                         SEQUENCE LISTING

<110>  BioLegend, Inc
       TBJK GmbH
 
<120>  PHOSPHATIDYLSERINE BINDING AGENTS FOR THE DETECTION AND DEPLETION
        OF PHOSPHATIDYLSERINE POSITIVE CELLS

<130>  BLD021PCT

<150>  63/178,427
<151>  2021-04-22

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  156
<212>  PRT
<213>  mus musculus


<220>
<221>  MISC_FEATURE
<223>  murine C1 domain

<400>  1

Cys Ser Thr Gln Leu Gly Met Glu Gly Gly Ala Ile Ala Asp Ser Gln 
1               5                   10                  15      


Ile Ser Ala Ser Ser Val Tyr Met Gly Phe Met Gly Leu Gln Arg Trp 
            20                  25                  30          


Gly Pro Glu Leu Ala Arg Leu Tyr Arg Thr Gly Ile Val Asn Ala Trp 
        35                  40                  45              


Thr Ala Ser Asn Tyr Asp Ser Lys Pro Trp Ile Gln Val Asn Leu Leu 
    50                  55                  60                  


Arg Lys Met Arg Val Ser Gly Val Met Thr Gln Gly Ala Ser Arg Ala 
65                  70                  75                  80  


Gly Arg Ala Glu Tyr Leu Lys Thr Phe Lys Val Ala Tyr Ser Leu Asp 
                85                  90                  95      


Gly Arg Lys Phe Glu Phe Ile Gln Asp Glu Ser Gly Gly Asp Lys Glu 
            100                 105                 110         


Phe Leu Gly Asn Leu Asp Asn Asn Ser Leu Lys Val Asn Met Phe Asn 
        115                 120                 125             


Pro Thr Leu Glu Ala Gln Tyr Ile Lys Leu Tyr Pro Val Ser Cys His 
    130                 135                 140                 


Arg Gly Cys Thr Leu Arg Phe Glu Leu Leu Gly Cys 
145                 150                 155     


<210>  2
<211>  156
<212>  PRT
<213>  mus musculus


<220>
<221>  MISC_FEATURE
<223>  murine C2 domain

<400>  2

Cys Ser Glu Pro Leu Gly Leu Lys Asn Asn Thr Ile Pro Asp Ser Gln 
1               5                   10                  15      


Met Ser Ala Ser Ser Ser Tyr Lys Thr Trp Asn Leu Arg Ala Phe Gly 
            20                  25                  30          


Trp Tyr Pro His Leu Gly Arg Leu Asp Asn Gln Gly Lys Ile Asn Ala 
        35                  40                  45              


Trp Thr Ala Gln Ser Asn Ser Ala Lys Glu Trp Leu Gln Val Asp Leu 
    50                  55                  60                  


Gly Thr Gln Arg Gln Val Thr Gly Ile Ile Thr Gln Gly Ala Arg Asp 
65                  70                  75                  80  


Phe Gly His Ile Gln Tyr Val Ala Ser Tyr Lys Val Ala His Ser Asp 
                85                  90                  95      


Asp Gly Val Gln Trp Thr Val Tyr Glu Glu Gln Gly Ser Ser Lys Val 
            100                 105                 110         


Phe Gln Gly Asn Leu Asp Asn Asn Ser His Lys Lys Asn Ile Phe Glu 
        115                 120                 125             


Lys Pro Phe Met Ala Arg Tyr Val Arg Val Leu Pro Val Ser Trp His 
    130                 135                 140                 


Asn Arg Ile Thr Leu Arg Leu Glu Leu Leu Gly Cys 
145                 150                 155     


<210>  3
<211>  387
<212>  PRT
<213>  homo sapiens


<220>
<221>  MISC_FEATURE
<223>  full length human MFG-E8

<400>  3

Met Pro Arg Pro Arg Leu Leu Ala Ala Leu Cys Gly Ala Leu Leu Cys 
1               5                   10                  15      


Ala Pro Ser Leu Leu Val Ala Leu Asp Ile Cys Ser Lys Asn Pro Cys 
            20                  25                  30          


His Asn Gly Gly Leu Cys Glu Glu Ile Ser Gln Glu Val Arg Gly Asp 
        35                  40                  45              


