                         SEQUENCE LISTING

<110>  Artiva Biotherapeutics, Inc.
       GC Cell Corporation
 
<120>  CHIMERIC ANTIGEN RECEPTOR COMPRISING AN ANTI-CD19 ANTIBODY OR 
       ANTIGEN-BINDING FRAGMENT THEREOF AND NATURAL KILLER CELLS 
       COMPRISING THE SAME

<130>  49755-0019WO1

<140>  PCT/US2022/023654
<141>  2022-04-06

<150>  US 63/172,416
<151>  2021-04-08

<160>  74    

<170>  PatentIn version 3.5

<210>  1
<211>  254
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Glu Tyr Ala Ser Asp Ala Ser Leu Asp Pro Glu Ala Pro Trp Pro 
1               5                   10                  15      


Pro Ala Pro Arg Ala Arg Ala Cys Arg Val Leu Pro Trp Ala Leu Val 
            20                  25                  30          


Ala Gly Leu Leu Leu Leu Leu Leu Leu Ala Ala Ala Cys Ala Val Phe 
        35                  40                  45              


Leu Ala Cys Pro Trp Ala Val Ser Gly Ala Arg Ala Ser Pro Gly Ser 
    50                  55                  60                  


Ala Ala Ser Pro Arg Leu Arg Glu Gly Pro Glu Leu Ser Pro Asp Asp 
65                  70                  75                  80  


Pro Ala Gly Leu Leu Asp Leu Arg Gln Gly Met Phe Ala Gln Leu Val 
                85                  90                  95      


Ala Gln Asn Val Leu Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp 
            100                 105                 110         


Pro Gly Leu Ala Gly Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu 
        115                 120                 125             


Asp Thr Lys Glu Leu Val Val Ala Lys Ala Gly Val Tyr Tyr Val Phe 
    130                 135                 140                 


Phe Gln Leu Glu Leu Arg Arg Val Val Ala Gly Glu Gly Ser Gly Ser 
145                 150                 155                 160 


Val Ser Leu Ala Leu His Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala 
                165                 170                 175     


Ala Ala Leu Ala Leu Thr Val Asp Leu Pro Pro Ala Ser Ser Glu Ala 
            180                 185                 190         


Arg Asn Ser Ala Phe Gly Phe Gln Gly Arg Leu Leu His Leu Ser Ala 
        195                 200                 205             


Gly Gln Arg Leu Gly Val His Leu His Thr Glu Ala Arg Ala Arg His 
    210                 215                 220                 


Ala Trp Gln Leu Thr Gln Gly Ala Thr Val Leu Gly Leu Phe Arg Val 
225                 230                 235                 240 


Thr Pro Glu Ile Pro Ala Gly Leu Pro Ser Pro Arg Ser Glu 
                245                 250                 


<210>  2
<211>  223
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gln Asp Arg His Met Ile Arg Met Arg Gln Leu 
            20                  25                  30          


Ile Asp Ile Val Asp Gln Leu Lys Asn Tyr Val Asn Asp Leu Val Pro 
        35                  40                  45              


Glu Phe Leu Pro Ala Pro Glu Asp Val Glu Thr Asn Cys Glu Trp Ser 
    50                  55                  60                  


Ala Phe Ser Cys Phe Gln Lys Ala Gln Leu Lys Ser Ala Asn Thr Gly 
65                  70                  75                  80  


Asn Asn Glu Arg Ile Ile Asn Val Ser Ile Lys Lys Leu Lys Arg Lys 
                85                  90                  95      


Pro Pro Ser Thr Asn Ala Gly Arg Arg Gln Lys His Arg Leu Thr Cys 
            100                 105                 110         


Pro Ser Cys Asp Ser Tyr Glu Lys Lys Pro Pro Lys Glu Phe Leu Glu 
        115                 120                 125             


Arg Phe Lys Ser Leu Leu Gln Lys Met Ile His Gln His Leu Ser Ser 
    130                 135                 140                 


Arg Thr His Gly Ser Glu Asp Ser Ala Lys Pro Thr Thr Thr Pro Ala 
145                 150                 155                 160 


Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser 
                165                 170                 175     


Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr 
            180                 185                 190         


Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala 
        195                 200                 205             


Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr 
    210                 215                 220             


<210>  3
<211>  233
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Ser Thr Glu Ser Met Ile Arg Asp Val Glu Leu Ala Glu Glu Ala 
1               5                   10                  15      


Leu Pro Lys Lys Thr Gly Gly Pro Gln Gly Ser Arg Arg Cys Leu Phe 
            20                  25                  30          


Leu Ser Leu Phe Ser Phe Leu Ile Val Ala Gly Ala Thr Thr Leu Phe 
        35                  40                  45              


Cys Leu Leu His Phe Gly Val Ile Gly Pro Gln Arg Glu Glu Phe Pro 
    50                  55                  60                  


Arg Asp Leu Ser Leu Ile Ser Pro Leu Ala Gln Pro Val Arg Ser Ser 
65                  70                  75                  80  


Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His Val Val Ala Asn Pro 
                85                  90                  95      


Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn Arg Arg Ala Asn Ala Leu 
            100                 105                 110         


Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln Leu Val Val Pro Ser 
        115                 120                 125             


Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe Lys Gly Gln Gly 
    130                 135                 140                 


Cys Pro Ser Thr His Val Leu Leu Thr His Thr Ile Ser Arg Ile Ala 
145                 150                 155                 160 


Val Ser Tyr Gln Thr Lys Val Asn Leu Leu Ser Ala Ile Lys Ser Pro 
                165                 170                 175     


Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys Pro Trp Tyr Glu 
            180                 185                 190         


Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys Gly Asp Arg Leu 
        195                 200                 205             


Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe Ala Glu Ser Gly 
    210                 215                 220                 


Gln Val Tyr Phe Gly Ile Ile Ala Leu 
225                 230             


<210>  4
<211>  183
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Glu Arg Val Gln Pro Leu Glu Glu Asn Val Gly Asn Ala Ala Arg 
1               5                   10                  15      


Pro Arg Phe Glu Arg Asn Lys Leu Leu Leu Val Ala Ser Val Ile Gln 
            20                  25                  30          


Gly Leu Gly Leu Leu Leu Cys Phe Thr Tyr Ile Cys Leu His Phe Ser 
        35                  40                  45              


Ala Leu Gln Val Ser His Arg Tyr Pro Arg Ile Gln Ser Ile Lys Val 
    50                  55                  60                  


Gln Phe Thr Glu Tyr Lys Lys Glu Lys Gly Phe Ile Leu Thr Ser Gln 
65                  70                  75                  80  


Lys Glu Asp Glu Ile Met Lys Val Gln Asn Asn Ser Val Ile Ile Asn 
                85                  90                  95      


Cys Asp Gly Phe Tyr Leu Ile Ser Leu Lys Gly Tyr Phe Ser Gln Glu 
            100                 105                 110         


Val Asn Ile Ser Leu His Tyr Gln Lys Asp Glu Glu Pro Leu Phe Gln 
        115                 120                 125             


Leu Lys Lys Val Arg Ser Val Asn Ser Leu Met Val Ala Ser Leu Thr 
    130                 135                 140                 


Tyr Lys Asp Lys Val Tyr Leu Asn Val Thr Thr Asp Asn Thr Ser Leu 
145                 150                 155                 160 


Asp Asp Phe His Val Asn Gly Gly Glu Leu Ile Leu Ile His Gln Asn 
                165                 170                 175     


Pro Gly Glu Phe Cys Val Leu 
            180             


<210>  5
<211>  41
<212>  PRT
<213>  Homo sapiens

<400>  5

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  6
<211>  123
<212>  DNA
<213>  Homo sapiens

<400>  6
aggagtaaga ggagcaggct cctgcacagt gactacatga acatgactcc ccgccgcccc       60

gggcccaccc gcaagcatta ccagccctat gccccaccac gcgacttcgc agcctatcgc      120

tcc                                                                    123


<210>  7
<211>  123
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon Optimized CD28 intracellular signaling domain

<400>  7
cggagcaaga ggtcccgcct gctgcacagc gactatatga acatgacccc acggagaccc       60

ggccctacac ggaaacatta ccagccctat gctccacccc gggacttcgc agcttacaga      120

agt                                                                    123


<210>  8
<211>  22
<212>  PRT
<213>  Homo sapiens

<400>  8

Glu Arg Val Gln Pro Leu Glu Glu Asn Val Gly Asn Ala Ala Arg Pro 
1               5                   10                  15      


Arg Phe Glu Arg Asn Lys 
            20          


<210>  9
<211>  17
<212>  PRT
<213>  Homo sapiens

<400>  9

Leu Glu Glu Asn Val Gly Asn Ala Ala Arg Pro Arg Phe Glu Arg Asn 
1               5                   10                  15      


Lys 
    


<210>  10
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  10

Arg Pro Arg Phe Glu Arg Asn Lys 
1               5               


<210>  11
<211>  66
<212>  DNA
<213>  Homo sapiens

<400>  11
gaaagggtcc aacccctgga agagaatgtg ggaaatgcag ccaggccaag attcgagagg       60

aacaag                                                                  66


<210>  12
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized OX40L intracellular signaling domain

<400>  12
gaaagagtgc agcccctgga agagaatgtc gggaatgccg ctcgcccaag atttgaaagg       60

aacaaa                                                                  66


<210>  13
<211>  112
<212>  PRT
<213>  Homo sapiens

<400>  13

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  14
<211>  336
<212>  DNA
<213>  Homo sapiens

<400>  14
agagtgaagt tcagcaggag cgcagacgcc cccgcgtacc agcagggcca gaaccagctc       60

tataacgagc tcaatctagg acgaagagag gagtacgatg ttttggacaa gagacgtggc      120

cgggaccctg agatgggggg aaagccgaga aggaagaacc ctcaggaagg cctgtacaat      180

gaactgcaga aagataagat ggcggaggcc tacagtgaga ttgggatgaa aggcgagcgc      240

cggaggggca aggggcacga tggcctttac cagggtctca gtacagccac caaggacacc      300

tacgacgccc ttcacatgca ggccctgccc cctcgc                                336


<210>  15
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized CD3zeta signaling domain

<400>  15
cgagtgaagt tcagcaggtc cgccgacgct cctgcatacc agcagggaca gaaccagctg       60

tataacgagc tgaatctggg ccggagagag gaatacgacg tgctggacaa aaggcggggc      120

cgggaccccg aaatgggagg gaagccacga cggaaaaacc cccaggaggg cctgtacaat      180

gagctgcaaa aggacaaaat ggccgaggct tattctgaaa tcgggatgaa gggagagaga      240

aggcgcggaa aaggccacga tggcctgtac caggggctga gcaccgctac aaaggacacc      300

tatgatgcac tgcacatgca ggccctgccc cctcgg                                336


<210>  16
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2A cleavage motif


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa can be any naturally occurring amino acid

<400>  16

Gly Asp Val Glu Xaa Asn Pro Gly Pro 
1               5                   


<210>  17
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  T2A cleavage site

<400>  17

Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu 
1               5                   10                  15      


Glu Asn Pro Gly Pro 
            20      


<210>  18
<211>  63
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  T2A cleavage site

<400>  18

Gly Gly Cys Thr Cys Ala Gly Gly Thr Gly Ala Gly Gly Gly Gly Cys 
1               5                   10                  15      


Gly Cys Gly Gly Gly Ala Gly Cys Cys Thr Gly Cys Thr Gly Ala Cys 
            20                  25                  30          


Thr Thr Gly Thr Gly Gly Gly Gly Ala Thr Gly Thr Ala Gly Ala Gly 
        35                  40                  45              


Gly Ala Ala Ala Ala Thr Cys Cys Thr Gly Gly Thr Cys Cys Thr 
    50                  55                  60              


<210>  19
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P2A cleavage site

<400>  19

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  20
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E2A cleavage site

<400>  20

Gly Ser Gly Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp 
1               5                   10                  15      


Val Glu Ser Asn Pro Gly Pro 
            20              


<210>  21
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  F2A cleavage site

<400>  21

Gly Ser Gly Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala 
1               5                   10                  15      


Gly Asp Val Glu Ser Asn Pro Gly Pro 
            20                  25  


<210>  22
<211>  162
<212>  PRT
<213>  Homo sapiens

<400>  22

Met Arg Ile Ser Lys Pro His Leu Arg Ser Ile Ser Ile Gln Cys Tyr 
1               5                   10                  15      


Leu Cys Leu Leu Leu Asn Ser His Phe Leu Thr Glu Ala Gly Ile His 
            20                  25                  30          


Val Phe Ile Leu Gly Cys Phe Ser Ala Gly Leu Pro Lys Thr Glu Ala 
        35                  40                  45              


Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys Ile Glu Asp Leu Ile 
    50                  55                  60                  


Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr Glu Ser Asp Val His 
65                  70                  75                  80  


Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe Leu Leu Glu Leu Gln 
                85                  90                  95      


Val Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile His Asp Thr Val Glu 
            100                 105                 110         


Asn Leu Ile Ile Leu Ala Asn Asn Ser Leu Ser Ser Asn Gly Asn Val 
        115                 120                 125             


Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu Glu Glu Lys Asn Ile 
    130                 135                 140                 


Lys Glu Phe Leu Gln Ser Phe Val His Ile Val Gln Met Phe Ile Asn 
145                 150                 155                 160 


Thr Ser 
        


<210>  23
<211>  489
<212>  DNA
<213>  Homo sapiens

<400>  23
atgagaatca gcaaaccaca cctccggagc atatcaatcc agtgttactt gtgccttctt       60

ttgaactccc atttcctcac cgaggcaggc attcatgtgt tcatattggg gtgctttagt      120

gctgggcttc cgaaaacgga agctaactgg gtaaacgtca tcagtgacct taaaaaaatt      180

gaggatctta tccaatcaat gcacatcgac gcgactctct acacagaatc tgacgtacac      240

ccgtcatgca aagtcacggc aatgaagtgt tttcttctcg agctccaagt aatttccctg      300

gagtctggcg atgcctccat ccacgatacg gttgaaaatc tgattatatt ggccaacaat      360

agcctcagtt ctaacggtaa cgtgactgaa agtggctgca aagagtgcga agagctcgaa      420

gaaaagaata tcaaggagtt cctccaatca tttgttcaca ttgtgcaaat gtttatcaac      480

acctcttga                                                              489


<210>  24
<211>  489
<212>  DNA
<213>  Homo sapiens

<400>  24
atgcgcataa gtaagcctca tctgcggtcc atttctatac aatgttatct gtgcttgctt       60

ttgaactccc actttcttac ggaagcaggc attcatgtgt tcattctggg ttgtttttct      120

gccgggctgc ccaaaaccga ggccaactgg gtcaacgtga tcagcgacct caagaagatc      180

gaggatttga ttcaaagtat gcatatagac gccacactct atactgagtc cgacgttcac      240

ccgagttgta aagttacggc tatgaagtgc tttttgttgg aactccaggt gatttccctt      300

gaatccggcg atgcgagcat ccacgatacg gtagagaatc ttattattct ggcgaataat      360

tctctgtctt caaatgggaa tgtaactgag agcggttgta aagaatgcga agaacttgaa      420

gaaaagaata tcaaggaatt tcttcagagt ttcgtgcata ttgttcaaat gttcatcaac      480

acatcctga                                                              489


<210>  25
<211>  175
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28/OX40L/CD3zeta

<400>  25

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser Glu Arg Val Gln Pro Leu Glu 
        35                  40                  45              


Glu Asn Val Gly Asn Ala Ala Arg Pro Arg Phe Glu Arg Asn Lys Arg 
    50                  55                  60                  


Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln 
65                  70                  75                  80  


Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
                85                  90                  95      


Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
            100                 105                 110         


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
        115                 120                 125             


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
    130                 135                 140                 


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
145                 150                 155                 160 


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                165                 170                 175 


<210>  26
<211>  358
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28/OX40L/CD3zeta/T2A/IL1-5

<400>  26

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser Glu Arg Val Gln Pro Leu Glu 
        35                  40                  45              


Glu Asn Val Gly Asn Ala Ala Arg Pro Arg Phe Glu Arg Asn Lys Arg 
    50                  55                  60                  


Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln 
65                  70                  75                  80  


Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
                85                  90                  95      


Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
            100                 105                 110         


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
        115                 120                 125             


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
    130                 135                 140                 


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
145                 150                 155                 160 


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Gly 
                165                 170                 175     


Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu 
            180                 185                 190         


Asn Pro Gly Pro Met Arg Ile Ser Lys Pro His Leu Arg Ser Ile Ser 
        195                 200                 205             


Ile Gln Cys Tyr Leu Cys Leu Leu Leu Asn Ser His Phe Leu Thr Glu 
    210                 215                 220                 


Ala Gly Ile His Val Phe Ile Leu Gly Cys Phe Ser Ala Gly Leu Pro 
225                 230                 235                 240 


Lys Thr Glu Ala Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys Ile 
                245                 250                 255     


Glu Asp Leu Ile Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr Glu 
            260                 265                 270         


Ser Asp Val His Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe Leu 
        275                 280                 285             


Leu Glu Leu Gln Val Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile His 
    290                 295                 300                 


Asp Thr Val Glu Asn Leu Ile Ile Leu Ala Asn Asn Ser Leu Ser Ser 
305                 310                 315                 320 


Asn Gly Asn Val Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu Glu 
                325                 330                 335     


Glu Lys Asn Ile Lys Glu Phe Leu Gln Ser Phe Val His Ile Val Gln 
            340                 345                 350         


Met Phe Ile Asn Thr Ser 
        355             


<210>  27
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  27

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  28
<211>  63
<212>  DNA
<213>  Homo sapiens

<400>  28
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccg                                                                     63


<210>  29
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon Optimized CD8alpha signal sequence

<400>  29
atggcacttc ctgttacagc cctcctgctc ccactggctt tgctgctgca tgctgcacga       60

ccg                                                                     63


<210>  30
<211>  242
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD19 scFv

<400>  30

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Gly Gly Gly Ser 
            100                 105                 110         


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Gln Glu 
        115                 120                 125             


Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys 
    130                 135                 140                 


Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg 
145                 150                 155                 160 


Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser 
                165                 170                 175     


Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile 
            180                 185                 190         


Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln 
        195                 200                 205             


Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly 
    210                 215                 220                 


Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val 
225                 230                 235                 240 


Ser Ser 
        


<210>  31
<211>  726
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  anti-CD19 scFv

<400>  31
gacattcaaa tgacacaaac tacgagctcc ctctccgcct ccctcggtga cagggtgacc       60

atctcatgtc gggctagtca ggacataagc aaatacttga actggtacca gcaaaagcca      120

gacgggaccg tgaagctgct catctatcac actagccgcc ttcactctgg ggtcccctct      180

cgctttagcg gttccggctc tggtacagac tattccctta ctatttcaaa tcttgagcag      240

gaagatatcg ctacatattt ttgtcaacag gggaatactc ttccgtatac attcgggggc      300

ggaacaaaac ttgaaattac aggcggcggt ggttcaggag gtggtgggag cggtgggggg      360

ggatcagaag ttaagctgca agaatcaggg cccggactgg tagcgcccag tcaaagtctt      420

tctgttactt gtacagtatc cggggtgtcc cttccagatt acggggtatc ttggattagg      480

caacctcctc ggaaaggatt ggagtggttg ggtgttatct ggggcagtga aaccacttac      540

tataatagtg ctcttaaaag tagactcacc ataataaagg ataatagcaa gagccaggta      600

ttcctgaaaa tgaacagctt gcaaacggat gataccgcta tctactactg cgccaaacac      660

tattattatg gcggtagtta cgctatggat tattggggcc aaggaacgtc cgtcaccgtt      720

agtagc                                                                 726


<210>  32
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG1 hinge

<400>  32

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro 
1               5                   10          


<210>  33
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IgG1 hinge

<400>  33
gagccaaagt cttgcgacaa gactcatacc tgcccg                                 36


<210>  34
<211>  219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Spacer (IgG1, CH2 (mu) - CH3

<400>  34

Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro 
1               5                   10                  15      


Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr 
            20                  25                  30          


Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn 
        35                  40                  45              


Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg 
    50                  55                  60                  


Glu Glu Gln Tyr Gln Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val 
65                  70                  75                  80  


Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser 
                85                  90                  95      


Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys 
            100                 105                 110         


Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp 
        115                 120                 125             


Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe 
    130                 135                 140                 


Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 
145                 150                 155                 160 


Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 
                165                 170                 175     


Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly 
            180                 185                 190         


Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr 
        195                 200                 205             


Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    210                 215                 


<210>  35
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Spacer (IgG1, CH2 (mu) - CH3

<400>  35
ccttgtcccg caccacctgt ggcaggtccc agcgtgtttc tgtttccgcc caagcccaag       60

gacacactca tgatctctag gacacccgag gttacatgcg tcgtggttga tgtttctcat      120

gaagaccccg aagtgaagtt taactggtat gtcgacggag ttgaggtcca taatgctaaa      180

acaaaaccac gcgaggaaca atatcaaagc acctatcggg tcgttagtgt ccttactgta      240

cttcaccagg actggctcaa cggaaaggaa tataagtgca aagtcagcaa taaagccttg      300

ccagcgccca tcgaaaaaac tatcagcaag gccaag                                336


<210>  36
<211>  220
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Spacer (IgG1, CH2 (mu) - CH3

<400>  36

Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe 
1               5                   10                  15      


Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val 
            20                  25                  30          


Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe 
        35                  40                  45              


Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro 
    50                  55                  60                  


Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr 
65                  70                  75                  80  


Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val 
                85                  90                  95      


Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala 
            100                 105                 110         


Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg 
        115                 120                 125             


Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly 
    130                 135                 140                 


Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro 
145                 150                 155                 160 


Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser 
                165                 170                 175     


Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln 
            180                 185                 190         


Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His 
        195                 200                 205             


Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    210                 215                 220 


<210>  37
<211>  657
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Spacer (IgG1, CH2 (mu) - CH3

<400>  37
ccttgtcccg caccacctgt ggcaggtccc agcgtgtttc tgtttccgcc caagcccaag       60

gacacactca tgatctctag gacacccgag gttacatgcg tcgtggttga tgtttctcat      120

gaagaccccg aagtgaagtt taactggtat gtcgacggag ttgaggtcca taatgctaaa      180

acaaaaccac gcgaggaaca atatcaaagc acctatcggg tcgttagtgt ccttactgta      240

cttcaccagg actggctcaa cggaaaggaa tataagtgca aagtcagcaa taaagccttg      300

ccagcgccca tcgaaaaaac tatcagcaag gccaagggcc aacccagaga accccaagtg      360

tacacactcc ctcctagccg ggatgaattg accaaaaatc aagtctctct cacttgtctg      420

gtaaagggat tctatccaag tgatattgct gtcgaatggg agagcaacgg gcaaccggag      480

aacaattaca aaacgacacc tcccgtcctt gatagtgacg ggtccttctt cctgtattca      540

aagcttacag tcgacaaaag tcgctggcag caggggaatg tctttagctg tagtgtcatg      600

cacgaagctt tgcataacca ctacacgcaa aagtctctta gcctgtcccc aggaaag         657


<210>  38
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 transmembrane domain

<400>  38

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25          


<210>  39
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD28 transmembrane domain

<400>  39
ttctgggtac tggtggttgt gggcggcgtt ctggcttgct attcactcct cgttacggtt       60

gcatttataa tattttgggt a                                                 81


<210>  40
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 transmembrane domain

<400>  40

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr Leu Tyr Cys 
            20                  


<210>  41
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD8 transmembrane domain

<400>  41
atctacattt gggcccctct ggctggaaca tgtggcgtgc tgctgctgtc cctggtcatt       60

actctgtatt gt                                                           72


<210>  42
<211>  697
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD19 CAR-WT-28 (with signal)

<400>  42

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 
    130                 135                 140                 


Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser 
145                 150                 155                 160 


Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly 
                165                 170                 175     


Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly 
            180                 185                 190         


Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser 
        195                 200                 205             


Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys 
    210                 215                 220                 


Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys 
225                 230                 235                 240 


His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly 
                245                 250                 255     


Thr Ser Val Thr Val Ser Ser Glu Pro Lys Ser Cys Asp Lys Thr His 
            260                 265                 270         


Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val 
        275                 280                 285             


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
    290                 295                 300                 


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
305                 310                 315                 320 


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
                325                 330                 335     


Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
            340                 345                 350         


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
        355                 360                 365             


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
    370                 375                 380                 


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
385                 390                 395                 400 


Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
                405                 410                 415     


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
            420                 425                 430         


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
        435                 440                 445             


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
    450                 455                 460                 


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
465                 470                 475                 480 


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Phe 
                485                 490                 495     


Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu 
            500                 505                 510         


Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg 
        515                 520                 525             


Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro 
    530                 535                 540                 


Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala 
545                 550                 555                 560 


Tyr Arg Ser Glu Arg Val Gln Pro Leu Glu Glu Asn Val Gly Asn Ala 
                565                 570                 575     


Ala Arg Pro Arg Phe Glu Arg Asn Lys Arg Val Lys Phe Ser Arg Ser 
            580                 585                 590         


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
        595                 600                 605             


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
    610                 615                 620                 


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
625                 630                 635                 640 


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
                645                 650                 655     


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
            660                 665                 670         


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
        675                 680                 685             


Leu His Met Gln Ala Leu Pro Pro Arg 
    690                 695         


<210>  43
<211>  2091
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD19 CAR-WT-28

