaccttcag tgactacccc      480

atgaactggg tccgccaggc tcctgagaag gggctgcagt tggtcggtgg tattaacagc      540

ggtggaagtg ctacatacta cacagacgct gtgaagggcc gattcaccat ctccagagac      600

aacgccaaga acacagtgta tctgcagatg aacagcctga gagccgagga cacggccatg      660

tattactgtg caacgtctaa ttttcagtac tggggccagg gaaccctggt caccgtctcc      720

tcagagagtc catcccctcc aaacctcact agtggccagg ccggccagca ccatcaccat      780

caccatggcg catacccgta cgacgttccg gactacgctt ct                         822


<210>  46
<211>  465
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B10 heavy chain full-length cIgG1

<400>  46

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser Glu Val Gln Leu Val Glu Thr Gly Gly Asp Leu Val 
            20                  25                  30          


Lys Pro Gly Gly Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Phe Thr 
        35                  40                  45              


Phe Ser Asp Tyr Pro Met Asn Trp Val Arg Gln Ala Pro Glu Lys Gly 
    50                  55                  60                  


Leu Gln Leu Val Gly Gly Ile Asn Ser Gly Gly Ser Ala Thr Tyr Tyr 
65                  70                  75                  80  


Thr Asp Ala Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                85                  90                  95      


Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            100                 105                 110         


Met Tyr Tyr Cys Ala Thr Ser Asn Phe Gln Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ser Ala Ser Thr Thr Ala Pro Ser Val Phe Pro 
    130                 135                 140                 


Leu Ala Pro Ser Cys Gly Ser Thr Ser Gly Ser Thr Val Ala Leu Ala 
145                 150                 155                 160 


Cys Leu Val Ser Gly Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
                165                 170                 175     


Ser Gly Ser Leu Thr Ser Gly Val His Thr Phe Pro Ser Val Leu Gln 
            180                 185                 190         


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Met Val Thr Val Pro Ser Ser 
        195                 200                 205             


Arg Trp Pro Ser Glu Thr Phe Thr Cys Asn Val Val His Pro Ala Ser 
    210                 215                 220                 


Asn Thr Lys Val Asp Lys Pro Val Phe Asn Glu Cys Arg Cys Thr Asp 
225                 230                 235                 240 


Thr Pro Pro Cys Pro Val Pro Glu Pro Leu Gly Gly Pro Ser Val Leu 
                245                 250                 255     


Ile Phe Pro Pro Lys Pro Lys Asp Ile Leu Arg Ile Thr Arg Thr Pro 
            260                 265                 270         


Glu Val Thr Cys Val Val Leu Asp Leu Gly Arg Glu Asp Pro Glu Val 
        275                 280                 285             


Gln Ile Ser Trp Phe Val Asp Gly Lys Glu Val His Thr Ala Lys Thr 
    290                 295                 300                 


Gln Ser Arg Glu Gln Gln Phe Asn Gly Thr Tyr Arg Val Val Ser Val 
305                 310                 315                 320 


Leu Pro Ile Glu His Gln Asp Trp Leu Thr Gly Lys Glu Phe Lys Cys 
                325                 330                 335     


Arg Val Asn His Ile Asp Leu Pro Ser Pro Ile Glu Arg Thr Ile Ser 
            340                 345                 350         


Lys Ala Arg Gly Arg Ala His Lys Pro Ser Val Tyr Val Leu Pro Pro 
        355                 360                 365             


Ser Pro Lys Glu Leu Ser Ser Ser Asp Thr Val Ser Val Thr Cys Leu 
    370                 375                 380                 


Ile Lys Asp Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Ser Asn 
385                 390                 395                 400 


Gly Gln Gln Glu Pro Glu Arg Lys His Arg Met Thr Pro Pro Gln Leu 
                405                 410                 415     


Asp Glu Asp Gly Ser Tyr Phe Leu Tyr Ser Lys Leu Ser Val Asp Lys 
            420                 425                 430         


