<110>    CJ CheilJedang Corporation

<120>    Novel sugar phosphate isomerase/epimerase variant and a method
         for producing L-lysine using the same

<130>    OPP20212330KR

<150>    KR 10-2021-0047419
<151>    2021-04-12

<160>    10

<170>    KoPatentIn 3.0

<210>    1
<211>    337
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    (A321V)_sugar phosphate isomerase/epimerase


<400>    1
Met Lys Leu Gly Leu Tyr Asn Ala Ile Phe His Asp Arg Thr Leu Pro
  1               5                  10                  15 

Glu Ala Leu Ala Ala Ile Lys Ala Ala Gly Leu Thr Gly Ile Glu Leu
             20                  25                  30 

Asn Thr Gly Gly Phe Leu Pro Ala Thr His Ile Pro Thr Ile Asp Asp
         35                  40                  45 

Ile Leu Val Ser Asp Asp Ala Arg Asp Glu Phe Leu Gly Ile Phe Glu
     50                  55                  60 

Gly Thr Gly Val Asp Ile Tyr Gly Leu Asn Cys Asn Gly Asn Pro Leu
 65                  70                  75                  80 

His Pro Asn Lys Ala Ile Gly Asp Lys His Ala Glu Asp Ile Arg Arg
                 85                  90                  95 

Ser Ile Arg Leu Ala Glu Arg Leu Gly Gln Asn Arg Val Val Thr Met
            100                 105                 110 

Ser Gly Leu Pro Gly Gly Glu Pro Gly Ala Lys Tyr Thr Asn Trp Val
        115                 120                 125 

Val Asn Ala Trp Asn Ser Ala Ala Leu Asp Val Leu Asp Tyr Gln Trp
    130                 135                 140 

Asp Ile Ala Ala Glu Phe Trp Arg Glu Thr Asp Arg Phe Ala Ala Asp
145                 150                 155                 160 

His Gly Val Lys Val Ala Leu Glu Leu His Pro Gln Asn Ile Val Phe
                165                 170                 175 

Asn Ser Ala Asp Val His Lys Leu Ile Asp Leu Thr Gly Ala Thr His
            180                 185                 190 

Val Gly Val Glu Leu Asp Ala Ser His Leu Phe Trp Gln Gln Met Asp
        195                 200                 205 

Pro Ile Ala Val Ile Asp His Leu Gly Glu Leu Ile Phe His Ala Ala
    210                 215                 220 

Ala Lys Asp Val Arg Val Asn Lys Glu Trp Ala Gln Leu Asn Gly Val
225                 230                 235                 240 

Leu Asp Asn Ser Phe Arg Arg Leu Asp Pro Ser Glu Asn Arg Thr Asn
                245                 250                 255 

Leu Gly Gly Asp Glu Trp Ala Asn Glu Trp Pro Lys Asn Ser Ala Trp
            260                 265                 270 

Asp Phe Val Ala Leu Gly Arg Gly His Asp Val Ala Tyr Trp Thr Glu
        275                 280                 285 

Phe Leu Arg Ala Leu His Arg Val Asp Pro Asn Met Leu Val Asn Ile
    290                 295                 300 

Glu His Glu Asp Val Ser Leu Gly Arg Glu Glu Gly Val Asn Glu Ala
305                 310                 315                 320 

Val Lys Val Leu Ile Glu Ala Asn Lys Ala Leu Glu Glu Ser Leu Val
                325                 330                 335 

Ser
   


<210>    2
<211>    1014
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    (A321V)_NCgl0169


<400>    2
atgaaactcg gtctctacaa cgcgatcttc cacgaccgca ccctgccaga agcgctcgca         60

gccatcaaag ctgcaggtct caccggaatt gaactcaaca ccggcggatt tttgcctgca        120

acccacatcc cgaccatcga tgacatcctg gtcagcgatg atgcccgcga tgaattcctc        180

gggattttcg aaggcaccgg cgtcgacatc tacggcctta actgcaacgg caacccgctt        240

caccccaaca aggcgatcgg ggacaagcat gccgaagaca ttcgacgttc catccgcctc        300

gcagagcgcc tcggccaaaa ccgtgtggtc accatgtctg gtcttcctgg tggcgaacca        360

ggcgcgaagt acaccaactg ggttgtcaac gcgtggaact ccgcagcctt ggatgtcctt        420

gattaccaat gggatatcgc agctgaattc tggcgcgaga ccgaccgctt cgccgcagat        480

cacggcgtga aagtggctct tgagctgcac ccacagaaca tcgtgttcaa ctccgctgac        540

gtgcataagc tcatcgatct caccggcgcc acccacgtgg gcgtcgaact ggatgcatca        600

cacctgttct ggcagcagat ggacccaatc gctgtgattg atcacctcgg cgagctcatc        660

ttccacgccg ccgccaaaga cgtgcgagtt aataaggaat gggctcagct caacggtgtg        720

ctggacaaca gcttccgacg ccttgaccca tccgaaaacc gcaccaactt gggcggcgac        780

gagtgggcga atgaatggcc aaagaactct gcttgggatt tcgttgctct gggccgcggt        840

catgacgttg cttactggac cgaattcctc cgcgcacttc accgcgtcga tccaaacatg        900

ctggtcaaca tcgaacacga ggatgtttca ctcggtcgcg aagaaggcgt caacgaagcc        960

gttaaggtgc tgatcgaggc caacaaggca ctcgaagagt ccctggtttc ttaa             1014


<210>    3
<211>    337
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    (WT)_sugar phosphate isomerase/epimerase


<400>    3
Met Lys Leu Gly Leu Tyr Asn Ala Ile Phe His Asp Arg Thr Leu Pro
  1               5                  10                  15 

