                               SEQUENCE LISTING

<110> GENENTECH, INC.
 
<120> IN VITRO CELL-BASED ASSAY FOR PREDICTING PHARMACOKINETICS AND 
      BRAIN PENETRATION OF BIOLOGICS

<130> P36572-WO

<140>
<141>

<150> 63/187,750
<151> 2021-05-12

<150> 63/174,913
<151> 2021-04-14

<160> 8     

<170> PatentIn version 3.5

<210> 1
<211> 342
<212> PRT
<213> Homo sapiens

<400> 1
Ala Glu Ser His Leu Ser Leu Leu Tyr His Leu Thr Ala Val Ser Ser 
1               5                   10                  15      


Pro Ala Pro Gly Thr Pro Ala Phe Trp Val Ser Gly Trp Leu Gly Pro 
            20                  25                  30          


Gln Gln Tyr Leu Ser Tyr Asn Ser Leu Arg Gly Glu Ala Glu Pro Cys 
        35                  40                  45              


Gly Ala Trp Val Trp Glu Asn Gln Val Ser Trp Tyr Trp Glu Lys Glu 
    50                  55                  60                  


Thr Thr Asp Leu Arg Ile Lys Glu Lys Leu Phe Leu Glu Ala Phe Lys 
65                  70                  75                  80  


Ala Leu Gly Gly Lys Gly Pro Tyr Thr Leu Gln Gly Leu Leu Gly Cys 
                85                  90                  95      


Glu Leu Gly Pro Asp Asn Thr Ser Val Pro Thr Ala Lys Phe Ala Leu 
            100                 105                 110         


Asn Gly Glu Glu Phe Met Asn Phe Asp Leu Lys Gln Gly Thr Trp Gly 
        115                 120                 125             


Gly Asp Trp Pro Glu Ala Leu Ala Ile Ser Gln Arg Trp Gln Gln Gln 
    130                 135                 140                 


Asp Lys Ala Ala Asn Lys Glu Leu Thr Phe Leu Leu Phe Ser Cys Pro 
145                 150                 155                 160 


His Arg Leu Arg Glu His Leu Glu Arg Gly Arg Gly Asn Leu Glu Trp 
                165                 170                 175     


Lys Glu Pro Pro Ser Met Arg Leu Lys Ala Arg Pro Ser Ser Pro Gly 
            180                 185                 190         


Phe Ser Val Leu Thr Cys Ser Ala Phe Ser Phe Tyr Pro Pro Glu Leu 
        195                 200                 205             


Gln Leu Arg Phe Leu Arg Asn Gly Leu Ala Ala Gly Thr Gly Gln Gly 
    210                 215                 220                 


Asp Phe Gly Pro Asn Ser Asp Gly Ser Phe His Ala Ser Ser Ser Leu 
225                 230                 235                 240 


Thr Val Lys Ser Gly Asp Glu His His Tyr Cys Cys Ile Val Gln His 
                245                 250                 255     


Ala Gly Leu Ala Gln Pro Leu Arg Val Glu Leu Glu Ser Pro Ala Lys 
            260                 265                 270         


Ser Ser Val Leu Val Val Gly Ile Val Ile Gly Val Leu Leu Leu Thr 
        275                 280                 285             


Ala Ala Ala Val Gly Gly Ala Leu Leu Trp Arg Arg Met Arg Ser Gly 
    290                 295                 300                 


Leu Pro Ala Pro Trp Ile Ser Leu Arg Gly Asp Asp Thr Gly Val Leu 
305                 310                 315                 320 


Leu Pro Thr Pro Gly Glu Ala Gln Asp Ala Asp Leu Lys Asp Val Asn 
                325                 330                 335     


Val Ile Pro Ala Thr Ala 
            340         


<210> 2
<211> 119
<212> PRT
<213> Homo sapiens

<400> 2
Met Ser Arg Ser Val Ala Leu Ala Val Leu Ala Leu Leu Ser Leu Ser 
1               5                   10                  15      


Gly Leu Glu Ala Ile Gln Arg Thr Pro Lys Ile Gln Val Tyr Ser Arg 
            20                  25                  30          


His Pro Ala Glu Asn Gly Lys Ser Asn Phe Leu Asn Cys Tyr Val Ser 
        35                  40                  45              


Gly Phe His Pro Ser Asp Ile Glu Val Asp Leu Leu Lys Asn Gly Glu 
    50                  55                  60                  


Arg Ile Glu Lys Val Glu His Ser Asp Leu Ser Phe Ser Lys Asp Trp 
65                  70                  75                  80  


Ser Phe Tyr Leu Leu Tyr Tyr Thr Glu Phe Thr Pro Thr Glu Lys Asp 
                85                  90                  95      


Glu Tyr Ala Cys Arg Val Asn His Val Thr Leu Ser Gln Pro Lys Ile 
            100                 105                 110         


Val Lys Trp Asp Arg Asp Met 
        115                 


<210> 3
<211> 344
<212> PRT
<213> Mus sp.

