                         SEQUENCE LISTING

<110>  Imperial College Innovations Limited
 
<120>  Chimeric antigen receptor (CAR)-T cells

<130>  115551PCT1

<150>  2105684.1
<151>  2021-04-21

<160>  36    

<170>  PatentIn version 3.5

<210>  1
<211>  458
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Asn Arg Gly Val Pro Phe Arg His Leu Leu Leu Val Leu Gln Leu 
1               5                   10                  15      


Ala Leu Leu Pro Ala Ala Thr Gln Gly Lys Lys Val Val Leu Gly Lys 
            20                  25                  30          


Lys Gly Asp Thr Val Glu Leu Thr Cys Thr Ala Ser Gln Lys Lys Ser 
        35                  40                  45              


Ile Gln Phe His Trp Lys Asn Ser Asn Gln Ile Lys Ile Leu Gly Asn 
    50                  55                  60                  


Gln Gly Ser Phe Leu Thr Lys Gly Pro Ser Lys Leu Asn Asp Arg Ala 
65                  70                  75                  80  


Asp Ser Arg Arg Ser Leu Trp Asp Gln Gly Asn Phe Pro Leu Ile Ile 
                85                  90                  95      


Lys Asn Leu Lys Ile Glu Asp Ser Asp Thr Tyr Ile Cys Glu Val Glu 
            100                 105                 110         


Asp Gln Lys Glu Glu Val Gln Leu Leu Val Phe Gly Leu Thr Ala Asn 
        115                 120                 125             


Ser Asp Thr His Leu Leu Gln Gly Gln Ser Leu Thr Leu Thr Leu Glu 
    130                 135                 140                 


Ser Pro Pro Gly Ser Ser Pro Ser Val Gln Cys Arg Ser Pro Arg Gly 
145                 150                 155                 160 


Lys Asn Ile Gln Gly Gly Lys Thr Leu Ser Val Ser Gln Leu Glu Leu 
                165                 170                 175     


Gln Asp Ser Gly Thr Trp Thr Cys Thr Val Leu Gln Asn Gln Lys Lys 
            180                 185                 190         


Val Glu Phe Lys Ile Asp Ile Val Val Leu Ala Phe Gln Lys Ala Ser 
        195                 200                 205             


Ser Ile Val Tyr Lys Lys Glu Gly Glu Gln Val Glu Phe Ser Phe Pro 
    210                 215                 220                 


Leu Ala Phe Thr Val Glu Lys Leu Thr Gly Ser Gly Glu Leu Trp Trp 
225                 230                 235                 240 


Gln Ala Glu Arg Ala Ser Ser Ser Lys Ser Trp Ile Thr Phe Asp Leu 
                245                 250                 255     


Lys Asn Lys Glu Val Ser Val Lys Arg Val Thr Gln Asp Pro Lys Leu 
            260                 265                 270         


Gln Met Gly Lys Lys Leu Pro Leu His Leu Thr Leu Pro Gln Ala Leu 
        275                 280                 285             


Pro Gln Tyr Ala Gly Ser Gly Asn Leu Thr Leu Ala Leu Glu Ala Lys 
    290                 295                 300                 


Thr Gly Lys Leu His Gln Glu Val Asn Leu Val Val Met Arg Ala Thr 
305                 310                 315                 320 


Gln Leu Gln Lys Asn Leu Thr Cys Glu Val Trp Gly Pro Thr Ser Pro 
                325                 330                 335     


Lys Leu Met Leu Ser Leu Lys Leu Glu Asn Lys Glu Ala Lys Val Ser 
            340                 345                 350         


Lys Arg Glu Lys Ala Val Trp Val Leu Asn Pro Glu Ala Gly Met Trp 
        355                 360                 365             


Gln Cys Leu Leu Ser Asp Ser Gly Gln Val Leu Leu Glu Ser Asn Ile 
    370                 375                 380                 


Lys Val Leu Pro Thr Trp Ser Thr Pro Val Gln Pro Met Ala Leu Ile 
385                 390                 395                 400 


Val Leu Gly Gly Val Ala Gly Leu Leu Leu Phe Ile Gly Leu Gly Ile 
                405                 410                 415     


Phe Phe Cys Val Arg Cys Arg His Arg Arg Arg Gln Ala Glu Arg Met 
            420                 425                 430         


Ser Gln Ile Lys Arg Leu Leu Ser Glu Lys Lys Thr Cys Gln Cys Pro 
        435                 440                 445             


His Arg Phe Gln Lys Thr Cys Ser Pro Ile 
    450                 455             


<210>  2
<211>  114
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Gly Cys Arg Leu Leu Cys Cys Ala Val Leu Cys Leu Leu Gly Ala 
1               5                   10                  15      


Val Pro Ile Asp Thr Glu Val Thr Gln Thr Pro Lys His Leu Val Met 
            20                  25                  30          


Gly Met Thr Asn Lys Lys Ser Leu Lys Cys Glu Gln His Met Gly His 
        35                  40                  45              


Arg Ala Met Tyr Trp Tyr Lys Gln Lys Ala Lys Lys Pro Pro Glu Leu 
    50                  55                  60                  


Met Phe Val Tyr Ser Tyr Glu Lys Leu Ser Ile Asn Glu Ser Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Pro Glu Cys Pro Asn Ser Ser Leu Leu Asn Leu His 
                85                  90                  95      


Leu His Ala Leu Gln Pro Glu Asp Ser Ala Leu Tyr Leu Cys Ala Ser 
            100                 105                 110         


Ser Gln 
        


<210>  3
<211>  501
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PGK promoter

<400>  3
gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg       60

gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc      120

gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca      180

ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac      240

gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt      300

ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg      360

gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct      420

ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt      480

cctcattctc cgggcctttc g                                                501


<210>  4
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Signalling peptide

<400>  4

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp 
            20      


<210>  5
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Signalling peptide

<400>  5
atggagacag acacactcct gctatgggtg ctgctgctct gggttccagg ttccacaggt       60

gac                                                                     63


<210>  6
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  First coding sequence (VL for CD4)

<400>  6

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Val Thr Met Asn Cys Lys Ser Ser Gln Ser Leu Leu Tyr Ser 
            20                  25                  30          


Thr Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Ser Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Val Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln 
                85                  90                  95      


Tyr Tyr Ser Tyr Arg Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


<210>  7
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  First coding sequence (VL for CD4)

<400>  7
gacattgtga tgactcagag ccccgacagc ctggccgtct cactgggcga aagggtgacc       60

atgaattgta aatcttctca gagcctgctg tacagtacaa accagaaaaa ttacctggcc      120

tggtatcagc agaaacccgg ccagagccct aagctgctga tctattgggc aagtacccga      180

gagtcaggag tgccagacag attctccggg tctggaagtg gcacagactt caccctgaca      240

attagctccg tgcaggccga ggacgtggct gtctactatt gccagcagta ctatagctac      300

cgaactttcg gcgggggaac caaactggaa atcaag                                336


<210>  8
<211>  122
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  First coding sequence (VH for CD4)

<400>  8

Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Val Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Val Ile His Trp Val Arg Gln Lys Pro Gly Gln Gly Leu Asp Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Tyr Asn Asp Gly Thr Asp Tyr Asp Glu Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ser Asp Thr Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Glu Lys Asp Asn Tyr Ala Thr Gly Ala Trp Phe Ala Tyr Trp 
            100                 105                 110         


Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120         


<210>  9
<211>  366
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  First coding sequence (VH for CD4)

<400>  9
caggtgcagc tgcagcagtc cggaccagag gtggtcaaac ccggcgctag cgtcaaaatg       60

tcctgtaagg catctggcta cactttcacc tcttatgtga ttcactgggt cagacagaag      120

cctgggcagg gactggactg gatcgggtac attaacccat ataatgatgg aactgactac      180

gatgaaaagt ttaaaggcaa ggccacactg acttccgaca cctcaacaag cactgcttat      240

atggagctgt ctagtctgag gtctgaagac acagcagtgt actattgcgc ccgcgagaag      300

gataactacg ccactggcgc ttggtttgca tattggggcc aggggaccct ggtgacagtc      360

tcatcc                                                                 366


<210>  10
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  G4S linker sequence

<400>  10

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  11
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G4S linker sequence

<400>  11
gaggaggagg cagtggcgga ggagggtcag gaggaggagg aagc                        44


<210>  12
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  First coding sequence (VL for TCR V-beta)

<400>  12

Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr 
            20                  25                  30          


Trp Ile Thr Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Asp Ile Tyr Pro Gly Ser Gly Phe Thr Lys Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Ser Lys Ala Thr Leu Thr Val Asp Thr Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Glu Gly Gly Asn Tyr Trp Tyr Phe Asp Val Trp Gly Thr Gly 
            100                 105                 110         


Thr Thr Val Thr Val Ser Ser 
        115                 


<210>  13
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  First coding sequence (VL for TCR V-beta)

<400>  13
caagttcagc tgcaacagcc tggcgccgag cttgtgaaac ctggcgcctc tgtgaagatg       60

agctgcaagg cctccggcta caccttcacc agatactgga tcacctgggt caagcagagg      120

cctggacagg gactcgagtg gatcggcgat atctatcctg gctccggctt caccaagtac      180

aacgagaagt tcaagagcaa ggccacactg accgtggaca ccagcagcag cacagcctac      240

atgcagctgt ctagcctgac cagcgaggac agcgccgtgt actactgtgc tagagaaggc      300

ggcaactact ggtacttcga cgtgtggggc accggcacca cagtgacagt tagttct         357


<210>  14
<211>  83
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8a hinge and transmembrane domain

<400>  14

Phe Val Pro Val Phe Leu Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro 
1               5                   10                  15      


Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu 
            20                  25                  30          


Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg 
        35                  40                  45              


Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly 
    50                  55                  60                  


Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn 
65                  70                  75                  80  


His Arg Asn 
            


<210>  15
<211>  249
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD8a hinge and transmembrane domain

<400>  15
ttcgtgccgg tcttcctgcc agcgaagccc accacgacgc cagcgccgcg accaccaaca       60

ccggcgccca ccatcgcgtc gcagcccctg tccctgcgcc cagaggcgtg ccggccagcg      120

gcggggggcg cagtgcacac gagggggctg gacttcgcct gtgatatcta catctgggcg      180

cccttggccg ggacttgtgg ggtccttctc ctgtcactgg ttatcaccct ttactgcaac      240

cacaggaac                                                              249


<210>  16
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 signalling domain

<400>  16

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  17
<211>  123
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD28 signalling domain

<400>  17
aggagtaaga ggagcaggct cctgcacagt gactacatga acatgactcc ccgccgcccc       60

gggcccaccc gcaagcatta ccagccctat gccccaccac gcgacttcgc agcctatcgc      120

tcc                                                                    123


<210>  18
<211>  47
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4-1BB signalling domain

<400>  18

Arg Phe Ser Val Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe 
1               5                   10                  15      


Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly 
            20                  25                  30          


Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40                  45          


<210>  19
<211>  141
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  4-1BB signalling domain

<400>  19
cgtttctctg ttgttaaacg gggcagaaag aagctcctgt atatattcaa acaaccattt       60

atgagaccag tacaaactac tcaagaggaa gatggctgta gctgccgatt tccagaagaa      120

gaagaaggag gatgtgaact g                                                141


<210>  20
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3zeta chain

<400>  20

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  21
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD3zeta chain

<400>  21
agagtgaagt tcagcaggag cgcagacgcc cccgcgtacc agcagggcca gaaccagctc       60

tataacgagc tcaatctagg acgaagagag gagtacgatg ttttggacaa gagacgtggc      120

cgggaccctg agatgggggg aaagccgaga aggaagaacc ctcaggaagg cctgtacaat      180

gaactgcaga aagataagat ggcggaggcc tacagtgaga ttgggatgaa aggcgagcgc      240

cggaggggca aggggcacga tggcctttac cagggtctca gtacagccac caaggacacc      300

tacgacgccc ttcacatgca ggccctgccc cctcgc                                336


<210>  22
<211>  357
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Truncated EGFR

<400>  22

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Arg Lys Val Cys Asn Gly Ile Gly Ile Gly 
            20                  25                  30          


Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala Thr Asn Ile Lys His Phe 
        35                  40                  45              


Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu His Ile Leu Pro Val Ala 
    50                  55                  60                  


Phe Arg Gly Asp Ser Phe Thr His Thr Pro Pro Leu Asp Pro Gln Glu 
65                  70                  75                  80  


Leu Asp Ile Leu Lys Thr Val Lys Glu Ile Thr Gly Phe Leu Leu Ile 
                85                  90                  95      


Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu His Ala Phe Glu Asn Leu 
            100                 105                 110         


Glu Ile Ile Arg Gly Arg Thr Lys Gln His Gly Gln Phe Ser Leu Ala 
        115                 120                 125             


Val Val Ser Leu Asn Ile Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu 
    130                 135                 140                 


Ile Ser Asp Gly Asp Val Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr 
145                 150                 155                 160 


Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys 
                165                 170                 175     


Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly 
            180                 185                 190         


Gln Val Cys His Ala Leu Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu 
        195                 200                 205             


Pro Arg Asp Cys Val Ser Cys Arg Asn Val Ser Arg Gly Arg Glu Cys 
    210                 215                 220                 


Val Asp Lys Cys Asn Leu Leu Glu Gly Glu Pro Arg Glu Phe Val Glu 
225                 230                 235                 240 


Asn Ser Glu Cys Ile Gln Cys His Pro Glu Cys Leu Pro Gln Ala Met 
                245                 250                 255     


Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala 
            260                 265                 270         


His Tyr Ile Asp Gly Pro His Cys Val Lys Thr Cys Pro Ala Gly Val 
        275                 280                 285             


Met Gly Glu Asn Asn Thr Leu Val Trp Lys Tyr Ala Asp Ala Gly His 
    290                 295                 300                 


Val Cys His Leu Cys His Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro 
305                 310                 315                 320 


Gly Leu Glu Gly Cys Pro Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala 
                325                 330                 335     


Thr Gly Met Val Gly Ala Leu Leu Leu Leu Leu Val Val Ala Leu Gly 
            340                 345                 350         


Ile Gly Leu Phe Met 
        355         


<210>  23
<211>  1071
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Truncated EGFR

<400>  23
atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg       60

atcccacgca aagtgtgtaa cggaataggt attggtgaat ttaaagactc actctccata      120

aatgctacga atattaaaca cttcaaaaac tgcacctcca tcagtggcga tctccacatc      180

ctgccggtgg catttagggg tgactccttc acacatactc ctcctctgga tccacaggaa      240

ctggatattc tgaaaaccgt aaaggaaatc acagggtttt tgctgattca ggcttggcct      300

gaaaacagga cggacctcca tgcctttgag aacctagaaa tcatacgcgg caggaccaag      360

caacatggtc agttttctct tgcagtcgtc agcctgaaca taacatcctt gggattacgc      420

tccctcaagg agataagtga tggagatgtg ataatttcag gaaacaaaaa tttgtgctat      480

gcaaatacaa taaactggaa aaaactgttt gggacctccg gtcagaaaac caaaattata      540

agcaacagag gtgaaaacag ctgcaaggcc acaggccagg tctgccatgc cttgtgctcc      600

cccgagggct gctggggccc ggaacccagg gactgcgtct cttgccggaa tgtcagccga      660

ggcagggaat gcgtggacaa gtgcaacctt ctggagggtg agccaaggga gtttgtggag      720

aactctgagt gcatacagtg ccacccagag tgcctgcctc aggccatgaa catcacctgc      780

acaggacggg gaccagacaa ctgtatccag tgtgcccact acattgacgg cccccactgc      840

gtcaagacct gcccggcagg agtcatggga gaaaacaaca ccctggtctg gaagtacgca      900

gacgccggcc atgtgtgcca cctgtgccat ccaaactgca cctacggatg cactgggcca      960

ggtcttgaag gctgtccaac gaatgggcct aagatcccgt ccatcgccac tgggatggtg     1020

ggggccctcc tcttgctgct ggtggtggcc ctggggatcg gcctcttcat g              1071


<210>  24
<211>  413
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Inducible caspase-9 (iC9)

<400>  24

Met Leu Glu Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg 
1               5                   10                  15      


Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met 
            20                  25                  30          


Leu Glu Asp Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys Pro 
        35                  40                  45              


Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu 
    50                  55                  60                  


Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser 
65                  70                  75                  80  


Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro 
                85                  90                  95      


His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Ser Gly 
            100                 105                 110         


Gly Gly Ser Gly Val Asp Gly Phe Gly Asp Val Gly Ala Leu Glu Ser 
        115                 120                 125             


Leu Arg Gly Asn Ala Asp Leu Ala Tyr Ile Leu Ser Met Glu Pro Cys 
    130                 135                 140                 


Gly His Cys Leu Ile Ile Asn Asn Val Asn Phe Cys Arg Glu Ser Gly 
145                 150                 155                 160 


Leu Arg Thr Arg Thr Gly Ser Asn Ile Asp Cys Glu Lys Leu Arg Arg 
                165                 170                 175     


Arg Phe Ser Ser Leu His Phe Met Val Glu Val Lys Gly Asp Leu Thr 
            180                 185                 190         


Ala Lys Lys Met Val Leu Ala Leu Leu Glu Leu Ala Gln Gln Asp His 
        195                 200                 205             


Gly Ala Leu Asp Cys Cys Val Val Val Ile Leu Ser His Gly Cys Gln 
    210                 215                 220                 


Ala Ser His Leu Gln Phe Pro Gly Ala Val Tyr Gly Thr Asp Gly Cys 
225                 230                 235                 240 


Pro Val Ser Val Glu Lys Ile Val Asn Ile Phe Asn Gly Thr Ser Cys 
                245                 250                 255     


Pro Ser Leu Gly Gly Lys Pro Lys Leu Phe Phe Ile Gln Ala Cys Gly 
            260                 265                 270         


Gly Glu Gln Lys Asp His Gly Phe Glu Val Ala Ser Thr Ser Pro Glu 
        275                 280                 285             


Asp Glu Ser Pro Gly Ser Asn Pro Glu Pro Asp Ala Thr Pro Phe Gln 
    290                 295                 300                 


Glu Gly Leu Arg Thr Phe Asp Gln Leu Asp Ala Ile Ser Ser Leu Pro 
305                 310                 315                 320 


Thr Pro Ser Asp Ile Phe Val Ser Tyr Ser Thr Phe Pro Gly Phe Val 
                325                 330                 335     


Ser Trp Arg Asp Pro Lys Ser Gly Ser Trp Tyr Val Glu Thr Leu Asp 
            340                 345                 350         


Asp Ile Phe Glu Gln Trp Ala His Ser Glu Asp Leu Gln Ser Leu Leu 
        355                 360                 365             


Leu Arg Val Ala Asn Ala Val Ser Val Lys Gly Ile Tyr Lys Gln Met 
    370                 375                 380                 


Pro Gly Cys Phe Asn Phe Leu Arg Lys Lys Leu Phe Phe Lys Thr Ser 
385                 390                 395                 400 


Val Asp Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Leu Asp 
                405                 410             


<210>  25
<211>  1242
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Inducible caspase-9 (iC9)

