                         SEQUENCE LISTING

<110>  Nanjing GenScript Biotech Co., Ltd.
 
<120>  PLASMID SYSTEM WITHOUT SELECTABLE MARKERS AND PRODUCTION METHOD THEREOF

<130>  RS-C21276WO

<150>  CN202110413747.X
<151>  2021-04-16

<160>  50    

<170>  PatentIn version 3.5

<210>  1
<211>  1050
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cre gene

<400>  1
caccatcacc atcaccatat gtccaattta ctgaccgtac accaaaattt gcctgcatta       60

ccggtcgatg caacgagtga tgaggttcgc aagaacctga tggacatgtt cagggatcgc      120

caggcgtttt ctgagcatac ctggaaaatg cttctgtccg tttgccggtc gtgggcggca      180

tggtgcaagt tgaataaccg gaaatggttt cccgcagaac ctgaagatgt tcgcgattat      240

cttctatatc ttcaggcgcg cggtctggca gtaaaaacta tccagcaaca tttgggccag      300

ctaaacatgc ttcatcgtcg gtccgggctg ccacgaccaa gtgacagcaa tgctgtttca      360

ctggttatgc ggcggatccg aaaagaaaac gttgatgccg gtgaacgtgc aaaacaggct      420

ctagcgttcg aacgcactga tttcgaccag gttcgttcac tcatggaaaa tagcgatcgc      480

tgccaggata tacgtaatct ggcatttctg gggattgctt ataacaccct gttacgtata      540

gccgaaattg ccaggatcag ggttaaagat atctcacgta ctgacggtgg gagaatgtta      600

atccatattg gcagaacgaa aacgctggtt agcaccgcag gtgtagagaa ggcacttagc      660

ctgggggtaa ctaaactggt cgagcgatgg atttccgtct ctggtgtagc tgatgatccg      720

aataactacc tgttttgccg ggtcagaaaa aatggtgttg ccgcgccatc tgccaccagc      780

cagctatcaa ctcgcgccct ggaagggatt tttgaagcaa ctcatcgatt gatttacggc      840

gctaaggatg actctggtca gagatacctg gcctggtctg gacacagtgc ccgtgtcgga      900

gccgcgcgag atatggcccg cgctggagtt tcaataccgg agatcatgca agctggtggc      960

tggaccaatg taaatattgt catgaactat atccgtaacc tggatagtga aacaggggca     1020

atggtgcgcc tgctggaaga tggcgattaa                                      1050


<210>  2
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification primer of cre gene cre-F

<400>  2
ctgtttctcc atacccgttt ttttgggatg caccatcacc atcaccatat gtccaattta       60

ctga                                                                    64


<210>  3
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification primer of cre gene cre-R

<400>  3
ggggattcag taacattcac gccggaagtg gaattcttaa tcgccatctt ccagca           56


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequencing primer of pSC101-araBAD-cre(Amp) plasmid cre-seq1

<400>  4
attatttgca cggcgtcaca                                                   20


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequencing primer of pSC101-araBAD-cre(Amp) plasmid cre-seq2

<400>  5
aacgggcatt tcagttcaag                                                   20


<210>  6
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification primer of pSC101 ori (ts) fragment pSC101(ts)-F

<400>  6
gccaatacca gtagaaacag acgaagaatc ggtatggaca gttttccctt                  50


<210>  7
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification primer of pSC101 ori (ts) fragment pSC101(ts)-R

<400>  7
aaaggaatat tcagcgattt gcccgattgc gcaaccgagc ttgcgagggt                  50


<210>  8
<211>  3191
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMF9-loxp precursor empty vector plasmid

<400>  8
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa       60

ttgctaacgc agtcaggcac cgtgtatgga agccggcggc acctcgctaa cggattcacc      120

actccaagaa ttggagccaa tcaattcttg cggagaactg tgaatgcgca aaccaaccct      180

tggcagaaca tatccatcgc gtccgccatc tccagcagcc gcacgcggcg catctcgggc      240

agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta      300

ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga gcgaacgtga      360

agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt cttcggtttc      420

cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct gcaccattat gttccggatc      480

tgcatcgcag gatgctgctg gctaccctgt ggaacaccta catctgtatt aacgaagcgc      540

tggcattgac cctgagtgat ttttctctgg tcccgccgca tccataccgc cagttgttta      600

ccctcacaac gttccagtaa ccgggcatgt tcatcatcag taacccgtat cgtgagcatc      660

ctctctcgtt tcatcggtat cattaccccc atgaacagaa atccccctta cacggaggca      720

tcagtgacca aacaggaaaa aaccgccctt aacatggccc gctttatcag aagccagaca      780

ttaacgcttc tggagaaact caacgagctg gacgcggatg aacaggcaga catctgtgaa      840

tcgcttcacg accacgctga tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac      900

ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat      960

gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca     1020

gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag     1080

agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga     1140

gaaaataccg catcaggata acttcgtata atgtatgcta tacgaacggt acgctcttcc     1200

gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct     1260

cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg     1320

tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc     1380

cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga     1440

aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct     1500

cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg     1560

gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag     1620

ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat     1680

cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac     1740

aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac     1800

tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc     1860

ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt     1920

tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tccttgtaaa     1980

acgacggcca gtgaattcga gctcggtacc cggggatcct ctagagtcga cctgcaggca     2040

tgcaagcttg gcgtaatcat ggtcatagct gtttcctgta ccgttcgtat aatgtatgct     2100

atacgaagtt attgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt     2160

aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa     2220

aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttagaaaa     2280

actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt     2340

tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg     2400

caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt     2460

tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg     2520

gtgagaatgg caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac     2580

gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag     2640

cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc     2700

ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta     2760

atacctggaa tgctgttttc ccggggatcg cagtggtgag taaccatgca tcatcaggag     2820

tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga     2880

ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg     2940

gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc     3000

gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctagagc     3060

aagacgtttc ccgttgaata tggctcatca catttccccg aaaagtgcca cctgacgtct     3120

aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc     3180

gtcttcaaga a                                                          3191


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNAII

<400>  9
gatgaacgtg ccggaaagcg                                                   20


<210>  10
<211>  499
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  JM108-araBAD-cre(Amp) L-arm

<400>  10
gggggttgtt cttcgaacgc ctgaatgtat cggtacagaa actggaaaac gccatcgagt       60

tttatggcgc gatttcgcat aacaaactgc acggcctgtc agtgcaggtt gaaaccaatc      120

acgtcaaatt accggcggtg gtggacggct ttgccagcga agggatcatc cagttctttt      180

tcgaagaaac gcaagacgag aatggcttta atatctacat tctcgacgaa agcaaccggg      240

ttgaggtata tcaccactgc gaaggcagca aagaggagct ggtacgtgac gtcagtcgct      300

tctactcgtc atcgcatgac cgttttacct acggctcaag cttcatcaac ttcaacctgc      360

cgcagttcta tcagattgtg aaggttgatg gtcgtgaaca ggtgattccg ttccgcacaa      420

aatctatcgg taacatgccg cctgccaatc aggatcacga tacgccgcta ttacagcaat      480

atttttcgtg atgaacgtg                                                   499


<210>  11
<211>  496
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  JM108-araBAD-cre(Amp)R-arm

<400>  11
atgcaatctt agcggaaact gactgtttca cccgcctgct gcgtcgccgc ctgttccagc       60

aaatcccaga aggtttcgcc gctgcgatca caaatccact catcgccttt caggtcaaaa      120

tggtagccgc cctgtttggt tgccagccat acctggtgca gcggctcctg gcggttgata      180

atgattttgc tgccattctc aaaggtaatg gtcagtacgc cgccgttgat ttcgcagtcg      240

atatcgctgt cgccatccca gtcgtccagg cgttcttcaa tggtcagcca gagttgatca      300

gccaggcgat gaaattcact gtcgttcatt gttgtatcct gtttttaagt gatggcggca      360

gtatagcggc atggggtcag ggcttcaaag tttgcacctc tgcggctgcg ttccggcacg      420

attcatccgt caccggaata atgatgtctc tgtgtagcga aagatttgtc tcttcattag      480

ggcgcagtta caccac                                                      496


<210>  12
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification primer of 5.5 kb fragment 5.5kb-F

<400>  12
aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atccttgtaa aacgacggcc       60

agtgaattcg gacattgatt attgactagt                                        90


<210>  13
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification primer of 5.5 kb fragment 5.5kb-R

<400>  13
acgaacggta caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc       60

gactctagag ccatagagcc caccgcatcc                                        90


<210>  14
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Bacteria detection primer of strain JM108 araBAD-cre after gene 
       editing C4818FK060-6JJF1

<400>  14
agcgtggaag tcttctgtta tagcca                                            26


<210>  15
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Bacteria detection primer of strain JM108 araBAD-cre after gene 
       editing C4818FK060-6JJR1

<400>  15
acctcgcgcc aggatggctt tatgcac                                           27


<210>  16
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification primer of flp gene flp-F

<400>  16
tttttttggg atgcaccatc accatcacca tatgccacaa tttgatatat                  50


<210>  17
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification primer of flp gene flp-R

<400>  17
ttcagtaaca ttcacgccgg aagtggaatt ttatatgcgt ctatttatgt                  50


<210>  18
<211>  93
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification primer of FRT-RFP fragment FRT-F1

<400>  18
cgtaaggaga aaataccgca tcagggaagt tcctatactt tctagagaat aggaacttcg       60

gaataggaac ttccgctctt ccgcttcctc gct                                    93


<210>  19
<211>  93
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification primer of FRT-RFP fragment FRT-R1

<400>  19
gcgtcagacc ccgtagaaaa gatcagaagt acctattccg aagttcctat tctctagaaa       60

gtataggaac ttccaggaaa cagctatgac cat                                    93


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification primer of FRT vector FRT-F2

