                         SEQUENCE LISTING

<110>  NORWEGIAN UNIVERSITY OF SCIENCE AND TECHNOLOGY (NTNU)
       UNIVERSITT ULM
       NORWEGIAN UNIVERSITY OF LIFE SCIENCES
       DANMARKS TEKNISKE UNIVERSITET
       UNIVERSITT DES SAARLANDES
 
<120>  Production of Bacteriocins

<130>  42.147845/01

<150>  GB2105707.0
<151>  2021-04-21

<160>  79    

<170>  PatentIn version 3.5

<210>  1
<211>  189
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimised pedA gene

<400>  1
atgaagaaga ttgagaagct gaccgaaaag gaaatggcga acatcattgg aggcaagtac       60

tatggcaatg gtgtgacctg tggcaaacac tcctgctctg tcgattgggg caaagccacc      120

acgtgcatca tcaacaacgg tgcaatggca tgggctactg gaggtcatca ggggaaccac      180

aaatgctaa                                                              189


<210>  2
<211>  62
<212>  PRT
<213>  Pediococcus acidilactici

<400>  2

Met Lys Lys Ile Glu Lys Leu Thr Glu Lys Glu Met Ala Asn Ile Ile 
1               5                   10                  15      


Gly Gly Lys Tyr Tyr Gly Asn Gly Val Thr Cys Gly Lys His Ser Cys 
            20                  25                  30          


Ser Val Asp Trp Gly Lys Ala Thr Thr Cys Ile Ile Asn Asn Gly Ala 
        35                  40                  45              


Met Ala Trp Ala Thr Gly Gly His Gln Gly Asn His Lys Cys 
    50                  55                  60          


<210>  3
<211>  44
<212>  PRT
<213>  Pediococcus acidilactici

<400>  3

Lys Tyr Tyr Gly Asn Gly Val Thr Cys Gly Lys His Ser Cys Ser Val 
1               5                   10                  15      


Asp Trp Gly Lys Ala Thr Thr Cys Ile Ile Asn Asn Gly Ala Met Ala 
            20                  25                  30          


Trp Ala Thr Gly Gly His Gln Gly Asn His Lys Cys 
        35                  40                  


<210>  4
<211>  18
<212>  PRT
<213>  Pediococcus acidilactici

<400>  4

Met Lys Lys Ile Glu Lys Leu Thr Glu Lys Glu Met Ala Asn Ile Ile 
1               5                   10                  15      


Gly Gly 
        


<210>  5
<211>  525
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimised pedC gene

<400>  5
atgagcaaga agttctggtc caacatcttt ctggctcttg gtgtgttcct ggcgtttgct       60

ggggttgcaa ccatctcagt ttccgctgat agctctgcca ccattgagtc caacacctcc      120

tcgaagatca tcgatggagc gacgtacgaa gagaacattc gtggtgtcat tcccattacc      180

ctcactcagt atctccacaa agcacaaacg ggcgaaaagt tcatcgtctt tgttggcttc      240

aaggaatgcg tgcattgtcg caaattcagc cctgtgatga agcagtacct tcagcagtct      300

caacacccga tctactacct ggattatggc aacaatggct ccttctctat ggcttcacag      360

aagcaaatca ccgacttcta ctccaccttt gcaaccccaa tgtccttcat gggaactcca      420

acagtagccc tgttggacaa tggcaaagtc gtgtcgatga ctgccggtga tgacaccaca      480

ctgtccgatt tgcagcagat caccgcagac tacaacaacc agtag                      525


<210>  6
<211>  174
<212>  PRT
<213>  Pediococcus acidilactici

<400>  6

Met Ser Lys Lys Phe Trp Ser Asn Ile Phe Leu Ala Leu Gly Val Phe 
1               5                   10                  15      


Leu Ala Phe Ala Gly Val Ala Thr Ile Ser Val Ser Ala Asp Ser Ser 
            20                  25                  30          


Ala Thr Ile Glu Ser Asn Thr Ser Ser Lys Ile Ile Asp Gly Ala Thr 
        35                  40                  45              


Tyr Glu Glu Asn Ile Arg Gly Val Ile Pro Ile Thr Leu Thr Gln Tyr 
    50                  55                  60                  


Leu His Lys Ala Gln Thr Gly Glu Lys Phe Ile Val Phe Val Gly Phe 
65                  70                  75                  80  


Lys Glu Cys Val His Cys Arg Lys Phe Ser Pro Val Met Lys Gln Tyr 
                85                  90                  95      


Leu Gln Gln Ser Gln His Pro Ile Tyr Tyr Leu Asp Tyr Gly Asn Asn 
            100                 105                 110         


Gly Ser Phe Ser Met Ala Ser Gln Lys Gln Ile Thr Asp Phe Tyr Ser 
        115                 120                 125             


Thr Phe Ala Thr Pro Met Ser Phe Met Gly Thr Pro Thr Val Ala Leu 
    130                 135                 140                 


Leu Asp Asn Gly Lys Val Val Ser Met Thr Ala Gly Asp Asp Thr Thr 
145                 150                 155                 160 


Leu Ser Asp Leu Gln Gln Ile Thr Ala Asp Tyr Asn Asn Gln 
                165                 170                 


<210>  7
<211>  2175
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimised pedD gene

<400>  7
atgtggacac agaagtggca caagtactac acagcgcaag tagacgagaa tgattgcggc       60

ctcgcagccc tgaacatgat cttgaagtac tacggttccg attacatgct tgcccatctt      120

cgccagctgg ctaaaaccac agctgacggt actaccgtgc tgggactggt gaaagcagcc      180

aagcacttga acttgaatgc tgaagctgtt cgtgcggaca tggacgcgct gaccgcgtcg      240

caactgcccc tgcctgtgat cgttcacgtt ttcaagaaga acaagctgcc acactactac      300

gtggtctacc aggtgactga gaatgatctc atcattggcg atccggatcc gaccgtgaaa      360

actaccaaga tctccaagtc ccagtttgct aaggagtgga cccagattgc tattatcatc      420

gctccgaccg tcaaatacaa gcccatcaag gaaagccgtc acacgcttat tgaccttgtc      480

cctctcctta tcaaacagaa acgcctgatt ggactgatca ttaccgcagc cgcaatcacc      540

accctgatct ctattgcagg cgcctacttc tttcagctga tcattgacac atatctccca      600

cacctgatga ccaaccgcct ctctctcgtc gcaattgggc ttattgtcgc atacgcattc      660

caggccatca ttaactatat ccagtccttc tttacgatcg tcctgggtca gcggctcatg      720

atcgacattg tgctcaagta tgtccaccac ctcttcgacc tccctatgaa cttctttacc      780

actcggcatg taggtgaaat gacgtcacgc ttttccgatg cttccaaaat cattgacgct      840

ttggggtcaa cgactctgac cctgttcctc gatatgtgga tcctgttggc tgttggactc      900

tttctggcgt atcagaacat caacctgttc ctgtgctcgc tggtagttgt tcccatctat      960

atctccatcg tctggctctt caagaaaacc ttcaaccggc ttaaccagga cactatggaa     1020

tccaatgctg tccttaacag cgcgatcatt gaatctctga gcggtatcga aaccatcaag     1080

tcgcttactg gagaggcgac cacgaagaag aagatcgata ccctgttcag cgatcttctc     1140

cacaagaatc tggcctatca aaaggccgat cagggtcaac aggccatcaa agccgcgaca     1200

aagcttatcc tgacgatcgt gatcctctgg tggggcacct tctttgtcat gcgtcatcag     1260

ctgtccttgg gccagctgtt gacctacaat gcacttcttg cgtacttctt gacgccactc     1320

gagaacatca ttaacctcca accaaaactg caagctgctc gcgttgctaa caatcgcttg     1380

aacgaggttt acctggtaga gtcagagttc tccaaaagcc gagaaatcac tgcacttgaa     1440

cagttgaacg gtgatattga ggtcaaccac gtaagcttca attacggcta ctgctcgaac     1500

attctggaag atgtgtcttt gacgatcccg caccatcaga aaatcaccat tgttggcatg     1560

tctggttccg gcaaaaccac cctggcgaag ttgcttgtgg gcttcttcga accacaagaa     1620

cagcatggcg agattcaaat caaccaccac aacatctccg acatctcccg aaccattctc     1680

cgccagtata tcaactacgt cccacaagaa ccgttcatct tttccggttc cgttctcgaa     1740

aacctgttgc tgggctcccg ccctggcgtg actcaacaga tgatcgacca ggcttgttca     1800

ttcgccgaga ttaagaccga tatcgagaat ctccctcagg gataccatac tcgcctttcc     1860

gagtctggct ttaacctgag cggtggacag aagcagcgtc tttcgatcgc acgtgcactg     1920

ttgtccccag cacagtgttt catctttgac gaatctacct cgaatctgga caccatcacc     1980

gagcacaaaa tcgtgtccaa gctcttgttc atgaaggaca aaaccatcat tttcgtcgca     2040

catcgcttga atatcgcctc tcagactgat aaggtggttg ttctggatca cggcaagatt     2100

gtggagcaag ggtcacaccg tcaactgctg aactacaacg ggtattacgc acgcctcatt     2160

cataaccagg aataa                                                      2175


<210>  8
<211>  724
<212>  PRT
<213>  Pediococcus acidilactici

<400>  8

Met Trp Thr Gln Lys Trp His Lys Tyr Tyr Thr Ala Gln Val Asp Glu 
1               5                   10                  15      