<400> 3
Ser Glu Thr Arg Pro Pro Leu Met Tyr His Leu Thr Ala Val Ser Asn 
1               5                   10                  15      


Pro Ser Thr Gly Leu Pro Ser Phe Trp Ala Thr Gly Trp Leu Gly Pro 
            20                  25                  30          


Gln Gln Tyr Leu Thr Tyr Asn Ser Leu Arg Gln Glu Ala Asp Pro Cys 
        35                  40                  45              


Gly Ala Trp Met Trp Glu Asn Gln Val Ser Trp Tyr Trp Glu Lys Glu 
    50                  55                  60                  


Thr Thr Asp Leu Lys Ser Lys Glu Gln Leu Phe Leu Glu Ala Leu Lys 
65                  70                  75                  80  


Thr Leu Glu Lys Ile Leu Asn Gly Thr Tyr Thr Leu Gln Gly Leu Leu 
                85                  90                  95      


Gly Cys Glu Leu Ala Ser Asp Asn Ser Ser Val Pro Thr Ala Val Phe 
            100                 105                 110         


Ala Leu Asn Gly Glu Glu Phe Met Lys Phe Asn Pro Arg Ile Gly Asn 
        115                 120                 125             


Trp Thr Gly Glu Trp Pro Glu Thr Glu Ile Val Ala Asn Leu Trp Met 
    130                 135                 140                 


Lys Gln Pro Asp Ala Ala Arg Lys Glu Ser Glu Phe Leu Leu Asn Ser 
145                 150                 155                 160 


Cys Pro Glu Arg Leu Leu Gly His Leu Glu Arg Gly Arg Arg Asn Leu 
                165                 170                 175     


Glu Trp Lys Glu Pro Pro Ser Met Arg Leu Lys Ala Arg Pro Gly Asn 
            180                 185                 190         


Ser Gly Ser Ser Val Leu Thr Cys Ala Ala Phe Ser Phe Tyr Pro Pro 
        195                 200                 205             


Glu Leu Lys Phe Arg Phe Leu Arg Asn Gly Leu Ala Ser Gly Ser Gly 
    210                 215                 220                 


Asn Cys Ser Thr Gly Pro Asn Gly Asp Gly Ser Phe His Ala Trp Ser 
225                 230                 235                 240 


Leu Leu Glu Val Lys Arg Gly Asp Glu His His Tyr Gln Cys Gln Val 
                245                 250                 255     


Glu His Glu Gly Leu Ala Gln Pro Leu Thr Val Asp Leu Asp Ser Ser 
            260                 265                 270         


Ala Arg Ser Ser Val Pro Val Val Gly Ile Val Leu Gly Leu Leu Leu 
        275                 280                 285             


Val Val Val Ala Ile Ala Gly Gly Val Leu Leu Trp Gly Arg Met Arg 
    290                 295                 300                 


Ser Gly Leu Pro Ala Pro Trp Leu Ser Leu Ser Gly Asp Asp Ser Gly 
305                 310                 315                 320 


Asp Leu Leu Pro Gly Gly Asn Leu Pro Pro Glu Ala Glu Pro Gln Gly 
                325                 330                 335     


Ala Asn Ala Phe Pro Ala Thr Ser 
            340                 


<210> 4
<211> 119
<212> PRT
<213> Mus sp.

<400> 4
Met Ala Arg Ser Val Thr Leu Val Phe Leu Val Leu Val Ser Leu Thr 
1               5                   10                  15      


Gly Leu Tyr Ala Ile Gln Lys Thr Pro Gln Ile Gln Val Tyr Ser Arg 
            20                  25                  30          


His Pro Pro Glu Asn Gly Lys Pro Asn Ile Leu Asn Cys Tyr Val Thr 
        35                  40                  45              


Gln Phe His Pro Pro His Ile Glu Ile Gln Met Leu Lys Asn Gly Lys 
    50                  55                  60                  


Lys Ile Pro Lys Val Glu Met Ser Asp Met Ser Phe Ser Lys Asp Trp 
65                  70                  75                  80  


Ser Phe Tyr Ile Leu Ala His Thr Glu Phe Thr Pro Thr Glu Thr Asp 
                85                  90                  95      


Thr Tyr Ala Cys Arg Val Lys His Asp Ser Met Ala Glu Pro Lys Thr 
            100                 105                 110         


Val Tyr Trp Asp Arg Asp Met 
        115                 


<210> 5
<211> 344
<212> PRT
<213> Rattus sp.

