               SEQUENCE LISTING

<110> Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University

<120> PROCESS FOR THE DIRECT ENZYMATIC CONVERSION OF AMINO SUGARS; ENZYME AND COMPOSITIONS FOR USE IN THE PROCESS.

<130> K 70 707

<160> 36

<170> BiSSAP 1.3.6

<210> 1
<211> 252
<212> PRT
<213> Enterococcus faecalis


<400> 1
Met Lys Trp Met Ile Val Glu Lys Ala Glu Glu Leu Ala Asn Val Ser 
1               5                   10                  15      
Tyr Gln Leu Leu Lys Gln Glu Ile Val Arg His Pro Glu Gly Leu Thr 
            20                  25                  30          
Ile Gly Leu Ala Thr Gly Ser Ser Pro Leu Gly Val Tyr Glu Glu Trp 
        35                  40                  45              
Arg Lys Asp Arg Val Asp Cys Arg His Val Thr Thr Val Asn Leu Asp 
    50                  55                  60                  
Glu Tyr Val Gly Leu Ser Pro Asp His Pro His Ser Tyr His Thr Phe 
65                  70                  75                  80  
Met Gln Glu His Leu Phe Asp Ala Val Asp Phe Lys Glu Ser Tyr Val 
                85                  90                  95      
Pro Ile Gly Ser Thr Ala Asp Pro Arg Glu Glu Ser Asp Arg Tyr Glu 
            100                 105                 110         
Ala Leu Val Arg Gln Leu Gly Ile Asp Ile Gln Leu Leu Gly Ile Gly 
        115                 120                 125             
Ser Asn Gly His Ile Ala Phe Asn Glu Pro Gly Thr Pro Phe Asp Ala 
    130                 135                 140                 
Lys Thr His Val Thr Lys Leu Thr Glu Ser Thr Arg Gln Ala Asn Gln 
145                 150                 155                 160 
Arg Phe Phe Asp Arg Leu Glu Asp Val Pro Thr Glu Ala Ile Thr Met 
                165                 170                 175     
Gly Ile Gly Thr Ile Met Glu Ala Lys Lys Ile Leu Leu Val Ala Ser 
            180                 185                 190         
Ser Glu Arg Lys Ala Glu Ala Ile Arg Asp Met Met Glu Gly Pro Ala 
        195                 200                 205             
Thr Thr Asp Cys Pro Ala Thr Ile Leu Gln Arg His Ala Asp Val Met 
    210                 215                 220                 
Val Val Leu Asp Glu Glu Ala Ala Ser Leu Leu Ser Asp Glu Ala Lys 
225                 230                 235                 240 
Arg Thr Gly Arg Ala Ala Tyr Thr Asn Phe Met Lys 
                245                 250         

<210> 2
<211> 702
<212> DNA
<213> Enterococcus faecalis


<400> 2
atgcagatca tccgtgttgc taacgctgaa gaaggtggta aaaaagcttt cgaactgatc      60

aaagaaggta tgaacaacgg tgctaaagtt ctgggtctgg ctaccggttc taccccggaa     120

accctgtaca aagaaatgac cgcttctgac atcgacttca ccgaaatgac ctctgttaac     180

ctggacgaat acgttggtct gggtggtgaa gacgaacagt cttaccgtta cttcatgaac     240

aaacacctgt tcgacaaaaa accgttcaaa gaaaccttcg ttccgaacgg taaagctgaa     300

gacctggacg ctgcttctgc tgaatacgaa aaaatcatcg acgctcaccc ggttgacatc     360

cagatcctgg gtatcggtca gaacggccat atcggcttca acgaaccggg caccccgctg     420

gactctctga cccacgttgt tgaactgacc gaatctacca tcaacgctaa caaacgttac     480

ttcgacaaag ttgaagacgt tccgacccgt gctgtttcta tgggtatcgg ttctatcatg     540

aaaggtaaaa aaatgatcct gatggcttac ggtgaagcta aagctgaagc tatcaaaggt     600

atgatcgacg gtccggttac caccgacatg ccggcttctg ctctgcagaa ccaccaggac     660

gttgttgtta tcatcgacga cgctgctgct tctaaactgt aa                        702


<210> 3
<211> 252
<212> PRT
<213> Exiguobacterium sibiricum 
<223> Es1 Variant

<400> 3
Met Lys Trp Met Ile Val Glu Lys Ala Glu Glu Leu Ala Asn Val Ser 
1               5                   10                  15      
Tyr Gln Leu Leu Lys Gln Glu Ile Val Arg His Pro Glu Gly Leu Thr 
            20                  25                  30          
Ile Gly Leu Ala Thr Gly Ser Ser Pro Leu Gly Val Tyr Glu Glu Trp 
        35                  40                  45              
Arg Lys Asp Arg Val Asp Cys Arg His Val Thr Thr Val Asn Leu Asp 
    50                  55                  60                  
Glu Tyr Val Gly Leu Ser Pro Asp His Pro His Ser Tyr His Thr Phe 
65                  70                  75                  80  
Met Gln Glu His Leu Phe Asp Ala Val Asp Phe Lys Glu Ser Tyr Val 
                85                  90                  95      
Pro Ile Gly Ser Thr Ala Asp Pro Arg Glu Glu Ser Asp Arg Tyr Glu 
            100                 105                 110         
Ala Leu Val Arg Gln Leu Gly Ile Asp Ile Gln Leu Leu Gly Ile Gly 
        115                 120                 125             
Ser Asn Gly His Ile Ala Phe Asn Glu Pro Gly Thr Pro Phe Asp Ala 
    130                 135                 140                 
Lys Thr His Val Thr Lys Leu Thr Glu Ser Thr Arg Gln Ala Asn Gln 
145                 150                 155                 160 
Arg Phe Phe Asp Arg Leu Glu Asp Val Pro Thr Glu Ala Ile Thr Met 
                165                 170                 175     
Gly Ile Gly Thr Ile Met Glu Ala Lys Lys Ile Leu Leu Val Ala Ser 
            180                 185                 190         
Ser Glu Arg Lys Ala Glu Ala Ile Arg Asp Met Met Glu Gly Pro Ala 
        195                 200                 205             
Thr Thr Asp Cys Pro Ala Thr Ile Leu Gln Arg His Ala Asp Val Met 
    210                 215                 220                 
Val Val Leu Asp Glu Glu Ala Ala Ser Leu Leu Ser Asp Glu Ala Lys 
225                 230                 235                 240 
Arg Thr Gly Arg Ala Ala Tyr Thr Asn Phe Met Lys 
                245                 250         

<210> 4
<211> 759
<212> DNA
<213> Exiguobacterium sibiricum 
<223> Es1 Variant

<400> 4
atgaaatgga tgatcgttga aaaagctgaa gaactggcta acgtttctta ccagctgctg      60

aaacaggaaa tcgttcgtca cccggaaggt ctgaccatcg gtctggctac cggttcttct     120

ccgctgggtg tttacgaaga atggcgtaaa gaccgtgttg actgccgtca cgttaccacc     180

gttaacctgg acgaatacgt tggtctgtct ccggaccacc cgcactctta ccacaccttc     240

atgcaggaac acctgttcga cgctgttgac ttcaaagaat cttacgttcc gatcggttct     300

accgctgacc cgcgtgaaga atctgaccgt tacgaagctc tggttcgtca gctgggtatc     360

gacatccagc tgctgggtat cggttctaac ggccatatcg cgttcaacga accgggcacc     420

ccgttcgacg ctaaaaccca cgttaccaaa ctgaccgaat ctacccgtca ggctaaccag     480

cgtttcttcg accgtctgga agacgttccg accgaagcta tcactatggg tatcggtacc     540

atcatggaag ctaaaaaaat cctgctggtt gcttcttctg aacgtaaagc tgaagctatc     600

cgtgacatga tggaaggtcc ggctaccacc gactgcccgg ctaccatcct gcagcgtcac     660

gctgacgtta tggttgttct ggacgaagaa gctgcttctc tgctgtctga cgaagctaaa     720

cgtaccggtc gtgctgctta caccaacttc atgaaataa                            759


<210> 5
<211> 598
<212> PRT
<213> Exiguobacterium sibiricum 
<223> Es2 Variant

<400> 5
Met Cys Gly Ile Val Gly Met Ile Gly Gln Val Asn Thr Lys Glu Ile 
1               5                   10                  15      
Leu Leu Lys Gly Leu Glu Lys Leu Glu Tyr Arg Gly Tyr Asp Ser Ala 
            20                  25                  30          
Gly Leu Ala Phe Val Asn Asp Gly Val Gln Val His Lys Glu Val Gly 
        35                  40                  45              
Arg Ile Ala Ala Leu Arg Glu Val Val Pro Ala Glu Ala Asp Gly Leu 
    50                  55                  60                  
Val Gly Ile Gly His Thr Arg Trp Ala Thr His Gly Val Pro Ser Val 
65                  70                  75                  80  
Pro Asn Ala His Pro His Gln Ser Ala Ser Ser Arg Phe Thr Leu Val 
                85                  90                  95      
His Asn Gly Val Ile Glu Asn Asp Glu Gln Leu Lys Ala Glu Leu Asn 
            100                 105                 110         
Val Asp Leu Leu Ser Asp Thr Asp Thr Glu Val Ile Val Gln Met Ile 
        115                 120                 125             
Glu Lys Asn Phe Ala Glu Thr Asn Asp Val Val Glu Ala Phe Arg Gln 
    130                 135                 140                 
Thr Leu Arg Val Leu His Gly Ser Tyr Ala Leu Ala Leu Ile Asp Ala 
145                 150                 155                 160 
Glu Asn Pro Asp Val Leu Tyr Val Ala Lys Asn Lys Ser Pro Leu Leu 
                165                 170                 175     
Val Gly Leu Gly Asp Gly Thr Phe Asn Val Val Ala Ser Asp Ala Met 
            180                 185                 190         
Ala Met Leu Gln Val Thr Asp Gln Phe Val Glu Leu His Asp Gly Glu 
        195                 200                 205             
Met Ile Ile Leu Thr Arg Asp Ser Val Thr Ile Gln Asp Leu Asp Gly 
    210                 215                 220                 
Asn Val Lys Glu Arg Glu Ala Tyr Thr Ala Glu Ile Asp Ala Ser Asp 
225                 230                 235                 240 
Ile Glu Lys Gly Thr Tyr Ala His Tyr Met Leu Lys Glu Met Asp Glu 
                245                 250                 255     
Gln Pro Ala Val Ile Arg Asn Ile Val Gln Lys Tyr Gln Asn Glu Ser 
            260                 265                 270         
Gly Glu Ile Thr Leu Asp Gln Ser Val Arg Asp Leu Val Leu Gly Arg 
        275                 280                 285             
Asp Arg Val Tyr Ile Ile Gly Cys Gly Thr Ser Tyr His Ala Gly Leu 
    290                 295                 300                 
Ile Gly Lys Gln Leu Ile Glu Gln Ile Ala Gly Ile Pro Thr Glu Val 
305                 310                 315                 320 
His Ile Ser Ser Glu Phe Gly Tyr Asn Met Pro Leu Leu Thr Glu Lys 
                325                 330                 335     
Pro Leu Phe Leu Phe Leu Ser Gln Ser Gly Glu Thr Ala Asp Ser Arg 
            340                 345                 350         
Ala Val Leu Val Glu Ala Lys Lys Leu Gly His Pro Ala Leu Thr Ile 
        355                 360                 365             
Thr Asn Val Ala Gly Ser Thr Leu Ser Arg Glu Ala Asn Ala Thr Leu 
    370                 375                 380                 
Leu Leu His Ala Gly Pro Glu Ile Ala Val Ala Ser Thr Lys Ala Tyr 
385                 390                 395                 400 
Thr Ala Gln Ile Ala Val Leu Ala Val Leu Ala Phe Asp Leu Ala Gln 
                405                 410                 415     
Ala Lys Gly Val Asp Val Asn Phe Asp Leu Met Lys Glu Leu Gly Lys 
            420                 425                 430         
Ile Ser Ser Ala Met Glu Ser Val Met Ser Gln Lys Glu Arg Phe Gln 
        435                 440                 445             
Glu Ile Ala Ser Glu Tyr Leu Ser Glu Ser Arg Asn Ala Phe Phe Ile 
    450                 455                 460                 
Gly Arg Gly Gln Asp Ala Tyr Val Gly Met Glu Gly Ala Leu Lys Leu 
465                 470                 475                 480 
Lys Glu Ile Ser Tyr Ile Gln Ala Glu Gly Tyr Ala Gly Gly Glu Leu 
                485                 490                 495     
Lys His Gly Pro Ile Ala Leu Ile Glu Asp Asn Thr Pro Val Ile Ala 
            500                 505                 510         
Leu Val Thr Gln Pro His Val His Leu Asn Asn Arg Gly Asn Val Lys 
        515                 520                 525             
Glu Val Val Ala Arg Gly Ala Asn Ala Cys Val Ile Ala Ala Glu Gly 
    530                 535                 540                 
Leu Glu Leu Pro Thr Asp Ala Phe Val Ile Pro Ala Val Glu Pro Leu 
545                 550                 555                 560 
Leu Ser Pro Leu Leu Ser Val Leu Pro Leu Gln Leu Ile Ser Tyr Tyr 
                565                 570                 575     
Ala Ala Leu Gly Arg Asp Cys Asp Val Asp Lys Pro Arg Asn Leu Ala 
            580                 585                 590         
Lys Ser Val Thr Val Glu 
        595             

