                               SEQUENCE LISTING

<110> JOSLIN DIABETES CENTER
 
<120> METHODS OF DIAGNOSING AND PREDICTING RENAL DECLINE

<130> J103021 1090WO

<140>
<141>

<150> 63/215,150
<151> 2021-06-25

<150> 63/172,541
<151> 2021-04-08

<160> 11    

<170> PatentIn version 3.5

<210> 1
<211> 303
<212> PRT
<213> Homo sapiens

<400> 1
Met Arg Ala Trp Ile Phe Phe Leu Leu Cys Leu Ala Gly Arg Ala Leu 
1               5                   10                  15      


Ala Ala Pro Gln Gln Glu Ala Leu Pro Asp Glu Thr Glu Val Val Glu 
            20                  25                  30          


Glu Thr Val Ala Glu Val Thr Glu Val Ser Val Gly Ala Asn Pro Val 
        35                  40                  45              


Gln Val Glu Val Gly Glu Phe Asp Asp Gly Ala Glu Glu Thr Glu Glu 
    50                  55                  60                  


Glu Val Val Ala Glu Asn Pro Cys Gln Asn His His Cys Lys His Gly 
65                  70                  75                  80  


Lys Val Cys Glu Leu Asp Glu Asn Asn Thr Pro Met Cys Val Cys Gln 
                85                  90                  95      


Asp Pro Thr Ser Cys Pro Ala Pro Ile Gly Glu Phe Glu Lys Val Cys 
            100                 105                 110         


Ser Asn Asp Asn Lys Thr Phe Asp Ser Ser Cys His Phe Phe Ala Thr 
        115                 120                 125             


Lys Cys Thr Leu Glu Gly Thr Lys Lys Gly His Lys Leu His Leu Asp 
    130                 135                 140                 


Tyr Ile Gly Pro Cys Lys Tyr Ile Pro Pro Cys Leu Asp Ser Glu Leu 
145                 150                 155                 160 


Thr Glu Phe Pro Leu Arg Met Arg Asp Trp Leu Lys Asn Val Leu Val 
                165                 170                 175     


Thr Leu Tyr Glu Arg Asp Glu Asp Asn Asn Leu Leu Thr Glu Lys Gln 
            180                 185                 190         


Lys Leu Arg Val Lys Lys Ile His Glu Asn Glu Lys Arg Leu Glu Ala 
        195                 200                 205             


Gly Asp His Pro Val Glu Leu Leu Ala Arg Asp Phe Glu Lys Asn Tyr 
    210                 215                 220                 


Asn Met Tyr Ile Phe Pro Val His Trp Gln Phe Gly Gln Leu Asp Gln 
225                 230                 235                 240 


His Pro Ile Asp Gly Tyr Leu Ser His Thr Glu Leu Ala Pro Leu Arg 
                245                 250                 255     


Ala Pro Leu Ile Pro Met Glu His Cys Thr Thr Arg Phe Phe Glu Thr 
            260                 265                 270         


Cys Asp Leu Asp Asn Asp Lys Tyr Ile Ala Leu Asp Glu Trp Ala Gly 
        275                 280                 285             


Cys Phe Gly Ile Lys Gln Lys Asp Ile Asp Lys Asp Leu Val Ile 
    290                 295                 300             


<210> 2
<211> 91
<212> PRT
<213> Homo sapiens

<400> 2
Met Lys Val Ser Ala Ala Ala Leu Ala Val Ile Leu Ile Ala Thr Ala 
1               5                   10                  15      


Leu Cys Ala Pro Ala Ser Ala Ser Pro Tyr Ser Ser Asp Thr Thr Pro 
            20                  25                  30          


Cys Cys Phe Ala Tyr Ile Ala Arg Pro Leu Pro Arg Ala His Ile Lys 
        35                  40                  45              


Glu Tyr Phe Tyr Thr Ser Gly Lys Cys Ser Asn Pro Ala Val Val Phe 
    50                  55                  60                  