Asn Asp Cys Gly Leu Ala Ala Leu Asn Met Ile Leu Lys Tyr Tyr Gly 
            20                  25                  30          


Ser Asp Tyr Met Leu Ala His Leu Arg Gln Leu Ala Lys Thr Thr Ala 
        35                  40                  45              


Asp Gly Thr Thr Val Leu Gly Leu Val Lys Ala Ala Lys His Leu Asn 
    50                  55                  60                  


Leu Asn Ala Glu Ala Val Arg Ala Asp Met Asp Ala Leu Thr Ala Ser 
65                  70                  75                  80  


Gln Leu Pro Leu Pro Val Ile Val His Val Phe Lys Lys Asn Lys Leu 
                85                  90                  95      


Pro His Tyr Tyr Val Val Tyr Gln Val Thr Glu Asn Asp Leu Ile Ile 
            100                 105                 110         


Gly Asp Pro Asp Pro Thr Val Lys Thr Thr Lys Ile Ser Lys Ser Gln 
        115                 120                 125             


Phe Ala Lys Glu Trp Thr Gln Ile Ala Ile Ile Ile Ala Pro Thr Val 
    130                 135                 140                 


Lys Tyr Lys Pro Ile Lys Glu Ser Arg His Thr Leu Ile Asp Leu Val 
145                 150                 155                 160 


Pro Leu Leu Ile Lys Gln Lys Arg Leu Ile Gly Leu Ile Ile Thr Ala 
                165                 170                 175     


Ala Ala Ile Thr Thr Leu Ile Ser Ile Ala Gly Ala Tyr Phe Phe Gln 
            180                 185                 190         


Leu Ile Ile Asp Thr Tyr Leu Pro His Leu Met Thr Asn Arg Leu Ser 
        195                 200                 205             


Leu Val Ala Ile Gly Leu Ile Val Ala Tyr Ala Phe Gln Ala Ile Ile 
    210                 215                 220                 


Asn Tyr Ile Gln Ser Phe Phe Thr Ile Val Leu Gly Gln Arg Leu Met 
225                 230                 235                 240 


Ile Asp Ile Val Leu Lys Tyr Val His His Leu Phe Asp Leu Pro Met 
                245                 250                 255     


Asn Phe Phe Thr Thr Arg His Val Gly Glu Met Thr Ser Arg Phe Ser 
            260                 265                 270         


Asp Ala Ser Lys Ile Ile Asp Ala Leu Gly Ser Thr Thr Leu Thr Leu 
        275                 280                 285             


Phe Leu Asp Met Trp Ile Leu Leu Ala Val Gly Leu Phe Leu Ala Tyr 
    290                 295                 300                 


Gln Asn Ile Asn Leu Phe Leu Cys Ser Leu Val Val Val Pro Ile Tyr 
305                 310                 315                 320 


Ile Ser Ile Val Trp Leu Phe Lys Lys Thr Phe Asn Arg Leu Asn Gln 
                325                 330                 335     


Asp Thr Met Glu Ser Asn Ala Val Leu Asn Ser Ala Ile Ile Glu Ser 
            340                 345                 350         


Leu Ser Gly Ile Glu Thr Ile Lys Ser Leu Thr Gly Glu Ala Thr Thr 
        355                 360                 365             


Lys Lys Lys Ile Asp Thr Leu Phe Ser Asp Leu Leu His Lys Asn Leu 
    370                 375                 380                 


Ala Tyr Gln Lys Ala Asp Gln Gly Gln Gln Ala Ile Lys Ala Ala Thr 
385                 390                 395                 400 


Lys Leu Ile Leu Thr Ile Val Ile Leu Trp Trp Gly Thr Phe Phe Val 
                405                 410                 415     


Met Arg His Gln Leu Ser Leu Gly Gln Leu Leu Thr Tyr Asn Ala Leu 
            420                 425                 430         


Leu Ala Tyr Phe Leu Thr Pro Leu Glu Asn Ile Ile Asn Leu Gln Pro 
        435                 440                 445             


Lys Leu Gln Ala Ala Arg Val Ala Asn Asn Arg Leu Asn Glu Val Tyr 
    450                 455                 460                 


Leu Val Glu Ser Glu Phe Ser Lys Ser Arg Glu Ile Thr Ala Leu Glu 
465                 470                 475                 480 


Gln Leu Asn Gly Asp Ile Glu Val Asn His Val Ser Phe Asn Tyr Gly 
                485                 490                 495     


Tyr Cys Ser Asn Ile Leu Glu Asp Val Ser Leu Thr Ile Pro His His 
            500                 505                 510         


Gln Lys Ile Thr Ile Val Gly Met Ser Gly Ser Gly Lys Thr Thr Leu 
        515                 520                 525             


Ala Lys Leu Leu Val Gly Phe Phe Glu Pro Gln Glu Gln His Gly Glu 
    530                 535                 540                 


Ile Gln Ile Asn His His Asn Ile Ser Asp Ile Ser Arg Thr Ile Leu 
545                 550                 555                 560 


Arg Gln Tyr Ile Asn Tyr Val Pro Gln Glu Pro Phe Ile Phe Ser Gly 
                565                 570                 575     


Ser Val Leu Glu Asn Leu Leu Leu Gly Ser Arg Pro Gly Val Thr Gln 
            580                 585                 590         


Gln Met Ile Asp Gln Ala Cys Ser Phe Ala Glu Ile Lys Thr Asp Ile 
        595                 600                 605             


Glu Asn Leu Pro Gln Gly Tyr His Thr Arg Leu Ser Glu Ser Gly Phe 
    610                 615                 620                 


Asn Leu Ser Gly Gly Gln Lys Gln Arg Leu Ser Ile Ala Arg Ala Leu 
625                 630                 635                 640 


Leu Ser Pro Ala Gln Cys Phe Ile Phe Asp Glu Ser Thr Ser Asn Leu 
                645                 650                 655     


Asp Thr Ile Thr Glu His Lys Ile Val Ser Lys Leu Leu Phe Met Lys 
            660                 665                 670         


Asp Lys Thr Ile Ile Phe Val Ala His Arg Leu Asn Ile Ala Ser Gln 
        675                 680                 685             


Thr Asp Lys Val Val Val Leu Asp His Gly Lys Ile Val Glu Gln Gly 
    690                 695                 700                 


Ser His Arg Gln Leu Leu Asn Tyr Asn Gly Tyr Tyr Ala Arg Leu Ile 
705                 710                 715                 720 


His Asn Gln Glu 
                


<210>  9
<211>  54
<212>  PRT
<213>  Lactococcus lactis

<400>  9

Met Lys Glu Leu Ser Glu Lys Glu Leu Arg Glu Cys Val Gly Gly Gly 
1               5                   10                  15      


Thr Trp Asp Asp Ile Gly Gln Gly Ile Gly Arg Val Ala Tyr Trp Val 
            20                  25                  30          


Gly Lys Ala Met Gly Asn Met Ser Asp Val Asn Gln Ala Ser Arg Ile 
        35                  40                  45              


Asn Arg Lys Lys Lys His 
    50                  


<210>  10
<211>  39
<212>  PRT
<213>  Lactococcus lactis

<400>  10

Gly Thr Trp Asp Asp Ile Gly Gln Gly Ile Gly Arg Val Ala Tyr Trp 
1               5                   10                  15      


Val Gly Lys Ala Met Gly Asn Met Ser Asp Val Asn Gln Ala Ser Arg 
            20                  25                  30          


Ile Asn Arg Lys Lys Lys His 
        35                  


<210>  11
<211>  60
<212>  PRT
<213>  Lactococcus lactis

<400>  11

Met Lys Asn Asn Asn Asn Phe Phe Lys Gly Met Glu Ile Ile Glu Asp 
1               5                   10                  15      