Ser Arg Trp Gln Gln Gly Asp Pro Phe Thr Cys Ala Val Met His Glu 
        435                 440                 445             


Thr Leu Gln Asn His Tyr Thr Asp Leu Ser Leu Ser His Ser Pro Gly 
    450                 455                 460                 


Lys 
465 


<210>  47
<211>  1395
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B10 heavy chain full-length cIgG1

<400>  47
atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg       60

gaggtgcagc tggtggagac tgggggagat ctggtgaagc ctgggggatc cctgagactc      120

tcttgtgtgg cctctggatt caccttcagt gactacccca tgaactgggt ccgccaggct      180

cctgagaagg ggctgcagtt ggtcggtggt attaacagcg gtggaagtgc tacatactac      240

acagacgctg tgaagggccg attcaccatc tccagagaca acgccaagaa cacagtgtat      300

ctgcagatga acagcctgag agccgaggac acggccatgt attactgtgc aacgtctaat      360

tttcagtact ggggccaggg aaccctggtc accgtctcct cagctagcac cacggccccc      420

tcggttttcc cactggcccc cagctgcggg tccacttccg gctccacggt ggccctggcc      480

tgcctggtgt caggctactt ccccgagcct gtaactgtgt cctggaactc cggctccttg      540

accagcggtg tgcacacctt cccgtccgtc ctgcagtcct cagggctcta ctccctcagc      600

agcatggtga cagtgccctc cagcagatgg cccagtgaga ccttcacctg caacgtggtc      660

cacccggcca gcaacactaa agtagacaag ccagtgttca atgaatgcag atgcactgat      720

acacccccat gcccagtccc tgaacctctg ggagggcctt cggtcctcat ctttcccccg      780

aaacccaagg acatcctcag gattacccga acacccgagg tcacctgtgt ggtgttagat      840

ctgggccgtg aggaccctga ggtgcagatc agctggttcg tggatggtaa ggaggtgcac      900

acagccaaga cgcagtctcg tgagcagcag ttcaacggca cctaccgtgt ggtcagcgtc      960

ctccccattg agcaccagga ctggctcaca gggaaggagt tcaagtgcag agtcaaccac     1020

atagacctcc catctcccat cgagaggacc atctctaagg ccagagggag ggcccataag     1080

cccagtgtgt atgtcctgcc accatcccca aaggagttgt catccagtga cacagtcagc     1140

gtcacctgcc tgataaaaga cttctaccca cctgacattg atgtggagtg gcagagcaat     1200

ggacagcagg agcctgagag gaagcaccgc atgaccccgc cccagctgga cgaggacggg     1260

tcctacttcc tgtacagcaa gctctctgtg gacaagagcc gctggcagca gggagacccc     1320

ttcacatgtg cggtgatgca tgaaactcta cagaaccact acacagatct atccctctcc     1380

cattctccgg gtaaa                                                      1395


<210>  48
<211>  234
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B10 lambda light chain full-length

<400>  48

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser Gln Ala Val Leu Asn Gln Pro Ala Ser Val Ser Ala 
            20                  25                  30          


Ala Leu Gly Gln Arg Val Thr Ile Ser Cys Asn Thr Asn Ile Gly Ser 
        35                  40                  45              


Pro Tyr Asp Val Gln Trp Tyr Gln Gln Leu Pro Gly Lys Ser Pro Lys 
    50                  55                  60                  


Thr Ile Ile Tyr Gly Asn Ser Asn Arg Pro Ser Gly Val Pro Val Arg 
65                  70                  75                  80  


Phe Ser Gly Ser Lys Ser Gly Ser Thr Ala Thr Leu Thr Ile Ala Gly 
                85                  90                  95      


Ile Gln Ala Glu Asp Glu Ala Asp Tyr Tyr Cys Gln Ser Tyr Asp Asp 
            100                 105                 110         


Asn Val Asp Gly Tyr Val Phe Gly Ser Gly Thr Gln Leu Thr Val Leu 
        115                 120                 125             