<210> 6
<211> 1797
<212> DNA
<213> Exiguobacterium sibiricum 
<223> Es2 Variant

<400> 6
atgtgcggta tcgttggtat gatcggtcag gttaacacca aagaaatcct gctgaaaggt      60

ctggaaaaac tggaataccg tggttacgac tctgctggtc tggctttcgt taacgacggt     120

gttcaggttc acaaagaagt tggtcgtatc gctgctctgc gtgaagttgt tccggctgaa     180

gctgacggtc tggttggtat cggtcacacc cgttgggcta cccacggtgt tccgtctgtt     240

ccgaacgctc acccgcacca gtctgcttct tctcgtttca ccctggttca caacggtgtt     300

atcgaaaacg acgaacagct gaaagctgaa ctgaacgttg acctgctgtc tgacaccgac     360

accgaagtta tcgttcagat gatcgaaaaa aacttcgctg aaaccaacga tgttgttgaa     420

gcgttccgtc agaccctccg tgttctgcac ggttcttacg ctctggctct gatcgacgct     480

gaaaacccgg acgttctgta cgttgctaaa aacaaatctc cgctgctggt tggtctgggt     540

gacggtacct tcaacgttgt tgcttctgac gctatggcta tgctgcaggt taccgaccag     600

ttcgttgaac tgcacgacgg tgaaatgatc atcctgaccc gtgactctgt taccatccag     660

gacctggacg gtaacgttaa agaacgtgaa gcttacaccg ctgaaatcga cgcttctgac     720

atcgaaaaag gtacctacgc tcactacatg ctgaaagaaa tggacgaaca gccggctgtt     780

atccgtaaca tcgttcagaa ataccagaac gaatctggtg aaatcaccct ggaccagtct     840

gttcgtgacc tggttctggg tagggaccgt gtatacatca tcggctgcgg tacctcttac     900

cacgctggtc tgatcggtaa acagctgatc gaacagatcg ctggtatccc gaccgaagtt     960

cacatctctt ctgaattcgg ttacaacatg ccgctgctga ccgaaaaacc gctgttcctg    1020

ttcctgtctc agtctggtga aaccgctgac tctcgtgctg ttctggttga agctaaaaaa    1080

ctgggtcacc cggctctgac catcaccaac gttgctggtt ctaccctgtc tcgtgaagct    1140

aacgctaccc tgctgctgca cgctggtccg gaaatcgctg ttgcttctac caaagcttac    1200

accgctcaga tcgctgttct ggctgttctg gctttcgacc tggctcaggc taaaggtgtt    1260

gacgttaact tcgacctgat gaaagaactg ggtaaaatct cttctgctat ggaatctgtt    1320

atgtctcaga aagaacgttt ccaggaaatc gcttctgaat acctgtctga atctcgtaac    1380

gctttcttca tcggtcgtgg tcaggacgct tacgttggta tggaaggtgc tctgaaactg    1440

aaagaaatct cttacatcca ggctgaaggt tacgctggtg gtgaactgaa acacggtccg    1500

atcgctctga tcgaagacaa caccccggtt atcgctctgg ttacccagcc gcacgttcac    1560

ctgaacaacc gtggtaacgt taaagaagtt gttgctcgtg gtgctaacgc ttgcgttatc    1620

gctgctgaag gtctggaact gccgaccgac gctttcgtta tcccggctgt tgaaccgctg    1680

ctgtctccgc tgctgtctgt tctgccgctg cagctgatct cttactacgc tgctctgggt    1740

cgtgactgcg acgttgacaa accgcgtaac ctggctaaat ctgttaccgt tgaataa       1797


<210> 7
<211> 253
<212> PRT
<213> Clostridium nexile


<400> 7
Met Gly Lys Gln Lys Lys Glu Lys Lys Gly Met Lys Ile Ile Lys Thr 
1               5                   10                  15      
Ala Asp Tyr Asn Glu Met Ser Arg Lys Ala Ala Asn Leu Ile Gly Ala 
            20                  25                  30          
Gln Val Ile Met Lys Pro Asn Cys Ile Leu Gly Leu Ala Thr Gly Ser 
        35                  40                  45              
Ser Pro Ile Gly Thr Tyr Lys Glu Leu Ile Lys Arg Cys Glu Glu Gly 
    50                  55                  60                  
Asp Leu Asp Phe Ser Gln Val Lys Ser Val Asn Leu Asp Glu Tyr Lys 
65                  70                  75                  80  
Gly Leu Pro Arg Asp Asn Asp Gln Ser Tyr Tyr Tyr Phe Met Asn His 
                85                  90                  95      
Asn Leu Phe Asp His Ile Asn Ile Asp Lys Ala Asn Thr His Val Pro 
            100                 105                 110         
Asn Gly Met Glu Pro Asp Ala Ala Lys Glu Cys Ala Asn Tyr Glu Glu 
        115                 120                 125             
Leu Ile Lys Ser Leu Gly Gly Val Asp Leu Gln Leu Leu Gly Leu Gly 
    130                 135                 140                 
His Asn Gly His Ile Gly Phe Asn Glu Pro Ala Glu Glu Phe Asp Lys 
145                 150                 155                 160 
Val Thr His Cys Val Asp Leu Gln Glu Ser Thr Ile Glu Ala Asn Lys 
                165                 170                 175     
Arg Phe Phe Glu Ser Ala Asp Asp Val Pro Arg Gln Ala Tyr Thr Met 
            180                 185                 190         
Gly Ile Gly Thr Ile Met Ser Ala Lys Lys Ile Val Val Val Val Ser 
        195                 200                 205             
Gly Glu Asp Lys Ala Asp Ile Val Lys Arg Ala Phe Ser Gly Pro Val 
    210                 215                 220                 
Thr Pro Ser Val Pro Ala Ser Ile Leu Gln Met His Pro Asp Val Thr 
225                 230                 235                 240 
Val Ile Cys Asp Ala Ala Ala Tyr Ser Lys Val Glu Ala 
                245                 250             

<210> 8
<211> 762
<212> DNA
<213> Clostridium nexile


<400> 8
atgggtaaac agaaaaaaga aaaaaaaggt atgaaaatca tcaaaaccgc tgactacaac      60