Val Thr Arg Lys Asn Arg Gln Val Cys Ala Asn Pro Glu Lys Lys Trp 
65                  70                  75                  80  


Val Arg Glu Tyr Ile Asn Ser Leu Glu Met Ser 
                85                  90      


<210> 3
<211> 770
<212> PRT
<213> Homo sapiens

<400> 3
Met Leu Pro Gly Leu Ala Leu Leu Leu Leu Ala Ala Trp Thr Ala Arg 
1               5                   10                  15      


Ala Leu Glu Val Pro Thr Asp Gly Asn Ala Gly Leu Leu Ala Glu Pro 
            20                  25                  30          


Gln Ile Ala Met Phe Cys Gly Arg Leu Asn Met His Met Asn Val Gln 
        35                  40                  45              


Asn Gly Lys Trp Asp Ser Asp Pro Ser Gly Thr Lys Thr Cys Ile Asp 
    50                  55                  60                  


Thr Lys Glu Gly Ile Leu Gln Tyr Cys Gln Glu Val Tyr Pro Glu Leu 
65                  70                  75                  80  


Gln Ile Thr Asn Val Val Glu Ala Asn Gln Pro Val Thr Ile Gln Asn 
                85                  90                  95      


Trp Cys Lys Arg Gly Arg Lys Gln Cys Lys Thr His Pro His Phe Val 
            100                 105                 110         


Ile Pro Tyr Arg Cys Leu Val Gly Glu Phe Val Ser Asp Ala Leu Leu 
        115                 120                 125             


Val Pro Asp Lys Cys Lys Phe Leu His Gln Glu Arg Met Asp Val Cys 
    130                 135                 140                 


Glu Thr His Leu His Trp His Thr Val Ala Lys Glu Thr Cys Ser Glu 
145                 150                 155                 160 


Lys Ser Thr Asn Leu His Asp Tyr Gly Met Leu Leu Pro Cys Gly Ile 
                165                 170                 175     


Asp Lys Phe Arg Gly Val Glu Phe Val Cys Cys Pro Leu Ala Glu Glu 
            180                 185                 190         


Ser Asp Asn Val Asp Ser Ala Asp Ala Glu Glu Asp Asp Ser Asp Val 
        195                 200                 205             


Trp Trp Gly Gly Ala Asp Thr Asp Tyr Ala Asp Gly Ser Glu Asp Lys 
    210                 215                 220                 


Val Val Glu Val Ala Glu Glu Glu Glu Val Ala Glu Val Glu Glu Glu 
225                 230                 235                 240 


Glu Ala Asp Asp Asp Glu Asp Asp Glu Asp Gly Asp Glu Val Glu Glu 
                245                 250                 255     


Glu Ala Glu Glu Pro Tyr Glu Glu Ala Thr Glu Arg Thr Thr Ser Ile 
            260                 265                 270         


Ala Thr Thr Thr Thr Thr Thr Thr Glu Ser Val Glu Glu Val Val Arg 
        275                 280                 285             


Glu Val Cys Ser Glu Gln Ala Glu Thr Gly Pro Cys Arg Ala Met Ile 
    290                 295                 300                 


Ser Arg Trp Tyr Phe Asp Val Thr Glu Gly Lys Cys Ala Pro Phe Phe 
305                 310                 315                 320 


Tyr Gly Gly Cys Gly Gly Asn Arg Asn Asn Phe Asp Thr Glu Glu Tyr 
                325                 330                 335     


Cys Met Ala Val Cys Gly Ser Ala Met Ser Gln Ser Leu Leu Lys Thr 
            340                 345                 350         


Thr Gln Glu Pro Leu Ala Arg Asp Pro Val Lys Leu Pro Thr Thr Ala 
        355                 360                 365             