<400>  20
tgatcttttc tacggggtct                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification primer of FRT vector FRT-R2

<400>  21
cctgatgcgg tattttctcc                                                   20


<210>  22
<211>  2258
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cre specific recombinase araBAD-cre inducible expression system

<400>  22
ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct       60

cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc      120

aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg      180

gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag      240

atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt      300

gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg      360

tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt      420

tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa      480

caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa      540

tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg      600

gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa      660

cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg      720

accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa      780

atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga      840

gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt      900

cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg      960

gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg     1020

gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca     1080

cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt     1140

agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt     1200

ttgggatgca ccatcaccat caccatatgt ccaatttact gaccgtacac caaaatttgc     1260

ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg gacatgttca     1320

gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt tgccggtcgt     1380

gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct gaagatgttc     1440

gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaactatc cagcaacatt     1500

tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt gacagcaatg     1560

ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt gaacgtgcaa     1620

aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc atggaaaata     1680

gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat aacaccctgt     1740

tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact gacggtggga     1800

gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt gtagagaagg     1860

cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct ggtgtagctg     1920

atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc gcgccatctg     1980

ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact catcgattga     2040

tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga cacagtgccc     2100

gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag atcatgcaag     2160

ctggtggctg gaccaatgta aatattgtca tgaactatat ccgtaacctg gatagtgaaa     2220

caggggcaat ggtgcgcctg ctggaagatg gcgattaa                             2258


<210>  23
<211>  1434
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pSC101 ori(ts) fragment

<400>  23
tatggacagt tttccctttg atatctaacg gtgaacagtt gttctacttt tgtttgttag       60

tcttgatgct tcactgatag atacaagagc cataagaacc tcagatcctt ccgtatttag      120

ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag ccatgagaac gaaccattga      180

gatcatgctt actttgcatg tcactcaaaa attttgcctc aaaactggtg agctgaattt      240

ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt acgtaggtag gaatctgatg      300

taatggttgt tggtattttg tcaccattca tttttatctg gttgttctca agttcggtta      360

cgagatccat ttgtctatct agttcaactt ggaaaatcaa cgtatcagtc gggcggcctc      420

gcttatcaac caccaatttc atattgctgt aagtgtttaa atctttactt attggtttca      480

aaacccattg gttaagcctt ttaaactcat ggtagttatt ttcaagcatt aacatgaact      540

taaattcatc aaggctaatc tctatatttg ccttgtgagt tttcttttgt gttagttctt      600

ttaataacca ctcataaatc ctcatagagt atttgttttc aaaagactta acatgttcca      660

gattatattt tatgaatttt tttaactgga aaagataagg caatatctct tcactaaaaa      720

ctaattctaa tttttcgctt gagaacttgg catagtttgt ccactggaaa atctcaaagc      780

ctttaaccaa aggattcctg atttccacag ttctcgtcat cagctctctg gttgctttag      840

ctaatacacc ataagcattt tccctactga tgttcatcat ctgaacgtat tggttataag      900

tgaacgatac cgtccgttct ttccttgtag ggttttcaat cgtggggttg agtagtgcca      960

cacagcataa aattagcttg gtttcatgct ccgttaagtc atagcgacta atcgctagtt     1020

catttgcttt gaaaacaact aattcagaca tacatctcaa ttggtctagg tgattttaat     1080

cactatacca attgagatgg gctagtcaat gataattact agtccttttc ctttgagttg     1140

tgggtatctg taaattctgc tagacctttg ctggaaaact tgtaaattct gctagaccct     1200

ctgtaaattc cgctagacct ttgtgtgttt tttttgttta tattcaagtg gttataattt     1260

atagaataaa gaaagaataa aaaaagataa aaagaataga tcccagccct gtgtataact     1320

cactacttta gtcagttccg cagtattaca aaaggatgtc gcaaacgctg tttgctcctc     1380

tacaaaacag accttaaaac cctaaaggct taagtagcac cctcgcaagc tcgg           1434


<210>  24
<211>  1272
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Flp gene

<400>  24
ttatatgcgt ctatttatgt aggatgaaag gtagtctagt acctcctgtg atattatccc       60

