                         SEQUENCE LISTING

<110>  Artiva Biotherapeutics, Inc. et al.
 
<120>  TREATMENT OF CANCER WITH NK CELLS AND AN EGFR TARGETED ANTIBODY

<130>  49755-0033WO1

<150>  US 63/290,361
<151>  2021-12-16

<150>  US 63/172,423
<151>  2021-04-08

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  254
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Glu Tyr Ala Ser Asp Ala Ser Leu Asp Pro Glu Ala Pro Trp Pro 
1               5                   10                  15      


Pro Ala Pro Arg Ala Arg Ala Cys Arg Val Leu Pro Trp Ala Leu Val 
            20                  25                  30          


Ala Gly Leu Leu Leu Leu Leu Leu Leu Ala Ala Ala Cys Ala Val Phe 
        35                  40                  45              


Leu Ala Cys Pro Trp Ala Val Ser Gly Ala Arg Ala Ser Pro Gly Ser 
    50                  55                  60                  


Ala Ala Ser Pro Arg Leu Arg Glu Gly Pro Glu Leu Ser Pro Asp Asp 
65                  70                  75                  80  


Pro Ala Gly Leu Leu Asp Leu Arg Gln Gly Met Phe Ala Gln Leu Val 
                85                  90                  95      


Ala Gln Asn Val Leu Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp 
            100                 105                 110         


Pro Gly Leu Ala Gly Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu 
        115                 120                 125             


Asp Thr Lys Glu Leu Val Val Ala Lys Ala Gly Val Tyr Tyr Val Phe 
    130                 135                 140                 


Phe Gln Leu Glu Leu Arg Arg Val Val Ala Gly Glu Gly Ser Gly Ser 
145                 150                 155                 160 


Val Ser Leu Ala Leu His Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala 
                165                 170                 175     


Ala Ala Leu Ala Leu Thr Val Asp Leu Pro Pro Ala Ser Ser Glu Ala 
            180                 185                 190         


Arg Asn Ser Ala Phe Gly Phe Gln Gly Arg Leu Leu His Leu Ser Ala 
        195                 200                 205             


Gly Gln Arg Leu Gly Val His Leu His Thr Glu Ala Arg Ala Arg His 
    210                 215                 220                 


Ala Trp Gln Leu Thr Gln Gly Ala Thr Val Leu Gly Leu Phe Arg Val 
225                 230                 235                 240 


Thr Pro Glu Ile Pro Ala Gly Leu Pro Ser Pro Arg Ser Glu 
                245                 250                 


<210>  2
<211>  223
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gln Asp Arg His Met Ile Arg Met Arg Gln Leu 
            20                  25                  30          


Ile Asp Ile Val Asp Gln Leu Lys Asn Tyr Val Asn Asp Leu Val Pro 
        35                  40                  45              


Glu Phe Leu Pro Ala Pro Glu Asp Val Glu Thr Asn Cys Glu Trp Ser 
    50                  55                  60                  


Ala Phe Ser Cys Phe Gln Lys Ala Gln Leu Lys Ser Ala Asn Thr Gly 
65                  70                  75                  80  


Asn Asn Glu Arg Ile Ile Asn Val Ser Ile Lys Lys Leu Lys Arg Lys 
                85                  90                  95      


Pro Pro Ser Thr Asn Ala Gly Arg Arg Gln Lys His Arg Leu Thr Cys 
            100                 105                 110         


Pro Ser Cys Asp Ser Tyr Glu Lys Lys Pro Pro Lys Glu Phe Leu Glu 
        115                 120                 125             


Arg Phe Lys Ser Leu Leu Gln Lys Met Ile His Gln His Leu Ser Ser 
    130                 135                 140                 


Arg Thr His Gly Ser Glu Asp Ser Ala Lys Pro Thr Thr Thr Pro Ala 
145                 150                 155                 160 


Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser 
                165                 170                 175     


Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr 
            180                 185                 190         


Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala 
        195                 200                 205             


Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr 
    210                 215                 220             


<210>  3
<211>  233
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Ser Thr Glu Ser Met Ile Arg Asp Val Glu Leu Ala Glu Glu Ala 
1               5                   10                  15      


Leu Pro Lys Lys Thr Gly Gly Pro Gln Gly Ser Arg Arg Cys Leu Phe 
            20                  25                  30          


Leu Ser Leu Phe Ser Phe Leu Ile Val Ala Gly Ala Thr Thr Leu Phe 
        35                  40                  45              


Cys Leu Leu His Phe Gly Val Ile Gly Pro Gln Arg Glu Glu Phe Pro 
    50                  55                  60                  