Gln Glu Leu Val Ser Ile Thr Gly Gly Lys Lys Trp Gly Trp Leu Ala 
            20                  25                  30          


Trp Val Asp Pro Ala Tyr Glu Phe Ile Lys Gly Phe Gly Lys Gly Ala 
        35                  40                  45              


Ile Lys Glu Gly Asn Lys Asp Lys Trp Lys Asn Ile 
    50                  55                  60  


<210>  12
<211>  35
<212>  PRT
<213>  Lactococcus lactis

<400>  12

Lys Lys Trp Gly Trp Leu Ala Trp Val Asp Pro Ala Tyr Glu Phe Ile 
1               5                   10                  15      


Lys Gly Phe Gly Lys Gly Ala Ile Lys Glu Gly Asn Lys Asp Lys Trp 
            20                  25                  30          


Lys Asn Ile 
        35  


<210>  13
<211>  56
<212>  PRT
<213>  Lactobacillus plantarum

<400>  13

Met Leu Gln Phe Glu Lys Leu Gln Tyr Ser Arg Leu Pro Gln Lys Lys 
1               5                   10                  15      


Leu Ala Lys Ile Ser Gly Gly Phe Asn Arg Gly Gly Tyr Asn Phe Gly 
            20                  25                  30          


Lys Ser Val Arg His Val Val Asp Ala Ile Gly Ser Val Ala Gly Ile 
        35                  40                  45              


Arg Gly Ile Leu Lys Ser Ile Arg 
    50                  55      


<210>  14
<211>  33
<212>  PRT
<213>  Lactobacillus plantarum

<400>  14

Phe Asn Arg Gly Gly Tyr Asn Phe Gly Lys Ser Val Arg His Val Val 
1               5                   10                  15      


Asp Ala Ile Gly Ser Val Ala Gly Ile Arg Gly Ile Leu Lys Ser Ile 
            20                  25                  30          


Arg 
    


<210>  15
<211>  52
<212>  PRT
<213>  Lactobacillus plantarum

<400>  15

Met Lys Lys Phe Leu Val Leu Arg Asp Arg Glu Leu Asn Ala Ile Ser 
1               5                   10                  15      


Gly Gly Val Phe His Ala Tyr Ser Ala Arg Gly Val Arg Asn Asn Tyr 
            20                  25                  30          


Lys Ser Ala Val Gly Pro Ala Asp Trp Val Ile Ser Ala Val Arg Gly 
        35                  40                  45              


Phe Ile His Gly 
    50          


<210>  16
<211>  34
<212>  PRT
<213>  Lactobacillus plantarum

<400>  16

Val Phe His Ala Tyr Ser Ala Arg Gly Val Arg Asn Asn Tyr Lys Ser 
1               5                   10                  15      


Ala Val Gly Pro Ala Asp Trp Val Ile Ser Ala Val Arg Gly Phe Ile 
            20                  25                  30          


His Gly 
        


<210>  17
<211>  55
<212>  PRT
<213>  Lactobacillus plantarum

<400>  17

Met Thr Val Asn Lys Met Ile Lys Asp Leu Asp Val Val Asp Ala Phe 
1               5                   10                  15      


Ala Pro Ile Ser Asn Asn Lys Leu Asn Gly Val Val Gly Gly Gly Ala 
            20                  25                  30          


Trp Lys Asn Phe Trp Ser Ser Leu Arg Lys Gly Phe Tyr Asp Gly Glu 
        35                  40                  45              


Ala Gly Arg Ala Ile Arg Arg 
    50                  55  


<210>  18
<211>  25
<212>  PRT
<213>  Lactobacillus plantarum

<400>  18

Gly Ala Trp Lys Asn Phe Trp Ser Ser Leu Arg Lys Gly Phe Tyr Asp 
1               5                   10                  15      


Gly Glu Ala Gly Arg Ala Ile Arg Arg 
            20                  25  


<210>  19
<211>  57
<212>  PRT
<213>  Lactobacillus plantarum

<400>  19

Met Lys Ile Lys Leu Thr Val Leu Asn Glu Phe Glu Glu Leu Thr Ala 
1               5                   10                  15      


Asp Ala Glu Lys Asn Ile Ser Gly Gly Arg Arg Ser Arg Lys Asn Gly 
            20                  25                  30          


Ile Gly Tyr Ala Ile Gly Tyr Ala Phe Gly Ala Val Glu Arg Ala Val 
        35                  40                  45              


Leu Gly Gly Ser Arg Asp Tyr Asn Lys 
    50                  55          


<210>  20
<211>  32
<212>  PRT
<213>  Lactobacillus plantarum

<400>  20

Arg Arg Ser Arg Lys Asn Gly Ile Gly Tyr Ala Ile Gly Tyr Ala Phe 
1               5                   10                  15      


Gly Ala Val Glu Arg Ala Val Leu Gly Gly Ser Arg Asp Tyr Asn Lys 
            20                  25                  30          


<210>  21
<211>  47
<212>  PRT
<213>  Lactobacillus plantarum

<400>  21

Met Asp Lys Phe Glu Lys Ile Ser Thr Ser Asn Leu Glu Lys Ile Ser 
1               5                   10                  15      


Gly Gly Asp Leu Thr Thr Lys Leu Trp Ser Ser Trp Gly Tyr Tyr Leu 
            20                  25                  30          


Gly Lys Lys Ala Arg Trp Asn Leu Lys His Pro Tyr Val Gln Phe 
        35                  40                  45          


<210>  22
<211>  29
<212>  PRT
<213>  Lactobacillus plantarum

<400>  22

Asp Leu Thr Thr Lys Leu Trp Ser Ser Trp Gly Tyr Tyr Leu Gly Lys 
1               5                   10                  15      


Lys Ala Arg Trp Asn Leu Lys His Pro Tyr Val Gln Phe 
            20                  25                  


<210>  23
<211>  55
<212>  PRT
<213>  Lactobacillus plantarum

<400>  23

Met Asn Asn Leu Asn Lys Phe Ser Thr Leu Gly Lys Ser Ser Leu Ser 
1               5                   10                  15      


Gln Ile Glu Gly Gly Ser Val Pro Thr Ser Val Tyr Thr Leu Gly Ile 
            20                  25                  30          


Lys Ile Leu Trp Ser Ala Tyr Lys His Arg Lys Thr Ile Glu Lys Ser 
        35                  40                  45              


Phe Asn Lys Gly Phe Tyr His 
    50                  55  


<210>  24
<211>  34
<212>  PRT
<213>  Lactobacillus plantarum

<400>  24

Ser Val Pro Thr Ser Val Tyr Thr Leu Gly Ile Lys Ile Leu Trp Ser 
1               5                   10                  15      


Ala Tyr Lys His Arg Lys Thr Ile Glu Lys Ser Phe Asn Lys Gly Phe 
            20                  25                  30          


Tyr His 
        


<210>  25
<211>  75
<212>  PRT
<213>  Lactococcus lactis

<400>  25

Met Lys Asn Gln Leu Asn Phe Asn Ile Val Ser Asp Glu Glu Leu Ser 
1               5                   10                  15      


Glu Ala Asn Gly Gly Lys Leu Thr Phe Ile Gln Ser Thr Ala Ala Gly 
            20                  25                  30          


Asp Leu Tyr Tyr Asn Thr Asn Thr His Lys Tyr Val Tyr Gln Gln Thr 
        35                  40                  45              


Gln Asn Ala Phe Gly Ala Ala Ala Asn Thr Ile Val Asn Gly Trp Met 
    50                  55                  60                  


Gly Gly Ala Ala Gly Gly Phe Gly Leu His His 
65                  70                  75  


<210>  26
<211>  54
<212>  PRT
<213>  Lactococcus lactis

<400>  26

Lys Leu Thr Phe Ile Gln Ser Thr Ala Ala Gly Asp Leu Tyr Tyr Asn 
1               5                   10                  15      


Thr Asn Thr His Lys Tyr Val Tyr Gln Gln Thr Gln Asn Ala Phe Gly 
            20                  25                  30          


Ala Ala Ala Asn Thr Ile Val Asn Gly Trp Met Gly Gly Ala Ala Gly 
        35                  40                  45              


Gly Phe Gly Leu His His 
    50                  


<210>  27
<211>  68
<212>  PRT
<213>  Lactococcus lactis

<400>  27

Met Lys Asn Gln Leu Asn Phe Asn Ile Val Ser Asp Glu Glu Leu Ala 
1               5                   10                  15      