gaaatgtctc gtaaagctgc taacctgatc ggtgctcagg ttatcatgaa accgaactgc     120

atcctgggtc tggctaccgg ttcttctccg atcggtacct acaaagaact gatcaaacgt     180

tgcgaagaag gtgacctgga cttctctcag gttaaatctg ttaacctgga cgaatacaaa     240

ggtctgccgc gtgacaacga ccagtcttac tactacttca tgaaccacaa cctgttcgac     300

cacatcaaca tcgacaaagc taacacccac gttccgaacg gtatggaacc ggacgctgct     360

aaagaatgcg ctaactacga agaactgatc aaatctctgg gtggtgttga cctgcagctg     420

ctgggtctgg gtcacaacgg ccatatcggc ttcaacgaac cggcggaaga attcgacaaa     480

gttacccact gcgttgacct gcaggaatct accatcgaag ctaacaaacg tttcttcgaa     540

tctgctgacg acgttccgcg tcaggcttac actatgggta tcggtaccat catgtctgct     600

aaaaaaatcg ttgttgttgt ttctggtgaa gacaaagctg acatcgttaa acgtgctttc     660

tctggtccgg ttaccccgtc tgttccggct tctatcctgc agatgcaccc ggacgttacc     720

gttatctgcg acgctgctgc ttactctaaa gttgaagctt aa                        762


<210> 9
<211> 658
<212> PRT
<213> Dyadobacter fermentans


<400> 9
Met Ser Gln Gln Thr Val Ser Pro Gln Thr Ile Leu Met Gln Ala Ser 
1               5                   10                  15      
Ser Asn Gly Lys Gly Thr Thr Gly Thr Ile Gly Gln Pro Ile Thr Tyr 
            20                  25                  30          
Glu Lys Ile Pro Thr Gln Ile Phe Ala Asp Ser Lys Glu Ala Ser Tyr 
        35                  40                  45              
Ala Val Ala Lys Glu Ile Ser Asp Leu Ile Arg Gln Lys Gln Lys Glu 
    50                  55                  60                  
Gly Lys Pro Cys Val Leu Gly Leu Ala Thr Gly Ser Ser Pro Lys Thr 
65                  70                  75                  80  
Val Tyr Ala Ile Leu Val Arg Met His Arg Glu Glu Gly Leu Ser Phe 
                85                  90                  95      
Lys Asn Val Ile Ser Phe Asn Leu Asp Glu Tyr Tyr Gln Met Glu Pro 
            100                 105                 110         
Asp Ser Ile Tyr Ser Tyr His Arg Phe Met Lys Glu Gln Leu Phe Asp 
        115                 120                 125             
His Val Asp Ile Pro Lys Glu Asn Tyr Phe Leu Pro Asp Gly Thr Val 
    130                 135                 140                 
Pro Ala Ala Leu Leu Arg Asp Tyr Cys Ala Ser Tyr Glu Asn Lys Ile 
145                 150                 155                 160 
Asn Ala Val Gly Gly Leu Asp Phe Gln Leu Leu Gly Ile Gly Gly Asn 
                165                 170                 175     
Gly His Ile Gly Phe Asn Glu Pro Gly Ser Leu Ile Asn Ser His Thr 
            180                 185                 190         
Arg Leu Ile Thr Leu Asp His Ser Thr Arg Ala Ala Ala Ser Met Glu 
        195                 200                 205             
Phe Gly Gly Leu His Asn Val Pro Arg Lys Ala Ile Thr Leu Gly Val 
    210                 215                 220                 
Ala Pro Ile Leu Asn Ala Arg Arg Val Val Leu Leu Ala Trp Gly Glu 
225                 230                 235                 240 
Arg Lys Ala Gln Val Ile Lys Gly Ala Val Glu Gly Pro Val Thr Glu 
                245                 250                 255     
Leu Asn Pro Ala Ser Tyr Leu Gln Ala His Ala Asp Val Ser Phe Val 
            260                 265                 270         
Val Asp Glu Ser Ala Ala Ser Glu Leu Thr Arg Ile Lys Thr Pro Trp 
        275                 280                 285             
Ala Val Asp Ser Val Ile Trp Asp Asn Lys Met Ile Lys Lys Ala Val 
    290                 295                 300                 
Thr His Leu Ser Gln Thr Leu Lys Lys Pro Ile Leu Lys Leu Thr Asp 
305                 310                 315                 320 
Lys Asp Tyr Asn Asp Asn Gly Met Ser Asp Leu Leu Ala Gln Tyr Gly 
                325                 330                 335     
Ala Gly Tyr Glu Ile Asn Ile Asn Val Phe Asn Gln Leu Gln His Thr 
            340                 345                 350         
Ile Thr Gly Trp Pro Gly Gly Lys Pro Asn Ala Asp Asp Thr His Arg 
        355                 360                 365             
Pro Glu Arg Ala Gln Pro Ala Lys Lys Arg Val Leu Ile Phe Ser Pro 
    370                 375                 380                 
His Pro Asp Asp Asp Ile Ile Ser Met Gly Gly Thr Phe Gln Arg Leu 
385                 390                 395                 400 
Val Asp Gln Gly His Glu Val His Val Ala Tyr Gln Thr Ser Gly Asn 
                405                 410                 415     
Ile Ala Val Ala Asp Asp Glu Ala Leu Arg Phe Ile Asp Phe Val Val 
            420                 425                 430         
Asp Phe Asn Thr Gly Phe Asp Ile Glu Ser Pro Asn Ala Asn Lys Leu 
        435                 440                 445             
Phe Leu Asp Ala Lys Glu Phe Leu Arg His Thr Lys Asn Ser Glu Ile 
    450                 455                 460                 
Asp Ser Pro Glu Val Arg Lys Val Lys Gly Leu Ile Arg Arg Gly Glu 
465                 470                 475                 480 
Ala Lys Ala Thr Cys Arg Phe Val Gly Ile Pro Thr Ser Gln Ala His 
                485                 490                 495     
Phe Leu Asp Met Pro Phe Tyr Glu Thr Gly Thr Val Gln Lys Lys Pro 
            500                 505                 510         
Ile Gly Glu Glu Asp Ile Lys Ile Ile Glu Asn Leu Ile Glu Glu Val 
        515                 520                 525             
Lys Pro His Gln Ile Tyr Ala Ala Gly Asp Phe Ala Asp Pro His Gly 
    530                 535                 540                 
Thr His Lys Val Cys Trp Asp Ala Ile Glu Ala Ala Leu Gln Arg Ser 
545                 550                 555                 560 
Lys His Lys Asn Phe Met Lys Asp Cys Trp Val Trp Leu Tyr Arg Gly 
                565                 570                 575     
Ala Trp Ala Glu Trp Asp Ile Tyr Glu Ile Glu Met Ala Val Pro Met 
            580                 585                 590         
Ser Pro Asp Gln Val Leu Lys Lys Arg Gln Gly Ile Phe Lys His Gln 
        595                 600                 605             
Ser Gln Lys Asp Gly Val Val Phe Gln Gly Glu Asp Ser Arg Glu Phe 
    610                 615                 620                 
Trp Gln Arg Ala Glu Glu Arg Asn Arg Gly Thr Ala Gln Leu Tyr Asp 
625                 630                 635                 640 
Ser Leu Gly Leu Ala Glu Tyr Glu Ala Met Glu Ala Phe Val Arg Trp 
                645                 650                 655     
Lys Phe 
        

<210> 10
<211> 1977
<212> DNA
<213> Dyadobacter fermentans


<400> 10
atgtctcagc aaaccgtaag cccgcagacc atcctgatgc aggcttcttc taacggtaaa      60

ggtaccaccg gtaccatcgg tcagccgatc acctacgaaa aaatcccgac ccagatcttc     120

gctgactcta aagaagcttc ttacgctgtt gctaaagaaa tctctgacct gatccgtcag     180

aaacagaaag aaggtaaacc gtgcgttctg ggtctggcta ccggttcttc tcccaagacc     240

gtatacgcaa tcctggttcg tatgcaccgt gaagaaggtc tgtctttcaa aaacgttatc     300

tctttcaacc tggacgaata ctaccagatg gaaccggact ctatctactc ttaccaccgt     360

ttcatgaaag aacagctgtt cgaccacgtt gacatcccga aagaaaacta cttcctgccg     420

gacggtaccg ttccggctgc tctgctgcgt gactactgcg cttcttacga aaacaaaatc     480

aacgctgttg gtggtctgga cttccagctg ctgggtatcg gtggtaacgg ccatatcggc     540

ttcaacgaac cgggctctct gatcaactct cacacccgtc tgatcaccct ggaccactct     600

acccgtgctg ctgcttctat ggaattcggt ggtctgcaca acgttccgcg taaagctatc     660

accctgggtg ttgctccgat cctgaacgct cgtcgtgttg ttctgctggc ttggggtgaa     720

cgtaaagctc aggttatcaa aggtgctgtt gaaggtccgg ttaccgaact gaacccggct     780

tcttacctgc aggctcacgc tgacgtttct ttcgttgttg acgaatctgc tgcttctgaa     840

ctgacccgta tcaaaacccc gtgggctgtt gactctgtta tctgggacaa caaaatgatc     900

aaaaaagctg ttacccacct gtctcagacc ctgaaaaaac cgatcctgaa actgaccgac     960

aaagactaca acgacaacgg tatgtctgac ctgctggctc agtacggtgc tggttacgaa    1020

ataaatatca acgtgttcaa ccaactgcag cacaccatca ccggttggcc gggtggtaaa    1080

ccgaacgctg acgacaccca ccgtccggaa cgtgctcagc cggctaaaaa acgtgttctg    1140

atcttctctc cgcacccgga cgacgacatc atctctatgg gtggtacctt ccagcgtctg    1200

gttgaccagg gtcacgaagt tcacgttgct taccagacct ctggtaacat cgctgttgct    1260

gacgacgaag ctctgcgttt tatcgacttc gttgttgact ttaacaccgg cttcgacatc    1320

gaatcgccga acgctaacaa actgttcctg gacgctaaag aattcctgcg tcacaccaaa    1380

aactctgaaa tcgactctcc ggaagttcgt aaagttaaag gtctgatccg tcgtggtgaa    1440

gctaaagcta cctgccgttt cgttggtatc ccgacctctc aggctcactt cctggacatg    1500

ccgttctacg aaaccggtac cgttcagaaa aaaccgatcg gtgaagaaga catcaaaatc    1560

atcgaaaacc tgatcgaaga agttaaaccg caccagatct acgctgctgg tgacttcgct    1620

gacccgcacg gtacccacaa agtttgctgg gacgctatcg aagctgctct gcagcgttct    1680

aaacacaaaa acttcatgaa agactgctgg gtttggctgt accgtggtgc ttgggctgaa    1740

tgggacatct acgaaatcga aatggctgtt ccgatgtctc cggaccaggt tctgaaaaaa    1800

cgtcagggta tcttcaaaca ccagtctcag aaagacggcg ttgtattcca gggcgaagac    1860

tctcgtgaat tctggcagcg tgctgaagaa cgtaaccgtg gtaccgctca gctgtacgac    1920

tctctgggtc tggctgaata cgaagctatg gaagctttcg ttcgttggaa attctaa       1977


<210> 11
<211> 249
<212> PRT
<213> Pedobacter heparinus
<223> Ph1 variant

<400> 11
Met Thr Ile Lys Met Glu Thr Ser Val Thr Asn Asn Pro Val Glu Leu 
1               5                   10                  15      
Gly Lys Ala Ala Gly Lys Ala Ala Ala Ala Leu Ile Arg Glu Thr Ile 
            20                  25                  30          
Ala Lys Asn Gly Lys Ala Asn Ile Ile Leu Ala Thr Gly Ala Ser Gln 
        35                  40                  45              
Phe Glu Thr Leu Asn Gln Leu Ile Ser Glu Glu Ile Asp Trp Ser Lys 
    50                  55                  60                  
Val Thr Met Phe His Leu Asp Glu Tyr Ile Gly Leu Ser Glu Ser Ala 
65                  70                  75                  80  
Pro Ala Ser Phe Arg Lys Tyr Leu Lys Glu Arg Phe Leu Glu Lys Val 
                85                  90                  95      
Ser Pro Leu Lys Ala Ala Tyr Leu Val Asn Gly Glu Thr Asp Pro Glu 
            100                 105                 110         
Ala Glu Cys Gln Arg Leu Gly Glu Ile Ile Thr Gln Asn Pro Ile Asp 
        115                 120                 125             
Val Ala Leu Val Gly Ile Gly Glu Asn Gly His Leu Ala Phe Asn Asp 
    130                 135                 140                 
Pro Pro Ala Asp Phe Glu Thr Lys Arg Pro Tyr Leu Val Val Asn Leu 
145                 150                 155                 160 
Asp Glu Gln Cys Arg Arg Gln Gln Phe Gly Glu Gly Trp Phe Lys Thr 
                165                 170                 175     
Ile Glu Glu Val Pro Thr Gln Ala Ile Ser Met Ser Val Gln Gln Ile 
            180                 185                 190         
Leu Lys Ser Lys His Ile Ile Cys Ser Val Pro Asp Ser Arg Lys Ala 
        195                 200                 205             
Gln Ala Val Lys Asp Ser Leu Glu Gln Pro Val Ser Asn Gln Tyr Pro 
    210                 215                 220                 
Ser Ser Ile Leu Gln Leu His Pro Asp Cys Arg Phe Phe Phe Asp Lys 
225                 230                 235                 240 
Ala Ser Ala Glu Leu Leu Ser Glu Ala 
                245                 

<210> 12
<211> 750
<212> DNA
<213> Pedobacter heparinus
<223> Ph1 variant

<400> 12
atgaccatca aaatggaaac ctctgttacc aacaacccgg ttgaactggg taaagctgct      60

ggtaaagctg ctgctgctct gatccgtgaa accatcgcta aaaacggtaa agctaacatc     120

atcctggcta ccggtgcttc tcagttcgaa accctgaacc agctgatctc tgaagaaatc     180

gactggtcta aagttaccat gttccacctg gacgaataca tcggtctgtc tgaatctgct     240

ccagcgagct tccgtaaata cctcaaagaa cgtttcctgg aaaaagtttc tccgctgaaa     300

gctgcttacc tggttaacgg tgaaaccgac ccggaagctg aatgccagcg tctgggtgaa     360

atcatcaccc agaacccgat cgacgttgct ctggttggta tcggtgaaaa cggccatctg     420

gcgttcaacg acccgccagc tgacttcgaa accaaacgtc cgtacctggt tgttaacctg     480

gacgaacagt gccgtcgtca gcagttcggt gaaggttggt tcaaaaccat cgaagaagtt     540

ccgacccagg ctatctctat gtctgttcag cagatcctga aatctaaaca catcatctgc     600

tctgttccgg actctcgtaa agctcaggct gttaaagact ctctggagca accggtaagc     660

aaccagtacc cgtcttctat cctgcagctg cacccggact gccgtttctt cttcgacaaa     720

gcttctgctg aactgctgtc tgaagcttaa                                      750


<210> 13
<211> 254
<212> PRT
<213> Pedobacter heparinus
<223> Ph2 variant

<400> 13
Met Ile Lys Glu Phe Leu Lys Asp Lys Leu Lys Val Lys Val Tyr Asn 
1               5                   10                  15      
Thr Arg Gly Glu Met Gly Thr Ser Ala Ala Asp Leu Ala Ala Gln Cys 
            20                  25                  30          
Leu Arg Glu Leu Leu Gln His Lys Asp Glu Val Asn Ile Ile Phe Ala 
        35                  40                  45              
Ala Ala Ala Ser Gln Asn Glu Phe Leu Glu Ala Ile Ala Val Glu Lys 
    50                  55                  60                  
Asp Ile Ala Trp Asn Arg Val Asn Ala Phe His Met Asp Glu Tyr Thr 
65                  70                  75                  80  
Gly Leu Pro Val Thr His Pro Gln Arg Phe Gly Asn Phe Leu Asn Lys 
                85                  90                  95      
Lys Ile Phe Ser Lys Leu Pro Leu Lys Lys Val Tyr Tyr Leu Asn Glu 
            100                 105                 110         
Asp Gly Asn Asp Thr Glu Ala Glu Ser Arg Arg Tyr Ala Ala Leu Leu 
        115                 120                 125             
Glu Lys Met Pro Pro Asp Ile Thr Phe Met Gly Ile Gly Glu Asn Thr 
    130                 135                 140                 
His Leu Ala Phe Asn Asp Pro His Val Ala Asp Phe Asn Asp Pro Val 
145                 150                 155                 160 
Leu Val Lys Ile Ile Asp Leu Asp Glu Pro Cys Lys Val Gln Gln Val 
                165                 170                 175     
His Asp Gly Cys Phe Pro Thr Val Ala Glu Val Pro Ser Leu Ala Tyr 
            180                 185                 190         
Thr Leu Thr Ile Pro Ala Leu Leu Gln Ser Lys Tyr Ile Phe Cys Met 
        195                 200                 205             
Val Pro Gly Lys Asn Lys Ala Gln Ala Val Lys Tyr Thr Leu Asn Glu 
    210                 215                 220                 
Ser Ile Thr Ala Lys Tyr Pro Ser Thr Ser Leu Arg Thr His Ser Asp 
225                 230                 235                 240 
Ala Thr Leu Phe Leu Asp Gln Asp Ser Ala Ser Leu Ile Leu 
                245                 250                 