Arg Asp Leu Ser Leu Ile Ser Pro Leu Ala Gln Pro Val Arg Ser Ser 
65                  70                  75                  80  


Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His Val Val Ala Asn Pro 
                85                  90                  95      


Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn Arg Arg Ala Asn Ala Leu 
            100                 105                 110         


Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln Leu Val Val Pro Ser 
        115                 120                 125             


Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe Lys Gly Gln Gly 
    130                 135                 140                 


Cys Pro Ser Thr His Val Leu Leu Thr His Thr Ile Ser Arg Ile Ala 
145                 150                 155                 160 


Val Ser Tyr Gln Thr Lys Val Asn Leu Leu Ser Ala Ile Lys Ser Pro 
                165                 170                 175     


Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys Pro Trp Tyr Glu 
            180                 185                 190         


Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys Gly Asp Arg Leu 
        195                 200                 205             


Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe Ala Glu Ser Gly 
    210                 215                 220                 


Gln Val Tyr Phe Gly Ile Ile Ala Leu 
225                 230             


<210>  4
<211>  183
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Glu Arg Val Gln Pro Leu Glu Glu Asn Val Gly Asn Ala Ala Arg 
1               5                   10                  15      


Pro Arg Phe Glu Arg Asn Lys Leu Leu Leu Val Ala Ser Val Ile Gln 
            20                  25                  30          


Gly Leu Gly Leu Leu Leu Cys Phe Thr Tyr Ile Cys Leu His Phe Ser 
        35                  40                  45              


Ala Leu Gln Val Ser His Arg Tyr Pro Arg Ile Gln Ser Ile Lys Val 
    50                  55                  60                  


Gln Phe Thr Glu Tyr Lys Lys Glu Lys Gly Phe Ile Leu Thr Ser Gln 
65                  70                  75                  80  


Lys Glu Asp Glu Ile Met Lys Val Gln Asn Asn Ser Val Ile Ile Asn 
                85                  90                  95      


Cys Asp Gly Phe Tyr Leu Ile Ser Leu Lys Gly Tyr Phe Ser Gln Glu 
            100                 105                 110         


Val Asn Ile Ser Leu His Tyr Gln Lys Asp Glu Glu Pro Leu Phe Gln 
        115                 120                 125             


Leu Lys Lys Val Arg Ser Val Asn Ser Leu Met Val Ala Ser Leu Thr 
    130                 135                 140                 


Tyr Lys Asp Lys Val Tyr Leu Asn Val Thr Thr Asp Asn Thr Ser Leu 
145                 150                 155                 160 


Asp Asp Phe His Val Asn Gly Gly Glu Leu Ile Leu Ile His Gln Asn 
                165                 170                 175     


Pro Gly Glu Phe Cys Val Leu 
            180             


<210>  5
<211>  41
<212>  PRT
<213>  Homo sapiens

<400>  5

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  6
<211>  123
<212>  DNA
<213>  Homo sapiens

<400>  6
aggagtaaga ggagcaggct cctgcacagt gactacatga acatgactcc ccgccgcccc       60

gggcccaccc gcaagcatta ccagccctat gccccaccac gcgacttcgc agcctatcgc      120

tcc                                                                    123


<210>  7
<211>  123
<212>  DNA
<213>  Artificial

<220>
<223>  Codon optimized CD28 intracellular signaling domain

<400>  7
cggagcaaga ggtcccgcct gctgcacagc gactatatga acatgacccc acggagaccc       60

ggccctacac ggaaacatta ccagccctat gctccacccc gggacttcgc agcttacaga      120

agt                                                                    123


<210>  8
<211>  22
<212>  PRT
<213>  Homo sapiens

<400>  8

Glu Arg Val Gln Pro Leu Glu Glu Asn Val Gly Asn Ala Ala Arg Pro 
1               5                   10                  15      


Arg Phe Glu Arg Asn Lys 
            20          


<210>  9
<211>  66
<212>  DNA
<213>  Homo sapiens

<400>  9
gaaagggtcc aacccctgga agagaatgtg ggaaatgcag ccaggccaag attcgagagg       60

aacaag                                                                  66


<210>  10
<211>  66
<212>  DNA
<213>  Artificial

<220>
<223>  Codon optimized OX40L intracellular signaling domain

<400>  10
gaaagagtgc agcccctgga agagaatgtc gggaatgccg ctcgcccaag atttgaaagg       60

aacaaa                                                                  66


<210>  11
<211>  112
<212>  PRT
<213>  Homo sapiens

<400>  11

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  12
<211>  336
<212>  DNA
<213>  Homo sapiens

