               SEQUENCE LISTING

<110> Consejo Superior de Investigaciones Científicas (CSIC)
      Universidad Autónoma de Madrid (UAM)

<120> A new non-aggregative splicing isoform of human Tau is decreased in Alzheimer’s disease

<130> 907 094

<140> EP21382283.6
<141> 2021-04-05

<150> EP21 382 283.6
<151> 2021-04-05

<160> 63

<170> BiSSAP 1.3.6

<210> 1
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> tau-isoform C-terminus domain amino acid sequence

<400> 1
Trp Val Gly Cys Cys Pro Trp 
1               5           

<210> 2
<211> 18
<212> PRT
<213> Artificial Sequence


<220> 
<223> tau-isoform C-terminus domain amino acid

<400> 2
Lys Lys Val Lys Gly Val Gly Trp Val Gly Cys Cys Pro Trp Val Tyr 
1               5                   10                  15      
Gly His 
        

<210> 3
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> peptide inhibitor

<400> 3
Gly Val Gly Trp Val Gly 
1               5       

<210> 4
<211> 8
<212> PRT
<213> Artificial Sequence


<220> 
<223> peptide inhibitor

<400> 4
Gly Trp Val Gly Cys Cys Pro Trp 
1               5               

<210> 5
<211> 9
<212> PRT
<213> Artificial Sequence


<220> 
<223> peptide inhibitor

<400> 5
Val Gly Trp Val Gly Cys Cys Pro Trp 
1               5                   

<210> 6
<211> 10
<212> PRT
<213> Artificial Sequence


<220> 
<223> peptide inhibitor

<400> 6
Gly Val Gly Trp Val Gly Cys Cys Pro Trp 
1               5                   10  

<210> 7
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> peptide inhibitor

<400> 7
Lys Gly Val Gly Trp Val Gly Cys Cys Pro Trp 
1               5                   10      

<210> 8
<211> 12
<212> PRT
<213> Artificial Sequence


<220> 
<223> peptide inhibitor

<400> 8
Val Lys Gly Val Gly Trp Val Gly Cys Cys Pro Trp 
1               5                   10          

<210> 9
<211> 8
<212> PRT
<213> Artificial Sequence


<220> 
<223> peptide inhibitor

<400> 9
Trp Val Gly Cys Cys Pro Trp Val 
1               5               

<210> 10
<211> 9
<212> PRT
<213> Artificial Sequence


<220> 
<223> peptide inhibitor

<400> 10
Gly Trp Val Gly Cys Cys Pro Trp Val 
1               5                   

<210> 11
<211> 9
<212> PRT
<213> Artificial Sequence


<220> 
<223> peptide inhibitor

<400> 11
Trp Val Gly Cys Cys Pro Trp Val Tyr 
1               5                   

<210> 12
<211> 10
<212> PRT
<213> Artificial Sequence


<220> 
<223> peptide inhibitor

<400> 12
Trp Val Gly Cys Cys Pro Trp Val Tyr Gly 
1               5                   10  

<210> 13
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> peptide inhibitor

<400> 13
Trp Val Gly Cys Cys Pro Trp Val Tyr Gly His 
1               5                   10      

