                         SEQUENCE LISTING

<110>  SHENZHEN UNIVERSITY
 
<120>  GLB1 RECOMBINANT REPORTER GENE, ISOLATED CONSTRUCT, AND METHOD 
       FOR PREPARING TRANSGENIC REPORTER MOUSE

<130>  PCT214993

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  1324
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Glb1-2A-mCherry, GAC

<400>  1
gatgctggac tctatggggc atctgttgcc tctctgatac tagtgttcct cagctgacaa       60

cctctctctc tctctccctc tctctctctc tcttcctctc tctccctctc tctctctctc      120

tctctctctc tctctagggt caagtatgga tcaatggctt taacctcggc cgatactggc      180

ccacaatggg cccacaaaag accttgttcg tgccaaggaa catcctgacc acttcagccc      240

caaacaacat cacagtgttg gagctagagt ttgcaccctg cagcgagggg accccagagc      300

tgtgtacagt agagtttgtt gacactccgg tcatttccgg tagcggagct actaacttca      360

gcctgctgaa gcaggctggc gacgtggagg agaaccctgg tcctatggtg agcaagggcg      420

aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct      480

ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca      540

cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc      600

tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg      660

actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg      720

acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca      780

aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca      840

tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga      900

tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct      960

acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca     1020

tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc     1080

actccaccgg cggcatggac gagctgtaca agtaactgtg agcactgaac gcctactcga     1140

gccctcagtc tcctgtcaag gacttgacca tcggtggcca ttttccaagc cagtctggtc     1200

aagactcatg gctgaacctc tgagactgag ccttggggag cacagctcta ctctggttac     1260

acggatcacc tttgttgtgc tagaatggaa gctacatttc tggaatgtgt gtaccctgct     1320

ggtg                                                                  1324


<210>  2
<211>  1944
<212>  DNA
<213>  Mus musculus

<400>  2
atgctccggg tccccctgtg tacgccgctc ccgctcctgg cactgctgca actgctgggc       60

gctgcgcacg gcatctataa tgtcacccag aggacattta agctcgacta cagccgggac      120

cgcttcctca aggatggaca gccattccga tacatctcgg gaagcattca ttacttccgg      180

ataccccgct tctactggga ggaccggctg ctgaagatga agatggctgg gctgaatgct      240

atccagatgt acgtgccctg gaacttccat gaaccccaac caggacaata tgagttttct      300

ggggaccgtg atgtggagca tttcatccag ctggctcatg agctgggact cctggtgatc      360

ctgaggcctg ggccctacat ctgtgcagag tgggacatgg ggggcttacc tgcttggcta      420

ctagagaaac aatctatcgt tctccggtct tctgacccag actaccttgt agctgtggat      480

aaatggctgg cagtccttct gcccaagatg aagcccctgc tctaccagaa cggaggaccg      540

atcataaccg tgcaggttga gaatgagtac gggtcctact ttgcctgcga ttacgactac      600

ctacgcttcc tggtgcaccg cttccgctac catctgggta atgacgtcat tctcttcacc      660

accgacggag caagtgaaaa aatgctgaag tgtgggaccc tgcaggacct gtacgccaca      720

gtggattttg gaacaggcaa caatatcaca caagctttcc tggtccagag gaagtttgaa      780

cctaaaggac ctttgatcaa ttctgagttc tatactggct ggctagacca ctggggtaaa      840

ccccattcca cggtgaaaac taaaacactg gctacctccc tctataacct gcttgcccgt      900

ggggccaacg tgaacttgta catgtttata ggtgggacca attttgccta ttggaatggt      960

gccaacacgc cctatgagcc acagcccacc agctatgact acgacgcccc actgagcgag     1020

gctggggacc tcactaagaa gtattttgct cttcgagaag tcattcagat gtttaaagaa     1080

gtcccagaag gccctatccc tccgtctaca cccaaatttg catatggaaa agttgctctg     1140

agaaagttca agacagtggc tgaagctctg ggtatcctgt gtcccaatgg gccagtgaaa     1200

agcctctatc ccctgacatt cactcaggta aaacagtatt ttgggtatgt gctgtaccga     1260

acaacgcttc ctcaagattg cagtaacccg aaacccattt tctcttcacc cttcaatggt     1320

gtccgtgatc gggcttacgt ctctgtggac ggggtccccc aaggaatcct tgatcgaaac     1380

ctcatgacag ctctgaacat acgggggaag gctggagcca cgctggacat cctggtggag     1440

aacatggggc gtgtgaacta tggcagattc atcaatgact tcaagggttt gatttccaac     1500

atgactatca actccactgt cctcaccaac tggacggtct tcccactgaa cactgaggcc     1560

atggtacgca accatctctg gggccgggag gccagtgatg agggtcacct tgacggacgg     1620

tcgacctcca attcttcgga cctcatactc cccacctttt acgtgggcaa cttctccatc     1680

ccctcgggca tcccagacct gccacaggac accttcatcc agtttcctgg gtggtccaag     1740

ggtcaagtat ggatcaatgg ctttaacctc ggccgatact ggcccacaat gggcccacaa     1800

aagaccttgt tcgtgccaag gaacatcctg accacttcag ccccaaacaa catcacagtg     1860

ttggagctag agtttgcacc ctgcagcgag gggaccccag agctgtgtac agtagagttt     1920

gttgacactc cggtcatttc ctga                                            1944


<210>  3
<211>  9538
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct vector for targeting exon 16 of GLB1 of Mus musculus, 
       including 5'-arm - 2A-mCherry- 3' arm