Gly Gln Pro Lys Ala Ser Pro Ser Val Thr Leu Phe Pro Pro Ser Ser 
    130                 135                 140                 


Glu Glu Leu Gly Ala Asn Lys Ala Thr Leu Val Cys Leu Ile Ser Asp 
145                 150                 155                 160 


Phe Tyr Pro Ser Gly Val Thr Val Ala Trp Lys Ala Asp Gly Ser Pro 
                165                 170                 175     


Ile Thr Gln Gly Val Glu Thr Thr Lys Pro Ser Lys Gln Ser Asn Asn 
            180                 185                 190         


Lys Tyr Ala Ala Ser Ser Tyr Leu Ser Leu Thr Pro Asp Lys Trp Lys 
        195                 200                 205             


Ser His Ser Ser Phe Ser Cys Leu Val Thr His Glu Gly Ser Thr Val 
    210                 215                 220                 


Glu Lys Lys Val Ala Pro Ala Glu Cys Ser 
225                 230                 


<210>  49
<211>  703
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B10 lambda light chain full-length

<400>  49
atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg       60

caggctgtgc tgaatcagcc ggcctctgtg tctgcagccc tggggcagag ggtcaccatc      120

tcctgtaaca ccaacatcgg cagtccttat gatgtacaat ggtaccagca gctcccagga      180

aagtccccta aaactatcat ttatggtaat agcaatcgac cctcgggggt cccggttcga      240

ttctctggct ccaagtcagg cagcacagcc accctgacca tcgctgggat ccaggctgag      300

gatgaggctg attattactg ccagtcctat gatgacaacg tcgatggtta cgtgttcggc      360

tcagggaccc aactgaccgt ccttggtcag cccaaggcct ccccctcggt cacactcttc      420

ccgccctcct ctgaggagct cggcgccaac aaggccaccc tggtgtgcct catcagcgac      480

ttctacccca gcggcgtgac ggtggcctgg aaggcagacg gcagccccat cacccagggc      540

gtggagacca ccaagccctc caagcagagc aacaacaagt acgcggccag cagctacctg      600

agcctgacgc ctgacaagtg gaaatctcac agcagcttca gctgcctggt cacgcacgag      660

gggagcaccg tggagaagaa ggtggccccc gcagagtgct ctt                        703


<210>  50
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Full Length Canine CTLA-4

<400>  50

Met Ala Gly Phe Gly Phe Arg Arg His Gly Ala Gln Pro Asp Leu Ala 
1               5                   10                  15      


Ser Arg Thr Trp Pro Cys Thr Ala Leu Phe Ser Leu Leu Phe Ile Pro 
            20                  25                  30          


Val Phe Ser Lys Gly Met His Val Ala Gln Pro Ala Val Val Leu Ala 
        35                  40                  45              


Ser Ser Arg Gly Val Ala Ser Phe Val Cys Glu Tyr Gly Ser Ser Gly 
    50                  55                  60                  


Asn Ala Ala Glu Val Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln 
65                  70                  75                  80  


Met Thr Glu Val Cys Ala Ala Thr Tyr Thr Val Glu Asp Glu Leu Ala 
                85                  90                  95      


Phe Leu Asp Asp Ser Thr Cys Thr Gly Thr Ser Ser Gly Asn Lys Val 
            100                 105                 110         


Asn Leu Thr Ile Gln Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile 
        115                 120                 125             


Cys Lys Val Glu Leu Met Tyr Pro Pro Pro Tyr Tyr Val Gly Met Gly 
    130                 135                 140                 


Asn Gly Thr Gln Ile Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser 
145                 150                 155                 160 


Asp Phe Leu Leu Trp Ile Leu Ala Ala Val Ser Ser Gly Leu Phe Phe 
                165                 170                 175     


Tyr Ser Phe Leu Ile Thr Ala Val Ser Leu Ser Lys Met Leu Lys Lys 
            180                 185                 190         


Arg Ser Pro Leu Thr Thr Gly Val Tyr Val Lys Met Pro Pro Thr Glu 
        195                 200                 205             