Ala Ser Thr Pro Asp Ala Val Asp Lys Tyr Leu Glu Thr Pro Gly Asp 
    370                 375                 380                 


Glu Asn Glu His Ala His Phe Gln Lys Ala Lys Glu Arg Leu Glu Ala 
385                 390                 395                 400 


Lys His Arg Glu Arg Met Ser Gln Val Met Arg Glu Trp Glu Glu Ala 
                405                 410                 415     


Glu Arg Gln Ala Lys Asn Leu Pro Lys Ala Asp Lys Lys Ala Val Ile 
            420                 425                 430         


Gln His Phe Gln Glu Lys Val Glu Ser Leu Glu Gln Glu Ala Ala Asn 
        435                 440                 445             


Glu Arg Gln Gln Leu Val Glu Thr His Met Ala Arg Val Glu Ala Met 
    450                 455                 460                 


Leu Asn Asp Arg Arg Arg Leu Ala Leu Glu Asn Tyr Ile Thr Ala Leu 
465                 470                 475                 480 


Gln Ala Val Pro Pro Arg Pro Arg His Val Phe Asn Met Leu Lys Lys 
                485                 490                 495     


Tyr Val Arg Ala Glu Gln Lys Asp Arg Gln His Thr Leu Lys His Phe 
            500                 505                 510         


Glu His Val Arg Met Val Asp Pro Lys Lys Ala Ala Gln Ile Arg Ser 
        515                 520                 525             


Gln Val Met Thr His Leu Arg Val Ile Tyr Glu Arg Met Asn Gln Ser 
    530                 535                 540                 


Leu Ser Leu Leu Tyr Asn Val Pro Ala Val Ala Glu Glu Ile Gln Asp 
545                 550                 555                 560 


Glu Val Asp Glu Leu Leu Gln Lys Glu Gln Asn Tyr Ser Asp Asp Val 
                565                 570                 575     


Leu Ala Asn Met Ile Ser Glu Pro Arg Ile Ser Tyr Gly Asn Asp Ala 
            580                 585                 590         


Leu Met Pro Ser Leu Thr Glu Thr Lys Thr Thr Val Glu Leu Leu Pro 
        595                 600                 605             


Val Asn Gly Glu Phe Ser Leu Asp Asp Leu Gln Pro Trp His Ser Phe 
    610                 615                 620                 


Gly Ala Asp Ser Val Pro Ala Asn Thr Glu Asn Glu Val Glu Pro Val 
625                 630                 635                 640 


Asp Ala Arg Pro Ala Ala Asp Arg Gly Leu Thr Thr Arg Pro Gly Ser 
                645                 650                 655     


Gly Leu Thr Asn Ile Lys Thr Glu Glu Ile Ser Glu Val Lys Met Asp 
            660                 665                 670         


Ala Glu Phe Arg His Asp Ser Gly Tyr Glu Val His His Gln Lys Leu 
        675                 680                 685             


Val Phe Phe Ala Glu Asp Val Gly Ser Asn Lys Gly Ala Ile Ile Gly 
    690                 695                 700                 


Leu Met Val Gly Gly Val Val Ile Ala Thr Val Ile Val Ile Thr Leu 
705                 710                 715                 720 


Val Met Leu Lys Lys Lys Gln Tyr Thr Ser Ile His His Gly Val Val 
                725                 730                 735     


Glu Val Asp Ala Ala Val Thr Pro Glu Glu Arg His Leu Ser Lys Met 
            740                 745                 750         


Gln Gln Asn Gly Tyr Glu Asn Pro Thr Tyr Lys Phe Phe Glu Gln Met 
        755                 760                 765             


Gln Asn 
    770 


<210> 4
<211> 101
<212> PRT
<213> Homo sapiens

<400> 4
Met Ser Ser Ala Ala Gly Phe Cys Ala Ser Arg Pro Gly Leu Leu Phe 
1               5                   10                  15      