Glu Val Asn Gly Gly Ser Leu Gln Tyr Val Met Ser Ala Gly Pro Tyr 
            20                  25                  30          


Thr Trp Tyr Lys Asp Thr Arg Thr Gly Lys Thr Ile Cys Lys Gln Thr 
        35                  40                  45              


Ile Asp Thr Ala Ser Tyr Thr Phe Gly Val Met Ala Glu Gly Trp Gly 
    50                  55                  60                  


Lys Thr Phe His 
65              


<210>  28
<211>  47
<212>  PRT
<213>  Lactococcus lactis

<400>  28

Ser Leu Gln Tyr Val Met Ser Ala Gly Pro Tyr Thr Trp Tyr Lys Asp 
1               5                   10                  15      


Thr Arg Thr Gly Lys Thr Ile Cys Lys Gln Thr Ile Asp Thr Ala Ser 
            20                  25                  30          


Tyr Thr Phe Gly Val Met Ala Glu Gly Trp Gly Lys Thr Phe His 
        35                  40                  45          


<210>  29
<211>  70
<212>  PRT
<213>  Lactococcus garvieae

<400>  29

Met Glu Asn Lys Asn Tyr Thr Val Leu Ser Asp Glu Glu Leu Gln Lys 
1               5                   10                  15      


Ile Asp Gly Gly Glu Tyr His Leu Met Asn Gly Ala Asn Gly Tyr Leu 
            20                  25                  30          


Thr Arg Val Asn Gly Lys Tyr Val Tyr Arg Val Thr Lys Asp Pro Val 
        35                  40                  45              


Ser Ala Val Phe Gly Val Ile Ser Asn Gly Trp Gly Ser Ala Gly Ala 
    50                  55                  60                  


Gly Phe Gly Pro Gln His 
65                  70  


<210>  30
<211>  50
<212>  PRT
<213>  Lactococcus garvieae

<400>  30

Glu Tyr His Leu Met Asn Gly Ala Asn Gly Tyr Leu Thr Arg Val Asn 
1               5                   10                  15      


Gly Lys Tyr Val Tyr Arg Val Thr Lys Asp Pro Val Ser Ala Val Phe 
            20                  25                  30          


Gly Val Ile Ser Asn Gly Trp Gly Ser Ala Gly Ala Gly Phe Gly Pro 
        35                  40                  45              


Gln His 
    50  


<210>  31
<211>  57
<212>  PRT
<213>  Lactococcus lactis

<400>  31

Met Ser Thr Lys Asp Phe Asn Leu Asp Leu Val Ser Val Ser Lys Lys 
1               5                   10                  15      


Asp Ser Gly Ala Ser Pro Arg Ile Thr Ser Ile Ser Leu Cys Thr Pro 
            20                  25                  30          


Gly Cys Lys Thr Gly Ala Leu Met Gly Cys Asn Met Lys Thr Ala Thr 
        35                  40                  45              


Cys Asn Cys Ser Ile His Val Ser Lys 
    50                  55          


<210>  32
<211>  34
<212>  PRT
<213>  Lactococcus lactis

<400>  32

Ile Thr Ser Ile Ser Leu Cys Thr Pro Gly Cys Lys Thr Gly Ala Leu 
1               5                   10                  15      


Met Gly Cys Asn Met Lys Thr Ala Thr Cys Asn Cys Ser Ile His Val 
            20                  25                  30          


Ser Lys 
        


<210>  33
<211>  51
<212>  PRT
<213>  Lactococcus lactis

<400>  33

Met Lys Glu Gln Asn Ser Phe Asn Leu Leu Gln Glu Val Thr Glu Ser 
1               5                   10                  15      


Glu Leu Asp Leu Ile Leu Gly Ala Lys Gly Gly Ser Gly Val Ile His 
            20                  25                  30          


Thr Ile Ser His Glu Cys Asn Met Asn Ser Trp Gln Phe Val Phe Thr 
        35                  40                  45              


Cys Cys Ser 
    50      


<210>  34
<211>  27
<212>  PRT
<213>  Lactococcus lactis

<400>  34

Lys Gly Gly Ser Gly Val Ile His Thr Ile Ser His Glu Cys Asn Met 
1               5                   10                  15      


Asn Ser Trp Gln Phe Val Phe Thr Cys Cys Ser 
            20                  25          


<210>  35
<211>  56
<212>  PRT
<213>  Bacillus subtilis

<400>  35

Met Ser Lys Phe Asp Asp Phe Asp Leu Asp Val Val Lys Val Ser Lys 
1               5                   10                  15      


Gln Asp Ser Lys Ile Thr Pro Gln Trp Lys Ser Glu Ser Leu Cys Thr 
            20                  25                  30          


Pro Gly Cys Val Thr Gly Ala Leu Gln Thr Cys Phe Leu Gln Thr Leu 
        35                  40                  45              


Thr Cys Asn Cys Lys Ile Ser Lys 
    50                  55      


<210>  36
<211>  32
<212>  PRT
<213>  Bacillus subtilis

<400>  36

Trp Lys Ser Glu Ser Leu Cys Thr Pro Gly Cys Val Thr Gly Ala Leu 
1               5                   10                  15      


Gln Thr Cys Phe Leu Gln Thr Leu Thr Cys Asn Cys Lys Ile Ser Lys 
            20                  25                  30          


<210>  37
<211>  56
<212>  PRT
<213>  Staphylococcus epidermidis

<400>  37

Met Glu Asn Lys Lys Asp Leu Phe Asp Leu Glu Ile Lys Lys Asp Asn 
1               5                   10                  15      


Met Glu Asn Asn Asn Glu Leu Glu Ala Gln Ser Leu Gly Pro Ala Ile 
            20                  25                  30          


Lys Ala Thr Arg Gln Val Cys Pro Lys Ala Thr Arg Phe Val Thr Val 
        35                  40                  45              


Ser Cys Lys Lys Ser Asp Cys Gln 
    50                  55      


<210>  38
<211>  30
<212>  PRT
<213>  Staphylococcus epidermidis

<400>  38

Ser Leu Gly Pro Ala Ile Lys Ala Thr Arg Gln Val Cys Pro Lys Ala 
1               5                   10                  15      


Thr Arg Phe Val Thr Val Ser Cys Lys Lys Ser Asp Cys Gln 
            20                  25                  30  


<210>  39
<211>  52
<212>  PRT
<213>  Staphylococcus epidermidis

<400>  39

Met Glu Ala Val Lys Glu Lys Asn Asp Leu Phe Asn Leu Asp Val Lys 
1               5                   10                  15      


Val Asn Ala Lys Glu Ser Asn Asp Ser Gly Ala Glu Pro Arg Ile Ala 
            20                  25                  30          


Ser Lys Phe Ile Cys Thr Pro Gly Cys Ala Lys Thr Gly Ser Phe Asn 
        35                  40                  45              


Ser Tyr Cys Cys 
    50          


<210>  40
<211>  22
<212>  PRT
<213>  Staphylococcus epidermidis

<400>  40

Ile Ala Ser Lys Phe Ile Cys Thr Pro Gly Cys Ala Lys Thr Gly Ser 
1               5                   10                  15      


Phe Asn Ser Tyr Cys Cys 
            20          


<210>  41
<211>  55
<212>  PRT
<213>  Staphylococcus epidermidis

<400>  41

Met Lys Lys Glu Leu Phe Asp Leu Asn Leu Asn Lys Asp Ile Glu Ala 
1               5                   10                  15      


Gln Lys Ser Asp Leu Asn Pro Gln Ser Ala Ser Ile Val Lys Thr Thr 
            20                  25                  30          


Ile Lys Ala Ser Lys Lys Leu Cys Arg Gly Phe Thr Leu Thr Cys Gly 
        35                  40                  45              


Cys His Phe Thr Gly Lys Lys 
    50                  55  


<210>  42
<211>  31
<212>  PRT
<213>  Staphylococcus epidermidis

<400>  42

Ser Ala Ser Ile Val Lys Thr Thr Ile Lys Ala Ser Lys Lys Leu Cys 
1               5                   10                  15      


Arg Gly Phe Thr Leu Thr Cys Gly Cys His Phe Thr Gly Lys Lys 
            20                  25                  30      


<210>  43
<211>  56
<212>  PRT
<213>  Bacillus phage SPBeta

<400>  43

Met Glu Lys Leu Phe Lys Glu Val Lys Leu Glu Glu Leu Glu Asn Gln 
1               5                   10                  15      