<400>  3
gagtgggagc atttgaacct tgaggacaga cctccacgtt tcccgatttg agccctccga       60

gcacccatat ttcctttagt atagtctatt acctcgttgt ctttgtgttt tttaaacagg      120

gcctcactat gctgccctgg ctgtcccaga acttgttcag gagaccaggc tgatcttgaa      180

cttaaaaatc tacctgcctc tgcctcccgt atgctgggac taaagggatg tgctaccatg      240

atcaacctat attcacgttt aaggggcatc tgaatatctt aaaacagtat tgaggggtgg      300

atatggtggc gcaagctttt aatcccagcg cttggacggc agaggcaggg agagttctgc      360

tttctgggcc agcctggtct gttgagaccc tatctcaaac aaagtaaaag acagtattga      420

aagtttaact ctagggtctg gagagatggc tcagcagtta ggtgcactgg ttgctcttcc      480

agaggaccca ggttcaattc ccagcaacca cgtggcagct caccactata actctagttc      540

cagaagatct gatgccctca cacagacaga caggcaaaac accaatgtgc atgaagtaaa      600

aataaattta aaaaagaaat aaagcttcac tgtaaattct aggaagattg gctttgtccg      660

ggtaagttaa ggcagacaca cactcactga gaagtaggtc aggccctaac aggaacttcc      720

gctttgccgt cacctctgtg ttaatagcag aaggtagaag tgtgggctgg ggtcatgatg      780

gtcagagata gtcgacttct gccatggact gtagaagttg ggactgccat gggacacccc      840

caagcccctg cagtcacctc attagtatat ccttttggtt taccaagaat aatgtaccaa      900

gaataatttc agcatatatt tttctgtttg gtctttagtg gcagggtttc acactgtagc      960

ctagactagc caaatgtgct aagcaagagt cctttactaa gctatactct caactctttc     1020

agcataaatt ttacttattt ttaaaattat ttagttactt agttcagttt ttccaaggca     1080

gcgtttctct ttgtaacacg atagccaggg ctatgtagag aaaccctgtt tcaataaata     1140

aataataagc aggttagtaa aaataaaaat aaagaaagcc tttaaaactt ctagctggaa     1200

gcctagtgtg gtggtccaca cttattaccg tagcactaca gactcagagg ctggggagtc     1260

actacaaatt caaggtcagc atgtctatat agcaagttcc aagccagctg gggctactag     1320

caaaaccaaa gaagaactaa aaccttaccc taaggccaat gaggtacttt aaccccagca     1380

cttggggagg cagaggcagg taggttgatt gatctctgag gccagcctgg tctacagagc     1440

aagttccagg acagccaggg ctatacagaa accttgtctt ggtggaaata atacatacaa     1500

acaaaaacaa ttaaaaagtt ttttaaaatg ttgttgacag cactgttatt aagaacacaa     1560

agctagaaac acctgagtac ccattttcag tagtgaagga gtgagtagcc atccgcatat     1620

ttttctaggg aatgcagtgt cgtggcagca cgcacaggcc ccgctttaat ccctaacact     1680

gaagaaaagg cgggagcagg gaacaatgta ttagtcaggc atgctggcac atgcctgtag     1740

ccccagcgta caagagtcca aagtagaggg attatgaatt tgaggttatc ctggactaac     1800

atataaaaaa aagaaaaaag tccccaaagc taattggtat atccgtacca ttgaatgtca     1860

tatgtttaaa acaataatgc atatatacac ttatttgtgc atgtatgtgt gtgagtgtat     1920

gtggtatgtg catgtacatg tgtgctatgc tcctgtgtac aggggaggag gtcagagaaa     1980

tcaaagtgtc ctgccctggc tctccacctt acccccttga gacagactct ctttctgaac     2040

ctggagctgg gctggagacc agtaagctcc cgtggtcctc tgtctcagtc tcccacaaca     2100

ctggcattgc aggcatggga aagacggcac ctagctcatt tatgtggttg ctggggactt     2160

gaacgtgtgt cctcatgctt gcccggcatg gctcttaccc actgggccat cttcccagcc     2220

tatttatgta cactggcaca taggtaaaaa ttagtcaaaa ctaattcagg gcaactgaaa     2280

tcaggagctg gtcttaagac cgtgaaaaca ggattctggc agagatgggg ctgataaagc     2340

ccaggtgaaa ctattatggt ttatgccttt caggacggag agagataaca gatcccgagt     2400

aaaaacaaga gttaaagtag ttctgagaac ctcgaaactg ccaccctttt ctaaatgact     2460

tagtccaaca gacaggcagt tctgagtcgc tgacctcctt ctgtgagcac tgaacgccta     2520

gagcttttgt ttcagtggta agtggtgccc acactgaacc aggagtacag cccatctgca     2580

ccccactcct tgcaccaggc actaggctga ccaccaaaat gtgctgtgcc cccctagcac     2640

ataattcata atcatctcct accatagcct cccaagggct aaaattacag cacccacccc     2700

attcaccctt gcttccatct tgggctgctt tggggaccgt tctcaaacta catggactct     2760

gtgttgccat cgaccctggg agggggaaaa ttaaatggag acctgcctga gttcagacag     2820

ctagcagcag gcaagaataa gatgggaaac caggaaagaa atccaggtgg actggaataa     2880

agccaaggga agttttatta tccctgcagt ctcggcactt cagaggcaga ggcaggagga     2940

ttatctggac tagatagttc aggctagtct gctgtacaga aagactgtgc aaatattaat     3000

tgtttatttt aaaatgagca ggaggggccg ggcatggtgg ccagcattcg ggaggcagag     3060

gcagatggat ttctgagttc gaggccagcc tggtctacag agtgagttcc aggacagcca     3120

gagctacaca gagaaaccct gtctcgaaaa accaaaaaaa acaaaaaaac aaaaaaaatg     3180

agcaggaggg accaccacga ggaccactct ctttccttcc ttctctcctt tcttgtcccc     3240

tctgcccttg ccctctagaa cttccctcct cttccagatc ttctgtttac ctctggcatg     3300

tgtgacccca caccaccccc agcaccccca gcagactatt ccatctgttc tgtagcccct     3360

cgaaccctga gtgcgtcttt tccagaagca gtgtccctgc tcacaggagt catccccaca     3420

gcttgcaaga ctgaaaatct tgtcttccaa gccattctcc ccacatccct cggaggaaag     3480