attccatgcg gggtatcgta tgcttccttc agcactaccc tttagctgtt ctatatgctg      120

ccactcctca attggattag tctcatcctt caatgctatc atttcctttg atattggatc      180

atatgcatag taccgagaaa ctagtgcgaa gtagtgatca ggtattgctg ttatctgatg      240

agtatacgtt gtcctggcca cggcagaagc acgcttatcg ctccaatttc ccacaacatt      300

agtcaactcc gttaggccct tcattgaaag aaatgaggtc atcaaatgtc ttccaatgtg      360

agattttggg ccatttttta tagcaaagat tgaataaggc gcatttttct tcaaagcttt      420

attgtacgat ctgactaagt tatcttttaa taattggtat tcctgtttat tgcttgaaga      480

attgccggtc ctatttactc gttttaggac tggttcagaa ttcctcaaaa attcatccaa      540

atatacaagt ggatcgatcc taccccttgc gctaaagaag tatatgtgcc tactaacgct      600

tgtctttgtc tctgtcacta aacactggat tattactccc agatacttat tttggactaa      660

tttaaatgat ttcggatcaa cgttcttaat atcgctgaat cttccacaat tgatgaaagt      720

agctaggaag aggaattggt ataaagtttt tgtttttgta aatctcgaag tatactcaaa      780

cgaatttagt attttctcag tgatctccca gatgctttca ccctcactta gaagtgcttt      840

aagcattttt ttactgtggc tatttccctt atctgcttct tccgatgatt cgaactgtaa      900

ttgcaaacta cttacaatat cagtgatatc agattgatgt ttttgtccat agtaaggaat      960

aattgtaaat tcccaagcag gaatcaattt ctttaatgag gcttccagaa ttgttgcttt     1020

ttgcgtcttg tatttaaact ggagtgattt attgacaata tcgaaactca gcgaattgct     1080

tatgatagta ttatagctca tgaatgtggc tctcttgatt gctgttccgt tatgtgtaat     1140

catccaacat aaataggtta gttcagcagc acataatgct attttctcac ctgaaggtct     1200

ttcaaacctt tccacaaact gacgaacaag caccttaggt ggtgttttac ataatatacc     1260

aaattgtggc at                                                         1272


<210>  25
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lox71

<400>  25
taccgttcgt ataatgtatg ctatacgaag ttat                                   34


<210>  26
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lox66

<400>  26
ataacttcgt ataatgtatg ctatacgaac ggta                                   34


<210>  27
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FRT

<400>  27
gaagttccta tactttctag agaataggaa cttcggaata ggaacttc                    48


<210>  28
<211>  1090
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rfp gene

<400>  28
gtacccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca       60

cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct      120

cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat      180

tgtgagcgga taacaatttc acacatacta gagaaagagg agaaatacta gatggcttcc      240

tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac      300

ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc      360

gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg      420

cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg      480

aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt      540

gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa      600

ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg      660

gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg      720

cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct      780

aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc      840

cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc      900

ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg catcaaataa      960

aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg     1020

ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatagtcgac     1080

tcgagcggcc                                                            1090


<210>  29
<211>  2579
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pUC57(KanR)

<400>  29
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagagaatt cgagctcggt acctcgcgaa      420

tacatctaga tatcggatcc cgggcccgtc gactgcagag gcctgcatgc aagcttggtg      480

taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac      540

atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca      600

ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat      660

taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc      720

tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca      780

aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca      840

aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg      900

ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg      960

acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt     1020

ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt     1080

tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc     1140

tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt     1200

gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt     1260

agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc     1320

tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa     1380

agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt     1440

tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct     1500

acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta     1560

tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaagccca     1620

atctgaataa tgttacaacc aattaaccaa ttctgattag aaaaactcat cgagcatcaa     1680

atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt     1740

ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg     1800

gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat     1860

aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag     1920

tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc     1980

actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg     2040

atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc     2100

cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt     2160

ttttccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt     2220

gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac     2280

atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc     2340

atacaagcga tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc     2400

atataaatca gcatccatgt tggaatttaa tcgcggcctc gacgtttccc gttgaatatg     2460

gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga     2520

tatattttta tcttgtgcaa tgtaacatca gagattttga gacacgggcc agagctgca      2579


<210>  30
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMB1 amplification primer pUC57-short-1-1

<400>  30
ataacttcgt atagcataca ttatacgaac ggtattgaga tccttttttt ctgcgcgtaa       60

tctg                                                                    64


<210>  31
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMB1 amplification primer pUC57-short-1-2

<400>  31
gctatacgaa cggtagaatt cggatcccgg gcccgtcgac tgcagtttcc ataggctccg       60

ccccc                                                                   65


<210>  32
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification primer of pUC57(KanR) fragment pUC57-short-2-1

<400>  32
ccgggatccg aattctaccg ttcgtatagc atacattata cgaagttatt ctggccgtcg       60

ttttacaacg                                                              70


<210>  33
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification primer of pUC57(KanR) fragment pUC57-short-2-2

<400>  33
gttcgtataa tgtatgctat acgaagttat gaagatcctt tgatcttttc                  50


<210>  34
<211>  2182
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMF65-loxp precursor empty vector plasmid

