                         SEQUENCE LISTING

<110>  Oxford University Innovation Limited
 
<120>  Polypeptides that interact with peptide tags at loops or termini 
       and uses thereof

<130>  20.153906/01

<150>  GB2104999.4
<151>  2021-04-08

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  104
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DogCatcher

<400>  1

Lys Leu Gly Glu Ile Glu Phe Ile Lys Val Asp Lys Thr Asp Lys Lys 
1               5                   10                  15      


Pro Leu Arg Gly Ala Val Phe Ser Leu Gln Lys Gln His Pro Asp Tyr 
            20                  25                  30          


Pro Asp Ile Tyr Gly Ala Ile Asp Gln Asn Gly Thr Tyr Gln Asp Val 
        35                  40                  45              


Arg Thr Gly Glu Asp Gly Lys Leu Thr Phe Thr Asn Leu Ser Asp Gly 
    50                  55                  60                  


Lys Tyr Arg Leu Ile Glu Asn Ser Glu Pro Pro Gly Tyr Lys Pro Val 
65                  70                  75                  80  


Gln Asn Lys Pro Ile Val Ser Phe Arg Ile Val Asp Gly Glu Val Arg 
                85                  90                  95      


Asp Val Thr Ser Ile Val Pro Gln 
            100                 


<210>  2
<211>  95
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DogCatcher Truncation

<400>  2

Ile Glu Phe Ile Lys Val Asp Lys Thr Asp Lys Lys Pro Leu Arg Gly 
1               5                   10                  15      


Ala Val Phe Ser Leu Gln Lys Gln His Pro Asp Tyr Pro Asp Ile Tyr 
            20                  25                  30          


Gly Ala Ile Asp Gln Asn Gly Thr Tyr Gln Asp Val Arg Thr Gly Glu 
        35                  40                  45              


Asp Gly Lys Leu Thr Phe Thr Asn Leu Ser Asp Gly Lys Tyr Arg Leu 
    50                  55                  60                  


Ile Glu Asn Ser Glu Pro Pro Gly Tyr Lys Pro Val Gln Asn Lys Pro 
65                  70                  75                  80  


Ile Val Ser Phe Arg Ile Val Asp Gly Glu Val Arg Asp Val Thr 
                85                  90                  95  


<210>  3
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DogTag

<400>  3

Asp Ile Pro Ala Thr Tyr Glu Phe Thr Asp Gly Lys His Tyr Ile Thr 
1               5                   10                  15      


Asn Glu Pro Ile Pro Pro Lys 
            20              


<210>  4
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RrgATag

<400>  4

Asp Ile Pro Ala Thr Tyr Glu Phe Thr Asn Asp Lys His Tyr Ile Thr 
1               5                   10                  15      


Asn Glu Pro 
            


<210>  5
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RrgATag2

<400>  5

Asp Ile Pro Ala Thr Tyr Glu Phe Thr Asn Gly Lys His Tyr Ile Thr 
1               5                   10                  15      


Asn Glu Pro Ile Pro Pro Lys 
            20              


<210>  6
<211>  104
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RrgACatcher

<400>  6

Lys Leu Gly Asp Ile Glu Phe Ile Lys Val Asn Lys Asn Asp Lys Lys 
1               5                   10                  15      


Pro Leu Arg Gly Ala Val Phe Ser Leu Gln Lys Gln His Pro Asp Tyr 
            20                  25                  30          


Pro Asp Ile Tyr Gly Ala Ile Asp Gln Asn Gly Thr Tyr Gln Asn Val 
        35                  40                  45              


Arg Thr Gly Glu Asp Gly Lys Leu Thr Phe Lys Asn Leu Ser Asp Gly 
    50                  55                  60                  


Lys Tyr Arg Leu Phe Glu Asn Ser Glu Pro Ala Gly Tyr Lys Pro Val 
65                  70                  75                  80  


Gln Asn Lys Pro Ile Val Ala Phe Gln Ile Val Asn Gly Glu Val Arg 
                85                  90                  95      


Asp Val Thr Ser Ile Val Pro Gln 
            100                 


<210>  7
<211>  312
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DogCatcher DNA