ctacaagaac cacacaccca acaataatgt agacactctc ctgtgtaggc ctctgctccc     3540

atccccgtct cgtcccaccg ctgtggctta cgcccactat attgcccagg ctgccttcag     3600

actcagactg atcctcctac cgtagcctct cgcatgctga ggttgtagca cctgcggcac     3660

acgccctcac attcttcccg gggttgtttg ggaaacagtt cttagactat gttcaggctt     3720

tatgcacgtc aggcgacatg gctgagctct gcctctgtgg ggcagagagc aaggaagact     3780

ctcctgctga agggtgtggg cagccatagc agagagggtt ggggtacaga caggcagttc     3840

ctgggagcca gggtggatct cagaatgtgc tgaggtgtgg cccaagactg gtctgtagct     3900

tctgaatctg aggctccaaa attcgtttct tctgcagcag gaattggtat ttggaagaag     3960

ctctaccacc taacatggga tactgggatg ctggactcta tggggcatct gttgcctctc     4020

tgatactagt gttcctcagc tgacaacctc tctctctctc tccctctctc tctctctctt     4080

cctctctctc cctctctctc tctctctctc tctctctctc tagggtcaag tatggatcaa     4140

tggctttaac ctcggccgat actggcccac aatgggccca caaaagacct tgttcgtgcc     4200

aaggaacatc ctgaccactt cagccccaaa caacatcaca gtgttggagc tagagtttgc     4260

accctgcagc gaggggaccc cagagctgtg tacagtagag tttgttgaca ctccggtcat     4320

ttccggtagc ggagctacta acttcagcct gctgaagcag gctggcgacg tggaggagaa     4380

ccctggtcct atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat     4440

gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga     4500

gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg     4560

ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta     4620

cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa     4680

gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc     4740

cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga     4800

cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc     4860

cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca     4920

ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc     4980

ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga     5040

acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta     5100

actgtgagca ctgaacgcct actcgagccc tcagtctcct gtcaaggact tgaccatcgg     5160

tggccatttt ccaagccagt ctggtcaaga ctcatggctg aacctctgag actgagcctt     5220

ggggagcaca gctctactct ggttacacgg atcacctttg ttgtgctaga atggaagcta     5280

catttctgga atgtgtgtac cctgctggtg actttgcagc cccgccttgc ctgacctccc     5340

accctagtgg ccaccgggaa ggctgaaggg agtggacacc acaggaagac acaggttgga     5400

gcaaaacttg aataatgtcc tttatcctga tttgaaataa tcacgtcatc tttctgctga     5460

ataaaattgg tttgcaagtt gtgggggtcc tgtcattaat atatcaggca ggtattaaac     5520

tccagggtct gacttaaatc tcaactagac ctgagtgggc tgcctgaagc tacttcacca     5580

gcttgaggta caaagtgggt agaagagaga gctggtttgg gtgagaagag ggctttgttt     5640

ttgatcctgc tttgactatt tctgaatctc tgtgatatct gcttggtgag agatctccag     5700

ttataaacca tctaaaagat taatagttgc tcaaagtagg tagtatggtc tctctataca     5760

attgaataga attctctctc tctctctctc tctctctctc tctctctctc tctctctcct     5820

ctctctctct gtgtgtgtgt gtgtgttcat gttcatgtat gtatgaagtt gcatgtatat     5880

gcatgtatgt atgcaggtca gaggcccatc ttgggtgtca tttctcagga atgtcttagt     5940

atctaggcac atacaggatg tataaaatgt aattctgctt atgtttgaga ccctgtctcc     6000

aaaaaaaaaa agtgttaacg tggggctggt ctgtagctaa gttgataaaa cacgtttgtt     6060

tgtcatgcaa gaagcactgg gttctctccc tagcagtgca caaaaccagg catgatagta     6120

catgttgtgg ttataaaaag ataaagaggt ctaagaacat gttctatggg ggtgtggtga     6180

ggtgtgtgtg tgggtgcctc ggcaggccca tgccaagaca tcccttccct ctaaaggact     6240

agccccccca cacacacaca cacacaccac gacagtatag tatagaatag agtttattta     6300

gggcatgggg aggggagcta agatggtagt agagacagag agagggagag agtagagaag     6360

tagaggctgg ccaggacaaa gtgaagagag ggggaaaggg gatggggaga gggggaacaa     6420

gggagcaaga ggcaagagag taagagagag aggagggggc aagcagccta cctgggtttt     6480

gctaggtaac tgtgggatgg agtttagaca gaatgctaac agtacacaca cgtgtaatag     6540

cattcaagaa gtgagaggag agcactgaag tccaagttta tcctcagcta cacagcgggg     6600

tccgtacctt gtgttatatg taaccctgtc tcaaaaaaat aataatatga ttgcatctga     6660

gtggtaaaca tttgggtact tggctacatt tcctaaccta tactttaaat ttggaggaag     6720

ggaacaagtg tgcgcacaca ctagaagttt ccagccccac ccccaccccc gtctcccact     6780

cccacgggag cactgggatt acacttgtgc acactattgt tttaagcttt tacatggcta     6840

ccagaggtcc aagctcaagt cctcatgctt gcttgggaag tgccgtcaac caccgagcca     6900

cctccacatc tggcttttgt ttcagttttt atgtatatga gtactgtatt tgcatgtctg     6960

cctgtatgat gaaagagggc atcagatccc aatatggttg tgagccacca tgtggggtct     7020

ggggattgaa ctctggacct ctggaagagc agctggtgct cttaacccct gagccaacct     7080

