               SEQUENCE LISTING

<110> INSERM (Institut National de la Santé et de la Recherche Médicale)
      OGD2 PHARMA
      Nantes Université

<120> NEW METHOD TO IMPROVE THE ANTI-TUMORAL ACTIVITY OF
MACROPHAGES

<130> BIO19527 BIRKLE

<150> EP21305491.9
<151> 2021-04-14

<160> 38

<170> BiSSAP 1.3.6

<210> 1
<211> 8
<212> PRT
<213> Artificial Sequence


<220> 
<223> 8B6 VH-CDR1

<400> 1
Glu Phe Thr Phe Thr Asp Tyr Tyr 
1               5               

<210> 2
<211> 10
<212> PRT
<213> Artificial Sequence


<220> 
<223> 8B6 VH-CDR2

<400> 2
Ile Arg Asn Arg Ala Asn Gly Tyr Thr Thr 
1               5                   10  

<210> 3
<211> 10
<212> PRT
<213> Artificial Sequence


<220> 
<223> 8B6 VH-CDR3

<400> 3
Ala Arg Val Ser Asn Trp Ala Phe Asp Tyr 
1               5                   10  

<210> 4
<211> 12
<212> PRT
<213> Artificial Sequence


<220> 
<223> 8B6 VL-CDR1

<400> 4
Gln Ser Leu Leu Lys Asn Asn Gly Asn Thr Phe Leu 
1               5                   10          