Val Phe Pro Ser Tyr Thr Cys Thr Cys Leu Lys Gly Tyr Ala Gly Asn 
    50                  55                  60                  


His Cys Glu Thr Lys Cys Val Glu Pro Leu Gly Leu Glu Asn Gly Asn 
65                  70                  75                  80  


Ile Ala Asn Ser Gln Ile Ala Ala Ser Ser Val Arg Val Thr Phe Leu 
                85                  90                  95      


Gly Leu Gln His Trp Val Pro Glu Leu Ala Arg Leu Asn Arg Ala Gly 
            100                 105                 110         


Met Val Asn Ala Trp Thr Pro Ser Ser Asn Asp Asp Asn Pro Trp Ile 
        115                 120                 125             


Gln Val Asn Leu Leu Arg Arg Met Trp Val Thr Gly Val Val Thr Gln 
    130                 135                 140                 


Gly Ala Ser Arg Leu Ala Ser His Glu Tyr Leu Lys Ala Phe Lys Val 
145                 150                 155                 160 


Ala Tyr Ser Leu Asn Gly His Glu Phe Asp Phe Ile His Asp Val Asn 
                165                 170                 175     


Lys Lys His Lys Glu Phe Val Gly Asn Trp Asn Lys Asn Ala Val His 
            180                 185                 190         


Val Asn Leu Phe Glu Thr Pro Val Glu Ala Gln Tyr Val Arg Leu Tyr 
        195                 200                 205             


Pro Thr Ser Cys His Thr Ala Cys Thr Leu Arg Phe Glu Leu Leu Gly 
    210                 215                 220                 


Cys Glu Leu Asn Gly Cys Ala Asn Pro Leu Gly Leu Lys Asn Asn Ser 
225                 230                 235                 240 


Ile Pro Asp Lys Gln Ile Thr Ala Ser Ser Ser Tyr Lys Thr Trp Gly 
                245                 250                 255     


Leu His Leu Phe Ser Trp Asn Pro Ser Tyr Ala Arg Leu Asp Lys Gln 
            260                 265                 270         


Gly Asn Phe Asn Ala Trp Val Ala Gly Ser Tyr Gly Asn Asp Gln Trp 
        275                 280                 285             


Leu Gln Val Asp Leu Gly Ser Ser Lys Glu Val Thr Gly Ile Ile Thr 
    290                 295                 300                 


Gln Gly Ala Arg Asn Phe Gly Ser Val Gln Phe Val Ala Ser Tyr Lys 
305                 310                 315                 320 


Val Ala Tyr Ser Asn Asp Ser Ala Asn Trp Thr Glu Tyr Gln Asp Pro 
                325                 330                 335     


Arg Thr Gly Ser Ser Lys Ile Phe Pro Gly Asn Trp Asp Asn His Ser 
            340                 345                 350         


His Lys Lys Asn Leu Phe Glu Thr Pro Ile Leu Ala Arg Tyr Val Arg 
        355                 360                 365             


Ile Leu Pro Val Ala Trp His Asn Arg Ile Ala Leu Arg Leu Glu Leu 
    370                 375                 380                 


Leu Gly Cys 
385         


<210>  4
<211>  463
<212>  PRT
<213>  mus musculus


<220>
<221>  MISC_FEATURE
<223>  full length mouse MFG-E8 protein

<400>  4

Met Gln Val Ser Arg Val Leu Ala Ala Leu Cys Gly Met Leu Leu Cys 
1               5                   10                  15      


Ala Ser Gly Leu Phe Ala Ala Ser Gly Asp Phe Cys Asp Ser Ser Leu 
            20                  25                  30          


Cys Leu Asn Gly Gly Thr Cys Leu Thr Gly Gln Asp Asn Asp Ile Tyr 
        35                  40                  45              


Cys Leu Cys Pro Glu Gly Phe Thr Gly Leu Val Cys Asn Glu Thr Glu 
    50                  55                  60                  


Arg Gly Pro Cys Ser Pro Asn Pro Cys Tyr Asn Asp Ala Lys Cys Leu 
65                  70                  75                  80  


Val Thr Leu Asp Thr Gln Arg Gly Asp Ile Phe Thr Glu Tyr Ile Cys 
                85                  90                  95      


