                         SEQUENCE LISTING

<110>  JANSSEN PHARMACEUTICA NV
 
<120>  EXPRESSION SYSTEMS FOR THE ALPHA2ALPHA5BETA2 NICOTINIC 
       ACETYLCHOLINE RECEPTOR AND METHODS OF USE THEREOF

<130>  PRD4128WOPCT1

<150>  US 63/178,813
<151>  2021-04-23

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  529
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Gly Pro Ser Cys Pro Val Phe Leu Ser Phe Thr Lys Leu Ser Leu 
1               5                   10                  15      


Trp Trp Leu Leu Leu Thr Pro Ala Gly Gly Glu Glu Ala Lys Arg Pro 
            20                  25                  30          


Pro Pro Arg Ala Pro Gly Asp Pro Leu Ser Ser Pro Ser Pro Thr Ala 
        35                  40                  45              


Leu Pro Gln Gly Gly Ser His Thr Glu Thr Glu Asp Arg Leu Phe Lys 
    50                  55                  60                  


His Leu Phe Arg Gly Tyr Asn Arg Trp Ala Arg Pro Val Pro Asn Thr 
65                  70                  75                  80  


Ser Asp Val Val Ile Val Arg Phe Gly Leu Ser Ile Ala Gln Leu Ile 
                85                  90                  95      


Asp Val Asp Glu Lys Asn Gln Met Met Thr Thr Asn Val Trp Leu Lys 
            100                 105                 110         


Gln Glu Trp Ser Asp Tyr Lys Leu Arg Trp Asn Pro Thr Asp Phe Gly 
        115                 120                 125             


Asn Ile Thr Ser Leu Arg Val Pro Ser Glu Met Ile Trp Ile Pro Asp 
    130                 135                 140                 


Ile Val Leu Tyr Asn Asn Ala Asp Gly Glu Phe Ala Val Thr His Met 
145                 150                 155                 160 


Thr Lys Ala His Leu Phe Ser Thr Gly Thr Val His Trp Val Pro Pro 
                165                 170                 175     


Ala Ile Tyr Lys Ser Ser Cys Ser Ile Asp Val Thr Phe Phe Pro Phe 
            180                 185                 190         


Asp Gln Gln Asn Cys Lys Met Lys Phe Gly Ser Trp Thr Tyr Asp Lys 
        195                 200                 205             


Ala Lys Ile Asp Leu Glu Gln Met Glu Gln Thr Val Asp Leu Lys Asp 
    210                 215                 220                 


Tyr Trp Glu Ser Gly Glu Trp Ala Ile Val Asn Ala Thr Gly Thr Tyr 
225                 230                 235                 240 


Asn Ser Lys Lys Tyr Asp Cys Cys Ala Glu Ile Tyr Pro Asp Val Thr 
                245                 250                 255     


Tyr Ala Phe Val Ile Arg Arg Leu Pro Leu Phe Tyr Thr Ile Asn Leu 
            260                 265                 270         


Ile Ile Pro Cys Leu Leu Ile Ser Cys Leu Thr Val Leu Val Phe Tyr 
        275                 280                 285             


Leu Pro Ser Asp Cys Gly Glu Lys Ile Thr Leu Cys Ile Ser Val Leu 
    290                 295                 300                 


Leu Ser Leu Thr Val Phe Leu Leu Leu Ile Thr Glu Ile Ile Pro Ser 
305                 310                 315                 320 


Thr Ser Leu Val Ile Pro Leu Ile Gly Glu Tyr Leu Leu Phe Thr Met 
                325                 330                 335     


Ile Phe Val Thr Leu Ser Ile Val Ile Thr Val Phe Val Leu Asn Val 
            340                 345                 350         


His His Arg Ser Pro Ser Thr His Thr Met Pro His Trp Val Arg Gly 
        355                 360                 365             


Ala Leu Leu Gly Cys Val Pro Arg Trp Leu Leu Met Asn Arg Pro Pro 
    370                 375                 380                 


Pro Pro Val Glu Leu Cys His Pro Leu Arg Leu Lys Leu Ser Pro Ser 
385                 390                 395                 400 