<210> 5
<211> 3
<212> PRT
<213> Artificial Sequence


<220> 
<223> 8B6 VL-CDR2

<400> 5
Lys Val Ser 
1           

<210> 6
<211> 9
<212> PRT
<213> Artificial Sequence


<220> 
<223> 8B6 VL-CDR3

<400> 6
Ser Gln Ser Thr His Ile Pro Tyr Thr 
1               5                   

<210> 7
<211> 112
<212> PRT
<213> Artificial Sequence


<220> 
<221> SITE
<222> 1..1
<223> X=D or E

<220> 
<223> Humanized anti-OAcGD2 VL consensus sequence

<220> 
<221> SITE
<222> 2..2
<223> X=V or I

<220> 
<221> SITE
<222> 9..9
<223> X=L or A

<220> 
<221> SITE
<222> 10..10
<223> X=S or T

<220> 
<221> SITE
<222> 12..12
<223> X=P or S

<220> 
<221> SITE
<222> 13..13
<223> X=V or L or A

<220> 
<221> SITE
<222> 14..14
<223> X=S or T

<220> 
<221> SITE
<222> 15..15
<223> X=L or P or V

<220> 
<221> SITE
<222> 17..17
<223> X=D or Q or E

<220> 
<221> SITE
<222> 18..18
<223> X=Q or P or R

<220> 
<221> SITE
<222> 19..19
<223> X=A or V

<220> 
<221> SITE
<222> 20..20
<223> X=S or T

<220> 
<221> SITE
<222> 21..21
<223> X=I or L

<220> 
<221> SITE
<222> 22..22
<223> X=S or T

<220> 
<221> SITE
<222> 25..25
<223> X=S or A

<220> 
<221> SITE
<222> 29..29
<223> X=L or V

<220> 
<221> SITE
<222> 30..30
<223> X=L or V

<220> 
<221> SITE
<222> 32..32
<223> X=N or S

<220> 
<221> SITE
<222> 33..33
<223> X=N or Q

<220> 
<221> SITE
<222> 34..34
<223> X=G or A or S

<220> 
<221> SITE
<222> 35..35
<223> X=N or Y or S

<220> 
<221> SITE
<222> 36..36
<223> X=T or N or S

<220> 
<221> SITE
<222> 37..37
<223> X=F or Y

<220> 
<221> SITE
<222> 39..39
<223> X=H or N or S or A or Y or G

<220> 
<221> SITE
<222> 41..41
<223> X=Y or F

<220> 
<221> SITE
<222> 42..42
<223> X=L or Q

<220> 
<221> SITE
<222> 44..44
<223> X=K or R

<220> 
<221> SITE
<222> 47..47
<223> X=Q or K

<220> 
<221> SITE
<222> 48..48
<223> X=S or A or V

<220> 
<221> SITE
<222> 50..50
<223> X=K or Q or R

<220> 
<221> SITE
<222> 51..51
<223> X=L or R or V

<220> 
<221> SITE
<222> 55..55
<223> X=K or G or L

<220> 
<221> SITE
<222> 56..56
<223> X=V or A or G

<220> 
<221> SITE
<222> 58..58
<223> X=N or T

<220> 
<221> SITE
<222> 60..60
<223> X=L or D or A

<220> 
<221> SITE
<222> 61..61
<223> X=S or T

<220> 
<221> SITE
<222> 63..63
<223> X=V or I

<220> 
<221> SITE
<222> 65..65
<223> X=D or A or S

<220> 
<221> SITE
<222> 75..75
<223> X=Y or D

<220> 
<221> SITE
<222> 79..79
<223> X=K or T

<220> 
<221> SITE
<222> 81..81
<223> X=S or N

<220> 
<221> SITE
<222> 82..82
<223> X=R or S

<220> 
<221> SITE
<222> 83..83
<223> X=V or L

<220> 
<221> SITE
<222> 84..84
<223> X=E or Q

<220> 
<221> SITE
<222> 85..85
<223> X=A or P

<220> 
<221> SITE
<222> 88..88
<223> X=L or V or F

<220> 
<221> SITE
<222> 89..89
<223> X=G or A

<220> 
<221> SITE
<222> 90..90
<223> X=V or T

<220> 
<221> SITE
<222> 92..92
<223> X=F or Y

<220> 
<221> SITE
<222> 94..94
<223> X=S or M or Q

<220> 
<221> SITE
<222> 96..96
<223> X=S or A

<220> 
<221> SITE
<222> 97..97
<223> X=T or Y

<220> 
<221> SITE
<222> 98..98
<223> X=H or Q or N

<220> 
<221> SITE
<222> 99..99
<223> X=I or T or Q

<220> 
<221> SITE
<222> 100..100
<223> X=P or S

<220> 
<221> SITE
<222> 105..105
<223> X=G or Q

<400> 7
Xaa Xaa Val Met Thr Gln Ser Pro Xaa Xaa Leu Xaa Xaa Xaa Xaa Gly 
1               5                   10                  15      
Xaa Xaa Xaa Xaa Xaa Xaa Cys Arg Xaa Ser Gln Ser Xaa Xaa Lys Xaa 
            20                  25                  30          
Xaa Xaa Xaa Xaa Xaa Leu Xaa Trp Xaa Xaa Gln Xaa Pro Gly Xaa Xaa 
        35                  40                  45              
Pro Xaa Xaa Leu Ile Tyr Xaa Xaa Ser Xaa Arg Xaa Xaa Gly Xaa Pro 
    50                  55                  60                  
Xaa Arg Phe Ser Gly Ser Gly Ser Gly Thr Xaa Phe Thr Leu Xaa Ile 
65                  70                  75                  80  
Xaa Xaa Xaa Xaa Xaa Glu Asp Xaa Xaa Xaa Tyr Xaa Cys Xaa Gln Xaa 
                85                  90                  95      
Xaa Xaa Xaa Xaa Tyr Thr Phe Gly Xaa Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110         



