                         SEQUENCE LISTING

<110>  Indi Molecular, Inc.
       Eliasen, Anders  R.
 
<120>  KRAS-Specific Cyclic Peptides, Compositions, and Methods of 
       Making and Using

<130>  INDI 112

<150>  63/170,261
<151>  2021-04-02

<160>  47    

<170>  PatentIn version 3.5

<210>  1
<211>  189
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Human-derived wild-type KRAS protein

<400>  1

Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Gly Gly Val Gly Lys 
1               5                   10                  15      


Ser Ala Leu Thr Ile Gln Leu Ile Gln Asn His Phe Val Asp Glu Tyr 
            20                  25                  30          


Asp Pro Thr Ile Glu Asp Ser Tyr Arg Lys Gln Val Val Ile Asp Gly 
        35                  40                  45              


Glu Thr Cys Leu Leu Asp Ile Leu Asp Thr Ala Gly Gln Glu Glu Tyr 
    50                  55                  60                  


Ser Ala Met Arg Asp Gln Tyr Met Arg Thr Gly Glu Gly Phe Leu Cys 
65                  70                  75                  80  


Val Phe Ala Ile Asn Asn Thr Lys Ser Phe Glu Asp Ile His His Tyr 
                85                  90                  95      


Arg Glu Gln Ile Lys Arg Val Lys Asp Ser Glu Asp Val Pro Met Val 
            100                 105                 110         


Leu Val Gly Asn Lys Cys Asp Leu Pro Ser Arg Thr Val Asp Thr Lys 
        115                 120                 125             


Gln Ala Gln Asp Leu Ala Arg Ser Tyr Gly Ile Pro Phe Ile Glu Thr 
    130                 135                 140                 


Ser Ala Lys Thr Arg Gln Arg Val Glu Asp Ala Phe Tyr Thr Leu Val 
145                 150                 155                 160 


Arg Glu Ile Arg Gln Tyr Arg Leu Lys Lys Ile Ser Lys Glu Glu Lys 
                165                 170                 175     


Thr Pro Gly Cys Val Lys Ile Lys Lys Cys Ile Ile Met 
            180                 185                 


<210>  2
<211>  189
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Human-derived KRAS (G12D) protein

<400>  2

Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Asp Gly Val Gly Lys 
1               5                   10                  15      


Ser Ala Leu Thr Ile Gln Leu Ile Gln Asn His Phe Val Asp Glu Tyr 
            20                  25                  30          


Asp Pro Thr Ile Glu Asp Ser Tyr Arg Lys Gln Val Val Ile Asp Gly 
        35                  40                  45              


Glu Thr Cys Leu Leu Asp Ile Leu Asp Thr Ala Gly Gln Glu Glu Tyr 
    50                  55                  60                  


Ser Ala Met Arg Asp Gln Tyr Met Arg Thr Gly Glu Gly Phe Leu Cys 
65                  70                  75                  80  


Val Phe Ala Ile Asn Asn Thr Lys Ser Phe Glu Asp Ile His His Tyr 
                85                  90                  95      


Arg Glu Gln Ile Lys Arg Val Lys Asp Ser Glu Asp Val Pro Met Val 
            100                 105                 110         


Leu Val Gly Asn Lys Cys Asp Leu Pro Ser Arg Thr Val Asp Thr Lys 
        115                 120                 125             


Gln Ala Gln Asp Leu Ala Arg Ser Tyr Gly Ile Pro Phe Ile Glu Thr 
    130                 135                 140                 


Ser Ala Lys Thr Arg Gln Arg Val Glu Asp Ala Phe Tyr Thr Leu Val 
145                 150                 155                 160 


Arg Glu Ile Arg Gln Tyr Arg Leu Lys Lys Ile Ser Lys Glu Glu Lys 
                165                 170                 175     


Thr Pro Gly Cys Val Lys Ile Lys Lys Cys Ile Ile Met 
            180                 185                 


<210>  3
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence for V1-V2-V3-V4-V5 of the Cyclic Peptide that 
       binds to KRAS (G12D) protein


<220>
<221>  MOD_RES
<222>  (3)..(4)
<223>  guanidinophenylalanine (guanidinoF or Gnf)

<400>  3

Gln Gln Phe Phe 
1               


<210>  4
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence for V1-V2-V3-V4-V5 of the Cyclic Peptide that 
       binds to KRAS (G12D) protein


<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  guanidinophenylalanine (guanidinoF or Gnf)

<400>  4

Leu Arg Gly Phe 
1               


<210>  5
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence for V1-V2-V3-V4-V5 of the Cyclic Peptide that 
       binds to KRAS (G12D) protein


<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  guanidinophenylalanine (guanidinoF or Gnf)

<400>  5

Leu Arg Gly Asp Phe 
1               5   


<210>  6
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence for V1-V2-V3-V4-V5 of the Cyclic Peptide that 
       binds to KRAS (G12D) protein