<400>  43
atggcacttc ctgttacagc cctcctgctc ccactggctt tgctgctgca tgctgcacga       60

ccggacattc aaatgacaca aactacgagc tccctctccg cctccctcgg tgacagggtg      120

accatctcat gtcgggctag tcaggacata agcaaatact tgaactggta ccagcaaaag      180

ccagacggga ccgtgaagct gctcatctat cacactagcc gccttcactc tggggtcccc      240

tctcgcttta gcggttccgg ctctggtaca gactattccc ttactatttc aaatcttgag      300

caggaagata tcgctacata tttttgtcaa caggggaata ctcttccgta tacattcggg      360

ggcggaacaa aacttgaaat tacaggcggc ggtggttcag gaggtggtgg gagcggtggg      420

gggggatcag aagttaagct gcaagaatca gggcccggac tggtagcgcc cagtcaaagt      480

ctttctgtta cttgtacagt atccggggtg tcccttccag attacggggt atcttggatt      540

aggcaacctc ctcggaaagg attggagtgg ttgggtgtta tctggggcag tgaaaccact      600

tactataata gtgctcttaa aagtagactc accataataa aggataatag caagagccag      660

gtattcctga aaatgaacag cttgcaaacg gatgataccg ctatctacta ctgcgccaaa      720

cactattatt atggcggtag ttacgctatg gattattggg gccaaggaac gtccgtcacc      780

gttagtagcg agccaaagtc ttgcgacaag actcatacct gcccgccttg tcccgcacca      840

gagttgctgg gtggtcccag cgtgtttctg tttccgccca agcccaagga cacactcatg      900

atctctagga cacccgaggt tacatgcgtc gtggttgatg tttctcatga agaccccgaa      960

gtgaagttta actggtatgt cgacggagtt gaggtccata atgctaaaac aaaaccacgc     1020

gaggaacaat ataatagcac ctatcgggtc gttagtgtcc ttactgtact tcaccaggac     1080

tggctcaacg gaaaggaata taagtgcaaa gtcagcaata aagccttgcc agcgcccatc     1140

gaaaaaacta tcagcaaggc caagggccaa cccagagaac cccaagtgta cacactccct     1200

cctagccggg atgaattgac caaaaatcaa gtctctctca cttgtctggt aaagggattc     1260

tatccaagtg atattgctgt cgaatgggag agcaacgggc aaccggagaa caattacaaa     1320

acgacacctc ccgtccttga tagtgacggg tccttcttcc tgtattcaaa gcttacagtc     1380

gacaaaagtc gctggcagca ggggaatgtc tttagctgta gtgtcatgca cgaagctttg     1440

cataaccact acacgcaaaa gtctcttagc ctgtccccag gaaagttctg ggtactggtg     1500

gttgtgggcg gcgttctggc ttgctattca ctcctcgtta cggttgcatt tataatattt     1560

tgggtacgct caaagcgctc aagattgctc cattctgatt acatgaacat gacccctcgc     1620

cggccgggtc ccactcgcaa acactaccaa ccttatgccc ctccgcggga tttcgcggcc     1680

tacaggtcag agagagtaca acctcttgag gagaatgtcg gcaacgctgc caggccgcgg     1740

tttgagcgaa ataagcgagt caagttttct cgaagtgccg acgctcctgc ctatcagcaa     1800

ggccagaatc aactgtataa cgagcttaac ttgggccgga gggaggagta cgatgtgctg     1860

gataagcgca ggggacggga cccggaaatg ggcggaaagc ccaggcggaa aaacccacag     1920

gaaggactct acaatgaact ccaaaaggac aaaatggcag aggcctattc agagattggg     1980

atgaagggag aaaggaggcg aggaaaaggg cacgacggct tgtatcaggg cttgtctacg     2040

gccactaaag acacctatga tgccctgcat atgcaggcac ttccccccag a              2091


<210>  44
<211>  880
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD19 CAR-WT-28 with T2A and IL-15

<400>  44

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 
    130                 135                 140                 


Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser 
145                 150                 155                 160 


Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly 
                165                 170                 175     


Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly 
            180                 185                 190         


Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser 
        195                 200                 205             


Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys 
    210                 215                 220                 


Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys 
225                 230                 235                 240 


His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly 
                245                 250                 255     


Thr Ser Val Thr Val Ser Ser Glu Pro Lys Ser Cys Asp Lys Thr His 
            260                 265                 270         


Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val 
        275                 280                 285             


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
    290                 295                 300                 


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
305                 310                 315                 320 


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
                325                 330                 335     


Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
            340                 345                 350         


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
        355                 360                 365             


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
    370                 375                 380                 


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
385                 390                 395                 400 


Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
                405                 410                 415     


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
            420                 425                 430         


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
        435                 440                 445             


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
    450                 455                 460                 


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
465                 470                 475                 480 


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Phe 
                485                 490                 495     


Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu 
            500                 505                 510         


Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg 
        515                 520                 525             


Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro 
    530                 535                 540                 


Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala 
545                 550                 555                 560 


Tyr Arg Ser Glu Arg Val Gln Pro Leu Glu Glu Asn Val Gly Asn Ala 
                565                 570                 575     


Ala Arg Pro Arg Phe Glu Arg Asn Lys Arg Val Lys Phe Ser Arg Ser 
            580                 585                 590         


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
        595                 600                 605             


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
    610                 615                 620                 


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
625                 630                 635                 640 


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
                645                 650                 655     


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
            660                 665                 670         


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
        675                 680                 685             


Leu His Met Gln Ala Leu Pro Pro Arg Gly Ser Gly Glu Gly Arg Gly 
    690                 695                 700                 


Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Arg 
705                 710                 715                 720 


Ile Ser Lys Pro His Leu Arg Ser Ile Ser Ile Gln Cys Tyr Leu Cys 
                725                 730                 735     


Leu Leu Leu Asn Ser His Phe Leu Thr Glu Ala Gly Ile His Val Phe 
            740                 745                 750         


Ile Leu Gly Cys Phe Ser Ala Gly Leu Pro Lys Thr Glu Ala Asn Trp 
        755                 760                 765             


Val Asn Val Ile Ser Asp Leu Lys Lys Ile Glu Asp Leu Ile Gln Ser 
    770                 775                 780                 


Met His Ile Asp Ala Thr Leu Tyr Thr Glu Ser Asp Val His Pro Ser 
785                 790                 795                 800 


Cys Lys Val Thr Ala Met Lys Cys Phe Leu Leu Glu Leu Gln Val Ile 
                805                 810                 815     


Ser Leu Glu Ser Gly Asp Ala Ser Ile His Asp Thr Val Glu Asn Leu 
            820                 825                 830         


Ile Ile Leu Ala Asn Asn Ser Leu Ser Ser Asn Gly Asn Val Thr Glu 
        835                 840                 845             


Ser Gly Cys Lys Glu Cys Glu Glu Leu Glu Glu Lys Asn Ile Lys Glu 
    850                 855                 860                 


Phe Leu Gln Ser Phe Val His Ile Val Gln Met Phe Ile Asn Thr Ser 
865                 870                 875                 880 


<210>  45
<211>  2643
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD19 CAR-WT-28 with T2A and IL-15

<400>  45
atggcacttc ctgttacagc cctcctgctc ccactggctt tgctgctgca tgctgcacga       60

ccggacattc aaatgacaca aactacgagc tccctctccg cctccctcgg tgacagggtg      120

accatctcat gtcgggctag tcaggacata agcaaatact tgaactggta ccagcaaaag      180

ccagacggga ccgtgaagct gctcatctat cacactagcc gccttcactc tggggtcccc      240

tctcgcttta gcggttccgg ctctggtaca gactattccc ttactatttc aaatcttgag      300

caggaagata tcgctacata tttttgtcaa caggggaata ctcttccgta tacattcggg      360

ggcggaacaa aacttgaaat tacaggcggc ggtggttcag gaggtggtgg gagcggtggg      420

gggggatcag aagttaagct gcaagaatca gggcccggac tggtagcgcc cagtcaaagt      480

ctttctgtta cttgtacagt atccggggtg tcccttccag attacggggt atcttggatt      540

aggcaacctc ctcggaaagg attggagtgg ttgggtgtta tctggggcag tgaaaccact      600

tactataata gtgctcttaa aagtagactc accataataa aggataatag caagagccag      660

gtattcctga aaatgaacag cttgcaaacg gatgataccg ctatctacta ctgcgccaaa      720

cactattatt atggcggtag ttacgctatg gattattggg gccaaggaac gtccgtcacc      780

gttagtagcg agccaaagtc ttgcgacaag actcatacct gcccgccttg tcccgcacca      840

gagttgctgg gtggtcccag cgtgtttctg tttccgccca agcccaagga cacactcatg      900

atctctagga cacccgaggt tacatgcgtc gtggttgatg tttctcatga agaccccgaa      960

gtgaagttta actggtatgt cgacggagtt gaggtccata atgctaaaac aaaaccacgc     1020

gaggaacaat ataatagcac ctatcgggtc gttagtgtcc ttactgtact tcaccaggac     1080

tggctcaacg gaaaggaata taagtgcaaa gtcagcaata aagccttgcc agcgcccatc     1140

gaaaaaacta tcagcaaggc caagggccaa cccagagaac cccaagtgta cacactccct     1200

cctagccggg atgaattgac caaaaatcaa gtctctctca cttgtctggt aaagggattc     1260

tatccaagtg atattgctgt cgaatgggag agcaacgggc aaccggagaa caattacaaa     1320

acgacacctc ccgtccttga tagtgacggg tccttcttcc tgtattcaaa gcttacagtc     1380

gacaaaagtc gctggcagca ggggaatgtc tttagctgta gtgtcatgca cgaagctttg     1440

cataaccact acacgcaaaa gtctcttagc ctgtccccag gaaagttctg ggtactggtg     1500

gttgtgggcg gcgttctggc ttgctattca ctcctcgtta cggttgcatt tataatattt     1560

tgggtacgct caaagcgctc aagattgctc cattctgatt acatgaacat gacccctcgc     1620

cggccgggtc ccactcgcaa acactaccaa ccttatgccc ctccgcggga tttcgcggcc     1680

tacaggtcag agagagtaca acctcttgag gagaatgtcg gcaacgctgc caggccgcgg     1740

tttgagcgaa ataagcgagt caagttttct cgaagtgccg acgctcctgc ctatcagcaa     1800

ggccagaatc aactgtataa cgagcttaac ttgggccgga gggaggagta cgatgtgctg     1860

gataagcgca ggggacggga cccggaaatg ggcggaaagc ccaggcggaa aaacccacag     1920

gaaggactct acaatgaact ccaaaaggac aaaatggcag aggcctattc agagattggg     1980

atgaagggag aaaggaggcg aggaaaaggg cacgacggct tgtatcaggg cttgtctacg     2040

gccactaaag acacctatga tgccctgcat atgcaggcac ttccccccag aggaagtggg     2100

gaaggccgag gttcattgct cacctgtggc gatgtggaag aaaatccagg tccgatgcgc     2160

ataagtaagc ctcatctgcg gtccatttct atacaatgtt atctgtgctt gcttttgaac     2220

tcccactttc ttacggaagc aggcattcat gtgttcattc tgggttgttt ttctgccggg     2280

ctgcccaaaa ccgaggccaa ctgggtcaac gtgatcagcg acctcaagaa gatcgaggat     2340

ttgattcaaa gtatgcatat agacgccaca ctctatactg agtccgacgt tcacccgagt     2400

tgtaaagtta cggctatgaa gtgctttttg ttggaactcc aggtgatttc ccttgaatcc     2460

ggcgatgcga gcatccacga tacggtagag aatcttatta ttctggcgaa taattctctg     2520

tcttcaaatg ggaatgtaac tgagagcggt tgtaaagaat gcgaagaact tgaagaaaag     2580

aatatcaagg aatttcttca gagtttcgtg catattgttc aaatgttcat caacacatcc     2640

tga                                                                   2643


<210>  46
<211>  696
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD19 CAR-MU-28 (with signal)

<400>  46

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 
    130                 135                 140                 


Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser 
145                 150                 155                 160 


Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly 
                165                 170                 175     


Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly 
            180                 185                 190         


Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser 
        195                 200                 205             


Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys 
    210                 215                 220                 


Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys 
225                 230                 235                 240 


His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly 
                245                 250                 255     


Thr Ser Val Thr Val Ser Ser Glu Pro Lys Ser Cys Asp Lys Thr His 
            260                 265                 270         


Thr Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser Val Phe 
        275                 280                 285             


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
    290                 295                 300                 


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
305                 310                 315                 320 


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
                325                 330                 335     


Lys Pro Arg Glu Glu Gln Tyr Gln Ser Thr Tyr Arg Val Val Ser Val 
            340                 345                 350         


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
        355                 360                 365             


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
    370                 375                 380                 


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
385                 390                 395                 400 


Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
                405                 410                 415     