<400>  7
aaactgggcg agattgaatt tattaaagtg gacaaaaccg ataaaaagcc gctgcgtggt       60

gccgtgttta gcctgcagaa acagcatccc gactatcccg atatctatgg cgcgattgat      120

cagaatggga cctatcaaga tgtgcgtacc ggcgaagatg gtaaactgac ctttacgaat      180

ctgagcgatg gcaaatatcg cctgattgaa aatagcgaac ccccgggcta taaaccggtg      240

cagaataagc cgattgtgag ctttcgtatt gtggatggcg aagtgcgtga tgtgaccagt      300

attgtgccgc ag                                                          312


<210>  8
<211>  104
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RrgACatcherB

<400>  8

Lys Leu Gly Glu Ile Glu Phe Ile Lys Val Asp Lys Thr Asp Lys Lys 
1               5                   10                  15      


Pro Leu Arg Gly Ala Val Phe Ser Leu Gln Lys Gln His Pro Asp Tyr 
            20                  25                  30          


Pro Asp Ile Tyr Gly Ala Ile Asp Gln Asn Gly Thr Tyr Gln Asp Val 
        35                  40                  45              


Arg Thr Gly Glu Asp Gly Lys Leu Thr Phe Thr Asn Leu Ser Asp Gly 
    50                  55                  60                  


Lys Tyr Arg Leu Phe Glu Asn Ser Glu Pro Pro Gly Tyr Lys Pro Val 
65                  70                  75                  80  


Gln Asn Lys Pro Ile Val Ala Phe Gln Ile Val Asp Gly Glu Val Arg 
                85                  90                  95      


Asp Val Thr Ser Ile Val Pro Gln 
            100                 


<210>  9
<211>  104
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RrgACatcher with reactivity modifications

<400>  9

Lys Leu Gly Asp Ile Glu Phe Ile Lys Val Asn Lys Asn Asp Lys Lys 
1               5                   10                  15      


Pro Leu Arg Gly Ala Val Phe Ser Leu Gln Lys Gln His Pro Asp Tyr 
            20                  25                  30          


Pro Asp Ile Tyr Gly Ala Ile Asp Gln Asn Gly Thr Tyr Gln Asn Val 
        35                  40                  45              


Arg Thr Gly Glu Asp Gly Lys Leu Thr Phe Lys Asn Leu Ser Asp Gly 
    50                  55                  60                  


Lys Tyr Arg Leu Ile Glu Asn Ser Glu Pro Ala Gly Tyr Lys Pro Val 
65                  70                  75                  80  


Gln Asn Lys Pro Ile Val Ser Phe Arg Ile Val Asn Gly Glu Val Arg 
                85                  90                  95      


Asp Val Thr Ser Ile Val Pro Gln 
            100                 


<210>  10
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Spacer-SpyTag003-Spacer

<400>  10

Gly Gly Gly Gly Ser Arg Gly Val Pro His Ile Val Met Val Asp Ala 
1               5                   10                  15      


Tyr Lys Arg Tyr Lys Gly Gly Gly Gly Ser 
            20                  25      


<210>  11
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Spacer-DogTag-Spacer

<400>  11

Gly Gly Gly Gly Ser Asp Ile Pro Ala Thr Tyr Glu Phe Thr Asp Gly 
1               5                   10                  15      


Lys His Tyr Ile Thr Asn Glu Pro Ile Pro Pro Lys Gly Gly Gly Gly 
            20                  25                  30          


Ser 
    


<210>  12
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  12
ggatccagtg gtagcgaaaa cctctac                                           27


<210>  13
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  13
catggcgccc tgatctcgag g                                                 21


<210>  14
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  14
gacctcgaga tcagggcgcc atg                                               23


<210>  15
<211>  29
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  15
gaagtagagg ttttcgctac cactggatc                                         29


<210>  16
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  16

Gly Thr Ser Gly Ser Gly Gly Ser Gly 
1               5                   


<210>  17
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  R2Tag

<400>  17

Asp Ile Pro Ala Gly Tyr Glu Phe Thr Asn Asp Lys His Tyr Ile Thr 
1               5                   10                  15      


Asn Glu Pro Ile Pro Pro Lys 
            20              


<210>  18
<211>  104
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Affinity purification polypeptide