Tyr His Trp Leu Glu Ser Asn Val Asp Ala Glu Glu Arg Glu Val Val 
                405                 410                 415     


Val Glu Glu Glu Asp Arg Trp Ala Cys Ala Gly His Val Ala Pro Ser 
            420                 425                 430         


Val Gly Thr Leu Cys Ser His Gly His Leu His Ser Gly Ala Ser Gly 
        435                 440                 445             


Pro Lys Ala Glu Ala Leu Leu Gln Glu Gly Glu Leu Leu Leu Ser Pro 
    450                 455                 460                 


His Met Gln Lys Ala Leu Glu Gly Val His Tyr Ile Ala Asp His Leu 
465                 470                 475                 480 


Arg Ser Glu Asp Ala Asp Ser Ser Val Lys Glu Asp Trp Lys Tyr Val 
                485                 490                 495     


Ala Met Val Ile Asp Arg Ile Phe Leu Trp Leu Phe Ile Ile Val Cys 
            500                 505                 510         


Phe Leu Gly Thr Ile Gly Leu Phe Leu Pro Pro Phe Leu Ala Gly Met 
        515                 520                 525             


Ile 
    


<210>  2
<211>  468
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Ala Ala Arg Gly Ser Gly Pro Arg Ala Leu Arg Leu Leu Leu Leu 
1               5                   10                  15      


Val Gln Leu Val Ala Gly Arg Cys Gly Leu Ala Gly Ala Ala Gly Gly 
            20                  25                  30          


Ala Gln Arg Gly Leu Ser Glu Pro Ser Ser Ile Ala Lys His Glu Asp 
        35                  40                  45              


Ser Leu Leu Lys Asp Leu Phe Gln Asp Tyr Glu Arg Trp Val Arg Pro 
    50                  55                  60                  


Val Glu His Leu Asn Asp Lys Ile Lys Ile Lys Phe Gly Leu Ala Ile 
65                  70                  75                  80  


Ser Gln Leu Val Asp Val Asp Glu Lys Asn Gln Leu Met Thr Thr Asn 
                85                  90                  95      


Val Trp Leu Lys Gln Glu Trp Ile Asp Val Lys Leu Arg Trp Asn Pro 
            100                 105                 110         


Asp Asp Tyr Gly Gly Ile Lys Val Ile Arg Val Pro Ser Asp Ser Val 
        115                 120                 125             


Trp Thr Pro Asp Ile Val Leu Phe Asp Asn Ala Asp Gly Arg Phe Glu 
    130                 135                 140                 


Gly Thr Ser Thr Lys Thr Val Ile Arg Tyr Asn Gly Thr Val Thr Trp 
145                 150                 155                 160 


Thr Pro Pro Ala Asn Tyr Lys Ser Ser Cys Thr Ile Asp Val Thr Phe 
                165                 170                 175     


Phe Pro Phe Asp Leu Gln Asn Cys Ser Met Lys Phe Gly Ser Trp Thr 
            180                 185                 190         


Tyr Asp Gly Ser Gln Val Asp Ile Ile Leu Glu Asp Gln Asp Val Asp 
        195                 200                 205             


Lys Arg Asp Phe Phe Asp Asn Gly Glu Trp Glu Ile Val Ser Ala Thr 
    210                 215                 220                 


Gly Ser Lys Gly Asn Arg Thr Asp Ser Cys Cys Trp Tyr Pro Tyr Val 
225                 230                 235                 240 


Thr Tyr Ser Phe Val Ile Lys Arg Leu Pro Leu Phe Tyr Thr Leu Phe 
                245                 250                 255     


Leu Ile Ile Pro Cys Ile Gly Leu Ser Phe Leu Thr Val Leu Val Phe 
            260                 265                 270         


Tyr Leu Pro Ser Asn Glu Gly Glu Lys Ile Cys Leu Cys Thr Ser Val 
        275                 280                 285             


Leu Val Ser Leu Thr Val Phe Leu Leu Val Ile Glu Glu Ile Ile Pro 
    290                 295                 300                 


Ser Ser Ser Lys Val Ile Pro Leu Ile Gly Glu Tyr Leu Val Phe Thr 
305                 310                 315                 320 