ccagccctgt tttgtttttt tgaaacagtt ccagagctgc ctttgagctc atcatgtagc     7140

caaggatagc ctttaatttc tgatccttct tacaaacatg caccgcacgc ctttaatccc     7200

agcactcggg aggcagaggc aggcagattt ctgagttcga ggccagcctg gtctacaaag     7260

tgagttccag gacagccagg gctatacaga ggaagcctgt ctcgaaaaac aaaacaaaaa     7320

caaaaaaaaa aaaaaaaaag cttttccatt cttagactct gatgggaagg gagcccaggg     7380

cttcatgcat gttggacatt tgactgacag agctacatcc ccatccccag gcctttactc     7440

cattgtctaa tgtcactttc tgtttgtcag tctgtccata tttgtttctc ggtaattcta     7500

gtttacttga aatgaaatcg cttagccctc agtctcctgt caagtgactg ggacagcaga     7560

tgatctccca tgctctttat ttctttcttc ttatgaaaaa ataaaaggtc attttctgaa     7620

atcatttctt agtcactgtg agggatctaa gctgtggtac aataccacat gtgggctata     7680

ggaacgtaaa gtgactgtcc agagagtggg aggggacacg ctggctctag gcaactgtgt     7740

tccagcccct gagtgtcaga gcttcctctc agcttccctt gcccctctgg ctgactggaa     7800

acttgcactg aggtttctac actttgattt ttcaagacag ggtttctctg tgtagccctg     7860

gctgttctgg cactcactct gtagaccagg ctggcctcag attcacagag accgcttgcc     7920

tctgcctcct gagcaccagg atcaaaggcg tgtgccacca ctgcctacca catggctcag     7980

ttgatgaagt gtttgcctgg caagagtgag gccctgggtt ccaccccccc tcactgaaga     8040

aacaaggtat ggccaagcac atgggtaatc ctagcactct aaagtggagg ctggaagctc     8100

acgatcagag caagttccag gtcagaatac aagtaacagt actgctaata gttcggactt     8160

tgtgatggaa actcaagtta gtctctccct cgccctctct ctctcatcat tgttggcaag     8220

aaagtgactt gctcatacgc cttggtttcc tcaagtgtgg ctcccacttg ggctttctca     8280

gttgcgtcaa gcctagaagt atcctggcta gcacctgcca cggagcatgc tctagtttcg     8340

tttctgctct tgtgtgagca ccctggccaa aaaaaacagc ttcggggaga aaggacttgt     8400

tttaactcac aatttcgcca ggcaatgatg gcacatgcct ttaaccccag aacttgggag     8460

gcagaagcag gtggatttct gagttcgagg ccagcctggt ctacagaatg agttccagga     8520

cagccagggc tgtacagaga aaccctgtct cgaaacaaaa caaaacaaac aaacaaacaa     8580

acaaacaaaa aaaaaccctc acgattttac tggtgaacgt ggcaggagcc ggaagctagt     8640

cagcggtatt cgatggtcac aagcagataa gtgataaggg cacaagagct cgcttgcttg     8700

ctgacttatg cttagctcaa gcttgactct ctgtgctgtt tgaggccccc tgcccaggga     8760

atggtgccac ccacagtggg ctgtgtctcc ccatatcaac tacttaagac agtccaccat     8820

agacacacca aggctaacag cgtagactcc tcctcactaa gacatccctc caatggttct     8880

actctgggtc tagttgacaa agctaaacac cacacacata ttcattgaca ctttcgatca     8940

agtccccacc ctcgcagaga agccaaaggg gtggggaaag tgatgccacc caagatggtt     9000

ctctgtagcc ctctgtgttc agttcagcca ctcggtgcta ctctgccact gtgctagact     9060

caggctacta agcaaataaa cttgtctgtg atgagccgtg ctacacacta aggactagag     9120

gctgagggaa tggcagcatc caaagggatc ctctagccat gacctgggag ccaccgtgga     9180

cactcagcct gtagaaaagg ctacaggccc ccaatggcgt aagcctaaga ggatggactg     9240

gccccaaccc tcacctggag aacgcaggag agctggccct ggtggcatgg gccctggaga     9300

gctggtgagc tgacccaatc agtccagatc caggtctttg agttggccca acccaacatc     9360

tacccatcta tgtatgagct gctggagaga aggaaggggc cagtcctgca gatggaaaac     9420

tgtggggtct ccatgacaca ggacagcagc aggatagcca agaggtgtcc cagtgaggag     9480

ccacgattga tggtgcagca gaagtcagag gcctcaaacc agctcgatga ctccttgc       9538


<210>  4
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  2A sequence

<400>  4
ggtagcggag ctactaactt cagcctgctg aagcaggctg gcgacgtgga ggagaaccct       60

ggtcct                                                                  66


<210>  5
<211>  711
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mCherry sequence

<400>  5
atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag       60

gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc      120

cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc      180

ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac      240

cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc      300

gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac      360

ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta      420

atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc      480

gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct      540

gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc      600

aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa      660

cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta a               711


<210>  6
<211>  4324
<212>  DNA
<213>  Mus musculus

<400>  6
gagtgggagc atttgaacct tgaggacaga cctccacgtt tcccgatttg agccctccga       60