<220>
<221>  MISC_FEATURE
<222>  (70)..(70)
<223>  Xaa is not glutamic acid or aspartic acid

<400>  18

Lys Leu Gly Glu Ile Glu Phe Ile Lys Val Asp Lys Thr Asp Lys Lys 
1               5                   10                  15      


Pro Leu Arg Gly Ala Val Phe Ser Leu Gln Lys Gln His Pro Asp Tyr 
            20                  25                  30          


Pro Asp Ile Tyr Gly Ala Ile Asp Gln Asn Gly Thr Tyr Gln Asp Val 
        35                  40                  45              


Arg Thr Gly Glu Asp Gly Lys Leu Thr Phe Thr Asn Leu Ser Asp Gly 
    50                  55                  60                  


Lys Tyr Arg Leu Ile Xaa Asn Ser Glu Pro Pro Gly Tyr Lys Pro Val 
65                  70                  75                  80  


Gln Asn Lys Pro Ile Val Ser Phe Arg Ile Val Asp Gly Glu Val Arg 
                85                  90                  95      


Asp Val Thr Ser Ile Val Pro Gln 
            100                 


<210>  19
<211>  95
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Truncated Affinity purification polypeptide


<220>
<221>  MISC_FEATURE
<222>  (66)..(66)
<223>  Xaa is not glutamic acid or aspartic acid

<400>  19

Ile Glu Phe Ile Lys Val Asp Lys Thr Asp Lys Lys Pro Leu Arg Gly 
1               5                   10                  15      


Ala Val Phe Ser Leu Gln Lys Gln His Pro Asp Tyr Pro Asp Ile Tyr 
            20                  25                  30          


Gly Ala Ile Asp Gln Asn Gly Thr Tyr Gln Asp Val Arg Thr Gly Glu 
        35                  40                  45              


Asp Gly Lys Leu Thr Phe Thr Asn Leu Ser Asp Gly Lys Tyr Arg Leu 
    50                  55                  60                  


Ile Xaa Asn Ser Glu Pro Pro Gly Tyr Lys Pro Val Gln Asn Lys Pro 
65                  70                  75                  80  


Ile Val Ser Phe Arg Ile Val Asp Gly Glu Val Arg Asp Val Thr 
                85                  90                  95  


<210>  20
<211>  131
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Affinity purification polypeptide extension


<220>
<221>  MISC_FEATURE
<222>  (97)..(97)
<223>  Xaa is not glutamic acid or aspartic acid

<400>  20

Met Ser Tyr Tyr His His His His His His Asp Tyr Asp Ile Pro Thr 
1               5                   10                  15      


Thr Glu Asn Leu Tyr Phe Gln Gly Gly Cys Gly Lys Leu Gly Glu Ile 
            20                  25                  30          


Glu Phe Ile Lys Val Asp Lys Thr Asp Lys Lys Pro Leu Arg Gly Ala 
        35                  40                  45              


Val Phe Ser Leu Gln Lys Gln His Pro Asp Tyr Pro Asp Ile Tyr Gly 
    50                  55                  60                  


Ala Ile Asp Gln Asn Gly Thr Tyr Gln Asp Val Arg Thr Gly Glu Asp 
65                  70                  75                  80  


Gly Lys Leu Thr Phe Thr Asn Leu Ser Asp Gly Lys Tyr Arg Leu Ile 
                85                  90                  95      


Xaa Asn Ser Glu Pro Pro Gly Tyr Lys Pro Val Gln Asn Lys Pro Ile 
            100                 105                 110         


Val Ser Phe Arg Ile Val Asp Gly Glu Val Arg Asp Val Thr Ser Ile 
        115                 120                 125             


Val Pro Gln 
    130     


<210>  21
<211>  104
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Affinity purification polypeptide D31C


<220>
<221>  MISC_FEATURE
<222>  (70)..(70)
<223>  Xaa is not glutamic acid or aspartic acid

<400>  21

Lys Leu Gly Glu Ile Glu Phe Ile Lys Val Asp Lys Thr Asp Lys Lys 
1               5                   10                  15      


Pro Leu Arg Gly Ala Val Phe Ser Leu Gln Lys Gln His Pro Cys Tyr 
            20                  25                  30          