Gln Cys Pro Val Gly Tyr Ser Gly Ile His Cys Glu Thr Glu Thr Asn 
            100                 105                 110         


Tyr Tyr Asn Leu Asp Gly Glu Tyr Met Phe Thr Thr Ala Val Pro Asn 
        115                 120                 125             


Thr Ala Val Pro Thr Pro Ala Pro Thr Pro Asp Leu Ser Asn Asn Leu 
    130                 135                 140                 


Ala Ser Arg Cys Ser Thr Gln Leu Gly Met Glu Gly Gly Ala Ile Ala 
145                 150                 155                 160 


Asp Ser Gln Ile Ser Ala Ser Ser Val Tyr Met Gly Phe Met Gly Leu 
                165                 170                 175     


Gln Arg Trp Gly Pro Glu Leu Ala Arg Leu Tyr Arg Thr Gly Ile Val 
            180                 185                 190         


Asn Ala Trp Thr Ala Ser Asn Tyr Asp Ser Lys Pro Trp Ile Gln Val 
        195                 200                 205             


Asn Leu Leu Arg Lys Met Arg Val Ser Gly Val Met Thr Gln Gly Ala 
    210                 215                 220                 


Ser Arg Ala Gly Arg Ala Glu Tyr Leu Lys Thr Phe Lys Val Ala Tyr 
225                 230                 235                 240 


Ser Leu Asp Gly Arg Lys Phe Glu Phe Ile Gln Asp Glu Ser Gly Gly 
                245                 250                 255     


Asp Lys Glu Phe Leu Gly Asn Leu Asp Asn Asn Ser Leu Lys Val Asn 
            260                 265                 270         


Met Phe Asn Pro Thr Leu Glu Ala Gln Tyr Ile Lys Leu Tyr Pro Val 
        275                 280                 285             


Ser Cys His Arg Gly Cys Thr Leu Arg Phe Glu Leu Leu Gly Cys Glu 
    290                 295                 300                 


Leu His Gly Cys Ser Glu Pro Leu Gly Leu Lys Asn Asn Thr Ile Pro 
305                 310                 315                 320 


Asp Ser Gln Met Ser Ala Ser Ser Ser Tyr Lys Thr Trp Asn Leu Arg 
                325                 330                 335     


Ala Phe Gly Trp Tyr Pro His Leu Gly Arg Leu Asp Asn Gln Gly Lys 
            340                 345                 350         


Ile Asn Ala Trp Thr Ala Gln Ser Asn Ser Ala Lys Glu Trp Leu Gln 
        355                 360                 365             


Val Asp Leu Gly Thr Gln Arg Gln Val Thr Gly Ile Ile Thr Gln Gly 
    370                 375                 380                 


Ala Arg Asp Phe Gly His Ile Gln Tyr Val Ala Ser Tyr Lys Val Ala 
385                 390                 395                 400 


His Ser Asp Asp Gly Val Gln Trp Thr Val Tyr Glu Glu Gln Gly Ser 
                405                 410                 415     


Ser Lys Val Phe Gln Gly Asn Leu Asp Asn Asn Ser His Lys Lys Asn 
            420                 425                 430         


Ile Phe Glu Lys Pro Phe Met Ala Arg Tyr Val Arg Val Leu Pro Val 
        435                 440                 445             


Ser Trp His Asn Arg Ile Thr Leu Arg Leu Glu Leu Leu Gly Cys 
    450                 455                 460             


<210>  5
<211>  316
<212>  PRT
<213>  mus musculus


<220>
<221>  MISC_FEATURE
<223>  mouse PS-binding domain (C1+C2 domain)

<400>  5

Cys Ser Thr Gln Leu Gly Met Glu Gly Gly Ala Ile Ala Asp Ser Gln 
1               5                   10                  15      


Ile Ser Ala Ser Ser Val Tyr Met Gly Phe Met Gly Leu Gln Arg Trp 
            20                  25                  30          


Gly Pro Glu Leu Ala Arg Leu Tyr Arg Thr Gly Ile Val Asn Ala Trp 
        35                  40                  45              


Thr Ala Ser Asn Tyr Asp Ser Lys Pro Trp Ile Gln Val Asn Leu Leu 
    50                  55                  60                  