<400>  25
atgctcgagg gagtgcaggt ggaaaccatc tccccaggag acgggcgcac cttccccaag       60

cgcggccaga cctgcgtggt gcactacacc gggatgcttg aagatggaaa gaaagttgat      120

tcctcccggg acagaaacaa gccctttaag tttatgctag gcaagcagga ggtgatccga      180

ggctgggaag aaggggttgc ccagatgagt gtgggtcaga gagccaaact gactatatct      240

ccagattatg cctatggtgc cactgggcac ccaggcatca tcccaccaca tgccactctc      300

gtcttcgatg tggagcttct aaaactggaa tctggcggtg gatccggagt cgacggattt      360

ggtgatgtcg gtgctcttga gagtttgagg ggaaatgcag atttggctta catcctgagc      420

atggagccct gtggccactg cctcattatc aacaatgtga acttctgccg tgagtccggg      480

ctccgcaccc gcactggctc caacatcgac tgtgagaagt tgcggcgtcg cttctcctcg      540

ctgcatttca tggtggaggt gaagggcgac ctgactgcca agaaaatggt gctggctttg      600

ctggagctgg cgcagcagga ccacggtgct ctggactgct gcgtggtggt cattctctct      660

cacggctgtc aggccagcca cctgcagttc ccaggggctg tctacggcac agatggatgc      720

cctgtgtcgg tcgagaagat tgtgaacatc ttcaatggga ccagctgccc cagcctggga      780

gggaagccca agctcttttt catccaggcc tgtggtgggg agcagaaaga ccatgggttt      840

gaggtggcct ccacttcccc tgaagacgag tcccctggca gtaaccccga gccagatgcc      900

accccgttcc aggaaggttt gaggaccttc gaccagctgg acgccatatc tagtttgccc      960

acacccagtg acatctttgt gtcctactct actttcccag gttttgtttc ctggagggac     1020

cccaagagtg gctcctggta cgttgagacc ctggacgaca tctttgagca gtgggctcac     1080

tctgaagacc tgcagtccct cctgcttagg gtcgctaatg ctgtttcggt gaaagggatt     1140

tataaacaga tgcctggttg ctttaatttc ctccggaaaa aacttttctt taaaacatca     1200

gtcgactatc cgtacgacgt accagactac gcactcgact aa                        1242


<210>  26
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P2A spacer

<400>  26

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  27
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P2A spacer

<400>  27
ggatccggag ccacgaactt ctctctgtta aagcaagcag gagacgtgga agaaaacccc       60

ggtcct                                                                  66


<210>  28
<211>  592
<212>  DNA
<213>  Woodchuck hepatitis virus

<400>  28
aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct       60

ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt      120

atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg      180

tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact      240

ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct      300

attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg      360

ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc      420

gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc      480

aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt      540

cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tg              592


<210>  29
<211>  1370
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nucleic acid construct (CART4)

<400>  29

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu 
            20                  25                  30          


Ala Val Ser Leu Gly Glu Arg Val Thr Met Asn Cys Lys Ser Ser Gln 
        35                  40                  45              


Ser Leu Leu Tyr Ser Thr Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln 
    50                  55                  60                  


Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr 
65                  70                  75                  80  


Arg Glu Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr 
                85                  90                  95      


Asp Phe Thr Leu Thr Ile Ser Ser Val Gln Ala Glu Asp Val Ala Val 
            100                 105                 110         


Tyr Tyr Cys Gln Gln Tyr Tyr Ser Tyr Arg Thr Phe Gly Gly Gly Thr 
        115                 120                 125             


Lys Leu Glu Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Val Val 
145                 150                 155                 160 


Lys Pro Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr 
                165                 170                 175     


Phe Thr Ser Tyr Val Ile His Trp Val Arg Gln Lys Pro Gly Gln Gly 
            180                 185                 190         


Leu Asp Trp Ile Gly Tyr Ile Asn Pro Tyr Asn Asp Gly Thr Asp Tyr 
        195                 200                 205             


Asp Glu Lys Phe Lys Gly Lys Ala Thr Leu Thr Ser Asp Thr Ser Thr 
    210                 215                 220                 


Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala 
225                 230                 235                 240 


Val Tyr Tyr Cys Ala Arg Glu Lys Asp Asn Tyr Ala Thr Gly Ala Trp 
                245                 250                 255     


Phe Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ala 
            260                 265                 270         


Ala Phe Val Pro Val Phe Leu Pro Ala Lys Pro Thr Thr Thr Pro Ala 
        275                 280                 285             


Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser 
    290                 295                 300                 


Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr 
305                 310                 315                 320 


Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala 
                325                 330                 335     


Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys 
            340                 345                 350         


Asn His Arg Asn Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr 
        355                 360                 365             


Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln 
    370                 375                 380                 


Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Phe Ser 
385                 390                 395                 400 


Val Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro 
                405                 410                 415     


Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys 
            420                 425                 430         


Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe 
        435                 440                 445             


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
    450                 455                 460                 


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
465                 470                 475                 480 


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
                485                 490                 495     


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
            500                 505                 510         


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
        515                 520                 525             


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
    530                 535                 540                 


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Gly Ser Gly Ala 
545                 550                 555                 560 


Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro 
                565                 570                 575     


Gly Pro Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro 
            580                 585                 590         


His Pro Ala Phe Leu Leu Ile Pro Arg Lys Val Cys Asn Gly Ile Gly 
        595                 600                 605             


Ile Gly Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala Thr Asn Ile Lys 
    610                 615                 620                 


His Phe Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu His Ile Leu Pro 
625                 630                 635                 640 


Val Ala Phe Arg Gly Asp Ser Phe Thr His Thr Pro Pro Leu Asp Pro 
                645                 650                 655     


Gln Glu Leu Asp Ile Leu Lys Thr Val Lys Glu Ile Thr Gly Phe Leu 
            660                 665                 670         


Leu Ile Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu His Ala Phe Glu 
        675                 680                 685             


Asn Leu Glu Ile Ile Arg Gly Arg Thr Lys Gln His Gly Gln Phe Ser 
    690                 695                 700                 


Leu Ala Val Val Ser Leu Asn Ile Thr Ser Leu Gly Leu Arg Ser Leu 
705                 710                 715                 720 


Lys Glu Ile Ser Asp Gly Asp Val Ile Ile Ser Gly Asn Lys Asn Leu 
                725                 730                 735     


Cys Tyr Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe Gly Thr Ser Gly 
            740                 745                 750         


Gln Lys Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn Ser Cys Lys Ala 
        755                 760                 765             


Thr Gly Gln Val Cys His Ala Leu Cys Ser Pro Glu Gly Cys Trp Gly 
    770                 775                 780                 


Pro Glu Pro Arg Asp Cys Val Ser Cys Arg Asn Val Ser Arg Gly Arg 
785                 790                 795                 800 


Glu Cys Val Asp Lys Cys Asn Leu Leu Glu Gly Glu Pro Arg Glu Phe 
                805                 810                 815     


Val Glu Asn Ser Glu Cys Ile Gln Cys His Pro Glu Cys Leu Pro Gln 
            820                 825                 830         


Ala Met Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp Asn Cys Ile Gln 
        835                 840                 845             


Cys Ala His Tyr Ile Asp Gly Pro His Cys Val Lys Thr Cys Pro Ala 
    850                 855                 860                 


Gly Val Met Gly Glu Asn Asn Thr Leu Val Trp Lys Tyr Ala Asp Ala 
865                 870                 875                 880 


Gly His Val Cys His Leu Cys His Pro Asn Cys Thr Tyr Gly Cys Thr 
                885                 890                 895     


Gly Pro Gly Leu Glu Gly Cys Pro Thr Asn Gly Pro Lys Ile Pro Ser 
            900                 905                 910         


Ile Ala Thr Gly Met Val Gly Ala Leu Leu Leu Leu Leu Val Val Ala 
        915                 920                 925             


Leu Gly Ile Gly Leu Phe Met Gly Ser Gly Ala Thr Asn Phe Ser Leu 
    930                 935                 940                 


Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Leu Glu 
945                 950                 955                 960 


Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro 
                965                 970                 975     


Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp 
            980                 985                 990         


Gly Lys Lys Val Asp Ser Ser Arg  Asp Arg Asn Lys Pro  Phe Lys Phe 
        995                 1000                 1005             


Met Leu  Gly Lys Gln Glu Val  Ile Arg Gly Trp Glu  Glu Gly Val 
    1010                 1015                 1020             


Ala Gln  Met Ser Val Gly Gln  Arg Ala Lys Leu Thr  Ile Ser Pro 
    1025                 1030                 1035             


Asp Tyr  Ala Tyr Gly Ala Thr  Gly His Pro Gly Ile  Ile Pro Pro 
    1040                 1045                 1050             


His Ala  Thr Leu Val Phe Asp  Val Glu Leu Leu Lys  Leu Glu Ser 
    1055                 1060                 1065             


Gly Gly  Gly Ser Gly Val Asp  Gly Phe Gly Asp Val  Gly Ala Leu 
    1070                 1075                 1080             


Glu Ser  Leu Arg Gly Asn Ala  Asp Leu Ala Tyr Ile  Leu Ser Met 
    1085                 1090                 1095             


Glu Pro  Cys Gly His Cys Leu  Ile Ile Asn Asn Val  Asn Phe Cys 
    1100                 1105                 1110             


Arg Glu  Ser Gly Leu Arg Thr  Arg Thr Gly Ser Asn  Ile Asp Cys 
    1115                 1120                 1125             


Glu Lys  Leu Arg Arg Arg Phe  Ser Ser Leu His Phe  Met Val Glu 
    1130                 1135                 1140             


Val Lys  Gly Asp Leu Thr Ala  Lys Lys Met Val Leu  Ala Leu Leu 
    1145                 1150                 1155             


Glu Leu  Ala Gln Gln Asp His  Gly Ala Leu Asp Cys  Cys Val Val 
    1160                 1165                 1170             