<210> 8
<211> 119
<212> PRT
<213> Artificial Sequence


<220> 
<221> SITE
<222> 1..1
<223> X=E or Q

<220> 
<223> Humanized anti-OAcGD2 VH consensus sequence

<220> 
<221> SITE
<222> 5..5
<223> X=V or L

<220> 
<221> SITE
<222> 13..13
<223> X=Q or K

<220> 
<221> SITE
<222> 16..16
<223> X=G or R

<220> 
<221> SITE
<222> 23..23
<223> X=A or T

<220> 
<221> SITE
<222> 24..24
<223> X=T or A

<220> 
<221> SITE
<222> 26..26
<223> X=E or G

<220> 
<221> SITE
<222> 30..30
<223> X=T or S or G

<220> 
<221> SITE
<222> 32..32
<223> X=Y or H

<220> 
<221> SITE
<222> 35..35
<223> X=T or H or N or S

<220> 
<221> SITE
<222> 37..37
<223> X=V or I

<220> 
<221> SITE
<222> 48..48
<223> X= L or V

<220> 
<221> SITE
<222> 49..49
<223> X= G or S

<220> 
<221> SITE
<222> 50..50
<223> X= F or Y

<220> 
<221> SITE
<222> 51..51
<223> X= I or T

<220> 
<221> SITE
<222> 54..54
<223> X= R or K or S

<220> 
<221> SITE
<222> 55..55
<223> X= A or S

<220> 
<221> SITE
<222> 56..56
<223> X=N or S

<220> 
<221> SITE
<222> 57..57
<223> X= G or A or S

<220> 
<221> SITE
<222> 58..58
<223> X= Y or G

<220> 
<221> SITE
<222> 59..59
<223> X= T or I

<220> 
<221> SITE
<222> 60..60
<223> X= T or I

<220> 
<221> SITE
<222> 61..61
<223> X= E or Y

<220> 
<221> SITE
<222> 63..63
<223> X= N or A

<220> 
<221> SITE
<222> 64..64
<223> X= P or A or D

<220> 
<221> SITE
<222> 76..76
<223> X= N or G

<220> 
<221> SITE
<222> 77..77
<223> X= S or A

<220> 
<221> SITE
<222> 79..79
<223> X= S or N

<220> 
<221> SITE
<222> 80..80
<223> X= I or S or T

<220> 
<221> SITE
<222> 81..81
<223> X= L or T or A

<220> 
<221> SITE
<222> 89..89
<223> X= R or K or Q

<220> 
<221> SITE
<222> 90..90
<223> X= T or A

<220> 
<221> SITE
<222> 95..95
<223> X= V or I or L

<220> 
<221> SITE
<222> 99..99
<223> X= A or T

<220> 
<221> SITE
<222> 105..105
<223> X=A or Y

<220> 
<221> SITE
<222> 114..114
<223> X= T or L

<220> 
<221> SITE
<222> 115..115
<223> X= L or V

<400> 8
Xaa Val Gln Leu Xaa Glu Ser Gly Gly Gly Leu Val Xaa Pro Gly Xaa 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Xaa Xaa Ser Xaa Phe Thr Phe Xaa Asp Xaa 
            20                  25                  30          
Tyr Met Xaa Trp Xaa Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Xaa 
        35                  40                  45              
Xaa Xaa Xaa Arg Asn Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Tyr Xaa Xaa 
    50                  55                  60                  
Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Xaa Xaa Lys Xaa Xaa 
65                  70                  75                  80  
Xaa Tyr Leu Gln Met Asn Ser Leu Xaa Xaa Glu Asp Thr Ala Xaa Tyr 
                85                  90                  95      
Tyr Cys Xaa Arg Val Ser Asn Trp Xaa Phe Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         
Thr Xaa Xaa Thr Val Ser Ser 
        115                 

<210> 9
<211> 119
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-OAcGD2 VH49A

<400> 9
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Arg 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Thr Thr Ser Glu Phe Thr Phe Thr Asp Tyr 
            20                  25                  30          
Tyr Met Thr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              
Gly Phe Ile Arg Asn Arg Ala Asn Gly Tyr Thr Thr Glu Tyr Asn Pro 
    50                  55                  60                  
Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Ser Ile 
65                  70                  75                  80  
Leu Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Val Tyr 
                85                  90                  95      
Tyr Cys Ala Arg Val Ser Asn Trp Ala Phe Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         
Thr Leu Val Thr Val Ser Ser 
        115                 

<210> 10
<211> 119
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-OAcGD2 VH72A

<400> 10
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Ala Thr Ser Glu Phe Thr Phe Thr Asp Tyr 
            20                  25                  30          
Tyr Met Thr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              
Gly Phe Ile Arg Asn Arg Ala Asn Gly Tyr Thr Thr Glu Tyr Asn Pro 
    50                  55                  60                  
Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Ser 
65                  70                  75                  80  
Leu Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Val Tyr 
                85                  90                  95      
Tyr Cys Ala Arg Val Ser Asn Trp Ala Phe Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         
Thr Leu Val Thr Val Ser Ser 
        115                 

<210> 11
<211> 119
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-OAcGD2 VH49BHS

<400> 11
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Arg 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Thr Thr Ser Glu Phe Thr Phe Thr Asp Tyr 
            20                  25                  30          
Tyr Met Thr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              
Gly Phe Ile Arg Asn Lys Ala Asn Gly Tyr Thr Thr Glu Tyr Asn Pro 
    50                  55                  60                  
Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Ser Ile 
65                  70                  75                  80  
Leu Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Val Tyr 
                85                  90                  95      
Tyr Cys Ala Arg Val Ser Asn Trp Ala Phe Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         
Thr Leu Val Thr Val Ser Ser 
        115                 

<210> 12
<211> 119
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-OAcGD2 VH72BHNPS

<400> 12
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Ala Thr Ser Glu Phe Thr Phe Ser Asp Tyr 
            20                  25                  30          
Tyr Met Thr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              
Gly Phe Ile Arg Asn Lys Ala Asn Gly Tyr Thr Thr Glu Tyr Asn Pro 
    50                  55                  60                  
Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Ser 
65                  70                  75                  80  
Leu Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Val Tyr 
                85                  90                  95      
Tyr Cys Ala Arg Val Ser Asn Trp Ala Phe Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         
Thr Leu Val Thr Val Ser Ser 
        115                 

<210> 13
<211> 112
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-OAcGD2 VL30A

<400> 13
Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Leu Gly 
1               5                   10                  15      
Gln Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Leu Lys Asn 
            20                  25                  30          
Asn Gly Asn Thr Phe Leu His Trp Tyr Gln Gln Arg Pro Gly Gln Ser 
        35                  40                  45              
Pro Arg Leu Leu Ile Tyr Lys Val Ser Asn Arg Leu Ser Gly Val Pro 
    50                  55                  60                  
Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  
Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Phe Cys Ser Gln Ser 
                85                  90                  95      
Thr His Ile Pro Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110         



<210> 14
<211> 112
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-OAcGD2 VL28A

<400> 14
Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Pro Gly 
1               5                   10                  15      
Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Leu Lys Asn 
            20                  25                  30          
Asn Gly Asn Thr Phe Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              
Pro Gln Leu Leu Ile Tyr Lys Val Ser Asn Arg Leu Ser Gly Val Pro 
    50                  55                  60                  
Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  
Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Phe Cys Ser Gln Ser 
                85                  90                  95      
Thr His Ile Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110         



<210> 15
<211> 112
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-OAcGD2 VL28Bs01/A2

<400> 15
Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Pro Gly 
1               5                   10                  15      
Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Leu Lys Ser 
            20                  25                  30          
Asn Ala Asn Thr Phe Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              
Pro Gln Leu Leu Ile Tyr Lys Val Ser Asn Arg Leu Ser Gly Val Pro 
    50                  55                  60                  
Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  
Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gln Ser 
                85                  90                  95      
Thr His Ile Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110         