<400>  34
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagaataac ttcgtataat gtatgctata      420

cgaacggtag aattcggatc ccgggcccgt cgactgcagt ttccataggc tccgcccccc      480

tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata      540

aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc      600

gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc      660

acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga      720

accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc      780

ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag      840

gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag      900

aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag      960

ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca     1020

gattacgcgc agaaaaaaag gatctcaata ccgttcgtat aatgtatgct atacgaagtt     1080

atgaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt     1140

aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa     1200

aatgaagttt taaatcaagc ccaatctgaa taatgttaca accaattaac caattctgat     1260

tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata     1320

ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat     1380

aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct     1440

attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact     1500

gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag     1560

ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc     1620

gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa     1680

tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat     1740

tcttctaata cctggaatgc tgtttttccg gggatcgcag tggtgagtaa ccatgcatca     1800

tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt     1860

agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac     1920

aactctggcg catcgggctt cccatacaag cgatagattg tcgcacctga ttgcccgaca     1980

ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc     2040

ctcgacgttt cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca     2100

gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt     2160

tgagacacgg gccagagctg ca                                              2182


<210>  35
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification primer of pMF65-loxp empty vector Short-F

<400>  35
gggatccgaa ttctaccgtt cgtatagcat acattatacg aagttattct                  50


<210>  36
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification primer of pMF65-loxp empty vector Short-R

<400>  36
gggcccgtcg actgcagttt ccataggctc cgcccccctg acgagcatca caaa             54


<210>  37
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification primer of RFP fragment RFP-F

<400>  37
atgctatacg aacggtagaa ttcggatccc gtacccaata cgcaaaccgc                  50


<210>  38
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification primer of RFP fragment RFP-R

<400>  38
gagcctatgg aaactgcagt cgacgggccc ggccgctcga gtcgactata                  50


<210>  39
<211>  3879
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMF7-loxp-RFP precursor plasmid

<400>  39
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagagggtg taatcatggt catagctgtt      420

tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa      480

gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact      540

gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc      600

ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg      660

ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc      720

cacagaatca ggggataacg caggaaagaa cataacttcg tataatgtat gctatacgaa      780

cggtagaatt cggatcccgt acccaatacg caaaccgcct ctccccgcgc gttggccgat      840

tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc      900

aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc      960

tcgtatgttg tgtggaattg tgagcggata acaatttcac acatactaga gaaagaggag     1020

aaatactaga tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt     1080

atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg     1140

tacgaaggta cccagaccgc taaactgaaa gttaccaaag gtggtccgct gccgttcgct     1200

tgggacatcc tgtccccgca gttccagtac ggttccaaag cttacgttaa acacccggct     1260

gacatcccgg actacctgaa actgtccttc ccggaaggtt tcaaatggga acgtgttatg     1320

aacttcgaag acggtggtgt tgttaccgtt acccaggact cctccctgca agacggtgag     1380

ttcatctaca aagttaaact gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag     1440

aaaaaaacca tgggttggga agcttccacc gaacgtatgt acccggaaga cggtgctctg     1500

aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg gtcactacga cgctgaagtt     1560

aaaaccacct acatggctaa aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc     1620

aaactggaca tcacctccca caacgaagac tacaccatcg ttgaacagta cgaacgtgct     1680

gaaggtcgtc actccaccgg tgcttaataa cgctgatagt gctagtgtag atcgctacta     1740

gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct     1800

gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt     1860

tctgcgttta tagtcgactc gagcggccgg gcccgtcgac tgcagatgtg agcaaaaggc     1920

cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc     1980

ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga     2040

ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc     2100

ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat     2160

agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg     2220

cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc     2280

aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga     2340

gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact     2400

agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt     2460

ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag     2520

cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggt     2580

accgttcgta taatgtatgc tatacgaagt tattcagagg ttttcaccgt catcaccgaa     2640

acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc     2700

tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct     2760

gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcacgtctg acgctcagtg     2820

gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta     2880

gatcctttta aattaaaaat gaagttttaa atcaagccca atctgaataa tgttacaacc     2940

aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca     3000

tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact     3060

caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc     3120

caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat     3180

caccatgagt gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga     3240

cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt     3300

tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat     3360

tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt     3420

cacctgaatc aggatattct tctaatacct ggaatgctgt ttttccgggg atcgcagtgg     3480

tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa     3540

attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt     3600

tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaagcga tagattgtcg     3660

cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt     3720

tggaatttaa tcgcggcctc gacgtttccc gttgaatatg gctcataaca ccccttgtat     3780

tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta tcttgtgcaa     3840

tgtaacatca gagattttga gacacgggcc agagctgca                            3879


<210>  40
<211>  3369
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMF5-loxp-RFP precursor plasmid

<400>  40
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa       60

ttgctaacgc agtcaggcac cgtgtatgga agccggcggc acctcgctaa cggattcacc      120

actccaagaa ttggagccaa tcaattcttg cggagaactg tgaatgcgca aaccaaccct      180

tggcagaaca tatccatcgc gtccgccatc tccagcagcc gcacgcggcg catctcgggc      240

agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta      300

ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga gcgaacgtga      360

agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt cttcggtttc      420

cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct gcaccattat gttccggatc      480