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
            420                 425                 430         


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
        435                 440                 445             


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
    450                 455                 460                 


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
465                 470                 475                 480 


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Phe Trp 
                485                 490                 495     


Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val 
            500                 505                 510         


Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu 
        515                 520                 525             


Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr 
    530                 535                 540                 


Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr 
545                 550                 555                 560 


Arg Ser Glu Arg Val Gln Pro Leu Glu Glu Asn Val Gly Asn Ala Ala 
                565                 570                 575     


Arg Pro Arg Phe Glu Arg Asn Lys Arg Val Lys Phe Ser Arg Ser Ala 
            580                 585                 590         


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
        595                 600                 605             


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
    610                 615                 620                 


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
625                 630                 635                 640 


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
                645                 650                 655     


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
            660                 665                 670         


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
        675                 680                 685             


His Met Gln Ala Leu Pro Pro Arg 
    690                 695     


<210>  47
<211>  2088
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD19 CAR-MU-28

<400>  47
atggcacttc ctgttacagc cctcctgctc ccactggctt tgctgctgca tgctgcacga       60

ccggacattc aaatgacaca aactacgagc tccctctccg cctccctcgg tgacagggtg      120

accatctcat gtcgggctag tcaggacata agcaaatact tgaactggta ccagcaaaag      180

ccagacggga ccgtgaagct gctcatctat cacactagcc gccttcactc tggggtcccc      240

tctcgcttta gcggttccgg ctctggtaca gactattccc ttactatttc aaatcttgag      300

caggaagata tcgctacata tttttgtcaa caggggaata ctcttccgta tacattcggg      360

ggcggaacaa aacttgaaat tacaggcggc ggtggttcag gaggtggtgg gagcggtggg      420

gggggatcag aagttaagct gcaagaatca gggcccggac tggtagcgcc cagtcaaagt      480

ctttctgtta cttgtacagt atccggggtg tcccttccag attacggggt atcttggatt      540

aggcaacctc ctcggaaagg attggagtgg ttgggtgtta tctggggcag tgaaaccact      600

tactataata gtgctcttaa aagtagactc accataataa aggataatag caagagccag      660

gtattcctga aaatgaacag cttgcaaacg gatgataccg ctatctacta ctgcgccaaa      720

cactattatt atggcggtag ttacgctatg gattattggg gccaaggaac gtccgtcacc      780

gttagtagcg agccaaagtc ttgcgacaag actcatacct gcccgccttg tcccgcacca      840

cctgtggcag gtcccagcgt gtttctgttt ccgcccaagc ccaaggacac actcatgatc      900

tctaggacac ccgaggttac atgcgtcgtg gttgatgttt ctcatgaaga ccccgaagtg      960

aagtttaact ggtatgtcga cggagttgag gtccataatg ctaaaacaaa accacgcgag     1020

gaacaatatc aaagcaccta tcgggtcgtt agtgtcctta ctgtacttca ccaggactgg     1080

ctcaacggaa aggaatataa gtgcaaagtc agcaataaag ccttgccagc gcccatcgaa     1140

aaaactatca gcaaggccaa gggccaaccc agagaacccc aagtgtacac actccctcct     1200

agccgggatg aattgaccaa aaatcaagtc tctctcactt gtctggtaaa gggattctat     1260

ccaagtgata ttgctgtcga atgggagagc aacgggcaac cggagaacaa ttacaaaacg     1320

acacctcccg tccttgatag tgacgggtcc ttcttcctgt attcaaagct tacagtcgac     1380

aaaagtcgct ggcagcaggg gaatgtcttt agctgtagtg tcatgcacga agctttgcat     1440

aaccactaca cgcaaaagtc tcttagcctg tccccaggaa agttctgggt actggtggtt     1500

gtgggcggcg ttctggcttg ctattcactc ctcgttacgg ttgcatttat aatattttgg     1560

gtacgctcaa agcgctcaag attgctccat tctgattaca tgaacatgac ccctcgccgg     1620

ccgggtccca ctcgcaaaca ctaccaacct tatgcccctc cgcgggattt cgcggcctac     1680

aggtcagaga gagtacaacc tcttgaggag aatgtcggca acgctgccag gccgcggttt     1740

gagcgaaata agcgagtcaa gttttctcga agtgccgacg ctcctgccta tcagcaaggc     1800

cagaatcaac tgtataacga gcttaacttg ggccggaggg aggagtacga tgtgctggat     1860

aagcgcaggg gacgggaccc ggaaatgggc ggaaagccca ggcggaaaaa cccacaggaa     1920

ggactctaca atgaactcca aaaggacaaa atggcagagg cctattcaga gattgggatg     1980

aagggagaaa ggaggcgagg aaaagggcac gacggcttgt atcagggctt gtctacggcc     2040

actaaagaca cctatgatgc cctgcatatg caggcacttc cccccaga                  2088


<210>  48
<211>  879
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD19 CAR-MU-28 with T2A and IL-15

<400>  48

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 
    130                 135                 140                 


Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser 
145                 150                 155                 160 


Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly 
                165                 170                 175     


Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly 
            180                 185                 190         


Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser 
        195                 200                 205             


Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys 
    210                 215                 220                 


Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys 
225                 230                 235                 240 


His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly 
                245                 250                 255     


Thr Ser Val Thr Val Ser Ser Glu Pro Lys Ser Cys Asp Lys Thr His 
            260                 265                 270         


Thr Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser Val Phe 
        275                 280                 285             


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
    290                 295                 300                 


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
305                 310                 315                 320 


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
                325                 330                 335     


Lys Pro Arg Glu Glu Gln Tyr Gln Ser Thr Tyr Arg Val Val Ser Val 
            340                 345                 350         


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
        355                 360                 365             


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
    370                 375                 380                 


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
385                 390                 395                 400 


Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
                405                 410                 415     


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
            420                 425                 430         


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
        435                 440                 445             


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
    450                 455                 460                 


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
465                 470                 475                 480 


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Phe Trp 
                485                 490                 495     


Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val 
            500                 505                 510         


Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu 
        515                 520                 525             


Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr 
    530                 535                 540                 


Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr 
545                 550                 555                 560 


Arg Ser Glu Arg Val Gln Pro Leu Glu Glu Asn Val Gly Asn Ala Ala 
                565                 570                 575     


Arg Pro Arg Phe Glu Arg Asn Lys Arg Val Lys Phe Ser Arg Ser Ala 
            580                 585                 590         


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
        595                 600                 605             


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
    610                 615                 620                 


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
625                 630                 635                 640 


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
                645                 650                 655     


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
            660                 665                 670         


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
        675                 680                 685             


His Met Gln Ala Leu Pro Pro Arg Gly Ser Gly Glu Gly Arg Gly Ser 
    690                 695                 700                 


Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Arg Ile 
705                 710                 715                 720 


Ser Lys Pro His Leu Arg Ser Ile Ser Ile Gln Cys Tyr Leu Cys Leu 
                725                 730                 735     


Leu Leu Asn Ser His Phe Leu Thr Glu Ala Gly Ile His Val Phe Ile 
            740                 745                 750         


Leu Gly Cys Phe Ser Ala Gly Leu Pro Lys Thr Glu Ala Asn Trp Val 
        755                 760                 765             


Asn Val Ile Ser Asp Leu Lys Lys Ile Glu Asp Leu Ile Gln Ser Met 
    770                 775                 780                 


His Ile Asp Ala Thr Leu Tyr Thr Glu Ser Asp Val His Pro Ser Cys 
785                 790                 795                 800 


Lys Val Thr Ala Met Lys Cys Phe Leu Leu Glu Leu Gln Val Ile Ser 
                805                 810                 815     


Leu Glu Ser Gly Asp Ala Ser Ile His Asp Thr Val Glu Asn Leu Ile 
            820                 825                 830         


Ile Leu Ala Asn Asn Ser Leu Ser Ser Asn Gly Asn Val Thr Glu Ser 
        835                 840                 845             


Gly Cys Lys Glu Cys Glu Glu Leu Glu Glu Lys Asn Ile Lys Glu Phe 
    850                 855                 860                 


Leu Gln Ser Phe Val His Ile Val Gln Met Phe Ile Asn Thr Ser 
865                 870                 875                 


<210>  49
<211>  2640
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD19 CAR-MU-28 with T2A and IL-15

<400>  49
atggcacttc ctgttacagc cctcctgctc ccactggctt tgctgctgca tgctgcacga       60

ccggacattc aaatgacaca aactacgagc tccctctccg cctccctcgg tgacagggtg      120

accatctcat gtcgggctag tcaggacata agcaaatact tgaactggta ccagcaaaag      180

ccagacggga ccgtgaagct gctcatctat cacactagcc gccttcactc tggggtcccc      240

tctcgcttta gcggttccgg ctctggtaca gactattccc ttactatttc aaatcttgag      300

caggaagata tcgctacata tttttgtcaa caggggaata ctcttccgta tacattcggg      360

ggcggaacaa aacttgaaat tacaggcggc ggtggttcag gaggtggtgg gagcggtggg      420

gggggatcag aagttaagct gcaagaatca gggcccggac tggtagcgcc cagtcaaagt      480

ctttctgtta cttgtacagt atccggggtg tcccttccag attacggggt atcttggatt      540

aggcaacctc ctcggaaagg attggagtgg ttgggtgtta tctggggcag tgaaaccact      600

tactataata gtgctcttaa aagtagactc accataataa aggataatag caagagccag      660

gtattcctga aaatgaacag cttgcaaacg gatgataccg ctatctacta ctgcgccaaa      720

cactattatt atggcggtag ttacgctatg gattattggg gccaaggaac gtccgtcacc      780

gttagtagcg agccaaagtc ttgcgacaag actcatacct gcccgccttg tcccgcacca      840

cctgtggcag gtcccagcgt gtttctgttt ccgcccaagc ccaaggacac actcatgatc      900

tctaggacac ccgaggttac atgcgtcgtg gttgatgttt ctcatgaaga ccccgaagtg      960

aagtttaact ggtatgtcga cggagttgag gtccataatg ctaaaacaaa accacgcgag     1020

gaacaatatc aaagcaccta tcgggtcgtt agtgtcctta ctgtacttca ccaggactgg     1080

ctcaacggaa aggaatataa gtgcaaagtc agcaataaag ccttgccagc gcccatcgaa     1140

aaaactatca gcaaggccaa gggccaaccc agagaacccc aagtgtacac actccctcct     1200

agccgggatg aattgaccaa aaatcaagtc tctctcactt gtctggtaaa gggattctat     1260

ccaagtgata ttgctgtcga atgggagagc aacgggcaac cggagaacaa ttacaaaacg     1320

acacctcccg tccttgatag tgacgggtcc ttcttcctgt attcaaagct tacagtcgac     1380

aaaagtcgct ggcagcaggg gaatgtcttt agctgtagtg tcatgcacga agctttgcat     1440

aaccactaca cgcaaaagtc tcttagcctg tccccaggaa agttctgggt actggtggtt     1500

gtgggcggcg ttctggcttg ctattcactc ctcgttacgg ttgcatttat aatattttgg     1560

gtacgctcaa agcgctcaag attgctccat tctgattaca tgaacatgac ccctcgccgg     1620

ccgggtccca ctcgcaaaca ctaccaacct tatgcccctc cgcgggattt cgcggcctac     1680

aggtcagaga gagtacaacc tcttgaggag aatgtcggca acgctgccag gccgcggttt     1740

gagcgaaata agcgagtcaa gttttctcga agtgccgacg ctcctgccta tcagcaaggc     1800

cagaatcaac tgtataacga gcttaacttg ggccggaggg aggagtacga tgtgctggat     1860

aagcgcaggg gacgggaccc ggaaatgggc ggaaagccca ggcggaaaaa cccacaggaa     1920

ggactctaca atgaactcca aaaggacaaa atggcagagg cctattcaga gattgggatg     1980

aagggagaaa ggaggcgagg aaaagggcac gacggcttgt atcagggctt gtctacggcc     2040

actaaagaca cctatgatgc cctgcatatg caggcacttc cccccagagg aagtggggaa     2100

ggccgaggtt cattgctcac ctgtggcgat gtggaagaaa atccaggtcc gatgcgcata     2160

agtaagcctc atctgcggtc catttctata caatgttatc tgtgcttgct tttgaactcc     2220

cactttctta cggaagcagg cattcatgtg ttcattctgg gttgtttttc tgccgggctg     2280

cccaaaaccg aggccaactg ggtcaacgtg atcagcgacc tcaagaagat cgaggatttg     2340

attcaaagta tgcatataga cgccacactc tatactgagt ccgacgttca cccgagttgt     2400

aaagttacgg ctatgaagtg ctttttgttg gaactccagg tgatttccct tgaatccggc     2460

gatgcgagca tccacgatac ggtagagaat cttattattc tggcgaataa ttctctgtct     2520

tcaaatggga atgtaactga gagcggttgt aaagaatgcg aagaacttga agaaaagaat     2580

atcaaggaat ttcttcagag tttcgtgcat attgttcaaa tgttcatcaa cacatcctga     2640


<210>  50
<211>  694
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD19 CAR-WT-8 (with signal)