<210> 16
<211> 119
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-OAcGD2 VH49BHSs

<400> 16
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Arg 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Thr Thr Ser Glu Phe Thr Phe Thr Asp Tyr 
            20                  25                  30          
Tyr Met Thr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              
Gly Phe Ile Arg Asn Lys Ala Ser Gly Tyr Thr Thr Glu Tyr Asn Pro 
    50                  55                  60                  
Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Ser Ile 
65                  70                  75                  80  
Leu Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Val Tyr 
                85                  90                  95      
Tyr Cys Ala Arg Val Ser Asn Trp Ala Phe Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         
Thr Leu Val Thr Val Ser Ser 
        115                 

<210> 17
<211> 119
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-OAcGD2 VH72BHNPSs

<400> 17
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Ala Thr Ser Glu Phe Thr Phe Ser Asp Tyr 
            20                  25                  30          
Tyr Met Thr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              
Gly Phe Ile Arg Asn Lys Ala Asn Ser Tyr Thr Thr Glu Tyr Asn Pro 
    50                  55                  60                  
Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Ser 
65                  70                  75                  80  
Leu Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Val Tyr 
                85                  90                  95      
Tyr Cys Ala Arg Val Ser Asn Trp Ala Phe Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         
Thr Leu Val Thr Val Ser Ser 
        115                 

<210> 18
<211> 112
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-OAcGD2 VL30As

<400> 18
Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Leu Gly 
1               5                   10                  15      
Gln Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Leu Lys Asn 
            20                  25                  30          
Gln Gly Asn Thr Phe Leu His Trp Tyr Gln Gln Arg Pro Gly Gln Ser 
        35                  40                  45              
Pro Arg Leu Leu Ile Tyr Lys Val Ser Asn Arg Leu Ser Gly Val Pro 
    50                  55                  60                  
Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  
Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Phe Cys Ser Gln Ser 
                85                  90                  95      
Thr His Ile Pro Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110         



<210> 19
<211> 112
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-OAcGD2 VL28B01/A2

<400> 19
Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Pro Gly 
1               5                   10                  15      
Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Leu Lys Ser 
            20                  25                  30          
Asn Gly Asn Thr Phe Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              
Pro Gln Leu Leu Ile Tyr Lys Val Ser Asn Arg Leu Ser Gly Val Pro 
    50                  55                  60                  
Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  
Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gln Ser 
                85                  90                  95      
Thr His Ile Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110         



<210> 20
<211> 449
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-OAcGD2 HC (anti-OAcGD2 anti-CD47 bispecific)

<400> 20
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Ala Thr Ser Glu Phe Thr Phe Ser Asp Tyr 
            20                  25                  30          
Tyr Met Thr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              
Gly Phe Ile Arg Asn Lys Ala Asn Ser Tyr Thr Thr Glu Tyr Asn Pro 
    50                  55                  60                  
Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Ser 
65                  70                  75                  80  
Leu Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Val Tyr 
                85                  90                  95      
Tyr Cys Ala Arg Val Ser Asn Trp Ala Phe Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         
Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe 
        115                 120                 125             
Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu 
    130                 135                 140                 
Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp 
145                 150                 155                 160 
Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu 
                165                 170                 175     
Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser 
            180                 185                 190         
Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro 
        195                 200                 205             
Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys 
    210                 215                 220                 
Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro 
225                 230                 235                 240 
Asp Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser 
                245                 250                 255     
Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp 
            260                 265                 270         
Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn 
        275                 280                 285             
Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val 
    290                 295                 300                 
Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu 
305                 310                 315                 320 
Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Glu Glu Lys 
                325                 330                 335     
Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 
            340                 345                 350         
Leu Pro Pro Cys Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Trp 
        355                 360                 365             
Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
    370                 375                 380                 
Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
385                 390                 395                 400 
Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys 
                405                 410                 415     
Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Leu His Glu 
            420                 425                 430         
Ala Leu His Ser His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
        435                 440                 445             
Lys 
    

<210> 21
<211> 219
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-OAcGD2 LC (anti-OAcGD2 anti-CD47 bispecific)

<400> 21
Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Leu Gly 
1               5                   10                  15      
Gln Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Leu Lys Asn 
            20                  25                  30          
Asn Gly Asn Thr Phe Leu His Trp Tyr Gln Gln Arg Pro Gly Gln Ser 
        35                  40                  45              
Pro Arg Leu Leu Ile Tyr Lys Val Ser Asn Arg Leu Ser Gly Val Pro 
    50                  55                  60                  
Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  
Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Phe Cys Ser Gln Ser 
                85                  90                  95      
Thr His Ile Pro Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110         
Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
        115                 120                 125             
Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
    130                 135                 140                 
Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
145                 150                 155                 160 
Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
                165                 170                 175     
Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
            180                 185                 190         
Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
        195                 200                 205             
Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215                 

<210> 22
<211> 355
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-CD47 VHH (anti-OAcGD2 anti-CD47 bispecific)

