                         SEQUENCE LISTING

<110>  Versameb AG
 
<120>  Method of treating lower urinary tract symptoms

<130>  P6222PC00

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pro-domain of human IGF1

<400>  1
gtgaagatgc acaccatgag cagcagccac ctgttctatc tggccctgtg cctgctgacc       60

tttaccagct ctgctaccgc c                                                 81


<210>  2
<211>  210
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mature human IGF1

<400>  2
ggacctgaga cactttgtgg cgctgaactg gtggacgccc tgcagtttgt gtgtggcgac       60

agaggcttct acttcaacaa gcccacaggc tacggcagca gctctagaag ggctcctcag      120

accggaatcg tggacgagtg ctgcttcaga agctgcgacc tgcggcggct ggaaatgtat      180

tgtgcccctc tgaagcctgc caagagcgcc                                       210


<210>  3
<211>  18
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Thr Ile Leu Phe Leu Thr Met Val Ile Ser Tyr Phe Gly Cys Met 
1               5                   10                  15      


Lys Ala 
        


<210>  4
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  signal peptide of human BDNF

<400>  4
atgaccatcc tgtttctgac aatggtcatc agctacttcg gctgcatgaa ggcc             54


<210>  5
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  signal peptide of human Insulin growth factor 1 (IGF1)

<400>  5
atgggcaaga ttagcagcct gcctacacag ctgttcaagt gctgcttctg cgacttcctg       60

aaa                                                                     63


<210>  6
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  6

Met Gly Lys Ile Ser Ser Leu Pro Thr Gln Leu Phe Lys Cys Cys Phe 
1               5                   10                  15      


Cys Asp Phe Leu Lys 
            20      


<210>  7
<211>  348
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of Cpd.1A and Cpd.1B

<400>  7
atgaccatcc tgtttctgac aatggtcatc agctacttcg gctgcatgaa ggccgtgaag       60

atgcacacca tgagcagcag ccacctgttc tatctggccc tgtgcctgct gacctttacc      120

agctctgcta ccgccggacc tgagacactt tgtggcgctg aactggtgga cgccctgcag      180

tttgtgtgtg gcgacagagg cttctacttc aacaagccca caggctacgg cagcagctct      240

agaagggctc ctcagaccgg aatcgtggac gagtgctgct tcagaagctg cgacctgcgg      300

cggctggaaa tgtattgtgc ccctctgaag cctgccaaga gcgcctaa                   348


<210>  8
<211>  348
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence of Cpd.1A and Cpd.1B


<220>
<221>  misc_feature
<222>  (1)..(348)
<223>  m1f (uridine is 1-methylpseudouridine)

<400>  8
augaccaucc uguuucugac aauggucauc agcuacuucg gcugcaugaa ggccgugaag       60

augcacacca ugagcagcag ccaccuguuc uaucuggccc ugugccugcu gaccuuuacc      120

agcucugcua ccgccggacc ugagacacuu uguggcgcug aacuggugga cgcccugcag      180

uuugugugug gcgacagagg cuucuacuuc aacaagccca caggcuacgg cagcagcucu      240

agaagggcuc cucagaccgg aaucguggac gagugcugcu ucagaagcug cgaccugcgg      300

cggcuggaaa uguauugugc cccucugaag ccugccaaga gcgccuaa                   348


<210>  9
<211>  2740
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of vector pMA-T with Cpd.1A

<400>  9
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc       60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga      120

gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt      180

gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt      240

gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg      300

acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca      360

aggccacgtg tcttgtccag agctcggatc cgccaccatg accatcctgt ttctgacaat      420

ggtcatcagc tacttcggct gcatgaaggc cgtgaagatg cacaccatga gcagcagcca      480

cctgttctat ctggccctgt gcctgctgac ctttaccagc tctgctaccg ccggacctga      540

gacactttgt ggcgctgaac tggtggacgc cctgcagttt gtgtgtggcg acagaggctt      600

ctacttcaac aagcccacag gctacggcag cagctctaga agggctcctc agaccggaat      660

cgtggacgag tgctgcttca gaagctgcga cctgcggcgg ctggaaatgt attgtgcccc      720

tctgaagcct gccaagagcg cctaagaatt cggtacctgg agcacaagac tggcctcatg      780

ggccttccgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaaca      840

tggtcatagc tgtttccttg cgtattgggc gctctccgct tcctcgctca ctgactcgct      900

gcgctcggtc gttcgggtaa agcctggggt gcctaatgag caaaaggcca gcaaaaggcc      960

aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag     1020

catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac     1080

caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc     1140

ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt     1200

aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc     1260

gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga     1320

cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta     1380

ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta     1440

tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga     1500

tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg     1560

cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag     1620

tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc     1680

tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact     1740

tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt     1800

cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta     1860

ccatctggcc ccagtgctgc aatgataccg cgagaaccac gctcaccggc tccagattta     1920

tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc     1980

gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat     2040

agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt     2100

atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg     2160

tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca     2220

gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta     2280

agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg     2340

cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact     2400

ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg     2460

ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt     2520

actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga     2580

ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc     2640

atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa     2700

caaatagggg ttccgcgcac atttccccga aaagtgccac                           2740


<210>  10
<211>  2894
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of vector pMK with Cpd.1B