<210> 14
<211> 765
<212> DNA
<213> Pedobacter heparinus
<223> Ph2 variant

<400> 14
atgatcaaag aattcctgaa agacaaactg aaagttaaag tttacaacac ccgtggtgaa      60

atgggtacct ctgctgctga cctggctgct cagtgcctgc gtgaactgct gcagcacaaa     120

gacgaagtta acatcatctt cgctgctgct gcttctcaga acgaattcct ggaagctatc     180

gctgttgaaa aagacatcgc ttggaaccgt gttaacgctt tccacatgga cgaatacacc     240

ggtctgccag ttacccatcc gcagaggttc ggtaacttcc tcaacaaaaa aatcttctct     300

aaactgccgc tgaaaaaagt ttactacctg aacgaagacg gtaacgacac cgaagctgaa     360

tctcgtcgtt acgctgctct gctggaaaaa atgccgccgg acatcacctt catgggtatc     420

ggtgaaaaca cccacctggc tttcaacgac ccgcacgttg ctgacttcaa cgacccggtt     480

ctggttaaaa tcatcgacct ggacgaaccg tgcaaagttc agcaggttca cgacggttgc     540

ttcccgaccg ttgctgaagt tccgtctctg gcttacaccc tgaccatccc ggctctgctg     600

cagtctaaat acatcttctg catggttccg ggtaaaaaca aagctcaggc tgttaaatac     660

accctgaacg aatctatcac cgctaaatac ccgtctacct ctctgcgtac ccactctgac     720

gctaccctgt tcctggacca ggactctgct tctctgatcc tgtaa                     765


<210> 15
<211> 639
<212> PRT
<213> Pedobacter heparinus
<223> Ph3 variant

<400> 15
Met Ala Arg Leu Asn Leu Leu Glu Glu Thr Arg Phe Glu Lys Leu Pro 
1               5                   10                  15      
Val Ser Val Phe Glu Asn Pro Lys Glu Ala Ser Leu Ser Val Ala His 
            20                  25                  30          
Arg Ile Gly Asn Leu Ile Lys Glu Lys Gln Lys Asn Asn Ala Gln Ala 
        35                  40                  45              
Val Leu Gly Leu Ala Thr Gly Ala Thr Pro Ile Ala Val Tyr Ala Glu 
    50                  55                  60                  
Leu Val Arg Met His Arg Glu Glu Gly Leu Ser Phe Lys Asn Val Val 
65                  70                  75                  80  
Thr Phe Asn Leu Asp Glu Tyr Tyr Pro Met Gln Pro Asn Ala Ala Gln 
                85                  90                  95      
Ser Tyr Val Thr Phe Met Asn Glu Asn Leu Phe Asp His Ile Asp Ile 
            100                 105                 110         
Asp Lys Lys Asn Val Asn Ile Pro Asp Gly Thr Leu Ser Leu Glu Glu 
        115                 120                 125             
Ile Pro Ala Phe Cys Leu Asn Tyr Glu Lys Lys Ile Gly Asp Leu Gly 
    130                 135                 140                 
Gly Leu Asp Ile Gln Ile Leu Gly Ile Gly Arg Thr Gly His Ile Gly 
145                 150                 155                 160 
Phe Asn Glu Pro Gly Ser Ala Pro Asn Ser Gly Thr Arg Leu Val Thr 
                165                 170                 175     
Leu Asp Asp Leu Thr Arg Arg Asp Ala Ala Arg Asp Phe Gly Gly Lys 
            180                 185                 190         
Ser Phe Val Pro Thr Lys Ala Ile Thr Met Gly Val Gly Thr Ile Phe 
        195                 200                 205             
Lys Ala Arg Glu Ile Ile Leu Met Ala Trp Asn Lys Lys Lys Ala Pro 
    210                 215                 220                 
Ile Ile Lys Lys Thr Val Glu Gly Glu Ile Ser Ser Glu Val Pro Ala 
225                 230                 235                 240 
Thr Tyr Leu Gln Leu Ser Asp Asn Val Glu Phe Ile Leu Asp Lys Asp 
                245                 250                 255     
Ala Ala Ser Leu Leu Thr Arg Phe Asp Thr Pro Trp Leu Val Lys Asp 
            260                 265                 270         
Cys Ile Trp Asp Glu Lys Leu Thr Arg Lys Ala Val Ile Trp Leu Ala 
        275                 280                 285             
Asn Thr Leu Lys Lys Pro Val Leu Lys Leu Thr Glu Asp Asp Tyr Asn 
    290                 295                 300                 
Asn Asn Gly Met Ala Gln Leu Ala Val Glu Gln Gly Pro Val Tyr Asn 
305                 310                 315                 320 
Ile Asn Ile His Ile Phe Asn Lys Leu Gln His Thr Ile Thr Gly Trp 
                325                 330                 335     
Pro Gly Gly Lys Pro Asn Ala Asp Asp Ser Gln Arg Pro Glu Arg Ala 
            340                 345                 350         
Glu Pro Ala Lys Lys Arg Ser Ile Ile Phe Ser Pro His Pro Asp Asp 
        355                 360                 365             
Asp Val Ile Ser Met Gly Gly Thr Phe Ile Arg Leu Val Asp Gln Gln 
    370                 375                 380                 
Gln Asp Val His Val Ala Tyr Gln Thr Ser Gly Asn Thr Ala Val Trp 
385                 390                 395                 400 
Asp Asp Asp Ala Leu Arg Phe Val Glu Phe Asn Ile Asp Phe Ser Glu 
                405                 410                 415     
Lys Met Gly Leu Glu Thr Ala Glu Leu Lys Lys Leu Tyr Gln Asn Met 
            420                 425                 430         
Arg Ser Phe Ile Glu Gln Lys Lys Pro Asn Gln Ile Asp Thr Pro Glu 
        435                 440                 445             
Ile Gln Thr Val Lys Gly Leu Ile Arg Lys Gly Glu Ala Ile Ala Gly 
    450                 455                 460                 
Ala Arg Tyr Cys Gly Leu Ala Asp Asp His Ile His Phe Met Ala Leu 
465                 470                 475                 480 
Pro Phe Tyr Glu Ser Gly Lys Ser Gln Lys Asn Pro Val Thr Asp Ala 
                485                 490                 495     
Asp Val Val Leu Thr Met Glu Leu Leu Gln Lys Val Lys Pro His Gln 
            500                 505                 510         
Val Phe Ala Ala Gly Asp Phe Glu Asp Pro His Gly Thr His Ile Val 
        515                 520                 525             
Cys Phe Asn Ile Ile Leu Glu Ala Leu Arg Arg Leu Ser Lys Thr Glu 
    530                 535                 540                 
Ala Trp Val Lys Asp Cys Trp Leu Trp Met Tyr Arg Gly Ala Trp Gln 
545                 550                 555                 560 
Glu Phe Glu Thr Tyr Glu Ile Glu Met Ala Val Pro Leu Ser Pro Gln 
                565                 570                 575     
Glu Val Glu Arg Lys Lys Phe Ala Ile Phe Lys His Gln Ser Gln Lys 
            580                 585                 590         
Asp Arg Ala Val Phe Pro Gly Asp Asp Ala Arg Glu Phe Trp Gln Arg 
        595                 600                 605             
Ala Glu Asp Arg Asn Arg Glu Thr Ala Gln Ala Tyr Asp Ser Leu Gly 
    610                 615                 620                 
Leu Ala Glu Tyr Glu Ala Met Glu Ala Phe Val Arg Tyr Lys Phe 
625                 630                 635                 

<210> 16
<211> 1920
<212> DNA
<213> Pedobacter heparinus
<223> Ph3 variant

<400> 16
atggctcgtc tgaacctgct ggaagaaacc cgtttcgaaa aactgccggt ttctgttttc      60