<400> 22
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Glu Pro Gly Gly 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ile Ile Phe Lys Ile Asn 
            20                  25                  30          
Asp Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Arg Arg Glu Trp Val 
        35                  40                  45              
Ala Ala Ser Thr Gly Gly Asp Glu Ala Ile Tyr Arg Asp Ser Val Lys 
    50                  55                  60                  
Asp Arg Phe Thr Ile Ser Arg Asp Ala Lys Asn Ser Val Phe Leu Gln 
65                  70                  75                  80  
Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Thr Ala 
                85                  90                  95      
Val Ile Ser Thr Asp Arg Asp Gly Thr Glu Trp Arg Arg Tyr Trp Gly 
            100                 105                 110         
Gln Gly Thr Gln Val Thr Val Ser Ser Gly Gly Gly Gly Gly Gly Ser 
        115                 120                 125             
Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
    130                 135                 140                 
Gly Pro Asp Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
145                 150                 155                 160 
Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
                165                 170                 175     
Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
            180                 185                 190         
His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
        195                 200                 205             
Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
    210                 215                 220                 
Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Glu 
225                 230                 235                 240 
Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
                245                 250                 255     
Cys Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 
            260                 265                 270         
Leu Ser Cys Ala Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
        275                 280                 285             
Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
    290                 295                 300                 
Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Val Ser Lys Leu Thr Val 
305                 310                 315                 320 
Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Leu 
                325                 330                 335     
His Glu Ala Leu His Ser Arg Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
            340                 345                 350         
Pro Gly Lys 
        355 

<210> 23
<211> 449
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-DOTA HC (anti-DOTA anti-CD47 bispecific)

<400> 23
Glu Val Met Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly 
1               5                   10                  15      
Ser Leu Thr Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Thr Ala His 
            20                  25                  30          
Ala Met Ser Trp Val Arg Gln Thr Pro Glu Lys Arg Leu Glu Trp Val 
        35                  40                  45              
Ala Thr Ile Ser Gly Gly Gly Thr Tyr Thr Tyr Phe Pro Asp Ser Phe 
    50                  55                  60                  
Gln Gly Arg Phe Thr Ile Ser Ser Asp Asn Ala Lys Asn Thr Leu Tyr 
65                  70                  75                  80  
Leu Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ser Met Tyr Phe Cys 
                85                  90                  95      
Thr Arg His Gly Asp Tyr Arg Tyr Ala Phe Gly Tyr Trp Gly Gln Gly 
            100                 105                 110         
Thr Leu Val Thr Val Ser Ala Ala Ser Thr Lys Gly Pro Ser Val Phe 
        115                 120                 125             
Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu 
    130                 135                 140                 
Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp 
145                 150                 155                 160 
Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu 
                165                 170                 175     
Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser 
            180                 185                 190         
Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro 
        195                 200                 205             
Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys 
    210                 215                 220                 
Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro 
225                 230                 235                 240 
Asp Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser 
                245                 250                 255     
Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp 
            260                 265                 270         
Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn 
        275                 280                 285             
Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val 
    290                 295                 300                 
Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu 
305                 310                 315                 320 
Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Glu Glu Lys 
                325                 330                 335     
Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 
            340                 345                 350         
Leu Pro Pro Cys Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Trp 
        355                 360                 365             
Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
    370                 375                 380                 
Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
385                 390                 395                 400 
Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys 
                405                 410                 415     
Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Leu His Glu 
            420                 425                 430         
Ala Leu His Ser His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
        435                 440                 445             
Lys 
    

<210> 24
<211> 216
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-DOTA LC (anti-DOTA anti-CD47 bispecific)

<400> 24
Gln Ala Val Thr Gln Glu Ser Ala Leu Thr Thr Ser Pro Gly Glu Thr 
1               5                   10                  15      
Val Thr Leu Thr Cys Arg Ser Ser Ser Gly Ala Val Thr Thr Asn Asn 
            20                  25                  30          
Tyr Ala Asn Trp Val Gln Glu Lys Pro Asp His Leu Phe Thr Gly Leu 
        35                  40                  45              
Ile Gly Gly Thr Asn Asn Arg Ala Pro Gly Val Pro Ala Arg Phe Ser 
    50                  55                  60                  
Gly Ser Leu Ile Gly Asp Lys Ala Ala Leu Thr Ile Thr Gly Ala Gln 
65                  70                  75                  80  
Thr Glu Asp Glu Ala Ile Tyr Phe Cys Ala Leu Trp Phe Ser Asp His 
                85                  90                  95      
Trp Val Phe Gly Gly Gly Thr Glu Leu Thr Val Leu Gly Arg Thr Val 
            100                 105                 110         
Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys 
        115                 120                 125             
Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg 
    130                 135                 140                 
Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn 
145                 150                 155                 160 
Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser 
                165                 170                 175     
Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys 
            180                 185                 190         
Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr 
        195                 200                 205             
Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215     

<210> 25
<211> 355
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-CD47 VHH (anti-DOTA anti-CD47 bispecific)