gcacccatat ttcctttagt atagtctatt acctcgttgt ctttgtgttt tttaaacagg      120

gcctcactat gctgccctgg ctgtcccaga acttgttcag gagaccaggc tgatcttgaa      180

cttaaaaatc tacctgcctc tgcctcccgt atgctgggac taaagggatg tgctaccatg      240

atcaacctat attcacgttt aaggggcatc tgaatatctt aaaacagtat tgaggggtgg      300

atatggtggc gcaagctttt aatcccagcg cttggacggc agaggcaggg agagttctgc      360

tttctgggcc agcctggtct gttgagaccc tatctcaaac aaagtaaaag acagtattga      420

aagtttaact ctagggtctg gagagatggc tcagcagtta ggtgcactgg ttgctcttcc      480

agaggaccca ggttcaattc ccagcaacca cgtggcagct caccactata actctagttc      540

cagaagatct gatgccctca cacagacaga caggcaaaac accaatgtgc atgaagtaaa      600

aataaattta aaaaagaaat aaagcttcac tgtaaattct aggaagattg gctttgtccg      660

ggtaagttaa ggcagacaca cactcactga gaagtaggtc aggccctaac aggaacttcc      720

gctttgccgt cacctctgtg ttaatagcag aaggtagaag tgtgggctgg ggtcatgatg      780

gtcagagata gtcgacttct gccatggact gtagaagttg ggactgccat gggacacccc      840

caagcccctg cagtcacctc attagtatat ccttttggtt taccaagaat aatgtaccaa      900

gaataatttc agcatatatt tttctgtttg gtctttagtg gcagggtttc acactgtagc      960

ctagactagc caaatgtgct aagcaagagt cctttactaa gctatactct caactctttc     1020

agcataaatt ttacttattt ttaaaattat ttagttactt agttcagttt ttccaaggca     1080

gcgtttctct ttgtaacacg atagccaggg ctatgtagag aaaccctgtt tcaataaata     1140

aataataagc aggttagtaa aaataaaaat aaagaaagcc tttaaaactt ctagctggaa     1200

gcctagtgtg gtggtccaca cttattaccg tagcactaca gactcagagg ctggggagtc     1260

actacaaatt caaggtcagc atgtctatat agcaagttcc aagccagctg gggctactag     1320

caaaaccaaa gaagaactaa aaccttaccc taaggccaat gaggtacttt aaccccagca     1380

cttggggagg cagaggcagg taggttgatt gatctctgag gccagcctgg tctacagagc     1440

aagttccagg acagccaggg ctatacagaa accttgtctt ggtggaaata atacatacaa     1500

acaaaaacaa ttaaaaagtt ttttaaaatg ttgttgacag cactgttatt aagaacacaa     1560

agctagaaac acctgagtac ccattttcag tagtgaagga gtgagtagcc atccgcatat     1620

ttttctaggg aatgcagtgt cgtggcagca cgcacaggcc ccgctttaat ccctaacact     1680

gaagaaaagg cgggagcagg gaacaatgta ttagtcaggc atgctggcac atgcctgtag     1740

ccccagcgta caagagtcca aagtagaggg attatgaatt tgaggttatc ctggactaac     1800

atataaaaaa aagaaaaaag tccccaaagc taattggtat atccgtacca ttgaatgtca     1860

tatgtttaaa acaataatgc atatatacac ttatttgtgc atgtatgtgt gtgagtgtat     1920

gtggtatgtg catgtacatg tgtgctatgc tcctgtgtac aggggaggag gtcagagaaa     1980

tcaaagtgtc ctgccctggc tctccacctt acccccttga gacagactct ctttctgaac     2040

ctggagctgg gctggagacc agtaagctcc cgtggtcctc tgtctcagtc tcccacaaca     2100

ctggcattgc aggcatggga aagacggcac ctagctcatt tatgtggttg ctggggactt     2160

gaacgtgtgt cctcatgctt gcccggcatg gctcttaccc actgggccat cttcccagcc     2220

tatttatgta cactggcaca taggtaaaaa ttagtcaaaa ctaattcagg gcaactgaaa     2280