gaaaacccga aagaagcttc tctgtctgtt gctcaccgta tcggtaacct gatcaaagaa     120

aaacagaaaa acaacgctca ggctgttctg ggtctggcta ccggtgctac cccaatagct     180

gtgtacgcag aactggttcg tatgcaccgt gaagaaggtc tgtctttcaa aaacgttgtt     240

accttcaacc tggacgaata ctacccgatg cagccgaacg ctgctcagtc ttacgttacc     300

ttcatgaacg aaaacctgtt cgaccacatc gacatcgaca aaaaaaacgt taacatcccg     360

gacggtaccc tgtctctgga agaaatcccg gctttctgcc tgaactacga aaaaaaaatc     420

ggtgacctgg gtggtctgga catccagatc ctgggtatcg gtcgtaccgg tcatataggc     480

ttcaacgaac cgggctctgc tccgaactct ggtacccgtc tggttaccct ggacgacctg     540

acccgtcgtg acgctgctcg tgacttcggt ggtaaatctt tcgttccgac caaagctatc     600

actatgggtg ttggtaccat cttcaaagct cgtgaaatca tcctgatggc ttggaacaaa     660

aaaaaagctc cgatcatcaa aaaaaccgtt gaaggtgaaa tctcttctga agttccggct     720

acctacctgc agctgtctga caacgttgaa ttcatcctgg acaaagacgc tgcttctctg     780

ctgacccgtt tcgacacccc gtggctggtt aaagactgca tctgggacga aaaactgacc     840

cgtaaagctg ttatctggct ggctaacacc ctgaaaaaac cggttctgaa actgaccgaa     900

gacgactaca acaacaacgg tatggctcag ctggctgttg aacagggtcc ggtttacaac     960

atcaacatcc acatcttcaa caaactgcag cacaccatca ccggttggcc gggtggtaaa    1020

ccgaacgctg acgactctca gcgtccggaa cgtgctgaac cggctaaaaa acgttctatc    1080

atcttctctc cgcacccgga cgacgacgtt atctctatgg gtggtacctt catccgtctg    1140

gttgaccagc agcaggacgt tcacgttgct taccagacct ctggtaacac cgctgtttgg    1200

gacgacgacg ctctgcgttt cgttgaattc aacatcgact tctctgaaaa aatgggtctg    1260

gaaaccgctg aactgaaaaa actgtaccag aacatgcgta gcttcatcga acagaagaaa    1320

ccgaaccaga tcgacacccc ggaaatccag accgttaaag gtctgatccg taaaggtgaa    1380

gctatcgctg gtgctcgtta ctgcggtctg gctgacgacc acatccactt catggctctg    1440

ccgttctacg aatctggtaa atctcagaaa aacccggtta ccgacgctga cgttgttctg    1500

actatggaac tgctgcagaa agttaaaccg caccaggttt tcgctgctgg tgacttcgaa    1560

gacccgcacg gtacccacat cgtttgcttc aacatcatcc tggaagctct gcgtcgtctg    1620

tctaaaaccg aagcttgggt taaagactgc tggctgtgga tgtaccgtgg tgcttggcag    1680

gaattcgaaa cctacgaaat cgaaatggct gttccgctgt ctccgcagga agttgaacgt    1740

aaaaaattcg ctatcttcaa acaccagtct cagaaagacc gtgctgtttt cccgggtgac    1800

gacgctcgtg aattctggca gcgtgctgaa gaccgtaacc gtgaaaccgc tcaggcttac    1860

gactctctgg gtctggctga atacgaagct atggaagctt tcgttcgtta caaattctaa    1920


<210> 17
<211> 172
<212> PRT
<213> Granulicella tundricola


<400> 17
Met Arg Thr Gly Ser His Gly Trp Ser Asp His Gly Gln Ala Leu Arg 
1               5                   10                  15      
Lys Gln Ala Ile Val Asp Ala Leu Ser Leu Gly Val Ala Lys His His 
            20                  25                  30          
Pro Arg Asp Leu Arg Leu Phe Ala Ala Val Leu Glu Lys Asn Asn Phe 
        35                  40                  45              
Ala Gly Gln Asp Ile Ala Gln Ile Ala Phe Glu Gln Leu Ser Ser Arg 
    50                  55                  60                  
Phe Asp Gln Tyr Leu Gly Arg Leu Tyr Arg Glu Lys Gly Asp Lys Gln 
65                  70                  75                  80  
Arg Gly Leu Ile Leu Phe Asp Lys Ser Ser Thr Glu Arg Arg Ile Gln 
                85                  90                  95      
Thr Leu Ala Arg Asp Phe Lys His Thr Gly His Ser Phe Gly Ile Thr 
            100                 105                 110         
Arg Asn Tyr Ala Glu Val Pro Val Phe Leu Asp Ser Arg Ala Ser Arg 
        115                 120                 125             
Leu Ile Gln Leu Ala Asp Leu Val Ala Tyr Ala Ile Phe Arg His His 
    130                 135                 140                 
Glu His Gly Asp Ser Thr Tyr Tyr Asn Ala Cys Ser His Cys Phe Asp 
145                 150                 155                 160 
Ala Glu Gly Gly Val Val His Gly Leu Tyr Val Arg 
                165                 170         

<210> 18
<211> 519
<212> DNA
<213> Granulicella tundricola


<400> 18
atgcgtaccg gttctcacgg ttggtctgac cacggtcagg ctctgcgtaa acaggctatc      60

gttgacgctc tgtctctggg tgttgctaaa caccacccgc gtgacctgcg tctgttcgct     120

gctgttctgg aaaaaaacaa cttcgctggt caggacatcg ctcagatcgc tttcgaacag     180

ctgtcttctc gtttcgacca gtacctgggt cgtctgtacc gtgaaaaagg tgacaaacag     240

cgtggtctga tcctgttcga caaatcttct accgaacgtc gtatccagac cctggctcgt     300

gacttcaaac acaccggtca ctctttcggt atcaccagaa actacgctga agttccggta     360

ttcctggaca gccgtgcttc tcgtctgatc cagctggctg acctggttgc ttacgctatc     420

ttccgtcacc acgaacacgg tgactctacc tactacaacg cttgctctca ctgcttcgac     480

gctgaaggtg gtgttgttca cggtctgtac gttcgttaa                            519


<210> 19
<211> 629
<212> PRT
<213> Terriglobus roseus


<400> 19
Met Cys Gly Ile Val Gly Tyr Ile Gly Ala Arg Pro Ala Val Pro Val 
1               5                   10                  15      
Ile Met Asp Gly Leu Arg Arg Leu Glu Tyr Arg Gly Tyr Asp Ser Ala 
            20                  25                  30          
Gly Ile Ala Leu Gly Gly Ala Asp Thr Gly Leu Ser Ile Arg Arg Ala 
        35                  40                  45              
Ser Gly Lys Leu Arg Asn Leu Glu Ser Ser Leu Ser Leu Asn Pro Leu 
    50                  55                  60                  
Gln Gly Asp Tyr Gly Ile Gly His Thr Arg Trp Ala Thr His Gly Arg 
65                  70                  75                  80  
Pro Ser Glu Thr Asn Ala His Pro His Arg Asp Cys Thr Gly Ser Leu 
                85                  90                  95      
Val Val Val His Asn Gly Ile Val Glu Asn Tyr Cys Glu Leu Gln Asp 
            100                 105                 110         
Gln Leu Lys Ala Lys Gly His Phe Phe Leu Thr Glu Thr Asp Thr Glu 
        115                 120                 125             
Ile Ile Ala His Leu Ile Glu Gln Glu Tyr Leu Asp Leu Arg Glu Arg 
    130                 135                 140                 
Asn Asp Gly Asp Ala Ala Pro Ser Leu Glu Lys Ala Val Ser Gln Ala 
145                 150                 155                 160 
Val Lys Lys Leu Thr Gly Ala Phe Ala Ile Gly Val Ile Ser Thr Asp 
                165                 170                 175     
Asp Pro Gly Lys Leu Val Ala Ala Arg Ser Gly Pro Pro Ala Val Val 
            180                 185                 190         
Gly Phe Gly Asp Gly Glu Tyr Phe Leu Ala Ser Asp Val Pro Gly Ile 
        195                 200                 205             
Leu Gln Tyr Thr Arg Asp Ile Val Phe Leu Gln Asp Gly Asp Val Ala 
    210                 215                 220                 
Val Leu Thr Arg Ser Gly Ile Ser Phe Ser Asp Phe His Gly Lys Pro 
225                 230                 235                 240 
Leu Lys Arg Ser Pro Gln His Ile Thr Trp Asp Pro Ile Gln Ala Glu 
                245                 250                 255     
Lys Asn Gly Tyr Ala His Phe Met Leu Lys Glu Ile Tyr Glu Gln Pro 
            260                 265                 270         
Arg Ala Val Arg Asp Thr Leu Arg Gly Arg Ile Ser Ala Ala Ser Gly 
        275                 280                 285             
His Ile Glu Leu Pro Gly Leu Asp Leu Ser His Asp Glu Ile Val His 
    290                 295                 300                 
Ala Glu Arg Ile Leu Ile Ala Ser Cys Gly Thr Ser Trp His Ala Gly 
305                 310                 315                 320 
Leu Val Gly Lys Phe Leu Leu Glu Arg Met Val Arg Ile Pro Val Glu 
                325                 330                 335     
Val Asp Tyr Ala Ser Glu Phe Arg Tyr Arg Arg Pro Ile Met Arg Ser 
            340                 345                 350         
Arg Asp Ile Gly Leu Leu Ile Ser Gln Ser Gly Glu Thr Ala Asp Thr 
        355                 360                 365             
Leu Ala Ala Gln Leu Glu Met Ile Ala Gly Asp Ile Pro Thr Leu Ser 
    370                 375                 380                 
Ile Cys Asn Val Val Asp Ala Ala Leu Thr Arg Lys Ala Arg Gly Ser 
385                 390                 395                 400 
Ile Thr Thr Asn Ala Gly Pro Glu Ile Gly Val Ala Ser Thr Lys Ala 
                405                 410                 415     
Phe Thr Ala Gln Ile Ala Ala Leu Leu Thr Leu Ser Leu Tyr Phe Ala 
            420                 425                 430         
Gln Cys Arg Lys Thr Met Pro Gln Glu Ala Val Gln Ser Ile Leu Arg 
        435                 440                 445             
Glu Leu Glu Val Leu Pro Lys Ala Met Glu Glu Ala Leu Arg Thr Ser 
    450                 455                 460                 
Ala Pro Val Cys Ala Arg Leu Ala Lys Ile Phe Ser Asn Ala Thr Asp 
465                 470                 475                 480 
Val Leu Phe Leu Gly Arg Gly Ile His Tyr Pro Ile Ala Leu Glu Gly 
                485                 490                 495     
Ala Leu Lys Leu Lys Glu Ile Ser Tyr Ile His Ala Glu Gly Tyr Pro 
            500                 505                 510         
Ala Gly Glu Met Lys His Gly Pro Asn Ala Leu Ile Asp Glu Thr Leu 
        515                 520                 525             
Pro Val Val Cys Leu Ala Thr Lys Asp Gly Asp Asp Pro Asp Ser Val 
    530                 535                 540                 
Leu Lys Tyr Glu Lys Thr Leu Ser Asn Ile Gln Glu Val Thr Ala Arg 
545                 550                 555                 560 
Gly Gly Arg Val Ile Ala Ile Val Val Glu Gly Asp Thr Ala Val Ser 
                565                 570                 575     
Ser Leu Val Glu His Thr Ile Thr Ile Pro Arg Ala Ser Glu Ala Leu 
            580                 585                 590         
Leu Pro Ile Leu Glu Thr Val Pro Leu Gln Leu Leu Ala Tyr Phe Ile 
        595                 600                 605             
Ala Val Glu Lys Gly Cys Asp Val Asp Gln Pro Arg Asn Leu Ala Lys 
    610                 615                 620                 
Ser Val Thr Val Glu 
625                 

<210> 20
<211> 1890
<212> DNA
<213> Terriglobus roseus


<400> 20
atgtgcggta tcgttggtta catcggtgct cgtccggctg ttccggttat catggacggt      60