Val Ile  Leu Ser His Gly Cys  Gln Ala Ser His Leu  Gln Phe Pro 
    1175                 1180                 1185             


Gly Ala  Val Tyr Gly Thr Asp  Gly Cys Pro Val Ser  Val Glu Lys 
    1190                 1195                 1200             


Ile Val  Asn Ile Phe Asn Gly  Thr Ser Cys Pro Ser  Leu Gly Gly 
    1205                 1210                 1215             


Lys Pro  Lys Leu Phe Phe Ile  Gln Ala Cys Gly Gly  Glu Gln Lys 
    1220                 1225                 1230             


Asp His  Gly Phe Glu Val Ala  Ser Thr Ser Pro Glu  Asp Glu Ser 
    1235                 1240                 1245             


Pro Gly  Ser Asn Pro Glu Pro  Asp Ala Thr Pro Phe  Gln Glu Gly 
    1250                 1255                 1260             


Leu Arg  Thr Phe Asp Gln Leu  Asp Ala Ile Ser Ser  Leu Pro Thr 
    1265                 1270                 1275             


Pro Ser  Asp Ile Phe Val Ser  Tyr Ser Thr Phe Pro  Gly Phe Val 
    1280                 1285                 1290             


Ser Trp  Arg Asp Pro Lys Ser  Gly Ser Trp Tyr Val  Glu Thr Leu 
    1295                 1300                 1305             


Asp Asp  Ile Phe Glu Gln Trp  Ala His Ser Glu Asp  Leu Gln Ser 
    1310                 1315                 1320             


Leu Leu  Leu Arg Val Ala Asn  Ala Val Ser Val Lys  Gly Ile Tyr 
    1325                 1330                 1335             


Lys Gln  Met Pro Gly Cys Phe  Asn Phe Leu Arg Lys  Lys Leu Phe 
    1340                 1345                 1350             


Phe Lys  Thr Ser Val Asp Tyr  Pro Tyr Asp Val Pro  Asp Tyr Ala 
    1355                 1360                 1365             


Leu Asp  
    1370 


<210>  30
<211>  4113
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid construct (CART4)

<400>  30
atggagacag acacactcct gctatgggtg ctgctgctct gggttccagg ttccacaggt       60

gacgacattg tgatgactca gagccccgac agcctggccg tctcactggg cgaaagggtg      120

accatgaatt gtaaatcttc tcagagcctg ctgtacagta caaaccagaa aaattacctg      180

gcctggtatc agcagaaacc cggccagagc cctaagctgc tgatctattg ggcaagtacc      240

cgagagtcag gagtgccaga cagattctcc gggtctggaa gtggcacaga cttcaccctg      300

acaattagct ccgtgcaggc cgaggacgtg gctgtctact attgccagca gtactatagc      360

taccgaactt tcggcggggg aaccaaactg gaaatcaagg gaggaggagg cagtggcgga      420

ggagggtcag gaggaggagg aagccaggtg cagctgcagc agtccggacc agaggtggtc      480

aaacccggcg ctagcgtcaa aatgtcctgt aaggcatctg gctacacttt cacctcttat      540

gtgattcact gggtcagaca gaagcctggg cagggactgg actggatcgg gtacattaac      600

ccatataatg atggaactga ctacgatgaa aagtttaaag gcaaggccac actgacttcc      660

gacacctcaa caagcactgc ttatatggag ctgtctagtc tgaggtctga agacacagca      720

gtgtactatt gcgcccgcga gaaggataac tacgccactg gcgcttggtt tgcatattgg      780

ggccagggga ccctggtgac agtctcatcc gcggccgcat tcgtgccggt cttcctgcca      840

gcgaagccca ccacgacgcc agcgccgcga ccaccaacac cggcgcccac catcgcgtcg      900

cagcccctgt ccctgcgccc agaggcgtgc cggccagcgg cggggggcgc agtgcacacg      960

agggggctgg acttcgcctg tgatatctac atctgggcgc ccttggccgg gacttgtggg     1020

gtccttctcc tgtcactggt tatcaccctt tactgcaacc acaggaacag gagtaagagg     1080

agcaggctcc tgcacagtga ctacatgaac atgactcccc gccgccccgg gcccacccgc     1140

aagcattacc agccctatgc cccaccacgc gacttcgcag cctatcgctc ccgtttctct     1200

gttgttaaac ggggcagaaa gaagctcctg tatatattca aacaaccatt tatgagacca     1260

gtacaaacta ctcaagagga agatggctgt agctgccgat ttccagaaga agaagaagga     1320

ggatgtgaac tgagagtgaa gttcagcagg agcgcagacg cccccgcgta ccagcagggc     1380

cagaaccagc tctataacga gctcaatcta ggacgaagag aggagtacga tgttttggac     1440

aagagacgtg gccgggaccc tgagatgggg ggaaagccga gaaggaagaa ccctcaggaa     1500

ggcctgtaca atgaactgca gaaagataag atggcggagg cctacagtga gattgggatg     1560

aaaggcgagc gccggagggg caaggggcac gatggccttt accagggtct cagtacagcc     1620

accaaggaca cctacgacgc ccttcacatg caggccctgc cccctcgcgg atccggagcc     1680

acgaacttct ctctgttaaa gcaagcagga gacgtggaag aaaaccccgg tcctatgctt     1740

ctcctggtga caagccttct gctctgtgag ttaccacacc cagcattcct cctgatccca     1800

cgcaaagtgt gtaacggaat aggtattggt gaatttaaag actcactctc cataaatgct     1860

acgaatatta aacacttcaa aaactgcacc tccatcagtg gcgatctcca catcctgccg     1920

gtggcattta ggggtgactc cttcacacat actcctcctc tggatccaca ggaactggat     1980

attctgaaaa ccgtaaagga aatcacaggg tttttgctga ttcaggcttg gcctgaaaac     2040

aggacggacc tccatgcctt tgagaaccta gaaatcatac gcggcaggac caagcaacat     2100

ggtcagtttt ctcttgcagt cgtcagcctg aacataacat ccttgggatt acgctccctc     2160

aaggagataa gtgatggaga tgtgataatt tcaggaaaca aaaatttgtg ctatgcaaat     2220

acaataaact ggaaaaaact gtttgggacc tccggtcaga aaaccaaaat tataagcaac     2280

agaggtgaaa acagctgcaa ggccacaggc caggtctgcc atgccttgtg ctcccccgag     2340

ggctgctggg gcccggaacc cagggactgc gtctcttgcc ggaatgtcag ccgaggcagg     2400

gaatgcgtgg acaagtgcaa ccttctggag ggtgagccaa gggagtttgt ggagaactct     2460

gagtgcatac agtgccaccc agagtgcctg cctcaggcca tgaacatcac ctgcacagga     2520

cggggaccag acaactgtat ccagtgtgcc cactacattg acggccccca ctgcgtcaag     2580

acctgcccgg caggagtcat gggagaaaac aacaccctgg tctggaagta cgcagacgcc     2640

ggccatgtgt gccacctgtg ccatccaaac tgcacctacg gatgcactgg gccaggtctt     2700

gaaggctgtc caacgaatgg gcctaagatc ccgtccatcg ccactgggat ggtgggggcc     2760

ctcctcttgc tgctggtggt ggccctgggg atcggcctct tcatgggatc tggagccacg     2820

aacttctctc tgttaaagca agcaggagac gtggaagaaa accccggtcc tatgctcgag     2880

ggagtgcagg tggaaaccat ctccccagga gacgggcgca ccttccccaa gcgcggccag     2940

acctgcgtgg tgcactacac cgggatgctt gaagatggaa agaaagttga ttcctcccgg     3000

gacagaaaca agccctttaa gtttatgcta ggcaagcagg aggtgatccg aggctgggaa     3060

gaaggggttg cccagatgag tgtgggtcag agagccaaac tgactatatc tccagattat     3120

gcctatggtg ccactgggca cccaggcatc atcccaccac atgccactct cgtcttcgat     3180

gtggagcttc taaaactgga atctggcggt ggatccggag tcgacggatt tggtgatgtc     3240

ggtgctcttg agagtttgag gggaaatgca gatttggctt acatcctgag catggagccc     3300

tgtggccact gcctcattat caacaatgtg aacttctgcc gtgagtccgg gctccgcacc     3360

cgcactggct ccaacatcga ctgtgagaag ttgcggcgtc gcttctcctc gctgcatttc     3420

atggtggagg tgaagggcga cctgactgcc aagaaaatgg tgctggcttt gctggagctg     3480

gcgcagcagg accacggtgc tctggactgc tgcgtggtgg tcattctctc tcacggctgt     3540

caggccagcc acctgcagtt cccaggggct gtctacggca cagatggatg ccctgtgtcg     3600

gtcgagaaga ttgtgaacat cttcaatggg accagctgcc ccagcctggg agggaagccc     3660

aagctctttt tcatccaggc ctgtggtggg gagcagaaag accatgggtt tgaggtggcc     3720

tccacttccc ctgaagacga gtcccctggc agtaaccccg agccagatgc caccccgttc     3780

caggaaggtt tgaggacctt cgaccagctg gacgccatat ctagtttgcc cacacccagt     3840

gacatctttg tgtcctactc tactttccca ggttttgttt cctggaggga ccccaagagt     3900

ggctcctggt acgttgagac cctggacgac atctttgagc agtgggctca ctctgaagac     3960

ctgcagtccc tcctgcttag ggtcgctaat gctgtttcgg tgaaagggat ttataaacag     4020

atgcctggtt gctttaattt cctccggaaa aaacttttct ttaaaacatc agtcgactat     4080

ccgtacgacg taccagacta cgcactcgac taa                                  4113


<210>  31
<211>  1366
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nucleic acid construct (CARTVb7.1)