<400>  12
agagtgaagt tcagcaggag cgcagacgcc cccgcgtacc agcagggcca gaaccagctc       60

tataacgagc tcaatctagg acgaagagag gagtacgatg ttttggacaa gagacgtggc      120

cgggaccctg agatgggggg aaagccgaga aggaagaacc ctcaggaagg cctgtacaat      180

gaactgcaga aagataagat ggcggaggcc tacagtgaga ttgggatgaa aggcgagcgc      240

cggaggggca aggggcacga tggcctttac cagggtctca gtacagccac caaggacacc      300

tacgacgccc ttcacatgca ggccctgccc cctcgc                                336


<210>  13
<211>  336
<212>  DNA
<213>  Artificial

<220>
<223>  Codon optimized CD3zeta signaling domain

<400>  13
cgagtgaagt tcagcaggtc cgccgacgct cctgcatacc agcagggaca gaaccagctg       60

tataacgagc tgaatctggg ccggagagag gaatacgacg tgctggacaa aaggcggggc      120

cgggaccccg aaatgggagg gaagccacga cggaaaaacc cccaggaggg cctgtacaat      180

gagctgcaaa aggacaaaat ggccgaggct tattctgaaa tcgggatgaa gggagagaga      240

aggcgcggaa aaggccacga tggcctgtac caggggctga gcaccgctac aaaggacacc      300

tatgatgcac tgcacatgca ggccctgccc cctcgg                                336


<210>  14
<211>  21
<212>  PRT
<213>  Artificial

<220>
<223>  T2A cleavage site

<400>  14

Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu 
1               5                   10                  15      


Glu Asn Pro Gly Pro 
            20      


<210>  15
<211>  63
<212>  DNA
<213>  Artificial

<220>
<223>  T2A cleavage site

<400>  15
ggctcaggtg aggggcgcgg gagcctgctg acttgtgggg atgtagagga aaatcctggt       60

cct                                                                     63


<210>  16
<211>  162
<212>  PRT
<213>  Homo sapiens

<400>  16

Met Arg Ile Ser Lys Pro His Leu Arg Ser Ile Ser Ile Gln Cys Tyr 
1               5                   10                  15      


Leu Cys Leu Leu Leu Asn Ser His Phe Leu Thr Glu Ala Gly Ile His 
            20                  25                  30          


Val Phe Ile Leu Gly Cys Phe Ser Ala Gly Leu Pro Lys Thr Glu Ala 
        35                  40                  45              


Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys Ile Glu Asp Leu Ile 
    50                  55                  60                  


Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr Glu Ser Asp Val His 
65                  70                  75                  80  


Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe Leu Leu Glu Leu Gln 
                85                  90                  95      


Val Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile His Asp Thr Val Glu 
            100                 105                 110         


Asn Leu Ile Ile Leu Ala Asn Asn Ser Leu Ser Ser Asn Gly Asn Val 
        115                 120                 125             


Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu Glu Glu Lys Asn Ile 
    130                 135                 140                 


Lys Glu Phe Leu Gln Ser Phe Val His Ile Val Gln Met Phe Ile Asn 
145                 150                 155                 160 


Thr Ser 
        


<210>  17
<211>  489
<212>  DNA
<213>  Homo sapiens

<400>  17
atgagaatca gcaaaccaca cctccggagc atatcaatcc agtgttactt gtgccttctt       60

ttgaactccc atttcctcac cgaggcaggc attcatgtgt tcatattggg gtgctttagt      120

gctgggcttc cgaaaacgga agctaactgg gtaaacgtca tcagtgacct taaaaaaatt      180

gaggatctta tccaatcaat gcacatcgac gcgactctct acacagaatc tgacgtacac      240

ccgtcatgca aagtcacggc aatgaagtgt tttcttctcg agctccaagt aatttccctg      300

gagtctggcg atgcctccat ccacgatacg gttgaaaatc tgattatatt ggccaacaat      360

agcctcagtt ctaacggtaa cgtgactgaa agtggctgca aagagtgcga agagctcgaa      420

gaaaagaata tcaaggagtt cctccaatca tttgttcaca ttgtgcaaat gtttatcaac      480

acctcttga                                                              489


<210>  18
<211>  489
<212>  DNA
<213>  Homo sapiens

<400>  18
atgcgcataa gtaagcctca tctgcggtcc atttctatac aatgttatct gtgcttgctt       60