Leu Gly Leu Leu Leu Leu Pro Leu Val Val Ala Phe Ala Ser Ala Glu 
            20                  25                  30          


Ala Glu Glu Asp Gly Asp Leu Gln Cys Leu Cys Val Lys Thr Thr Ser 
        35                  40                  45              


Gln Val Arg Pro Arg His Ile Thr Ser Leu Glu Val Ile Lys Ala Gly 
    50                  55                  60                  


Pro His Cys Pro Thr Ala Gln Leu Ile Ala Thr Leu Lys Asn Gly Arg 
65                  70                  75                  80  


Lys Ile Cys Leu Asp Leu Gln Ala Pro Leu Tyr Lys Lys Ile Ile Lys 
                85                  90                  95      


Lys Leu Leu Glu Ser 
            100     


<210> 5
<211> 116
<212> PRT
<213> Homo sapiens

<400> 5
Met Ala Ser Thr Val Val Ala Val Gly Leu Thr Ile Ala Ala Ala Gly 
1               5                   10                  15      


Phe Ala Gly Arg Tyr Val Leu Gln Ala Met Lys His Met Glu Pro Gln 
            20                  25                  30          


Val Lys Gln Val Phe Gln Ser Leu Pro Lys Ser Ala Phe Ser Gly Gly 
        35                  40                  45              


Tyr Tyr Arg Gly Gly Phe Glu Pro Lys Met Thr Lys Arg Glu Ala Ala 
    50                  55                  60                  


Leu Ile Leu Gly Val Ser Pro Thr Ala Asn Lys Gly Lys Ile Arg Asp 
65                  70                  75                  80  


Ala His Arg Arg Ile Met Leu Leu Asn His Pro Asp Lys Gly Gly Ser 
                85                  90                  95      


Pro Tyr Ile Ala Ala Lys Ile Asn Glu Ala Lys Asp Leu Leu Glu Gly 
            100                 105                 110         


Gln Ala Lys Lys 
        115     


<210> 6
<211> 498
<212> PRT
<213> Homo sapiens

<400> 6
Met Thr Val Phe Leu Ser Phe Ala Phe Leu Ala Ala Ile Leu Thr His 
1               5                   10                  15      


Ile Gly Cys Ser Asn Gln Arg Arg Ser Pro Glu Asn Ser Gly Arg Arg 
            20                  25                  30          


Tyr Asn Arg Ile Gln His Gly Gln Cys Ala Tyr Thr Phe Ile Leu Pro 
        35                  40                  45              


Glu His Asp Gly Asn Cys Arg Glu Ser Thr Thr Asp Gln Tyr Asn Thr 
    50                  55                  60                  


Asn Ala Leu Gln Arg Asp Ala Pro His Val Glu Pro Asp Phe Ser Ser 
65                  70                  75                  80  


Gln Lys Leu Gln His Leu Glu His Val Met Glu Asn Tyr Thr Gln Trp 
                85                  90                  95      


Leu Gln Lys Leu Glu Asn Tyr Ile Val Glu Asn Met Lys Ser Glu Met 
            100                 105                 110         


Ala Gln Ile Gln Gln Asn Ala Val Gln Asn His Thr Ala Thr Met Leu 
        115                 120                 125             


Glu Ile Gly Thr Ser Leu Leu Ser Gln Thr Ala Glu Gln Thr Arg Lys 
    130                 135                 140                 


Leu Thr Asp Val Glu Thr Gln Val Leu Asn Gln Thr Ser Arg Leu Glu 
145                 150                 155                 160 


Ile Gln Leu Leu Glu Asn Ser Leu Ser Thr Tyr Lys Leu Glu Lys Gln 
                165                 170                 175     


Leu Leu Gln Gln Thr Asn Glu Ile Leu Lys Ile His Glu Lys Asn Ser 
            180                 185                 190         


Leu Leu Glu His Lys Ile Leu Glu Met Glu Gly Lys His Lys Glu Glu 
        195                 200                 205             