<400>  50

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 
    130                 135                 140                 


Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser 
145                 150                 155                 160 


Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly 
                165                 170                 175     


Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly 
            180                 185                 190         


Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser 
        195                 200                 205             


Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys 
    210                 215                 220                 


Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys 
225                 230                 235                 240 


His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly 
                245                 250                 255     


Thr Ser Val Thr Val Ser Ser Glu Pro Lys Ser Cys Asp Lys Thr His 
            260                 265                 270         


Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val 
        275                 280                 285             


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
    290                 295                 300                 


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
305                 310                 315                 320 


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
                325                 330                 335     


Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
            340                 345                 350         


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
        355                 360                 365             


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
    370                 375                 380                 


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
385                 390                 395                 400 


Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
                405                 410                 415     


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
            420                 425                 430         


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
        435                 440                 445             


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
    450                 455                 460                 


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
465                 470                 475                 480 


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Ile 
                485                 490                 495     


Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser 
            500                 505                 510         


Leu Val Ile Thr Leu Tyr Cys Arg Ser Lys Arg Ser Arg Leu Leu His 
        515                 520                 525             


Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys 
    530                 535                 540                 


His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
545                 550                 555                 560 


Glu Arg Val Gln Pro Leu Glu Glu Asn Val Gly Asn Ala Ala Arg Pro 
                565                 570                 575     


Arg Phe Glu Arg Asn Lys Arg Val Lys Phe Ser Arg Ser Ala Asp Ala 
            580                 585                 590         


Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu 
        595                 600                 605             


Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp 
    610                 615                 620                 


Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu 
625                 630                 635                 640 


Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile 
                645                 650                 655     


Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr 
            660                 665                 670         


Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met 
        675                 680                 685             


Gln Ala Leu Pro Pro Arg 
    690                 


<210>  51
<211>  2082
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD19 CAR-WT-8

<400>  51
atggcacttc ctgttacagc cctcctgctc ccactggctt tgctgctgca tgctgcacga       60

ccggacattc aaatgacaca aactacgagc tccctctccg cctccctcgg tgacagggtg      120

accatctcat gtcgggctag tcaggacata agcaaatact tgaactggta ccagcaaaag      180

ccagacggga ccgtgaagct gctcatctat cacactagcc gccttcactc tggggtcccc      240

tctcgcttta gcggttccgg ctctggtaca gactattccc ttactatttc aaatcttgag      300

caggaagata tcgctacata tttttgtcaa caggggaata ctcttccgta tacattcggg      360

ggcggaacaa aacttgaaat tacaggcggc ggtggttcag gaggtggtgg gagcggtggg      420

gggggatcag aagttaagct gcaagaatca gggcccggac tggtagcgcc cagtcaaagt      480

ctttctgtta cttgtacagt atccggggtg tcccttccag attacggggt atcttggatt      540

aggcaacctc ctcggaaagg attggagtgg ttgggtgtta tctggggcag tgaaaccact      600

tactataata gtgctcttaa aagtagactc accataataa aggataatag caagagccag      660

gtattcctga aaatgaacag cttgcaaacg gatgataccg ctatctacta ctgcgccaaa      720

cactattatt atggcggtag ttacgctatg gattattggg gccaaggaac gtccgtcacc      780

gttagtagcg agccaaagtc ttgcgacaag actcatacct gcccgccttg tcccgcacca      840

gagttgctgg gtggtcccag cgtgtttctg tttccgccca agcccaagga cacactcatg      900

atctctagga cacccgaggt tacatgcgtc gtggttgatg tttctcatga agaccccgaa      960

gtgaagttta actggtatgt cgacggagtt gaggtccata atgctaaaac aaaaccacgc     1020

gaggaacaat ataatagcac ctatcgggtc gttagtgtcc ttactgtact tcaccaggac     1080

tggctcaacg gaaaggaata taagtgcaaa gtcagcaata aagccttgcc agcgcccatc     1140

gaaaaaacta tcagcaaggc caagggccaa cccagagaac cccaagtgta cacactccct     1200

cctagccggg atgaattgac caaaaatcaa gtctctctca cttgtctggt aaagggattc     1260

tatccaagtg atattgctgt cgaatgggag agcaacgggc aaccggagaa caattacaaa     1320

acgacacctc ccgtccttga tagtgacggg tccttcttcc tgtattcaaa gcttacagtc     1380

gacaaaagtc gctggcagca ggggaatgtc tttagctgta gtgtcatgca cgaagctttg     1440

cataaccact acacgcaaaa gtctcttagc ctgtccccag gaaagatcta catttgggcc     1500

cctctggctg gaacatgtgg cgtgctgctg ctgtccctgg tcattactct gtattgtcgc     1560

tcaaagcgct caagattgct ccattctgat tacatgaaca tgacccctcg ccggccgggt     1620

cccactcgca aacactacca accttatgcc cctccgcggg atttcgcggc ctacaggtca     1680

gagagagtac aacctcttga ggagaatgtc ggcaacgctg ccaggccgcg gtttgagcga     1740

aataagcgag tcaagttttc tcgaagtgcc gacgctcctg cctatcagca aggccagaat     1800

caactgtata acgagcttaa cttgggccgg agggaggagt acgatgtgct ggataagcgc     1860

aggggacggg acccggaaat gggcggaaag cccaggcgga aaaacccaca ggaaggactc     1920

tacaatgaac tccaaaagga caaaatggca gaggcctatt cagagattgg gatgaaggga     1980

gaaaggaggc gaggaaaagg gcacgacggc ttgtatcagg gcttgtctac ggccactaaa     2040

gacacctatg atgccctgca tatgcaggca cttcccccca ga                        2082


<210>  52
<211>  877
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD19 CAR-WT-8 with T2A and IL-15 (with signal)

<400>  52

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 
    130                 135                 140                 


Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser 
145                 150                 155                 160 


Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly 
                165                 170                 175     


Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly 
            180                 185                 190         


Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser 
        195                 200                 205             


Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys 
    210                 215                 220                 


Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys 
225                 230                 235                 240 


His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly 
                245                 250                 255     


Thr Ser Val Thr Val Ser Ser Glu Pro Lys Ser Cys Asp Lys Thr His 
            260                 265                 270         


Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val 
        275                 280                 285             


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
    290                 295                 300                 


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
305                 310                 315                 320 


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
                325                 330                 335     


Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
            340                 345                 350         


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
        355                 360                 365             


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
    370                 375                 380                 


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
385                 390                 395                 400 


Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
                405                 410                 415     


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
            420                 425                 430         


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
        435                 440                 445             


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
    450                 455                 460                 


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
465                 470                 475                 480 


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Ile 
                485                 490                 495     


Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser 
            500                 505                 510         


Leu Val Ile Thr Leu Tyr Cys Arg Ser Lys Arg Ser Arg Leu Leu His 
        515                 520                 525             


Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys 
    530                 535                 540                 


His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
545                 550                 555                 560 


Glu Arg Val Gln Pro Leu Glu Glu Asn Val Gly Asn Ala Ala Arg Pro 
                565                 570                 575     


Arg Phe Glu Arg Asn Lys Arg Val Lys Phe Ser Arg Ser Ala Asp Ala 
            580                 585                 590         


Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu 
        595                 600                 605             


Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp 
    610                 615                 620                 


Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu 
625                 630                 635                 640 


Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile 
                645                 650                 655     


Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr 
            660                 665                 670         


Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met 
        675                 680                 685             


Gln Ala Leu Pro Pro Arg Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu 
    690                 695                 700                 


Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Arg Ile Ser Lys 
705                 710                 715                 720 


Pro His Leu Arg Ser Ile Ser Ile Gln Cys Tyr Leu Cys Leu Leu Leu 
                725                 730                 735     


Asn Ser His Phe Leu Thr Glu Ala Gly Ile His Val Phe Ile Leu Gly 
            740                 745                 750         


Cys Phe Ser Ala Gly Leu Pro Lys Thr Glu Ala Asn Trp Val Asn Val 
        755                 760                 765             


Ile Ser Asp Leu Lys Lys Ile Glu Asp Leu Ile Gln Ser Met His Ile 
    770                 775                 780                 


Asp Ala Thr Leu Tyr Thr Glu Ser Asp Val His Pro Ser Cys Lys Val 
785                 790                 795                 800 


Thr Ala Met Lys Cys Phe Leu Leu Glu Leu Gln Val Ile Ser Leu Glu 
                805                 810                 815     


Ser Gly Asp Ala Ser Ile His Asp Thr Val Glu Asn Leu Ile Ile Leu 
            820                 825                 830         


Ala Asn Asn Ser Leu Ser Ser Asn Gly Asn Val Thr Glu Ser Gly Cys 
        835                 840                 845             


Lys Glu Cys Glu Glu Leu Glu Glu Lys Asn Ile Lys Glu Phe Leu Gln 
    850                 855                 860                 


Ser Phe Val His Ile Val Gln Met Phe Ile Asn Thr Ser 
865                 870                 875         


<210>  53
<211>  2634
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD19 CAR-WT-8 with T2A and IL-15

<400>  53
atggcacttc ctgttacagc cctcctgctc ccactggctt tgctgctgca tgctgcacga       60

ccggacattc aaatgacaca aactacgagc tccctctccg cctccctcgg tgacagggtg      120

accatctcat gtcgggctag tcaggacata agcaaatact tgaactggta ccagcaaaag      180

ccagacggga ccgtgaagct gctcatctat cacactagcc gccttcactc tggggtcccc      240

tctcgcttta gcggttccgg ctctggtaca gactattccc ttactatttc aaatcttgag      300

caggaagata tcgctacata tttttgtcaa caggggaata ctcttccgta tacattcggg      360

ggcggaacaa aacttgaaat tacaggcggc ggtggttcag gaggtggtgg gagcggtggg      420

gggggatcag aagttaagct gcaagaatca gggcccggac tggtagcgcc cagtcaaagt      480

ctttctgtta cttgtacagt atccggggtg tcccttccag attacggggt atcttggatt      540

aggcaacctc ctcggaaagg attggagtgg ttgggtgtta tctggggcag tgaaaccact      600

tactataata gtgctcttaa aagtagactc accataataa aggataatag caagagccag      660

gtattcctga aaatgaacag cttgcaaacg gatgataccg ctatctacta ctgcgccaaa      720

cactattatt atggcggtag ttacgctatg gattattggg gccaaggaac gtccgtcacc      780

gttagtagcg agccaaagtc ttgcgacaag actcatacct gcccgccttg tcccgcacca      840

gagttgctgg gtggtcccag cgtgtttctg tttccgccca agcccaagga cacactcatg      900

atctctagga cacccgaggt tacatgcgtc gtggttgatg tttctcatga agaccccgaa      960

gtgaagttta actggtatgt cgacggagtt gaggtccata atgctaaaac aaaaccacgc     1020

gaggaacaat ataatagcac ctatcgggtc gttagtgtcc ttactgtact tcaccaggac     1080

tggctcaacg gaaaggaata taagtgcaaa gtcagcaata aagccttgcc agcgcccatc     1140

gaaaaaacta tcagcaaggc caagggccaa cccagagaac cccaagtgta cacactccct     1200

cctagccggg atgaattgac caaaaatcaa gtctctctca cttgtctggt aaagggattc     1260

tatccaagtg atattgctgt cgaatgggag agcaacgggc aaccggagaa caattacaaa     1320

acgacacctc ccgtccttga tagtgacggg tccttcttcc tgtattcaaa gcttacagtc     1380

gacaaaagtc gctggcagca ggggaatgtc tttagctgta gtgtcatgca cgaagctttg     1440

cataaccact acacgcaaaa gtctcttagc ctgtccccag gaaagatcta catttgggcc     1500

cctctggctg gaacatgtgg cgtgctgctg ctgtccctgg tcattactct gtattgtcgc     1560

tcaaagcgct caagattgct ccattctgat tacatgaaca tgacccctcg ccggccgggt     1620

cccactcgca aacactacca accttatgcc cctccgcggg atttcgcggc ctacaggtca     1680

gagagagtac aacctcttga ggagaatgtc ggcaacgctg ccaggccgcg gtttgagcga     1740

aataagcgag tcaagttttc tcgaagtgcc gacgctcctg cctatcagca aggccagaat     1800

caactgtata acgagcttaa cttgggccgg agggaggagt acgatgtgct ggataagcgc     1860

aggggacggg acccggaaat gggcggaaag cccaggcgga aaaacccaca ggaaggactc     1920

tacaatgaac tccaaaagga caaaatggca gaggcctatt cagagattgg gatgaaggga     1980

gaaaggaggc gaggaaaagg gcacgacggc ttgtatcagg gcttgtctac ggccactaaa     2040

gacacctatg atgccctgca tatgcaggca cttcccccca gaggaagtgg ggaaggccga     2100

ggttcattgc tcacctgtgg cgatgtggaa gaaaatccag gtccgatgcg cataagtaag     2160

cctcatctgc ggtccatttc tatacaatgt tatctgtgct tgcttttgaa ctcccacttt     2220

cttacggaag caggcattca tgtgttcatt ctgggttgtt tttctgccgg gctgcccaaa     2280

accgaggcca actgggtcaa cgtgatcagc gacctcaaga agatcgagga tttgattcaa     2340

agtatgcata tagacgccac actctatact gagtccgacg ttcacccgag ttgtaaagtt     2400

acggctatga agtgcttttt gttggaactc caggtgattt cccttgaatc cggcgatgcg     2460

agcatccacg atacggtaga gaatcttatt attctggcga ataattctct gtcttcaaat     2520

gggaatgtaa ctgagagcgg ttgtaaagaa tgcgaagaac ttgaagaaaa gaatatcaag     2580

gaatttcttc agagtttcgt gcatattgtt caaatgttca tcaacacatc ctga           2634


<210>  54
<211>  693
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD19 CAR-MU-8 (with signal)