<210> 14
<211> 297
<212> PRT
<213> Artificial Sequence


<220> 
<223> peptide inhibitor

<400> 14
Met Ala Glu Pro Arg Gln Glu Phe Glu Val Met Glu Asp His Ala Gly 
1               5                   10                  15      
Thr Tyr Gly Leu Gly Asp Arg Lys Asp Gln Gly Gly Tyr Thr Met His 
            20                  25                  30          
Gln Asp Gln Glu Gly Asp Thr Asp Ala Gly Leu Lys Ala Glu Glu Ala 
        35                  40                  45              
Gly Ile Gly Asp Thr Pro Ser Leu Glu Asp Glu Ala Ala Gly His Val 
    50                  55                  60                  
Thr Gln Ala Arg Met Val Ser Lys Ser Lys Asp Gly Thr Gly Ser Asp 
65                  70                  75                  80  
Asp Lys Lys Ala Lys Gly Ala Asp Gly Lys Thr Lys Ile Ala Thr Pro 
                85                  90                  95      
Arg Gly Ala Ala Pro Pro Gly Gln Lys Gly Gln Ala Asn Ala Thr Arg 
            100                 105                 110         
Ile Pro Ala Lys Thr Pro Pro Ala Pro Lys Thr Pro Pro Ser Ser Gly 
        115                 120                 125             
Glu Pro Pro Lys Ser Gly Asp Arg Ser Gly Tyr Ser Ser Pro Gly Ser 
    130                 135                 140                 
Pro Gly Thr Pro Gly Ser Arg Ser Arg Thr Pro Ser Leu Pro Thr Pro 
145                 150                 155                 160 
Pro Thr Arg Glu Pro Lys Lys Val Ala Val Val Arg Thr Pro Pro Lys 
                165                 170                 175     
Ser Pro Ser Ser Ala Lys Ser Arg Leu Gln Thr Ala Pro Val Pro Met 
            180                 185                 190         
Pro Asp Leu Lys Asn Val Lys Ser Lys Ile Gly Ser Thr Glu Asn Leu 
        195                 200                 205             
Lys His Gln Pro Gly Gly Gly Lys Val Gln Ile Val Tyr Lys Pro Val 
    210                 215                 220                 
Asp Leu Ser Lys Val Thr Ser Lys Cys Gly Ser Leu Gly Asn Ile His 
225                 230                 235                 240 
His Lys Pro Gly Gly Gly Gln Val Glu Val Lys Ser Glu Lys Leu Asp 
                245                 250                 255     
Phe Lys Asp Arg Val Gln Ser Lys Ile Gly Ser Leu Asp Asn Ile Thr 
            260                 265                 270         
His Val Pro Gly Gly Gly Asn Lys Lys Val Lys Gly Val Gly Trp Val 
        275                 280                 285             
Gly Cys Cys Pro Trp Val Tyr Gly His 
    290                 295         

<210> 15
<211> 386
<212> PRT
<213> Artificial Sequence


<220> 
<223> peptide inhibitor

<400> 15
Met Ala Glu Pro Arg Gln Glu Phe Glu Val Met Glu Asp His Ala Gly 
1               5                   10                  15      
Thr Tyr Gly Leu Gly Asp Arg Lys Asp Gln Gly Gly Tyr Thr Met His 
            20                  25                  30          
Gln Asp Gln Glu Gly Asp Thr Asp Ala Gly Leu Lys Glu Ser Pro Leu 
        35                  40                  45              
Gln Thr Pro Thr Glu Asp Gly Ser Glu Glu Pro Gly Ser Glu Thr Ser 
    50                  55                  60                  
Asp Ala Lys Ser Thr Pro Thr Ala Glu Asp Val Thr Ala Pro Leu Val 
65                  70                  75                  80  
Asp Glu Gly Ala Pro Gly Lys Gln Ala Ala Ala Gln Pro His Thr Glu 
                85                  90                  95      
Ile Pro Glu Gly Thr Thr Ala Glu Glu Ala Gly Ile Gly Asp Thr Pro 
            100                 105                 110         
Ser Leu Glu Asp Glu Ala Ala Gly His Val Thr Gln Ala Arg Met Val 
        115                 120                 125             
Ser Lys Ser Lys Asp Gly Thr Gly Ser Asp Asp Lys Lys Ala Lys Gly 
    130                 135                 140                 
Ala Asp Gly Lys Thr Lys Ile Ala Thr Pro Arg Gly Ala Ala Pro Pro 
145                 150                 155                 160 
Gly Gln Lys Gly Gln Ala Asn Ala Thr Arg Ile Pro Ala Lys Thr Pro 
                165                 170                 175     
Pro Ala Pro Lys Thr Pro Pro Ser Ser Gly Glu Pro Pro Lys Ser Gly 
            180                 185                 190         
Asp Arg Ser Gly Tyr Ser Ser Pro Gly Ser Pro Gly Thr Pro Gly Ser 
        195                 200                 205             
Arg Ser Arg Thr Pro Ser Leu Pro Thr Pro Pro Thr Arg Glu Pro Lys 
    210                 215                 220                 
Lys Val Ala Val Val Arg Thr Pro Pro Lys Ser Pro Ser Ser Ala Lys 
225                 230                 235                 240 
Ser Arg Leu Gln Thr Ala Pro Val Pro Met Pro Asp Leu Lys Asn Val 
                245                 250                 255     
Lys Ser Lys Ile Gly Ser Thr Glu Asn Leu Lys His Gln Pro Gly Gly 
            260                 265                 270         
Gly Lys Val Gln Ile Ile Asn Lys Lys Leu Asp Leu Ser Asn Val Gln 
        275                 280                 285             
Ser Lys Cys Gly Ser Lys Asp Asn Ile Lys His Val Pro Gly Gly Gly 
    290                 295                 300                 
Ser Val Gln Ile Val Tyr Lys Pro Val Asp Leu Ser Lys Val Thr Ser 
305                 310                 315                 320 
Lys Cys Gly Ser Leu Gly Asn Ile His His Lys Pro Gly Gly Gly Gln 
                325                 330                 335     
Val Glu Val Lys Ser Glu Lys Leu Asp Phe Lys Asp Arg Val Gln Ser 
            340                 345                 350         
Lys Ile Gly Ser Leu Asp Asn Ile Thr His Val Pro Gly Gly Gly Asn 
        355                 360                 365             
Lys Lys Val Lys Gly Val Gly Trp Val Gly Cys Cys Pro Trp Val Tyr 
    370                 375                 380                 
Gly His 
385     