Lys Gly Ser Gly Leu Gly Lys Ala Gln Cys Ala Ala Leu Trp Leu Gln 
            20                  25                  30          


Cys Ala Ser Gly Gly Thr Ile Gly Cys Gly Gly Gly Ala Val Ala Cys 
        35                  40                  45              


Gln Asn Tyr Arg Gln Phe Cys Arg 
    50                  55      


<210>  44
<211>  37
<212>  PRT
<213>  Bacillus phage SPBeta

<400>  44

Gly Leu Gly Lys Ala Gln Cys Ala Ala Leu Trp Leu Gln Cys Ala Ser 
1               5                   10                  15      


Gly Gly Thr Ile Gly Cys Gly Gly Gly Ala Val Ala Cys Gln Asn Tyr 
            20                  25                  30          


Arg Gln Phe Cys Arg 
        35          


<210>  45
<211>  66
<212>  PRT
<213>  Carnobacterium piscicola

<400>  45

Met Asn Ser Val Lys Glu Leu Asn Val Lys Glu Met Lys Gln Leu His 
1               5                   10                  15      


Gly Gly Val Asn Tyr Gly Asn Gly Val Ser Cys Ser Lys Thr Lys Cys 
            20                  25                  30          


Ser Val Asn Trp Gly Gln Ala Phe Gln Glu Arg Tyr Thr Ala Gly Ile 
        35                  40                  45              


Asn Ser Phe Val Ser Gly Val Ala Ser Gly Ala Gly Ser Ile Gly Arg 
    50                  55                  60                  


Arg Pro 
65      


<210>  46
<211>  31
<212>  PRT
<213>  Carnobacterium piscicola

<400>  46

Trp Gly Gln Ala Phe Gln Glu Arg Tyr Thr Ala Gly Ile Asn Ser Phe 
1               5                   10                  15      


Val Ser Gly Val Ala Ser Gly Ala Gly Ser Ile Gly Arg Arg Pro 
            20                  25                  30      


<210>  47
<211>  47
<212>  PRT
<213>  Kocuria varians

<400>  47

Met Thr Asn Ala Phe Gln Ala Leu Asp Glu Val Thr Asp Ala Glu Leu 
1               5                   10                  15      


Asp Ala Ile Leu Gly Gly Gly Ser Gly Val Ile Pro Thr Ile Ser His 
            20                  25                  30          


Glu Cys His Met Asn Ser Phe Gln Phe Val Phe Thr Cys Cys Ser 
        35                  40                  45          


<210>  48
<211>  25
<212>  PRT
<213>  Kocuria varians

<400>  48

Gly Ser Gly Val Ile Pro Thr Ile Ser His Glu Cys His Met Asn Ser 
1               5                   10                  15      


Phe Gln Phe Val Phe Thr Cys Cys Ser 
            20                  25  


<210>  49
<211>  64
<212>  PRT
<213>  Streptomyces sp. OH-4156

<400>  49

Met Arg Ser Glu Met Thr Leu Thr Ser Thr Asn Ser Ala Glu Ala Leu 
1               5                   10                  15      


Ala Ala Gln Asp Phe Ala Asn Thr Val Leu Ser Ala Ala Ala Pro Gly 
            20                  25                  30          


Phe His Ala Asp Cys Glu Thr Pro Ala Met Ala Thr Pro Ala Thr Pro 
        35                  40                  45              


Thr Val Ala Gln Phe Val Ile Gln Gly Ser Thr Ile Cys Leu Val Cys 
    50                  55                  60                  


<210>  50
<211>  22
<212>  PRT
<213>  Streptomyces sp. OH-4156

<400>  50

Ala Thr Pro Ala Thr Pro Thr Val Ala Gln Phe Val Ile Gln Gly Ser 
1               5                   10                  15      


Thr Ile Cys Leu Val Cys 
            20          


<210>  51
<211>  52
<212>  PRT
<213>  Staphylococcus gallinarum

<400>  51

Met Glu Ala Val Lys Glu Lys Asn Glu Leu Phe Asp Leu Asp Val Lys 
1               5                   10                  15      


Val Asn Ala Lys Glu Ser Asn Asp Ser Gly Ala Glu Pro Arg Ile Ala 
            20                  25                  30          


Ser Lys Phe Leu Cys Thr Pro Gly Cys Ala Lys Thr Gly Ser Phe Asn 
        35                  40                  45              


Ser Tyr Cys Cys 
    50          


<210>  52
<211>  22
<212>  PRT
<213>  Staphylococcus gallinarum

<400>  52

Ile Ala Ser Lys Phe Leu Cys Thr Pro Gly Cys Ala Lys Thr Gly Ser 
1               5                   10                  15      


Phe Asn Ser Tyr Cys Cys 
            20          


<210>  53
<211>  68
<212>  PRT
<213>  Bacillus sp. strain HIL Y-85,54728

<400>  53

Met Ser Gln Glu Ala Ile Ile Arg Ser Trp Lys Asp Pro Phe Ser Arg 
1               5                   10                  15      


Glu Asn Ser Thr Gln Asn Pro Ala Gly Asn Pro Phe Ser Glu Leu Lys 
            20                  25                  30          


Glu Ala Gln Met Asp Lys Leu Val Gly Ala Gly Asp Met Glu Ala Ala 
        35                  40                  45              


Cys Thr Phe Thr Leu Pro Gly Gly Gly Gly Val Cys Thr Leu Thr Ser 
    50                  55                  60                  


Glu Cys Ile Cys 
65              


<210>  54
<211>  20
<212>  PRT
<213>  Bacillus sp. strain HIL Y-85,54728

<400>  54

Cys Thr Phe Thr Leu Pro Gly Gly Gly Gly Val Cys Thr Leu Thr Ser 
1               5                   10                  15      


Glu Cys Ile Cys 
            20  


<210>  55
<211>  64
<212>  PRT
<213>  Actinoplanes garbadinensis

<400>  55

Met Ser Ala Leu Ala Ile Glu Lys Ser Trp Lys Asp Val Asp Leu Arg 
1               5                   10                  15      


Asp Gly Ala Thr Ser His Pro Ala Gly Leu Gly Phe Gly Glu Leu Thr 
            20                  25                  30          


Phe Glu Asp Leu Arg Glu Asp Arg Thr Ile Tyr Ala Ala Ser Ser Gly 
        35                  40                  45              


Trp Val Cys Thr Leu Thr Ile Glu Cys Gly Thr Val Ile Cys Ala Cys 
    50                  55                  60                  


<210>  56
<211>  19
<212>  PRT
<213>  Actinoplanes garbadinensis

<400>  56

Ser Ser Gly Trp Val Cys Thr Leu Thr Ile Glu Cys Gly Thr Val Ile 
1               5                   10                  15      


Cys Ala Cys 
            


<210>  57
<211>  78
<212>  PRT
<213>  Streptoverticillium griseoverticillatum

<400>  57

Met Thr Ala Ser Ile Leu Gln Gln Ser Val Val Asp Ala Asp Phe Arg 
1               5                   10                  15      


Ala Ala Leu Leu Glu Asn Pro Ala Ala Phe Gly Ala Ser Ala Ala Ala 
            20                  25                  30          


Leu Pro Thr Pro Val Glu Ala Gln Asp Gln Ala Ser Leu Asp Phe Trp 
        35                  40                  45              


Thr Lys Asp Ile Ala Ala Thr Glu Ala Phe Ala Cys Arg Gln Ser Cys 
    50                  55                  60                  


Ser Phe Gly Pro Phe Thr Phe Val Cys Asp Gly Asn Thr Lys 
65                  70                  75              


<210>  58
<211>  19
<212>  PRT
<213>  Streptoverticillium griseoverticillatum

<400>  58

Cys Arg Gln Ser Cys Ser Phe Gly Pro Phe Thr Phe Val Cys Asp Gly 
1               5                   10                  15      


Asn Thr Lys 
            


<210>  59
<211>  77
<212>  PRT
<213>  Streptoverticillium griseoverticillatum

<400>  59

Met Thr Ala Ser Ile Leu Gln Ser Val Val Asp Ala Asp Phe Arg Ala 
1               5                   10                  15      


Ala Leu Ile Glu Asn Pro Ala Ala Phe Gly Ala Ser Thr Ala Val Leu 
            20                  25                  30          


Pro Thr Pro Val Glu Gln Gln Asp Gln Ala Ser Leu Asp Phe Trp Thr 
        35                  40                  45              


Lys Asp Ile Ala Ala Thr Glu Ala Phe Ala Cys Lys Gln Ser Cys Ser 
    50                  55                  60                  