<400> 5
Ala Glu Pro Arg Leu Pro Leu Met Tyr His Leu Ala Ala Val Ser Asp 
1               5                   10                  15      


Leu Ser Thr Gly Leu Pro Ser Phe Trp Ala Thr Gly Trp Leu Gly Ala 
            20                  25                  30          


Gln Gln Tyr Leu Thr Tyr Asn Asn Leu Arg Gln Glu Ala Asp Pro Cys 
        35                  40                  45              


Gly Ala Trp Ile Trp Glu Asn Gln Val Ser Trp Tyr Trp Glu Lys Glu 
    50                  55                  60                  


Thr Thr Asp Leu Lys Ser Lys Glu Gln Leu Phe Leu Glu Ala Ile Arg 
65                  70                  75                  80  


Thr Leu Glu Asn Gln Ile Asn Gly Thr Phe Thr Leu Gln Gly Leu Leu 
                85                  90                  95      


Gly Cys Glu Leu Ala Pro Asp Asn Ser Ser Leu Pro Thr Ala Val Phe 
            100                 105                 110         


Ala Leu Asn Gly Glu Glu Phe Met Arg Phe Asn Pro Arg Thr Gly Asn 
        115                 120                 125             


Trp Ser Gly Glu Trp Pro Glu Thr Asp Ile Val Gly Asn Leu Trp Met 
    130                 135                 140                 


Lys Gln Pro Glu Ala Ala Arg Lys Glu Ser Glu Phe Leu Leu Thr Ser 
145                 150                 155                 160 


Cys Pro Glu Arg Leu Leu Gly His Leu Glu Arg Gly Arg Gln Asn Leu 
                165                 170                 175     


Glu Trp Lys Glu Pro Pro Ser Met Arg Leu Lys Ala Arg Pro Gly Asn 
            180                 185                 190         


Ser Gly Ser Ser Val Leu Thr Cys Ala Ala Phe Ser Phe Tyr Pro Pro 
        195                 200                 205             


Glu Leu Lys Phe Arg Phe Leu Arg Asn Gly Leu Ala Ser Gly Ser Gly 
    210                 215                 220                 


Asn Cys Ser Thr Gly Pro Asn Gly Asp Gly Ser Phe His Ala Trp Ser 
225                 230                 235                 240 


Leu Leu Glu Val Lys Arg Gly Asp Glu His His Tyr Gln Cys Gln Val 
                245                 250                 255     


Glu His Glu Gly Leu Ala Gln Pro Leu Thr Val Asp Leu Asp Ser Pro 
            260                 265                 270         


Ala Arg Ser Ser Val Pro Val Val Gly Ile Ile Leu Gly Leu Leu Leu 
        275                 280                 285             


Val Val Val Ala Ile Ala Gly Gly Val Leu Leu Trp Asn Arg Met Arg 
    290                 295                 300                 


Ser Gly Leu Pro Ala Pro Trp Leu Ser Leu Ser Gly Asp Asp Ser Gly 
305                 310                 315                 320 


Asp Leu Leu Pro Gly Gly Asn Leu Pro Pro Glu Ala Glu Pro Gln Gly 
                325                 330                 335     


Val Asn Ala Phe Pro Ala Thr Ser 
            340                 


<210> 6
<211> 119
<212> PRT
<213> Rattus sp.

<400> 6
Met Ala Arg Ser Val Thr Val Ile Phe Leu Val Leu Val Ser Leu Ala 
1               5                   10                  15      


Val Val Leu Ala Ile Gln Lys Thr Pro Gln Ile Gln Val Tyr Ser Arg 
            20                  25                  30          


His Pro Pro Glu Asn Gly Lys Pro Asn Phe Leu Asn Cys Tyr Val Ser 
        35                  40                  45              


Gln Phe His Pro Pro Gln Ile Glu Ile Glu Leu Leu Lys Asn Gly Lys 
    50                  55                  60                  


Lys Ile Pro Asn Ile Glu Met Ser Asp Leu Ser Phe Ser Lys Asp Trp 
65                  70                  75                  80  


Ser Phe Tyr Ile Leu Ala His Thr Glu Phe Thr Pro Thr Glu Thr Asp 
                85                  90                  95      


Val Tyr Ala Cys Arg Val Lys His Val Thr Leu Lys Glu Pro Lys Thr 
            100                 105                 110         