tcaggagctg gtcttaagac cgtgaaaaca ggattctggc agagatgggg ctgataaagc     2340

ccaggtgaaa ctattatggt ttatgccttt caggacggag agagataaca gatcccgagt     2400

aaaaacaaga gttaaagtag ttctgagaac ctcgaaactg ccaccctttt ctaaatgact     2460

tagtccaaca gacaggcagt tctgagtcgc tgacctcctt ctgtgagcac tgaacgccta     2520

gagcttttgt ttcagtggta agtggtgccc acactgaacc aggagtacag cccatctgca     2580

ccccactcct tgcaccaggc actaggctga ccaccaaaat gtgctgtgcc cccctagcac     2640

ataattcata atcatctcct accatagcct cccaagggct aaaattacag cacccacccc     2700

attcaccctt gcttccatct tgggctgctt tggggaccgt tctcaaacta catggactct     2760

gtgttgccat cgaccctggg agggggaaaa ttaaatggag acctgcctga gttcagacag     2820

ctagcagcag gcaagaataa gatgggaaac caggaaagaa atccaggtgg actggaataa     2880

agccaaggga agttttatta tccctgcagt ctcggcactt cagaggcaga ggcaggagga     2940

ttatctggac tagatagttc aggctagtct gctgtacaga aagactgtgc aaatattaat     3000

tgtttatttt aaaatgagca ggaggggccg ggcatggtgg ccagcattcg ggaggcagag     3060

gcagatggat ttctgagttc gaggccagcc tggtctacag agtgagttcc aggacagcca     3120

gagctacaca gagaaaccct gtctcgaaaa accaaaaaaa acaaaaaaac aaaaaaaatg     3180

agcaggaggg accaccacga ggaccactct ctttccttcc ttctctcctt tcttgtcccc     3240

tctgcccttg ccctctagaa cttccctcct cttccagatc ttctgtttac ctctggcatg     3300

tgtgacccca caccaccccc agcaccccca gcagactatt ccatctgttc tgtagcccct     3360

cgaaccctga gtgcgtcttt tccagaagca gtgtccctgc tcacaggagt catccccaca     3420

gcttgcaaga ctgaaaatct tgtcttccaa gccattctcc ccacatccct cggaggaaag     3480

ctacaagaac cacacaccca acaataatgt agacactctc ctgtgtaggc ctctgctccc     3540

atccccgtct cgtcccaccg ctgtggctta cgcccactat attgcccagg ctgccttcag     3600

actcagactg atcctcctac cgtagcctct cgcatgctga ggttgtagca cctgcggcac     3660

acgccctcac attcttcccg gggttgtttg ggaaacagtt cttagactat gttcaggctt     3720

tatgcacgtc aggcgacatg gctgagctct gcctctgtgg ggcagagagc aaggaagact     3780

ctcctgctga agggtgtggg cagccatagc agagagggtt ggggtacaga caggcagttc     3840

ctgggagcca gggtggatct cagaatgtgc tgaggtgtgg cccaagactg gtctgtagct     3900

tctgaatctg aggctccaaa attcgtttct tctgcagcag gaattggtat ttggaagaag     3960

ctctaccacc taacatggga tactgggatg ctggactcta tggggcatct gttgcctctc     4020

tgatactagt gttcctcagc tgacaacctc tctctctctc tccctctctc tctctctctt     4080

cctctctctc cctctctctc tctctctctc tctctctctc tagggtcaag tatggatcaa     4140

tggctttaac ctcggccgat actggcccac aatgggccca caaaagacct tgttcgtgcc     4200

aaggaacatc ctgaccactt cagccccaaa caacatcaca gtgttggagc tagagtttgc     4260

accctgcagc gaggggaccc cagagctgtg tacagtagag tttgttgaca ctccggtcat     4320

ttcc                                                                  4324


<210>  7
<211>  4392
<212>  DNA
<213>  Mus musculus

<400>  7
gacttgacca tcggtggcca ttttccaagc cagtctggtc aagactcatg gctgaacctc       60