<210> 16
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> C-terminus domain amino acid sequence

<400> 16
Gly Val Gly Trp Val Gly 
1               5       

<210> 17
<400> 17
000

<210> 18
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> TauA

<400> 18
accagttgac ctgagcaagg                                                20


<210> 19
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> TauB

<400> 19
ggtggccagg tggaagtaaa                                                20


<210> 20
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> TauC

<400> 20
gaccagcctt tgtccactca                                                20


<210> 21
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> TauD

<400> 21
agccagtcaa cacagaagcc                                                20


<210> 22
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> TauE

<400> 22
gacaccactg gcgacttgta                                                20


<210> 23
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> TauE

<400> 23
ccatcataaa ccaggaggtg gc                                             22


<210> 24
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> TauF

<400> 24
gcggcagtgt gcaaatagtc                                                20


<210> 25
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> GAPDH Fw

<400> 25
gagaaggctg gggctcattt                                                20


<210> 26
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> GAPDH Rv

<400> 26
agtgatggca tggactgtgg                                                20


<210> 27
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> TIR-T-fw

<400> 27
cataaaccag gaggtggcca g                                              21


<210> 28
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> TIR-T-rv

<400> 28
caccctaccc cctttacctt tt                                             22


<210> 29
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> MAPT-E11-E13-fw

<400> 29
gtcgaagatt gggtccctgg                                                20


<210> 30
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> MAPT-E11-E13-rv

<400> 30
gacaccactg gcgacttgta                                                20


<210> 31
<211> 36
<212> DNA
<213> Artificial Sequence


<220> 
<223> ET-T-PacI

<400> 31
gtttaattaa tcaatttcct ccgccaggga cgtggg                               36


<210> 32
<211> 32
<212> DNA
<213> Artificial Sequence


<220> 
<223> TIR-T-PacI

<400> 32
aattaattaa tgcccatata cccaagggca gc                                   32


<210> 33
<211> 33
<212> DNA
<213> Artificial Sequence


<220> 
<223> A22

<400> 33
caagatctca atttcctccg ccagggacgt ggg                                  33


<210> 34
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Tau-Nt

<400> 34
atggctgagc cccgccagga g                                              21


<210> 35
<211> 32
<212> DNA
<213> Artificial Sequence


<220> 
<223> TIR-T-BglII

<400> 35
gagatcttaa tgcccatata cccaagggca gc                                   32


<210> 36
<211> 36
<212> DNA
<213> Artificial Sequence


<220> 
<223> ET-T-PacI

<400> 36
gtttaattaa tcaatttcct ccgccaggga cgtggg                               36


<210> 37
<211> 32
<212> DNA
<213> Artificial Sequence


<220> 
<223> TIR-T-PacI

<400> 37
aattaattaa tgcccatata cccaagggca gc                                   32