<400>  54

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 
    130                 135                 140                 


Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser 
145                 150                 155                 160 


Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly 
                165                 170                 175     


Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly 
            180                 185                 190         


Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser 
        195                 200                 205             


Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys 
    210                 215                 220                 


Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys 
225                 230                 235                 240 


His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly 
                245                 250                 255     


Thr Ser Val Thr Val Ser Ser Glu Pro Lys Ser Cys Asp Lys Thr His 
            260                 265                 270         


Thr Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser Val Phe 
        275                 280                 285             


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
    290                 295                 300                 


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
305                 310                 315                 320 


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
                325                 330                 335     


Lys Pro Arg Glu Glu Gln Tyr Gln Ser Thr Tyr Arg Val Val Ser Val 
            340                 345                 350         


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
        355                 360                 365             


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
    370                 375                 380                 


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
385                 390                 395                 400 


Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
                405                 410                 415     


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
            420                 425                 430         


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
        435                 440                 445             


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
    450                 455                 460                 


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
465                 470                 475                 480 


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Ile Tyr 
                485                 490                 495     


Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu 
            500                 505                 510         


Val Ile Thr Leu Tyr Cys Arg Ser Lys Arg Ser Arg Leu Leu His Ser 
        515                 520                 525             


Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His 
    530                 535                 540                 


Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Glu 
545                 550                 555                 560 


Arg Val Gln Pro Leu Glu Glu Asn Val Gly Asn Ala Ala Arg Pro Arg 
                565                 570                 575     


Phe Glu Arg Asn Lys Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro 
            580                 585                 590         


Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly 
        595                 600                 605             


Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro 
    610                 615                 620                 


Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr 
625                 630                 635                 640 


Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly 
                645                 650                 655     


Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln 
            660                 665                 670         


Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln 
        675                 680                 685             


Ala Leu Pro Pro Arg 
    690             


<210>  55
<211>  2079
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD19 CAR-MU-8

<400>  55
atggcacttc ctgttacagc cctcctgctc ccactggctt tgctgctgca tgctgcacga       60

ccggacattc aaatgacaca aactacgagc tccctctccg cctccctcgg tgacagggtg      120

accatctcat gtcgggctag tcaggacata agcaaatact tgaactggta ccagcaaaag      180

ccagacggga ccgtgaagct gctcatctat cacactagcc gccttcactc tggggtcccc      240

tctcgcttta gcggttccgg ctctggtaca gactattccc ttactatttc aaatcttgag      300

caggaagata tcgctacata tttttgtcaa caggggaata ctcttccgta tacattcggg      360

ggcggaacaa aacttgaaat tacaggcggc ggtggttcag gaggtggtgg gagcggtggg      420

gggggatcag aagttaagct gcaagaatca gggcccggac tggtagcgcc cagtcaaagt      480

ctttctgtta cttgtacagt atccggggtg tcccttccag attacggggt atcttggatt      540

aggcaacctc ctcggaaagg attggagtgg ttgggtgtta tctggggcag tgaaaccact      600

tactataata gtgctcttaa aagtagactc accataataa aggataatag caagagccag      660

gtattcctga aaatgaacag cttgcaaacg gatgataccg ctatctacta ctgcgccaaa      720

cactattatt atggcggtag ttacgctatg gattattggg gccaaggaac gtccgtcacc      780

gttagtagcg agccaaagtc ttgcgacaag actcatacct gcccgccttg tcccgcacca      840

cctgtggcag gtcccagcgt gtttctgttt ccgcccaagc ccaaggacac actcatgatc      900

tctaggacac ccgaggttac atgcgtcgtg gttgatgttt ctcatgaaga ccccgaagtg      960

aagtttaact ggtatgtcga cggagttgag gtccataatg ctaaaacaaa accacgcgag     1020

gaacaatatc aaagcaccta tcgggtcgtt agtgtcctta ctgtacttca ccaggactgg     1080

ctcaacggaa aggaatataa gtgcaaagtc agcaataaag ccttgccagc gcccatcgaa     1140

aaaactatca gcaaggccaa gggccaaccc agagaacccc aagtgtacac actccctcct     1200

agccgggatg aattgaccaa aaatcaagtc tctctcactt gtctggtaaa gggattctat     1260

ccaagtgata ttgctgtcga atgggagagc aacgggcaac cggagaacaa ttacaaaacg     1320

acacctcccg tccttgatag tgacgggtcc ttcttcctgt attcaaagct tacagtcgac     1380

aaaagtcgct ggcagcaggg gaatgtcttt agctgtagtg tcatgcacga agctttgcat     1440

aaccactaca cgcaaaagtc tcttagcctg tccccaggaa agatctacat ttgggcccct     1500

ctggctggaa catgtggcgt gctgctgctg tccctggtca ttactctgta ttgtcgctca     1560

aagcgctcaa gattgctcca ttctgattac atgaacatga cccctcgccg gccgggtccc     1620

actcgcaaac actaccaacc ttatgcccct ccgcgggatt tcgcggccta caggtcagag     1680

agagtacaac ctcttgagga gaatgtcggc aacgctgcca ggccgcggtt tgagcgaaat     1740

aagcgagtca agttttctcg aagtgccgac gctcctgcct atcagcaagg ccagaatcaa     1800

ctgtataacg agcttaactt gggccggagg gaggagtacg atgtgctgga taagcgcagg     1860

ggacgggacc cggaaatggg cggaaagccc aggcggaaaa acccacagga aggactctac     1920

aatgaactcc aaaaggacaa aatggcagag gcctattcag agattgggat gaagggagaa     1980

aggaggcgag gaaaagggca cgacggcttg tatcagggct tgtctacggc cactaaagac     2040

acctatgatg ccctgcatat gcaggcactt ccccccaga                            2079


<210>  56
<211>  876
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD19 CAR-MU-8 with T2A and IL-15

<400>  56

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 
    130                 135                 140                 


Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser 
145                 150                 155                 160 


Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly 
                165                 170                 175     


Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly 
            180                 185                 190         


Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser 
        195                 200                 205             


Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys 
    210                 215                 220                 


Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys 
225                 230                 235                 240 


His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly 
                245                 250                 255     


Thr Ser Val Thr Val Ser Ser Glu Pro Lys Ser Cys Asp Lys Thr His 
            260                 265                 270         


Thr Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser Val Phe 
        275                 280                 285             


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
    290                 295                 300                 


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
305                 310                 315                 320 


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
                325                 330                 335     


Lys Pro Arg Glu Glu Gln Tyr Gln Ser Thr Tyr Arg Val Val Ser Val 
            340                 345                 350         


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
        355                 360                 365             


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
    370                 375                 380                 


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
385                 390                 395                 400 


Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
                405                 410                 415     


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
            420                 425                 430         


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
        435                 440                 445             


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
    450                 455                 460                 


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
465                 470                 475                 480 


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Ile Tyr 
                485                 490                 495     


Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu 
            500                 505                 510         


Val Ile Thr Leu Tyr Cys Arg Ser Lys Arg Ser Arg Leu Leu His Ser 
        515                 520                 525             


Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His 
    530                 535                 540                 


Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Glu 
545                 550                 555                 560 


Arg Val Gln Pro Leu Glu Glu Asn Val Gly Asn Ala Ala Arg Pro Arg 
                565                 570                 575     


Phe Glu Arg Asn Lys Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro 
            580                 585                 590         


Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly 
        595                 600                 605             


Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro 
    610                 615                 620                 


Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr 
625                 630                 635                 640 


Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly 
                645                 650                 655     


Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln 
            660                 665                 670         


Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln 
        675                 680                 685             


Ala Leu Pro Pro Arg Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr 
    690                 695                 700                 


Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Arg Ile Ser Lys Pro 
705                 710                 715                 720 


His Leu Arg Ser Ile Ser Ile Gln Cys Tyr Leu Cys Leu Leu Leu Asn 
                725                 730                 735     


Ser His Phe Leu Thr Glu Ala Gly Ile His Val Phe Ile Leu Gly Cys 
            740                 745                 750         


Phe Ser Ala Gly Leu Pro Lys Thr Glu Ala Asn Trp Val Asn Val Ile 
        755                 760                 765             


Ser Asp Leu Lys Lys Ile Glu Asp Leu Ile Gln Ser Met His Ile Asp 
    770                 775                 780                 


Ala Thr Leu Tyr Thr Glu Ser Asp Val His Pro Ser Cys Lys Val Thr 
785                 790                 795                 800 


Ala Met Lys Cys Phe Leu Leu Glu Leu Gln Val Ile Ser Leu Glu Ser 
                805                 810                 815     


Gly Asp Ala Ser Ile His Asp Thr Val Glu Asn Leu Ile Ile Leu Ala 
            820                 825                 830         


Asn Asn Ser Leu Ser Ser Asn Gly Asn Val Thr Glu Ser Gly Cys Lys 
        835                 840                 845             


Glu Cys Glu Glu Leu Glu Glu Lys Asn Ile Lys Glu Phe Leu Gln Ser 
    850                 855                 860                 


Phe Val His Ile Val Gln Met Phe Ile Asn Thr Ser 
865                 870                 875     


<210>  57
<211>  2631
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD19 CAR-MU-8 with T2A and IL-15

<400>  57
atggcacttc ctgttacagc cctcctgctc ccactggctt tgctgctgca tgctgcacga       60

