<110>    Ewha University - Industry Collaboration Foundation

<120>    Multifunctional nucleic acid construct for target gene regulation
         and uses thereof

<130>    OPP20220423KR

<150>    10-2021-0052010
<151>    2021-04-21

<160>    47

<170>    koPatentIn 3.0

<210>    1
<211>    21
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_siGFP_Sense


<220>
<221>    misc_feature
<222>    (20)..(21)
<223>    deoxyribonucleotides


<400>    1
gcaagcugac ccugaaguut t                                                   21


<210>    2
<211>    21
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_siGFP_Antisense


<220>
<221>    misc_feature
<222>    (20)..(21)
<223>    deoxyribonucleotides


<400>    2
aacuucaggg ucagcuugct t                                                   21


<210>    3
<211>    21
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_siHPRT_Sense


<220>
<221>    misc_feature
<222>    (20)..(21)
<223>    deoxyribonucleotides


<400>    3
cagacuuugu uggauuugat t                                                   21


<210>    4
<211>    21
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_siHPRT_Antisense


<220>
<221>    misc_feature
<222>    (20)..(21)
<223>    deoxyribonucleotides


<400>    4
ucaaauccaa caaagucugt t                                                   21


<210>    5
<211>    25
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_Dicer-substrate siGFP_Sense


<400>    5
gcaagcugac ccugaaguua ucacc                                               25


<210>    6
<211>    27
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_Dicer-substrate siGFP_Antisense


<400>    6
ggugauaacu ucagggucag cuugcca                                             27


<210>    7
<211>    80
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_pri-miLet7B_5' fragment


<400>    7
caaggccggg ccuggcgggg ugagguagua gguugugugg uuucagggca gugauguugc         60

cccucggaag auaacuauac                                                     80


<210>    8
<211>    33
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_pri-miLet7B_3' fragment


<400>    8
aaccuacugc cuucccugag gagcccagug aca                                      33


<210>    9
<211>    78
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_pri-miGFP_5' fragment


<400>    9
ccauucccag cccuugcgcu acuccacggc aagcugaccc ugaaguucau guggacaggu         60

aaggagauga acuucagg                                                       78


<210>    10
<211>    38
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_pri-miGFP_3' fragment


<400>    10
gucagcuugc cguggcguag cgcucaaucc cuauagug                                 38


<210>    11
<211>    79
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_pri-miHPRT_5' fragment


<400>    11
ccauucccag cccuugcgcu acuccaugcc agacuuuguu ggauuugaau guggacaggu         60

aaggagauuc aaauccaac                                                      79


<210>    12
<211>    37
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_pri-miHPRT_3' fragment


<400>    12
aaagucuggc auggcguagc gcucaauccc uauagug                                  37


<210>    13
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_Primer_HPRT1 FW


<400>    13
gactttgctt tccttggtca g                                                   21


<210>    14
<211>    24
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_Primer_HPRT1 RV


<400>    14
ggcttatatc caacacttcg tggg                                                24


<210>    15
<211>    18
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_Primer_GAPDH FW


<400>    15
ggatttggtc gtattggg                                                       18


<210>    16
<211>    19
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_Primer_GAPDH RV


<400>    16
ggaagatggt gatgggatt                                                      19


<210>    17
<211>    78
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_Non-Mod_5' fragment


<400>    17
ccauucccag cccuugcgcu acuccacggc aagcugaccc ugaaguucau guggacaggu         60

aaggagauga acuucagg                                                       78


<210>    18
<211>    38
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_Non-Mod_3' fragment


<400>    18
gucagcuugc cguggcguag cgcucaaucc cuauagug                                 38


<210>    19
<211>    78
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_SS-Mod_5' fragment


<220>
<221>    modified_base
<222>    (1)..(3)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (6)..(9)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (11)..(13)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (55)..(57)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (61)..(62)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (65)
<223>    2'-OMe


<400>    19
ccauucccag cccuugcgcu acuccacggc aagcugaccc ugaaguucau guggacaggu         60

aaggagauga acuucagg                                                       78


<210>    20
<211>    38
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_SS-Mod_3' fragment


<220>
<221>    modified_base
<222>    (25)..(27)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (29)..(31)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (33)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (35)
<223>    2'-OMe


<400>    20
gucagcuugc cguggcguag cgcucaaucc cuauagug                                 38


<210>    21
<211>    78
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_ST-Mod_5' fragment


<220>
<221>    modified_base
<222>    (17)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (19)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (21)..(22)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (24)..(27)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (30)..(32)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (34)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (37)..(40)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (43)..(44)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (48)..(49)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (67)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (70)..(72)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (75)..(76)
<223>    2'-OMe


