                         SEQUENCE LISTING

<110>  Nanjing University
 
<120>  CONJUGATE AND THE PREPARING METHOD AND USE THEREOF

<130>  0230-PA-004

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SGR-beads-1 sequence

<400>  1
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctc                   49


<210>  2
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SGR-tag-1 sequence

<400>  2
tggagttcag acgtgtgctc ttccgatctg ttgtcaagat gctaccgttc agag             54


<210>  3
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SGR-beads-2 sequence

<400>  3
aatgatacgg cgaccaccga gatct                                             25


<210>  4
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SGR-tag-2 sequence

<400>  4
caagcagaag acggcatacg agattcgcct tagtgactgg agttcagacg tgtgctc          57


<210>  5
<211>  124
<212>  PRT
<213>  Artificial Sequence

<220>
<223>   Engineered Neo2

<400>  5

Met Leu Val Asn Arg Ile Cys Gly Lys Gly Ile Asp Gly Gly Ser Pro 
1               5                   10                  15      


Lys Lys Lys Ile Gln Leu His Ala Glu His Ala Leu Tyr Asp Ala Leu 
            20                  25                  30          


Met Ile Leu Asn Ile Val Lys Thr Asn Ser Pro Pro Ala Glu Glu Lys 
        35                  40                  45              


Leu Glu Asp Tyr Ala Phe Asn Phe Glu Leu Ile Leu Glu Glu Ile Ala 
    50                  55                  60                  


Arg Leu Phe Glu Ser Gly Asp Gln Lys Asp Glu Ala Glu Lys Ala Lys 
65                  70                  75                  80  


Arg Met Lys Glu Trp Met Lys Arg Ile Lys Thr Thr Ala Ser Glu Asp 
                85                  90                  95      


Glu Gln Glu Glu Met Ala Asn Ala Ile Ile Thr Ile Leu Gln Ser Trp 
            100                 105                 110         


Ile Phe Ser Ala Val Asp His His His His His His 
        115                 120                 


<210>  6
<211>  143
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered Nb-Pd-L1

<400>  6

Met Asp Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Lys Met Ser Ser 
            20                  25                  30          


Arg Arg Cys Met Ala Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu 
        35                  40                  45              


Arg Val Ala Lys Leu Leu Thr Thr Ser Gly Ser Thr Tyr Leu Ala Asp 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Gln Asn Asn Ala Lys Ser Thr 
65                  70                  75                  80  


Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Met Tyr 
                85                  90                  95      


Tyr Cys Ala Ala Asp Ser Phe Glu Asp Pro Thr Cys Thr Leu Val Thr 
            100                 105                 110         


Ser Ser Gly Ala Phe Gln Tyr Trp Gly Gln Gly Thr Gln Val Thr Val 
        115                 120                 125             


Ser Ser Leu Pro Glu Thr Gly Gly Cys His His His His His His 
    130                 135                 140             


<210>  7
<211>  243
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered GFP

<400>  7

Met Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Arg Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Asn Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu 
    50                  55                  60                  


Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Arg 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Ser Phe Lys Asp Asp Gly Thr Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Cys Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Phe Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Ala Asn Phe Lys Ile Arg His Asn Val Glu Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Val Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
    210                 215                 220                 


Thr Ala Ala Gly Ile Thr His Gly Gly Gly Gly Leu Glu His His His 
225                 230                 235                 240 


His His His 
            


<210>  8
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Trastuzumab light chain:

<400>  8

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  9
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Trastuzumab heavy chain

<400>  9

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile Lys Asp Thr 
            20                  25                  30          


Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val 
        115                 120                 125             


Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala 
    130                 135                 140                 


Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser 
145                 150                 155                 160 


Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val 
                165                 170                 175     


Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro 
            180                 185                 190         


Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys 
        195                 200                 205             


Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp 
    210                 215                 220                 


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly 
225                 230                 235                 240 


Pro Ser Val Phe Leu Phe Pro Pro Lys Asp Thr Leu Met Ile Ser Arg 
                245                 250                 255     


Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 
            260                 265                 270         


Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 
        275                 280                 285             


Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val 
    290                 295                 300                 


Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 
305                 310                 315                 320 


Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr 
                325                 330                 335     


Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 
            340                 345                 350         


Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys 
        355                 360                 365             


Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 
    370                 375                 380                 


Leu Thr Gln Pro Glu Gly Lys Asn Asn Thr Thr Lys Pro Pro Lys Val 
385                 390                 395                 400 


Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ser 
                405                 410                 415     


Arg Lys Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg 
            420                 425                 430         


Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Ser 
        435                 440                 445             


Leu Lys Ser Pro Lys 
    450             


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  20ntssDNA

<400>  10
agcagcacag aggtcagatg                                                   20


<210>  11
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  59ntssDNA

<400>  11
tgtcaagatg ctaccgttca gagcgcaaga cactccacaa aaaaaaaaaa aaaaaaaaa        59