<210> 38
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Tau-Nt

<400> 38
atggctgagc cccgccagga g                                              21


<210> 39
<211> 32
<212> DNA
<213> Artificial Sequence


<220> 
<223> TIR-T-BglII

<400> 39
gagatcttaa tgcccatata cccaagggca gc                                   32


<210> 40
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> TAU-PRK172 fw

<400> 40
gcggatccat atggctgagc cc                                             22


<210> 41
<211> 30
<212> DNA
<213> Artificial Sequence


<220> 
<223> TIR-T-pRKpWPI rv

<400> 41
gcgaattctt aatgcccata tacccaaggg                                     30


<210> 42
<211> 37
<212> DNA
<213> Artificial Sequence


<220> 
<223> ET-T-pRKpWPI rv

<400> 42
gttctgaatt cttaattaat caatttcctc cgccagg                              37


<210> 43
<211> 33
<212> DNA
<213> Artificial Sequence


<220> 
<223> A22

<400> 43
caagatctca atttcctccg ccagggacgt ggg                                  33


<210> 44
<211> 37
<212> DNA
<213> Artificial Sequence


<220> 
<223> A1

<400> 44
ggcgaattcg gatcctatgg ctgagccccg ccaggag                              37


<210> 45
<211> 18
<212> DNA
<213> Artificial Sequence


<220> 
<223> A4

<400> 45
gctgctcccc gcggtgtg                                                  18


<210> 46
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Tau-Ct

<400> 46
accctgcttg gccagggagg c                                              21


<210> 47
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Tau R1 Rv

<400> 47
ccgctgttgg agtgctctta                                                20


<210> 48
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Tau 447 Rv

<400> 48
cgttttacca tcagccccct                                                20


<210> 49
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> pSGTau-fw

<400> 49
ctcactatag ggcgaattca tg                                             22


<210> 50
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> pSGTau-rv

<400> 50
agcggaagag tctagagtcg                                                20


<210> 51
<211> 352
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tau with no inserts and 3 microtubule-binding domains (T30)

<400> 51
Met Ala Glu Pro Arg Gln Glu Phe Glu Val Met Glu Asp His Ala Gly 
1               5                   10                  15      
Thr Tyr Gly Leu Gly Asp Arg Lys Asp Gln Gly Gly Tyr Thr Met His 
            20                  25                  30          
Gln Asp Gln Glu Gly Asp Thr Asp Ala Gly Leu Lys Ala Glu Glu Ala 
        35                  40                  45              
Gly Ile Gly Asp Thr Pro Ser Leu Glu Asp Glu Ala Ala Gly His Val 
    50                  55                  60                  
Thr Gln Ala Arg Met Val Ser Lys Ser Lys Asp Gly Thr Gly Ser Asp 
65                  70                  75                  80  
Asp Lys Lys Ala Lys Gly Ala Asp Gly Lys Thr Lys Ile Ala Thr Pro 
                85                  90                  95      
Arg Gly Ala Ala Pro Pro Gly Gln Lys Gly Gln Ala Asn Ala Thr Arg 
            100                 105                 110         
Ile Pro Ala Lys Thr Pro Pro Ala Pro Lys Thr Pro Pro Ser Ser Gly 
        115                 120                 125             
Glu Pro Pro Lys Ser Gly Asp Arg Ser Gly Tyr Ser Ser Pro Gly Ser 
    130                 135                 140                 
Pro Gly Thr Pro Gly Ser Arg Ser Arg Thr Pro Ser Leu Pro Thr Pro 
145                 150                 155                 160 
Pro Thr Arg Glu Pro Lys Lys Val Ala Val Val Arg Thr Pro Pro Lys 
                165                 170                 175     
Ser Pro Ser Ser Ala Lys Ser Arg Leu Gln Thr Ala Pro Val Pro Met 
            180                 185                 190         
Pro Asp Leu Lys Asn Val Lys Ser Lys Ile Gly Ser Thr Glu Asn Leu 
        195                 200                 205             
Lys His Gln Pro Gly Gly Gly Lys Val Gln Ile Val Tyr Lys Pro Val 
    210                 215                 220                 
Asp Leu Ser Lys Val Thr Ser Lys Cys Gly Ser Leu Gly Asn Ile His 
225                 230                 235                 240 
His Lys Pro Gly Gly Gly Gln Val Glu Val Lys Ser Glu Lys Leu Asp 
                245                 250                 255     
Phe Lys Asp Arg Val Gln Ser Lys Ile Gly Ser Leu Asp Asn Ile Thr 
            260                 265                 270         
His Val Pro Gly Gly Gly Asn Lys Lys Ile Glu Thr His Lys Leu Thr 
        275                 280                 285             
Phe Arg Glu Asn Ala Lys Ala Lys Thr Asp His Gly Ala Glu Ile Val 
    290                 295                 300                 
Tyr Lys Ser Pro Val Val Ser Gly Asp Thr Ser Pro Arg His Leu Ser 
305                 310                 315                 320 
Asn Val Ser Ser Thr Gly Ser Ile Asp Met Val Asp Ser Pro Gln Leu 
                325                 330                 335     
Ala Thr Leu Ala Asp Glu Val Ser Ala Ser Leu Ala Lys Gln Gly Leu 
            340                 345                 350         