<400>    21
ccauucccag cccuugcgcu acuccacggc aagcugaccc ugaaguucau guggacaggu         60

aaggagauga acuucagg                                                       78


<210>    22
<211>    38
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_ST-Mod_3' fragment


<220>
<221>    modified_base
<222>    (3)..(4)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (6)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (10)..(11)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (16)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (19)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (21)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (23)
<223>    2'-OMe


<400>    22
gucagcuugc cguggcguag cgcucaaucc cuauagug                                 38


<210>    23
<211>    78
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_Seq-Mod_5' fragment


<220>
<221>    modified_base
<222>    (1)..(3)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (6)..(9)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (11)..(13)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (17)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (19)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (21)..(22)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (24)..(27)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (30)..(32)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (34)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (37)..(40)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (43)..(44)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (48)..(49)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (55)..(57)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (61)..(62)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (65)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (67)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (70)..(72)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (75)..(76)
<223>    2'-OMe


<400>    23
ccauucccag cccuugcgcu acuccacggc aagcugaccc ugaaguucau guggacaggu         60

aaggagauga acuucagg                                                       78


<210>    24
<211>    38
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_Seq-Mod_3' fragment


<220>
<221>    modified_base
<222>    (3)..(4)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (6)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (10)..(11)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (16)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (19)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (21)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (23)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (25)..(27)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (29)..(31)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (33)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (35)
<223>    2'-OMe


<400>    24
gucagcuugc cguggcguag cgcucaaucc cuauagug                                 38


<210>    25
<211>    78
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_PP-Mod_5' fragment


<220>
<221>    modified_base
<222>    (1)..(3)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (6)..(9)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (11)..(13)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (29)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (32)..(33)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (35)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (42)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (55)..(57)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (61)..(62)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (65)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (76)..(77)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (78)
<223>    2'-OMe


<400>    25
ccauucccag cccuugcgcu acuccacggc aagcugaccc ugaaguucau guggacaggu         60

aaggagauga acuucagg                                                       78


<210>    26
<211>    38
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_PP-Mod_3' fragment


<220>
<221>    modified_base
<222>    (1)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (3)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (5)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (8)..(9)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (25)..(27)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (29)..(31)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (33)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (35)
<223>    2'-OMe


<400>    26
gucagcuugc cguggcguag cgcucaaucc cuauagug                                 38


<210>    27
<211>    78
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_PP(-1)-Mod_5' fragment


<220>
<221>    modified_base
<222>    (1)..(3)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (6)..(9)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (11)..(13)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (32)..(33)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (35)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (42)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (55)..(57)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (61)..(62)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (65)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (76)..(77)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (78)
<223>    2'-OMe


<400>    27
ccauucccag cccuugcgcu acuccacggc aagcugaccc ugaaguucau guggacaggu         60

aaggagauga acuucagg                                                       78


<210>    28
<211>    38
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_PP(-1)-Mod_3' fragment


<220>
<221>    modified_base
<222>    (1)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (3)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (5)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (8)..(9)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (25)..(27)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (29)..(31)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (33)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (35)
<223>    2'-OMe


<400>    28
gucagcuugc cguggcguag cgcucaaucc cuauagug                                 38


<210>    29
<211>    78
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_PP(-2)-Mod_5' fragment


<220>
<221>    modified_base
<222>    (1)..(3)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (6)..(9)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (11)..(13)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (32)..(33)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (35)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (42)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (55)..(57)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (61)..(62)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (65)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (76)..(77)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (78)
<223>    2'-OMe


<400>    29
ccauucccag cccuugcgcu acuccacggc aagcugaccc ugaaguucau guggacaggu         60

aaggagauga acuucagg                                                       78


<210>    30
<211>    38
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_PP(-2)-Mod_3' fragment


<220>
<221>    modified_base
<222>    (1)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (3)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (5)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (8)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (25)..(27)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (29)..(31)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (33)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (35)
<223>    2'-OMe


<400>    30
gucagcuugc cguggcguag cgcucaaucc cuauagug                                 38


<210>    31
<211>    78
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_PP(-3)-Mod_5' fragment


<220>
<221>    modified_base
<222>    (1)..(3)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (6)..(9)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (11)..(13)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (32)..(33)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (35)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (42)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (55)..(57)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (61)..(62)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (65)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (76)..(77)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (78)
<223>    2'-OMe