tgcatcgcag gatgctgctg gctaccctgt ggaacaccta catctgtatt aacgaagcgc      540

tggcattgac cctgagtgat ttttctctgg tcccgccgca tccataccgc cagttgttta      600

ccctcacaac gttccagtaa ccgggcatgt tcatcatcag taacccgtat cgtgagcatc      660

ctctctcgtt tcatcggtat cattaccccc atgaacagaa atccccctta cacggaggca      720

tcaataactt cgtataatgt atgctatacg aacggtactg cagtgtcagc cgttaagtgt      780

tcctgtgtca ctcaaaattg ctttgagagg ctctaagggc ttctcagtgc gttacatccc      840

tggcttgttg tccacaaccg ttaaacctta aaagctttaa aagccttata tattcttttt      900

tttcttataa aacttaaaac cttagaggct atttaagttg ctgatttata ttaattttat      960

tgttcaaaca tgagagctta gtacgtgaaa catgagagct tagtacgtta gccatgagag     1020

cttagtacgt tagccatgag ggtttagttc gttaaacatg agagcttagt acgttaaaca     1080

tgagagctta gtacgtgaaa catgagagct tagtacgtac tatcaacagg ttgaactgct     1140

gatcttcaga tcggtaccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt     1200

aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta     1260

atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta     1320

tgttgtgtgg aattgtgagc ggataacaat ttcacacata ctagagaaag aggagaaata     1380

ctagatggct tcctccgaag acgttatcaa agagttcatg cgtttcaaag ttcgtatgga     1440

aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga     1500

aggtacccag accgctaaac tgaaagttac caaaggtggt ccgctgccgt tcgcttggga     1560

catcctgtcc ccgcagttcc agtacggttc caaagcttac gttaaacacc cggctgacat     1620

cccggactac ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt     1680

cgaagacggt ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg gtgagttcat     1740

ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa     1800

aaccatgggt tgggaagctt ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg     1860

tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac tacgacgctg aagttaaaac     1920

cacctacatg gctaaaaaac cggttcagct gccgggtgct tacaaaaccg acatcaaact     1980

ggacatcacc tcccacaacg aagactacac catcgttgaa cagtacgaac gtgctgaagg     2040

tcgtcactcc accggtgctt aataacgctg atagtgctag tgtagatcgc tactagagcc     2100

aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt     2160

ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc     2220

gtttatagtc gactcgagcg gccagtcgac gcatgctacc gttcgtataa tgtatgctat     2280

acgaagttat tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa     2340

gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa     2400

tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttagaaaaac     2460

tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt     2520

tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca     2580

agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc     2640

ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt     2700

gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc     2760

tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg     2820

agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg     2880

cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat     2940

acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta     3000

cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc     3060

atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc     3120

gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga     3180

gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg cctagagcaa     3240

gacgtttccc gttgaatatg gctcatcaca tttccccgaa aagtgccacc tgacgtctaa     3300

gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt     3360

cttcaagaa                                                             3369


<210>  41
<211>  2789
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMF7-loxp precursor empty vector plasmid

<400>  41
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagagggtg taatcatggt catagctgtt      420

tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa      480

gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact      540

gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc      600

ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg      660

ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc      720

cacagaatca ggggataacg caggaaagaa cataacttcg tataatgtat gctatacgaa      780

cggtagaatt cggatcccgg gcccgtcgac tgcagatgtg agcaaaaggc cagcaaaagg      840

ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg      900

agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat      960

accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta     1020

ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct     1080

gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc     1140

ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa     1200

gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg     1260

taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag     1320

tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt     1380

gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta     1440

cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggt accgttcgta     1500

taatgtatgc tatacgaagt tattcagagg ttttcaccgt catcaccgaa acgcgcgagg     1560

cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca     1620

tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg     1680

gccatgttaa gggcggtttt ttcctgtttg gtcacgtctg acgctcagtg gaacgaaaac     1740

tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta     1800

aattaaaaat gaagttttaa atcaagccca atctgaataa tgttacaacc aattaaccaa     1860

ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt     1920

atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca     1980

gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat     2040

acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt     2100

gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac     2160

aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg     2220

tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg     2280

aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc     2340

aggatattct tctaatacct ggaatgctgt ttttccgggg atcgcagtgg tgagtaacca     2400

tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag     2460

ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt     2520

cagaaacaac tctggcgcat cgggcttccc atacaagcga tagattgtcg cacctgattg     2580

cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa     2640

tcgcggcctc gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat     2700

gtaagcagac agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca     2760

gagattttga gacacgggcc agagctgca                                       2789


<210>  42
<211>  2289
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMF5-loxp precursor empty vector plasmid