<400>  31

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Gly Lys Val Thr Leu Thr Cys Lys Ala Ser Gln 
        35                  40                  45              


Asp Ile Asn Lys Tyr Ile Ala Trp Tyr Gln His Lys Pro Gly Lys Gly 
    50                  55                  60                  


Pro Arg Leu Leu Ile His Tyr Thr Ser Thr Leu Gln Pro Gly Ile Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Arg Asp Tyr Ser Phe Ser Ile 
                85                  90                  95      


Ser Asn Leu Glu Pro Glu Asp Val Ala Thr Tyr Tyr Cys Leu Gln Tyr 
            100                 105                 110         


Asp Asn Leu Arg Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
        115                 120                 125             


Thr Asp Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys Pro Gly 
145                 150                 155                 160 


Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg 
                165                 170                 175     


Tyr Trp Ile Thr Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp 
            180                 185                 190         


Ile Gly Asp Ile Tyr Pro Gly Ser Gly Phe Thr Lys Tyr Asn Glu Lys 
        195                 200                 205             


Phe Lys Ser Lys Ala Thr Leu Thr Val Asp Thr Ser Ser Ser Thr Ala 
    210                 215                 220                 


Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr 
225                 230                 235                 240 


Cys Ala Arg Glu Gly Gly Asn Tyr Trp Tyr Phe Asp Val Trp Gly Thr 
                245                 250                 255     


Gly Thr Thr Val Thr Val Ser Ser Ala Ala Ala Ala Ala Phe Val Pro 
            260                 265                 270         


Val Phe Leu Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro 
        275                 280                 285             


Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu 
    290                 295                 300                 


Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp 
305                 310                 315                 320 


Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
                325                 330                 335     


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg Asn 
            340                 345                 350         


Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
        355                 360                 365             


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
    370                 375                 380                 


Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Phe Ser Val Val Lys Arg 
385                 390                 395                 400 


Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro 
                405                 410                 415     


Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu 
            420                 425                 430         


Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala 
        435                 440                 445             


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
    450                 455                 460                 


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
465                 470                 475                 480 


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
                485                 490                 495     


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
            500                 505                 510         


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
        515                 520                 525             


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
    530                 535                 540                 


His Met Gln Ala Leu Pro Pro Arg Gly Ser Gly Ala Thr Asn Phe Ser 
545                 550                 555                 560 


Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Leu 
                565                 570                 575     


Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro Ala Phe 
            580                 585                 590         


Leu Leu Ile Pro Arg Lys Val Cys Asn Gly Ile Gly Ile Gly Glu Phe 
        595                 600                 605             


Lys Asp Ser Leu Ser Ile Asn Ala Thr Asn Ile Lys His Phe Lys Asn 
    610                 615                 620                 


Cys Thr Ser Ile Ser Gly Asp Leu His Ile Leu Pro Val Ala Phe Arg 
625                 630                 635                 640 


Gly Asp Ser Phe Thr His Thr Pro Pro Leu Asp Pro Gln Glu Leu Asp 
                645                 650                 655     


Ile Leu Lys Thr Val Lys Glu Ile Thr Gly Phe Leu Leu Ile Gln Ala 
            660                 665                 670         


Trp Pro Glu Asn Arg Thr Asp Leu His Ala Phe Glu Asn Leu Glu Ile 
        675                 680                 685             


Ile Arg Gly Arg Thr Lys Gln His Gly Gln Phe Ser Leu Ala Val Val 
    690                 695                 700                 


Ser Leu Asn Ile Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu Ile Ser 
705                 710                 715                 720 


Asp Gly Asp Val Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr Ala Asn 
                725                 730                 735     


Thr Ile Asn Trp Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys Thr Lys 
            740                 745                 750         


Ile Ile Ser Asn Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly Gln Val 
        755                 760                 765             


Cys His Ala Leu Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu Pro Arg 
    770                 775                 780                 


Asp Cys Val Ser Cys Arg Asn Val Ser Arg Gly Arg Glu Cys Val Asp 
785                 790                 795                 800 


Lys Cys Asn Leu Leu Glu Gly Glu Pro Arg Glu Phe Val Glu Asn Ser 
                805                 810                 815     


Glu Cys Ile Gln Cys His Pro Glu Cys Leu Pro Gln Ala Met Asn Ile 
            820                 825                 830         


Thr Cys Thr Gly Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala His Tyr 
        835                 840                 845             


Ile Asp Gly Pro His Cys Val Lys Thr Cys Pro Ala Gly Val Met Gly 
    850                 855                 860                 


Glu Asn Asn Thr Leu Val Trp Lys Tyr Ala Asp Ala Gly His Val Cys 
865                 870                 875                 880 


His Leu Cys His Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro Gly Leu 
                885                 890                 895     


Glu Gly Cys Pro Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala Thr Gly 
            900                 905                 910         


Met Val Gly Ala Leu Leu Leu Leu Leu Val Val Ala Leu Gly Ile Gly 
        915                 920                 925             


Leu Phe Met Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala 
    930                 935                 940                 


Gly Asp Val Glu Glu Asn Pro Gly Pro Met Leu Glu Gly Val Gln Val 
945                 950                 955                 960 


Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro Lys Arg Gly Gln 
                965                 970                 975     


Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp Gly Lys Lys Val 
            980                 985                 990         


Asp Ser Ser Arg Asp Arg Asn Lys  Pro Phe Lys Phe Met  Leu Gly Lys 
        995                 1000                 1005             


Gln Glu  Val Ile Arg Gly Trp  Glu Glu Gly Val Ala  Gln Met Ser 
    1010                 1015                 1020             


Val Gly  Gln Arg Ala Lys Leu  Thr Ile Ser Pro Asp  Tyr Ala Tyr 
    1025                 1030                 1035             


Gly Ala  Thr Gly His Pro Gly  Ile Ile Pro Pro His  Ala Thr Leu 
    1040                 1045                 1050             


Val Phe  Asp Val Glu Leu Leu  Lys Leu Glu Ser Gly  Gly Gly Ser 
    1055                 1060                 1065             


Gly Val  Asp Gly Phe Gly Asp  Val Gly Ala Leu Glu  Ser Leu Arg 
    1070                 1075                 1080             


Gly Asn  Ala Asp Leu Ala Tyr  Ile Leu Ser Met Glu  Pro Cys Gly 
    1085                 1090                 1095             


His Cys  Leu Ile Ile Asn Asn  Val Asn Phe Cys Arg  Glu Ser Gly 
    1100                 1105                 1110             


Leu Arg  Thr Arg Thr Gly Ser  Asn Ile Asp Cys Glu  Lys Leu Arg 
    1115                 1120                 1125             


Arg Arg  Phe Ser Ser Leu His  Phe Met Val Glu Val  Lys Gly Asp 
    1130                 1135                 1140             


Leu Thr  Ala Lys Lys Met Val  Leu Ala Leu Leu Glu  Leu Ala Gln 
    1145                 1150                 1155             


Gln Asp  His Gly Ala Leu Asp  Cys Cys Val Val Val  Ile Leu Ser 
    1160                 1165                 1170             


His Gly  Cys Gln Ala Ser His  Leu Gln Phe Pro Gly  Ala Val Tyr 
    1175                 1180                 1185             


Gly Thr  Asp Gly Cys Pro Val  Ser Val Glu Lys Ile  Val Asn Ile 
    1190                 1195                 1200             


Phe Asn  Gly Thr Ser Cys Pro  Ser Leu Gly Gly Lys  Pro Lys Leu 
    1205                 1210                 1215             


Phe Phe  Ile Gln Ala Cys Gly  Gly Glu Gln Lys Asp  His Gly Phe 
    1220                 1225                 1230             


Glu Val  Ala Ser Thr Ser Pro  Glu Asp Glu Ser Pro  Gly Ser Asn 
    1235                 1240                 1245             


Pro Glu  Pro Asp Ala Thr Pro  Phe Gln Glu Gly Leu  Arg Thr Phe 
    1250                 1255                 1260             


Asp Gln  Leu Asp Ala Ile Ser  Ser Leu Pro Thr Pro  Ser Asp Ile 
    1265                 1270                 1275             


Phe Val  Ser Tyr Ser Thr Phe  Pro Gly Phe Val Ser  Trp Arg Asp 
    1280                 1285                 1290             


Pro Lys  Ser Gly Ser Trp Tyr  Val Glu Thr Leu Asp  Asp Ile Phe 
    1295                 1300                 1305             


Glu Gln  Trp Ala His Ser Glu  Asp Leu Gln Ser Leu  Leu Leu Arg 
    1310                 1315                 1320             


Val Ala  Asn Ala Val Ser Val  Lys Gly Ile Tyr Lys  Gln Met Pro 
    1325                 1330                 1335             


Gly Cys  Phe Asn Phe Leu Arg  Lys Lys Leu Phe Phe  Lys Thr Ser 
    1340                 1345                 1350             


Val Asp  Tyr Pro Tyr Asp Val  Pro Asp Tyr Ala Leu  Asp 
    1355                 1360                 1365     


<210>  32
<211>  4101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid construct (CARTVb7.1)