ccggacattc aaatgacaca aactacgagc tccctctccg cctccctcgg tgacagggtg      120

accatctcat gtcgggctag tcaggacata agcaaatact tgaactggta ccagcaaaag      180

ccagacggga ccgtgaagct gctcatctat cacactagcc gccttcactc tggggtcccc      240

tctcgcttta gcggttccgg ctctggtaca gactattccc ttactatttc aaatcttgag      300

caggaagata tcgctacata tttttgtcaa caggggaata ctcttccgta tacattcggg      360

ggcggaacaa aacttgaaat tacaggcggc ggtggttcag gaggtggtgg gagcggtggg      420

gggggatcag aagttaagct gcaagaatca gggcccggac tggtagcgcc cagtcaaagt      480

ctttctgtta cttgtacagt atccggggtg tcccttccag attacggggt atcttggatt      540

aggcaacctc ctcggaaagg attggagtgg ttgggtgtta tctggggcag tgaaaccact      600

tactataata gtgctcttaa aagtagactc accataataa aggataatag caagagccag      660

gtattcctga aaatgaacag cttgcaaacg gatgataccg ctatctacta ctgcgccaaa      720

cactattatt atggcggtag ttacgctatg gattattggg gccaaggaac gtccgtcacc      780

gttagtagcg agccaaagtc ttgcgacaag actcatacct gcccgccttg tcccgcacca      840

cctgtggcag gtcccagcgt gtttctgttt ccgcccaagc ccaaggacac actcatgatc      900

tctaggacac ccgaggttac atgcgtcgtg gttgatgttt ctcatgaaga ccccgaagtg      960

aagtttaact ggtatgtcga cggagttgag gtccataatg ctaaaacaaa accacgcgag     1020

gaacaatatc aaagcaccta tcgggtcgtt agtgtcctta ctgtacttca ccaggactgg     1080

ctcaacggaa aggaatataa gtgcaaagtc agcaataaag ccttgccagc gcccatcgaa     1140

aaaactatca gcaaggccaa gggccaaccc agagaacccc aagtgtacac actccctcct     1200

agccgggatg aattgaccaa aaatcaagtc tctctcactt gtctggtaaa gggattctat     1260

ccaagtgata ttgctgtcga atgggagagc aacgggcaac cggagaacaa ttacaaaacg     1320

acacctcccg tccttgatag tgacgggtcc ttcttcctgt attcaaagct tacagtcgac     1380

aaaagtcgct ggcagcaggg gaatgtcttt agctgtagtg tcatgcacga agctttgcat     1440

aaccactaca cgcaaaagtc tcttagcctg tccccaggaa agatctacat ttgggcccct     1500

ctggctggaa catgtggcgt gctgctgctg tccctggtca ttactctgta ttgtcgctca     1560

aagcgctcaa gattgctcca ttctgattac atgaacatga cccctcgccg gccgggtccc     1620

actcgcaaac actaccaacc ttatgcccct ccgcgggatt tcgcggccta caggtcagag     1680

agagtacaac ctcttgagga gaatgtcggc aacgctgcca ggccgcggtt tgagcgaaat     1740

aagcgagtca agttttctcg aagtgccgac gctcctgcct atcagcaagg ccagaatcaa     1800

ctgtataacg agcttaactt gggccggagg gaggagtacg atgtgctgga taagcgcagg     1860

ggacgggacc cggaaatggg cggaaagccc aggcggaaaa acccacagga aggactctac     1920

aatgaactcc aaaaggacaa aatggcagag gcctattcag agattgggat gaagggagaa     1980

aggaggcgag gaaaagggca cgacggcttg tatcagggct tgtctacggc cactaaagac     2040

acctatgatg ccctgcatat gcaggcactt ccccccagag gaagtgggga aggccgaggt     2100

tcattgctca cctgtggcga tgtggaagaa aatccaggtc cgatgcgcat aagtaagcct     2160

catctgcggt ccatttctat acaatgttat ctgtgcttgc ttttgaactc ccactttctt     2220

acggaagcag gcattcatgt gttcattctg ggttgttttt ctgccgggct gcccaaaacc     2280

gaggccaact gggtcaacgt gatcagcgac ctcaagaaga tcgaggattt gattcaaagt     2340

atgcatatag acgccacact ctatactgag tccgacgttc acccgagttg taaagttacg     2400

gctatgaagt gctttttgtt ggaactccag gtgatttccc ttgaatccgg cgatgcgagc     2460

atccacgata cggtagagaa tcttattatt ctggcgaata attctctgtc ttcaaatggg     2520

aatgtaactg agagcggttg taaagaatgc gaagaacttg aagaaaagaa tatcaaggaa     2580

tttcttcaga gtttcgtgca tattgttcaa atgttcatca acacatcctg a              2631


<210>  58
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD19 scFv  CDRL1

<400>  58

Arg Ala Ser Gln Asp Ile Ser Lys Tyr Leu 
1               5                   10  


<210>  59
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD19 scFv  CDRL2

<400>  59

His Thr Ser Arg Leu His Ser 
1               5           


<210>  60
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD19 scFv  CDRL3

<400>  60

Gln Gln Gly Asn Thr Leu Pro Tyr Thr 
1               5                   


<210>  61
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD19 scFv  CDRH1

<400>  61

Asp Tyr Gly Val Ser 
1               5   


<210>  62
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD19 scFv  CDRH2

<400>  62

Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser 
1               5                   10                  15      


<210>  63
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD19 scFv  CDRH3

<400>  63

His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr 
1               5                   10          


<210>  64
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD19 scFv  VL

<400>  64

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
            100                 105         


<210>  65
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD19 scFv  VH

<400>  65

Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln 
1               5                   10                  15      


Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr 
            20                  25                  30          


Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys 
    50                  55                  60                  


Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu 
65                  70                  75                  80  


Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala 
                85                  90                  95      


Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Ser Val Thr Val Ser Ser 
        115                 120 


<210>  66
<211>  556
<212>  PRT
<213>  Homo sapiens

<400>  66

Met Pro Pro Pro Arg Leu Leu Phe Phe Leu Leu Phe Leu Thr Pro Met 
1               5                   10                  15      


Glu Val Arg Pro Glu Glu Pro Leu Val Val Lys Val Glu Glu Gly Asp 
            20                  25                  30          


Asn Ala Val Leu Gln Cys Leu Lys Gly Thr Ser Asp Gly Pro Thr Gln 
        35                  40                  45              


Gln Leu Thr Trp Ser Arg Glu Ser Pro Leu Lys Pro Phe Leu Lys Leu 
    50                  55                  60                  


Ser Leu Gly Leu Pro Gly Leu Gly Ile His Met Arg Pro Leu Ala Ile 
65                  70                  75                  80  


Trp Leu Phe Ile Phe Asn Val Ser Gln Gln Met Gly Gly Phe Tyr Leu 
                85                  90                  95      


Cys Gln Pro Gly Pro Pro Ser Glu Lys Ala Trp Gln Pro Gly Trp Thr 
            100                 105                 110         


Val Asn Val Glu Gly Ser Gly Glu Leu Phe Arg Trp Asn Val Ser Asp 
        115                 120                 125             


Leu Gly Gly Leu Gly Cys Gly Leu Lys Asn Arg Ser Ser Glu Gly Pro 
    130                 135                 140                 


Ser Ser Pro Ser Gly Lys Leu Met Ser Pro Lys Leu Tyr Val Trp Ala 
145                 150                 155                 160 


Lys Asp Arg Pro Glu Ile Trp Glu Gly Glu Pro Pro Cys Leu Pro Pro 
                165                 170                 175     


Arg Asp Ser Leu Asn Gln Ser Leu Ser Gln Asp Leu Thr Met Ala Pro 
            180                 185                 190         


Gly Ser Thr Leu Trp Leu Ser Cys Gly Val Pro Pro Asp Ser Val Ser 
        195                 200                 205             


Arg Gly Pro Leu Ser Trp Thr His Val His Pro Lys Gly Pro Lys Ser 
    210                 215                 220                 


Leu Leu Ser Leu Glu Leu Lys Asp Asp Arg Pro Ala Arg Asp Met Trp 
225                 230                 235                 240 


Val Met Glu Thr Gly Leu Leu Leu Pro Arg Ala Thr Ala Gln Asp Ala 
                245                 250                 255     


Gly Lys Tyr Tyr Cys His Arg Gly Asn Leu Thr Met Ser Phe His Leu 
            260                 265                 270         


Glu Ile Thr Ala Arg Pro Val Leu Trp His Trp Leu Leu Arg Thr Gly 
        275                 280                 285             


Gly Trp Lys Val Ser Ala Val Thr Leu Ala Tyr Leu Ile Phe Cys Leu 
    290                 295                 300                 


Cys Ser Leu Val Gly Ile Leu His Leu Gln Arg Ala Leu Val Leu Arg 
305                 310                 315                 320 


Arg Lys Arg Lys Arg Met Thr Asp Pro Thr Arg Arg Phe Phe Lys Val 
                325                 330                 335     


Thr Pro Pro Pro Gly Ser Gly Pro Gln Asn Gln Tyr Gly Asn Val Leu 
            340                 345                 350         


Ser Leu Pro Thr Pro Thr Ser Gly Leu Gly Arg Ala Gln Arg Trp Ala 
        355                 360                 365             


Ala Gly Leu Gly Gly Thr Ala Pro Ser Tyr Gly Asn Pro Ser Ser Asp 
    370                 375                 380                 


Val Gln Ala Asp Gly Ala Leu Gly Ser Arg Ser Pro Pro Gly Val Gly 
385                 390                 395                 400 


Pro Glu Glu Glu Glu Gly Glu Gly Tyr Glu Glu Pro Asp Ser Glu Glu 
                405                 410                 415     


Asp Ser Glu Phe Tyr Glu Asn Asp Ser Asn Leu Gly Gln Asp Gln Leu 
            420                 425                 430         


Ser Gln Asp Gly Ser Gly Tyr Glu Asn Pro Glu Asp Glu Pro Leu Gly 
        435                 440                 445             


Pro Glu Asp Glu Asp Ser Phe Ser Asn Ala Glu Ser Tyr Glu Asn Glu 
    450                 455                 460                 


Asp Glu Glu Leu Thr Gln Pro Val Ala Arg Thr Met Asp Phe Leu Ser 
465                 470                 475                 480 


Pro His Gly Ser Ala Trp Asp Pro Ser Arg Glu Ala Thr Ser Leu Gly 
                485                 490                 495     


Ser Gln Ser Tyr Glu Asp Met Arg Gly Ile Leu Tyr Ala Ala Pro Gln 
            500                 505                 510         


Leu Arg Ser Ile Arg Gly Gln Pro Gly Pro Asn His Glu Glu Asp Ala 
        515                 520                 525             


Asp Ser Tyr Glu Asn Met Asp Asn Pro Asp Gly Pro Asp Pro Ala Trp 
    530                 535                 540                 


Gly Gly Gly Gly Arg Met Gly Thr Trp Ser Thr Arg 
545                 550                 555     


<210>  67
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer

<400>  67

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  68
<211>  609
<212>  PRT
<213>  Homo sapiens

<400>  68

Met Lys Trp Val Thr Phe Ile Ser Leu Leu Phe Leu Phe Ser Ser Ala 
1               5                   10                  15      


Tyr Ser Arg Gly Val Phe Arg Arg Asp Ala His Lys Ser Glu Val Ala 
            20                  25                  30          


His Arg Phe Lys Asp Leu Gly Glu Glu Asn Phe Lys Ala Leu Val Leu 
        35                  40                  45              


Ile Ala Phe Ala Gln Tyr Leu Gln Gln Cys Pro Phe Glu Asp His Val 
    50                  55                  60                  


Lys Leu Val Asn Glu Val Thr Glu Phe Ala Lys Thr Cys Val Ala Asp 
65                  70                  75                  80  


Glu Ser Ala Glu Asn Cys Asp Lys Ser Leu His Thr Leu Phe Gly Asp 
                85                  90                  95      


Lys Leu Cys Thr Val Ala Thr Leu Arg Glu Thr Tyr Gly Glu Met Ala 
            100                 105                 110         


Asp Cys Cys Ala Lys Gln Glu Pro Glu Arg Asn Glu Cys Phe Leu Gln 
        115                 120                 125             


His Lys Asp Asp Asn Pro Asn Leu Pro Arg Leu Val Arg Pro Glu Val 
    130                 135                 140                 


Asp Val Met Cys Thr Ala Phe His Asp Asn Glu Glu Thr Phe Leu Lys 
145                 150                 155                 160 


Lys Tyr Leu Tyr Glu Ile Ala Arg Arg His Pro Tyr Phe Tyr Ala Pro 
                165                 170                 175     


Glu Leu Leu Phe Phe Ala Lys Arg Tyr Lys Ala Ala Phe Thr Glu Cys 
            180                 185                 190         


Cys Gln Ala Ala Asp Lys Ala Ala Cys Leu Leu Pro Lys Leu Asp Glu 
        195                 200                 205             


Leu Arg Asp Glu Gly Lys Ala Ser Ser Ala Lys Gln Arg Leu Lys Cys 
    210                 215                 220                 


Ala Ser Leu Gln Lys Phe Gly Glu Arg Ala Phe Lys Ala Trp Ala Val 
225                 230                 235                 240 


Ala Arg Leu Ser Gln Arg Phe Pro Lys Ala Glu Phe Ala Glu Val Ser 
                245                 250                 255     


Lys Leu Val Thr Asp Leu Thr Lys Val His Thr Glu Cys Cys His Gly 
            260                 265                 270         


Asp Leu Leu Glu Cys Ala Asp Asp Arg Ala Asp Leu Ala Lys Tyr Ile 
        275                 280                 285             


Cys Glu Asn Gln Asp Ser Ile Ser Ser Lys Leu Lys Glu Cys Cys Glu 
    290                 295                 300                 


Lys Pro Leu Leu Glu Lys Ser His Cys Ile Ala Glu Val Glu Asn Asp 
305                 310                 315                 320 


Glu Met Pro Ala Asp Leu Pro Ser Leu Ala Ala Asp Phe Val Glu Ser 
                325                 330                 335     


Lys Asp Val Cys Lys Asn Tyr Ala Glu Ala Lys Asp Val Phe Leu Gly 
            340                 345                 350         


Met Phe Leu Tyr Glu Tyr Ala Arg Arg His Pro Asp Tyr Ser Val Val 
        355                 360                 365             


Leu Leu Leu Arg Leu Ala Lys Thr Tyr Glu Thr Thr Leu Glu Lys Cys 
    370                 375                 380                 


Cys Ala Ala Ala Asp Pro His Glu Cys Tyr Ala Lys Val Phe Asp Glu 
385                 390                 395                 400 


Phe Lys Pro Leu Val Glu Glu Pro Gln Asn Leu Ile Lys Gln Asn Cys 
                405                 410                 415     


Glu Leu Phe Glu Gln Leu Gly Glu Tyr Lys Phe Gln Asn Ala Leu Leu 
            420                 425                 430         


Val Arg Tyr Thr Lys Lys Val Pro Gln Val Ser Thr Pro Thr Leu Val 
        435                 440                 445             


