                         SEQUENCE LISTING

<110>  Schmidt, Monica
 
<120>  ELIMINATION OF AFLATOXIN VIA AFLATOXIN-DEGRADING ENZYME 
       EXPRESSION IN CROPS

<130>  UNIA 21.05

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  695
<212>  PRT
<213>  Armillaria tabescens

<400>  1

Met Ala Thr Thr Thr Val His Arg Glu Arg Phe Leu Ala Asp Lys Ser 
1               5                   10                  15      


Ala Pro Leu Cys Gly Met Asp Ile Arg Lys Ser Phe Asp Gln Leu Ser 
            20                  25                  30          


Ser Lys Glu Lys Leu Tyr Thr His Tyr Val Thr Glu Ala Ser Trp Ala 
        35                  40                  45              


Gly Ala Arg Ile Ile Gln Ala Gln Trp Thr Pro Gln Ala Thr Asp Leu 
    50                  55                  60                  


Tyr Asp Leu Leu Ile Leu Thr Phe Ser Val Asn Gly Lys Leu Ala Asp 
65                  70                  75                  80  


Leu Asn Ala Leu Lys Thr Ser Ser Gly Leu Ser Glu Asp Asp Trp Glu 
                85                  90                  95      


Ala Leu Ile Gln Tyr Thr Val Gln Val Leu Ser Asn Leu Val Asn Tyr 
            100                 105                 110         


Lys Thr Phe Gly Phe Thr Lys Ile Ile Pro Arg Val Asp Ala Glu Lys 
        115                 120                 125             


Phe Glu Ser Val Val Lys Ala Ser Ser Asn Ala Asp Gln Gly Ser Ala 
    130                 135                 140                 


Leu Phe Thr Lys Leu Lys Gln His Ile Tyr Ala Leu Ser Pro Glu Ser 
145                 150                 155                 160 


Ala Leu Phe Ile Gly Lys Arg Lys Asp Gly His Val Ser Asn Tyr Tyr 
                165                 170                 175     


Leu Gly Glu Pro Val Gly Asp Ala Glu Val Asp Ala Ile Gln Asn Val 
            180                 185                 190         


Ala Glu Lys Leu Gly Val Asp Ile Leu Asn Thr Arg Val Lys Lys Asn 
        195                 200                 205             


Gly Ala Gly Asp Tyr Thr Leu Leu Val Ala Ser Ala Lys Thr Ser Pro 
    210                 215                 220                 


Pro Ser Val His Asp Phe Gln Ile Asp Ser Thr Pro Ala Lys Leu Thr 
225                 230                 235                 240 


Ile Glu Tyr Gly Asp Tyr Ala Ser Ser Leu Thr Lys Val Val Ala Ala 
                245                 250                 255     


Leu Gln Glu Ala Lys Gln Tyr Thr Ala Asn Asp His Gln Ser Ala Met 
            260                 265                 270         


Ile Glu Gly Tyr Val Lys Ser Phe Asn Ser Gly Ser Ile Pro Glu His 
        275                 280                 285             


Lys Ala Ala Ser Thr Glu Trp Val Lys Asp Ile Gly Pro Val Val Glu 
    290                 295                 300                 


Ser Tyr Ile Gly Phe Val Glu Thr Tyr Val Asp Pro Tyr Gly Gly Arg 
305                 310                 315                 320 


Ala Glu Trp Glu Gly Phe Thr Ala Ile Val Asp Lys Gln Leu Ser Ala 
                325                 330                 335     


Lys Tyr Glu Ala Leu Val Asn Gly Ala Pro Lys Leu Ile Lys Ser Leu 
            340                 345                 350         


Pro Trp Gly Thr Asp Phe Glu Val Asp Val Phe Arg Lys Pro Asp Phe 
        355                 360                 365             


Thr Ala Leu Glu Val Val Ser Phe Ala Thr Gly Gly Ile Pro Ala Gly 
    370                 375                 380                 


Ile Asn Ile Pro Asn Tyr Tyr Glu Val Arg Glu Ser Thr Gly Phe Lys 
385                 390                 395                 400 


Asn Val Ser Leu Ala Asn Ile Leu Ala Ala Lys Val Pro Asn Glu Glu 
                405                 410                 415     


Leu Thr Phe Ile His Pro Asp Asp Val Glu Leu Tyr Asn Ala Trp Asp 
            420                 425                 430         


Ser Arg Ala Phe Glu Leu Gln Val Ala Asn His Glu Leu Leu Gly His 
        435                 440                 445             


Gly Ser Gly Lys Leu Phe Gln Glu Gly Ala Asp Gly Lys Leu Asn Phe 
    450                 455                 460                 


