                         SEQUENCE LISTING

<110>  Cornell University
 
<120>  A MULTIPLEX ASSAY FOR THE DIAGNOSIS OF BRUCELLA CANIS INFECTION 
       IN DOGS

<130>  39359WO (9300-02-PC)

<150>  63/171,638
<151>  2021-04-07

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  250
<212>  PRT
<213>  Brucella canis

<400>  1

Met Asn Thr Arg Ala Ser Asn Phe Leu Ala Ala Ser Phe Ser Thr Ile 
1               5                   10                  15      


Met Leu Val Gly Ala Phe Ser Leu Pro Ala Phe Ala Gln Glu Asn Gln 
            20                  25                  30          


Met Thr Thr Gln Pro Ala Arg Ile Ala Val Thr Gly Glu Gly Met Met 
        35                  40                  45              


Thr Ala Ser Pro Asp Met Ala Ile Leu Asn Leu Ser Val Leu Arg Gln 
    50                  55                  60                  


Ala Lys Thr Ala Arg Glu Ala Met Thr Ala Asn Asn Glu Ala Met Thr 
65                  70                  75                  80  


Lys Val Leu Asp Ala Met Lys Lys Ala Gly Ile Glu Asp Arg Asp Leu 
                85                  90                  95      


Gln Thr Gly Gly Ile Asn Ile Gln Pro Ile Tyr Val Tyr Pro Asp Asp 
            100                 105                 110         


Lys Asn Asn Leu Lys Glu Pro Thr Ile Thr Gly Tyr Ser Val Ser Thr 
        115                 120                 125             


Ser Leu Thr Val Arg Val Arg Glu Leu Ala Asn Val Gly Lys Ile Leu 
    130                 135                 140                 


Asp Glu Ser Val Thr Leu Gly Val Asn Gln Gly Gly Asp Leu Asn Leu 
145                 150                 155                 160 


Val Asn Asp Asn Pro Ser Ala Val Ile Asn Glu Ala Arg Lys Arg Ala 
                165                 170                 175     


Val Ala Asn Ala Ile Ala Lys Ala Lys Thr Leu Ala Asp Ala Ala Gly 
            180                 185                 190         


Val Gly Leu Gly Arg Val Val Glu Ile Ser Glu Leu Ser Arg Pro Pro 
        195                 200                 205             


Met Pro Met Pro Ile Ala Arg Gly Gln Phe Arg Thr Met Leu Ala Ala 
    210                 215                 220                 


Ala Pro Asp Asn Ser Val Pro Ile Ala Ala Gly Glu Asn Ser Tyr Asn 
225                 230                 235                 240 


Val Ser Val Asn Val Val Phe Glu Ile Lys 
                245                 250 


<210>  2
<211>  240
<212>  PRT
<213>  Brucella canis

<400>  2

Met Lys Ser Val Ile Leu Ala Ser Ile Ala Ala Met Phe Ala Thr Ser 
1               5                   10                  15      


Ala Met Ala Ala Asp Val Val Val Ser Glu Pro Ser Ala Pro Thr Ala 
            20                  25                  30          


Ala Pro Val Asp Thr Phe Ser Trp Thr Gly Gly Tyr Ile Gly Ile Asn 
        35                  40                  45              


Ala Gly Tyr Ala Gly Gly Lys Phe Lys His Pro Phe Ser Ser Phe Asp 
    50                  55                  60                  


Lys Glu Asp Asn Glu Gln Val Ser Gly Ser Leu Asp Val Thr Ala Gly 
65                  70                  75                  80  


Gly Phe Val Gly Gly Val Gln Ala Gly Tyr Asn Trp Gln Leu Asp Asn 
                85                  90                  95      


Gly Val Val Leu Gly Ala Glu Thr Asp Phe Gln Gly Ser Ser Val Thr 
            100                 105                 110         


Gly Pro Ile Ser Ala Gly Ala Ser Gly Leu Glu Gly Lys Ala Glu Thr 
        115                 120                 125             


Lys Val Glu Trp Phe Gly Thr Val Arg Ala Arg Leu Gly Tyr Thr Ala 
    130                 135                 140                 


Thr Glu Arg Leu Met Val Tyr Gly Thr Gly Gly Leu Ala Tyr Gly Lys 
145                 150                 155                 160 


Val Lys Ser Ala Phe Asn Leu Gly Asp Asp Ala Ser Ala Leu His Thr 
                165                 170                 175     


Trp Ser Asp Lys Thr Lys Ala Gly Trp Thr Leu Gly Ala Gly Ala Glu 
            180                 185                 190         


Tyr Ala Ile Asn Asn Asn Trp Thr Leu Lys Ser Glu Tyr Leu Tyr Thr 
        195                 200                 205             


Asp Leu Gly Lys Arg Asn Leu Val Asp Val Asp Asn Ser Phe Leu Glu 
    210                 215                 220                 


Ser Lys Val Asn Phe His Thr Val Arg Val Gly Leu Asn Tyr Lys Phe 
225                 230                 235                 240 


<210>  3
<211>  33
<212>  DNA
<213>  Brucella canis

<400>  3
cgctcatgaa cactcgtgct agcaattttc tcg                                    33