Met Ile Phe Val Thr Leu Ser Ile Met Val Thr Val Phe Ala Ile Asn 
                325                 330                 335     


Ile His His Arg Ser Ser Ser Thr His Asn Ala Met Ala Pro Leu Val 
            340                 345                 350         


Arg Lys Ile Phe Leu His Thr Leu Pro Lys Leu Leu Cys Met Arg Ser 
        355                 360                 365             


His Val Asp Arg Tyr Phe Thr Gln Lys Glu Glu Thr Glu Ser Gly Ser 
    370                 375                 380                 


Gly Pro Lys Ser Ser Arg Asn Thr Leu Glu Ala Ala Leu Asp Ser Ile 
385                 390                 395                 400 


Arg Tyr Ile Thr Arg His Ile Met Lys Glu Asn Asp Val Arg Glu Val 
                405                 410                 415     


Val Glu Asp Trp Lys Phe Ile Ala Gln Val Leu Asp Arg Met Phe Leu 
            420                 425                 430         


Trp Thr Phe Leu Phe Val Ser Ile Val Gly Ser Leu Gly Leu Phe Val 
        435                 440                 445             


Pro Val Ile Tyr Lys Trp Ala Asn Ile Leu Ile Pro Val His Ile Gly 
    450                 455                 460                 


Asn Ala Asn Lys 
465             


<210>  3
<211>  502
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Ala Arg Arg Cys Gly Pro Val Ala Leu Leu Leu Gly Phe Gly Leu 
1               5                   10                  15      


Leu Arg Leu Cys Ser Gly Val Trp Gly Thr Asp Thr Glu Glu Arg Leu 
            20                  25                  30          


Val Glu His Leu Leu Asp Pro Ser Arg Tyr Asn Lys Leu Ile Arg Pro 
        35                  40                  45              


Ala Thr Asn Gly Ser Glu Leu Val Thr Val Gln Leu Met Val Ser Leu 
    50                  55                  60                  


Ala Gln Leu Ile Ser Val His Glu Arg Glu Gln Ile Met Thr Thr Asn 
65                  70                  75                  80  


Val Trp Leu Thr Gln Glu Trp Glu Asp Tyr Arg Leu Thr Trp Lys Pro 
                85                  90                  95      


Glu Glu Phe Asp Asn Met Lys Lys Val Arg Leu Pro Ser Lys His Ile 
            100                 105                 110         


Trp Leu Pro Asp Val Val Leu Tyr Asn Asn Ala Asp Gly Met Tyr Glu 
        115                 120                 125             


Val Ser Phe Tyr Ser Asn Ala Val Val Ser Tyr Asp Gly Ser Ile Phe 
    130                 135                 140                 


Trp Leu Pro Pro Ala Ile Tyr Lys Ser Ala Cys Lys Ile Glu Val Lys 
145                 150                 155                 160 


His Phe Pro Phe Asp Gln Gln Asn Cys Thr Met Lys Phe Arg Ser Trp 
                165                 170                 175     


Thr Tyr Asp Arg Thr Glu Ile Asp Leu Val Leu Lys Ser Glu Val Ala 
            180                 185                 190         


Ser Leu Asp Asp Phe Thr Pro Ser Gly Glu Trp Asp Ile Val Ala Leu 
        195                 200                 205             


Pro Gly Arg Arg Asn Glu Asn Pro Asp Asp Ser Thr Tyr Val Asp Ile 
    210                 215                 220                 


Thr Tyr Asp Phe Ile Ile Arg Arg Lys Pro Leu Phe Tyr Thr Ile Asn 
225                 230                 235                 240 


Leu Ile Ile Pro Cys Val Leu Ile Thr Ser Leu Ala Ile Leu Val Phe 
                245                 250                 255     


Tyr Leu Pro Ser Asp Cys Gly Glu Lys Met Thr Leu Cys Ile Ser Val 
            260                 265                 270         


Leu Leu Ala Leu Thr Val Phe Leu Leu Leu Ile Ser Lys Ile Val Pro 
        275                 280                 285             


Pro Thr Ser Leu Asp Val Pro Leu Val Gly Lys Tyr Leu Met Phe Thr 
    290                 295                 300                 