Pro Glu Cys Glu Lys Gln Phe Gln Pro Tyr Phe Ile Pro Ile Asn 
    210                 215                 220             


<210>  51
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL2 signal sequence

<400>  51

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser 
            20  


<210>  52
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Canine lambda-1 light chain

<400>  52

Gly Gln Pro Lys Ala Ser Pro Ser Val Thr Leu Phe Pro Pro Ser Ser 
1               5                   10                  15      


Glu Glu Leu Gly Ala Asn Lys Ala Thr Leu Val Cys Leu Ile Ser Asp 
            20                  25                  30          


Phe Tyr Pro Ser Gly Val Thr Val Ala Trp Lys Ala Asp Gly Ser Pro 
        35                  40                  45              


Ile Thr Gln Gly Val Glu Thr Thr Lys Pro Ser Lys Gln Ser Asn Asn 
    50                  55                  60                  


Lys Tyr Ala Ala Ser Ser Tyr Leu Ser Leu Thr Pro Asp Lys Trp Lys 
65                  70                  75                  80  


Ser His Ser Ser Phe Ser Cys Leu Val Thr His Glu Gly Ser Thr Val 
                85                  90                  95      


Glu Lys Lys Val Ala Pro Ala Glu Cys Ser 
            100                 105     


<210>  53
<211>  331
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Canine cIgG1 heavy chain

<400>  53

Ala Ser Thr Thr Ala Pro Ser Val Phe Pro Leu Ala Pro Ser Cys Gly 
1               5                   10                  15      


Ser Thr Ser Gly Ser Thr Val Ala Leu Ala Cys Leu Val Ser Gly Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ser Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ser Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Met Val Thr Val Pro Ser Ser Arg Trp Pro Ser Glu Thr 
65                  70                  75                  80  


Phe Thr Cys Asn Val Val His Pro Ala Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Pro Val Phe Asn Glu Cys Arg Cys Thr Asp Thr Pro Pro Cys Pro Val 
            100                 105                 110         


Pro Glu Pro Leu Gly Gly Pro Ser Val Leu Ile Phe Pro Pro Lys Pro 
        115                 120                 125             


Lys Asp Ile Leu Arg Ile Thr Arg Thr Pro Glu Val Thr Cys Val Val 
    130                 135                 140                 


Leu Asp Leu Gly Arg Glu Asp Pro Glu Val Gln Ile Ser Trp Phe Val 
145                 150                 155                 160 


Asp Gly Lys Glu Val His Thr Ala Lys Thr Gln Ser Arg Glu Gln Gln 
                165                 170                 175     


Phe Asn Gly Thr Tyr Arg Val Val Ser Val Leu Pro Ile Glu His Gln 
            180                 185                 190         


Asp Trp Leu Thr Gly Lys Glu Phe Lys Cys Arg Val Asn His Ile Asp 
        195                 200                 205             


Leu Pro Ser Pro Ile Glu Arg Thr Ile Ser Lys Ala Arg Gly Arg Ala 
    210                 215                 220                 


His Lys Pro Ser Val Tyr Val Leu Pro Pro Ser Pro Lys Glu Leu Ser 
225                 230                 235                 240 


Ser Ser Asp Thr Val Ser Val Thr Cys Leu Ile Lys Asp Phe Tyr Pro 
                245                 250                 255     


Pro Asp Ile Asp Val Glu Trp Gln Ser Asn Gly Gln Gln Glu Pro Glu 
            260                 265                 270         


Arg Lys His Arg Met Thr Pro Pro Gln Leu Asp Glu Asp Gly Ser Tyr 
        275                 280                 285             


Phe Leu Tyr Ser Lys Leu Ser Val Asp Lys Ser Arg Trp Gln Gln Gly 
    290                 295                 300                 


Asp Pro Phe Thr Cys Ala Val Met His Glu Thr Leu Gln Asn His Tyr 
305                 310                 315                 320 