ttgaactccc actttcttac ggaagcaggc attcatgtgt tcattctggg ttgtttttct      120

gccgggctgc ccaaaaccga ggccaactgg gtcaacgtga tcagcgacct caagaagatc      180

gaggatttga ttcaaagtat gcatatagac gccacactct atactgagtc cgacgttcac      240

ccgagttgta aagttacggc tatgaagtgc tttttgttgg aactccaggt gatttccctt      300

gaatccggcg atgcgagcat ccacgatacg gtagagaatc ttattattct ggcgaataat      360

tctctgtctt caaatgggaa tgtaactgag agcggttgta aagaatgcga agaacttgaa      420

gaaaagaata tcaaggaatt tcttcagagt ttcgtgcata ttgttcaaat gttcatcaac      480

acatcctga                                                              489


<210>  19
<211>  175
<212>  PRT
<213>  Artificial

<220>
<223>  CD28/OX40L/CD3zeta

<400>  19

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser Glu Arg Val Gln Pro Leu Glu 
        35                  40                  45              


Glu Asn Val Gly Asn Ala Ala Arg Pro Arg Phe Glu Arg Asn Lys Arg 
    50                  55                  60                  


Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln 
65                  70                  75                  80  


Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
                85                  90                  95      


Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
            100                 105                 110         


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
        115                 120                 125             


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
    130                 135                 140                 


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
145                 150                 155                 160 


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                165                 170                 175 


<210>  20
<211>  358
<212>  PRT
<213>  Artificial

<220>
<223>  CD28/OX40L/CD3zeta/T2A/IL1-5

<400>  20

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser Glu Arg Val Gln Pro Leu Glu 
        35                  40                  45              


Glu Asn Val Gly Asn Ala Ala Arg Pro Arg Phe Glu Arg Asn Lys Arg 
    50                  55                  60                  


Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln 
65                  70                  75                  80  


Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
                85                  90                  95      


Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
            100                 105                 110         


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
        115                 120                 125             


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
    130                 135                 140                 


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
145                 150                 155                 160 


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Gly 
                165                 170                 175     


Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu 
            180                 185                 190         


Asn Pro Gly Pro Met Arg Ile Ser Lys Pro His Leu Arg Ser Ile Ser 
        195                 200                 205             


Ile Gln Cys Tyr Leu Cys Leu Leu Leu Asn Ser His Phe Leu Thr Glu 
    210                 215                 220                 


Ala Gly Ile His Val Phe Ile Leu Gly Cys Phe Ser Ala Gly Leu Pro 
225                 230                 235                 240 


Lys Thr Glu Ala Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys Ile 
                245                 250                 255     


Glu Asp Leu Ile Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr Glu 
            260                 265                 270         


Ser Asp Val His Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe Leu 
        275                 280                 285             


Leu Glu Leu Gln Val Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile His 
    290                 295                 300                 


Asp Thr Val Glu Asn Leu Ile Ile Leu Ala Asn Asn Ser Leu Ser Ser 
305                 310                 315                 320 


Asn Gly Asn Val Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu Glu 
                325                 330                 335     


Glu Lys Asn Ile Lys Glu Phe Leu Gln Ser Phe Val His Ile Val Gln 
            340                 345                 350         


Met Phe Ile Asn Thr Ser 
        355             


<210>  21
<211>  609
<212>  PRT
<213>  Homo sapiens

<400>  21

Met Lys Trp Val Thr Phe Ile Ser Leu Leu Phe Leu Phe Ser Ser Ala 
1               5                   10                  15      


Tyr Ser Arg Gly Val Phe Arg Arg Asp Ala His Lys Ser Glu Val Ala 
            20                  25                  30          


His Arg Phe Lys Asp Leu Gly Glu Glu Asn Phe Lys Ala Leu Val Leu 
        35                  40                  45              


Ile Ala Phe Ala Gln Tyr Leu Gln Gln Cys Pro Phe Glu Asp His Val 
    50                  55                  60                  


Lys Leu Val Asn Glu Val Thr Glu Phe Ala Lys Thr Cys Val Ala Asp 
65                  70                  75                  80  


Glu Ser Ala Glu Asn Cys Asp Lys Ser Leu His Thr Leu Phe Gly Asp 
                85                  90                  95      


Lys Leu Cys Thr Val Ala Thr Leu Arg Glu Thr Tyr Gly Glu Met Ala 
            100                 105                 110         


Asp Cys Cys Ala Lys Gln Glu Pro Glu Arg Asn Glu Cys Phe Leu Gln 
        115                 120                 125             


His Lys Asp Asp Asn Pro Asn Leu Pro Arg Leu Val Arg Pro Glu Val 
    130                 135                 140                 