Asp Pro Glu Lys Val Ile Asn Pro Leu Thr Gly Lys Pro Ile Thr Ser 
465                 470                 475                 480 


Trp Tyr Lys Pro Gly Gln Thr Pro Asp Ser Val Leu Gly Glu Val Ser 
                485                 490                 495     


Ser Ser Met Glu Glu Cys Arg Ala Glu Thr Val Ala Leu Tyr Leu Val 
            500                 505                 510         


Ser Asn Leu Asp Ile Leu Lys Ile Phe Asn Tyr Val Asp Lys Gln Asp 
        515                 520                 525             


Ile Glu Asp Ile Gln Tyr Ile Thr Phe Leu Leu Met Ala Arg Ala Gly 
    530                 535                 540                 


Leu Arg Ala Leu Glu Phe Tyr Asp Pro Ala Thr Lys Lys His Gly Gln 
545                 550                 555                 560 


Ala His Met Gln Ala Arg Met Gly Ile Thr Gln Tyr Leu Ile Gln Ala 
                565                 570                 575     


Gly Ile Ala Arg Leu Glu Leu Ile Gln Asp Ala Asn Gly Glu Leu Glu 
            580                 585                 590         


Asn Leu Tyr Val Arg Val Asp Arg Glu Lys Val Leu Ser Lys Gly Lys 
        595                 600                 605             


Glu Val Val Gly Gln Leu Leu Ile Glu Leu Gln Val Arg Lys Ser Thr 
    610                 615                 620                 


Ala Asp Gly Thr Gly Ser Arg Asp Phe Tyr Thr Thr Leu Thr Glu Pro 
625                 630                 635                 640 


Ile Ser Gly Trp Glu Gly Lys Ile Arg Asp Ile Val Leu Lys Lys Lys 
                645                 650                 655     


Leu Pro Arg Lys Ile Phe Val Gln Pro Asn Thr Phe Val Val Asn Gly 
            660                 665                 670         


Glu Val Gln Leu Lys Glu Tyr Pro Leu Thr Ala Ala Gly Val Ile Glu 
        675                 680                 685             


Ser Phe Ile Glu Arg Arg Leu 
    690                 695 


<210>  2
<211>  722
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  protein encoding section of expression cassette

<400>  2

Met Lys Thr Asn Leu Phe Leu Phe Leu Ile Phe Ser Leu Leu Leu Ser 
1               5                   10                  15      


Leu Ser Ser Ala Glu Phe Met Ala Thr Thr Thr Val His Arg Glu Arg 
            20                  25                  30          


Phe Leu Ala Asp Lys Ser Ala Pro Leu Cys Gly Met Asp Ile Arg Lys 
        35                  40                  45              


Ser Phe Asp Gln Leu Ser Ser Lys Glu Lys Leu Tyr Thr His Tyr Val 
    50                  55                  60                  


Thr Glu Ala Ser Trp Ala Gly Ala Arg Ile Ile Gln Ala Gln Trp Thr 
65                  70                  75                  80  


Pro Gln Ala Thr Asp Leu Tyr Asp Leu Leu Ile Leu Thr Phe Ser Val 
                85                  90                  95      


Asn Gly Lys Leu Ala Asp Leu Asn Ala Leu Lys Thr Ser Ser Gly Leu 
            100                 105                 110         


Ser Glu Asp Asp Trp Glu Ala Leu Ile Gln Tyr Thr Val Gln Val Leu 
        115                 120                 125             


Ser Asn Leu Val Asn Tyr Lys Thr Phe Gly Phe Thr Lys Ile Ile Pro 
    130                 135                 140                 


Arg Val Asp Ala Glu Lys Phe Glu Ser Val Val Lys Ala Ser Ser Asn 
145                 150                 155                 160 


Ala Asp Gln Gly Ser Ala Leu Phe Thr Lys Leu Lys Gln His Ile Tyr 
                165                 170                 175     


Ala Leu Ser Pro Glu Ser Ala Leu Phe Ile Gly Lys Arg Lys Asp Gly 
            180                 185                 190         


His Val Ser Asn Tyr Tyr Leu Gly Glu Pro Val Gly Asp Ala Glu Val 
        195                 200                 205             


Asp Ala Ile Gln Asn Val Ala Glu Lys Leu Gly Val Asp Ile Leu Asn 
    210                 215                 220                 


Thr Arg Val Lys Lys Asn Gly Ala Gly Asp Tyr Thr Leu Leu Val Ala 
225                 230                 235                 240 


Ser Ala Lys Thr Ser Pro Pro Ser Val His Asp Phe Gln Ile Asp Ser 
                245                 250                 255     


Thr Pro Ala Lys Leu Thr Ile Glu Tyr Gly Asp Tyr Ala Ser Ser Leu 
            260                 265                 270         