<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  Bromophenylalanine

<400>  6

Leu Arg Gly Phe 
1               


<210>  7
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence for V1-V2-V3-V4-V5 of the Cyclic Peptide that 
       binds to KRAS (G12D) protein


<220>
<221>  MOD_RES
<222>  (3)..(4)
<223>  guanidinophenylalanine

<400>  7

Leu Gln Phe Phe 
1               


<210>  8
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence for V1-V2-V3-V4-V5 of the Cyclic Peptide that 
       binds to KRAS (G12D) protein


<220>
<221>  MOD_RES
<222>  (3)..(4)
<223>  guanidinophenylalanine (guanidinoF or Gnf)

<400>  8

Gln Arg Phe Phe 
1               


<210>  9
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>   Synthetic sequence for V1-V2-V3-V4-V5 of the Cyclic Peptide that
       binds to KRAS (G12D) protein


<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  guanidinophenylalanine (guanidinoF or Gnf)

<400>  9

Gln Gln Gly Phe 
1               


<210>  10
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence for V1-V2-V3-V4-V5 of the Cyclic Peptide that 
       binds to KRAS (G12D) protein


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  guanidinophenylalanine (guanidinoF or Gnf)

<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  Bromophenylalanine

<400>  10

Gln Gln Phe Phe 
1               


<210>  11
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence for V1-V2-V3-V4-V5 of the Cyclic Peptide that 
       binds to KRAS (G12D) protein


<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  guanidinophenylalanine (guanidinoF or Gnf)

<400>  11

Gln Arg Gly Phe 
1               


<210>  12
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence for V1-V2-V3-V4-V5 of the Cyclic Peptide that 
       binds to KRAS (G12D) protein


<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  guanidinophenylalanine (guanidinoF or Gnf)

<400>  12

Leu Gln Gly Phe 
1               


<210>  13
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence for V1-V2-V3-V4-V5 of the Cyclic Peptide that 
       binds to KRAS (G12D) protein


<220>
<221>  MOD_RES
<222>  (3)..(4)
<223>  guanidinophenylalanine (guanidinoF or Gnf)

<400>  13

Leu Arg Phe Phe 
1               


<210>  14
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence for targeted epitope region of KRAS protein 
       (Epitope 1)

<400>  14

Ile Leu Asp Thr Ala Gly Gln Glu Glu Tyr Ser Ala Met Arg Asp Gln 
1               5                   10                  15      


Tyr 
    


<210>  15
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence for V1-V2-V3-V4-V5 of the Cyclic Peptide that 
       binds to KRAS (G12D) protein


<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  guanidinophenylalanine (guanidinoF or Gnf)

<400>  15

Gln Gln Gly Asp Phe 
1               5   


<210>  16
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence for V1-V2-V3-V4-V5 of the Cyclic Peptide that 
       binds to KRAS (G12D) protein


<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  guanidinophenylalanine (guanidinoF or Gnf)

<400>  16

Gln Arg Gly Asp Phe 
1               5   


<210>  17
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence for V1-V2-V3-V4-V5 of the Cyclic Peptide that 
       binds to KRAS (G12D) protein


<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  guanidinophenylalanine (guanidinoF or Gnf)

<400>  17

Leu Gln Gly Asp Phe 
1               5   


<210>  18
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence for V1-V2-V3-V4-V5 of the Cyclic Peptide that 
       binds to KRAS (G12D) protein


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  guanidinophenylalanine (guanidinoF or Gnf)

<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  guanidinophenylalanine (guanidinoF or Gnf)

<400>  18

Leu Arg Phe Asp Phe 
1               5   


<210>  19
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence for V1-V2-V3-V4-V5 of the Cyclic Peptide that 
       binds to KRAS (G12D) protein


<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  Bromophenylalanine

<400>  19

Leu Arg Gly Asp Phe 
1               5   


<210>  20
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence for V1-V2-V3-V4-V5 of the Cyclic Peptide that 
       binds to KRAS (G12D) protein


<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  Bromophenylalanine

<400>  20

Gln Gln Gly Phe 
1               


<210>  21
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence for V1-V2-V3-V4-V5 of the Cyclic Peptide that 
       binds to KRAS (G12D) protein


<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  Bromophenylalanine

<400>  21

Gln Arg Gly Phe 
1               


<210>  22
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence for V1-V2-V3-V4-V5 of the Cyclic Peptide that 
       binds to KRAS (G12D) protein


<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  Bromophenylalanine

<400>  22

Leu Gln Gly Phe 
1               


<210>  23
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence for V1-V2-V3-V4-V5 of the Cyclic Peptide that 
       binds to KRAS (G12D) protein


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  guanidinophenylalanine (guanidinoF or Gnf)

<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  Bromophenylalanine

<400>  23

Leu Arg Phe Phe 
1               


<210>  24
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence for V1-V2-V3-V4-V5 of the Cyclic Peptide that 
       binds to KRAS Protein


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  guanidinophenylalanine (guanidinoF or Gnf)