Pro Asp Ile Tyr Gly Ala Ile Asp Gln Asn Gly Thr Tyr Gln Asp Val 
        35                  40                  45              


Arg Thr Gly Glu Asp Gly Lys Leu Thr Phe Thr Asn Leu Ser Asp Gly 
    50                  55                  60                  


Lys Tyr Arg Leu Ile Xaa Asn Ser Glu Pro Pro Gly Tyr Lys Pro Val 
65                  70                  75                  80  


Gln Asn Lys Pro Ile Val Ser Phe Arg Ile Val Asp Gly Glu Val Arg 
                85                  90                  95      


Asp Val Thr Ser Ile Val Pro Gln 
            100                 


<210>  22
<211>  104
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Affinity purification polypeptide Q41C


<220>
<221>  MISC_FEATURE
<222>  (70)..(70)
<223>  Xaa is not glutamic acid or aspartic acid

<400>  22

Lys Leu Gly Glu Ile Glu Phe Ile Lys Val Asp Lys Thr Asp Lys Lys 
1               5                   10                  15      


Pro Leu Arg Gly Ala Val Phe Ser Leu Gln Lys Gln His Pro Asp Tyr 
            20                  25                  30          


Pro Asp Ile Tyr Gly Ala Ile Asp Cys Asn Gly Thr Tyr Gln Asp Val 
        35                  40                  45              


Arg Thr Gly Glu Asp Gly Lys Leu Thr Phe Thr Asn Leu Ser Asp Gly 
    50                  55                  60                  


Lys Tyr Arg Leu Ile Xaa Asn Ser Glu Pro Pro Gly Tyr Lys Pro Val 
65                  70                  75                  80  


Gln Asn Lys Pro Ile Val Ser Phe Arg Ile Val Asp Gly Glu Val Arg 
                85                  90                  95      


Asp Val Thr Ser Ile Val Pro Gln 
            100                 


<210>  23
<211>  95
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Truncated Affinity purification polypeptide D27C


<220>
<221>  MISC_FEATURE
<222>  (66)..(66)
<223>  Xaa is not glutamic acid or aspartic acid

<400>  23

Ile Glu Phe Ile Lys Val Asp Lys Thr Asp Lys Lys Pro Leu Arg Gly 
1               5                   10                  15      


Ala Val Phe Ser Leu Gln Lys Gln His Pro Cys Tyr Pro Asp Ile Tyr 
            20                  25                  30          


Gly Ala Ile Asp Gln Asn Gly Thr Tyr Gln Asp Val Arg Thr Gly Glu 
        35                  40                  45              


Asp Gly Lys Leu Thr Phe Thr Asn Leu Ser Asp Gly Lys Tyr Arg Leu 
    50                  55                  60                  


Ile Xaa Asn Ser Glu Pro Pro Gly Tyr Lys Pro Val Gln Asn Lys Pro 
65                  70                  75                  80  


Ile Val Ser Phe Arg Ile Val Asp Gly Glu Val Arg Asp Val Thr 
                85                  90                  95  


<210>  24
<211>  95
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Truncated Affinity purification polypeptide Q37C


<220>
<221>  MISC_FEATURE
<222>  (66)..(66)
<223>  Xaa is not glutamic acid or aspartic acid

<400>  24

Ile Glu Phe Ile Lys Val Asp Lys Thr Asp Lys Lys Pro Leu Arg Gly 
1               5                   10                  15      


Ala Val Phe Ser Leu Gln Lys Gln His Pro Asp Tyr Pro Asp Ile Tyr 
            20                  25                  30          


Gly Ala Ile Asp Cys Asn Gly Thr Tyr Gln Asp Val Arg Thr Gly Glu 
        35                  40                  45              


Asp Gly Lys Leu Thr Phe Thr Asn Leu Ser Asp Gly Lys Tyr Arg Leu 
    50                  55                  60                  


Ile Xaa Asn Ser Glu Pro Pro Gly Tyr Lys Pro Val Gln Asn Lys Pro 
65                  70                  75                  80  


Ile Val Ser Phe Arg Ile Val Asp Gly Glu Val Arg Asp Val Thr 
                85                  90                  95  