ctgcgtcgtc tggaataccg tggttacgac tctgctggta tcgctctggg tggtgctgac     120

accggtctgt ctatccgtcg tgcttctggt aaactgcgta acctggaatc ttctctgtct     180

ctgaacccgc tgcagggtga ctacggtatc ggtcacaccc gttgggctac ccacggtcgt     240

ccgtctgaaa ccaacgctca cccgcaccgt gactgcaccg gttctctggt tgttgttcac     300

aacggtatcg ttgaaaacta ctgcgaactg caggaccagc tgaaagctaa aggtcacttc     360

ttcctgaccg aaaccgacac cgaaatcatc gctcacctga tcgaacagga atacctggac     420

ctgcgtgaac gtaacgacgg tgacgctgct ccgtctctgg aaaaagctgt ttctcaggct     480

gttaaaaaac tgaccggtgc tttcgctatc ggtgttatct ctaccgacga cccgggtaaa     540

ctggttgctg ctcgttctgg tccgccggct gttgttggtt tcggtgacgg tgaatacttc     600

ctggcttctg acgttccggg tatcctgcag tacactcgtg acatcgtctt cctgcaagac     660

ggtgacgttg ctgttctgac ccgttctggt atctctttct ctgacttcca cggtaaaccg     720

ctgaaacgtt ctccgcagca catcacctgg gacccgatcc aggctgaaaa aaacggttac     780

gctcacttca tgctgaaaga aatctacgaa cagccgcgtg ctgttcgtga caccctgcgt     840

ggtcgtatct ctgctgcttc tggtcacatc gaactgccgg gtctggacct gtctcacgac     900

gaaatcgttc acgctgaacg tatcctgatc gcttcttgcg gtacctcttg gcacgctggt     960

ctggttggta aattcctgct ggaacgtatg gttcgtatcc cggttgaagt tgactacgct    1020

tctgaattcc gttaccgtcg tccgatcatg cgttctcgtg acatcggtct gctgatctct    1080

cagtctggtg aaaccgctga caccctggct gctcagctgg aaatgatcgc tggtgacatc    1140

ccgaccctgt ctatctgcaa cgttgttgac gctgctctga cccgtaaagc tcgtggttct    1200

atcaccacca acgctggtcc ggaaatcggt gttgcttcta ctaaggcgtt caccgctcag    1260

atagctgctc tgctgaccct gtctctgtac ttcgctcagt gccgtaaaac catgccgcag    1320

gaagctgttc agtctatcct gcgtgaactg gaagttctgc cgaaagctat ggaagaagct    1380

ctgcgtacct ctgctccggt ttgcgctcgt ctggctaaaa tcttctctaa cgctaccgac    1440

gttctgttcc tgggtcgtgg tatccactac ccgatcgctc tggaaggtgc tctgaaactg    1500

aaagaaatct cttacatcca cgctgaaggt tacccggctg gtgaaatgaa acacggtccg    1560

aacgctctga tcgacgaaac cctgccggtt gtttgcctgg ctaccaaaga cggtgacgac    1620

ccggactctg ttctgaaata cgaaaaaacc ctgtctaaca tccaggaagt taccgctcgt    1680

ggtggtcgtg ttatcgctat cgttgttgaa ggtgacaccg ctgtttcttc tctggttgaa    1740

cacaccatca ccatcccgcg tgcttctgaa gctctgctgc cgatcctgga aaccgttccg    1800

ctgcagctgc tggcttactt catcgctgtt gaaaaaggtt gcgacgttga ccagccgcgt    1860

aacctggcta aatctgttac cgttgaataa                                     1890


<210> 21
<211> 294
<212> PRT
<213> Akkermansia muciniphila


<400> 21
Met Arg Ala Ala Pro Arg Lys Ala Val Gly Arg Val Glu Ala Ser Val 
1               5                   10                  15      
Pro Trp Asp Phe Ser Arg Lys Tyr Ala Ala Val His His Ser Gly Thr 
            20                  25                  30          
Ile Ser Val Glu Asn Ala Arg Lys Arg Ser Lys Ile Gly Arg Val Met 
        35                  40                  45              
Lys Val Glu Thr Phe Glu Thr Pro Gln Asp Ala Ala Lys Ala Leu Ala 
    50                  55                  60                  
Gly Glu Val Ala Glu Leu Ile Arg Thr Arg Ala Ala Glu Gly Lys Asn 
65                  70                  75                  80  
Val Val Leu Gly Leu Ala Thr Gly Ala Thr Pro Leu Pro Phe Tyr Ala 
                85                  90                  95      
Glu Leu Val Arg Met His Lys Glu Glu Gly Leu Ser Phe Ala Asn Val 
            100                 105                 110         
Ile Ser Phe Asn Leu Asp Glu Tyr Ser Gly Leu Asp Arg Asp His Pro 
        115                 120                 125             
Glu Ser Tyr Trp Tyr Phe Met His Thr Asn Leu Phe Asn His Ile Asp 
    130                 135                 140                 
Ile Lys Pro Glu Asn Ile Asn Leu Pro Ser Gly Thr Val Lys Asp Asp 
145                 150                 155                 160 
Glu Ile Ala Ala His Cys Ala Ala Tyr Glu Gln Lys Ile Lys Asp Cys 
                165                 170                 175     
Gly Gly Ile Asp Leu Gln Ile Leu Gly Ile Gly Arg Thr Gly His Ile 
            180                 185                 190         
Gly Phe Asn Glu Pro Gly Ser Asp Asp Thr Thr Val Thr Arg Gln Val 
        195                 200                 205             
His Leu Asp Glu Leu Thr Arg Ser Asp Ala Ala Pro Ala Phe Gly Gly 
    210                 215                 220                 
Ile Glu Asn Val Pro Thr Thr Ala Ile Thr Met Gly Val Ala Thr Ile 
225                 230                 235                 240 
Met Gly Ala Arg Glu Val Ala Leu Met Ala Trp Gly Glu Lys Lys Ala 
                245                 250                 255     
Ser Ile Val Lys Lys Ala Val Gln Gly Pro Val Thr Val Asp Val Ala 
            260                 265                 270         
Ala Ser Tyr Leu Gln Lys His Pro Asn Ala Lys Phe Leu Leu Asp Lys 
        275                 280                 285             
Gly Ala Ala Ser Leu Leu 
    290                 

<210> 22
<211> 885
<212> DNA
<213> Akkermansia muciniphila


<400> 22
atgcgtgctg ctccgcgtaa agctgttggt cgtgttgaag cttctgttcc gtgggacttc      60

tctcgtaaat acgctgctgt tcaccactct ggtaccatct ctgttgaaaa cgctcgtaaa     120

cgttctaaaa tcggtcgtgt tatgaaagtt gaaaccttcg aaaccccgca ggacgctgct     180

aaagctctgg ctggtgaagt tgctgaactg atccgtaccc gtgctgctga aggtaaaaac     240

gttgttctgg gtctggctac cggtgctacc ccgctgccgt tctacgctga actggttcgt     300

atgcacaaag aagaaggtct gtctttcgct aacgttatct ctttcaacct ggacgaatac     360

tctggtctgg accgtgacca cccggaatct tactggtact tcatgcacac caacctgttc     420

aaccacatcg acatcaaacc ggaaaacatc aacctgccgt ctggtaccgt taaagacgac     480

gaaatcgctg ctcactgcgc tgcttacgaa cagaaaatca aagactgcgg tggtatcgac     540

ctgcagatcc tgggtatcgg tcgtaccggt catataggct tcaacgaacc gggctctgac     600

gacaccaccg ttacccgtca ggttcacctg gacgaactga cccgttctga cgctgcccct     660

gcgttcggtg gtatcgagaa cgttccgacc accgctatca ctatgggtgt tgctaccatc     720

atgggtgctc gtgaagttgc tctgatggct tggggtgaaa aaaaagcttc tatcgttaaa     780

aaagctgttc agggtccggt taccgttgac gttgctgctt cttacctgca gaaacacccg     840

aacgctaaat tcctgctgga caaaggtgct gcttctctgc tgtaa                     885


<210> 23
<211> 270
<212> PRT
<213> Bifidobacterium longum
<223> Bl1 variant

<400> 23
Met Pro Glu Ile Ile Ile Val Lys Asn Glu Ala Glu Ala Gly Glu Ile 
1               5                   10                  15      
Tyr Gly Arg Cys Val Ala Asp Leu Ile Lys Ala Lys Pro Asp Ala Val 
            20                  25                  30          
Leu Gly Leu Ala Thr Gly Ser Ser Pro Leu Ala Ala Tyr Gln Ala Leu 
        35                  40                  45              
Ala Lys Ile Val Lys Asp Glu Ala Ile Asp Val Ser Gly Val Arg Gly 
    50                  55                  60                  
Phe Ala Leu Asp Glu Tyr Ile Gly Leu Pro Leu Thr His Pro Glu Ser 
65                  70                  75                  80  
Tyr His Ala Thr Ile His Arg Thr Val Val Glu Pro Leu Gly Leu Asp 
                85                  90                  95      
Pro Ala Lys Val His Val Pro Gly Asp Val Leu Asn Gly Thr Pro Leu 
            100                 105                 110         
Glu Asp Gly Asp Lys Val Ala Leu Ala Gly Pro Ala Tyr Asp Arg Ala 
        115                 120                 125             
Ile Glu Ala Ala Gly Gly Ile Asp Val Gln Ile Leu Gly Ile Gly Thr 
    130                 135                 140                 
Asp Gly His Val Gly Phe Asn Glu Pro Gly Ser Ser Leu Ala Ser Gly 
145                 150                 155                 160 
Thr Arg Val Lys Thr Leu Ala Glu Gln Thr Arg Val Asp Asn Ala Arg 
                165                 170                 175     
Phe Phe Asp Asn Asp Ile Asn Gln Val Pro Thr His Cys Ile Thr Gln 
            180                 185                 190         
Gly Ile Gly Thr Ile Met Lys Ala Arg His Leu Val Leu Leu Ala Phe 
        195                 200                 205             
Gly Ala Gly Lys Ala Glu Ala Ile Glu Glu Thr Val Glu Gly Gly Leu 
    210                 215                 220                 
Ser Ala Phe Cys Pro Ala Ser Ala Leu Gln Met His Pro His Ala Thr 
225                 230                 235                 240 
Ile Ile Val Asp Glu Glu Ala Ala Ser Arg Leu Arg His Lys Asp Tyr 
                245                 250                 255     
Tyr Arg Tyr Ala Tyr Thr His Lys Pro Ala Trp Gln Gly Ile 
            260                 265                 270 

<210> 24
<211> 813
<212> DNA
<213> Bifidobacterium longum
<223> Bl1 variant

<400> 24
atgccggaaa tcatcatcgt taaaaacgaa gctgaagctg gtgaaatcta cggtcgttgc      60

gttgctgacc tgatcaaagc taaaccggac gctgttctgg gtctggctac cggttcttct     120

ccgctggctg cttaccaggc tctggctaaa atcgttaaag acgaagctat cgacgtttct     180

ggtgttcgtg gtttcgctct ggacgaatac atcggtctgc cgctgaccca cccggaatct     240

taccacgcta ccatccaccg taccgttgtt gaaccgctgg gtctggaccc ggctaaagtt     300

cacgttccgg gtgacgttct gaacggtacc ccgctggaag acggtgacaa agttgctctg     360

gctggtccgg cttacgaccg tgctatcgaa gctgctggtg gtatcgacgt tcagatcctg     420

ggtatcggta ccgacggtca tgtaggcttc aacgaaccgg gctcttctct ggcttctggt     480

acccgtgtta aaaccctggc tgaacagacc cgtgttgaca atgcgaggtt cttcgacaac     540

gacatcaacc aggttccgac ccactgcatc acccagggta tcggtaccat catgaaagct     600

cgtcacctgg ttctgctggc tttcggtgct ggtaaagctg aagctatcga agaaaccgtt     660

gaaggtggtc tgtctgcttt ctgcccggct tctgctctgc agatgcaccc gcacgctacc     720

atcatcgttg acgaagaagc tgcttctcgt ctgcgtcaca aagactacta ccgttacgct     780

tacacccaca aaccggcttg gcagggtatc taa                                  813


<210> 25
<211> 270
<212> PRT
<213> Bifidobacterium longum
<223> Bl2 variant

<400> 25
Met Pro Glu Ile Ile Ile Val Lys Asn Glu Ala Glu Ala Gly Glu Ile 
1               5                   10                  15      
Tyr Gly Arg Cys Val Ala Asp Leu Ile Lys Ala Lys Pro Asp Ala Val 
            20                  25                  30          
Leu Gly Leu Ala Thr Gly Ser Ser Pro Leu Ala Ala Tyr Gln Ala Leu 
        35                  40                  45              
Ala Lys Ile Val Lys Asp Glu Ala Ile Asp Val Ser Gly Val Arg Gly 
    50                  55                  60                  
Phe Ala Leu Asp Glu Tyr Ile Gly Leu Pro Leu Thr His Pro Glu Ser 
65                  70                  75                  80  
Tyr His Ala Thr Ile His Arg Thr Val Val Glu Pro Leu Gly Leu Asp 
                85                  90                  95      
Pro Ala Lys Val His Val Pro Gly Asp Val Leu Asn Gly Thr Pro Leu 
            100                 105                 110         
Glu Asp Gly Asp Lys Val Ala Leu Ala Gly Pro Ala Tyr Asp Arg Ala 
        115                 120                 125             
Ile Glu Ala Ala Gly Gly Ile Asp Val Gln Ile Leu Gly Ile Gly Thr 
    130                 135                 140                 
Asp Gly His Val Gly Phe Asn Glu Pro Gly Ser Ser Leu Ala Ser Gly 
145                 150                 155                 160 
Thr Arg Val Lys Thr Leu Ala Glu Gln Thr Arg Val Asp Asn Ala Arg 
                165                 170                 175     
Phe Phe Asp Asn Asp Ile Asn Gln Val Pro Thr His Cys Ile Thr Gln 
            180                 185                 190         
Gly Ile Gly Thr Ile Met Lys Ala Arg His Leu Val Leu Leu Ala Phe 
        195                 200                 205             
Gly Ala Gly Lys Ala Glu Ala Ile Glu Glu Thr Val Glu Gly Gly Leu 
    210                 215                 220                 
Ser Ala Phe Cys Pro Ala Ser Ala Leu Gln Met His Pro His Ala Thr 
225                 230                 235                 240 
Ile Ile Val Asp Glu Glu Ala Ala Ser Arg Leu Arg His Lys Asp Tyr 
                245                 250                 255     
Tyr Arg Tyr Ala Tyr Thr His Lys Pro Ala Trp Gln Gly Ile 
            260                 265                 270 