<400> 25
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Glu Pro Gly Gly 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ile Ile Phe Lys Ile Asn 
            20                  25                  30          
Asp Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Arg Arg Glu Trp Val 
        35                  40                  45              
Ala Ala Ser Thr Gly Gly Asp Glu Ala Ile Tyr Arg Asp Ser Val Lys 
    50                  55                  60                  
Asp Arg Phe Thr Ile Ser Arg Asp Ala Lys Asn Ser Val Phe Leu Gln 
65                  70                  75                  80  
Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Thr Ala 
                85                  90                  95      
Val Ile Ser Thr Asp Arg Asp Gly Thr Glu Trp Arg Arg Tyr Trp Gly 
            100                 105                 110         
Gln Gly Thr Gln Val Thr Val Ser Ser Gly Gly Gly Gly Gly Gly Ser 
        115                 120                 125             
Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
    130                 135                 140                 
Gly Pro Asp Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
145                 150                 155                 160 
Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
                165                 170                 175     
Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
            180                 185                 190         
His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
        195                 200                 205             
Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
    210                 215                 220                 
Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Glu 
225                 230                 235                 240 
Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
                245                 250                 255     
Cys Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 
            260                 265                 270         
Leu Ser Cys Ala Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
        275                 280                 285             
Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
    290                 295                 300                 
Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Val Ser Lys Leu Thr Val 
305                 310                 315                 320 
Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Leu 
                325                 330                 335     
His Glu Ala Leu His Ser Arg Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
            340                 345                 350         
Pro Gly Lys 
        355 

<210> 26
<211> 449
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-OAcGD2 HC (anti-OAcGD2 anti-DOTA bispecific)

<400> 26
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Ala Thr Ser Glu Phe Thr Phe Ser Asp Tyr 
            20                  25                  30          
Tyr Met Thr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              
Gly Phe Ile Arg Asn Lys Ala Asn Ser Tyr Thr Thr Glu Tyr Asn Pro 
    50                  55                  60                  
Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Ser 
65                  70                  75                  80  
Leu Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Val Tyr 
                85                  90                  95      
Tyr Cys Ala Arg Val Ser Asn Trp Ala Phe Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         
Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe 
        115                 120                 125             
Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu 
    130                 135                 140                 
Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp 
145                 150                 155                 160 
Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu 
                165                 170                 175     
Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser 
            180                 185                 190         
Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro 
        195                 200                 205             
Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys 
    210                 215                 220                 
Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro 
225                 230                 235                 240 
Asp Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser 
                245                 250                 255     
Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp 
            260                 265                 270         
Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn 
        275                 280                 285             
Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val 
    290                 295                 300                 
Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu 
305                 310                 315                 320 
Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Glu Glu Lys 
                325                 330                 335     
Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 
            340                 345                 350         
Leu Pro Pro Cys Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Trp 
        355                 360                 365             
Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
    370                 375                 380                 
Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
385                 390                 395                 400 
Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys 
                405                 410                 415     
Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Leu His Glu 
            420                 425                 430         
Ala Leu His Ser His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
        435                 440                 445             
Lys 
    

<210> 27
<211> 219
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-OAcGD2 LC (anti-OAcGD2 anti-DOTA bispecific)

<400> 27
Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Leu Gly 
1               5                   10                  15      
Gln Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Leu Lys Asn 
            20                  25                  30          
Asn Gly Asn Thr Phe Leu His Trp Tyr Gln Gln Arg Pro Gly Gln Ser 
        35                  40                  45              
Pro Arg Leu Leu Ile Tyr Lys Val Ser Asn Arg Leu Ser Gly Val Pro 
    50                  55                  60                  
Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  
Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Phe Cys Ser Gln Ser 
                85                  90                  95      
Thr His Ile Pro Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110         
Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
        115                 120                 125             
Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
    130                 135                 140                 
Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
145                 150                 155                 160 
Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
                165                 170                 175     
Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
            180                 185                 190         
Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
        195                 200                 205             
Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215                 

<210> 28
<211> 453
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-DOTA HC (anti-OAcGD2 anti-DOTA bispecific)

<400> 28
Glu Val Met Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly 
1               5                   10                  15      
Ser Leu Thr Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Thr Ala His 
            20                  25                  30          
Ala Met Ser Trp Val Arg Gln Thr Pro Glu Lys Arg Leu Glu Trp Val 
        35                  40                  45              
Ala Thr Ile Ser Gly Gly Gly Thr Tyr Thr Tyr Phe Pro Asp Ser Phe 
    50                  55                  60                  
Gln Gly Arg Phe Thr Ile Ser Ser Asp Asn Ala Lys Asn Thr Leu Tyr 
65                  70                  75                  80  
Leu Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ser Met Tyr Phe Cys 
                85                  90                  95      
Thr Arg His Gly Asp Tyr Arg Tyr Ala Phe Gly Tyr Trp Gly Gln Gly 
            100                 105                 110         
Thr Leu Val Thr Val Ser Ala Arg Thr Val Ala Ala Pro Ser Val Phe 
        115                 120                 125             
Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val 
    130                 135                 140                 
Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp 
145                 150                 155                 160 
Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr 
                165                 170                 175     
Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr 
            180                 185                 190         
Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val 
        195                 200                 205             
Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly 
    210                 215                 220                 
Glu Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu 
225                 230                 235                 240 
Leu Gly Gly Pro Asp Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
                245                 250                 255     
Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
            260                 265                 270         
Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val 
        275                 280                 285             
Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 
    290                 295                 300                 
Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
305                 310                 315                 320 
Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala 
                325                 330                 335     
Pro Glu Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
            340                 345                 350         
Gln Val Cys Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln 
        355                 360                 365             
Val Ser Leu Ser Cys Ala Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
    370                 375                 380                 
Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
385                 390                 395                 400 
Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Val Ser Lys Leu 
                405                 410                 415     
Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser 
            420                 425                 430         
Val Leu His Glu Ala Leu His Ser Arg Tyr Thr Gln Lys Ser Leu Ser 
        435                 440                 445             
Leu Ser Pro Gly Lys 
    450             