<400>    31
ccauucccag cccuugcgcu acuccacggc aagcugaccc ugaaguucau guggacaggu         60

aaggagauga acuucagg                                                       78


<210>    32
<211>    38
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_PP(-3)-Mod_3' fragment


<220>
<221>    modified_base
<222>    (1)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (3)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (5)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (25)..(27)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (29)..(31)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (33)
<223>    2'-OMe


<220>
<221>    modified_base
<222>    (35)
<223>    2'-OMe


<400>    32
gucagcuugc cguggcguag cgcucaaucc cuauagug                                 38


<210>    33
<211>    42
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_3' SMN2-ASO1


<220>
<221>    misc_feature
<222>    (26)..(27)
<223>    Ribose is substituted for LNA(Locked nucleic acid)


<220>
<221>    misc_feature
<222>    (29)..(30)
<223>    Ribose is substituted for LNA(Locked nucleic acid)


<220>
<221>    misc_feature
<222>    (32)..(33)
<223>    Ribose is substituted for LNA(Locked nucleic acid)


<220>
<221>    misc_feature
<222>    (35)..(36)
<223>    Ribose is substituted for LNA(Locked nucleic acid)


<220>
<221>    misc_feature
<222>    (38)..(39)
<223>    Ribose is substituted for LNA(Locked nucleic acid)


<220>
<221>    misc_feature
<222>    (41)..(42)
<223>    Ribose is substituted for LNA(Locked nucleic acid)


<220>
<221>    misc_feature
<222>    (25)..(42)
<223>    phosphodiester bond is substituted for phosphorothioate bond


<400>    33
aaagucuggc auggcguagc gcuctcactt tcataatgct gg                            42


<210>    34
<211>    47
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_3' SMN2-ASO2


<220>
<221>    misc_feature
<222>    (31)..(32)
<223>    Ribose is substituted for LNA(Locked nucleic acid)


<220>
<221>    misc_feature
<222>    (34)..(35)
<223>    Ribose is substituted for LNA(Locked nucleic acid)


<220>
<221>    misc_feature
<222>    (38)..(39)
<223>    Ribose is substituted for LNA(Locked nucleic acid)


<220>
<221>    misc_feature
<222>    (40)..(41)
<223>    Ribose is substituted for LNA(Locked nucleic acid)


<220>
<221>    misc_feature
<222>    (43)..(44)
<223>    Ribose is substituted for LNA(Locked nucleic acid)


<220>
<221>    misc_feature
<222>    (46)..(47)
<223>    Ribose is substituted for LNA(Locked nucleic acid)


<220>
<221>    misc_feature
<222>    (30)..(47)
<223>    phosphodiester bond is substituted for phosphorothioate bond


<400>    34
aaagucuggc auggcguagc gcucaaucct cactttcata atgctgg                       47


<210>    35
<211>    22
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_Primer_SMN2 ?7 FW


<400>    35
tctggaccac caataattcc cc                                                  22


<210>    36
<211>    25
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_Primer_SMN2 ?7 RV


<400>    36
atgccagcat ttccatataa tagcc                                               25


<210>    37
<211>    29
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_Primer_SMN2 full FW


<400>    37
gctatcatac tggctattat atgggtttt                                           29


<210>    38
<211>    25
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_Primer_SMN2 full RV


<400>    38
ctctatgcca gcatttctcc ttaat                                               25


<210>    39
<211>    19
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_Primer_SMN2 E5F


<400>    39
ctgcctccat ttccttctg                                                      19


<210>    40
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_Primer_SMN2 E8R


<400>    40
tggtgtcatt tagtgctgct c                                                   21


<210>    41
<211>    37
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_3' Anti-miR21


<220>
<221>    misc_feature
<222>    (30)..(37)
<223>    Ribose is substituted for LNA(Locked nucleic acid)


<400>    41
aaagucuggc auggcguagc gcucaauccg ataagct                                  37


<210>    42
<211>    50
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_miR21 Stem-Loop


<400>    42
gtcgtatcca gtgcagggtc cgaggtattc gcactggata cgactcaaca                    50


<210>    43
<211>    25
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_miR21 FW


<400>    43
cggcggtagc ttatcagact gatgt                                               25


<210>    44
<211>    16
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_miR21 RV


<400>    44
gtgcagggtc cgaggt                                                         16


<210>    45
<211>    23
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_U6 Stem-Loop


<400>    45
cgcttcacga atttgcgtgt cat                                                 23


<210>    46
<211>    17
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_miR21 FW


<400>    46
ctcgcttcgg cagcaca                                                        17


<210>    47
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    synthetic_miR21 RV


<400>    47
aacgcttcac gaatttgcgt                                                     20