tgagactgag ccttggggag cacagctcta ctctggttac acggatcacc tttgttgtgc      120

tagaatggaa gctacatttc tggaatgtgt gtaccctgct ggtgactttg cagccccgcc      180

ttgcctgacc tcccacccta gtggccaccg ggaaggctga agggagtgga caccacagga      240

agacacaggt tggagcaaaa cttgaataat gtcctttatc ctgatttgaa ataatcacgt      300

catctttctg ctgaataaaa ttggtttgca agttgtgggg gtcctgtcat taatatatca      360

ggcaggtatt aaactccagg gtctgactta aatctcaact agacctgagt gggctgcctg      420

aagctacttc accagcttga ggtacaaagt gggtagaaga gagagctggt ttgggtgaga      480

agagggcttt gtttttgatc ctgctttgac tatttctgaa tctctgtgat atctgcttgg      540

tgagagatct ccagttataa accatctaaa agattaatag ttgctcaaag taggtagtat      600

ggtctctcta tacaattgaa tagaattctc tctctctctc tctctctctc tctctctctc      660

tctctctctc tcctctctct ctctgtgtgt gtgtgtgtgt tcatgttcat gtatgtatga      720

agttgcatgt atatgcatgt atgtatgcag gtcagaggcc catcttgggt gtcatttctc      780

aggaatgtct tagtatctag gcacatacag gatgtataaa atgtaattct gcttatgttt      840

gagaccctgt ctccaaaaaa aaaaagtgtt aacgtggggc tggtctgtag ctaagttgat      900

aaaacacgtt tgtttgtcat gcaagaagca ctgggttctc tccctagcag tgcacaaaac      960

caggcatgat agtacatgtt gtggttataa aaagataaag aggtctaaga acatgttcta     1020

tgggggtgtg gtgaggtgtg tgtgtgggtg cctcggcagg cccatgccaa gacatccctt     1080

ccctctaaag gactagcccc cccacacaca cacacacaca ccacgacagt atagtataga     1140

atagagttta tttagggcat ggggagggga gctaagatgg tagtagagac agagagaggg     1200

agagagtaga gaagtagagg ctggccagga caaagtgaag agagggggaa aggggatggg     1260

gagaggggga acaagggagc aagaggcaag agagtaagag agagaggagg gggcaagcag     1320

cctacctggg ttttgctagg taactgtggg atggagttta gacagaatgc taacagtaca     1380

cacacgtgta atagcattca agaagtgaga ggagagcact gaagtccaag tttatcctca     1440

gctacacagc ggggtccgta ccttgtgtta tatgtaaccc tgtctcaaaa aaataataat     1500

atgattgcat ctgagtggta aacatttggg tacttggcta catttcctaa cctatacttt     1560

aaatttggag gaagggaaca agtgtgcgca cacactagaa gtttccagcc ccacccccac     1620

ccccgtctcc cactcccacg ggagcactgg gattacactt gtgcacacta ttgttttaag     1680

cttttacatg gctaccagag gtccaagctc aagtcctcat gcttgcttgg gaagtgccgt     1740

caaccaccga gccacctcca catctggctt ttgtttcagt ttttatgtat atgagtactg     1800

tatttgcatg tctgcctgta tgatgaaaga gggcatcaga tcccaatatg gttgtgagcc     1860

accatgtggg gtctggggat tgaactctgg acctctggaa gagcagctgg tgctcttaac     1920

ccctgagcca acctccagcc ctgttttgtt tttttgaaac agttccagag ctgcctttga     1980

gctcatcatg tagccaagga tagcctttaa tttctgatcc ttcttacaaa catgcaccgc     2040

acgcctttaa tcccagcact cgggaggcag aggcaggcag atttctgagt tcgaggccag     2100

cctggtctac aaagtgagtt ccaggacagc cagggctata cagaggaagc ctgtctcgaa     2160

aaacaaaaca aaaacaaaaa aaaaaaaaaa aaagcttttc cattcttaga ctctgatggg     2220

aagggagccc agggcttcat gcatgttgga catttgactg acagagctac atccccatcc     2280

ccaggccttt actccattgt ctaatgtcac tttctgtttg tcagtctgtc catatttgtt     2340

tctcggtaat tctagtttac ttgaaatgaa atcgcttagc cctcagtctc ctgtcaagtg     2400

actgggacag cagatgatct cccatgctct ttatttcttt cttcttatga aaaaataaaa     2460

ggtcattttc tgaaatcatt tcttagtcac tgtgagggat ctaagctgtg gtacaatacc     2520