Leu Asp Thr Leu Lys Glu Glu Lys Glu Asn Leu Gln Gly Leu Val Thr 
    210                 215                 220                 


Arg Gln Thr Tyr Ile Ile Gln Glu Leu Glu Lys Gln Leu Asn Arg Ala 
225                 230                 235                 240 


Thr Thr Asn Asn Ser Val Leu Gln Lys Gln Gln Leu Glu Leu Met Asp 
                245                 250                 255     


Thr Val His Asn Leu Val Asn Leu Cys Thr Lys Glu Gly Val Leu Leu 
            260                 265                 270         


Lys Gly Gly Lys Arg Glu Glu Glu Lys Pro Phe Arg Asp Cys Ala Asp 
        275                 280                 285             


Val Tyr Gln Ala Gly Phe Asn Lys Ser Gly Ile Tyr Thr Ile Tyr Ile 
    290                 295                 300                 


Asn Asn Met Pro Glu Pro Lys Lys Val Phe Cys Asn Met Asp Val Asn 
305                 310                 315                 320 


Gly Gly Gly Trp Thr Val Ile Gln His Arg Glu Asp Gly Ser Leu Asp 
                325                 330                 335     


Phe Gln Arg Gly Trp Lys Glu Tyr Lys Met Gly Phe Gly Asn Pro Ser 
            340                 345                 350         


Gly Glu Tyr Trp Leu Gly Asn Glu Phe Ile Phe Ala Ile Thr Ser Gln 
        355                 360                 365             


Arg Gln Tyr Met Leu Arg Ile Glu Leu Met Asp Trp Glu Gly Asn Arg 
    370                 375                 380                 


Ala Tyr Ser Gln Tyr Asp Arg Phe His Ile Gly Asn Glu Lys Gln Asn 
385                 390                 395                 400 


Tyr Arg Leu Tyr Leu Lys Gly His Thr Gly Thr Ala Gly Lys Gln Ser 
                405                 410                 415     


Ser Leu Ile Leu His Gly Ala Asp Phe Ser Thr Lys Asp Ala Asp Asn 
            420                 425                 430         


Asp Asn Cys Met Cys Lys Cys Ala Leu Met Leu Thr Gly Gly Trp Trp 
        435                 440                 445             


Phe Asp Ala Cys Gly Pro Ser Asn Leu Asn Gly Met Phe Tyr Thr Ala 
    450                 455                 460                 


Gly Gln Asn His Gly Lys Leu Asn Gly Ile Lys Trp His Tyr Phe Lys 
465                 470                 475                 480 


Gly Pro Ser Tyr Ser Leu Arg Ser Thr Thr Met Met Ile Arg Pro Leu 
                485                 490                 495     


Asp Phe 
        


<210> 7
<211> 249
<212> PRT
<213> Homo sapiens

<400> 7
Met Ala Ala Arg Arg Ser Gln Arg Arg Arg Gly Arg Arg Gly Glu Pro 
1               5                   10                  15      


Gly Thr Ala Leu Leu Val Pro Leu Ala Leu Gly Leu Gly Leu Ala Leu 
            20                  25                  30          


Ala Cys Leu Gly Leu Leu Leu Ala Val Val Ser Leu Gly Ser Arg Ala 
        35                  40                  45              


Ser Leu Ser Ala Gln Glu Pro Ala Gln Glu Glu Leu Val Ala Glu Glu 
    50                  55                  60                  


Asp Gln Asp Pro Ser Glu Leu Asn Pro Gln Thr Glu Glu Ser Gln Asp 
65                  70                  75                  80  


Pro Ala Pro Phe Leu Asn Arg Leu Val Arg Pro Arg Arg Ser Ala Pro 
                85                  90                  95      


Lys Gly Arg Lys Thr Arg Ala Arg Arg Ala Ile Ala Ala His Tyr Glu 
            100                 105                 110         