<400>  10
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc       60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga      120

gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt      180

gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt      240

gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg      300

acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca      360

aggccgcatg tcttgtccag agctcggatc cgccaccatg accatcctgt ttctgacaat      420

ggtcatcagc tacttcggct gcatgaaggc cgtgaagatg cacaccatga gcagcagcca      480

cctgttctat ctggccctgt gcctgctgac ctttaccagc tctgctaccg ccggacctga      540

gacactttgt ggcgctgaac tggtggacgc cctgcagttt gtgtgtggcg acagaggctt      600

ctacttcaac aagcccacag gctacggcag cagctctaga agggctcctc agaccggaat      660

cgtggacgag tgctgcttca gaagctgcga cctgcggcgg ctggaaatgt attgtgcccc      720

tctgaagcct gccaagagcg cctaagaatt cggtacctgg agcacaagac tggcctcatg      780

ggccttccgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaaca      840

tggtcatagc tgaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      900

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      960

aaaaaaaaaa aactcgaggc tcttcctggg cctcatgggc cttcctttca ctgcccgctt     1020

tccagtcggg aaacctgtcg tgccagctgc attaacatgg tcatagctgt ttccttgcgt     1080

attgggcgct ctccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cgggtaaagc     1140

ctggggtgcc taatgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt     1200

gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag     1260

tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc     1320

cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc     1380

ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt     1440

cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt     1500

atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc     1560

agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa     1620

gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa     1680

gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg     1740

tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga     1800

agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg     1860

gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg     1920

aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt attagaaaaa     1980

ttcatccagc agacgataaa acgcaatacg ctggctatcc ggtgccgcaa tgccatacag     2040

caccagaaaa cgatccgccc attcgccgcc cagttcttcc gcaatatcac gggtggccag     2100

cgcaatatcc tgataacgat ccgccacgcc cagacggccg caatcaataa agccgctaaa     2160

acggccattt tccaccataa tgttcggcag gcacgcatca ccatgggtca ccaccagatc     2220

ttcgccatcc ggcatgctcg ctttcagacg cgcaaacagc tctgccggtg ccaggccctg     2280

atgttcttca tccagatcat cctgatccac caggcccgct tccatacggg tacgcgcacg     2340

ttcaatacga tgtttcgcct gatgatcaaa cggacaggtc gccgggtcca gggtatgcag     2400

acgacgcatg gcatccgcca taatgctcac tttttctgcc ggcgccagat ggctagacag     2460

cagatcctga cccggcactt cgcccagcag cagccaatca cggcccgctt cggtcaccac     2520

atccagcacc gccgcacacg gaacaccggt ggtggccagc cagctcagac gcgccgcttc     2580

atcctgcagc tcgttcagcg caccgctcag atcggttttc acaaacagca ccggacgacc     2640

ctgcgcgctc agacgaaaca ccgccgcatc agagcagcca atggtctgct gcgcccaatc     2700

atagccaaac agacgttcca cccacgctgc cgggctaccc gcatgcaggc catcctgttc     2760

aatcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag     2820

cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc     2880

ccgaaaagtg ccac                                                       2894


<210>  11
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of T7 Promoter

<400>  11
taatacgact cactata                                                      17


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of Forward Primer for IVT template generation

<400>  12
gctgcaaggc gattaagttg                                                   20


<210>  13
<211>  143
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of Reverse Primer for IVT template generation


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  T is 2'-O-Methyluridine

<400>  13
tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt       60

tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt      120

cagctatgac catgttaatg cag                                              143


<210>  14
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of Forward qPCR Primer

<400>  14
cggtctgagg agcccttcta g                                                 21


<210>  15
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of Reverse qPCR Primer

<400>  15
cgacagaggc ttctacttca acaag                                             25


<210>  16
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of qPCR Probe


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Fluoescent label at 5'

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  MGB-Q500 (Quencher) at 3'

<400>  16
ctgctgccgt agcctg                                                       16