Thr Asp Leu Ser Leu Ser His Ser Pro Gly Lys 
                325                 330     


<210>  54
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cCTLA-4 For

<400>  54
cacgaactcg agaccatggc tggctttgga ttccggagg                              39


<210>  55
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cCTLA-4 Rev

<400>  55
atgcattcac tcgtgccact cgatcttctg ggcctcgaag atgtcgttca ggccgtgatg       60

gtgatggtga tggcttccgc cgcttccgcc gaagtcagaa tct                        103


<210>  56
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cCTLA-4 For

<400>  56
acgctgaatt catggctggc tttggattcc ggaggcat                               38


<210>  57
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cCTLA-4 Rev

<400>  57
acagtgtcga ctcaattgat gggaataaaa taa                                    33


<210>  58
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker


<220>
<221>  REPEAT
<222>  (1)..(5)
<223>  Repeat n times, where n is an integer of at least one.

<400>  58

Gly Ser Gly Gly Ser 
1               5   


<210>  59
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker


<220>
<221>  REPEAT
<222>  (1)..(4)
<223>  Repeat n times, where n is an integer of at least one.

<400>  59

Gly Gly Gly Ser 
1               


<210>  60
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker


<220>
<221>  REPEAT
<222>  (1)..(5)
<223>  Repeat n times, where n is an integer of at least one.

<400>  60

Gly Gly Gly Gly Ser 
1               5   


<210>  61
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  61

Gly Gly Ser Gly 
1               


<210>  62
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  62

Gly Gly Ser Gly Gly 
1               5   


<210>  63
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  63

Gly Ser Gly Ser Gly 
1               5   


<210>  64
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  64

Gly Ser Gly Gly Gly 
1               5   


<210>  65
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  65

Gly Gly Gly Ser Gly 
1               5   


<210>  66
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  66

Gly Ser Ser Ser Gly 
1               5   


<210>  67
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  67

Gly Gly Gly Gly Ser 
1               5   


<210>  68
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  68

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  69
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  69

Gly Gly Gly Ser Ser Arg Ser Ser Ser Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly 
            


<210>  70
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  70

Gly Gly Cys Gly Gly Thr Gly Gly Thr Thr Cys Cys Thr Cys Thr Ala 
1               5                   10                  15      


Gly Ala Thr Cys Thr Thr Cys Cys Thr Cys Cys Thr Cys Thr Gly Gly 
            20                  25                  30          


Thr Gly Gly Cys Gly Gly Thr Gly Gly Cys Thr Cys Gly Gly Gly Cys 
        35                  40                  45              


Gly Gly Thr Gly Gly Thr Gly Gly Gly 
    50                  55          


<210>  71
<211>  335
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Canine cIgG2 heavy chain

<400>  71

Ala Ser Thr Thr Ala Pro Ser Val Phe Pro Leu Ala Pro Ser Cys Gly 
1               5                   10                  15      


Ser Thr Ser Gly Ser Thr Val Ala Leu Ala Cys Leu Val Ser Gly Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ser Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ser Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Met Val Thr Val Pro Ser Ser Arg Trp Pro Ser Glu Thr 
65                  70                  75                  80  


Phe Thr Cys Asn Val Ala His Pro Ala Ser Lys Thr Lys Val Asp Lys 
                85                  90                  95      


Pro Val Pro Lys Arg Glu Asn Gly Arg Val Pro Arg Pro Pro Asp Cys 
            100                 105                 110         


Pro Lys Cys Pro Ala Pro Glu Met Leu Gly Gly Pro Ser Val Phe Ile 
        115                 120                 125             


Phe Pro Pro Lys Pro Lys Asp Thr Leu Leu Ile Ala Arg Thr Pro Glu 
    130                 135                 140                 


Val Thr Cys Val Val Val Asp Leu Asp Pro Glu Asp Pro Glu Val Gln 
145                 150                 155                 160 


Ile Ser Trp Phe Val Asp Gly Lys Gln Met Gln Thr Ala Lys Thr Gln 
                165                 170                 175     