Val His Pro Arg Pro Gly Gln Asp Gly Ala Gln Ala Gly Val Asp Gly 
        115                 120                 125             


Thr Val Ser Gly Trp Glu Glu Ala Arg Ile Asn Ser Ser Ser Pro Leu 
    130                 135                 140                 


Arg Tyr Asn Arg Gln Ile Gly Glu Phe Ile Val Thr Arg Ala Gly Leu 
145                 150                 155                 160 


Tyr Tyr Leu Tyr Cys Gln Val His Phe Asp Glu Gly Lys Ala Val Tyr 
                165                 170                 175     


Leu Lys Leu Asp Leu Leu Val Asp Gly Val Leu Ala Leu Arg Cys Leu 
            180                 185                 190         


Glu Glu Phe Ser Ala Thr Ala Ala Ser Ser Leu Gly Pro Gln Leu Arg 
        195                 200                 205             


Leu Cys Gln Val Ser Gly Leu Leu Ala Leu Arg Pro Gly Ser Ser Leu 
    210                 215                 220                 


Arg Ile Arg Thr Leu Pro Trp Ala His Leu Lys Ala Ala Pro Phe Leu 
225                 230                 235                 240 


Thr Tyr Phe Gly Leu Phe Gln Val His 
                245                 


<210> 8
<211> 211
<212> PRT
<213> Homo sapiens

<400> 8
Met Ala Pro Leu Ala Glu Val Gly Gly Phe Leu Gly Gly Leu Glu Gly 
1               5                   10                  15      


Leu Gly Gln Gln Val Gly Ser His Phe Leu Leu Pro Pro Ala Gly Glu 
            20                  25                  30          


Arg Pro Pro Leu Leu Gly Glu Arg Arg Ser Ala Ala Glu Arg Ser Ala 
        35                  40                  45              


Arg Gly Gly Pro Gly Ala Ala Gln Leu Ala His Leu His Gly Ile Leu 
    50                  55                  60                  


Arg Arg Arg Gln Leu Tyr Cys Arg Thr Gly Phe His Leu Gln Ile Leu 
65                  70                  75                  80  


Pro Asp Gly Ser Val Gln Gly Thr Arg Gln Asp His Ser Leu Phe Gly 
                85                  90                  95      


Ile Leu Glu Phe Ile Ser Val Ala Val Gly Leu Val Ser Ile Arg Gly 
            100                 105                 110         


Val Asp Ser Gly Leu Tyr Leu Gly Met Asn Asp Lys Gly Glu Leu Tyr 
        115                 120                 125             


Gly Ser Glu Lys Leu Thr Ser Glu Cys Ile Phe Arg Glu Gln Phe Glu 
    130                 135                 140                 


Glu Asn Trp Tyr Asn Thr Tyr Ser Ser Asn Ile Tyr Lys His Gly Asp 
145                 150                 155                 160 


Thr Gly Arg Arg Tyr Phe Val Ala Leu Asn Lys Asp Gly Thr Pro Arg 
                165                 170                 175     


Asp Gly Ala Arg Ser Lys Arg His Gln Lys Phe Thr His Phe Leu Pro 
            180                 185                 190         


Arg Pro Val Asp Pro Glu Arg Val Pro Glu Leu Tyr Lys Asp Leu Leu 
        195                 200                 205             


Met Tyr Thr 
    210     


<210> 9
<211> 16
<212> PRT
<213> Homo sapiens

<400> 9
Gly Gly Pro Gly Ala Ala Gln Leu Ala His Leu His Gly Ile Leu Arg 
1               5                   10                  15      


<210> 10
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xHis tag

<400> 10
His His His His His His 
1               5       


<210>  11
<211>  424
<212>  PRT
<213>  Homo sapiens

<400>  11

Met Arg Ala Pro Gly Cys Gly Arg Leu Val Leu Pro Leu Leu Leu Leu 
1               5                   10                  15      