<210>  4
<211>  42
<212>  DNA
<213>  Brucella canis

<400>  4
gcgggatccc ttgatttcaa aaacgacatt gaccgatacg tt                          42


<210>  5
<211>  29
<212>  DNA
<213>  Brucella canis

<400>  5
cgcccatggc acaggagaat cagatgacg                                         29


<210>  6
<211>  33
<212>  DNA
<213>  Brucella canis

<400>  6
cgcagatctc ttgatttcaa aaacgacatt gac                                    33


<210>  7
<211>  234
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His tagged BP26

<400>  7

Met Ala Gln Glu Asn Gln Met Thr Thr Gln Pro Ala Arg Ile Ala Val 
1               5                   10                  15      


Thr Gly Glu Gly Met Met Thr Ala Ser Pro Asp Met Ala Ile Leu Asn 
            20                  25                  30          


Leu Ser Val Leu Arg Gln Ala Lys Thr Ala Arg Glu Ala Met Thr Ala 
        35                  40                  45              


Asn Asn Glu Ala Met Thr Lys Val Leu Asp Ala Met Lys Lys Ala Gly 
    50                  55                  60                  


Ile Glu Asp Arg Asp Leu Gln Thr Gly Gly Ile Asn Ile Gln Pro Ile 
65                  70                  75                  80  


Tyr Val Tyr Pro Asp Asp Lys Asn Asn Leu Lys Glu Pro Thr Ile Thr 
                85                  90                  95      


Gly Tyr Ser Val Ser Thr Ser Leu Thr Val Arg Val Arg Glu Leu Ala 
            100                 105                 110         


Asn Val Gly Lys Ile Leu Asp Glu Ser Val Thr Leu Gly Val Asn Gln 
        115                 120                 125             


Gly Gly Asp Leu Asn Leu Val Asn Asp Asn Pro Ser Ala Val Ile Asn 
    130                 135                 140                 


Glu Ala Arg Lys Arg Ala Val Ala Asn Ala Ile Ala Lys Ala Lys Thr 
145                 150                 155                 160 


Leu Ala Asp Ala Ala Gly Val Gly Leu Gly Arg Val Val Glu Ile Ser 
                165                 170                 175     


Glu Leu Ser Arg Pro Pro Met Pro Met Pro Ile Ala Arg Gly Gln Phe 
            180                 185                 190         


Arg Thr Met Leu Ala Ala Ala Pro Asp Asn Ser Val Pro Ile Ala Ala 
        195                 200                 205             


Gly Glu Asn Ser Tyr Asn Val Ser Val Asn Val Val Phe Glu Ile Lys 
    210                 215                 220                 


Arg Ser Arg Ser His His His His His His 
225                 230                 


<210>  8
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Omp31pep (Fragment of Omp31 protein)

<400>  8

Asn Ala Gly Tyr Ala Gly Gly Lys Phe Lys His Pro Phe Ser Ser Phe 
1               5                   10                  15      


Asp Lys Glu Asp Asn Glu Gln Val Ser Gly Ser Leu Asp Val 
            20                  25                  30  


<210>  9
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PO1 (Fragment of Omp31pep)

<400>  9

Gly Lys Phe Lys His Pro Phe Ser Ser Phe Asp Lys Glu Asp Asn Glu 
1               5                   10                  15      


Gln 
    


<210>  10
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PO2 (Fragment of Omp31pep)

<400>  10

Asn Ala Gly Tyr Ala Gly Gly Lys Phe Lys His Pro Phe Ser Ser 
1               5                   10                  15  


<210>  11
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PO3 (Fragment of Omp31pep)

<400>  11

Phe Asp Lys Glu Asp Asn Glu Gln Val Ser Gly Ser Leu Asp Val 
1               5                   10                  15  


<210>  12
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PO4 (Fragment of Omp31pep)

<400>  12

Gly Gly Tyr Ile Gly Ile Asn Ala Gly Tyr Ala Gly Gly Lys Phe 
1               5                   10                  15  


<210>  13
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PO5 (Fragment of Omp31pep)

<400>  13

Glu Gln Val Ser Gly Ser Leu Asp Val Thr Ala Gly Gly Phe Val Gly 
1               5                   10                  15      


Gly Val Gln Ala 
            20  


<210>  14
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  UO2 (Fragment of Omp31 protein)

<400>  14

Met Ala Ala Asp Val Val Val Ser Glu Pro Ser Ala Pro Thr Ala Ala 
1               5                   10                  15      


Pro Val Asp Thr 
            20  


<210>  15
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  UO3 (Fragment of Omp31 protein)

<400>  15

Tyr Asn Trp Gln Leu Asp Asn Gly Val Val Leu Gly Ala Glu Thr Asp 
1               5                   10                  15      


Phe Gln Gly Ser Ser Val Thr Gly 
            20                  


<210>  16
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  UO4 (Fragment of Omp31 protein)

<400>  16

Met Val Tyr Gly Thr Gly Gly Leu Ala Tyr Gly Lys Val Lys Ser Ala 
1               5                   10                  15      


Phe Asn Leu Gly 
            20  


<210>  17
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  UO5 (Fragment of Omp31 protein)

<400>  17

Asn Ser Phe Leu Glu Ser Lys Val Asn Phe His Thr Val Arg Val Gly 
1               5                   10                  15      


Leu Asn Tyr Lys 
            20  