Phe Gly Pro Phe Thr Phe Val Cys Asp Gly Asn Thr Lys 
65                  70                  75          


<210>  60
<211>  19
<212>  PRT
<213>  Streptoverticillium griseoverticillatum

<400>  60

Cys Lys Gln Ser Cys Ser Phe Gly Pro Phe Thr Phe Val Cys Asp Gly 
1               5                   10                  15      


Asn Thr Lys 
            


<210>  61
<211>  19
<212>  PRT
<213>  Streptomyces sp. strain A647P-2

<400>  61

Cys Val Gln Ser Cys Ser Phe Gly Pro Leu Thr Trp Ser Cys Asp Gly 
1               5                   10                  15      


Asn Thr Lys 
            


<210>  62
<211>  44
<212>  PRT
<213>  Enterococcus faecalis

<400>  62

Gly Asp Val Gln Ala Glu Thr Thr Pro Val Cys Ala Val Ala Ala Thr 
1               5                   10                  15      


Ala Ala Ala Ser Ser Ala Ala Cys Gly Trp Val Gly Gly Gly Ile Phe 
            20                  25                  30          


Thr Gly Val Thr Val Val Val Ser Leu Lys His Cys 
        35                  40                  


<210>  63
<211>  68
<212>  PRT
<213>  Enterococcus faecalis

<400>  63

Met Glu Asn Leu Ser Val Val Pro Ser Phe Glu Glu Leu Ser Val Glu 
1               5                   10                  15      


Glu Met Glu Ala Ile Gln Gly Ser Gly Asp Val Gln Ala Glu Thr Thr 
            20                  25                  30          


Pro Val Cys Ala Val Ala Ala Thr Ala Ala Ala Ser Ser Ala Ala Cys 
        35                  40                  45              


Gly Trp Val Gly Gly Gly Ile Phe Thr Gly Val Thr Val Val Val Ser 
    50                  55                  60                  


Leu Lys His Cys 
65              


<210>  64
<211>  27
<212>  PRT
<213>  Enterococcus faecalis

<400>  64

Gly Asp Val Gln Ala Glu Thr Thr Pro Ala Cys Phe Thr Ile Gly Leu 
1               5                   10                  15      


Gly Val Gly Ala Leu Phe Ser Ala Lys Phe Cys 
            20                  25          


<210>  65
<211>  63
<212>  PRT
<213>  Enterococcus faecalis

<400>  65

Met Leu Asn Lys Glu Asn Gln Glu Asn Tyr Tyr Ser Asn Lys Leu Glu 
1               5                   10                  15      


Leu Val Gly Pro Ser Phe Glu Glu Leu Ser Leu Glu Glu Met Glu Ala 
            20                  25                  30          


Ile Gln Gly Ser Gly Asp Val Gln Ala Glu Thr Thr Pro Ala Cys Phe 
        35                  40                  45              


Thr Ile Gly Leu Gly Val Gly Ala Leu Phe Ser Ala Lys Phe Cys 
    50                  55                  60              


<210>  66
<211>  67
<212>  PRT
<213>  Staphylococcus aureus

<400>  66

Met Lys Asn Glu Leu Gly Lys Phe Leu Glu Glu Asn Glu Leu Glu Leu 
1               5                   10                  15      


Gly Lys Phe Ser Glu Ser Asp Met Leu Glu Ile Thr Asp Asp Glu Val 
            20                  25                  30          


Tyr Ala Ala Gly Thr Pro Leu Ala Leu Leu Gly Gly Ala Ala Thr Gly 
        35                  40                  45              


Val Ile Gly Tyr Ile Ser Asn Gln Thr Cys Pro Thr Thr Ala Cys Thr 
    50                  55                  60                  


Arg Ala Cys 
65          


<210>  67
<211>  62
<212>  PRT
<213>  Staphylococcus aureus

<400>  67

Met Lys Ser Ser Phe Leu Glu Lys Asp Ile Glu Glu Gln Val Thr Trp 
1               5                   10                  15      


Phe Glu Glu Val Ser Glu Gln Glu Phe Asp Asp Asp Ile Phe Gly Ala 
            20                  25                  30          


Cys Ser Thr Asn Thr Phe Ser Leu Ser Asp Tyr Trp Gly Asn Lys Gly 
        35                  40                  45              


Asn Trp Cys Thr Ala Thr His Glu Cys Met Ser Trp Cys Lys 
    50                  55                  60          


<210>  68
<211>  63
<212>  PRT
<213>  Streptococcus mutans

<400>  68

Met Ser Asn Thr Gln Leu Leu Glu Val Leu Gly Thr Glu Thr Phe Asp 
1               5                   10                  15      


Val Gln Glu Asp Leu Phe Ala Phe Asp Thr Thr Asp Thr Thr Ile Val 
            20                  25                  30          


Ala Ser Asn Asp Asp Pro Asp Thr Arg Phe Lys Ser Trp Ser Leu Cys 
        35                  40                  45              


Thr Pro Gly Cys Ala Arg Thr Gly Ser Phe Asn Ser Tyr Cys Cys 
    50                  55                  60              


<210>  69
<211>  22
<212>  PRT
<213>  Streptococcus mutans

<400>  69

Phe Lys Ser Trp Ser Leu Cys Thr Pro Gly Cys Ala Arg Thr Gly Ser 
1               5                   10                  15      


Phe Asn Ser Tyr Cys Cys 
            20          


<210>  70
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Class IIA bacteriocin consensus sequence


<220>
<221>  X
<222>  (7)..(7)
<223>  Any amino acid

<400>  70

Tyr Gly Asn Gly Gly Val Xaa Cys 
1               5               


<210>  71
<211>  174
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimised nisZ gene

<400>  71
atgtccacca aagacttcaa cctcgatctt gtgtctgtct ccaagaagga ttctggagct       60

tcacctcgca ttacctccat ctccctgtgt acaccaggct gtaagactgg tgccttgatg      120

ggctgcaaca tgaaaaccgc aacgtgcaat tgcagcatcc acgtttcgaa gtaa            174


<210>  72
<211>  2982
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimised nisB gene

<400>  72
atgatcaaaa gctctttcaa ggctcagccg tttctggtgc gtaacaccat tctgtctccg       60