Ala Ala Ala Ala Leu Ala Glu Gly Asp Ala Lys Gly Leu Lys Glu Gly 
            20                  25                  30          


Glu Thr Pro Gly Asn Phe Met Glu Asp Glu Gln Trp Leu Ser Ser Ile 
        35                  40                  45              


Ser Gln Tyr Ser Gly Lys Ile Lys His Trp Asn Arg Phe Arg Asp Glu 
    50                  55                  60                  


Val Glu Asp Asp Tyr Ile Lys Ser Trp Glu Asp Asn Gln Gln Gly Asp 
65                  70                  75                  80  


Glu Ala Leu Asp Thr Thr Lys Asp Pro Cys Gln Lys Val Lys Cys Ser 
                85                  90                  95      


Arg His Lys Val Cys Ile Ala Gln Gly Tyr Gln Arg Ala Met Cys Ile 
            100                 105                 110         


Ser Arg Lys Lys Leu Glu His Arg Ile Lys Gln Pro Thr Val Lys Leu 
        115                 120                 125             


His Gly Asn Lys Asp Ser Ile Cys Lys Pro Cys His Met Ala Gln Leu 
    130                 135                 140                 


Ala Ser Val Cys Gly Ser Asp Gly His Thr Tyr Ser Ser Val Cys Lys 
145                 150                 155                 160 


Leu Glu Gln Gln Ala Cys Leu Ser Ser Lys Gln Leu Ala Val Arg Cys 
                165                 170                 175     


Glu Gly Pro Cys Pro Cys Pro Thr Glu Gln Ala Ala Thr Ser Thr Ala 
            180                 185                 190         


Asp Gly Lys Pro Glu Thr Cys Thr Gly Gln Asp Leu Ala Asp Leu Gly 
        195                 200                 205             


Asp Arg Leu Arg Asp Trp Phe Gln Leu Leu His Glu Asn Ser Lys Gln 
    210                 215                 220                 


Asn Gly Ser Ala Ser Ser Val Ala Gly Pro Ala Ser Gly Leu Asp Lys 
225                 230                 235                 240 


Ser Leu Gly Ala Ser Cys Lys Asp Ser Ile Gly Trp Met Phe Ser Lys 
                245                 250                 255     


Leu Asp Thr Ser Ala Asp Leu Phe Leu Asp Gln Thr Glu Leu Ala Ala 
            260                 265                 270         


Ile Asn Leu Asp Lys Tyr Glu Val Cys Ile Arg Pro Phe Phe Asn Ser 
        275                 280                 285             


Cys Asp Thr Tyr Lys Asp Gly Arg Val Ser Thr Ala Glu Trp Cys Phe 
    290                 295                 300                 


Cys Phe Trp Arg Glu Lys Pro Pro Cys Leu Ala Glu Leu Glu Arg Ile 
305                 310                 315                 320 


Gln Ile Gln Glu Ala Ala Lys Lys Lys Pro Gly Ile Phe Ile Pro Ser 
                325                 330                 335     


Cys Asp Glu Asp Gly Tyr Tyr Arg Lys Met Gln Cys Asp Gln Ser Ser 
            340                 345                 350         


Gly Asp Cys Trp Cys Val Asp Gln Leu Gly Leu Glu Leu Thr Gly Thr 
        355                 360                 365             


Arg Thr His Gly Ser Pro Asp Cys Asp Asp Ile Val Gly Phe Ser Gly 
    370                 375                 380                 


Asp Phe Gly Ser Gly Val Gly Trp Glu Asp Glu Glu Glu Lys Glu Thr 
385                 390                 395                 400 


Glu Glu Ala Gly Glu Glu Ala Glu Glu Glu Glu Gly Glu Ala Gly Glu 
                405                 410                 415     


Ala Asp Asp Gly Gly Tyr Ile Trp 
            420                 