Pro Arg Glu Glu Gln Phe Asn Gly Thr Tyr Arg Val Val Ser Val Leu 
            180                 185                 190         


Pro Ile Gly His Gln Asp Trp Leu Lys Gly Lys Gln Phe Thr Cys Lys 
        195                 200                 205             


Val Asn Asn Lys Ala Leu Pro Ser Pro Ile Glu Arg Thr Ile Ser Lys 
    210                 215                 220                 


Ala Arg Gly Gln Ala His Gln Pro Ser Val Tyr Val Leu Pro Pro Ser 
225                 230                 235                 240 


Arg Glu Glu Leu Ser Lys Asn Thr Val Ser Leu Thr Cys Leu Ile Lys 
                245                 250                 255     


Asp Phe Phe Pro Pro Asp Ile Asp Val Glu Trp Gln Ser Asn Gly Gln 
            260                 265                 270         


Gln Glu Pro Glu Ser Lys Tyr Arg Thr Thr Pro Pro Gln Leu Asp Glu 
        275                 280                 285             


Asp Gly Ser Tyr Phe Leu Tyr Ser Lys Leu Ser Val Asp Lys Ser Arg 
    290                 295                 300                 


Trp Gln Arg Gly Asp Thr Phe Ile Cys Ala Val Met His Glu Ala Leu 
305                 310                 315                 320 


His Asn His Tyr Thr Gln Glu Ser Leu Ser His Ser Pro Gly Lys 
                325                 330                 335 


<210>  72
<211>  333
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Canine cIgG3 heavy chain

<400>  72

Ala Ser Thr Thr Ala Pro Ser Val Phe Pro Leu Ala Pro Ser Cys Gly 
1               5                   10                  15      


Ser Gln Ser Gly Ser Thr Val Ala Leu Ala Cys Leu Val Ser Gly Tyr 
            20                  25                  30          


Ile Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ser Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ser Ile Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Met Val Thr Val Pro Ser Ser Arg Trp Pro Ser Glu Thr 
65                  70                  75                  80  


Phe Thr Cys Asn Val Ala His Pro Ala Thr Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Pro Val Val Lys Glu Cys Glu Cys Lys Cys Asn Cys Asn Asn Cys Pro 
            100                 105                 110         


Cys Pro Gly Cys Gly Leu Leu Gly Gly Pro Ser Val Phe Ile Phe Pro 
        115                 120                 125             


Pro Lys Pro Lys Asp Ile Leu Val Thr Ala Arg Thr Pro Thr Val Thr 
    130                 135                 140                 


Cys Val Val Val Asp Leu Asp Pro Glu Asn Pro Glu Val Gln Ile Ser 
145                 150                 155                 160 


Trp Phe Val Asp Ser Lys Gln Val Gln Thr Ala Asn Thr Gln Pro Arg 
                165                 170                 175     


Glu Glu Gln Ser Asn Gly Thr Tyr Arg Val Val Ser Val Leu Pro Ile 
            180                 185                 190         


Gly His Gln Asp Trp Leu Ser Gly Lys Gln Phe Lys Cys Lys Val Asn 
        195                 200                 205             


Asn Lys Ala Leu Pro Ser Pro Ile Glu Glu Ile Ile Ser Lys Thr Pro 
    210                 215                 220                 


Gly Gln Ala His Gln Pro Asn Val Tyr Val Leu Pro Pro Ser Arg Asp 
225                 230                 235                 240 


Glu Met Ser Lys Asn Thr Val Thr Leu Thr Cys Leu Val Lys Asp Phe 
                245                 250                 255     


Phe Pro Pro Glu Ile Asp Val Glu Trp Gln Ser Asn Gly Gln Gln Glu 
            260                 265                 270         


Pro Glu Ser Lys Tyr Arg Met Thr Pro Pro Gln Leu Asp Glu Asp Gly 
        275                 280                 285             