Glu Val Ser Arg Asn Leu Gly Lys Val Gly Ser Lys Cys Cys Lys His 
    450                 455                 460                 


Pro Glu Ala Lys Arg Met Pro Cys Ala Glu Asp Tyr Leu Ser Val Val 
465                 470                 475                 480 


Leu Asn Gln Leu Cys Val Leu His Glu Lys Thr Pro Val Ser Asp Arg 
                485                 490                 495     


Val Thr Lys Cys Cys Thr Glu Ser Leu Val Asn Arg Arg Pro Cys Phe 
            500                 505                 510         


Ser Ala Leu Glu Val Asp Glu Thr Tyr Val Pro Lys Glu Phe Asn Ala 
        515                 520                 525             


Glu Thr Phe Thr Phe His Ala Asp Ile Cys Thr Leu Ser Glu Lys Glu 
    530                 535                 540                 


Arg Gln Ile Lys Lys Gln Thr Ala Leu Val Glu Leu Val Lys His Lys 
545                 550                 555                 560 


Pro Lys Ala Thr Lys Glu Gln Leu Lys Ala Val Met Asp Asp Phe Ala 
                565                 570                 575     


Ala Phe Val Glu Lys Cys Cys Lys Ala Asp Asp Lys Glu Thr Cys Phe 
            580                 585                 590         


Ala Glu Glu Gly Lys Lys Leu Val Ala Ala Ser Gln Ala Ala Leu Gly 
        595                 600                 605             


Leu 
    


<210>  69
<211>  676
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD19 CAR-WT-28

<400>  69

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Gly Gly Gly Ser 
            100                 105                 110         


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Gln Glu 
        115                 120                 125             


Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys 
    130                 135                 140                 


Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg 
145                 150                 155                 160 


Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser 
                165                 170                 175     


Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile 
            180                 185                 190         


Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln 
        195                 200                 205             


Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly 
    210                 215                 220                 


Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val 
225                 230                 235                 240 


Ser Ser Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
            260                 265                 270         


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
        275                 280                 285             


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
    290                 295                 300                 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
305                 310                 315                 320 


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
                325                 330                 335     


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
            340                 345                 350         


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
        355                 360                 365             


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
    370                 375                 380                 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
385                 390                 395                 400 


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
                405                 410                 415     


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
            420                 425                 430         


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
        435                 440                 445             


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
    450                 455                 460                 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Phe Trp Val Leu Val Val 
465                 470                 475                 480 


Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe 
                485                 490                 495     


Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp 
            500                 505                 510         


Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr 
        515                 520                 525             


Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Glu Arg 
    530                 535                 540                 


Val Gln Pro Leu Glu Glu Asn Val Gly Asn Ala Ala Arg Pro Arg Phe 
545                 550                 555                 560 


Glu Arg Asn Lys Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala 
                565                 570                 575     


Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg 
            580                 585                 590         


Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu 
        595                 600                 605             


Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
    610                 615                 620                 


Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
625                 630                 635                 640 


Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
                645                 650                 655     


Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
            660                 665                 670         


Leu Pro Pro Arg 
        675     


<210>  70
<211>  675
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD19 CAR-MU-28

<400>  70

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Gly Gly Gly Ser 
            100                 105                 110         


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Gln Glu 
        115                 120                 125             


Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys 
    130                 135                 140                 


Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg 
145                 150                 155                 160 


Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser 
                165                 170                 175     


Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile 
            180                 185                 190         


Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln 
        195                 200                 205             


Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly 
    210                 215                 220                 


Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val 
225                 230                 235                 240 


Ser Ser Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
            260                 265                 270         


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
        275                 280                 285             


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
    290                 295                 300                 


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
305                 310                 315                 320 


Gln Tyr Gln Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
                325                 330                 335     


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
            340                 345                 350         


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
        355                 360                 365             


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu 
    370                 375                 380                 


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
385                 390                 395                 400 


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
                405                 410                 415     


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
            420                 425                 430         


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
        435                 440                 445             


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
    450                 455                 460                 


Lys Ser Leu Ser Leu Ser Pro Gly Lys Phe Trp Val Leu Val Val Val 
465                 470                 475                 480 


Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile 
                485                 490                 495     


Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr 
            500                 505                 510         


Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln 
        515                 520                 525             


Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Glu Arg Val 
    530                 535                 540                 


Gln Pro Leu Glu Glu Asn Val Gly Asn Ala Ala Arg Pro Arg Phe Glu 
545                 550                 555                 560 


Arg Asn Lys Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
                565                 570                 575     


Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
            580                 585                 590         


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
        595                 600                 605             


Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
    610                 615                 620                 


Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
625                 630                 635                 640 


Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu 
                645                 650                 655     


Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu 
            660                 665                 670         


Pro Pro Arg 
        675 


<210>  71
<211>  673
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD19 CAR-WT-8

<400>  71

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Gly Gly Gly Ser 
            100                 105                 110         


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Gln Glu 
        115                 120                 125             


Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys 
    130                 135                 140                 


Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg 
145                 150                 155                 160 


Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser 
                165                 170                 175     


Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile 
            180                 185                 190         


Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln 
        195                 200                 205             


Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly 
    210                 215                 220                 


Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val 
225                 230                 235                 240 


Ser Ser Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
            260                 265                 270         


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
        275                 280                 285             


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
    290                 295                 300                 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
305                 310                 315                 320 


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
                325                 330                 335     


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
            340                 345                 350         


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
        355                 360                 365             


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
    370                 375                 380                 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
385                 390                 395                 400 


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
                405                 410                 415     


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
            420                 425                 430         


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
        435                 440                 445             


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
    450                 455                 460                 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Ile Tyr Ile Trp Ala Pro 
465                 470                 475                 480 


Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu 
                485                 490                 495     


Tyr Cys Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn 
            500                 505                 510         


Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr 
        515                 520                 525             


Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Glu Arg Val Gln Pro 
    530                 535                 540                 


Leu Glu Glu Asn Val Gly Asn Ala Ala Arg Pro Arg Phe Glu Arg Asn 
545                 550                 555                 560 


Lys Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln 
                565                 570                 575     


Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
            580                 585                 590         


Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
        595                 600                 605             


Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
    610                 615                 620                 


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
625                 630                 635                 640 


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
                645                 650                 655     


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
            660                 665                 670         


Arg 
    


<210>  72
<211>  672
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD19 CAR-MU-8

<400>  72

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Gly Gly Gly Ser 
            100                 105                 110         


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Gln Glu 
        115                 120                 125             


Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys 
    130                 135                 140                 


Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg 
145                 150                 155                 160 


Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser 
                165                 170                 175     


Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile 
            180                 185                 190         


Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln 
        195                 200                 205             


Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly 
    210                 215                 220                 


Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val 
225                 230                 235                 240 


Ser Ser Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
            260                 265                 270         


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
        275                 280                 285             


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
    290                 295                 300                 


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
305                 310                 315                 320 


Gln Tyr Gln Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
                325                 330                 335     


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
            340                 345                 350         


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
        355                 360                 365             


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu 
    370                 375                 380                 


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
385                 390                 395                 400 


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
                405                 410                 415     


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
            420                 425                 430         


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
        435                 440                 445             


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
    450                 455                 460                 


Lys Ser Leu Ser Leu Ser Pro Gly Lys Ile Tyr Ile Trp Ala Pro Leu 
465                 470                 475                 480 


Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr 
                485                 490                 495     


Cys Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met 
            500                 505                 510         


Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala 
        515                 520                 525             


Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Glu Arg Val Gln Pro Leu 
    530                 535                 540                 


Glu Glu Asn Val Gly Asn Ala Ala Arg Pro Arg Phe Glu Arg Asn Lys 
545                 550                 555                 560 


Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
                565                 570                 575     


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            580                 585                 590         


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        595                 600                 605             


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    610                 615                 620                 


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
625                 630                 635                 640 


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                645                 650                 655     


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            660                 665                 670         


<210>  73
<211>  653
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-CD19 CAR (CD28-CD3z-IL15)

<400>  73

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Gly Gly Gly Ser 
            100                 105                 110         


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Gln Glu 
        115                 120                 125             


Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys 
    130                 135                 140                 


Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg 
145                 150                 155                 160 


Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser 
                165                 170                 175     


Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile 
            180                 185                 190         


Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln 
        195                 200                 205             


Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly 
    210                 215                 220                 


Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val 
225                 230                 235                 240 


Ser Ser Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
            260                 265                 270         


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
        275                 280                 285             


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
    290                 295                 300                 


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
305                 310                 315                 320 


Gln Tyr Gln Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
                325                 330                 335     


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
            340                 345                 350         


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
        355                 360                 365             


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu 
    370                 375                 380                 


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
385                 390                 395                 400 


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
                405                 410                 415     


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
            420                 425                 430         


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
        435                 440                 445             


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
    450                 455                 460                 


Lys Ser Leu Ser Leu Ser Pro Gly Lys Phe Trp Val Leu Val Val Val 
465                 470                 475                 480 


Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile 
                485                 490                 495     


Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr 
            500                 505                 510         


Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln 
        515                 520                 525             


Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys 
    530                 535                 540                 


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
545                 550                 555                 560 


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
                565                 570                 575     


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
            580                 585                 590         


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
        595                 600                 605             


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
    610                 615                 620                 


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
625                 630                 635                 640 


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                645                 650             


<210>  74
<211>  1959
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anti-CD19 CAR (CD28-CD3z-IL15)

<400>  74
gacattcaaa tgacacaaac tacgagctcc ctctccgcct ccctcggtga cagggtgacc       60

atctcatgtc gggctagtca ggacataagc aaatacttga actggtacca gcaaaagcca      120

gacgggaccg tgaagctgct catctatcac actagccgcc ttcactctgg ggtcccctct      180

cgctttagcg gttccggctc tggtacagac tattccctta ctatttcaaa tcttgagcag      240

gaagatatcg ctacatattt ttgtcaacag gggaatactc ttccgtatac attcgggggc      300

ggaacaaaac ttgaaattac aggcggcggt ggttcaggag gtggtgggag cggtgggggg      360

ggatcagaag ttaagctgca agaatcaggg cccggactgg tagcgcccag tcaaagtctt      420

tctgttactt gtacagtatc cggggtgtcc cttccagatt acggggtatc ttggattagg      480

caacctcctc ggaaaggatt ggagtggttg ggtgttatct ggggcagtga aaccacttac      540

tataatagtg ctcttaaaag tagactcacc ataataaagg ataatagcaa gagccaggta      600

ttcctgaaaa tgaacagctt gcaaacggat gataccgcta tctactactg cgccaaacac      660

tattattatg gcggtagtta cgctatggat tattggggcc aaggaacgtc cgtcaccgtt      720

agtagcgagc caaagtcttg cgacaagact catacctgcc cgccttgtcc cgcaccacct      780

gtggcaggtc ccagcgtgtt tctgtttccg cccaagccca aggacacact catgatctct      840

aggacacccg aggttacatg cgtcgtggtt gatgtttctc atgaagaccc cgaagtgaag      900

tttaactggt atgtcgacgg agttgaggtc cataatgcta aaacaaaacc acgcgaggaa      960

caatatcaaa gcacctatcg ggtcgttagt gtccttactg tacttcacca ggactggctc     1020

aacggaaagg aatataagtg caaagtcagc aataaagcct tgccagcgcc catcgaaaaa     1080

actatcagca aggccaaggg ccaacccaga gaaccccaag tgtacacact ccctcctagc     1140

cgggatgaat tgaccaaaaa tcaagtctct ctcacttgtc tggtaaaggg attctatcca     1200

agtgatattg ctgtcgaatg ggagagcaac gggcaaccgg agaacaatta caaaacgaca     1260

cctcccgtcc ttgatagtga cgggtccttc ttcctgtatt caaagcttac agtcgacaaa     1320

agtcgctggc agcaggggaa tgtctttagc tgtagtgtca tgcacgaagc tttgcataac     1380

cactacacgc aaaagtctct tagcctgtcc ccaggaaagt tctgggtact ggtggttgtg     1440

ggcggcgttc tggcttgcta ttcactcctc gttacggttg catttataat attttgggta     1500

cgctcaaagc gctcaagatt gctccattct gattacatga acatgacccc tcgccggccg     1560

ggtcccactc gcaaacacta ccaaccttat gcccctccgc gggatttcgc ggcctacagg     1620

tcacgagtca agttttctcg aagtgccgac gctcctgcct atcagcaagg ccagaatcaa     1680

ctgtataacg agcttaactt gggccggagg gaggagtacg atgtgctgga taagcgcagg     1740

ggacgggacc cggaaatggg cggaaagccc aggcggaaaa acccacagga aggactctac     1800

aatgaactcc aaaaggacaa aatggcagag gcctattcag agattgggat gaagggagaa     1860

aggaggcgag gaaaagggca cgacggcttg tatcagggct tgtctacggc cactaaagac     1920

acctatgatg ccctgcatat gcaggcactt ccccccaga                            1959