acatgtgggc tataggaacg taaagtgact gtccagagag tgggagggga cacgctggct     2580

ctaggcaact gtgttccagc ccctgagtgt cagagcttcc tctcagcttc ccttgcccct     2640

ctggctgact ggaaacttgc actgaggttt ctacactttg atttttcaag acagggtttc     2700

tctgtgtagc cctggctgtt ctggcactca ctctgtagac caggctggcc tcagattcac     2760

agagaccgct tgcctctgcc tcctgagcac caggatcaaa ggcgtgtgcc accactgcct     2820

accacatggc tcagttgatg aagtgtttgc ctggcaagag tgaggccctg ggttccaccc     2880

cccctcactg aagaaacaag gtatggccaa gcacatgggt aatcctagca ctctaaagtg     2940

gaggctggaa gctcacgatc agagcaagtt ccaggtcaga atacaagtaa cagtactgct     3000

aatagttcgg actttgtgat ggaaactcaa gttagtctct ccctcgccct ctctctctca     3060

tcattgttgg caagaaagtg acttgctcat acgccttggt ttcctcaagt gtggctccca     3120

cttgggcttt ctcagttgcg tcaagcctag aagtatcctg gctagcacct gccacggagc     3180

atgctctagt ttcgtttctg ctcttgtgtg agcaccctgg ccaaaaaaaa cagcttcggg     3240

gagaaaggac ttgttttaac tcacaatttc gccaggcaat gatggcacat gcctttaacc     3300

ccagaacttg ggaggcagaa gcaggtggat ttctgagttc gaggccagcc tggtctacag     3360

aatgagttcc aggacagcca gggctgtaca gagaaaccct gtctcgaaac aaaacaaaac     3420

aaacaaacaa acaaacaaac aaaaaaaaac cctcacgatt ttactggtga acgtggcagg     3480

agccggaagc tagtcagcgg tattcgatgg tcacaagcag ataagtgata agggcacaag     3540

agctcgcttg cttgctgact tatgcttagc tcaagcttga ctctctgtgc tgtttgaggc     3600

cccctgccca gggaatggtg ccacccacag tgggctgtgt ctccccatat caactactta     3660

agacagtcca ccatagacac accaaggcta acagcgtaga ctcctcctca ctaagacatc     3720

cctccaatgg ttctactctg ggtctagttg acaaagctaa acaccacaca catattcatt     3780

gacactttcg atcaagtccc caccctcgca gagaagccaa aggggtgggg aaagtgatgc     3840

cacccaagat ggttctctgt agccctctgt gttcagttca gccactcggt gctactctgc     3900

cactgtgcta gactcaggct actaagcaaa taaacttgtc tgtgatgagc cgtgctacac     3960

actaaggact agaggctgag ggaatggcag catccaaagg gatcctctag ccatgacctg     4020

ggagccaccg tggacactca gcctgtagaa aaggctacag gcccccaatg gcgtaagcct     4080

aagaggatgg actggcccca accctcacct ggagaacgca ggagagctgg ccctggtggc     4140

atgggccctg gagagctggt gagctgaccc aatcagtcca gatccaggtc tttgagttgg     4200

cccaacccaa catctaccca tctatgtatg agctgctgga gagaaggaag gggccagtcc     4260

tgcagatgga aaactgtggg gtctccatga cacaggacag cagcaggata gccaagaggt     4320

gtcccagtga ggagccacga ttgatggtgc agcagaagtc agaggcctca aaccagctcg     4380

atgactcctt gc                                                         4392


<210>  8
<211>  128
<212>  DNA
<213>  Mus musculus

<400>  8
gaccccagag ctgtgtacag tagagtttgt tgacactccg gtcatttcct gacctgactt       60

gaccatcggt ggccattttc caagccagtc tggtcaagac tcatggctga acctctgaga      120

ctgagcct                                                               128


<210>  9
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' guide RNA1 for knock-in site of exon 16 of Glb1 gene of Mus 
       musculus

<400>  9
tggccaccga tggtcaagtc agg                                               23


<210>  10
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' guide RNA1 for knock-in site of exon 16 of Glb1 gene  of Mus 
       musculus

<400>  10
accgatggtc aagtcaggtc agg                                               23