<400>  32
atggctctgc ctgttacagc tctgctgctg cctctggctc tgcttctgca tgccgccaga       60

cctgacatcc agatgacaca gagccctagc agcctgtctg cctctctcgg cggaaaagtg      120

accctgacat gcaaggccag ccaggacatc aacaagtata tcgcctggta tcagcacaag      180

cccggcaagg gacctagact gctgatccac tacaccagca cactgcagcc tggcatcccc      240

agcagatttt ctggcagcgg ctccggcaga gactacagct tcagcatcag caacctggaa      300

cctgaggacg tggccaccta ctactgcctg cagtacgaca acctgcggac ctttggcggc      360

ggaacaaagc tggaaatcaa gcggacagat ggcggaggcg gatcaggcgg cggaggaagc      420

ggtggcggag gatctcaagt tcagctgcaa cagcctggcg ccgagcttgt gaaacctggc      480

gcctctgtga agatgagctg caaggcctcc ggctacacct tcaccagata ctggatcacc      540

tgggtcaagc agaggcctgg acagggactc gagtggatcg gcgatatcta tcctggctcc      600

ggcttcacca agtacaacga gaagttcaag agcaaggcca cactgaccgt ggacaccagc      660

agcagcacag cctacatgca gctgtctagc ctgaccagcg aggacagcgc cgtgtactac      720

tgtgctagag aaggcggcaa ctactggtac ttcgacgtgt ggggcaccgg caccacagtg      780

acagttagtt ctgcggccgc ggccgcattc gtgccggtct tcctgccagc gaagcccacc      840

acgacgccag cgccgcgacc accaacaccg gcgcccacca tcgcgtcgca gcccctgtcc      900

ctgcgcccag aggcgtgccg gccagcggcg gggggcgcag tgcacacgag ggggctggac      960

ttcgcctgtg atatctacat ctgggcgccc ttggccggga cttgtggggt ccttctcctg     1020

tcactggtta tcacccttta ctgcaaccac aggaacagga gtaagaggag caggctcctg     1080

cacagtgact acatgaacat gactccccgc cgccccgggc ccacccgcaa gcattaccag     1140

ccctatgccc caccacgcga cttcgcagcc tatcgctccc gtttctctgt tgttaaacgg     1200

ggcagaaaga agctcctgta tatattcaaa caaccattta tgagaccagt acaaactact     1260

caagaggaag atggctgtag ctgccgattt ccagaagaag aagaaggagg atgtgaactg     1320

agagtgaagt tcagcaggag cgcagacgcc cccgcgtacc agcagggcca gaaccagctc     1380

tataacgagc tcaatctagg acgaagagag gagtacgatg ttttggacaa gagacgtggc     1440

cgggaccctg agatgggggg aaagccgaga aggaagaacc ctcaggaagg cctgtacaat     1500

gaactgcaga aagataagat ggcggaggcc tacagtgaga ttgggatgaa aggcgagcgc     1560

cggaggggca aggggcacga tggcctttac cagggtctca gtacagccac caaggacacc     1620

tacgacgccc ttcacatgca ggccctgccc cctcgcggat ccggagccac gaacttctct     1680

ctgttaaagc aagcaggaga cgtggaagaa aaccccggtc ctatgcttct cctggtgaca     1740

agccttctgc tctgtgagtt accacaccca gcattcctcc tgatcccacg caaagtgtgt     1800

aacggaatag gtattggtga atttaaagac tcactctcca taaatgctac gaatattaaa     1860

cacttcaaaa actgcacctc catcagtggc gatctccaca tcctgccggt ggcatttagg     1920

ggtgactcct tcacacatac tcctcctctg gatccacagg aactggatat tctgaaaacc     1980

gtaaaggaaa tcacagggtt tttgctgatt caggcttggc ctgaaaacag gacggacctc     2040

catgcctttg agaacctaga aatcatacgc ggcaggacca agcaacatgg tcagttttct     2100

cttgcagtcg tcagcctgaa cataacatcc ttgggattac gctccctcaa ggagataagt     2160

gatggagatg tgataatttc aggaaacaaa aatttgtgct atgcaaatac aataaactgg     2220

aaaaaactgt ttgggacctc cggtcagaaa accaaaatta taagcaacag aggtgaaaac     2280

agctgcaagg ccacaggcca ggtctgccat gccttgtgct cccccgaggg ctgctggggc     2340

ccggaaccca gggactgcgt ctcttgccgg aatgtcagcc gaggcaggga atgcgtggac     2400

aagtgcaacc ttctggaggg tgagccaagg gagtttgtgg agaactctga gtgcatacag     2460

tgccacccag agtgcctgcc tcaggccatg aacatcacct gcacaggacg gggaccagac     2520

aactgtatcc agtgtgccca ctacattgac ggcccccact gcgtcaagac ctgcccggca     2580

ggagtcatgg gagaaaacaa caccctggtc tggaagtacg cagacgccgg ccatgtgtgc     2640

cacctgtgcc atccaaactg cacctacgga tgcactgggc caggtcttga aggctgtcca     2700

acgaatgggc ctaagatccc gtccatcgcc actgggatgg tgggggccct cctcttgctg     2760

ctggtggtgg ccctggggat cggcctcttc atgggatctg gagccacgaa cttctctctg     2820

ttaaagcaag caggagacgt ggaagaaaac cccggtccta tgctcgaggg agtgcaggtg     2880

gaaaccatct ccccaggaga cgggcgcacc ttccccaagc gcggccagac ctgcgtggtg     2940

cactacaccg ggatgcttga agatggaaag aaagttgatt cctcccggga cagaaacaag     3000

ccctttaagt ttatgctagg caagcaggag gtgatccgag gctgggaaga aggggttgcc     3060

cagatgagtg tgggtcagag agccaaactg actatatctc cagattatgc ctatggtgcc     3120

actgggcacc caggcatcat cccaccacat gccactctcg tcttcgatgt ggagcttcta     3180

aaactggaat ctggcggtgg atccggagtc gacggatttg gtgatgtcgg tgctcttgag     3240

agtttgaggg gaaatgcaga tttggcttac atcctgagca tggagccctg tggccactgc     3300

ctcattatca acaatgtgaa cttctgccgt gagtccgggc tccgcacccg cactggctcc     3360

aacatcgact gtgagaagtt gcggcgtcgc ttctcctcgc tgcatttcat ggtggaggtg     3420

aagggcgacc tgactgccaa gaaaatggtg ctggctttgc tggagctggc gcagcaggac     3480

cacggtgctc tggactgctg cgtggtggtc attctctctc acggctgtca ggccagccac     3540

ctgcagttcc caggggctgt ctacggcaca gatggatgcc ctgtgtcggt cgagaagatt     3600

gtgaacatct tcaatgggac cagctgcccc agcctgggag ggaagcccaa gctctttttc     3660

atccaggcct gtggtgggga gcagaaagac catgggtttg aggtggcctc cacttcccct     3720

gaagacgagt cccctggcag taaccccgag ccagatgcca ccccgttcca ggaaggtttg     3780

aggaccttcg accagctgga cgccatatct agtttgccca cacccagtga catctttgtg     3840

tcctactcta ctttcccagg ttttgtttcc tggagggacc ccaagagtgg ctcctggtac     3900

gttgagaccc tggacgacat ctttgagcag tgggctcact ctgaagacct gcagtccctc     3960

ctgcttaggg tcgctaatgc tgtttcggtg aaagggattt ataaacagat gcctggttgc     4020

tttaatttcc tccggaaaaa acttttcttt aaaacatcag tcgactatcc gtacgacgta     4080

ccagactacg cactcgacta a                                               4101


<210>  33
<211>  10348
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector (CART4: CAR4-tEGFR-iC9)