Arg Lys Met Arg Val Ser Gly Val Met Thr Gln Gly Ala Ser Arg Ala 
65                  70                  75                  80  


Gly Arg Ala Glu Tyr Leu Lys Thr Phe Lys Val Ala Tyr Ser Leu Asp 
                85                  90                  95      


Gly Arg Lys Phe Glu Phe Ile Gln Asp Glu Ser Gly Gly Asp Lys Glu 
            100                 105                 110         


Phe Leu Gly Asn Leu Asp Asn Asn Ser Leu Lys Val Asn Met Phe Asn 
        115                 120                 125             


Pro Thr Leu Glu Ala Gln Tyr Ile Lys Leu Tyr Pro Val Ser Cys His 
    130                 135                 140                 


Arg Gly Cys Thr Leu Arg Phe Glu Leu Leu Gly Cys Glu Leu His Gly 
145                 150                 155                 160 


Cys Ser Glu Pro Leu Gly Leu Lys Asn Asn Thr Ile Pro Asp Ser Gln 
                165                 170                 175     


Met Ser Ala Ser Ser Ser Tyr Lys Thr Trp Asn Leu Arg Ala Phe Gly 
            180                 185                 190         


Trp Tyr Pro His Leu Gly Arg Leu Asp Asn Gln Gly Lys Ile Asn Ala 
        195                 200                 205             


Trp Thr Ala Gln Ser Asn Ser Ala Lys Glu Trp Leu Gln Val Asp Leu 
    210                 215                 220                 


Gly Thr Gln Arg Gln Val Thr Gly Ile Ile Thr Gln Gly Ala Arg Asp 
225                 230                 235                 240 


Phe Gly His Ile Gln Tyr Val Ala Ser Tyr Lys Val Ala His Ser Asp 
                245                 250                 255     


Asp Gly Val Gln Trp Thr Val Tyr Glu Glu Gln Gly Ser Ser Lys Val 
            260                 265                 270         


Phe Gln Gly Asn Leu Asp Asn Asn Ser His Lys Lys Asn Ile Phe Glu 
        275                 280                 285             


Lys Pro Phe Met Ala Arg Tyr Val Arg Val Leu Pro Val Ser Trp His 
    290                 295                 300                 


Asn Arg Ile Thr Leu Arg Leu Glu Leu Leu Gly Cys 
305                 310                 315     


<210>  6
<211>  12
<212>  PRT
<213>  artificial sequence

<220>
<223>  linker

<400>  6

Gly Ser Ala Gly Ser Ala Ala Gly Ser Gly Glu Phe 
1               5                   10          


<210>  7
<211>  5
<212>  PRT
<213>  artificial sequence

<220>
<223>  sortase A recognition sequence


<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  Xaa is any amino acid

<400>  7

Leu Pro Xaa Thr Gly 
1               5   


<210>  8
<211>  5
<212>  PRT
<213>  artificial sequence

<220>
<223>  sortase A recognition sequence


<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  Xaa is any amino acid

<400>  8

Leu Pro Xaa Thr Ala 
1               5   


<210>  9
<211>  8
<212>  PRT
<213>  artificial sequence

<220>
<223>  sortase B recognition sequence


<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  Xaa is any amino acid

<400>  9

Asp Xaa Pro Asn Thr Gly Asp Glu 
1               5               


<210>  10
<211>  28
<212>  DNA
<213>  artificial sequence

<220>
<223>  Priming sequence at 5' end

<400>  10
tgtttaactt taagaaggag atatacat                                          28


<210>  11
<211>  31
<212>  DNA
<213>  artificial sequence

<220>
<223>  Priming sequence at 3' end

<400>  11
taactcgagc accaccacca ccaccactga g                                      31


<210>  12
<211>  11
<212>  PRT
<213>  artificial sequence

<220>
<223>  Sequence at 3'-end of C1 and C2 fragments in cloning

<400>  12

Gly Ser Leu Pro Glu Thr Gly Gly Ser Leu Glu 
1               5                   10      


<210>  13
<211>  5
<212>  PRT
<213>  artificial sequence

<220>
<223>  Sortase recognition sequence

<400>  13

Leu Pro Glu Thr Gly 
1               5   