<400>  42
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa       60

ttgctaacgc agtcaggcac cgtgtatgga agccggcggc acctcgctaa cggattcacc      120

actccaagaa ttggagccaa tcaattcttg cggagaactg tgaatgcgca aaccaaccct      180

tggcagaaca tatccatcgc gtccgccatc tccagcagcc gcacgcggcg catctcgggc      240

agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta      300

ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga gcgaacgtga      360

agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt cttcggtttc      420

cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct gcaccattat gttccggatc      480

tgcatcgcag gatgctgctg gctaccctgt ggaacaccta catctgtatt aacgaagcgc      540

tggcattgac cctgagtgat ttttctctgg tcccgccgca tccataccgc cagttgttta      600

ccctcacaac gttccagtaa ccgggcatgt tcatcatcag taacccgtat cgtgagcatc      660

ctctctcgtt tcatcggtat cattaccccc atgaacagaa atccccctta cacggaggca      720

tcaataactt cgtataatgt atgctatacg aacggtactg cagtgtcagc cgttaagtgt      780

tcctgtgtca ctcaaaattg ctttgagagg ctctaagggc ttctcagtgc gttacatccc      840

tggcttgttg tccacaaccg ttaaacctta aaagctttaa aagccttata tattcttttt      900

tttcttataa aacttaaaac cttagaggct atttaagttg ctgatttata ttaattttat      960

tgttcaaaca tgagagctta gtacgtgaaa catgagagct tagtacgtta gccatgagag     1020

cttagtacgt tagccatgag ggtttagttc gttaaacatg agagcttagt acgttaaaca     1080

tgagagctta gtacgtgaaa catgagagct tagtacgtac tatcaacagg ttgaactgct     1140

gatcttcaga tcggatcctc tagagtcgac gcatgctacc gttcgtataa tgtatgctat     1200

acgaagttat tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa     1260

gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa     1320

tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttagaaaaac     1380

tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt     1440

tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca     1500

agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc     1560

ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt     1620

gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc     1680

tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg     1740

agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg     1800

cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat     1860

acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta     1920

cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc     1980

atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc     2040

gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga     2100

gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg cctagagcaa     2160

gacgtttccc gttgaatatg gctcatcaca tttccccgaa aagtgccacc tgacgtctaa     2220

gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt     2280

cttcaagaa                                                             2289


<210>  43
<211>  784
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMB1 ori derivative (784 bp)

<400>  43
cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg       60

gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga      120

aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg      180

gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag      240

aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc      300

gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg      360

ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt      420

cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc      480

ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc      540

actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg      600

tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca      660

gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc      720

ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat      780

cctt                                                                   784


<210>  44
<211>  589
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMB1 ori (589 bp)

<400>  44
tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg       60

gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg      120

ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag      180

cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc      240

caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa      300

ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg      360

taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc      420

taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac      480

cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg      540

tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaa                  589


<210>  45
<211>  674
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pUC ori (674 bp)

<400>  45
atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt       60

ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg      120

cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc      180

tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc      240

gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc      300

aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac      360

tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt      420

aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct      480

aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc      540

ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt      600

ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg      660

atcttttcta cggg                                                        674


<210>  46
<211>  389
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  R6K ori(389bp)

<400>  46
tgtcagccgt taagtgttcc tgtgtcactc aaaattgctt tgagaggctc taagggcttc       60

tcagtgcgtt acatccctgg cttgttgtcc acaaccgtta aaccttaaaa gctttaaaag      120

ccttatatat tctttttttt cttataaaac ttaaaacctt agaggctatt taagttgctg      180

atttatatta attttattgt tcaaacatga gagcttagta cgtgaaacat gagagcttag      240

tacgttagcc atgagagctt agtacgttag ccatgagggt ttagttcgtt aaacatgaga      300

gcttagtacg ttaaacatga gagcttagta cgtgaaacat gagagcttag tacgtactat      360

caacaggttg aactgctgat cttcagatc                                        389


<210>  47
<211>  3219
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMF9-FRT precursor empty vector plasmid

<400>  47
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa       60

ttgctaacgc agtcaggcac cgtgtatgga agccggcggc acctcgctaa cggattcacc      120

actccaagaa ttggagccaa tcaattcttg cggagaactg tgaatgcgca aaccaaccct      180

tggcagaaca tatccatcgc gtccgccatc tccagcagcc gcacgcggcg catctcgggc      240

agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta      300

ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga gcgaacgtga      360

agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt cttcggtttc      420

cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct gcaccattat gttccggatc      480

tgcatcgcag gatgctgctg gctaccctgt ggaacaccta catctgtatt aacgaagcgc      540

tggcattgac cctgagtgat ttttctctgg tcccgccgca tccataccgc cagttgttta      600

ccctcacaac gttccagtaa ccgggcatgt tcatcatcag taacccgtat cgtgagcatc      660

ctctctcgtt tcatcggtat cattaccccc atgaacagaa atccccctta cacggaggca      720

tcagtgacca aacaggaaaa aaccgccctt aacatggccc gctttatcag aagccagaca      780

ttaacgcttc tggagaaact caacgagctg gacgcggatg aacaggcaga catctgtgaa      840

tcgcttcacg accacgctga tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac      900

ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat      960

gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca     1020

gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag     1080

agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga     1140

gaaaataccg catcagggaa gttcctatac tttctagaga ataggaactt cggaatagga     1200

acttccgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc     1260

gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg     1320

caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt     1380

tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa     1440

gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct     1500

ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc     1560

cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg     1620

tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct     1680

tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag     1740

cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga     1800

agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga     1860

agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg     1920

gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag     1980

aagatccttg taaaacgacg gccagtgaat tcgagctcgg tacccgggga tcctctagag     2040

tcgacctgca ggcatgcaag cttggcgtaa tcatggtcat agctgtttcc tggaagttcc     2100

tatactttct agagaatagg aacttcggaa taggtacttc tgatcttttc tacggggtct     2160

gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg     2220

atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat     2280

gagtaaactt ggtctgacag ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta     2340

ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa     2400

aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact     2460

cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag     2520

aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc     2580

cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa     2640

ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga     2700

caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata     2760

ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc ggggatcgca     2820

gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc     2880

ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta     2940

cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt     3000

gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc     3060

atgttggaat ttaatcgcgg cctagagcaa gacgtttccc gttgaatatg gctcatcaca     3120

tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat     3180

aaaaataggc gtatcacgag gccctttcgt cttcaagaa                            3219


<210>  48
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  attB sequence

<400>  48
ggtgccaggg cgtgcccttg ggctccccgg gcgcg                                  35


<210>  49
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  attP sequence

<400>  49
cccaactggg gtaacctttg agttctctca gttgggg                                37


<210>  50
<211>  1818
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  phiC31:( protein ID: ADD10761.1)

<400>  50
gtggacacgt acgcgggtgc ttacgaccgt cagtcgcgcg agcgcgaaaa ttcgagcgca       60

gcaagcccag cgacacagcg tagcgccaac gaagacaagg cggccgacct tcagcgcgaa      120

gtcgagcgcg acgggggccg gttcaggttc gtcgggcatt tcagcgaagc gccgggcacg      180

tcggcgttcg ggacggcgga gcgcccggag ttcgaacgca tcctgaacga atgccgcgcc      240

gggcggctca acatgatcat tgtctatgac gtgtcgcgct tctcgcgcct gaaggtcatg      300

gacgcgattc cgattgtctc ggaattgctc gccctgggcg tgacgattgt ttccactcag      360

gaaggcgtct tccggcaggg aaacgtcatg gacctgattc acctgattat gcggctcgac      420

gcgtcgcaca aagaatcttc gctgaagtcg gcgaagattc tcgacacgaa gaaccttcag      480

cgcgaattgg gcgggtacgt cggcgggaag gcgccttacg gcttcgagct tgtttcggag      540

acgaaggaga tcacgcgcaa cggccgaatg gtcaatgtcg tcatcaacaa gcttgcgcac      600

tcgaccactc cccttaccgg acccttcgag ttcgagcccg acgtaatccg gtggtggtgg      660

cgtgagatca agacgcacaa acaccttccc ttcaagccgg gcagtcaagc cgccattcac      720

ccgggcagca tcacggggct ttgtaagcgc atggacgctg acgccgtgcc gacccggggc      780

gagacgattg ggaagaagac cgcttcaagc gcctgggacc cggcaaccgt tatgcgaatc      840

cttcgggacc cgcgtattgc gggcttcgcc gctgaggtga tctacaagaa gaagccggac      900

ggcacgccga ccacgaagat tgagggttac cgcattcagc gcgacccgat cacgctccgg      960

ccggtcgagc ttgattgcgg accgatcatc gagcccgctg agtggtatga gcttcaggcg     1020

tggttggacg gcagggggcg cggcaagggg ctttcccggg ggcaagccat tctgtccgcc     1080

atggacaagc tgtactgcga gtgtggcgcc gtcatgactt cgaagcgcgg ggaagaatcg     1140

atcaaggact cttaccgctg ccgtcgccgg aaggtggtcg acccgtccgc acctgggcag     1200

cacgaaggca cgtgcaacgt cagcatggcg gcactcgaca agttcgttgc ggaacgcatc     1260

ttcaacaaga tcaggcacgc cgaaggcgac gaagagacgt tggcgcttct gtgggaagcc     1320

gcccgacgct tcggcaagct cactgaggcg cctgagaaga gcggcgaacg ggcgaacctt     1380

gttgcggagc gcgccgacgc cctgaacgcc cttgaagagc tgtacgaaga ccgcgcggca     1440

ggcgcgtacg acggacccgt tggcaggaag cacttccgga agcaacaggc agcgctgacg     1500

ctccggcagc aaggggcgga agagcggctt gccgaacttg aagccgccga agccccgaag     1560

cttccccttg accaatggtt ccccgaagac gccgacgctg acccgaccgg ccctaagtcg     1620

tggtgggggc gcgcgtcagt agacgacaag cgcgtgttcg tcgggctctt cgtagacaag     1680

atcgttgtca cgaagtcgac tacgggcagg gggcagggaa cgcccatcga gaagcgcgct     1740

tcgatcacgt gggcgaagcc gccgaccgac gacgacgaag acgacgccca ggacggcacg     1800

gaagacgtag cggcgtag                                                   1818