<400>  33
tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat       60

ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca      120

gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga      180

acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt      240

ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc      300

gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc      360

ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata      420

aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca      480

gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga      540

cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg      600

tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt      660

actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa      720

cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga      780

cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag      840

acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa      900

gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg      960

tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct     1020

taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga     1080

gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag     1140

acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc     1200

cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc     1260

cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg     1320

ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag     1380

ccctcactcc ttctctaggc gccggaatta gatctctcga ggttaacgaa ttctaccggg     1440

taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca     1500

cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc     1560

aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga     1620

agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc     1680

tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg     1740

gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg     1800

ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg     1860

gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct     1920

cattctccgg gcctttcgac ctgcagccca agccaccatg gagacagaca cactcctgct     1980

atgggtgctg ctgctctggg ttccaggttc cacaggtgac gacattgtga tgactcagag     2040

ccccgacagc ctggccgtct cactgggcga aagggtgacc atgaattgta aatcttctca     2100

gagcctgctg tacagtacaa accagaaaaa ttacctggcc tggtatcagc agaaacccgg     2160

ccagagccct aagctgctga tctattgggc aagtacccga gagtcaggag tgccagacag     2220

attctccggg tctggaagtg gcacagactt caccctgaca attagctccg tgcaggccga     2280

ggacgtggct gtctactatt gccagcagta ctatagctac cgaactttcg gcgggggaac     2340

caaactggaa atcaagggag gaggaggcag tggcggagga gggtcaggag gaggaggaag     2400

ccaggtgcag ctgcagcagt ccggaccaga ggtggtcaaa cccggcgcta gcgtcaaaat     2460

gtcctgtaag gcatctggct acactttcac ctcttatgtg attcactggg tcagacagaa     2520

gcctgggcag ggactggact ggatcgggta cattaaccca tataatgatg gaactgacta     2580

cgatgaaaag tttaaaggca aggccacact gacttccgac acctcaacaa gcactgctta     2640

tatggagctg tctagtctga ggtctgaaga cacagcagtg tactattgcg cccgcgagaa     2700

ggataactac gccactggcg cttggtttgc atattggggc caggggaccc tggtgacagt     2760

ctcatccgcg gccgcattcg tgccggtctt cctgccagcg aagcccacca cgacgccagc     2820

gccgcgacca ccaacaccgg cgcccaccat cgcgtcgcag cccctgtccc tgcgcccaga     2880

ggcgtgccgg ccagcggcgg ggggcgcagt gcacacgagg gggctggact tcgcctgtga     2940

tatctacatc tgggcgccct tggccgggac ttgtggggtc cttctcctgt cactggttat     3000

caccctttac tgcaaccaca ggaacaggag taagaggagc aggctcctgc acagtgacta     3060

catgaacatg actccccgcc gccccgggcc cacccgcaag cattaccagc cctatgcccc     3120

accacgcgac ttcgcagcct atcgctcccg tttctctgtt gttaaacggg gcagaaagaa     3180

gctcctgtat atattcaaac aaccatttat gagaccagta caaactactc aagaggaaga     3240

tggctgtagc tgccgatttc cagaagaaga agaaggagga tgtgaactga gagtgaagtt     3300

cagcaggagc gcagacgccc ccgcgtacca gcagggccag aaccagctct ataacgagct     3360

caatctagga cgaagagagg agtacgatgt tttggacaag agacgtggcc gggaccctga     3420

gatgggggga aagccgagaa ggaagaaccc tcaggaaggc ctgtacaatg aactgcagaa     3480

agataagatg gcggaggcct acagtgagat tgggatgaaa ggcgagcgcc ggaggggcaa     3540

ggggcacgat ggcctttacc agggtctcag tacagccacc aaggacacct acgacgccct     3600

tcacatgcag gccctgcccc ctcgcggatc cggagccacg aacttctctc tgttaaagca     3660

agcaggagac gtggaagaaa accccggtcc tatgcttctc ctggtgacaa gccttctgct     3720

ctgtgagtta ccacacccag cattcctcct gatcccacgc aaagtgtgta acggaatagg     3780

tattggtgaa tttaaagact cactctccat aaatgctacg aatattaaac acttcaaaaa     3840

ctgcacctcc atcagtggcg atctccacat cctgccggtg gcatttaggg gtgactcctt     3900

cacacatact cctcctctgg atccacagga actggatatt ctgaaaaccg taaaggaaat     3960

cacagggttt ttgctgattc aggcttggcc tgaaaacagg acggacctcc atgcctttga     4020

gaacctagaa atcatacgcg gcaggaccaa gcaacatggt cagttttctc ttgcagtcgt     4080

cagcctgaac ataacatcct tgggattacg ctccctcaag gagataagtg atggagatgt     4140

gataatttca ggaaacaaaa atttgtgcta tgcaaataca ataaactgga aaaaactgtt     4200

tgggacctcc ggtcagaaaa ccaaaattat aagcaacaga ggtgaaaaca gctgcaaggc     4260

cacaggccag gtctgccatg ccttgtgctc ccccgagggc tgctggggcc cggaacccag     4320

ggactgcgtc tcttgccgga atgtcagccg aggcagggaa tgcgtggaca agtgcaacct     4380

tctggagggt gagccaaggg agtttgtgga gaactctgag tgcatacagt gccacccaga     4440

gtgcctgcct caggccatga acatcacctg cacaggacgg ggaccagaca actgtatcca     4500

gtgtgcccac tacattgacg gcccccactg cgtcaagacc tgcccggcag gagtcatggg     4560

agaaaacaac accctggtct ggaagtacgc agacgccggc catgtgtgcc acctgtgcca     4620

tccaaactgc acctacggat gcactgggcc aggtcttgaa ggctgtccaa cgaatgggcc     4680

taagatcccg tccatcgcca ctgggatggt gggggccctc ctcttgctgc tggtggtggc     4740

cctggggatc ggcctcttca tgggatctgg agccacgaac ttctctctgt taaagcaagc     4800

aggagacgtg gaagaaaacc ccggtcctat gctcgaggga gtgcaggtgg aaaccatctc     4860

cccaggagac gggcgcacct tccccaagcg cggccagacc tgcgtggtgc actacaccgg     4920

gatgcttgaa gatggaaaga aagttgattc ctcccgggac agaaacaagc cctttaagtt     4980

tatgctaggc aagcaggagg tgatccgagg ctgggaagaa ggggttgccc agatgagtgt     5040

gggtcagaga gccaaactga ctatatctcc agattatgcc tatggtgcca ctgggcaccc     5100

aggcatcatc ccaccacatg ccactctcgt cttcgatgtg gagcttctaa aactggaatc     5160

tggcggtgga tccggagtcg acggatttgg tgatgtcggt gctcttgaga gtttgagggg     5220

aaatgcagat ttggcttaca tcctgagcat ggagccctgt ggccactgcc tcattatcaa     5280

caatgtgaac ttctgccgtg agtccgggct ccgcacccgc actggctcca acatcgactg     5340

tgagaagttg cggcgtcgct tctcctcgct gcatttcatg gtggaggtga agggcgacct     5400

gactgccaag aaaatggtgc tggctttgct ggagctggcg cagcaggacc acggtgctct     5460

ggactgctgc gtggtggtca ttctctctca cggctgtcag gccagccacc tgcagttccc     5520

aggggctgtc tacggcacag atggatgccc tgtgtcggtc gagaagattg tgaacatctt     5580

caatgggacc agctgcccca gcctgggagg gaagcccaag ctctttttca tccaggcctg     5640

tggtggggag cagaaagacc atgggtttga ggtggcctcc acttcccctg aagacgagtc     5700

ccctggcagt aaccccgagc cagatgccac cccgttccag gaaggtttga ggaccttcga     5760

ccagctggac gccatatcta gtttgcccac acccagtgac atctttgtgt cctactctac     5820

tttcccaggt tttgtttcct ggagggaccc caagagtggc tcctggtacg ttgagaccct     5880

ggacgacatc tttgagcagt gggctcactc tgaagacctg cagtccctcc tgcttagggt     5940

cgctaatgct gtttcggtga aagggattta taaacagatg cctggttgct ttaatttcct     6000

ccggaaaaaa cttttcttta aaacatcagt cgactatccg tacgacgtac cagactacgc     6060

actcgactaa acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt     6120

aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct     6180

attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt     6240

tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac     6300

gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct     6360

ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca     6420

ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt     6480

ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc     6540

ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct     6600

cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg     6660

cctatcgata aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc     6720

cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaataca     6780

taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca gaatatgggc     6840

caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc     6900

cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc     6960

ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct     7020

tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc     7080

gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt gtatccaata aaccctcttg     7140

cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac     7200

tacccgtcag cgggggtctt tcatgggtaa cagtttcttg aagttggaga acaacattct     7260

gagggtagga gtcgaatatt aagtaatcct gactcaatta gccactgttt tgaatccaca     7320

tactccaata ctcctgaaat agttcattat ggacagcgca gaagagctgg ggagaattaa     7380

ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca     7440

caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact     7500

cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct     7560

gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc     7620

ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca     7680

ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg     7740

agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca     7800

taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa     7860

cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc     7920

tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc     7980

gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct     8040

gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg     8100

tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag     8160

gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta     8220

cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg     8280

aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt     8340

tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt     8400

ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag     8460

attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat     8520

ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc     8580

tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat     8640

aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc     8700

acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag     8760

aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag     8820

agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt     8880

ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg     8940

agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt     9000

tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc     9060

tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc     9120

attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa     9180

taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg     9240

aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc     9300

caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag     9360

gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt     9420

cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt     9480

tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc     9540

acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac     9600

gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct     9660

cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg     9720

cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat     9780

tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata     9840

ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg     9900

ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg     9960

ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggcgcaag gaatggtgca    10020

tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg    10080

aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg    10140

atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg    10200

tagaggcgat tagtccaatt tgttaaagac aggatatcag tggtccaggc tctagttttg    10260

actcaacaat atcaccagct gaagcctata gagtacgagc catagataaa ataaaagatt    10320

ttatttagtc tccagaaaaa ggggggaa                                       10348


<210>  34
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  First coding sequence (VH for TCR V-beta)

<400>  34

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Gly Lys Val Thr Leu Thr Cys Lys Ala Ser Gln Asp Ile Asn Lys Tyr 
            20                  25                  30          


Ile Ala Trp Tyr Gln His Lys Pro Gly Lys Gly Pro Arg Leu Leu Ile 
        35                  40                  45              


His Tyr Thr Ser Thr Leu Gln Pro Gly Ile Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Arg Asp Tyr Ser Phe Ser Ile Ser Asn Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Val Ala Thr Tyr Tyr Cys Leu Gln Tyr Asp Asn Leu Arg Thr 
                85                  90                  95      


Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Thr Asp 
            100                 105                 


<210>  35
<211>  327
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  First coding sequence (VH for TCR V-beta)

<400>  35
gacatccaga tgacacagag ccctagcagc ctgtctgcct ctctcggcgg aaaagtgacc       60

ctgacatgca aggccagcca ggacatcaac aagtatatcg cctggtatca gcacaagccc      120

ggcaagggac ctagactgct gatccactac accagcacac tgcagcctgg catccccagc      180

agattttctg gcagcggctc cggcagagac tacagcttca gcatcagcaa cctggaacct      240

gaggacgtgg ccacctacta ctgcctgcag tacgacaacc tgcggacctt tggcggcgga      300

acaaagctgg aaatcaagcg gacagat                                          327


<210>  36
<211>  10336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector (CARTVb7.1: CARTVb7.1-tEGFR-iC9)