<210> 26
<211> 813
<212> DNA
<213> Bifidobacterium longum
<223> Bl2 variant

<400> 26
atgccggaaa tcatcatcgt taaaaacgaa gctgaagctg gtgaaatcta cggtcgttgc      60

gttgctgacc tgatcaaagc taaaccggac gctgttctgg gtctggctac cggttcttct     120

ccgctggctg cttaccaggc tctggctaaa atcgttaaag acgaagctat cgacgtttct     180

ggtgttcgtg gtttcgctct ggacgaatac atcggtctgc cgctgaccca cccggaatct     240

taccacgcta ccatccaccg taccgttgtt gaaccgctgg gtctggaccc ggctaaagtt     300

cacgttccgg gtgacgttct gaacggtacc ccgctggaag acggtgacaa agttgctctg     360

gctggtccgg cttacgaccg tgctatcgaa gctgctggtg gtatcgacgt tcagatcctg     420

ggtatcggta ccgacggtca tgtaggcttc aacgaaccgg gctcttctct ggcttctggt     480

acccgtgtta aaaccctggc tgaacagacc cgtgttgaca atgcgaggtt cttcgacaac     540

gacatcaacc aggttccgac ccactgcatc acccagggta tcggtaccat catgaaagct     600

cgtcacctgg ttctgctggc tttcggtgct ggtaaagctg aagctatcga agaaaccgtt     660

gaaggtggtc tgtctgcttt ctgcccggct tctgctctgc agatgcaccc gcacgctacc     720

atcatcgttg acgaagaagc tgcttctcgt ctgcgtcaca aagactacta ccgttacgct     780

tacacccaca aaccggcttg gcagggtatc taa                                  813


<210> 27
<211> 242
<212> PRT
<213> Myxobacterium xanthis


<400> 27
Met Arg Ile Arg Val Phe Asp Ser Glu Arg Glu Ala Ala Ala Thr Cys 
1               5                   10                  15      
Ala Gln Arg Ile Ala Arg Ala Ala Ala Ala His Pro Ser Leu Val Leu 
            20                  25                  30          
Gly Leu Pro Thr Gly Arg Thr Pro Leu Asn Val Tyr Arg Glu Leu Val 
        35                  40                  45              
Glu Leu Phe Thr Arg Gly Gly Leu Asp Trp Ala Gln Val Arg Thr Phe 
    50                  55                  60                  
Asn Leu Asp Glu Phe Leu Gly Val Ser Ala Asp Asp Ala Gly Ser Phe 
65                  70                  75                  80  
Arg Ala Tyr Met Glu Arg His Leu Phe Gln His Val Asn Leu Ser Pro 
                85                  90                  95      
Ala His Ile Gln Phe Leu Asp Gly Ala Val Glu Asp Ala Glu Ala Glu 
            100                 105                 110         
Cys Ala Arg Tyr Glu Ala Arg Leu Ala Glu Ala Gly Gly Leu Asp Leu 
        115                 120                 125             
Val Leu Leu Gly Leu Gly Ser Asn Gly His Leu Ala Phe Asn Glu Pro 
    130                 135                 140                 
Ala Asp Gly Leu Arg Ala Arg Cys His Arg Thr Arg Leu Ser Arg Gln 
145                 150                 155                 160 
Thr Arg Glu Ala Asn Leu Met Leu Phe Gly Asp Asp Pro Ser Arg Val 
                165                 170                 175     
Pro Met Glu Ala Leu Thr Leu Gly Met Ala Ser Ile Leu Gln Ala Arg 
            180                 185                 190         
Gln Ala Leu Leu Leu Ala Phe Gly Glu Ala Lys Ala Glu Ala Val Arg 
        195                 200                 205             
Gly Met Val Glu Gly Pro Val Ser Pro Arg Cys Pro Ala Ser Phe Leu 
    210                 215                 220                 
Gln Leu His Pro Asp Val Glu Val Trp Leu Asp Pro Ala Ala Ala Arg 
225                 230                 235                 240 
Ala Leu 
        

<210> 28
<211> 729
<212> DNA
<213> Myxobacterium xanthis


<400> 28
atgcgtatcc gtgttttcga ctctgaacgt gaagctgctg ctacctgcgc tcagcgtatc      60

gctcgtgctg ctgctgctca cccgtctctg gttctgggtc tgccgaccgg tcgtaccccg     120

ctgaacgttt accgtgaact ggttgaactg ttcacccgtg gtggtctgga ctgggctcag     180

gttcgtacct tcaacctgga cgaatttctc ggtgtaagcg ctgacgacgc tggttctttc     240

cgtgcttaca tggaacgtca cctgttccag cacgttaacc tgtctccggc tcacatccag     300

ttcctggacg gtgctgttga agacgctgaa gctgaatgcg ctcgttacga agctcgtctg     360

gctgaagctg gtggtctgga cctggttctg ctgggtctgg gttctaacgg tcacctggct     420

ttcaacgaac cggctgacgg tctgcgtgct cgttgccacc gtacccgtct gtctcgtcag     480

acccgtgaag ctaacctgat gctgttcggt gacgacccgt ctcgtgttcc gatggaagct     540

ctgaccctgg gtatggcttc tatcctgcag gctcgtcagg ctctgctgct ggctttcggt     600

gaagctaaag ctgaagctgt tcgtggtatg gttgaaggtc cggtttctcc gcgttgccca     660

gctagcttcc tgcagctgca tccggacgtt gaagtttggc tggacccggc tgctgctcgt     720

gctctgtaa                                                             729


<210> 29
<211> 369
<212> PRT
<213> Stackebrandtia nassauensis
<223> Sn1 variant

<400> 29
Met Ser Gln Gln Gln Ala Ala Arg Ser Thr Thr Ala Gly Ala Thr Asp 
1               5                   10                  15      
Ala Thr Ala Ala Thr Pro Pro Gly Ala Gly Met Ala Ala Asp Ile Ala 
            20                  25                  30          
Asp Gln Pro Glu Ala Phe Ala Arg Leu Ala Glu Thr Gln Ser Gly Ala 
        35                  40                  45              
Ile Ala Asp Ile Ala Ala Glu Ile Ala Arg Glu Arg Pro Arg Phe Val 
    50                  55                  60                  
Met Phe Thr Ala Arg Gly Thr Ser Asp His Ala Ala Leu Tyr Ala Ala 
65                  70                  75                  80  
Tyr Leu Thr Glu Ile Arg Leu Gly Ile Pro Ala Gly Leu Ala Ser Pro 
                85                  90                  95      
Ser Ala Ile Thr Val Tyr Gly Ala Arg Pro Asp Leu Ser Glu Ala Leu 
            100                 105                 110         
Val Val Gly Val Ser Gln Ser Gly Gly Ser Pro Asp Ile Ala Glu Val 
        115                 120                 125             
Leu Arg Val Ala Ser Glu Ser Gly Ala Arg Thr Leu Ala Val Thr Asn 
    130                 135                 140                 
Asn Pro Glu Ser Pro Leu Ala Lys Gln Ala Gly Leu Asn Ile Asp Val 
145                 150                 155                 160 
Ser Ala Gly His Glu Lys Ala Val Ala Ala Thr Lys Thr Tyr Thr Ala 
                165                 170                 175     
Glu Leu Leu Ala Leu Phe Met Leu Ile Glu Gly Ile Arg Ala Gly Asp 
            180                 185                 190         
Gly Lys Leu Ala Gly Asp Glu Ala Ala Ala Val Ala Ala Leu Pro Glu 
        195                 200                 205             
Leu Ala Arg Thr Val Leu Ala Asp Glu Thr Pro Val Gln Leu Ala Ala 
    210                 215                 220                 
Arg Tyr Arg Phe Ala Glu Arg Leu Val Thr Thr Gly Arg Gly Tyr Ala 
225                 230                 235                 240 
Tyr Pro Thr Ala Arg Glu Thr Ala Leu Lys Leu Met Glu Thr Ser Tyr 
                245                 250                 255     
Leu Ser Ala Leu Ser Phe Ser Gly Ala Asp Leu Leu His Gly Pro Leu 
            260                 265                 270         
Ala Met Ala Asp Pro Asp Ile Pro Val Leu Ala Ile Val Gly Asp Gly 
        275                 280                 285             
Pro Gly Gly Asn Ala Met Arg Asp Val Ile Gly Arg Leu Gly Glu Arg 
    290                 295                 300                 
Arg Ala Asp Val Val Ser Ile Gly Ala Ser Asp Val Glu Gly Ala Pro 
305                 310                 315                 320 
Ile Arg Ile Pro Val Pro Ala Val Asp Glu Arg Leu Ala Pro Met Leu 
                325                 330                 335     
Asp Ile Leu Pro Leu Gln Arg Leu Ala Leu Ala Leu Ala Leu Asn Arg 
            340                 345                 350         
Gly Glu Asn Pro Asp Ala Pro Arg Gly Leu Arg Lys Val Thr Glu Thr 
        355                 360                 365             
Leu 
    

<210> 30
<211> 1110
<212> DNA
<213> Stackebrandtia nassauensis
<223> Sn1 variant

<400> 30
atgtctcagc agcaggctgc tcgttctacc accgctggtg ctaccgacgc taccgctgct      60