<210> 52
<211> 297
<212> PRT
<213> Artificial Sequence


<220> 
<223> W-Tau with no inserts and 3 microtubule-binding domains (W-T30)

<400> 52
Met Ala Glu Pro Arg Gln Glu Phe Glu Val Met Glu Asp His Ala Gly 
1               5                   10                  15      
Thr Tyr Gly Leu Gly Asp Arg Lys Asp Gln Gly Gly Tyr Thr Met His 
            20                  25                  30          
Gln Asp Gln Glu Gly Asp Thr Asp Ala Gly Leu Lys Ala Glu Glu Ala 
        35                  40                  45              
Gly Ile Gly Asp Thr Pro Ser Leu Glu Asp Glu Ala Ala Gly His Val 
    50                  55                  60                  
Thr Gln Ala Arg Met Val Ser Lys Ser Lys Asp Gly Thr Gly Ser Asp 
65                  70                  75                  80  
Asp Lys Lys Ala Lys Gly Ala Asp Gly Lys Thr Lys Ile Ala Thr Pro 
                85                  90                  95      
Arg Gly Ala Ala Pro Pro Gly Gln Lys Gly Gln Ala Asn Ala Thr Arg 
            100                 105                 110         
Ile Pro Ala Lys Thr Pro Pro Ala Pro Lys Thr Pro Pro Ser Ser Gly 
        115                 120                 125             
Glu Pro Pro Lys Ser Gly Asp Arg Ser Gly Tyr Ser Ser Pro Gly Ser 
    130                 135                 140                 
Pro Gly Thr Pro Gly Ser Arg Ser Arg Thr Pro Ser Leu Pro Thr Pro 
145                 150                 155                 160 
Pro Thr Arg Glu Pro Lys Lys Val Ala Val Val Arg Thr Pro Pro Lys 
                165                 170                 175     
Ser Pro Ser Ser Ala Lys Ser Arg Leu Gln Thr Ala Pro Val Pro Met 
            180                 185                 190         
Pro Asp Leu Lys Asn Val Lys Ser Lys Ile Gly Ser Thr Glu Asn Leu 
        195                 200                 205             
Lys His Gln Pro Gly Gly Gly Lys Val Gln Ile Val Tyr Lys Pro Val 
    210                 215                 220                 
Asp Leu Ser Lys Val Thr Ser Lys Cys Gly Ser Leu Gly Asn Ile His 
225                 230                 235                 240 
His Lys Pro Gly Gly Gly Gln Val Glu Val Lys Ser Glu Lys Leu Asp 
                245                 250                 255     
Phe Lys Asp Arg Val Gln Ser Lys Ile Gly Ser Leu Asp Asn Ile Thr 
            260                 265                 270         
His Val Pro Gly Gly Gly Asn Lys Lys Val Lys Gly Val Gly Trp Val 
        275                 280                 285             
Gly Cys Cys Pro Trp Val Tyr Gly His 
    290                 295         