Met Val Leu Val Thr Phe Ser Ile Val Thr Ser Val Cys Val Leu Asn 
305                 310                 315                 320 


Val His His Arg Ser Pro Thr Thr His Thr Met Ala Pro Trp Val Lys 
                325                 330                 335     


Val Val Phe Leu Glu Lys Leu Pro Ala Leu Leu Phe Met Gln Gln Pro 
            340                 345                 350         


Arg His His Cys Ala Arg Gln Arg Leu Arg Leu Arg Arg Arg Gln Arg 
        355                 360                 365             


Glu Arg Glu Gly Ala Gly Ala Leu Phe Phe Arg Glu Ala Pro Gly Ala 
    370                 375                 380                 


Asp Ser Cys Thr Cys Phe Val Asn Arg Ala Ser Val Gln Gly Leu Ala 
385                 390                 395                 400 


Gly Ala Phe Gly Ala Glu Pro Ala Pro Val Ala Gly Pro Gly Arg Ser 
                405                 410                 415     


Gly Glu Pro Cys Gly Cys Gly Leu Arg Glu Ala Val Asp Gly Val Arg 
            420                 425                 430         


Phe Ile Ala Asp His Met Arg Ser Glu Asp Asp Asp Gln Ser Val Ser 
        435                 440                 445             


Glu Asp Trp Lys Tyr Val Ala Met Val Ile Asp Arg Leu Phe Leu Trp 
    450                 455                 460                 


Ile Phe Val Phe Val Cys Val Phe Gly Thr Ile Gly Met Phe Leu Gln 
465                 470                 475                 480 


Pro Leu Phe Gln Asn Tyr Thr Thr Thr Thr Phe Leu His Ser Asp His 
                485                 490                 495     


Ser Ala Pro Ser Ser Lys 
            500         


<210>  4
<211>  156
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Ala Gly Trp Pro Gly Ala Gly Pro Leu Cys Val Leu Gly Gly Ala 
1               5                   10                  15      


Ala Leu Gly Val Cys Leu Ala Gly Val Ala Gly Gln Leu Val Glu Pro 
            20                  25                  30          


Ser Thr Ala Pro Pro Lys Pro Lys Pro Pro Pro Leu Thr Lys Glu Thr 
        35                  40                  45              


Val Val Phe Trp Asp Met Arg Leu Trp His Val Val Gly Ile Phe Ser 
    50                  55                  60                  


Leu Phe Val Leu Ser Ile Ile Ile Thr Leu Cys Cys Val Phe Asn Cys 
65                  70                  75                  80  


Arg Val Pro Arg Thr Arg Lys Glu Ile Glu Ala Arg Tyr Leu Gln Arg 
                85                  90                  95      


Lys Ala Ala Lys Met Tyr Thr Asp Lys Leu Glu Thr Val Pro Pro Leu 
            100                 105                 110         


Asn Glu Leu Thr Glu Val Pro Gly Glu Asp Lys Lys Lys Lys Lys Lys 
        115                 120                 125             


Lys Lys Lys Asp Ser Val Asp Thr Val Ala Ile Lys Val Glu Glu Asp 
    130                 135                 140                 


Glu Lys Asn Glu Ala Lys Lys Lys Lys Gly Glu Lys 
145                 150                 155     


<210>  5
<211>  166
<212>  PRT
<213>  Homo sapiens

<400>  5

Met Thr Ala Gly Thr Val Val Ile Thr Gly Gly Ile Leu Ala Thr Val 
1               5                   10                  15      


Ile Leu Leu Cys Ile Ile Ala Val Leu Cys Tyr Cys Arg Leu Gln Tyr 
            20                  25                  30          


Tyr Cys Cys Lys Lys Asp Glu Ser Glu Glu Asp Glu Glu Glu Pro Asp 
        35                  40                  45              


Phe Ala Val His Ser His Leu Pro Pro Leu His Ser Asn Arg Asn Leu 
    50                  55                  60                  


Val Leu Thr Asn Gly Pro Ala Leu Tyr Pro Thr Ala Ser Thr Ser Phe 
65                  70                  75                  80  


Ser Gln Lys Ser Pro Gln Ala Arg Ala Leu Cys Arg Ser Cys Ser His 
                85                  90                  95      