Ser Tyr Phe Leu Tyr Ser Lys Leu Ser Val Asp Lys Ser Arg Trp Gln 
    290                 295                 300                 


Arg Gly Asp Thr Phe Ile Cys Ala Val Met His Glu Ala Leu His Asn 
305                 310                 315                 320 


His Tyr Thr Gln Lys Ser Leu Ser His Ser Pro Gly Lys 
                325                 330             


<210>  73
<211>  331
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Canine cIgG4 heavy chain

<400>  73

Ala Ser Thr Thr Ala Pro Ser Val Phe Pro Leu Ala Pro Ser Cys Gly 
1               5                   10                  15      


Ser Thr Ser Gly Ser Thr Val Ala Leu Ala Cys Leu Val Ser Gly Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ser Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ser Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Met Val Thr Val Pro Ser Ser Arg Trp Pro Ser Glu Thr 
65                  70                  75                  80  


Phe Thr Cys Asn Val Val His Pro Ala Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Pro Val Pro Lys Glu Ser Thr Cys Lys Cys Ile Ser Pro Cys Pro Val 
            100                 105                 110         


Pro Glu Ser Leu Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro 
        115                 120                 125             


Lys Asp Ile Leu Arg Ile Thr Arg Thr Pro Glu Ile Thr Cys Val Val 
    130                 135                 140                 


Leu Asp Leu Gly Arg Glu Asp Pro Glu Val Gln Ile Ser Trp Phe Val 
145                 150                 155                 160 


Asp Gly Lys Glu Val His Thr Ala Lys Thr Gln Pro Arg Glu Gln Gln 
                165                 170                 175     


Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Glu His Gln 
            180                 185                 190         


Asp Trp Leu Thr Gly Lys Glu Phe Lys Cys Arg Val Asn His Ile Gly 
        195                 200                 205             


Leu Pro Ser Pro Ile Glu Arg Thr Ile Ser Lys Ala Arg Gly Gln Ala 
    210                 215                 220                 


His Gln Pro Ser Val Tyr Val Leu Pro Pro Ser Pro Lys Glu Leu Ser 
225                 230                 235                 240 


Ser Ser Asp Thr Val Thr Leu Thr Cys Leu Ile Lys Asp Phe Phe Pro 
                245                 250                 255     


Pro Glu Ile Asp Val Glu Trp Gln Ser Asn Gly Gln Pro Glu Pro Glu 
            260                 265                 270         


Ser Lys Tyr His Thr Thr Ala Pro Gln Leu Asp Glu Asp Gly Ser Tyr 
        275                 280                 285             


Phe Leu Tyr Ser Lys Leu Ser Val Asp Lys Ser Arg Trp Gln Gln Gly 
    290                 295                 300                 


Asp Pro Phe Thr Cys Ala Val Met His Glu Ala Leu Gln Asn His Tyr 
305                 310                 315                 320 


Thr Asp Leu Ser Leu Ser His Ser Pro Gly Lys 
                325                 330     


<210>  74
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1 VH PRT

<400>  74

Glu Val Gln Leu Val Glu Ser Gly Gly Asp Leu Val Lys Pro Ala Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Phe Ser Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Gln Trp Ile 
        35                  40                  45              


Ala Gly Ile Asn Ser Gly Gly Ser Ser Thr Ser His Ile Asp Ala Ile 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ile Ser Asn Trp Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val 
            100                 105                 110         


Ser Ser 
        


<210>  75
<211>  342
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A1 VH DNA

<400>  75
gaggtgcagc tggtggagtc tgggggagac ctggtgaagc ctgcagggtc cctgagattg       60

tcctgtgtgg cctctggatt ctccttcagc agttatgcca tgaactgggt ccgccaggct      120

cctgggaagg ggctgcagtg gatcgcaggt attaatagcg gtggaagtag tacaagtcat      180

atagacgcta taaagggccg cttcaccatc tccagagaca acgccaagaa cacactgtat      240

ttacagatga atagcctgag agccgaggac acggccgtgt attactgtgc gattagtaat      300

tgggcctact ggggccaggg aaccctggtc accgtctcct ca                         342


<210>  76
<211>  276
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1 scFv PRT