Glu Ala Leu Ala Ala Ile Lys Ala Ala Gly Leu Thr Gly Ile Glu Leu
             20                  25                  30 

Asn Thr Gly Gly Phe Leu Pro Ala Thr His Ile Pro Thr Ile Asp Asp
         35                  40                  45 

Ile Leu Val Ser Asp Asp Ala Arg Asp Glu Phe Leu Gly Ile Phe Glu
     50                  55                  60 

Gly Thr Gly Val Asp Ile Tyr Gly Leu Asn Cys Asn Gly Asn Pro Leu
 65                  70                  75                  80 

His Pro Asn Lys Ala Ile Gly Asp Lys His Ala Glu Asp Ile Arg Arg
                 85                  90                  95 

Ser Ile Arg Leu Ala Glu Arg Leu Gly Gln Asn Arg Val Val Thr Met
            100                 105                 110 

Ser Gly Leu Pro Gly Gly Glu Pro Gly Ala Lys Tyr Thr Asn Trp Val
        115                 120                 125 

Val Asn Ala Trp Asn Ser Ala Ala Leu Asp Val Leu Asp Tyr Gln Trp
    130                 135                 140 

Asp Ile Ala Ala Glu Phe Trp Arg Glu Thr Asp Arg Phe Ala Ala Asp
145                 150                 155                 160 

His Gly Val Lys Val Ala Leu Glu Leu His Pro Gln Asn Ile Val Phe
                165                 170                 175 

Asn Ser Ala Asp Val His Lys Leu Ile Asp Leu Thr Gly Ala Thr His
            180                 185                 190 

Val Gly Val Glu Leu Asp Ala Ser His Leu Phe Trp Gln Gln Met Asp
        195                 200                 205 

Pro Ile Ala Val Ile Asp His Leu Gly Glu Leu Ile Phe His Ala Ala
    210                 215                 220 

Ala Lys Asp Val Arg Val Asn Lys Glu Trp Ala Gln Leu Asn Gly Val
225                 230                 235                 240 

Leu Asp Asn Ser Phe Arg Arg Leu Asp Pro Ser Glu Asn Arg Thr Asn
                245                 250                 255 

Leu Gly Gly Asp Glu Trp Ala Asn Glu Trp Pro Lys Asn Ser Ala Trp
            260                 265                 270 

Asp Phe Val Ala Leu Gly Arg Gly His Asp Val Ala Tyr Trp Thr Glu
        275                 280                 285 

Phe Leu Arg Ala Leu His Arg Val Asp Pro Asn Met Leu Val Asn Ile
    290                 295                 300 

Glu His Glu Asp Val Ser Leu Gly Arg Glu Glu Gly Val Asn Glu Ala
305                 310                 315                 320 

Ala Lys Val Leu Ile Glu Ala Asn Lys Ala Leu Glu Glu Ser Leu Val
                325                 330                 335 

Ser
   


<210>    4
<211>    1014
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    (WT)_NCgl0169


<400>    4
atgaaactcg gtctctacaa cgcgatcttc cacgaccgca ccctgccaga agcgctcgca         60

gccatcaaag ctgcaggtct caccggaatt gaactcaaca ccggcggatt tttgcctgca        120

acccacatcc cgaccatcga tgacatcctg gtcagcgatg atgcccgcga tgaattcctc        180

gggattttcg aaggcaccgg cgtcgacatc tacggcctta actgcaacgg caacccgctt        240

caccccaaca aggcgatcgg ggacaagcat gccgaagaca ttcgacgttc catccgcctc        300

gcagagcgcc tcggccaaaa ccgtgtggtc accatgtctg gtcttcctgg tggcgaacca        360

ggcgcgaagt acaccaactg ggttgtcaac gcgtggaact ccgcagcctt ggatgtcctt        420

gattaccaat gggatatcgc agctgaattc tggcgcgaga ccgaccgctt cgccgcagat        480

cacggcgtga aagtggctct tgagctgcac ccacagaaca tcgtgttcaa ctccgctgac        540

gtgcataagc tcatcgatct caccggcgcc acccacgtgg gcgtcgaact ggatgcatca        600

cacctgttct ggcagcagat ggacccaatc gctgtgattg atcacctcgg cgagctcatc        660

ttccacgccg ccgccaaaga cgtgcgagtt aataaggaat gggctcagct caacggtgtg        720

ctggacaaca gcttccgacg ccttgaccca tccgaaaacc gcaccaactt gggcggcgac        780

gagtgggcga atgaatggcc aaagaactct gcttgggatt tcgttgctct gggccgcggt        840

catgacgttg cttactggac cgaattcctc cgcgcacttc accgcgtcga tccaaacatg        900

ctggtcaaca tcgaacacga ggatgtttca ctcggtcgcg aagaaggcgt caacgaagcc        960

gctaaggtgc tgatcgaggc caacaaggca ctcgaagagt ccctggtttc ttaa             1014


<210>    5
<211>    37
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    NCgl0169_1F


<400>    5
tgaattcgag ctcggtaccc gcttcgccgc agatcac                                  37


<210>    6
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    NCgl0169_2R


<400>    6
ctcgatcagc accttaacgg cttcgttgac gcc                                      33


<210>    7
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    NCgl0169_3F


<400>    7
ggcgtcaacg aagccgttaa ggtgctgatc gag                                      33


<210>    8
<211>    38
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    NCgl0169_4R


<400>    8
gtcgactcta gaggatcccc gcgctcttcg tcaagtac                                 38


<210>    9
<211>    17
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    NCgl0169_5F


<400>    9
ccaggcgcga agtacac                                                        17


<210>    10
<211>    17
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    NCgl0169_6R


<400>    10
cgacggggag tgaccac                                                        17