Asp Val Met Cys Thr Ala Phe His Asp Asn Glu Glu Thr Phe Leu Lys 
145                 150                 155                 160 


Lys Tyr Leu Tyr Glu Ile Ala Arg Arg His Pro Tyr Phe Tyr Ala Pro 
                165                 170                 175     


Glu Leu Leu Phe Phe Ala Lys Arg Tyr Lys Ala Ala Phe Thr Glu Cys 
            180                 185                 190         


Cys Gln Ala Ala Asp Lys Ala Ala Cys Leu Leu Pro Lys Leu Asp Glu 
        195                 200                 205             


Leu Arg Asp Glu Gly Lys Ala Ser Ser Ala Lys Gln Arg Leu Lys Cys 
    210                 215                 220                 


Ala Ser Leu Gln Lys Phe Gly Glu Arg Ala Phe Lys Ala Trp Ala Val 
225                 230                 235                 240 


Ala Arg Leu Ser Gln Arg Phe Pro Lys Ala Glu Phe Ala Glu Val Ser 
                245                 250                 255     


Lys Leu Val Thr Asp Leu Thr Lys Val His Thr Glu Cys Cys His Gly 
            260                 265                 270         


Asp Leu Leu Glu Cys Ala Asp Asp Arg Ala Asp Leu Ala Lys Tyr Ile 
        275                 280                 285             


Cys Glu Asn Gln Asp Ser Ile Ser Ser Lys Leu Lys Glu Cys Cys Glu 
    290                 295                 300                 


Lys Pro Leu Leu Glu Lys Ser His Cys Ile Ala Glu Val Glu Asn Asp 
305                 310                 315                 320 


Glu Met Pro Ala Asp Leu Pro Ser Leu Ala Ala Asp Phe Val Glu Ser 
                325                 330                 335     


Lys Asp Val Cys Lys Asn Tyr Ala Glu Ala Lys Asp Val Phe Leu Gly 
            340                 345                 350         


Met Phe Leu Tyr Glu Tyr Ala Arg Arg His Pro Asp Tyr Ser Val Val 
        355                 360                 365             


Leu Leu Leu Arg Leu Ala Lys Thr Tyr Glu Thr Thr Leu Glu Lys Cys 
    370                 375                 380                 


Cys Ala Ala Ala Asp Pro His Glu Cys Tyr Ala Lys Val Phe Asp Glu 
385                 390                 395                 400 


Phe Lys Pro Leu Val Glu Glu Pro Gln Asn Leu Ile Lys Gln Asn Cys 
                405                 410                 415     


Glu Leu Phe Glu Gln Leu Gly Glu Tyr Lys Phe Gln Asn Ala Leu Leu 
            420                 425                 430         


Val Arg Tyr Thr Lys Lys Val Pro Gln Val Ser Thr Pro Thr Leu Val 
        435                 440                 445             


Glu Val Ser Arg Asn Leu Gly Lys Val Gly Ser Lys Cys Cys Lys His 
    450                 455                 460                 


Pro Glu Ala Lys Arg Met Pro Cys Ala Glu Asp Tyr Leu Ser Val Val 
465                 470                 475                 480 


Leu Asn Gln Leu Cys Val Leu His Glu Lys Thr Pro Val Ser Asp Arg 
                485                 490                 495     


Val Thr Lys Cys Cys Thr Glu Ser Leu Val Asn Arg Arg Pro Cys Phe 
            500                 505                 510         


Ser Ala Leu Glu Val Asp Glu Thr Tyr Val Pro Lys Glu Phe Asn Ala 
        515                 520                 525             


Glu Thr Phe Thr Phe His Ala Asp Ile Cys Thr Leu Ser Glu Lys Glu 
    530                 535                 540                 


Arg Gln Ile Lys Lys Gln Thr Ala Leu Val Glu Leu Val Lys His Lys 
545                 550                 555                 560 


Pro Lys Ala Thr Lys Glu Gln Leu Lys Ala Val Met Asp Asp Phe Ala 
                565                 570                 575     


Ala Phe Val Glu Lys Cys Cys Lys Ala Asp Asp Lys Glu Thr Cys Phe 
            580                 585                 590         


Ala Glu Glu Gly Lys Lys Leu Val Ala Ala Ser Gln Ala Ala Leu Gly 
        595                 600                 605             


Leu 
    


<210>  22
<211>  603
<212>  DNA
<213>  Artificial

<220>
<223>  Puromycin Resistance Gene

<400>  22
atggccaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc       60

gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg      120

gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc      180

gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg      240

gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc      300

ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag      360

gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg      420

ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc      480

ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc      540

gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc      600

tga                                                                    603