Thr Lys Val Val Ala Ala Leu Gln Glu Ala Lys Gln Tyr Thr Ala Asn 
        275                 280                 285             


Asp His Gln Ser Ala Met Ile Glu Gly Tyr Val Lys Ser Phe Asn Ser 
    290                 295                 300                 


Gly Ser Ile Pro Glu His Lys Ala Ala Ser Thr Glu Trp Val Lys Asp 
305                 310                 315                 320 


Ile Gly Pro Val Val Glu Ser Tyr Ile Gly Phe Val Glu Thr Tyr Val 
                325                 330                 335     


Asp Pro Tyr Gly Gly Arg Ala Glu Trp Glu Gly Phe Thr Ala Ile Val 
            340                 345                 350         


Asp Lys Gln Leu Ser Ala Lys Tyr Glu Ala Leu Val Asn Gly Ala Pro 
        355                 360                 365             


Lys Leu Ile Lys Ser Leu Pro Trp Gly Thr Asp Phe Glu Val Asp Val 
    370                 375                 380                 


Phe Arg Lys Pro Asp Phe Thr Ala Leu Glu Val Val Ser Phe Ala Thr 
385                 390                 395                 400 


Gly Gly Ile Pro Ala Gly Ile Asn Ile Pro Asn Tyr Tyr Glu Val Arg 
                405                 410                 415     


Glu Ser Thr Gly Phe Lys Asn Val Ser Leu Ala Asn Ile Leu Ala Ala 
            420                 425                 430         


Lys Val Pro Asn Glu Glu Leu Thr Phe Ile His Pro Asp Asp Val Glu 
        435                 440                 445             


Leu Tyr Asn Ala Trp Asp Ser Arg Ala Phe Glu Leu Gln Val Ala Asn 
    450                 455                 460                 


His Glu Leu Leu Gly His Gly Ser Gly Lys Leu Phe Gln Glu Gly Ala 
465                 470                 475                 480 


Asp Gly Lys Leu Asn Phe Asp Pro Glu Lys Val Ile Asn Pro Leu Thr 
                485                 490                 495     


Gly Lys Pro Ile Thr Ser Trp Tyr Lys Pro Gly Gln Thr Pro Asp Ser 
            500                 505                 510         


Val Leu Gly Glu Val Ser Ser Ser Met Glu Glu Cys Arg Ala Glu Thr 
        515                 520                 525             


Val Ala Leu Tyr Leu Val Ser Asn Leu Asp Ile Leu Lys Ile Phe Asn 
    530                 535                 540                 


Tyr Val Asp Lys Gln Asp Ile Glu Asp Ile Gln Tyr Ile Thr Phe Leu 
545                 550                 555                 560 


Leu Met Ala Arg Ala Gly Leu Arg Ala Leu Glu Phe Tyr Asp Pro Ala 
                565                 570                 575     


Thr Lys Lys His Gly Gln Ala His Met Gln Ala Arg Met Gly Ile Thr 
            580                 585                 590         


Gln Tyr Leu Ile Gln Ala Gly Ile Ala Arg Leu Glu Leu Ile Gln Asp 
        595                 600                 605             


Ala Asn Gly Glu Leu Glu Asn Leu Tyr Val Arg Val Asp Arg Glu Lys 
    610                 615                 620                 


Val Leu Ser Lys Gly Lys Glu Val Val Gly Gln Leu Leu Ile Glu Leu 
625                 630                 635                 640 


Gln Val Arg Lys Ser Thr Ala Asp Gly Thr Gly Ser Arg Asp Phe Tyr 
                645                 650                 655     


Thr Thr Leu Thr Glu Pro Ile Ser Gly Trp Glu Gly Lys Ile Arg Asp 
            660                 665                 670         


Ile Val Leu Lys Lys Lys Leu Pro Arg Lys Ile Phe Val Gln Pro Asn 
        675                 680                 685             


Thr Phe Val Val Asn Gly Glu Val Gln Leu Lys Glu Tyr Pro Leu Thr 
    690                 695                 700                 


Ala Ala Gly Val Ile Glu Ser Phe Ile Glu Arg Arg Leu Lys His Asp 
705                 710                 715                 720 


Glu Leu 
        


<210>  3
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ER retention sequence

<400>  3

Lys His Asp Glu Leu 
1               5   


<210>  4
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ER retention sequence

<400>  4

Lys Asp Glu Leu 
1               


<210>  5
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer specific to the inserted aflatoxin-degrading 
       enzyme sequence

<400>  5
gttggcagat cttaacgctc t                                                 21


<210>  6
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer specific to the inserted aflatoxin-degrading 
       enzyme sequence

<400>  6
cttcccattc agccctacct c                                                 21