<210> 29
<211> 212
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-DOTA LC (anti-OAcGD2 anti-DOTA bispecific)

<400> 29
Gln Ala Val Thr Gln Glu Ser Ala Leu Thr Thr Ser Pro Gly Glu Thr 
1               5                   10                  15      
Val Thr Leu Thr Cys Arg Ser Ser Ser Gly Ala Val Thr Thr Asn Asn 
            20                  25                  30          
Tyr Ala Asn Trp Val Gln Glu Lys Pro Asp His Leu Phe Thr Gly Leu 
        35                  40                  45              
Ile Gly Gly Thr Asn Asn Arg Ala Pro Gly Val Pro Ala Arg Phe Ser 
    50                  55                  60                  
Gly Ser Leu Ile Gly Asp Lys Ala Ala Leu Thr Ile Thr Gly Ala Gln 
65                  70                  75                  80  
Thr Glu Asp Glu Ala Ile Tyr Phe Cys Ala Leu Trp Phe Ser Asp His 
                85                  90                  95      
Trp Val Phe Gly Gly Gly Thr Glu Leu Thr Val Leu Gly Ala Ser Thr 
            100                 105                 110         
Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser 
        115                 120                 125             
Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu 
    130                 135                 140                 
Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His 
145                 150                 155                 160 
Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser 
                165                 170                 175     
Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys 
            180                 185                 190         
Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu 
        195                 200                 205             
Pro Lys Ser Cys 
    210         

<210> 30
<211> 449
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-OAcGD2 HC (monospecific)

<400> 30
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Ala Thr Ser Glu Phe Thr Phe Ser Asp Tyr 
            20                  25                  30          
Tyr Met Thr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              
Gly Phe Ile Arg Asn Lys Ala Asn Ser Tyr Thr Thr Glu Tyr Asn Pro 
    50                  55                  60                  
Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Ser 
65                  70                  75                  80  
Leu Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Val Tyr 
                85                  90                  95      
Tyr Cys Ala Arg Val Ser Asn Trp Ala Phe Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         
Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe 
        115                 120                 125             
Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu 
    130                 135                 140                 
Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp 
145                 150                 155                 160 
Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu 
                165                 170                 175     
Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser 
            180                 185                 190         
Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro 
        195                 200                 205             
Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys 
    210                 215                 220                 
Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro 
225                 230                 235                 240 
Asp Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser 
                245                 250                 255     
Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp 
            260                 265                 270         
Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn 
        275                 280                 285             
Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val 
    290                 295                 300                 
Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu 
305                 310                 315                 320 
Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Glu Glu Lys 
                325                 330                 335     
Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 
            340                 345                 350         
Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr 
        355                 360                 365             
Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
    370                 375                 380                 
Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
385                 390                 395                 400 
Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys 
                405                 410                 415     
Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Leu His Glu 
            420                 425                 430         
Ala Leu His Ser His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
        435                 440                 445             
Lys 
    

<210> 31
<211> 219
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-OAcGD2 LC (monospecific)

<400> 31
Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Leu Gly 
1               5                   10                  15      
Gln Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Leu Lys Asn 
            20                  25                  30          
Asn Gly Asn Thr Phe Leu His Trp Tyr Gln Gln Arg Pro Gly Gln Ser 
        35                  40                  45              
Pro Arg Leu Leu Ile Tyr Lys Val Ser Asn Arg Leu Ser Gly Val Pro 
    50                  55                  60                  
Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  
Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Phe Cys Ser Gln Ser 
                85                  90                  95      
Thr His Ile Pro Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110         
Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
        115                 120                 125             
Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
    130                 135                 140                 
Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
145                 150                 155                 160 
Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
                165                 170                 175     
Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
            180                 185                 190         
Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
        195                 200                 205             
Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215                 

<210> 32
<211> 449
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-DOTA HC (monospecific)