aacgacaaac gttctttcac cgaatacacc caggtgatcg aaaccgtgag caaaaacaaa      120

gtctttctgg aacagctgct gctggctaac ccgaaactgt ataatgttat gcagaaatac      180

aacgcgggtc tgctgaaaaa gaagcgcgta aagaaactgt tcgagagcat ctataaatac      240

tataagcgca gctacctgcg tagcacccca ttcggtctgt tctccgaaac ctctattggc      300

gtattttcta agtcttccca gtataagctg atgggtaaga ctaccaaggg catccgtctg      360

gacactcagt ggctgattcg cctggtacac aaaatggaag ttgacttctc caaaaaactg      420

tccttcaccc gtaacaacgc caattataaa tttggcgacc gtgtgttcca ggtttatact      480

atcaactcct ccgagctgga agaagtgaac attaaatata ccaacgtata tcagatcatt      540

tccgaatttt gcgaaaatga ctaccagaaa tacgaagaca tttgcgaaac cgttaccctg      600

tgctacggcg acgaataccg tgaactgtct ggccagtatc tgggcagcct gattgtaaac      660

cactacctga tcagcaatct gcaaaaagac ctgctgagcg atttctcctg ggataccttc      720

ctgaccaaag tggaagcgat tgacgaggat aaaaaatata tcatcccgct gaagaaggta      780

cagaaattca ttcaggagta ctccgaaatc gaaatcggtg agggtattga aaaactgaag      840

gaaatttacc aggagatgag ccagattctg gaaaacgaca actacattca aatcgatctg      900

atttccgact ccgagatcaa ctttgatgtt aaacagaaac aacagctgga acacctggcg      960

gagtttctgg gtaacactac caagtccgtg cgccgtactt atctggatga ttacaaggat     1020

aaatttatcg agaaatacgg cgtagaccag gaagttcaaa tcactgaact gtttgacagc     1080

acctttggca tcggtgcgcc gtataactac aaccacccgc gtaacgactt ctacgagtcc     1140

gagccgtcta ctctgtatta ctctgaagaa gaacgtgaaa aatatctgtc tatgtatgtg     1200

gaagcaatta aaaaccacaa cgttattaat ctggatgacc tggaatctca ctaccaaaaa     1260

atggacctgg aaaaaaaatc tgaactgcaa ggcctggagc tgtttctgaa cctggcaaaa     1320

gaatatgaaa aagatatttt catcctgggt gacatcgtag gtaacaataa tctgggtggt     1380

gcatctggtc gtttctctgc actgtccccg gagctgacca gctaccaccg taccatcgtt     1440

gactctgtgg aacgcgagaa cgaaaacaaa gaaatcacca gctgcgaaat cgtattcctg     1500

cctgaaaaca tccgtcatgc caacgttatg cacaccagca tcatgcgtcg taaagtgctg     1560

ccattcttca cgtccacttc ccacaacgaa gttcagctga ctaacattta cattggcatt     1620

gacgaaaaag aaaaattcta tgcgcgtgac attagcaccc aggaagtgct gaagttctac     1680

atcactagca tgtacaacaa aaccctgttt tctaacgaac tgcgttttct gtacgaaatc     1740

agcctggacg ataaattcgg taacctgccg tgggaactga tctatcgtga tttcgactat     1800

atcccgcgcc tggtttttga cgaaatcgta attagcccgg ccaaatggaa aatttggggt     1860

cgtaacgtaa acaacaagat gactatccgt gagctgattc aaagcaaaga gattccgaaa     1920

gagttctata ttgttaacgg tgacaacaaa gtgtatctgt cccaggaaaa cccgctggat     1980

atggaaattc tggaaagcgc catcaaaaaa tctagcaaac gtaaagactt cattgaactg     2040

caagaatact tcgaagacga aaacatcatc aacaagggtc agaaaggtcg tgtggcggac     2100

gtagtggtgc cattcatccg tactcgtgca ctgggtaacg aaggtcgtgc ttttatccgt     2160

gaaaagcgcg tgtctgttga acgccgtgag aaactgccgt ttaacgagtg gctgtatctg     2220

aagctgtaca tctccatcaa ccgtcagaat gagttcctgc tgagctatct gccggacatt     2280

cagaaaatcg tggcaaacct gggtggtaac ctgttttttc tgcgttacac cgacccgaag     2340

cctcacattc gtctgcgcat caaatgcagc gacctgtttc tggcgtacgg ctctattctg     2400

gaaatcctga aacgtagcca gaaaaaccgt attatgtcca ccttcgatat ttccatttat     2460

gatcaggaag tcgagcgtta tggtggtttc gataccctgg aactgtctga agcaatcttc     2520

tgcgcggatt ctaaaatcat cccgaacctg ctgaccctga ttaaagatac caacaacgac     2580

tggaaagtcg atgacgtttc catcctggtt aactacctgt acctgaagtg ctttttccag     2640

aacgataaca agaagattct gaacttcctg aacctggtgt ctccgaaaaa agtaaaggaa     2700

aacgtgaacg aaaaaatcga acactacctg aaactgctga aagtggacaa cctgggcgat     2760

cagattttct acgacaaaaa cttcaaggaa ctgaagcacg caatcaagaa cctgttcctg     2820

aaaatgatcg cgcaggactt tgaactgcaa aaagtatatt ctatcatcga ctccatcatc     2880

cacgttcata acaaccgtct gatcggtatc gagcgtgata aagaaaaact gatctattac     2940

accctgcaac gtctgttcgt tagcgaggaa tatatgaagt ga                        2982


<210>  73
<211>  1803
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimised nisT gene

<400>  73
atggatgaag tgaaggagtt cacatcaaag cagttcttta acacgttgct cacttttccg       60

tcaaccttga agctcatctt ccagctggag aaacgttatg cgatttacct tattgtcctg      120

aacgccatta ccgcatttgt acccctggct tctctgttta tctaccaaga ccttattaac      180

tcagttctgg gctctggtcg gcatcttatc tatatcatca tcatttactt catcgtccag      240

gtgatcacta cggttctggg ccaacttgaa tcctatgtta gcggtaagtt cgacatgcgt      300

ctttcctatt ccatcaatat gcgcttgatg cgcacaacct cctccctgga actttcggat      360

tatgagcagg cagatatgta taacatcatt gagaaggtca ctcaggatag cacctataag      420

ccgttccagc tcttcaatgc gattatcgtg gttctgtcct cgttcatctc actgctctcc      480

tccttgttct tcattggcac ttggaacatc ggagttgcca ttctgctgct gattgtgcct      540

gtgctgtccc tcgtgctgtt tctgcgcgta gggcagctgg agttccttat ccagtggcaa      600

cgcgcttcta gcgaacgtga aacctggtac attgtgtact tgcttaccca tgacttcagc      660

ttcaaggaga tcaagctcaa taacatctcg aactacttta tccacaaatt tggcaagctg      720

aagaaaggct ttatcaatca ggatcttgcc atcgcccgca agaaaaccta cttcaacatc      780

tttctggact tcatcctgaa tttgatcaac atcttgacca tcttcgctat gatcctgtca      840

gtccgagcag gcaaattgtt gattgggaat ctggtttccc tcattcaggc gatttccaag      900

attaacacct actctcagac gatgattcag aacatctaca tcatctacaa cacctccctc      960

ttcatggaac agttgttcga attcctcaaa cgcgaatcag tggttcacaa gaaaatcgag     1020

gatacagaga tctgcaatca ggatatcgga acggtaaaag tcatcaacct gtcctacgtc     1080

taccctaact ccaatgcctt tgcactcaag aacatcaacc tctctttcga gaagggtgaa     1140

ttggcggcta ttgtgggtaa gaacggttct ggcaaatcga ccctcgttaa gatcattagc     1200

ggcctctatc aacccactat gggcattatt cagtacgaca agatgcgttc ctcgctcatg     1260

ccagaggagt tttaccagaa gaacatctcg gtgttgttcc aggacttcgt gaaatacgag     1320

cttaccatcc gcgaaaacat tggtctgagc gacctgtcct cccagtggga agatgagaaa     1380

atcatcaaag tccttgacaa cctcggtctt gacttcctca agaccaacaa ccagtacgtt     1440

ctggacaccc agctggggaa ttggttccaa gaaggacgcc aattgagcgg aggtcaatgg     1500

cagaagatcg cactggcacg gactttcttc aagaaggcgt ctatctacat tctggacgaa     1560

ccatccgctg cactcgatcc agtagcagag aaggagatct tcgactactt tgtcgccctt     1620

tccgaaaaca atatctccat cttcatttct cacagcctta atgctgctcg caaagctaac     1680

aagatcgtcg ttatgaagga tggccaagtc gaagatgtgg gatctcacga tgtcctgctg     1740

cgacgttgtc agtactacca agaactgtac tattccgagc aatacgaaga taacgacgag     1800

taa                                                                   1803


<210>  74
<211>  1245
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimised nisC gene

<400>  74
atgaataaga agaacatcaa acggaacgtt gaaaagatca ttgctcagtg ggatgaacgc       60

acacgcaaga acaaagaaaa cttcgacttt ggcgaactga cgctttccac cggtttgccg      120

ggaattatcc tcatgcttgc cgagttgaag aacaaggata acagcaagat ctaccagaag      180

aagattgaca actatatcga gtacattgtc agcaaactgt ccacctatgg tttgttgacc      240

ggttcgctct actctggagc tgccggaatc gctctgtcta tcctccacct ccgtgaggat      300

gacgagaagt acaagaatct tctggattct ctgaaccgct acatcgagta cttcgttatc      360

gaaaagatcg agggcttcaa tctggagaac atcactccac ccgactacga tgtgatcgaa      420

ggtctgtcag ggattctgtc atacttgctt ctcatcaacg atgaacagta cgacgatctc      480

aagatcctga tcatcaactt tctgtcgaat ctcaccaagg agaacaaggg tttgatctcg      540

ctctacatca aaagcgagaa tcagatgagc caatcagaat cggaaatgta tcctctgggt      600

tgtctgaaca tgggccttgc tcatggcctg gctggagcag ggtgcattct tgcatacgcg      660

cacattaagg ggtattccaa cgaagcatcc ctgtccgcac ttcagaaaat catcttcatc      720

tacgaaaagt tcgaactcga acgcaagaat cagtttctgt ggaaagacgg tctggtagcc      780

gatgaactta agaaagagaa agtgattcga gaagcgtcct tcattcgtga tgcatggtgc      840

tatggtggcc caggcatttc cttgctttac ctctatggag gcctcgcgct tgataacgac      900

tacttcgtgg ataaagccga gaagatcctg gagtctgcga tgcaacgcaa gctggggatc      960

gactcctaca tcatctgtca cggctactca ggcctcattg agatttgctc cttgttcaaa     1020

cggttgctga acacgaagaa gttcgactcc tacattgagg agttcaacgt caattccgag     1080

caaatcctgg aagagtatgg cgatgagtct ggtactggct ttctggaagg catctccgga     1140

tgcattcttg tcttgtccaa gtttgaatac tctatcaact tcacctattg gcgtcaggca     1200

ctgctcctgt tcgacgactt tctgaaaggc ggtaaacgca aataa                     1245


<210>  75
<211>  213
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimised garQ gene