<210> 53
<211> 1161
<212> DNA
<213> Artificial Sequence


<220> 
<223> TIR-MAPT cDNA with 2 inserts and 4 repetitions

<400> 53
atggctgagc cccgccagga gttcgaagtg atggaagatc acgctgggac gtacgggttg      60

ggggacagga aagatcaggg gggctacacc atgcaccaag accaagaggg tgacacggac     120

gctggcctga aagaatctcc cctgcagacc cccactgagg acggatctga ggaaccgggc     180

tctgaaacct ctgatgctaa gagcactcca acagcggaag atgtgacagc acccttagtg     240

gatgagggag ctcccggcaa gcaggctgcc gcgcagcccc acacggagat cccagaagga     300

accacagctg aagaagcagg cattggagac acccccagcc tggaagacga agctgctggt     360

cacgtgaccc aagctcgcat ggtcagtaaa agcaaagacg ggactggaag cgatgacaaa     420

aaagccaagg gggctgatgg taaaacgaag atcgccacac cgcggggagc agcccctcca     480

ggccagaagg gccaggccaa cgccaccagg attccagcaa aaaccccgcc cgctccaaag     540

acaccaccca gctctggtga acctccaaaa tcaggggatc gcagcggcta cagcagcccc     600

ggctccccag gcactcccgg cagccgctcc cgcaccccgt cccttccaac cccacccacc     660

cgggagccca agaaggtggc agtggtccgt actccaccca agtcgccgtc ttccgccaag     720

agccgcctgc agacagcccc cgtgcccatg ccagacctga agaatgtcaa gtccaagatc     780

ggctccactg agaacctgaa gcaccagccg ggaggcggga aggtgcagat aattaataag     840

aagctggatc ttagcaacgt ccagtccaag tgtggctcaa aggataatat caaacacgtc     900

ccgggaggcg gcagtgtgca aatagtctac aaaccagttg acctgagcaa ggtgacctcc     960

aagtgtggct cattaggcaa catccatcat aaaccaggag gtggccaggt ggaagtaaaa    1020

tctgagaagc ttgacttcaa ggacagagtc cagtcgaaga ttgggtccct ggacaatatc    1080

acccacgtcc ctggcggagg aaataaaaag gtaaaggggg tagggtgggt tggatgctgc    1140

ccttgggtat atgggcatta a                                              1161


<210> 54
<211> 386
<212> PRT
<213> Artificial Sequence


<220> 
<223> W-T42 protein sequence

<400> 54
Met Ala Glu Pro Arg Gln Glu Phe Glu Val Met Glu Asp His Ala Gly 
1               5                   10                  15      
Thr Tyr Gly Leu Gly Asp Arg Lys Asp Gln Gly Gly Tyr Thr Met His 
            20                  25                  30          
Gln Asp Gln Glu Gly Asp Thr Asp Ala Gly Leu Lys Glu Ser Pro Leu 
        35                  40                  45              
Gln Thr Pro Thr Glu Asp Gly Ser Glu Glu Pro Gly Ser Glu Thr Ser 
    50                  55                  60                  
Asp Ala Lys Ser Thr Pro Thr Ala Glu Asp Val Thr Ala Pro Leu Val 
65                  70                  75                  80  
Asp Glu Gly Ala Pro Gly Lys Gln Ala Ala Ala Gln Pro His Thr Glu 
                85                  90                  95      
Ile Pro Glu Gly Thr Thr Ala Glu Glu Ala Gly Ile Gly Asp Thr Pro 
            100                 105                 110         
Ser Leu Glu Asp Glu Ala Ala Gly His Val Thr Gln Ala Arg Met Val 
        115                 120                 125             
Ser Lys Ser Lys Asp Gly Thr Gly Ser Asp Asp Lys Lys Ala Lys Gly 
    130                 135                 140                 
Ala Asp Gly Lys Thr Lys Ile Ala Thr Pro Arg Gly Ala Ala Pro Pro 
145                 150                 155                 160 
Gly Gln Lys Gly Gln Ala Asn Ala Thr Arg Ile Pro Ala Lys Thr Pro 
                165                 170                 175     
Pro Ala Pro Lys Thr Pro Pro Ser Ser Gly Glu Pro Pro Lys Ser Gly 
            180                 185                 190         
Asp Arg Ser Gly Tyr Ser Ser Pro Gly Ser Pro Gly Thr Pro Gly Ser 
        195                 200                 205             
Arg Ser Arg Thr Pro Ser Leu Pro Thr Pro Pro Thr Arg Glu Pro Lys 
    210                 215                 220                 
Lys Val Ala Val Val Arg Thr Pro Pro Lys Ser Pro Ser Ser Ala Lys 
225                 230                 235                 240 
Ser Arg Leu Gln Thr Ala Pro Val Pro Met Pro Asp Leu Lys Asn Val 
                245                 250                 255     
Lys Ser Lys Ile Gly Ser Thr Glu Asn Leu Lys His Gln Pro Gly Gly 
            260                 265                 270         
Gly Lys Val Gln Ile Ile Asn Lys Lys Leu Asp Leu Ser Asn Val Gln 
        275                 280                 285             
Ser Lys Cys Gly Ser Lys Asp Asn Ile Lys His Val Pro Gly Gly Gly 
    290                 295                 300                 
Ser Val Gln Ile Val Tyr Lys Pro Val Asp Leu Ser Lys Val Thr Ser 
305                 310                 315                 320 
Lys Cys Gly Ser Leu Gly Asn Ile His His Lys Pro Gly Gly Gly Gln 
                325                 330                 335     
Val Glu Val Lys Ser Glu Lys Leu Asp Phe Lys Asp Arg Val Gln Ser 
            340                 345                 350         
Lys Ile Gly Ser Leu Asp Asn Ile Thr His Val Pro Gly Gly Gly Asn 
        355                 360                 365             
Lys Lys Val Lys Gly Val Gly Trp Val Gly Cys Cys Pro Trp Val Tyr 
    370                 375                 380                 
Gly His 
385     