accccgccgg gtgctggtat ggctgctgac atcgctgacc agccggaagc tttcgctcgt     120

ctggctgaaa cccagtctgg tgctatcgct gacatcgctg ctgaaatcgc tcgtgaacgt     180

ccgcgtttcg ttatgttcac cgctcgtggt acctctgacc acgctgctct gtacgctgct     240

tacctgaccg aaatccgtct gggtatcccg gctggtctgg cttctccgtc tgctatcacc     300

gtttacggtg ctcgtccgga cctgtctgaa gctctggttg ttggtgtttc tcagtctggt     360

ggttctccgg acatcgctga agttctgcgt gttgcttctg aatctggtgc tcgtaccctg     420

gctgttacca acaacccgga atctccgctg gctaaacagg ctggtctgaa catcgacgtt     480

tctgctggtc acgaaaaagc tgttgctgct accaaaacct acaccgctga actgctggct     540

ctgttcatgc tgatcgaagg tatccgtgct ggtgacggta aactggctgg tgacgaagct     600

gctgctgttg ctgctctgcc ggaactggct cgtaccgttc tggctgacga aaccccggtt     660

cagctggctg ctcgttaccg tttcgctgaa cgtctggtta ccaccggtcg tggttacgct     720

tacccgaccg ctcgtgaaac cgctctgaaa ctgatggaaa cctcttacct gtctgctctg     780

tctttctctg gtgctgacct gctgcacggt ccgctggcta tggctgaccc ggacatcccg     840

gttctggcta tcgttggtga cggtccgggt ggtaacgcta tgcgtgacgt tatcggtcgt     900

ctgggtgaac gtagggctga cgttgtaagc atcggtgctt ctgacgttga aggtgctccg     960

atccgtatcc cggttccggc tgttgacgaa cgtctggctc cgatgctgga catcctgccg    1020

ctgcagcgtc tggctctggc tctggctctg aaccgtggtg aaaacccgga cgctccgcgt    1080

ggtctgcgta aagttaccga aaccctgtaa                                     1110


<210> 31
<211> 289
<212> PRT
<213> Stackebrandtia nassauensis
<223> Sn2 variant

<400> 31
Met Ala Ile Val Asp Ala Glu Ile Ala Arg Gln Pro Glu Cys Trp Thr 
1               5                   10                  15      
Ala Ala Ala Glu Leu Ala Val Glu His Ala Gly Leu Phe Pro Lys Ala 
            20                  25                  30          
Gly Glu Arg Val Ala Val Val Gly Cys Gly Thr Ser Trp Phe Met Ala 
        35                  40                  45              
Gln Ala Val Ala Gly Leu Arg Glu Ala Ser Gly Ala Gly Glu Thr Asp 
    50                  55                  60                  
Ala Phe Thr Ala Ser Glu Phe Pro Asp Arg Arg Tyr Asp Arg Leu Ile 
65                  70                  75                  80  
Ala Leu Ser Arg Ser Gly Thr Thr Ser Glu Val Val Glu Leu Leu Asn 
                85                  90                  95      
Arg Thr Asp Ile Pro Ser Leu Ala Ile Val Gly Asp Gly Asp Thr Pro 
            100                 105                 110         
Ala Val Ala Ala Ala Asp Ala Ala Ile Ala Met Pro Phe Ala Ser Asp 
        115                 120                 125             
Gln Ser Val Val Met Thr Ser Phe Ala Thr Ser Val Val Ala Leu Phe 
    130                 135                 140                 
Arg Ala Gln Leu Gly Ile Asp Gln Ala Ala Ala Ile Ala Asp Cys Arg 
145                 150                 155                 160 
Thr Ala Leu Thr Ala Glu Leu Pro Val Asp Val Ala Lys Leu Glu Gln 
                165                 170                 175     
Ile Ser Phe Ile Gly Ala Gly Trp Thr Ile Gly Leu Ala Asn Glu Ala 
            180                 185                 190         
Ala Leu Lys Ala Arg Glu Ala Ala Thr Phe Trp Ala Glu Ser Tyr Pro 
        195                 200                 205             
Ala Met Asp Tyr Arg His Gly Pro Ile Ser Ile Ala Gly Pro Asn Arg 
    210                 215                 220                 
Ala Thr Trp Glu Leu Gly Pro Thr Gly Ala Gly Leu Arg Ala Glu Val 
225                 230                 235                 240 
Glu Ala Thr Gly Gly Ala Phe Val Ala Ser Ser Leu Asp Pro Leu Ala 
                245                 250                 255     
Glu Leu Val Leu Ala Gln Arg Leu Ala Val Ala Leu Ala Arg Leu Arg 
            260                 265                 270         
Gly Leu Asn Pro Asp Ser Pro Arg His Leu Thr Arg Ser Val Val Leu 
        275                 280                 285             
Pro 
    

<210> 32
<211> 870
<212> DNA
<213> Stackebrandtia nassauensis
<223> Sn2 variant

<400> 32
atggctatcg ttgacgctga aatcgctcgt cagccggaat gctggaccgc tgctgctgaa      60

ctggctgttg aacacgctgg tctgttcccg aaagctggtg aacgtgttgc tgttgttggt     120

tgcggtacct cttggttcat ggctcaggct gttgctggtc tgcgtgaagc ttctggtgct     180

ggtgaaactg acgcgttcac cgcttctgag ttcccggacc gtcgttacga ccgtctgatc     240

gctctgtctc gttctggtac cacctctgaa gttgttgaac tgctgaaccg taccgacatc     300

ccgtctctgg ctatcgttgg tgacggtgac accccggctg ttgctgctgc tgacgctgct     360

atcgctatgc cgttcgcttc tgaccagtct gttgttatga cctctttcgc tacctctgtt     420

gttgctctgt tccgtgctca gctgggtatc gaccaggctg ctgctatcgc tgactgccgt     480

accgctctga ccgctgaact gccggttgac gttgctaaac tggaacagat ctctttcatc     540

ggtgctggtt ggaccatcgg tctggctaac gaagctgctc tgaaagctcg tgaagctgct     600

accttctggg ctgaatctta cccggctatg gactaccgtc acggtccgat ctctatcgct     660

ggtccgaacc gtgctacctg ggaactgggt ccgaccggtg ctggtctgcg tgctgaagtt     720

gaagctaccg gtggtgcttt cgttgcttct tctctggacc cgctggctga actggttctg     780

gctcagcgtc tggctgttgc tctggctcgt ctgcgtggtc tgaacccgga ctctccgcgt     840

cacctgaccc gttctgttgt tctgccgtaa                                      870


<210> 33
<211> 233
<212> PRT
<213> Artificial Sequence

<220>
<223> Ef-Ser74Ala


<400> 33
Met Gln Ile Ile Arg Val Ala Asn Ala Glu Glu Gly Gly Lys Lys Ala 
1               5                   10                  15      
Phe Glu Leu Ile Lys Glu Gly Met Asn Asn Gly Ala Lys Val Leu Gly 
            20                  25                  30          
Leu Ala Thr Gly Ser Thr Pro Glu Thr Leu Tyr Lys Glu Met Thr Ala 
        35                  40                  45              
Ser Asp Ile Asp Phe Thr Glu Met Thr Ser Val Asn Leu Asp Glu Tyr 
    50                  55                  60                  
Val Gly Leu Gly Gly Glu Asp Glu Gln Ser Tyr Arg Tyr Phe Met Asn 
65                  70                  75                  80  
Lys His Leu Phe Asp Lys Lys Pro Phe Lys Glu Thr Phe Val Pro Asn 
                85                  90                  95      
Gly Lys Ala Glu Asp Leu Asp Ala Ala Ser Ala Glu Tyr Glu Lys Ile 
            100                 105                 110         
Ile Asp Ala His Pro Val Asp Ile Gln Ile Leu Gly Ile Gly Gln Asn 
        115                 120                 125             
Gly His Ile Gly Phe Asn Glu Pro Gly Thr Pro Leu Asp Ser Leu Thr 
    130                 135                 140                 
His Val Val Glu Leu Thr Glu Ser Thr Ile Asn Ala Asn Lys Arg Tyr 
145                 150                 155                 160 
Phe Asp Lys Val Glu Asp Val Pro Thr Arg Ala Val Ser Met Gly Ile 
                165                 170                 175     
Gly Ser Ile Met Lys Gly Lys Lys Met Ile Leu Met Ala Tyr Gly Glu 
            180                 185                 190         
Ala Lys Ala Glu Ala Ile Lys Gly Met Ile Asp Gly Pro Val Thr Thr 
        195                 200                 205             
Asp Met Pro Ala Ser Ala Leu Gln Asn His Gln Asp Val Val Val Ile 
    210                 215                 220                 
Ile Asp Asp Ala Ala Ala Ser Lys Leu 
225                 230             

<210> 34
<211> 702
<212> DNA
<213> Artificial Sequence

<220>
<223> Ef-Ser74Ala


<400> 34
atgcagatca tccgtgttgc taacgctgaa gaaggtggta aaaaagcttt cgaactgatc      60

aaagaaggta tgaacaacgg tgctaaagtt ctgggtctgg ctaccggttc taccccggaa     120

accctgtaca aagaaatgac cgcttctgac atcgacttca ccgaaatgac ctctgttaac     180

ctggacgaat acgttggtct gggtggtgaa gacgaacagt cttaccgtta cttcatgaac     240

aaacacctgt tcgacaaaaa accgttcaaa gaaaccttcg ttccgaacgg taaagctgaa     300

gacctggacg ctgcttctgc tgaatacgaa aaaatcatcg acgctcaccc ggttgacatc     360

cagatcctgg gtatcggtca gaacggccat atcggcttca acgaaccggg caccccgctg     420

gactctctga cccacgttgt tgaactgacc gaatctacca tcaacgctaa caaacgttac     480

ttcgacaaag ttgaagacgt tccgacccgt gctgtttcta tgggtatcgg ttctatcatg     540

aaaggtaaaa aaatgatcct gatggcttac ggtgaagcta aagctgaagc tatcaaaggt     600

atgatcgacg gtccggttac caccgacatg ccggcttctg ctctgcagaa ccaccaggac     660

gttgttgtta tcatcgacga cgctgctgct tctaaactgt aa                        702


<210> 35
<211> 233
<212> PRT
<213> Artificial Sequence

<220>
<223> Ef-Thr144Ala


<400> 35
Met Gln Ile Ile Arg Val Ala Asn Ala Glu Glu Gly Gly Lys Lys Ala 
1               5                   10                  15      
Phe Glu Leu Ile Lys Glu Gly Met Asn Asn Gly Ala Lys Val Leu Gly 
            20                  25                  30          
Leu Ala Thr Gly Ser Thr Pro Glu Thr Leu Tyr Lys Glu Met Thr Ala 
        35                  40                  45              
Ser Asp Ile Asp Phe Thr Glu Met Thr Ser Val Asn Leu Asp Glu Tyr 
    50                  55                  60                  
Val Gly Leu Gly Gly Glu Asp Glu Gln Ser Tyr Arg Tyr Phe Met Asn 
65                  70                  75                  80  
Lys His Leu Phe Asp Lys Lys Pro Phe Lys Glu Thr Phe Val Pro Asn 
                85                  90                  95      
Gly Lys Ala Glu Asp Leu Asp Ala Ala Ser Ala Glu Tyr Glu Lys Ile 
            100                 105                 110         
Ile Asp Ala His Pro Val Asp Ile Gln Ile Leu Gly Ile Gly Gln Asn 
        115                 120                 125             
Gly His Ile Gly Phe Asn Glu Pro Gly Thr Pro Leu Asp Ser Leu Thr 
    130                 135                 140                 
His Val Val Glu Leu Thr Glu Ser Thr Ile Asn Ala Asn Lys Arg Tyr 
145                 150                 155                 160 
Phe Asp Lys Val Glu Asp Val Pro Thr Arg Ala Val Ser Met Gly Ile 
                165                 170                 175     
Gly Ser Ile Met Lys Gly Lys Lys Met Ile Leu Met Ala Tyr Gly Glu 
            180                 185                 190         
Ala Lys Ala Glu Ala Ile Lys Gly Met Ile Asp Gly Pro Val Thr Thr 
        195                 200                 205             
Asp Met Pro Ala Ser Ala Leu Gln Asn His Gln Asp Val Val Val Ile 
    210                 215                 220                 
Ile Asp Asp Ala Ala Ala Ser Lys Leu 
225                 230             

<210> 36
<211> 702
<212> DNA
<213> Artificial Sequence

<220>
<223> Ef-Thr144Ala


<400> 36
atgcagatca tccgtgttgc taacgctgaa gaaggtggta aaaaagcttt cgaactgatc      60

aaagaaggta tgaacaacgg tgctaaagtt ctgggtctgg ctaccggttc taccccggaa     120

accctgtaca aagaaatgac cgcttctgac atcgacttca ccgaaatgac ctctgttaac     180

ctggacgaat acgttggtct gggtggtgaa gacgaacagt cttaccgtta cttcatgaac     240

aaacacctgt tcgacaaaaa accgttcaaa gaaaccttcg ttccgaacgg taaagctgaa     300

gacctggacg ctgcttctgc tgaatacgaa aaaatcatcg acgctcaccc ggttgacatc     360

cagatcctgg gtatcggtca gaacggccat atcggcttca acgaaccggg caccccgctg     420

gactctctga cccacgttgt tgaactgacc gaatctacca tcaacgctaa caaacgttac     480

ttcgacaaag ttgaagacgt tccgacccgt gctgtttcta tgggtatcgg ttctatcatg     540

aaaggtaaaa aaatgatcct gatggcttac ggtgaagcta aagctgaagc tatcaaaggt     600

atgatcgacg gtccggttac caccgacatg ccggcttctg ctctgcagaa ccaccaggac     660

gttgttgtta tcatcgacga cgctgctgct tctaaactgt aa                        702