<400>  75
atggagaaca acaactacac cgtgttgtcc gatgaagagc ttcagaagat cgatggaggc       60

gaataccacc tgatgaatgg tgccaatggc tatctcactc gtgtcaacgg caagtacgtc      120

tatcgcgtta ccaaagaccc agtttccgcg gtattcggcg tgattagcaa cggttgggga      180

tctgctggtg cagggtttgg ccctcaacat taa                                   213


<210>  76
<211>  314
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimised garI gene

<400>  76
tgtttacctc caagaagatc aaacgcgatg aacaggtgtt cctgaacctt ctgtacgact       60

tcgtgctttc tgagaacatc actgatcgtg aacggaaaat cggcttgttg gccaagaagg      120

acgtagagaa cggcaaatac ctccttgcag tcctgaacaa ggttagctca tccatgcaaa      180

aggaagcgat gaagaatggt ctgtccattg atgcatccag cttctacaag aaactgggtc      240

caatcatcac ctccattgct cctattgggc tgaatcgcgg atctatgctc gtcaacaact      300

cgtatctcga ctaa                                                        314


<210>  77
<211>  2148
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimised garC gene

<400>  77
atgcggttca agaagaagta ctatacctcc caagtggatg agcgcgactg tggttgcgca       60

gcactgtcca tggtgctcaa aacgtatgga accgaacagt cactggcatc tatccgtctg      120

ttggctggta ctacaattga gggtacttcc gctctgggaa tcaagaaggc tgctgaacac      180

ctgaagttct ccgtgcaagc ctttcgcgca gacccaacac tcttcgatac caagaagttg      240

ccttacccgt tcatcgtcca tgtcgtcaaa gagaagaaat accctcacta ctacgttgtc      300

aagtcgtcga acaagaaaca cattatcatc gcagatccag accctaccgt caagatccgt      360

aaaatgacct gggaccagtt ttactccgag tggacaggcg taaccttgtt catcgcacca      420

cagccatcct acaaacccgt gaaggagaag tccgaatctt tgttctcgtt catcccaatc      480

cttgctaagc agaagctgct ggtgttccag atcattattg cgtcccttct ggttacactg      540

attaacatca ttggcagcta ctacctccaa aacatcatcg acgagtatgt ccccaatgcc      600

ctgatgaaaa ctctgagcat cgtgtcgctc ggtctgatgg ctacgtactt gattcagcag      660

ttcctcaact ttgcgcagac ctttctcctg actatcctgg ggcaacgttt ggctatcgac      720

gttatccttt catacatccg gcacatcttt cagctcccga tgtccttctt ttcgacccga      780

cgtacgggtg aaatcactag ccgcttttca gatgcgaact ccatcatcga tgcgttggct      840

agcgctattc tgtccctctt tctcgacgta accatcgtta ttctgactgg cttggttctg      900

ggtttgcaga acatgtctct cttcttcctg gtgcttttca gcattccgct ctatgtcctt      960

atcatcttta tcttcattcc gttgttcgag aagcagaaca acgaagtcat gcaggcgaat     1020

tccattctga attcctcaat catcgaggac atcaacggta tcgaaaccat caaatccctg     1080

ggctctgaag agattcgcta tcagaaaatt gatcgcgaat tcgccgatta catgaagaaa     1140

tcgttcacgc gccagaagtc ggaggcattc cagactgccc tgaagtccgg tctgcaactg     1200

gttctgaacg tgttgattct ttggtacggt gccactctgg tgatgtccca gaaaatcacc     1260

ctcggacaac ttatcacctt caatgctctt ctgtcctact tcatgacccc actttctaac     1320

atcatcaatc tccagaccaa gctccaatct gccaaggtcg cgaaccatcg gctgaatgaa     1380

gtttacctgg tggatagcga gtttgacacg aacaccgatg aggttatcct ttccaacttt     1440

gagatgcagc tgatcggcat tagctatcac tacggctttg ggcgtgacat tctttctaac     1500

atgtccctta acatcaaaga aaacgaaaag cttaccattg tcggaatgtc tggctcaggc     1560

aaatccaccc tcgttaagct tctcgtaaac ttcttcgaac ccacccaagg aaccattatc     1620

ctgggcggga ttgacatcaa acaaatggat aaacaccagc tgcgttctat cgtgtcatat     1680

ttgcctcaac agccttacat cttcaccggc accgtcttgg agaaccttat tctgggcgcg     1740

tctgaagatc tctctcagga agagattttc caggcagtag aggcagcaga aatccgatcc     1800

gatatcgaac agctccaact gggctatcag acggagctgt catccgatgc cacaactctc     1860

tcgggtggac agaagcaacg cattgcgctc gctcgcgcac ttctgtctcc agccaagatc     1920

ctgatcttcg atgaggctac cagcaatctg gacgttatta ccgagaagaa gattttgaag     1980

aatctcttca aactggacaa gaccatcatc ttcatcgccc atcgcttgag cattgcagaa     2040

aagtccgacc gcatcattgt gatcgatcag gggaaaatca tggaagaagg ctcccacttg     2100

gaactgcttt ccaagaatgg cttctacgcc cagatctaca accagtaa                  2148


<210>  78
<211>  1428
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimised garD gene

<400>  78
gtgtttgata agcgactctt ggagtcgtct gagctgtatg aaaagcgcta caagaatttc       60

tccgtcctcc tcatctttcc agtcgcactg ttgtttatcg gtctgttcgt gttcagcttc      120

ttcgctaaga aggagctgat cgtgacgaac attgcctcca ttgctccgaa gaagatcatc      180

agcaacattc aatccacctc caatacgccc attatcgaaa accacctggt tgaggggaaa      240

tcagtgcagt ctaattcact gctcatcaag tacaacaacg actctgactc tactcagatt      300

accaccctga tggatcaaaa gcaggaattc ctcaacaaga aggagcaact tcagttgctt      360

ctgaattcgc tgcactctga taccaaccag ttcccaaaca ccgattccta tggatacgaa      420

aagacgttcg aaacctacaa ggcacaagcc gaatcccttc gcgaatcgat ccagaaatcg      480

aaccaagtgg ttgacgatca gaacaactcc attgagaacc agaagtctgc aatcaccacg      540

cagatttcca acgtaaacac ccaaatcaat gcttacatcg acatccagaa tgcagctagc      600

accaacagct cagtatcctc caacaatccg tacatcgctc agtataactc gtacttggaa      660

cagcgcaagg ttctcgaaga atccatgaag aatcaggaaa agactgaagc ctcctcaaag      720

gccgacatta cccagcagaa agaggccttg aaagcgcaat tcatctccaa catctcctcc      780

aacattgaca accttaagaa tcagatccag acactggagg tccagaaatc ctccctgagc      840

agctccaact cttacgatca atctcagaaa agccaactcc tcgcgcttaa aactcaggcg      900

ctcactgctg cgaacaaaga actgtccgac atcaatagca cacttaccga gattggtggc      960

aaaatctctc tccagaaaca ggccaaccaa tacaacgcaa tctttgctga gaaagagggt     1020

atcttgcatg ttctgcctaa cgtgctggag aacaaaacct tccaggtagg tactccactg     1080

gcacagatct atcctgctct gaaggcgaag tcacaggtct atctgacttc atacattccc     1140

tccacccaga tttcaggcat taagcttgga cagcgtgcac gcttcacggt gcagcaaaac     1200

ttgcctaagc ttgagatcct gactggaacc atcaagcaga tcgattccgc accaacagcc     1260

cttaaggagg gtaattcgta caaagtgtcg gcactggtta ccttgaacga acgtgatctc     1320

tcctacatcc ggtatggctt ggaaggcaag tttgtcgtcg ttaccggcca aaagacctac     1380

ttcaactact acctggacaa aatcaagggc caagattctc tgaattaa                  1428


<210>  79
<211>  31
<212>  PRT
<213>  Bacillus subtilis

<400>  79

Met Lys Lys Leu Leu Thr Val Met Thr Met Ala Val Leu Thr Ala Gly 
1               5                   10                  15      


Thr Leu Leu Leu Pro Ala Gln Ser Val Thr Pro Ala Ala His Ala 
            20                  25                  30      