Val Thr Trp Asp Arg Asp Met 
        115                 


<210> 7
<211> 342
<212> PRT
<213> Macaca fascicularis

<400> 7
Ala Glu Ser His Leu Ser Leu Leu Tyr His Leu Thr Ala Val Ser Ser 
1               5                   10                  15      


Pro Ala Pro Gly Thr Pro Ala Phe Trp Val Ser Gly Trp Leu Gly Pro 
            20                  25                  30          


Gln Gln Tyr Leu Ser Tyr Asp Ser Leu Arg Gly Gln Ala Glu Pro Cys 
        35                  40                  45              


Gly Ala Trp Val Trp Glu Asn Gln Val Ser Trp Tyr Trp Glu Lys Glu 
    50                  55                  60                  


Thr Thr Asp Leu Arg Ile Lys Glu Lys Leu Phe Leu Glu Ala Phe Lys 
65                  70                  75                  80  


Ala Leu Gly Gly Lys Gly Pro Tyr Thr Leu Gln Gly Leu Leu Gly Cys 
                85                  90                  95      


Glu Leu Ser Pro Asp Asn Thr Ser Val Pro Thr Ala Lys Phe Ala Leu 
            100                 105                 110         


Asn Gly Glu Glu Phe Met Asn Phe Asp Leu Lys Gln Gly Thr Trp Gly 
        115                 120                 125             


Gly Asp Trp Pro Glu Ala Leu Ala Ile Ser Gln Arg Trp Gln Gln Gln 
    130                 135                 140                 


Asp Lys Ala Ala Asn Lys Glu Leu Thr Phe Leu Leu Phe Ser Cys Pro 
145                 150                 155                 160 


His Arg Leu Arg Glu His Leu Glu Arg Gly Arg Gly Asn Leu Glu Trp 
                165                 170                 175     


Lys Glu Pro Pro Ser Met Arg Leu Lys Ala Arg Pro Gly Asn Pro Gly 
            180                 185                 190         


Phe Ser Val Leu Thr Cys Ser Ala Phe Ser Phe Tyr Pro Pro Glu Leu 
        195                 200                 205             


Gln Leu Arg Phe Leu Arg Asn Gly Met Ala Ala Gly Thr Gly Gln Gly 
    210                 215                 220                 


Asp Phe Gly Pro Asn Ser Asp Gly Ser Phe His Ala Ser Ser Ser Leu 
225                 230                 235                 240 


Thr Val Lys Ser Gly Asp Glu His His Tyr Cys Cys Ile Val Gln His 
                245                 250                 255     


Ala Gly Leu Ala Gln Pro Leu Arg Val Glu Leu Glu Thr Pro Ala Lys 
            260                 265                 270         


Ser Ser Val Leu Val Val Gly Ile Val Ile Gly Val Leu Leu Leu Thr 
        275                 280                 285             


Ala Ala Ala Val Gly Gly Ala Leu Leu Trp Arg Arg Met Arg Ser Gly 
    290                 295                 300                 


Leu Pro Ala Pro Trp Ile Ser Leu Arg Gly Asp Asp Thr Gly Ser Leu 
305                 310                 315                 320 


Leu Pro Thr Pro Gly Glu Ala Gln Asp Ala Asp Ser Lys Asp Ile Asn 
                325                 330                 335     


Val Ile Pro Ala Thr Ala 
            340         


<210> 8
<211> 119
<212> PRT
<213> Macaca fascicularis

<400> 8
Met Ser Pro Ser Val Ala Leu Ala Val Leu Ala Leu Leu Ser Pro Ser 
1               5                   10                  15      


Gly Leu Glu Ala Ile Gln Arg Thr Pro Lys Ile Gln Val Tyr Ser Arg 
            20                  25                  30          


His Pro Pro Glu Asn Gly Lys Pro Asn Phe Leu Asn Cys Tyr Val Ser 
        35                  40                  45              


Gly Phe His Pro Ser Asp Ile Glu Val Asp Leu Leu Lys Asn Gly Glu 
    50                  55                  60                  


Lys Met Gly Lys Val Glu His Ser Asp Leu Ser Phe Ser Lys Asp Trp 
65                  70                  75                  80  


Ser Phe Tyr Leu Leu Tyr Tyr Thr Glu Phe Thr Pro Asn Glu Lys Asp 
                85                  90                  95      


Glu Tyr Ala Cys Arg Val Asn His Val Thr Leu Ser Gly Pro Arg Thr 
            100                 105                 110         


Val Lys Trp Asp Arg Asp Met 
        115                 