Cys Glu Pro Pro Thr Phe Phe Leu Gln Glu Pro Pro Glu Glu Glu Glu 
            100                 105                 110         


Asp Val Leu Asn Gly Gly Glu Arg Val Leu Tyr Lys Ser Val Ser Gln 
        115                 120                 125             


Glu Asp Val Glu Leu Pro Pro Gly Gly Phe Gly Gly Leu Gln Ala Leu 
    130                 135                 140                 


Asn Pro Asn Arg Leu Ser Ala Met Arg Glu Ala Phe Ala Arg Ser Arg 
145                 150                 155                 160 


Ser Ile Ser Thr Asp Val 
                165     


<210>  6
<211>  171
<212>  PRT
<213>  Homo sapiens

<400>  6

Met Ala Lys Phe Val Ile Arg Pro Ala Thr Ala Ala Asp Cys Ser Asp 
1               5                   10                  15      


Ile Leu Arg Leu Ile Lys Glu Leu Ala Lys Tyr Glu Tyr Met Glu Glu 
            20                  25                  30          


Gln Val Ile Leu Thr Glu Lys Asp Leu Leu Glu Asp Gly Phe Gly Glu 
        35                  40                  45              


His Pro Phe Tyr His Cys Leu Val Ala Glu Val Pro Lys Glu His Trp 
    50                  55                  60                  


Thr Pro Glu Gly His Ser Ile Val Gly Phe Ala Met Tyr Tyr Phe Thr 
65                  70                  75                  80  


Tyr Asp Pro Trp Ile Gly Lys Leu Leu Tyr Leu Glu Asp Phe Phe Val 
                85                  90                  95      


Met Ser Asp Tyr Arg Gly Phe Gly Ile Gly Ser Glu Ile Leu Lys Asn 
            100                 105                 110         


Leu Ser Gln Val Ala Met Arg Cys Arg Cys Ser Ser Met His Phe Leu 
        115                 120                 125             


Val Ala Glu Trp Asn Glu Pro Ser Ile Asn Phe Tyr Lys Arg Arg Gly 
    130                 135                 140                 


Ala Ser Asp Leu Ser Ser Glu Glu Gly Trp Arg Leu Phe Lys Ile Asp 
145                 150                 155                 160 


Lys Glu Tyr Leu Leu Lys Met Ala Thr Glu Glu 
                165                 170     


<210>  7
<211>  630
<212>  PRT
<213>  Homo sapiens

<400>  7

Met Ala Ala Lys Thr Pro Ser Ser Glu Glu Ser Gly Leu Pro Lys Leu 
1               5                   10                  15      


Pro Val Pro Pro Leu Gln Gln Thr Leu Ala Thr Tyr Leu Gln Cys Met 
            20                  25                  30          


Arg His Leu Val Ser Glu Glu Gln Phe Arg Lys Ser Gln Ala Ile Val 
        35                  40                  45              


Gln Gln Phe Gly Ala Pro Gly Gly Leu Gly Glu Thr Leu Gln Gln Lys 
    50                  55                  60                  


Leu Leu Glu Arg Gln Glu Lys Thr Ala Asn Trp Val Ser Glu Tyr Trp 
65                  70                  75                  80  


Leu Asn Asp Met Tyr Leu Asn Asn Arg Leu Ala Leu Pro Val Asn Ser 
                85                  90                  95      


Ser Pro Ala Val Ile Phe Ala Arg Gln His Phe Pro Gly Thr Asp Asp 
            100                 105                 110         


Gln Leu Arg Phe Ala Ala Ser Leu Ile Ser Gly Val Leu Ser Tyr Lys 
        115                 120                 125             


Ala Leu Leu Asp Ser His Ser Ile Pro Thr Asp Cys Ala Lys Gly Gln 
    130                 135                 140                 


Leu Ser Gly Gln Pro Leu Cys Met Lys Gln Tyr Tyr Gly Leu Phe Ser 
145                 150                 155                 160 


Ser Tyr Arg Leu Pro Gly His Thr Gln Asp Thr Leu Val Ala Gln Asn 
                165                 170                 175     