<400>  76

Gln Ala Val Leu Asn Gln Pro Ala Ser Val Ser Gly Ser Leu Gly Gln 
1               5                   10                  15      


Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asp Ile Gly Lys Ser 
            20                  25                  30          


Tyr Val Ala Trp Tyr Gln Gln Leu Pro Gly Thr Gly Pro Arg Thr Leu 
        35                  40                  45              


Ile Asn Val Asp Gly Asn Arg Ala Ser Gly Val Pro Asp Arg Phe Ser 
    50                  55                  60                  


Val Ser Arg Ser Gly Asn Thr Ala Thr Leu Thr Ile Ser Gly Leu Gln 
65                  70                  75                  80  


Ala Glu Asp Glu Ala Asp Tyr His Cys Ser Ser Trp Asp Trp Ser Leu 
                85                  90                  95      


His Thr Tyr Val Phe Gly Ser Gly Thr Gln Leu Thr Ile Leu Gly Gly 
            100                 105                 110         


Gly Ser Ser Arg Ser Ser Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Glu Val Gln Leu Val Glu Ser Gly Gly Asp Leu Val Lys Pro Ala 
    130                 135                 140                 


Gly Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Phe Ser Phe Ser Ser 
145                 150                 155                 160 


Tyr Ala Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Gln Trp 
                165                 170                 175     


Ile Ala Gly Ile Asn Ser Gly Gly Ser Ser Thr Ser His Ile Asp Ala 
            180                 185                 190         


Ile Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu 
        195                 200                 205             


Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr 
    210                 215                 220                 


Cys Ala Ile Ser Asn Trp Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr 
225                 230                 235                 240 


Val Ser Ser Glu Ser Pro Ser Pro Pro Asn Leu Thr Ser Gly Gln Ala 
                245                 250                 255     


Gly Gln His His His His His His Gly Ala Tyr Pro Tyr Asp Val Pro 
            260                 265                 270         


Asp Tyr Ala Ser 
        275     


<210>  77
<211>  994
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A1 scFv DNA

<400>  77
caggctgtgc tgaatcagcc ggcctcagtg tccgggtccc tgggccagag ggtcaccatc       60

tcctgcactg gaagcagctc cgacatcggt aaaagttatg tggcctggta ccagcagctc      120

ccgggaacag gccccagaac cctcatcaat gttgatggta accgagcctc aggggtcccc      180

gatcgattct ctgtctccag gtcaggcaac acagccaccc tgaccatctc cgggctccag      240

gctgaggatg aggctgatta tcactgctca tcctgggact ggagtctcca tacttacgtg      300

ttcggctcag ggacccagct gaccatcctc ggtggtggtt cctctagatc ttcctcctct      360

ggtggcggtg gctcgggcgg tggtggggag gtgcagctgg tggagtctgg gggagacctg      420

gtgaagcctg cagggtccct gagattgtcc tgtgtggcct ctggattctc cttcagcagt      480

tatgccatga actgggtccg ccaggctcct gggaaggggc tgcagtggat cgcaggtatt      540

aatagcggtg gaagtagtac aagtcatata gacgctataa agggccgctt caccatctcc      600

agagacaacg ccaagaacac actgtattta cagatgaata gcctgagagc cgaggacacg      660

gccgtgtatt actgtgcgat tagtaattgg gcctactggg gccagggaac cctggtcacc      720

gtctcctcag agagtccatc ccctcccaac ctcactagtg gccaggccgg ccagcaccat      780

caccatcacc atggcgcata cccgtacgac gttccggact acgcttctta ggagggtggt      840

ggctctgagg gtggcggttc tgagggtggc ggctctgagg gaggcggttc cggtggtggc      900

tctggttccg gtgattttga ttatgaaaag atggcaaacg ctaataaggg gctatgaccg      960

aaaatgccga tgaaaacgtg ctacagtctg acgc                                  994