<400>  36
tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat       60

ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca      120

gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga      180

acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt      240

ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc      300

gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc      360

ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata      420

aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca      480

gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga      540

cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg      600

tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt      660

actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa      720

cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga      780

cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag      840

acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa      900

gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg      960

tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct     1020

taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga     1080

gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag     1140

acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc     1200

cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc     1260

cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg     1320

ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag     1380

ccctcactcc ttctctaggc gccggaatta gatctctcga ggttaacgaa ttctaccggg     1440

taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca     1500

cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc     1560

aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga     1620

agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc     1680

tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg     1740

gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg     1800

ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg     1860

gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct     1920

cattctccgg gcctttcgac ctgcagccca agccaccatg gctctgcctg ttacagctct     1980

gctgctgcct ctggctctgc ttctgcatgc cgccagacct gacatccaga tgacacagag     2040

ccctagcagc ctgtctgcct ctctcggcgg aaaagtgacc ctgacatgca aggccagcca     2100

ggacatcaac aagtatatcg cctggtatca gcacaagccc ggcaagggac ctagactgct     2160

gatccactac accagcacac tgcagcctgg catccccagc agattttctg gcagcggctc     2220

cggcagagac tacagcttca gcatcagcaa cctggaacct gaggacgtgg ccacctacta     2280

ctgcctgcag tacgacaacc tgcggacctt tggcggcgga acaaagctgg aaatcaagcg     2340

gacagatggc ggaggcggat caggcggcgg aggaagcggt ggcggaggat ctcaagttca     2400

gctgcaacag cctggcgccg agcttgtgaa acctggcgcc tctgtgaaga tgagctgcaa     2460

ggcctccggc tacaccttca ccagatactg gatcacctgg gtcaagcaga ggcctggaca     2520

gggactcgag tggatcggcg atatctatcc tggctccggc ttcaccaagt acaacgagaa     2580

gttcaagagc aaggccacac tgaccgtgga caccagcagc agcacagcct acatgcagct     2640

gtctagcctg accagcgagg acagcgccgt gtactactgt gctagagaag gcggcaacta     2700

ctggtacttc gacgtgtggg gcaccggcac cacagtgaca gttagttctg cggccgcggc     2760

cgcattcgtg ccggtcttcc tgccagcgaa gcccaccacg acgccagcgc cgcgaccacc     2820

aacaccggcg cccaccatcg cgtcgcagcc cctgtccctg cgcccagagg cgtgccggcc     2880

agcggcgggg ggcgcagtgc acacgagggg gctggacttc gcctgtgata tctacatctg     2940

ggcgcccttg gccgggactt gtggggtcct tctcctgtca ctggttatca ccctttactg     3000

caaccacagg aacaggagta agaggagcag gctcctgcac agtgactaca tgaacatgac     3060

tccccgccgc cccgggccca cccgcaagca ttaccagccc tatgccccac cacgcgactt     3120

cgcagcctat cgctcccgtt tctctgttgt taaacggggc agaaagaagc tcctgtatat     3180

attcaaacaa ccatttatga gaccagtaca aactactcaa gaggaagatg gctgtagctg     3240

ccgatttcca gaagaagaag aaggaggatg tgaactgaga gtgaagttca gcaggagcgc     3300

agacgccccc gcgtaccagc agggccagaa ccagctctat aacgagctca atctaggacg     3360

aagagaggag tacgatgttt tggacaagag acgtggccgg gaccctgaga tggggggaaa     3420

gccgagaagg aagaaccctc aggaaggcct gtacaatgaa ctgcagaaag ataagatggc     3480

ggaggcctac agtgagattg ggatgaaagg cgagcgccgg aggggcaagg ggcacgatgg     3540

cctttaccag ggtctcagta cagccaccaa ggacacctac gacgcccttc acatgcaggc     3600

cctgccccct cgcggatccg gagccacgaa cttctctctg ttaaagcaag caggagacgt     3660

ggaagaaaac cccggtccta tgcttctcct ggtgacaagc cttctgctct gtgagttacc     3720

acacccagca ttcctcctga tcccacgcaa agtgtgtaac ggaataggta ttggtgaatt     3780

taaagactca ctctccataa atgctacgaa tattaaacac ttcaaaaact gcacctccat     3840

cagtggcgat ctccacatcc tgccggtggc atttaggggt gactccttca cacatactcc     3900

tcctctggat ccacaggaac tggatattct gaaaaccgta aaggaaatca cagggttttt     3960

gctgattcag gcttggcctg aaaacaggac ggacctccat gcctttgaga acctagaaat     4020

catacgcggc aggaccaagc aacatggtca gttttctctt gcagtcgtca gcctgaacat     4080

aacatccttg ggattacgct ccctcaagga gataagtgat ggagatgtga taatttcagg     4140

aaacaaaaat ttgtgctatg caaatacaat aaactggaaa aaactgtttg ggacctccgg     4200

tcagaaaacc aaaattataa gcaacagagg tgaaaacagc tgcaaggcca caggccaggt     4260

ctgccatgcc ttgtgctccc ccgagggctg ctggggcccg gaacccaggg actgcgtctc     4320

ttgccggaat gtcagccgag gcagggaatg cgtggacaag tgcaaccttc tggagggtga     4380

gccaagggag tttgtggaga actctgagtg catacagtgc cacccagagt gcctgcctca     4440

ggccatgaac atcacctgca caggacgggg accagacaac tgtatccagt gtgcccacta     4500

cattgacggc ccccactgcg tcaagacctg cccggcagga gtcatgggag aaaacaacac     4560

cctggtctgg aagtacgcag acgccggcca tgtgtgccac ctgtgccatc caaactgcac     4620

ctacggatgc actgggccag gtcttgaagg ctgtccaacg aatgggccta agatcccgtc     4680

catcgccact gggatggtgg gggccctcct cttgctgctg gtggtggccc tggggatcgg     4740

cctcttcatg ggatctggag ccacgaactt ctctctgtta aagcaagcag gagacgtgga     4800

agaaaacccc ggtcctatgc tcgagggagt gcaggtggaa accatctccc caggagacgg     4860

gcgcaccttc cccaagcgcg gccagacctg cgtggtgcac tacaccggga tgcttgaaga     4920

tggaaagaaa gttgattcct cccgggacag aaacaagccc tttaagttta tgctaggcaa     4980

gcaggaggtg atccgaggct gggaagaagg ggttgcccag atgagtgtgg gtcagagagc     5040

caaactgact atatctccag attatgccta tggtgccact gggcacccag gcatcatccc     5100

accacatgcc actctcgtct tcgatgtgga gcttctaaaa ctggaatctg gcggtggatc     5160

cggagtcgac ggatttggtg atgtcggtgc tcttgagagt ttgaggggaa atgcagattt     5220

ggcttacatc ctgagcatgg agccctgtgg ccactgcctc attatcaaca atgtgaactt     5280

ctgccgtgag tccgggctcc gcacccgcac tggctccaac atcgactgtg agaagttgcg     5340

gcgtcgcttc tcctcgctgc atttcatggt ggaggtgaag ggcgacctga ctgccaagaa     5400

aatggtgctg gctttgctgg agctggcgca gcaggaccac ggtgctctgg actgctgcgt     5460

ggtggtcatt ctctctcacg gctgtcaggc cagccacctg cagttcccag gggctgtcta     5520

cggcacagat ggatgccctg tgtcggtcga gaagattgtg aacatcttca atgggaccag     5580

ctgccccagc ctgggaggga agcccaagct ctttttcatc caggcctgtg gtggggagca     5640

gaaagaccat gggtttgagg tggcctccac ttcccctgaa gacgagtccc ctggcagtaa     5700

ccccgagcca gatgccaccc cgttccagga aggtttgagg accttcgacc agctggacgc     5760

catatctagt ttgcccacac ccagtgacat ctttgtgtcc tactctactt tcccaggttt     5820

tgtttcctgg agggacccca agagtggctc ctggtacgtt gagaccctgg acgacatctt     5880

tgagcagtgg gctcactctg aagacctgca gtccctcctg cttagggtcg ctaatgctgt     5940

ttcggtgaaa gggatttata aacagatgcc tggttgcttt aatttcctcc ggaaaaaact     6000

tttctttaaa acatcagtcg actatccgta cgacgtacca gactacgcac tcgactaaac     6060

aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct     6120

ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt     6180

atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg     6240

tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact     6300

ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct     6360

attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg     6420

ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc     6480

gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc     6540

aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt     6600

cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tatcgataaa     6660

ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt     6720

tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaatacata actgagaata     6780

gagaagttca gatcaaggtt aggaacagag agacagcaga atatgggcca aacaggatat     6840

ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt     6900

cccgccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga     6960

aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg     7020

cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcctc     7080

cgatagactg cgtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg     7140

acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg     7200

ggggtctttc atgggtaaca gtttcttgaa gttggagaac aacattctga gggtaggagt     7260

cgaatattaa gtaatcctga ctcaattagc cactgttttg aatccacata ctccaatact     7320

cctgaaatag ttcattatgg acagcgcaga agagctgggg agaattaatt cgtaatcatg     7380

gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc     7440

cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc     7500

gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat     7560

cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac     7620

tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt     7680

aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca     7740

gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc     7800

ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact     7860

ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct     7920

gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag     7980

ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca     8040

cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa     8100

cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc     8160

gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag     8220

aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg     8280

tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca     8340

gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc     8400

tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag     8460

gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata     8520

tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat     8580

ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg     8640

ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc     8700

tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc     8760

aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc     8820

gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc     8880

gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc     8940

ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa     9000

gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat     9060

gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata     9120

gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca     9180

tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag     9240

gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc     9300

agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc     9360

aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata     9420

ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta     9480

gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta     9540

agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg     9600

tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt     9660

cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg     9720

tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt     9780

gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg     9840

ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct     9900

attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg     9960

gttttcccag tcacgacgtt gtaaaacgac ggcgcaagga atggtgcatg caaggagatg    10020

gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc    10080

atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca    10140

gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggcgatta    10200

gtccaatttg ttaaagacag gatatcagtg gtccaggctc tagttttgac tcaacaatat    10260

caccagctga agcctataga gtacgagcca tagataaaat aaaagatttt atttagtctc    10320

cagaaaaagg ggggaa                                                    10336