<210> 55
<211> 233
<212> DNA
<213> Artificial Sequence


<220> 
<223> Exon12-Exon13 (MAPT, NM_005910.5)

<400> 55
gaggtggcca ggtggaagta aaatctgaga agcttgactt caaggacaga gtccagtcga     60

agattgggtc cctggacaat atcacccacg tccctggcgg aggaaataaa aagattgaaa    120

cccacaagct gaccttccgc gagaacgcca aagccaagac agaccacggg gcggagatcg    180

tgtacaagtc gccagtggtg tctggggaca cgtctccacg gcatctcagc aat           233


<210> 56
<211> 233
<212> DNA
<213> Artificial Sequence


<220> 
<223> Exon12-Intron 12 (TIR-MAPT)

<400> 56
gaggtggcca ggtggaagta aaatctgaga agcttgactt caaggacaga gtccagtcga     60

agattgggtc cctggacaat atcacccacg tccctggcgg aggaaataaa aaggtaaagg    120

gggtagggtg ggttggatgc tgcccttggg tatatgggca ttaatcaagt tgagtggaca    180

aaggctggtc cagttcccag aggaggaaaa cagaggcttc tgtgttgact ggc           233


<210> 57
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> D1

<400> 57
Leu Tyr Ile Trp Val Gln 
1               5       

<210> 58
<211> 8
<212> PRT
<213> Artificial Sequence


<220> 
<223> D1B

<400> 58
Leu Tyr Ile Trp Ile Trp Arg Thr 
1               5               

<210> 59
<211> 8
<212> PRT
<213> Artificial Sequence


<220> 
<223> D1D

<400> 59
Leu Tyr Ile Trp Ile Gln Lys Thr 
1               5               

<210> 60
<211> 57
<212> DNA
<213> Artificial Sequence


<220> 
<223> MAPT gene region Fig. 1

<400> 60
aaaaaggtaa agggggtagg gtgggttgga tgctgccctt gggtatatgg gcattaa        57


<210> 61
<211> 10
<212> PRT
<213> Artificial Sequence


<220> 
<223> w-Tau peptide fragment (10aa)

<400> 61
Val Gly Trp Val Gly Cys Cys Pro Trp Val 
1               5                   10  

<210> 62
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> w-Tau peptide fragment (6aa)

<400> 62
Pro Trp Val Tyr Gly His 
1               5       

<210> 63
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> w-Tau peptide fragment (6aa) alternative

<400> 63
Gly Trp Val Tyr Gly His 
1               5       