<400> 32
Glu Val Met Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly 
1               5                   10                  15      
Ser Leu Thr Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Thr Ala His 
            20                  25                  30          
Ala Met Ser Trp Val Arg Gln Thr Pro Glu Lys Arg Leu Glu Trp Val 
        35                  40                  45              
Ala Thr Ile Ser Gly Gly Gly Thr Tyr Thr Tyr Phe Pro Asp Ser Phe 
    50                  55                  60                  
Gln Gly Arg Phe Thr Ile Ser Ser Asp Asn Ala Lys Asn Thr Leu Tyr 
65                  70                  75                  80  
Leu Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ser Met Tyr Phe Cys 
                85                  90                  95      
Thr Arg His Gly Asp Tyr Arg Tyr Ala Phe Gly Tyr Trp Gly Gln Gly 
            100                 105                 110         
Thr Leu Val Thr Val Ser Ala Ala Ser Thr Lys Gly Pro Ser Val Phe 
        115                 120                 125             
Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu 
    130                 135                 140                 
Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp 
145                 150                 155                 160 
Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu 
                165                 170                 175     
Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser 
            180                 185                 190         
Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro 
        195                 200                 205             
Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys 
    210                 215                 220                 
Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro 
225                 230                 235                 240 
Asp Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser 
                245                 250                 255     
Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp 
            260                 265                 270         
Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn 
        275                 280                 285             
Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val 
    290                 295                 300                 
Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu 
305                 310                 315                 320 
Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Glu Glu Lys 
                325                 330                 335     
Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 
            340                 345                 350         
Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr 
        355                 360                 365             
Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
    370                 375                 380                 
Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
385                 390                 395                 400 
Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys 
                405                 410                 415     
Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Leu His Glu 
            420                 425                 430         
Ala Leu His Ser His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
        435                 440                 445             
Lys 
    

<210> 33
<211> 216
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-DOTA LC (monospecific)

<400> 33
Gln Ala Val Thr Gln Glu Ser Ala Leu Thr Thr Ser Pro Gly Glu Thr 
1               5                   10                  15      
Val Thr Leu Thr Cys Arg Ser Ser Ser Gly Ala Val Thr Thr Asn Asn 
            20                  25                  30          
Tyr Ala Asn Trp Val Gln Glu Lys Pro Asp His Leu Phe Thr Gly Leu 
        35                  40                  45              
Ile Gly Gly Thr Asn Asn Arg Ala Pro Gly Val Pro Ala Arg Phe Ser 
    50                  55                  60                  
Gly Ser Leu Ile Gly Asp Lys Ala Ala Leu Thr Ile Thr Gly Ala Gln 
65                  70                  75                  80  
Thr Glu Asp Glu Ala Ile Tyr Phe Cys Ala Leu Trp Phe Ser Asp His 
                85                  90                  95      
Trp Val Phe Gly Gly Gly Thr Glu Leu Thr Val Leu Gly Arg Thr Val 
            100                 105                 110         
Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys 
        115                 120                 125             
Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg 
    130                 135                 140                 
Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn 
145                 150                 155                 160 
Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser 
                165                 170                 175     
Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys 
            180                 185                 190         
Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr 
        195                 200                 205             
Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215     

<210> 34
<211> 355
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-CD47 HC (monospecific)

<400> 34
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Glu Pro Gly Gly 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ile Ile Phe Lys Ile Asn 
            20                  25                  30          
Asp Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Arg Arg Glu Trp Val 
        35                  40                  45              
Ala Ala Ser Thr Gly Gly Asp Glu Ala Ile Tyr Arg Asp Ser Val Lys 
    50                  55                  60                  
Asp Arg Phe Thr Ile Ser Arg Asp Ala Lys Asn Ser Val Phe Leu Gln 
65                  70                  75                  80  
Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Thr Ala 
                85                  90                  95      
Val Ile Ser Thr Asp Arg Asp Gly Thr Glu Trp Arg Arg Tyr Trp Gly 
            100                 105                 110         
Gln Gly Thr Gln Val Thr Val Ser Ser Gly Gly Gly Gly Gly Gly Ser 
        115                 120                 125             
Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
    130                 135                 140                 
Gly Pro Asp Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
145                 150                 155                 160 
Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
                165                 170                 175     
Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
            180                 185                 190         
His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
        195                 200                 205             
Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
    210                 215                 220                 
Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Glu 
225                 230                 235                 240 
Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
                245                 250                 255     
Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 
            260                 265                 270         
Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
        275                 280                 285             
Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
    290                 295                 300                 
Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
305                 310                 315                 320 
Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Leu 
                325                 330                 335     
His Glu Ala Leu His Ser His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
            340                 345                 350         
Pro Gly Lys 
        355 

<210> 35
<211> 8
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-CD47 VHH-CDR1

<400> 35
Gly Ile Ile Phe Lys Ile Asn Asp 
1               5               

<210> 36
<211> 8
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-CD47 VHH-CDR2

<400> 36
Ala Ser Thr Gly Gly Asp Glu Ala 
1               5               

<210> 37
<211> 15
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-CD47 VHH-CDR3

<400> 37
Thr Ala Val Ile Ser Thr Asp Arg Asp Gly Thr Glu Trp Arg Arg 
1               5                   10                  15  

<210> 38
<211> 121
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-CD47 VHH

<400> 38
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Glu Pro Gly Gly 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ile Ile Phe Lys Ile Asn 
            20                  25                  30          
Asp Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Arg Arg Glu Trp Val 
        35                  40                  45              
Ala Ala Ser Thr Gly Gly Asp Glu Ala Ile Tyr Arg Asp Ser Val Lys 
    50                  55                  60                  
Asp Arg Phe Thr Ile Ser Arg Asp Ala Lys Asn Ser Val Phe Leu Gln 
65                  70                  75                  80  
Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Thr Ala 
                85                  90                  95      
Val Ile Ser Thr Asp Arg Asp Gly Thr Glu Trp Arg Arg Tyr Trp Gly 
            100                 105                 110         
Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120     