<400>  24

Gln Asn Phe Tyr 
1               


<210>  25
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence that is NOT V1-V2-V3-V4-V5 of the Cyclic 
       Peptide that binds to KRAS Protein

<400>  25

Asn Asp Glu Thr Tyr 
1               5   


<210>  26
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence that is NOT V1-V2-V3-V4-V5 of the Cyclic 
       Peptide that binds to KRAS Protein

<400>  26

Pro Ser Glu Glu Gly 
1               5   


<210>  27
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence that is NOT V1-V2-V3-V4-V5 of the Cyclic 
       Peptide that binds to KRAS Protein

<400>  27

Ser Glu Glu Gly Gly 
1               5   


<210>  28
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence that is NOT V1-V2-V3-V4-V5 of the Cyclic 
       Peptide that binds to KRAS Protein

<400>  28

Glu Gly Thr Gly Thr 
1               5   


<210>  29
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence that is not V1-V2-V3-V4-V5 of the Cyclic 
       Peptide that binds to KRAS Protein

<400>  29

Tyr Glu Gln Gly Glu 
1               5   


<210>  30
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence that is NOT V1-V2-V3-V4-V5 of the Cyclic 
       Peptide that binds to KRAS Protein

<400>  30

Tyr Gly Glu Gln Glu 
1               5   


<210>  31
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence that is NOT V1-V2-V3-V4-V5 of the Cyclic 
       Peptide that binds to KRAS Protein

<400>  31

Leu Arg Gly Asp Arg 
1               5   


<210>  32
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence that is NOT V1-V2-V3-V4-V5 of the Cyclic 
       Peptide that binds to KRAS Protein

<400>  32

Gln Glu Lys Pro Pro 
1               5   


<210>  33
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence that is NOT V1-V2-V3-V4-V5 of the Cyclic 
       Peptide that binds to KRAS Protein

<400>  33

Glu Leu Thr Phe Gly 
1               5   


<210>  34
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence that is NOT V1-V2-V3-V4-V5 of the Cyclic 
       Peptide that binds to KRAS Protein

<400>  34

Val Arg Gly Asp Arg 
1               5   


<210>  35
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence that is NOT V1-V2-V3-V4-V5 of the Cyclic 
       Peptide that binds to KRAS Protein

<400>  35

Leu Arg Gly Pro Arg 
1               5   


<210>  36
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence that is NOT V1-V2-V3-V4-V5 of the Cyclic 
       Peptide that binds to KRAS Protein

<400>  36

Leu Arg Gly Glu Arg 
1               5   


<210>  37
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence that is NOT V1-V2-V3-V4-V5 of the Cyclic 
       Peptide that binds to KRAS Protein


<220>
<221>  MOD_RES
<222>  (2)..(2)
<223>  Homoarginine (homoArg or homoR)

<400>  37

Leu Arg Gly Asp Arg 
1               5   


<210>  38
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence that is NOT V1-V2-V3-V4-V5 of the Cyclic 
       Peptide that binds to KRAS Protein


<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  Homoarginine (HomoArg or homoR)

<400>  38

Leu Arg Gly Asp Arg 
1               5   


<210>  39
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence for a poly-histidine

<400>  39

His His His His His His 
1               5       


<210>  40
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence for a poly-arginine

<400>  40

Arg Arg Arg Arg Arg Arg 
1               5       


<210>  41
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence that is NOT V1-V2-V3-V4-V5 of the Cyclic 
       Peptide that binds to KRAS Protein


<220>
<221>  MOD_RES
<222>  (2)..(2)
<223>  guanidinophenylalanine (guanidinoF or Gnf)

<400>  41

Leu Arg Gly Asp Arg 
1               5   


<210>  42
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence that is NOT V1-V2-V3-V4-V5 of the Cyclic 
       Peptide that binds to KRAS Protein


<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  guanidinophenylalanine (guanidinoF or Gnf)

<400>  42

Leu Arg Gly Ala Phe 
1               5   


<210>  43
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence that is NOT V1-V2-V3-V4-V5 of the Cyclic 
       Peptide that binds to KRAS Protein

<400>  43

Leu Arg Gly Asn Arg 
1               5   


<210>  44
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence that is NOT V1-V2-V3-V4-V5 of the Cyclic 
       Peptide that binds to KRAS Protein

<400>  44

Leu Arg Gly Gln Arg 
1               5   


<210>  45
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence that is NOT V1-V2-V3-V4-V5 of the Cyclic 
       Peptide that binds to KRAS Protein

<400>  45

Leu Arg Gly Ala Arg 
1               5   


<210>  46
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence for V1-V2-V3-V4-V5 of the Cyclic Peptide that 
       binds to KRAS (G12D) protein

<400>  46

Trp Lys Tyr Phe 
1               


<210>  47
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence for targeted epitope region of KRAS protein 
       (Epitope 1)

<400>  47

Asn His Phe Val Asp Glu Tyr Asp Pro Thr Ile Glu Asp Ser Tyr 
1               5                   10                  15  