Ser Ser Ile Met Pro Glu Pro Glu His Val Ile Val Ala Cys Cys Asn 
            180                 185                 190         


Gln Phe Phe Val Leu Asp Val Val Ile Asn Phe Arg Arg Leu Ser Glu 
        195                 200                 205             


Gly Asp Leu Phe Thr Gln Leu Arg Lys Ile Val Lys Met Ala Ser Asn 
    210                 215                 220                 


Glu Asp Glu Arg Leu Pro Pro Ile Gly Leu Leu Thr Ser Asp Gly Arg 
225                 230                 235                 240 


Ser Glu Trp Ala Glu Ala Arg Thr Val Leu Val Lys Asp Ser Thr Asn 
                245                 250                 255     


Arg Asp Ser Leu Asp Met Ile Glu Arg Cys Ile Cys Leu Val Cys Leu 
            260                 265                 270         


Asp Ala Pro Gly Gly Val Glu Leu Ser Asp Thr His Arg Ala Leu Gln 
        275                 280                 285             


Leu Leu His Gly Gly Gly Tyr Ser Lys Asn Gly Ala Asn Arg Trp Tyr 
    290                 295                 300                 


Asp Lys Ser Leu Gln Phe Val Val Gly Arg Asp Gly Thr Cys Gly Val 
305                 310                 315                 320 


Val Cys Glu His Ser Pro Phe Asp Gly Ile Val Leu Val Gln Cys Thr 
                325                 330                 335     


Glu His Leu Leu Lys His Met Thr Gln Ser Ser Arg Lys Leu Ile Arg 
            340                 345                 350         


Ala Asp Ser Val Ser Glu Leu Pro Ala Pro Arg Arg Leu Arg Trp Lys 
        355                 360                 365             


Cys Ser Pro Glu Ile Gln Gly His Leu Ala Ser Ser Ala Glu Lys Leu 
    370                 375                 380                 


Gln Arg Ile Val Lys Asn Leu Asp Phe Ile Val Tyr Lys Phe Asp Asn 
385                 390                 395                 400 


Tyr Gly Lys Thr Phe Ile Lys Lys Gln Lys Cys Ser Pro Asp Ala Phe 
                405                 410                 415     


Ile Gln Val Ala Leu Gln Leu Ala Phe Tyr Arg Leu His Arg Arg Leu 
            420                 425                 430         


Val Pro Thr Tyr Glu Ser Ala Ser Ile Arg Arg Phe Gln Glu Gly Arg 
        435                 440                 445             


Val Asp Asn Ile Arg Ser Ala Thr Pro Glu Ala Leu Ala Phe Val Arg 
    450                 455                 460                 


Ala Val Thr Asp His Lys Ala Ala Val Pro Ala Ser Glu Lys Leu Leu 
465                 470                 475                 480 


Leu Leu Lys Asp Ala Ile Arg Ala Gln Thr Ala Tyr Thr Val Met Ala 
                485                 490                 495     


Ile Thr Gly Met Ala Ile Asp Asn His Leu Leu Ala Leu Arg Glu Leu 
            500                 505                 510         


Ala Arg Ala Met Cys Lys Glu Leu Pro Glu Met Phe Met Asp Glu Thr 
        515                 520                 525             


Tyr Leu Met Ser Asn Arg Phe Val Leu Ser Thr Ser Gln Val Pro Thr 
    530                 535                 540                 


Thr Thr Glu Met Phe Cys Cys Tyr Gly Pro Val Val Pro Asn Gly Tyr 
545                 550                 555                 560 


Gly Ala Cys Tyr Asn Pro Gln Pro Glu Thr Ile Leu Phe Cys Ile Ser 
                565                 570                 575     


Ser Phe His Ser Cys Lys Glu Thr Ser Ser Ser Lys Phe Ala Lys Ala 
            580                 585                 590         


Val Glu Glu Ser Leu Ile Asp Met Arg Asp Leu Cys Ser Leu Leu Pro 
        595                 600                 605             


Pro Thr Glu Ser Lys Pro Leu Ala Thr Lys Glu Lys Ala Thr Arg Pro 
    610                 615                 620                 


Ser Gln Gly His Gln Pro 
625                 630 


